EL301 Manual Update PN 3011000, Rev. C.

The Seiko printer information in your manual has been replaced as follows:

• Page 2-18, Table 2.4-1. Model EL 301 Optional Accessories:

OPTIONAL PART
ACCESSORIES NUMBER NOTES

Citizen Printer 97128 40-column printer.

Citizen Replacement
Printer Paper 97129 For use with Citizen printer.

Please call Bio-Tek’s Technical Assistance Center at 800-242-4685 if you have any questions.
BIO-TEK® INSTRUMENTS. INC.

MICROWELL STRIP READER

MODEL EL301

OPERATOR’S MANUAL

MANUAL PART NUMBER 3011000

REVISION C

MAY 1993

BIO-TEK INSTRUMENTS, INC.

HIGHLAND PARK, BOX 998
WINOOSKI, VT 05404-0998 USA
802-655-4040
800-451-5172
800-242-4685 (SERVICE)
TELEX 94-0136 BIO TEK SHVT
FAX 802-655-7941

0-1
 DOCUMENT REVISION RECORD

Revision, date, and section Changes

A 12/28/1989 Original version.

B 8/29/91 Lamp / Filter replacement

procedure, pages, pages 5-3
through 5-9

C 5/1/93 2-3, 2-4 Updated part numbers in

Accessories Lists

Bio-Tek® is a registered trademark of Bio-Tek Instruments, Inc.

0-2
 TABLE OF CONTENTS

Page

0-2 DOCUMENT REVISION RECORD

0-4 TABLE OF CONTENTS
0-7 LIST OF FIGURES
0-8 LIST OF TABLES
0-9 INTRODUCTION TO THE MODEL EL301 OPERATOR’S
MANUAL

1-1 1 MODEL EL301 GENERAL INFORMATION

1-1 1.1 Summary of Features

1-2 1.2 Short-Form Instructions

2-1 2 MODEL EL301 DESCRIPTION

2-1 2.1 Specifications

2-2 2.2 Description and Location of Model EL301 Components

2-6 2.2.1 Front Panel

2-11 2.2.1.1 Filter Paddle

2-13 2.2.1.2 Microwell Strip Carrier

2-15 2.2.2 Rear Panel

2-15 2.2.3 Side Panels

2-17 2.3 Accessories

2-18 2.4 Optional Accessories

3-1 3 INSTALLATION AND CONNECTION TO

PERIPHERAL DEVICES

3-1 3.1 Unpacking and Inspection

3-2 3.2 Electrical Connections

3-2 3.2.1 Wall Socket

3-2 3.2.2 Battery Pack

3-4 3.3 Connecting the Model EL301 to a Printer

3-6 3.4 Connecting the Model EL301 to an External Computer
3-6 3.5 Installing Interference Filters in the Filter Paddle

0-3
 TABLE OF CONTENTS (CONTINUED)

Page

4-1 4 OPERATION

4-1 4.1 Introduction

4-1 4.2 Preliminary Procedures
4-2 4.3 Definitions

4-2 4.3.1 Display Windows on the Model EL301

4-2 4.3.1.1 User Prompts

4-3 4.3.1.2 Error Messages

4-6 4.3.2 Files

4-7 4.3.2.1 Calculation Formats Available

4-9 4.3.3 Functions Performed by the Model EL301

4-9 4.3.3.1 Blanking and Standards

4-9 4.3.3.1.1 Blanking

4-11 4.3.3.1.2 Standards

4-12 4.3.3.2 Clearing

4-13 4.3.3.3 Reading

4-14 4.3.3.3.1 Single Wavelength versus Dual Wavelength

4-16 4.3.3.3.2 Setting Limits for Absorbance and Concentration Values

4-17 4.3.3.4 Data Reduction

4-17 4.3.3.4.1 Absorbance

4-18 4.3.3.4.2 Ratio
4-20 4.3.3.4.3 Linear Equation
4-22 4.3.3.4.4 Difference

4-22 4.3.3.5 Data Output to Peripheral Devices

4-23 4.4 Operation

4-23 4.4.1 Power ON

0-4
 TABLE OF CONTENTS (CONTINUED)

Page

4-24 4.4.2 Positioning the Microwell Strip Carrier on the Behring

EL301 Guide Rail
4-24 4.4.3 Inserting the Filter Paddle into the Model EL301
4-26 4.4.4 Setting Up a File: Tutorial

4-26 4.4.4.1 Power Up and Calibration

4-28 4.4.4.2 File Number and Memory Location
4-29 4.4.4.3 Microwell Strip Configuration and Wavelength Options

4-30 4.4.4.4 Calculation Options Setup

4-32 4.4.4.4.1 Ratio

4-34 4.4.4.4.2 Linear Equation
4-36 4.4.4.4.3 Difference

4-37 4.4.4.5 Limit Options Setup

4-39 4.4.4.6 Data Output Options Setup

4-40 4.4.5 Reading a Microwell Strip

4-44 4.4.6 Sample Printouts

5-1 5 SAFETY, MAINTENANCE, AND SHIPPING

5-1 5.1 Electrical Safety

5-1 5.2 Maintenance

5-1 5.2.1 Testing the Light Bulb

5-3 5.2.1.1 Replacing the Light Bulb

5-5 5.2.1.2 Recalibrating the Light Beam

5-6 5.2.2 Cleaning Exterior Surfaces

5-6 5.2.3 Cleaning the Optics

5-7 5.3 Storage and Shipping

5-8 5.4 Warranty

6-1 6 TROUBLESHOOTING

0-5
 LIST OF FIGURES

Page

2-3 2.2-1 Front View of the Model EL301

2-12 2.2.1.1-1 Filter Paddle with Interference Filters

2-14 2.2.1.2-1 Microwell Strip Carrier

2-16 2.2.2-1 Rear and Side Panel Connectors for the Model EL301

3-3 3.2-1 Model EL301 Connections

3-5 3.3-1 Schematic for Pin Assignment of Model EL301 Cable
and Connector
3-7 3.4-1 Serial Port Connections for the Model EL301

4-5 4.3.1-1 Display Windows Defined for the Model EL301

4-8 4.3.2-1 Number of Wells Stored in Memory

4-21 4.3.3.4.3-1 Graphic Interpretation of Linear Equation

4-25 4.4.2-1 Positioning the Microwell Strip Carrier on the Model

EL301 Guide Rail

4-45 4.4.6-1 Sample Printouts

5-4 5.2.1.1-1 Light Bulb Assembly

0-6
 LIST OF TABLES

Page

2-17 2.3-1 Model EL301 Accessories

2-18 2.4-1 Model EL301 Optional Accessories

6-1 6-1 Troubleshooting the Model EL301

0-7
This page intentionally left blank.

0-8
 INTRODUCTION TO THE MODEL EL301

OPERATOR’S MANUAL

This manual is the operator’s manual for the Model EL301 Strip Reader. It contains general informa-
tion about the Model EL301, a description of its components, and instructions for its use. It does
not contain instructions or documentation required to service the unit. If a problem develops, the
user should contact Bio-Tek. The user should never attempt to service the unit before consulting
with Bio-Tek service personnel.

The objectives of this manual are to provide:

1. sufficient information about the design of the Model EL301 to enable the user to
understand its use (Sections 1 and 2) as well as short-form instructions (Section 1);

NOTE: The short-form instruction pages are printed on colored paper stock so that
this section can be quickly located within the operator’s manual.

2. guidance for installing the unit and connecting to peripheral devices (Section 3);

3. procedures for operating the Model EL301 (Section 4);

4. procedures for maintaining, storing, and shipping the Model EL301 (Section 5);
and

5. procedures for troubleshooting the Model EL301 (Section 6).

0-9
This page intentionally left blank.

0-10
 1 MODEL EL301 GENERAL INFORMATION

1.1 Summary of Features

The Model EL301 Strip Reader is a compact, light-weight single and

dual wavelength photometer designed to measure and interpret the
absorbances in microwell strip assays. The wavelength of the light used
is determined by the interchangeable filters mounted on the filter
paddles. The Model EL301 is ruggedly designed and has a black exte-
rior of anodized aluminum. In addition, the unit is completely portable
and can be used with an optional battery pack allowing its use at remote
sites not accessible to ordinary readers. The Model EL301 is designed
to be used with both hands: one hand to move the microwell strip
carrier and the other hand to enter the data (the unit rests on 4 non-skid
feet so that it will not slide around when the carrier is moved).

The design of the Model EL301 accommodates flat- or round bottom 8-

well, 12-well, or 16-well (2 x 8) microstrip configurations. The strips
must be 9 mm (0.355") from well center-to-well center. The unit is
designed so that well positions are automatically sensed and the micro-
processor can perform the user-defined operations on the well currently
in the optical measurement channel. The internal microprocessor enables
the user to access user-defined microwell analysis programs via the unit’s
keypad. These programs enable the user to choose:

1. single or dual wavelength

2. absorbance readings, ratio, linear equation (regression),

or difference (for 2 x 8 wells only) calculations.

The Model EL301 has the following features:

1. automatic well location sensing

2. user-defined analysis programs

3. automatic average blanking

4. large 2-line 16-character liquid crystal display (LCD) for user

prompting and data entry

1-1
 5. internal memory that stores up to 12 microplates of absorbance values (1,152
absorbance wells)

6. RS-232C serial computer interface (DCE configuration)

7. parallel printer interface (Centronics-style)

8. battery or line power (supply requirements: 12 V, 0.6 amps)

9. optional: printer and cable, rechargeable battery pack, carrying case, and additional
microwell strip carriers

10. filter paddles with interchangeable filters.

Well locations, absorbance values, and results are displayed on the large LCD. Standards and blanks
may be carried over from strip-to-strip within each program. Data may be stored in the Model
EL301 memory, printed out, or sent to a computer for more detailed analysis.

1.2 Short-Form Instructions

For your convenience we have included new short-form instructions at the end of this section of the
Model EL301 Strip Reader Operator’s Manual. The remainder of this section is devoted to these
Short Form Instructions.

Please take the time to read the manual carefully before using these Short Form Instructions to
operate the Model EL301.

NOTE: The Short Form Instructions are printed on colored stock so that they may be
quickly located within the operator’s manual.

1-2
 ***********************************

SHORT FORM INSTRUCTIONS

***********************************

We also have a new direct toll-free line to our

Service Department; please call: 1-800-242-4685
(1-800-24-BIOTK)

1-3
 MODEL EL301 STRIP READER

“DISPLAY” WINDOW

Memory Clear and available

for Data Storage

Ready to Read Strip Label # of Strip

being read; also, mem-
File # ory location of Stored
Data for Strip #1A.

F1 READY/MC 1A

C2 1.543 +

Well Location on Calculated Result.

Strip #1A Concentration or Cut-off
indication (+, -, or +/-
Optical Density or indeterminate “gray” zone).
Absorbance of Well C2.

(1) The “DISPLAY” window is what the end-user will see during the process of reading a
strip or series of strips.

(2) Each File (in other words: F1, F2, F3, F4) may be programmed independent of each
other. Once each File is programmed, the end-user never needs to reprogram the file
again.

(3) Well Location of Strip being read:

8-well strips (1 x 8): A1 to H1

12-well strips (1 x 12): A1 to A12
16-well strips (2 x 8): A1 to H1, then A2 to H2

(4) Absorbance of Strip being read:

“b” after value indicates BLANK Well (e.g., 1.543b)

“s” after value indicates STANDARD Well location (e.g., 1.543s)
Nothing after value indicates SAMPLE Well location (e.g., 1.543 )

1-4
 “SETUP” WINDOW

The “SET-UP” Window is used ONLY for programming the Strip Reader. Once a program is
defined, it is stored in memory and does not need to be re-entered even if the Strip Reader is
turned off.

TO PROGRAM THE STRIP READER:

(1) Press the DISPLAY/SETUP key once (located on the top, right of the keyboard).

(2) The “SETUP” Window will appear.

(3) To ESCAPE from the “SETUP” Window at any time, press the DISPLAY/SETUP key
once. The “DISPLAY” Window will re-appear.

Cut-off Report (Limit report).

“SETUP” Window is Yes or No indication (“Y” or “N”
defined here. directly beneath “L”).
File # to be
preprogrammed.
Data to be sent out
Serial port. Yes
or No indication.

Data to be sent out

Parallel port. Yes
or No indication.

F1 SETUP L S P

8 S ABSORB N N Y

Type of Strip
to be read:
Type of Data Reduction
8-well (1 x 8) to be performed. For example:
12-well (1 x 12) Display ABSORBANCE readings only.
16-well (2 x 8)

Single or
Dual Wavelength
(“S” or “D”)

1-5
 STRIP READER
PROGRAMMABLE FILES AND DATA STORAGE

DEFINITION OF FILES:

(1) 4 Files are available in each Strip Reader.

(2) Each File may be independently programmed to read a series of strips, store the
absorbance values, and perform data reduction on these absorbances.

(3) Each File is labeled as F1, F2, F3, or F4. Absorbance Data are “stored” within
the File that it is read. For example, strips read while the display shows “F1” are
stored in a Memory Location area designated as F1.

Each File defines Data Reduction Programand Location of Stored Data.

F1 F2 F3 F4

Each File contains 3

Plates, (A, B, C)
where strips are
read and data
stored.

A B C

Each Plate contains 6

Strips, (1, 2, 3,
4, 5, or 6)
where wells are
read and data
stored.

1 2 3 4 5 6

Each Strip contains

8, 12, or
16 Wells.
8 12 16

1-6
The File #, Plate Letter, and Strip # designate the strip being read and also indicate the Data Storage
location of those absorbance values. They are always indicated on the “DISPLAY” window:

F3 READY/MC 2A

This means: Read the strip now in the carrier using the program from FILE #3. This is the 2nd
strip in Plate A. To recall or print data from this strip, the display should appear as
above.

NOTE: If the strip already contains data, the display will show
W1/MF or W2/MF where W1 amd W2 are the filter wavelengths
used and MF means “Memory Full.” Press the CLEAR key to
obtain the preceding “DISPLAY” window.

1-7
 TO PROGRAM A FILE TO READ ABSORBANCE

After the Strip Reader has been turned on and calibrated for the filter wavelengths to be used, per-
form the steps to program a File to read Absorbance.

(1) Press the DISPLAY/SETUP key once. When the “SETUP” window appears, you will note
that the CURSOR is on the 2nd line, far left. The CURSOR indicates which Parameter you
wish to change within that File.

(2) Select Strip Type:

Press the OPTION key until “8” F1 SETUP
appears (8-well). 8

(3) Select Wavelength:

Press the PROMPT key to move the F1 SETUP
CURSOR to the next location. Use the 8 S
OPTION key until “S” appears (Single).

(4) Select Calculation Method:

Press PROMPT then OPTION F1 SETUP
until “ABSORB” appears. 8 S ABSORB

(5) Cutoff or Limit Report:

Press PROMPT then OPTION until “N” F1 SETUP L
appears. This means do not perform a 8 S ABSORB N
limit report on these absorbance values.

(6) Serial Output:

Press PROMPT then OPTION until “N” F1 SETUP L S
appears. This means do not send data 8 S ABSORB N N
out the Serial port.

(7) Parallel Output:

Press PROMPT then OPTION until F1 SETUP L S P
“Y” appears. This means send data out 8 S ABSORB N N Y
the parallel port to a printer.
Make sure the Strip Reader is connected
to a printer.

(8) Press the DISPLAY/SETUP

key once to ESCAPE to the F1 READY/MC 1A
“DISPLAY” window.

You are now ready to read the absorbance values of an 8-well strip in Single wavelength and store
the data in File #1.

1-8
 GETTING READY TO READ A STRIP

The “DISPLAY” window should appear as:

F1 READY/MC 1A

Choose the Strip #, Plate Letter, and File #:

(1) The CURSOR should be located under the Strip #.

Use the OPTION key to progress from 1# to the next strip #.

(2) Press PROMPT to move the CURSOR

underneath the Plate Letter. Use F1 READY/MC 1A
OPTION to change the Plate in
which the Strip is located.

(3) Press PROMPT to move the CURSOR

underneath the FILE #. Use F1 READY/MC 1A
OPTION to change the FILE #.

Place the Strip Carrier with an 8-well strip onto the guide rail.
The Well Location should be indicated on the DISPLAY:

F1 READY/MC 1A
C1 __________ __

NOTE: If the well location has been set up as a STANDARD well, the display will be:

F1 READY/MC 1A
C1 ___________s___

1-9
TO READ THE STRIP:

(1) You must BLANK before reading any STANDARD and SAMPLE absorbance values.
BLANK on Air by removing the Strip Carrier and pressing the BLANK key. BLANK on
a well by moving the Strip Carrier to the Well Location (indicated in the “DISPLAY”
window), and pressing BLANK.

You may BLANK on as many wells as you like BEFORE reading any STANDARD wells
(if used) and then reading the SAMPLE wells. BLANKS may be carried over from one
strip to the next.

(2) Move the Strip Carrier to the first SAMPLE well to be read. Press the READ key.

(3) The ABSORBANCE value will immediately appear in the “DISPLAY” window:

F1 READY/MC 1A
B1 1.400

(4) Move the Strip Carrier to the next SAMPLE well to be read. Press READ.

(5) Press the DATA OUT key to print Strip 1A ABSORBANCE values.

1-10
 OTHER PROGRAMS

LINEAR EQUATION

(1) Press the DISPLAY/SETUP key once to change to the “SETUP” window.

(2) Press the PROMPT key to advance

the cursor to the DATA REDUCTION F1 SETUP LSP
position (ABSORB). Press OPTION 8 S LINEQU NNN
until “LINEQU” appears.

(3) Press ENTER to enter into the subroutine.

NOTE: In this subroutine, remember that:

- PROMPT advances the CURSOR to the right during

STANDARD CONCENTRATION selections.

- OPTION provides choices (numbers or letters).

- ENTER stores the programmed data and then advances

to the next parameter.

(4) Choose the number of STANDARDS using the OPTION key (up to 5
STANDARDS), then press ENTER.

- Choose the location of the 1st STANDARD (all other STANDARDS

must follow sequentially) and then press ENTER.

NOTE: After the location of the first STANDARD is entered,

CONCENTRATION UNITS (e.g., UG/ML) are selected. (This display
appears only after the first STANDARD.)

- Enter the concentrations of each standard by using the PROMPT key to advance the
cursor, the OPTION key to enter numbers, and the ENTER key to advance to the next
concentration.

(5) When all parameters have been entered, the window will return to “LINEQU” (as in the
preceding Step #2).

(6) Press the DISPLAY/SETUP key once to return to the “DISPLAY” window.

1-11
(7) BLANK as many wells as desired. BLANKS may be carried over from one strip to the
next.

NOTE: Remember that BLANK wells must be read BEFORE reading

STANDARD wells and that STANDARD wells must be read BEFORE
reading SAMPLE wells.

(8) STANDARDS may be carried over from strip-to-strip, and plate-to-plate. If you are
reading Strip #1 of a new plate, the “DISPLAY” window shows:

NEW STANDARDS?
OPTION OR ENTER

Press ENTER if you wish to read “NEW” STANDARDS into memory.

Press OPTION and the “DISPLAY” window changes to:

SAME STANDARDS?
OPTION OR ENTER

Then press ENTER if you wish to use the same STANDARDS

previously read and already stored in memory. (The OPTION key
can be pressed repeatedly to toggle between “NEW” and “SAME”
STANDARDS.)

1-12
CUT-OFF OR LIMIT REPORT

(1) In the “SETUP” window, press PROMPT to move the CURSOR

to “L” position. Press OPTION until “Y” appears.
Press ENTER; this defines 2 Cut-off values.

F1 SETUP L S P
8 S ABSORB Y N N

(2) If the DATA REDUCTION location is set for “ABSORB,” then the Cut-off
(or Limit) values are in Absorbance Units.

F1 LIMITS SETUP
1ST LIMIT = 0.000

If the DATA REDUCTION location is set for “LINEQU,” the Cut-off

(Limit) values are set for Concentration Units.

F1 LIMITS SETUP
1ST LIMIT = 000.0

Remember to use the PROMPT key to advance the CURSOR, the

OPTION key to select numbers, and the ENTER key to advance to the
second Cut-off (Limit) value.

(3) Two (2) Limit values may be set. The result is shown on the “DISPLAY”
window (or printout) as:

- if below the 1st Limit value

+ if equal to or above the 2nd Limit value
+/- if between the 1st and 2nd Limit (the indeterminate
“gray” zone)

NOTE: If Limit 1 = Limit 2, then only + and - indications are possible.

(4) After the SETUP is complete, press DISPLAY/SETUP to escape to the

“DISPLAY” window.

1-13
DATA OUT AND CLEAR KEYS

(1) The CURSOR located in the “DISPLAY” window indicates which data will be sent out
the Serial or Parallel port. Press PROMPT to advance the cursor from Strip # to Plate
Letter to File #. When the CURSOR is under the Strip #, data for THAT STRIP ONLY is
sent out:

F1 W1/MC 1A

When the CURSOR is under the Plate Letter, data for ALL 6 STRIPS are sent out:

F1 W1/MC 1A

When the CURSOR is under the File #, data for ALL 3 PLATES (3 x
6 strips = 18 strips of data) is sent out:

F1 W1/MC 1A

(2) The CLEAR key is used in the same way as the DATA OUT key.
The CURSOR must be carefully positioned under the data to be
cleared before the key is pressed. As is the preceding Step
#1, the PROMPT key is pressed to move the CURSOR so that the
entire File, the Plate, or just the Strip can be cleared.
All data cleared is permanently erased.

NOTE: In the “SETUP” window, the “Y” must be positioned directly under the “P” to send data
out the parallel port and the “Y” must be positioned directly under the “S” to send data out the serial
port. If the printer is not plugged in or turned on and the reader tries to send data out the parallel
port, the display will show the ERROR MESSAGE:

F1 W1/MF 1A
PRINTER NOT OKAY

If the reader tries to transmit data out of the serial port and no computer is attached, the reader will
hang up. The only way to resume operation from this situation is to start over by turning the unit off
and then on again. Make sure that the “Y” or “N” serial port selection is correct for the configuration
of your equipment!

1-14
 CALIBRATION

The CALIBRATION procedure for the Strip Reader is simplified in recent software revisions (Revi-
sion C and greater, refer to Section 4.4.4.1 to determine revision). Initial CALIBRATION takes
place when the unit is turned on.

(1) When the reader is turned on, the opening display is followed by:

REMOVE CARRIER
THEN HIT ENTER

(2) Press ENTER and the display changes to:

INSERT FILTER W2
THEN HIT ENTER

(3) Push the filter paddle into the receptacle on the front of the reader
until the indicator line and the molded “W2” are flush with the opening
of the filter paddle receptacle. Press ENTER and the display shows:

CALIBRATING
WAVELENGTH W2

(4) When calibration for wavelength W2 is complete the display shows:

INSERT FILTER W1
THEN HIT ENTER

(5) Insert filter paddle all the way into its receptacle and press ENTER:

CALIBRATING
WAVELENGTH W1

(6) Calibration for both filter wavelengths is now complete and the
display changes to an opening display such as:

F1 READY/MC 1A
___

(7) The strip reader is now ready to read at filter W1. The CALIBRATION
procedure for “W2” is used for DUAL WAVELENGTH readings only.

(8) If the filter in the “W2” position is to be used for SINGLE

WAVELENGTH readings, re-insert the “W2” filter into the the “W1”
position on the filter paddle. Recalibrate for this filter by following the
CALIBRATION procedure described in the preceding steps.

1-15
 TO READ DUAL WAVELENGTH

(1) During the “SETUP” procedure, press PROMPT until the CURSOR is positioned
underneath the “S” and then press OPTION to change the wavelength location to “D.”

(2) Press DISPLAY / SETUP once to return to the “DISPLAY” window.

(3) Make sure that filter “W1” is in position under the light beam.

(4) Read BLANKS and STANDARDS as you would for single wavelength. The first line of
the display is slightly different, such that:

F1 DUAL/W1 1A
A1

It is clear that for this display that you are reading at the first
wavelength “W1” for well location “A1.”

NOTE: No absorbance values will be shown on the DISPLAY window

until the 2nd filter wavelength has been read.

(5) When all the wells in the strip have been read at “W1,” press W1/W2 to read at the
second filter wavelength. The “DISPLAY” window will show:

F1 DUAL/W2 1A
A1

(6) Move the filter paddle into position for “W2.” Pull the paddle out of
the paddle receptacle until it “clicks” into position and the indicator
line and molded “W2” is flush with the opening of the receptacle.

(7) Read the BLANK wells for W2 and then read any STANDARD wells. Then proceed to
read the SAMPLE wells. The Absorbance values will be shown on the “DISPLAY”
window.

(8) When all wells for the 1st strip have been read in W2:

(A) Press OPTION to change to the next Strip #.

(B) Push filter W1 back into position.

(C) Press W1/W2 to change the filter wavelength back to “W1.”

1-16
 PRINTOUT EXAMPLE

In the printout below, the user selected:

12-well strip

Blanks: A-1 and A-2

Absorbance Calculation

----------------------------------------------------------------------------------------------------------------------

File 2 Strip 1A

# TYPE O.D.
__ ____ ____

A1 b -0.004
A2 b 0.004
A3 - 0.299
A4 - -0.012
A5 - 0.345
A6 - 1.008
A7 - 0.907
A8 - 0.189
A9 - 0.256
A10 - 0.622
A11 - 0.489
A12 - 1.120

1-17
 PRINTOUT EXAMPLE

In the printout below, the user selected:

2 x 8-well strip

Blanks: A-1 and A-2

Difference Calculation

----------------------------------------------------------------------------------------------------------------------

File 1 Strip 3C

# TYPE O.D. DIFF

__ ____ ____ ______

A1 b 0.004
B1 b -0.004
C1 - 0.299
D1 - -0.012
E1 - 0.345
F1 - 1.008
G1 - 0.907
H1 - 0.189

A2 - 0.657 -0.653
B2 - 0.620 -0.624
C2 - 0.296 0.003
D2 - -0.287 0.275
E2 - 0.986 -0.641
F2 - 1.209 -0.201
G2 - 0.196 0.771
H2 - +.+++ *.***

1-18
 PRINTOUT EXAMPLE

In the printout below, the user selected:

8-well strip
Blank: A-1
Ratio Calculation
C-1 as standard, conc. = 200.0 NG/ML
----------------------------------------------------------------------------------------------------------------------

File 3 Strip 2D

# TYPE O.D. RATIO

__ ____ ____ ______

A1 b 0.000 .0
B1 - 0.604 404.0
C1 s 0.299 200.0
D1 - -0.012 -8.0
E1 - 0.345 130.6
F1 - 1.008 654.2

G1 - 0.907 606.6
H1 - 0.189 126.4
________________________________________________________________________________________________

PRINTOUT EXAMPLE

In the printout below, the user selected:

8-well strip
Blanks: A-1 and B-1
Limits Program
----------------------------------------------------------------------------------------------------------------------
File 4 Strip 3C

Limit1 = +0.200
Limit2 = -0.200

# TYPE O.D. LIMITS

__ ____ ____ ______

A1 b 0.004 +/-
B1 b -0.004 +/-
C1 - 0.299 +
D1 - -0.212 -
E1 - 0.345 +
F1 - 1.008 +
G1 - 0.907 +
H1 - 0.189 +/-

1-19
1-20
 2 MODEL EL301 DESCRIPTION

2.1 Specifications

Microwell Strips 8-well single row strips, 12-well single row strips, and 16-
well double row (2 x 8) strips with round or flat bottoms are
accommodated.

NOTE: All strips must be 9 mm (0.355") from well center to

well center to be accommodated.

Absorbance Range 0 -- 999 Abs (Absorbance Units).

NOTE: Negative optical densities will occur when the well

read blocks less light than the well designated as the blank.

Concentration Range for 000.0 -- 999.9.

RATIO and LINEQU
(Linear Equation)
Calculations

Absorbance Display 0.001 Abs.

Resolution

Accuracy and Repeatability +1% +0.005 Abs of the true optical

(Combined) density relative to air (to 2.000 O.D.) when tested on the
absorption peak of a solution at any wavelength within the
wavelength range specified.

NOTE: Accuracy alone shall be +1% of 1.000 ABS.

Linearity 0 -- 2.000 Abs +1% at 405 nm.

Photodetector Silicon photodiode.

Wavelength Range 400 -- 700 nm.

Wavelength Selection User-interchangeable narrowband interference filters. (Two

filters provided: 405 nm and 490 nm, other filters available
on request. Refer to Table 2.4-1 to order.)

2-1
Light Source Tungsten-halogen bulb with a minimum life of
300,000 readings.

Display 2 x 16-character dot matrix-style alphanumeric liquid

crystal display (LCD) with underline cursor.

Power Requirements 100 VAC +10%, 50/60 Hz, 10 W

115 VAC +10%, 50/60 Hz, 10 W
230 VAC +10%, 50/60 Hz, 10 W

NOTE: Uses a transformer assembly at 12 VDC, 1A

(optional cables available).

Serial Interface Full duplex RS-232C serial interface (with RJ-24 type
connector) at 1200 baud.

Parallel Interface 8-bit parallel interface for the optional printer.

Dimensions 27.9 cm (11") x 15.2 cm (6") x 7.6 cm (3").

Weight 1.4 kg (3 lb).

Environment

Operating Temperature 5o -- 45o C

Storage Temperature -20o -- 60o C

Relative Humidity 0 -- 90% (non-condensing)

Ambient Light Avoid direct sunlight or strong tungsten lighting when

taking readings.

2.2 Description and Location of Model EL301 Components

Figure 2.2-1 is the front view of the Model EL301 microwell strip reader.

1. Optics Arm Shroud: Located on the top of the Model EL301. The light bulb
assembly is housed in the optics arm and uses a tungsten-halogen bulb (refer to
Section 5.2.1 for instructions to replace the light bulb). The ball-bearing detent
mechanism on the underside of the optics arm locks the microwell strip holder into
place as it slides along the guide rail thus ensuring that each well in turn is properly
positioned within the light beam that travels into the filter chamber.

2-2
Figure 2.2-1. Front View of the Model EL301

2-3
2. Optics Arm Shroud Mount Screws: Located in the rear portion of the optics arm.
These recessed screws are removed when the light bulb is replaced. (Refer to
Section 5.2.1 for a more detailed explanation of this maintenance procedure.)

3. Detent Mechanism: Located on the underside of the optics arm and described in
Item 1, preceding.

4. Raised Guide Rail: Located on the front panel of the Model EL301. The
microwell strip carrier slides along this fixed guide rail to ensure that each well is
properly positioned in the light beam path traveling into the filter chamber.

NOTE: The Model EL301 well-position sensing system is located near the portion of
the guide rail that passes under the optics arm. This system enables the reader to
automatically determine the well location by sensing when the magnet embedded in the
microwell strip carrier passes over the system. The well position information is trans-
ferred to the Model EL301 controlling microprocessor. The detent mechanism should
be in place before taking a reading.

If the carrier is not aligned on a well location, no location will appear on the display
and no reading can be taken. (It should be noted that it is possible to fool the unit into
entering data when the carrier is outside of the detent mechanism by passing the
carrier back and forth over the location of the well-sensing magnet.)

5. Filter Paddle: Two interference filters can be mounted in the filter paddle that is
placed into the filter paddle receptacle on the front of the Model EL301. (Refer
to Section 2.2.1.1 for a more detailed description of the filter paddle.)

6. Filter Chamber: Located directly underneath the free end of the optics arm
(nearest the filter paddle receptacle). The filter chamber is aligned with the focused
light beam that eminates from the optics arm, passes through the microwell being
read, and passes through the interference filter positioned in the filter chamber.
The light beam reaches the silicon photodiode as its final destination. The light
energy is converted into an electrical signal and sent directly to the microprocessor.

7. ON/OFF Switch: Located on the left side panel of the Model EL301 as depicted
in the figure. The switch is toggled ON and OFF.

NOTE: The reader can be turned OFF and then turned ON again to recalibrate for
each new filter wavelength or to switch from double to single wavelength readings.
When reading in dual wavelength, pressing the W1/W2 button also functions to
recalibrate the reader for the new filter wavelength.

2-4
8. Non-Skid Rubber Feet (Not Shown): Located on the underside of the unit. The
Model EL301 rests on 4 non-skid rubber feet because the reader is designed to
rest securely on the working surface while the reader is used with 2 hands: one
hand to slide the microwell strip carrier along the guide rail and the other hand to
use the buttons on the keypad.

The Model EL301 front panel is described in the following section.

2-5
2.2.1 Front Panel

This section describes the function and action of the front panel buttons (see Figure 2.2-1 for the
Model EL301 keyboard). These raised buttons are pressure sensitive and emit an audible tone when
pressed.

1. SET-UP Buttons: These buttons are used when the operator wishes to set up new
files. Complete instructions for using the set-up buttons are found in Section 4.

● PROMPT: This button is located in the upper righthand corner of the

Model EL301 front panel. The PROMPT button moves the cursor from left to right
(from parameter to parameter) hence functioning as a forward arrow button in both
the DISPLAY and SET-UP modes. When the cursor has been prompted forward as
far as it can go on the right side of the display it then immediately wraps around to
the far left side of the display.

NOTE: This button can be held down to advance the cursor quickly.

● OPTION: This button is located to the right of the PROMPT button and is used to
cycle through the options within each parameter (that is, the cursor will remain in
the same position on the display but the display itself will change.) This button
functions in both the SET-UP and DISPLAY modes.

During SET-UP, the OPTION button cycles through the options available for the
particular parameter identified by the underline cursor position on the display. If an
option is selected (by pressing ENTER) and requires that a well location or number be
entered, then this button functions as a incremental button. For example, the incre-
ments could be the numbers 0 through 9 or the letters A through H, depending on the
information to be entered.

NOTE: This button will cycle through the options quickly if held down.

● ENTER: Located to the right of the OPTION button. This button is used to select
parameters during the SET-UP mode. When the parameter or option desired is
located using the PROMPT and OPTION buttons, respectively, pressing the
ENTER button enters the choice into the Model EL301 memory.
Depending on the prompting sequence parameter indicated on the display, pressing
ENTER causes the reader to present the operator with choices from a submenu.
When the parameter, option, or location choice has been successfully entered, the
underline cursor advances to the next parameter choice.

2-6
2. DISPLAY/SET-UP: Located in the upper righthand corner next to the ENTER
button. Pressing the DISPLAY/SET-UP button will toggle the display between the
set-up display and the data display.

NOTE: Pressing the DISPLAY/SET-UP button when the LCD window shows the
set-up display changes the display to the data display. The underline cursor defaults to
the file memory location number.

3. DATA Buttons: Located underneath the row of SET-UP buttons in the upper
righthand corner of the Model EL301 front panel.

● DATA OUT: This button sends data out of the printer port and the RS-232C serial
port, depending on the options selected during the file setup. Data can also be
transmitted out the ports by locating the cursor under the File Number or the
Memory Location and pressing the DATA OUT button. The message:

SENDING DATA

will appear on the display during data transmission. If the DATA OUT button is
pressed while the unit is transmitting data, the transmission will be terminated.

NOTE: If DATA OUT is pressed and the printer is not hooked up to the Model
EL301, an error message will appear on the display to inform the operator that trans-
mission was unsuccessful. If the computer is not hooked up to the serial port, the
reader will hang up after it tries to transmit data; the only way to resume operation of
the reader is to turn the unit OFF and then ON again.

● CLEAR: During the Set-Up Mode, pressing the CLEAR button clears the data on
the bottom line of the display and returns to the previously chosen parameter. This
button enables the operator to re-enter data for concentrations and limits. (For
additional information, refer to Section 4.3.3.2.)

During the Display Mode, pressing CLEAR clears the data stored in either the file, the
plate, or the strip, depending on the location of the cursor when CLEAR is pressed.

NOTE: Place the cursor under the data memory location to be cleared before pressing
CLEAR. (An entire file can be cleared instead of just strip if the cursor is placed
under the file designator rather than the strip designator.)

Remember to use the CLEAR button to clear any blanks, standards, or differences that
are not to be carried over to the new file to be read.

2-7
● W1/W2: This button toggles between the interference filter calibration values in
memory.

NOTE: Remember that the unit is recalibrated whenever the Model EL301 is pow-
ered up or reset.

NOTE: The reader must be turned OFF and then turned ON to initiate the
recalibration procedure when switching from double-to-single wavelength or from one
single wavelength to another single wavelength.

The software of the Model EL301 ensures that the microwell strip carrier is removed
before calibration of filters W1 and W2 by displaying an operator message on the
LCD. The software cannot, however, detect if a filter is actually mounted in the filter
paddle or which filter is mounted in the paddle. The operator must ensure that the
proper filter is installed during this calibration procedure.

4. BLANK: This button is positioned to the right of the W1/W2 button. It is used to
instruct the microprocessor to read the current microwell as a blank. When the data
display window is shown on the LCD, this button enables the operator to read the
optical density of the microwell positioned over the filter chamber and use this
value as the blanking value that is subtracted from all other microwell readings. If
there is no carrier in place, the well location is blanked against air.

When a well is successfully blanked the Model EL301 emits one audible tone when
BLANK is pressed. A blanked well shows up on the display or on the printout as the
optical density value immediately followed by the lowercase letter “b.” Blanks can be
carried over from strip-to-strip within the same file by simply continuing to read the
sample wells. A new blank can replace the current blank if BLANK is pressed before
beginning to read a new microwell strip. The new blanking value is subtracted from
all subsequent readings.

Remember to observe the following protocol when using the blanking function of the
Model EL301:

● When the Model EL301 is turned ON or after a blank has been deleted, a
new blanking well must be entered before beginning to read the microwell
strip. An attempt to read the strip before blanking will cause the unit to
display the error message:

NO BLANKING YET

2-8
 ● Blanks must be read first if not already established for the particular file
setup.

● Blanks cannot be read within a strip after the first sample is read.

● Standards wells (if defined in the file setup) must be read immediately after
the blank well(s) is read but before the sample wells are read.

● Blanks and standards entered for the first wavelength, W1, of a dual reading
must also be entered on the same well locations during the second wave
length, W2, reading. The Model EL301 prompts the operator for these
blanks and standards.

● If the microwell strip holder is not aligned on a valid well location, pressing
BLANK does nothing.

5. READ: This button is located in the lefthand corner of the Model EL301 front
panel (to the left of the display window). When all blanks and standards have been
entered and when the strip carrier is in place, then the READ button can be pressed
to obtain the optical density for the well in the light path of the filter chamber.

The Model EL301 emits an audible tone when the well has been read successfully.
The optical density of that particular well is displayed in the LCD window;
remember that the blanking value has been subtracted from the value displayed in
the window. This same well can be read repeatedly by pressing READ repeatedly;
however, only the most current reading is retained in the file memory.

The next well can be read by sliding the carrier to the next well location and again
pressing READ.

When all of the samples in a strip have been read, change the strip identifier or the file
number to read the next microwell strip.

NOTE: If the well to be read is not properly aligned over the filter chamber then
pressing READ has no effect.

6. Display: The display is located in the upper lefthand corner of the Model EL301
front panel. (Refer to Section 4.3.1 for sample displays and detailed explanations
of the alphanumerics that appear within the display window.)

2-9
 ● WELL: This designator is located underneath the display window. It
shows the location within the display where the well file number is shown.

● O.D.: The optical density value that is displayed during the reading of the
wells appears at the location within the display window.

● RESULT: The calculated result (for ratio, linear equation, or difference)

appears at this location within the display window.

7. Hidden Keys: The hidden keys on the Model EL301 are located in the upper
lefthand corner of the Model EL301's front panel above the display window.
A hidden pressure sensitive key is be located at the top left of the display,
Depending on which front panel overlay revision is on the reader, another hidden key
is centered just above the display window (for the Revision C overlay) or the second
hidden key is located under the first hidden key (Revision B overlay).

● The first hidden key to the left of the display is used for a system reset similar
to the ON/OFF button (it performs a “soft boot”).

● The second hidden key (centered to the right of the first hidden key) is for the
unit’s self-testing/diagnostic routines. (When the second hidden key is
pressed immediately following pressing of the “soft boot” hidden key, the
unit identification number is displayed and then the Software Revision level
as well as the EPROM checksums.)

EL301
PART NUMBER 3010200

VERSION R03.01, C
EPROM A = 49FD

NOTE: This description applies only to units with Revision C (or greater) software.

2-10
2.2.1.1 Filter Paddle

1. Filters: The interference filters used with the Model EL301 are factory
mounted in protective cups. As depicted in Figure 2.2.1.1-1, the filter cups
are inserted into the slots in the filter paddle.

2. Filter Paddle Receptacle: The filter paddle receptacle is located on the front of
the Model EL301. The paddle is inserted into its receptacle as shown in Figure
2.2.1.1-1. When the filter paddle is in place, the filter mounted in the paddle is
aligned with the opening in the Model EL301 front panel. The light beam travels
from the optics arm, through the microwell being read, into the filter chamber,
through the filter, and strikes the photodiode.

3. Filter Paddle: The filter paddle can accommodate 2 interference filters mounted in
protective cups.

4. Indicator Lines and Stamped “W1” and “W2:” The 2 indicator lines on the
filter paddle correspond to the 2 positions of the filter paddle in the Model EL
301. These positions are for wavelengths W1 and W2. The indicator lines and
corresponding stamped “W1” and “W2” on the paddle enable the operator to see
the correct placement of the paddle in the unit; in addition, when the paddle reaches
a correct filter position, the operator is provided with tactile feedback that the
paddle is in the correct position.

2-11
Figure 2.2.1.1-1. Filter Paddle with Interference Filters

2-12
2.2.1.2 Microwell Strip Carrier

See Figure 2.2.1.2-1 for a complete illustration of the the microwell universal strip carrier.

1. Microwell Strip: The Model EL301 can be used with microwell strips with 8-
well, 12-well, or 16-well (2 x 8) configurations, as shown in Figure 2.2.1.2-1.

2. Individual Microwell Strip Insert(s): As shown in the Figure, each microwell

configuration requires a microwell strip insert that fits in the microwell strip
carrier. The microwell strips fit into their respective inserts in only one way.

3. Microwell Universal Strip Carrier: The strip carrier can be used with any of the
microwell strip configurations as long as the correct insert is used. The microwell
strip carrier is designed to slide along the raised guide rail on the Model EL301.
A magnet is embedded within the carrier that enables the Model EL301 to
automatically sense the exact well location; this information is sent to the built-in
microprocessor.

4. Grooved Surface: The carefully engineered ball-bearing detent mechanism

suspended from the Model EL301 optics arm works in conjunction with the
grooved surface on the microwell strip carrier. This mechanism ensures that each
well is properly positioned in the light beam that must travel through the microwell
being read into the filter chamber.

2-13
Figure 2.2.1.2-1. Microwell Strip Carrier

2-14
2.2.2 Rear Panel

See Figure 2.2.2-1 for the receptacles on the Model EL301 rear panel.

1. Serial Port: Located in the center of the rear panel and accessed through an
opening in the shroud of the optics arm. Note that a special cable with a snap
connector is required to connect the Model EL301 to a computer. This port
cannot be used with printers that use a serial interface. Refer to Section 3.4 for
additional information on the serial port connections.

2. Power Line Cord Receptacle: Located to the right of the serial port receptacle.
The line cord is accessed through an opening in the shroud of the optics arm.

2.2.3 Side Panels

See Figure 2.2.2-1 for the components of the Model EL301 side panels.

1. Parallel Port: Located on the left side panel when the unit is viewed from the rear.
This port requires a cable with special connectors to interface with a printer.
Refer to Section 3.3 for additional information on the parallel port configuration.

2. ON/OFF Switch: Located on the right side panel when the unit is viewed from the
rear.

2-15
Figure 2.2.2-1. Rear and Side Panel Connectors for the Model EL301

2-16
2.3 Accessories

The following accessories listed in Table 2.3-1 are provided with every Model EL301:

Table 2.3-1. Model EL301 Accessories

ACCESSORIES PART NUMBER NOTES

Filter Paddle 3010510 Holds 2 interference filters.

2 Filters 3014405 (405 nm) 405 nm and 490 nm interference filters as

3014490 (490 nm) standard filters.

Microwell Strip 3010513 (carrier) Includes inserts for 8-, 12-, and 16-well Carrier
3010515 (1 x 8) (2 x 8) strips.
3010517 (2 x 8)
3010516 (1 x 12)

Power Supply 3010506 (120 V) As requested by user for 100 V, 120 V,

3010507 (220 V) or 220 V.
3010512 (100 V)

Operator’s Manual 3011000

Warranty Card --

Certificate of -- All Bio-Tek Instruments equipment is

Calibration calibrated to meet National Bureau of
Standards (NBS) requirements.

2-17
2.4 Optional Accessories

The following optional accessories listed in Table 2.4-1 can be ordered for the Model EL301:

Table 2.4-1. Model EL301 Optional Accessories

OPTIONAL PART
ACCESSORIES NUMBER NOTES

Filter Paddle 3010510 Holds 2 interference filters.

Microwell 3010513
Strip Carrier

Strip Inserts 3010515 (1 x 8)

3010517 (2 x 8)
3010516 (1 x 12)

Battery Pack 47036 Provides up to 8 hours of power.

Cable to Connect 71066 Provides power directly from an automobile to

12 V to Power or other vehicle at remote sites.
Source

Carrying Case Inquire

Seiko Printer 97080 40-column thermal printer.

Thermal Paper 97081 For use with Seiko printer.

Lamp Assembly 3010502

Printer Cable 3011509

Serial RS-232C 3011510

Cable

2-18
 Table 2.4-1. Model EL301 Optional Accessories (Continued)

OPTIONAL PART
ACCESSORIES NUMBER NOTES

Additional Filters available in the 405 nm to 700 nm

Filters range. Other wavelengths available on
request.
3014405 (405 nm)
3014410 (410 nm)
3014415 (415 nm)

3014420 (420 nm)

3014440 (440 nm)
3014450 (450 nm)

3014457 (457 nm)

3014475 (475 nm)
3014490 (490 nm)

3014505 (505 nm)

3014510 (510 nm)
3014515 (515 nm)

3014540 (540 nm)

3014550 (550 nm)
3014560 (560 nm)

3014570 (570 nm)

3014590 (590 nm)
3014592 (592 nm)

3014595 (595 nm)

3014600 (600 nm)
3014610 (610 nm)

3014620 (620 nm)

3014630 (630 nm)
3014640 (640 nm)

3014650 (650 nm)

3014660 (660 nm)
3014690 (690 nm)

3014700 (700 nm)

2-19
2-20
 3 INSTALLATION AND CONNECTION

TO PERIPHERAL DEVICES

3.1 Unpacking and Inspection

If the shipping box is damaged, inspect the instrument for visible scratches and
dents as you unpack it. If the Model EL301 is damaged, notify the carrier and
your Bio-Tek Representative. Keep the shipping cartons and the packing
material for the carrier’s inspection.

Unpack the Model EL301 as follows:

1. Carefully open the top of the shipping box with a razor or knife.

2. Remove any packages of accessories that are found near the top of
the box.

3. Remove the protective foam end caps that enclose the Model EL301.

4. After opening the inner box, carefully remove the Model EL301
and set it on a clean, stable work surface.

5. Set the instrument in its upright position.

6. Ensure that all of the accessories and the optional accessories that
you ordered are included in your shipment.

7. Store the packing boxes and packing material in a safe place.

NOTE: Once the instrument is unpacked, all packing material must

be saved. If the packing materials are lost and the instrument needs
to be shipped, new packing material must be obtained from Bio-Tek;
otherwise your warranty may be void.

3-1
3.2 Electrical Connections

3.2.1 Wall Socket (AC Outlet)

1. Locate the power outlet on the rear of the Model EL301 (see Figure 2.2.2-1 for
exact location).

2. Plug the rounded end of the line cord into the Model EL301 and the 3-prong plug
into the wall socket as shown in Figure 3.2-1. The line power cord includes the
AC/DC converter as depicted in the figure.

CAUTION: Do not use an extension cord or cheater plug that does not have a
protective conductor. The protective earth terminal (chassis) must be connected to
the protective earth connector of the power source; this happens when the main power
cable is properly connected to the AC power source.

NOTE: For use at remote sites, the Model EL301 can be plugged into a portable,
rechargeable battery pack that provides up to 8 hours of power. In addition,
the EL301 can be connected to any 12 V source (e.g., an automobile battery).
Refer to Section 2.4 for a list of the optional accessories that can be
purchased for the microwell reader.

3. Turn ON the Model EL301 by toggling the ON/OFF switch on the left side of the
instrument.

3.2.2 Battery Pack

The battery pack provides 8 amps of power per hour and comes with its own carrying case
and charger. (Should provide 8-to-10 hours of continuous operation.)

3-2
Figure 3.2-1. Model EL301 Connections

3-3
3.3 Connecting the Model EL301 to a Printer

1. Refer to the printer operator’s manual for specific instructions to attach the printer
to the Model EL301 . Either a 40- or 80-column printer can be connected directly
to the microwell reader. (Refer to Section 2.4 for the optional printer that can be
purchased from Bio-Tek.)

2. See Figure 2.2.2-1 for the exact location of the printer port on the Model EL301 .

NOTE: The printer connection on the Model EL301 is not a standard parallel
interface and requires a custom connector.

3. See Figure 3.3-1 for a schematic of the pin assignment of the cable and connector
for the Model EL301 .

NOTE: The interface is an 8-bit parallel interface with signals consisting of: 8 data
bits, data strobe, printer busy, printer fault, and signal return. In addition, the output
drive capability is sufficient to drive an LSTTL input.

4. Refer to Section 4 for instructions on selecting the parallel printer port output
during file setup.

NOTE: The software is designed so that the Model EL301 waits 2 seconds for
the proper interface signals before displaying:

PRINTER NOT OKAY

and abandoning the requested output.

3-4
Figure 3.3-1. Schematic for Pin Assignment of Model EL301 Cable and Connector

3-5
3.4 Connecting the Model EL301 to an External Computer

1. Refer to the computer operator’s manual for specific instructions on setting up the
computer to receive data from the Model EL301 microwell reader.

2. The serial port is of DCE configuration; that is, it is wired to look like a modem.
See Figure 3.4-1 for the serial port connections.

NOTE: The interface is full duplex RS-232C serial with an RJ-24-style connector.
It transmits at 1200 baud and uses 1 start bit, 8 data bits, 2 stop bits, and a hard
ware handshake. All characters sent from the serial port are ASCII coded.

3. Use the custom cable with its snap connector to connect the Model EL301 to an
external computer for additional data manipulation. (See Figure 2.2.2-1 for the
location of the serial port on the rear panel of the Model EL301.)

NOTE: Refer to Section 2.4 to purchase the optional custom cable. Serial must be
enabled at file setup (see Figure 4.3.1-1).

3.5 Installing Interference Filters in the Filter Paddle

1. See Figure 2.2.1.1-1 for a detailed illustration of the interference filters and the
filter paddle for the Model EL301 .

NOTE: The filters are factory-mounted in protective filter cups that fit securely
into the openings in the filter paddle.

2. Refer to Section 2.4 to order additional filters (and filter paddles, if desired).

3. Remove the filters in the paddle by gently tapping the filter paddle into the open
palm of your hand; the filters should be released from the openings in the filter
paddle.

NOTE: Never force the filters out of the paddle with a sharp object as this can
scratch and permanently damage the filters.

4. Gently press the new filters into the openings on the filter paddle. Take care not to
touch the filters themselves because fingerprints cause the optical density readings
to be inaccurate.

3-6
Figure 3.4-1. Serial Port Connections for the Model EL301

3-7
3-8
 4 OPERATION

4.1 Introduction

The Model EL301Strip Reader is controlled from the reader’s front panel. This
section describes how to operate the instrument including how to set up files as
well as how to read the microwell strips.

Even though the Model EL301is a portable reader, it is designed with many
features and operation at first may appear quite complex. Read this section
carefully and go through all of the steps in Section 4.4.4 (Setting Up a File:
Tutorial) to ensure that you fully understand the many features that this reader
can provide.

4.2 Preliminary Procedures

Before turning on the Model EL301, confirm the following:

1. The reader has been installed according to the instructions in

Section 3.

● All electrical cables are properly connected and grounded (refer to Section 3.1
for details).

● All connections between the Model EL301and all peripheral devices are
secure as described in Sections 3.3 and 3.4.

2. The microwell strips to be used are clean and dust-free.

3. The interference filter lenses in the filter paddle are clean (refer to
Section 5.2.3) and the working surface and the non-skid rubber feet
on the bottom of the instrument are clean (refer to Section 5.2.2).

CAUTION

Operation of the Model EL301in direct sunlight is not recommended

because sunlight has all light frequencies and it can interfere with the
optical density readings.

4-1
4.3 Definitions

The following sections describe all of the operational functions of the Model EL301. If you
are using this instrument for the first time, be sure to read Section 2.2.1 carefully to learn the
front panel locations and definitions of the buttons on the Model EL301 .

4.3.1 Display Windows on the Model EL301

See Figure 4.3.1-1 for a complete description of the alphanumerics that appear in the LCD
window during a reading, or when the Data Display Mode is accessed (by pressing the
DISPLAY/SET-UP button).

When the power to the Model EL301is turned ON, the instrument defaults to the Data
Display window shown in Figure 4.3.1-1c. Pressing the DISPLAY/SET-UP button
toggles the display between the Set-Up Display and the Data Display.

4.3.1.1 User Prompts

The Model EL301communicates with the operator by means of user prompts. The prompts
ensure that the file parameters and options are entered where required and that data entry
steps are followed in the proper sequence. For example:

1. When blanking a well or when reading in dual wavelength, the display

prompts the operator to enter the data in a specific order: This minimizes
the use of inaccurate data or the accidental erasure of stored data.

2. When in the submenus of file setup, pressing the DISPLAY/SET-UP button

does not switch to the Data Display screen; instead, a COMPLETE SETUP
message is displayed. This forces the operator to step through each prompt
of a particular submenu and to fix any setup errors before exiting to the Data
Display Mode.

4-2
 WELL O.D. RESULT

where:

WELL = well file number

O.D. = optical density
RESULT = calculated result for ratio, linear equation, difference,
or limit call (+, -, or +/-)

a. Model EL301Display Window

F1 READY/MC 1A
A1 0.542b -1.234

where:

F1 = file number
READY = current status (3 options READY = ready to read well, W1 = reading
at filter wavelength W1, W2 = reading at filter wavelength W2)
MC = memory flag (MC = memory clear, MF = memory full)
1A = memory area designator
A1 = well location
0.542 = optical density (can be negative if O.D. of well is less than
O.D. of blank well)
b = well type (b = blank, s = standard, no letter = sample well)

b. Display Window Appearance During Reading

Figure 4.3.1-1. Display Windows Defined for the Model EL301

4-3
 F1 SETUP L S P
8 D ABSORB Y N Y

where:

F1 = file number
SETUP = setup mode header
L = limits set header
S = serial output header
P = parallel output header
8 = strip configuration (options = 8, 12, or 16)
_ = cursor (moves when PROMPT pressed)
D = wavelength mode (D = dual, S = single)
ABSORB = calculation type (ABSORB = absorbance, RATIO = ratio,
LINEQU = linear equation, DIFF = difference)
Y = limits set (Y = yes, N = no)
N = serial output (Y = yes, N = no)
Y = parallel output (Y = yes, N = no)

c. Display Window Appearance during Data Set-Up Mode

Figure 4.3.1-1. Display Windows Defined for the Model EL301(Continued)

4.3.1.2 Error Messages

User error messages inform the operator when procedural errors are made; for example:

1. When the display shows READY/MC it means that a particular strip in a particular
file is ready to be read; that is, the memory is clear. After the strip is read, the MC
changes to MF, memory full. If the operator forgets to change the well location or
strip designator, then the Model EL301will display the error message:

CLEAR MEMORY
TO REREAD STRIP

This prevents the operator from accidentally reading over data stored in memory.

4-4
2. During the Set-Up Mode, if the operator attempts to enter the parameters in the
incorrect order, an error message will appear on the screen to inform the operator of
the problem.

3. If the DATA OUT button is pressed when the printer is enabled but the printer is not
hooked up or is not on-line, the Model EL301will display the error message:

PRINTER NOT OKAY

NOTE: If DATA OUT is pressed and the external computer is not turned on or con-
nected to the reader’s serial port, the display will continually show:

SENDING DATA

There is no way to resume normal reader functions from this condition except to turn the
reader OFF and then ON again.

4. If CLEAR is pressed, the operator can switch from the Display Mode to the Set-Up
Mode by pressing the DISPLAY/SETUP button.

5. Remember that after reading a strip, the operator must prompt for the specific file
location when switching from the Display Mode to the Set-Up Mode. If the specific
file location is bypassed, the Model EL301emits an audible double tone.

6. During initial filter calibration at power up, the message on the display instructs the
operator to:

REMOVE CARRIER
THEN HIT ENTER

The reader senses the actual presence of the carrier and if ENTER is pressed but the
carrier is still in place, the reader will beep twice and the display will remain the same.

4-5
7. If single wavelength has been selected during the file setup but the W1/W2 button is
pressed during the reading function, the reader will beep twice and the following
display will alert the operator to this inconsistency:

F1 W1/W2 1a
CHECK SETUP

4.3.2 Files

The Model EL301has 4 setup files: F1, F2, F3, and F4. Each file can be set up to define a
different set of parameters (or modes) that determine which types of data are obtained. (For
example, the file could be set up to read the strip using dual wavelength, a 12-well configura-
tion, and to read absorbances only.) Each of these files functions independently of all other
files and indicates to the operator where the data are stored in the non-volatile RAM of the
Model EL301 .

Within each file, the individual strips are labeled with a letter and a number. It is possible to
have 3 memory locations of A, B, and C. These memory locations are further identified as 1
of 6 strips so that the strip locations are designated as: 1A, 2A,...6A, 1B,...6B, and 1C,...6C,
thus giving a total of 18 possible strips within a single file. For example, a strip could be in
file F3 with a memory location of B2. Each strip contains readings for up to 16 (2 x 8 con-
figuration) microwells.

NOTE: The dual wavelength 8-well strip is considered a single strip. When all possible
microwell locations are multiplied together, the total number of microwell readings that can
be stored in the Model EL301memory equals 1,152 (or 12 full 96-well microplates). See
Figure 4.3.2-1 for a graphic representation of how the file numbers, memory locations, and
associated microwell strips are accounted for in the calculations. It is clear from the figure
that different files can have the same memory locations (e.g., location A3 of F1, location A3
of F4).

NOTE: Blanking values, standards, and ratios are maintained from strip to strip within the
same file.

4-6
4.3.2.1 Calculation Formats Available

There are 4 data reduction functions that can be chosen for the wells being read (refer to
Section 4.3.3.4 for a complete definition of the data reduction that the Model EL301can
perform):

1. 8-Well configuration: absorbance, ratio, linear equation, and limit.

2. 12-Well configuration: absorbance, ratio, linear equation, and limit.

3. 16-Well configuration: absorbance, ratio, linear equation, difference, and limit.

Only the 16-well (2 x 8) configuration gives a difference value.

There are 6 characters reserved on the LCD for the calculation results. The display can show:

1. Concentration values of a 4-digit number with 1 digit to the left of the decimal
point (e.g., 123.4, a positive value; -123.4, a negative value).

2. “+.+++” for overrange values (> 2.999).

3. “-.---” for underrange values (< -2.999).

4. “+,” “-,” or “+/-” if limits were set for the readings (e.g., if the upper limit were
2 and the lower limit were 1, then “+” would be displayed for values > 2, +/- for
values > 1 and < 2, and “-” for values < 1).

NOTE: Remember that LIMIT1 is the lower limit and LIMIT2 the upper limit;
therefore:

Absorbance > LIMIT2 “+”

LIMIT1 < Absorbance < LIMIT2“+/-”
Absorbance < LIMIT “-”

4-7
Each File defines Data Reduction Program
and the Location of Stored Data.

F1 F2 F3 F4

Each File contains 3

Plates, (A, B, C)
where strips are
read and data
stored.

A B C

Each Plate contains 6

Strips, (1, 2, 3,
4, 5, or 6)
where wells are
read and data
stored.
1 2 3 4 5 6

Each Strip contains

8, 12, or
16 Wells.
8 12 16

Figure 4.3.2-1. Number of Wells Stored in Memory

4-8
4.3.3 Functions Performed by the Model EL301

The portable Model EL301Strip Reader is designed to function in much the same way as
larger, stationary readers. The following sections clearly define all of the operating functions
of the Model EL301. When you have completed reading the sections explaining these func-
tions, then you are ready to follow the tutorial that takes you through all setup and operating
steps of the portable reader. Section 2.2.1 describes the buttons on the Model EL301front
panel. Some of the definitions contained in Section 2.2.1 are included in the following text to
provide continuity within this section as you are learning about the strip reader.

The operating functions performed by the Model EL301include:

1. Creating blanking wells and creating standards for ratio and linear equation forms of
data reduction (refer to Section 4.3.3.1).

2. Clearing (refer to Section 4.3.3.2).

3. Reading with single or dual wavelengths, with or without setting limits for
absorbance and concentration values (refer to Section 4.3.3.3).

4. Performing data reduction on the data; the data reduction forms include: absorbance,
ratio, linear equation, and difference calculations (refer to Section 4.3.3.4).

5. Transmitting data through the parallel and serial ports to a printer and external
computer, respectively, to obtain hard copies of the data or for further data
manipulation (refer to Section 4.3.3.5).

NOTE: The data is transmitted via the serial interface in ASCII code.

4.3.3.1 Blanking and Standards

4.3.3.1.1 Blanking

The BLANK button is located to the right of the DATA buttons on the Model EL301front
panel. It is used to instruct the microprocessor to read the current microwell aligned with the
filter chamber as a blank. When the Data Display window is shown on the LCD, this button
enables the operator to read the optical density of the microwell aligned over the filter cham-
ber. This value is used as the blanking value that is subtracted from all other microwell
readings in that file until a different blanking value is entered. If the microwell carrier is not
properly aligned over the filter chamber, pressing BLANK has no effect and the carrier should
be realigned to give a valid well location.

4-9
NOTE: If there is no microwell carrier in place when BLANK is pressed, that well location is
blanked against air. Be sure to remove the microstrip carrier so that the reader does not blank
on the black carrier. If the strip has been cleared and then blanking is initiated on that strip,
then the blanking value is retained and the operator is not forced to re-blank unless he or she
wishes to re-blank on a particular well.

When a well is successfully blanked, the Model EL301emits an audible tone when BLANK is
pressed. A blanked well shows up on the display or on the printout as the optical density
value immediately followed by the lowercase letter “b.” Blanks can be carried over from
strip-to-strip within the same file by simply continuing to read the sample wells. A new blank
can replace the current blank if BLANK is pressed before beginning to read a new microwell
strip. The new blanking value is subtracted from all subsequent readings.

From the previous paragraphs, it is apparent that when the operator begins a new microplate
reading sequence, the Model EL301will automatically retain the blanking values. These
values will be carried over into the reading of subsequent strips unless one of the following
conditions arises:

1. Operator returns to SETUP Display to change filter parameter from Single to

Dual or Dual to Single: If this situation occurs, then all blank locations are retained
only for the samples already read; these values are cleared from memory as the
blanking locations. The Model EL301forces the operator to read in the new blank(s)
value(s) at the point in the prompting sequence where he or she changed to a new
wavelength setup.

If the operator changes from Single to Dual wavelength, then the Model EL301will
prompt for both filter wavelength readings; if the operator changes from Dual to
Single, then the EL301will prompt for just one filter wavelength reading.

2. Operator presses CLEAR key during reading of a Blank or reading of a

Sample: When CLEAR is pressed, all Samples and Blanks are deleted from memory
and theModel EL301prompts the operator to enter new blanks for the microwell
strip. If no Blanks have been read in the strip, only the Sample wells will be cleared
from memory but any Blanks already retained for the preceding strips which com-
prise the microplate will be retained and used to blank against the new Sample well
OD values.

3. Operator presses the BLANK key on a new microwell strip: When this occurs,
the Model EL301ignores any blanks already programmed into the microplate SETUP
for all subsequent strips to be read. The Model EL301will accept the new Blanks
entered by the operator at the current strip and use them for the subsequent readings.

4-10
Remember to observe the following protocol when using the blanking function of the
Model EL301 :

1. When the Model EL301is turned ON or after a blank has been deleted by pressing
the CLEAR button, a new blanking well must be entered before beginning to read the
microwell strip. An attempt to read the strip will cause the unit to display the error
message:

NO BLANKING YET

2. Blanks must be read first if not already established for that particular file setup.
When BLANK is pressed, the READY/MC on the display changes to the first wave
length to be read, W1, so that the display shows W1/MC.

3. Consecutive blanks are created by pressing BLANK for the first well in the strip and
then sliding the microwell carrier to the next valid well location and pressing
BLANK again. This process can be repeated for as many consecutive blanks as
desired in that strip.

NOTE: The consecutive blank well values are averaged together and it is this
average value that is subtracted from all sample wells; however, one well must be
read to trigger the blank-averaging routine.

4. Blanks cannot be read within the strip after the first sample is read.

5. Standard wells (if defined in the file setup) must be entered immediately following
the blanking well but before any sample wells are read. (Refer to the following
Section 4.3.3.1.2 for the description of standard wells.)

6. Blanks and standards entered for the first wavelength, W1, of a dual wavelength
reading must also be entered on the same well location during the second wavelength,
W2, reading. (As demonstrated in the tutorial, Section 4.4.4, the Model EL301
prompts the operator for these blanks and standards in the proper sequence and
displays error messages if this sequence is not followed.)

NOTE: If a previously used file is not cleared completely (with the exception of
Strip 1), the blanking function will continue even if CLEAR is pressed.

4-11
4.3.3.1.2 Standards

When LINEQU or RATIO is selected as the calculation option for data reduction during file
setup, standard wells must be entered as a file parameter. (Refer to Section 4.3.3.4 for a
complete description of the calculation options for data reduction.)

Remember to use the following protocol when setting standards for a Model EL301file:

1. During file setup, decide which well(s) should be used as the standard(s).

NOTE: Standard wells can only exist on the first strip of a 6-strip group. (Picture the
microwell strips combined together to form a full 96-well microplate.) The standards
designated for this first strip can be used for all strips within this 96-well microplate
equivalent.

2. The standards designated in Step 1, preceding, can be used for other microplate
equivalents within the same file; that is, for A, B, or C. The carryover of these
standards is optional. The display shows the following prompt after the blank is read:

NEW STANDARDS?
OPTION OR ENTER

Pressing the OPTION button changes the display to:

SAME STANDARDS?
OPTION OR ENTER

At this point, the operator can decide to retain or change the designated standards by
pressing ENTER when the desired prompt appears on the display.

NOTE: When a file is cleared (by pressing CLEAR) and re-read, the reader queries the
operator for NEW or SAME STANDARDS?

4-12
 3. Standard wells (as designated in the file setup) must be read immediately
following the reading of blanking wells (described in Section 4.3.3.1, preceding)
but before the sample wells are read.

NOTE: If the operator forgets that a standard has been designated and presses
READ after the blanking well is entered, the Model EL301emits an audible tone
and dis plays the following error message for 3 seconds:

F1 W1/MF 1A
READ STANDARDS

4. Standards and blanks entered for the first wavelength, W1, of a dual wavelength
reading must also be entered on the same well locations during the second wave
length, W2, reading. The Model EL301prompts the operator for these standards
and blanks during file setup.

4.3.3.2 Clearing

Data is saved in the Model EL301non-volatile RAM even if the power is removed. The
CLEAR button clears digits in the numerical entry prompts. This clearing action takes place
when entries are made for the concentration values used for RATIO and LINEQU calcula-
tions and when limit values are chosen.

Remember to observe the following protocol when using the clearing function of the reader:

1. During the file setup, pressing CLEAR clears the bottom line of the display and
returns the prompt to the last chosen parameter. This enables the operator to re-enter
data for concentrations and limits.

2. During the Data Display Mode, pressing CLEAR clears the data stored in either the
file, the plate, or the strip, depending on the location of the cursor when CLEAR is
pressed. The parameters associated with the memory location of the setup file are not
cleared when the data entries are removed. The cursor must be placed under A, B, or
C if the entire memory location is to be cleared.

NOTE: Place the cursor under the data memory location to be cleared before press-
ing CLEAR. (An entire file can be cleared instead of just a strip if the cursor is incor-
rectly placed under the file designator instead of the strip designator.)

4-13
3. Remember to use CLEAR to clear any blanks, standards, or differences that are not to
be carried over to the new file to be read. (Refer to Section 2.2.1 for additional
information.)

4.3.3.3 Reading

The READ button is located in the upper lefthand corner of the Model EL301front panel.
When the microwell strip carrier is moved from well to well, the Data Display window shows
either the previous reading for the well aligned with the filter chamber or “_._ _ _” if there is
no previous reading. The Model EL301emits an audible tone when the well has been read
successfully. The reading appears in the display window such that the well location appears
over WELL, the optical density reading appears over O.D., and any calculated results for
ratio, linear equation, or difference appear over RESULT.

Some wells read by the Model EL301will have Absorbance values that are designated as
OVERRANGE values (i.e, with OD’s > 2.999) or UNDERRANGE (i.e., with OD’s
< -2.999). (In the following description, only OVERRANGE will be discussed but the same
applies to the UNDERRANGE values that are represented on the printout as: -.---.) These
values are treated differently depending on the parameter Setup. In all cases, however, the
OVERRANGE OD values are considered to be invalid test data. The following list
describes how OVERRANGE values are treated in different situations:

1. During reading of Dual Wavelength: If either of the 2 filter readings on a given

Sample are detected to be OVERRANGE OD values, then the final Absorbance
result is invalidated. Any subsequent calculations performed are also invalidated.
The Model EL301display warns the operator that an OVERRANGE condition exists
andthe OD appears on the printout as: +.+++. Any values intended for the Results
Column on the printout appear as: ?.??? for the OVERRANGE well.

2. Standard well is detected as OVERRANGE: If this occurs, then all Standards for
that microwell strip are considered invalid. The operator is warned that the STD well
is OVERRANGE in the display window and the OD appears on the printout as:
+.+++ and all results for each Standard and Sample well read are printed as: ?.???.

3. During Difference or Ratio calculations: For these calculations, any

OVERRANGE values will invalidate the OD value and the calculation result for the
OVERRANGE well(s). The LCD display will show a warning message, OD
OVERRANGE, and the printout will show: +.+++ and ?.??? for the Absorbance
value and result, respectively.

4-14
 4. Blank is detected as OVERRANGE: If the Blank is OVERRANGE, it is ignored
and the Model EL301prompts the operator to re-blank until the unit has been blanked
on a valid well. If the invalid Blank is one of a number of blanks that are averaged
together to give a final blanking Mean, then the invalid Blank value is not used in the
averaging process. The Model EL301display will warn the operator that the well is
OVERRANGE and the printout will show the Blank well (“b”) Absorbance as:
+.+++ and the calculated result as: ?.??? (if any results were expected based on the
parameter Setup).

5. Sample well is detected as OVERRANGE: If this occurs, then the Model EL301
display warns the operator that the OD is OVERRANGE and the display shows the
value as: +.+++. The OVERRANGE values appear on the printed as: +.+++
and ?.??? for the Absorbance value and result, respectively.

Remember to use the following protocol when using the reading function of the Model
EL301:

1. Ensure that all blanks (Section 4.3.3.1.1) and standards (Section 4.3.3.1.2)
designated in the file setup are entered before trying to read the optical density of a
sample well.

NOTE: Pressing READ causes the Model EL301to read the optical density of the
well aligned over the filter chamber and then to subtract any blanking values from the
optical density reading; it is this difference that is displayed on the LCD.

2. The wells are read by sliding the microwell carrier on the raised guide rail on the
front panel (refer to Section 4.4.2). When the well is properly aligned over the filter
chamber as indicated by a new well location on the display, pressing READ will read
the new well.

NOTE: If the well is not aligned over the filter chamber, pressing READ has no
effect.

3. When all of the samples in the strip have been read, change the strip identifier or the
file number to read the next microwell strip.

4. The same well can be read repeatedly by continually pressing READ; however, only
the most current reading is retained in memory.

5. The CLEAR button can be used at any time during the reading of the wells (refer to
Section 4.3.3.2 for a complete description of the clearing function).

4-15
 The actual reading of the microwells is determined by the following user-defined
conditions:

1. Reading at single or dual wavelength (refer to Section 4.3.3.3.1).

2. Setting limits for absorbance and concentration values (refer to Section 4.3.3.3.2).

4.3.3.3.1 Single Wavelength versus Dual Wavelength

The Model EL301can measure optical densities by reading the microwell at a single wave-
length or by reading the microwell twice, first at the filter wavelength, W1, and then at the
filter wavelength, W2.

Remember to observe the following protocol when reading at single or dual wavelengths:

1. Both filters are calibrated after power up. It is important that the instructions shown
on the reader display are followed exactly.

2. Select “S” for single and “D” for dual wavelength during the file setup.

3. The W1/W2 button toggles between the interference filter wavelengths W1 and W2
only for dual wavelength designations on the Data Display window.

NOTE: Filter W1 must be used for readings before filter W2 can be used.

4. Calibration is performed on both filters when the Model EL301is turned ON.

NOTE: The reader must be turned OFF and then ON to initiate the recalibration
process when switching from double-to-single wavelength or from one single wave-
length to another single wavelength.

5. The Model EL301software ensures that the microwell strip carrier is removed before
calibration by displaying a user prompt on the LCD. The software cannot, however,
detect if a filter is actually mounted in the filter paddle or which filter is mounted in
the paddle.

NOTE: The operator must ensure that the proper filter is installed in the proper filter
paddle position during the calibration procedure.

4-16
6. Press W1/W2 to toggle between the filters mounted in the filter paddle. When
reading using dual wavelengths, the absorbance values for all of the wells are initially
stored but then they are replaced by the difference W1 - W2 as soon as the readings at
W2 are taken. No values are shown on the display until the W2 readings are taken.

NOTE: It is not possible to obtain the individual values for W1 or W2 without re-
reading the strip in the single wavelength setting.

7. If single wavelength is selected during file setup but the W1/W2 button is pressed
during the reading function, the Model EL301will emit an audible beep and display:

F1 W1/MC 1A
CHECK SETUP

8. If single wavelength measurements at the W2 wavelength are desired, switch OFF the
unit, change filters, and recalibrate.

4-17
4.3.3.3.2 Setting Limits for Absorbance and Concentration Values

An upper and a lower limit can be selected for the data reduction calculations. During the file
setup, the operator can choose to use limits by toggling the OPTION button to “Y” for yes
when the cursor is under the “L” on the setup display. When the yes option is entered, the
Model EL301 first prompts for the lower limit, LIMIT1, and then the upper limit, LIMIT2.
Each of these limits defines a threshold (or cut-off) value that is interpreted by the micropro-
cessor.

Remember to observe the following protocol when using limits during data reduction calcula-
tions:

1. The values for the limits must be in the same measurement units as the calculation option
that is selected.

Calculation Type Limit Units

ABSORBANCE Absorbance
RATIO Concentration
LINEQU (Linear Equation) Concentration
DIFF (Difference) Absorbance

2. When the calculated data appear on the display or the printout, the following symbols
are used:

“+” For values greater than the upper limit (Abs.> LIMIT2).
“+/-” For values between the upper and lower limits
(LIMIT1 < Abs. < LIMIT 2).
“-” For values less than the lower limit (Abs. < LIMIT1).

NOTE: The lower limit is always LIMIT1 and it is always entered first in the prompting
sequence.

3. Within a given file number, the following combinations of calculation options may be
used:

ABSORBANCE/RATIO ABSORBANCE, RATIO, LIMIT

ABSORBANCE/LINEQU ABSORBANCE, LINEQU, LIMIT
ABSORBANCE/DIFF ABSORBANCE, DIFF, LIMIT
ABSORBANCE/LIMIT

4-18
 NOTE: Single or dual wavelength readings can be taken for any of the preceding combi-
nations of options.

4. The concentration limits are entered and displayed as: “_ _ _ . _” and the optical
density (absorbance) limits are entered and displayed as:
“_ . _ _ _” (e.g., 123.4 and 1.234, respectively).

5. When the calculation option is changed in the file setup, the limits option is
automatically changed to “N” (no). This is intended to prompt the operator to re-
enter the limits for the new calculation option.

4.3.3.4 Data Reduction

There are 4 kinds of data reduction that can be selected on the Model EL301:

1. ABSORB (Absorbance): Used to read, display, and print only the optical density
values of the microwell strips (refer to Section 4.3.3.4.1).

2. RATIO: Used to read, display, and print the absorbance values and then to calculate
a concentration value by creating ratios between the sample wells and a known stan-
dard well (refer to Section 4.3.3.4.2).

3. LINEQU (Linear Equation): Used to read, display, and print calculated

concentration values of sample wells. These calculations are based on extrapolation
from the curve generated by plotting the observed optical densities against the known
concentrations of from 2 to 5 standard wells (refer to Section 4.3.3.4.3).

4. DIFF (Difference): Used to read, display, and print the calculated difference
between the optical densities of the 16-well configuration (2 x 8); that is, the value of
A1 - A2 (refer to Section 4.3.3.4.4).

4.3.3.4.1 Absorbance

Remember to observe the following protocol when using the ABSORB function:

1. Select a blank well (or group of blank wells) whose value will be subtracted from all
sample readings (refer to Section 4.3.3.1.1 for a description of blanking).

2. Select single or dual wavelength with which to read the microwell strips.

4-19
 3. Set limit values (if desired) for the optical densities (refer to Section 4.3.3.3.2 for
setting limit values).

4. Remember that the calculation type is reset to ABSORB when the strip configuration
is changed from the 16-well to 8-well configuration. This prevents the carryover of
invalid standard wells.

4.3.3.4.2 Ratio

This method of data reduction is used to calculate the concentration of the sample wells. The
operator enters the known concentration of a standard well, reads the optical density of that
well, sets up a ratio of the known concentration to the measured optical density of the stan-
dard well, and uses this value to calculate the concentration of the sample wells when only the
optical densities are measured. Mathematically this is expressed by the equation:

ConcentrationStandard = ConcentrationSample
__________________ __________________

AbsorbanceStandard AbsorbanceSample

For example, with a CONCENTRATION of the known STANDARD is 200 UG/ML and
reader-determined ABSORBANCE of 0.200, the CONCENTRATION of the SAMPLE can
be back-calculated by measuring the ABSORBANCE of the sample well and using the pre-
ceding equation such that:

200 UG/ML = “x” UG/ML

__________ __________

0.200 Abs. 0.100 Abs.

“x” = 100 UG/ML

This is the calculated SAMPLE CONCENTRATION.

4-20
Remember to observe the following protocol when using the RATIO function:

1. The output of the ratio calculation consists of a number from 000.0 to 999.9.

2. The operator can choose concentration units of nanograms per milliliter (NM/ML),
micrograms per milliliter (UG/ML), or can choose no units (NONE).

3. The operator can choose limits for the concentration values (refer to Section
4.3.3.3.2).

4. The operator can choose to blank against air, use one blank well, or a group of blank
wells. Any blanks are subtracted from the standard well as well as the sample wells
(refer to Section 4.3.3.1.1).

5. The blank wells must be read first immediately followed by the standard well or
wells (refer to Section 4.3.3.1.2).

NOTE: A standard well cannot also be used as a blank well.

6. RATIO and LINEQU cannot be used in the same file (they are mutually exclusive).

4-21
4.3.3.4.3 Linear Equation

The linear equation form of data reduction enables the operator to obtain the concentration
values for sample wells by reading the optical densities of these wells. As shown in Figure
4.3.3.4.3-1, the microprocessor in the Model EL301uses the classic point/slope equation to
calculate the sample concentration values. The point/slope equation is as follows:

y = mx + b

where:

y = measured optical density of the sample well (shown on y-axis)

m = the slope of the best-approximation line drawn through the points by the 2 to 5
standard wells of known concentration and measured optical densities

x = calculated concentration of the sample well (this value is extrapolated from the
measured optical density of the sample, shown on the x-axis)

b = the y-intercept of the line (as drawn on the figure, the y-intercept would be zero).

The operator must provide the concentrations for 2 to 5 standard wells and read the optical
densities of the wells to create the line used in the linear equation form of data reduction.

Remember to observe the following protocol when using the LINEQU function:

1. All blank wells must be read first (refer to Section 4.3.3.1.1).

NOTE: Only the first standard well can also be used as a blank.

2. All standard wells (from 2 to 5) must be read immediately following the blanking
well (refer to Section 4.3.3.1.2).

3. Standard wells must be entered sequentially and only the first location will be
defined.

NOTE: All standards must appear on the first strip of the microplate (consisting
of strips treated as a full 96-well microplate).

4. Limits can be set for the concentration values (refer to Section 4.3.3.3.2).

4-22
5. The standard well location for LINEQU is reset to the first well of the strip when
the number of standards is changed in the file setup. (This is done to prevent
invalid well locations from carrying over to the new setup.)

6. RATIO and LINEQU cannot be used in the same file setup; they are mutually
exclusive.

Figure 4.3.3.4.3-1. Graphic Interpretation of Linear Equation

4-23
4.3.3.4.4 Difference

The DIFF form of data reduction is used only with the 16-well (2 x 8) configuration. The
Model EL301microprocessor calculates the difference by subtracting the value of the optical
density of a well in the first strip of the 2 x 8 strip from the corresponding well in the second
strip; that is, A2 - A1, B1 - B2.

Remember to observe the following protocol when using the DIFF function:

1. Enter blanks first as described in Section 4.3.3.1.1.

2. Enter limit values (if desired) as described in Section 4.3.3.3.2.

3. Note that only the calculated difference is displayed or printed out with the
ABSORBANCES.

NOTE: If the LIMITS option is selected, only the calls “+,” “+/-,” and “-” are printed
and if LIMIT1 = LIMIT2, the only “+” and “-” are printed.

4.3.3.5 Data Output to Peripheral Devices

The DATA OUT button transmits data out of the parallel printer port and the RS-232C serial
port depending on the options selected during file setup.

Remember to observe the following protocol when using the data output function:

1. During file setup, select the options to transmit data out of the ports on the Model
EL301. (Refer to Section 3.3 for transmission to the printer and Section 3.4 for
transmission for the external computer.)

2. Data can also be transmitted out the ports by locating the cursor under the File
Number or the Memory Location on the Data Display and pressing the DATA OUT
button. The following message appears on the display:

SENDING DATA

3. If DATA OUT is pressed during data transmission, the transmission will be

terminated.

4. When the Data Set-Up display appears, pressing DATA OUT causes the file setup to
be printed.

4-24
 5. If DATA OUT is pressed and the printer is not hooked up to the Model EL301, an
error message appears on the display to inform the operator that data transmission
was unsuccessful:

PRINTER NOT OKAY

6. If DATA OUT is pressed and the computer is not hooked up to the serial port (or is
not turned on), the reader will try to transmit data. The reader will hang up at this
point.

NOTE: The only way to resume operation of the reader is to turn the reader OFF and
then ON again. The operator must ensure that the setting for the serial port in the Set-
Up Display (“Y” or “N” underneath the “S”) corresponds to the setup being used.

7. The printout is limited to a width of 40 characters.

8. For the dual wavelength mode, well data are only reported if the wells have been read
and blanked in both wavelengths W1 and W2.

4.4 Operation

4.4.1 Power ON

The Model EL301uses a non-volatile RAM (NVRAM); therefore, when the reader is turned
ON, all user data storage and file setup information is retained.

1. Refer to Section 2.2.1 for the location of the ON/OFF switch on the Model EL301.

2. Refer to Section 3 for a complete description of electrical safety and how to connect
the Model EL301to peripheral devices.

3. Turn ON the Model EL301and then turn ON any peripheral devices connected to the
reader.

NOTE: Ensure that the printer (if used) is on-line and ready to receive data. Enable
the serial port only if you intend to connect the reader to a computer.

4. Refer to the following Sections 4.4.2 and 4.4.3 for instructions on using the
microwell strip carrier and the filter paddle, respectively.

4-25
4.4.2 Positioning the Microwell Carrier on the Model EL301Guide Rail

1. Refer to Section 2.2.1.2 for a complete description of the components that comprise
the microwell strip carrier.

2. See Figure 4.4.2-1 that shows how the carrier slides along the raised guide rail on the
front panel of the reader. The figure also shows that bottom view of the carrier.

NOTE: There are 2 grooves on the bottom of the carrier; these are used when the 16-
well (2 x 8) configuration is used. The groove closest to the carrier edge positions the
microwell strip furthest from the opposing edge over the filter chamber.

3. Observe the detent mechanism that is suspended from the optics arm that works in
conjunction with the grooved surface on the microwell strip carrier. This mechanism
ensures that each well being read is aligned over the filter chamber.

4.4.3 Inserting the Filter Paddle into the Model EL301

1. Refer to Section 2.2.1.1 for a complete description of the filter paddle components.

2. Refer to Section 3.5 if you wish to change the interference filters mounted in the
filter paddle.

3. Ensure that the proper filters are placed in the appropriate openings in the filter
paddle. (Filter W1 is placed in the opening that enters the paddle receptacle first as you
insert the paddle.)

4. Use the indicator lines on the filter paddle for visual verification that the proper filter
is in the filter chamber.

4-26
Figure 4.4.2-1. Positioning the Microwell Strip Carrier on the Model EL301
Guide Rail

4-27
4.4.4 Setting Up a File: Tutorial

This section is designed to go through every step in setting up a file on the Model EL301 .
Operation of the Model EL301is described in a stepwise manner. This section has been
subdivided into identifiable functions to enable the user to locate the specific steps required to
perform a particular function.

Perform all of the operational procedures described in Sections 4.4.4.1 through 4.4.4.6 to set
up the file parameters and then perform the procedures in Section 4.4.5 to actually read a
microwell strip.

4.4.4.1 Power Up Setup and Calibration

1. Turn ON the power to the Model EL301 . The display will show:

+::+::+::+::+::+::+::+::+::+::+::+::+::+::+::+:
+::+::+::+::+::+::+::+::+::+::+::+::+::+::+::+:

for approximately 3 seconds.

2. When the Model EL301is turned ON, the display will show:

REMOVE CARRIER
THEN PRESS ENTER

Manually remove the carrier; the display will show:

INSERT FILTER W2
THEN HIT ENTER

3. Insert the filters into their protective cups in the filter paddle (refer to Section 3.5 for
filter installation procedures).

NOTE: The operator must ensure that the filters are mounted in the correct slots in
the filter paddle (observe the indicator lines and the stamped W1 and W2 on the filter
paddle). In addition, the reader cannot determine if the filter paddle is properly in-
serted into the filter paddle receptacle; again, this is the responsibility of the
operator.

4-28
 4. Press ENTER; for approximately 2 seconds the display will show:

CALIBRATING
WAVELENGTH W2

When calibration is complete for that filter, the display will show:

INSERT FILTER W1
THEN PRESS ENTER

Push the filter paddle into the W1 position and then press ENTER; for approximately
2 seconds the display will show:

CALIBRATING
WAVELENGTH W1

5. When the reader has calibrated for the second filter, the reader is ready to be used
read microwell strips. The following display will appear:

F1 READY/MC 1A
___

6. If FILE 1 indicated in the preceding display is set up as desired begin reading; if the
setup must be changed, proceed to Section 4.4.4.2 to change the file number and
memory location.

4.4.4.2 File Number and Memory Location Setup and Flowchart

1. The display will show:

F1 READY/MC 1A
___

4-29
 NOTE: The cursor is positioned under the Memory Area Designator. (Refer to
Section 4.3.1 for definitions of the display windows.) When the preceding display ap-
pears, there is no carrier in place and the memory is clear as designated by MC; if the
memory were full, MF would appear in place of MC.

2. Press OPTION repeatedly to change the Memory Area Designator; that is, 1A, 2A,
3A, 4A, 5A, or 6A.

NOTE: The cursor remains under the numbers which change. When all of the op-
tions have been cycled through the display wraps around and returns to the first
option, 1A.

3. When the display shows the memory location number desired, press PROMPT to
advance to the next parameter setting; the display will show:

F1 READY/MC 1A
___

NOTE: The cursor has moved under the letter of the Memory Area Designator.

4. Press OPTION to change the letter designator from A to B to C and back to A.

5. When the display shows the memory location letter desired, press PROMPT to
advance to the next parameter setting; the display will show:

F1 READY/MC 1A
___

NOTE: The cursor has moved under the number of the Memory File Number.

6. Press OPTION to cycle through the Memory File Number options: F1, F2, F3, and
F4.

NOTE: As you cycle through the Memory File Locations the display differ from that
shown in Step 5, preceding, because the various files may have been set up previously
or they may actually contain data from readings that have not been cleared.

7. Proceed to Section 4.4.4.3-1 to set the microwell strip configuration and the wave-
length options.

4-30
4.4.4.3 Microwell Strip Configuration and Wavelength Options Setup

1. The display (from Section 4.4.4.1, preceding) will show:

F1 READY/MC 1A
___

2. Press DISPLAY/SETUP; the display will show:

F1 SETUP L S P
8 S ABSORB N N Y

NOTE: The cursor is positioned under the well configuration number.

3. Press OPTION to cycle through the 3 microwell configurations: 8, 12, and 16 (2 x 8).

4. When the cursor is positioned under the configuration number desired, press
PROMPT to advance to the next parameter setting; the display will show:

F1 SETUP L S P
8 S ABSORB N N Y

NOTE: The cursor is positioned under the single/dual wavelength designator (“S”
and “D”).

5. Press OPTION to choose between single or dual wavelength (“S” or “D”).

6. When the cursor is positioned under the wavelength option desired, press PROMPT
to advance to the next parameter setting; the display shows:

F1 SETUP L S P
8 S ABSORB N N Y

NOTE: The cursor is positioned under the calculation-type designator.

7. Proceed to Section 4.4.4.4 to set the calculation options.

4-31
4.4.4.4 Calculation Options Setup

1. The display will show:

F1 SETUP L S P
8 S ABSORB N N Y

NOTE: Although ABSORB is selected at this point in the setup, this function is not
really a calculation; it is simply the reading of the optical density of the microwells.

2. Press OPTION; the display will show:

F1 SETUP L S P
8 S RATIO N N Y

3. Press OPTION; the display will show:

F1 SETUP L S P
8 S LINEQU N N Y

4. Press OPTION; the display will show:

F1 SETUP L S P
8 S DIFF N N Y

5. Press OPTION; the display will show:

F1 SETUP LS P
8 S ABSORB N N Y

NOTE: All of the calculation options have been presented at this point and the display
has wrapped around to the first option, Absorbance.

4-32
 6. When the calculation option desired (from Steps 2 through 4, preceding) appears on
the display, press PROMPT to enter that option and advance to the next parameter
setting:

● If the option ABSORB is desired, proceed to Section 4.4.4.5.1 to select the limit
(cut-off) options.

● If the option RATIO is desired, proceed to Section 4.4.4.4.1 to select the ratio
options.

● If the option LINEQU is desired, proceed to Section 4.4.4.4.2 to select the linear
equation options.

● If the option DIFF is desired, proceed to Section 4.4.4.4.3 for the flowchart for
difference calculations.

NOTE: DIFF is similar to ABSORB in that there are no additional options to be

selected once the calculation type is selected.

7. When the cursor is under the calculation option desired, press PROMPT to advance
to the next parameter setting or to the additional calculation option parameter
settings for RATIO and LINEQU.

4.4.4.4.1 Ratio

1. When the calculation option RATIO is selected, the display will show:

F1 SETUP L S P
8 S RATIO N N Y

2. Press ENTER to enter the option RATIO; the display will show:

STDS IN STRIP 1
STD WELL A1

NOTE: The cursor is under the well row designator.

4-33
3. Press OPTION to cycle through the well row options: A1, B1, C1, D1, E1, F1, G1,
and H1.

4. When the letter designator over the cursor is the one desired, press ENTER to enter
your choice and advance to the next parameter; the display will show:

STDS IN STRIP 1
UNITS NONE

5. Press OPTION to cycle through the available measurement units: NONE, UG/ML,
and NG/ML.

6. When the desired units of measurement appear on the display, press ENTER to enter
that choice; the display now will show:

STDS IN STRIP 1
STD CONC = 000.0

NOTE: The cursor is under the first digit of a concentration value.

7. Press OPTION to cycle through the numbers: 0, 1, 2,..., 9 for the first digit.

8. If DISPLAY/SETUP is pressed at any time in this standards setup, the

display will show:

STDS IN STRIP 1
COMPLETE SETUP

This message will stay on the display for approximately 2 seconds and then the screen
will return to the prompting sequence.

9. When the cursor is under the number desired for the first digit, press PROMPT to
enter that number and to advance to the next parameter; the display will show:

STDS IN STRIP 1
STD CONC = 000.0

NOTE: The cursor is positioned under the second digit of the concentration.

4-34
 10. Press OPTION to cycle through the numbers 0 through 10 (as in Step 7, preceding).

11. Use a combination of PROMPT and OPTION to cycle through the numbers for the
remaining digits of the standard concentration.

NOTE: You can enter concentration values from 000.0 to 999.9.

12. When you have the desired concentration displayed on the screen, press ENTER to
enter that number and advance to the next parameter in the set-up display.

13. Proceed to Section 4.4.4.5 to select limits.

4.4.4.4.2 Linear Equation

1. When linear equation is to be chosen, the display will show:

F1 SETUP L S P
8 S LINEQU N N Y

NOTE: The cursor is positioned under the option LINEQU.

2. Press ENTER or DISPLAY/SETUP.

NOTE: Use ENTER if you have new values to enter, use DISPLAY/SETUP if the
program has been set up and needs no change.

1. If DISPLAY/SETUP is pressed, the display will show:

F1 SETUP LS P
8 S ABSORB N N Y

NOTE: The cursor is positioned under the LIMITS HEADER. Proceed to

Section 4.4.4.5.

2. If ENTER is pressed, the display will show:

STDS IN STRIP 1
# OF STDS = 2

NOTE: The cursor is positioned under the standards number.

4-35
3. Press OPTION to cycle among the number of standards available for linear equation
calculations: 2, 3, 4, and 5.

4. When the number above the cursor is the number of standards desired, press ENTER;
the display will show:

STDS IN STRIP 1
1ST STD A1

NOTE: The cursor is positioned under the letter designating the well row.

5. Press OPTION to cycle among the row designators available for linear equation
calculations: A1, B1, C1,...,H1, A2, B2,..., G2.

NOTE: The full 16-well (2 x 8) number of wells are represented by these letter and
number designators. The options that can be cycled through, of course depend on the
well configuration selected earlier in the setup procedures.

6. When the desired strip location designator is displayed on the screen, press ENTER;
the display will show:

STDS IN STRIP 1
UNITS NONE

7. Press OPTION to select the desired concentration units from: NONE, UG/ML, and
NG/ML.

8. When the desired concentration units appear on the display, press ENTER; the
display will show:

STDS IN STRIP 1
1ST CONC = 000.0

NOTE: The cursor is positioned under the first digit of the concentration value.

9. Press OPTION to cycle through the numbers 0 through 9.

10. When the desired first digit of the concentration appears on the display, press
PROMPT; the display will show:

STDS IN STRIP 1
1ST CONC = 000.0

4-36
 11. Use a combination of OPTION and PROMPT to cycle through the numbers and
move the cursor to the adjacent digit.

12. When the desired concentration appears on the display, press ENTER; the display
will show:

STDS IN STRIP 1
2ND CONC = 000.0

13. Use a combination of OPTION and PROMPT to cycle through the numbers
0-9 and to move the cursor under the digit to be changed.

14. When the desired concentration appears on the display, press ENTER; if all standard
concentrations are entered, the display will show:

F1 SETUP L S P
8 S LINEQU N N Y

15. Press PROMPT to advance to the next parameter to be selected.

16. Proceed to Section 4.4.4.5 to select the LIMITS option.

4.4.4.4.3 Difference

1. NOTE: If DIFF is selected, there is no OPTION; that is, pressing OPTION has no
effect.

2. Proceed to Section 4.4.4.5 for the next parameter selection: Setting Limits.

4-37
4.4.4.5 Limit Options Setup

For simplicity, only ABSORB will be followed through the remaining permutations in the
prompting sequence.

1. When PROMPT is pressed after the calculation option is chosen, the display will
show:

F1 SETUP L S P
8 S ABSORB N N Y

NOTE: The cursor is under the LIMITS header.

2. Press OPTION to choose between no limits (“N”) and limits (“Y”).

When no limits are chosen, press PROMPT to advance to the next parameter
selection; the display shows:

F1 SETUP L S P
8 S ABSORB N N Y

The cursor moves to the right and is positioned underneath the serial port header
which also has “Y” and “N” selections available.

3. When limits are chosen, the display shows:

F1 SETUP L S P
8 S ABSORB Y N Y

4. If the limits stored in the reader’s memory do not need to be changed, press
PROMPT to move the cursor to the next parameter.

5. If the limits need to be entered for the first time or changed, press ENTER; the
display will show:

F1 LIMITS SETUP
LIMIT1 = _0.000

NOTE: The cursor is positioned to the left of the limit (cut-off) value.

4-38
6. Press OPTION to toggle between positive values (no sign shown) and negative values
(“-”); for negative values the display will show:

F1 LIMITS SETUP
LIMIT1 = -0.000

7. Press PROMPT to move the cursor to the right under the first digit of the limit value;
the display will show:

F1 LIMITS SETUP
LIMIT1 = -0.000

8. Press OPTION to toggle between “0, 1, and 2” for the number to the left of the fixed
decimal point; for example, the display might show:

F1 LIMITS SETUP
LIMIT1 = -2.000

9. Press PROMPT to move the cursor to the right of the decimal point.

NOTE: For these places, pressing OPTION cycles through the digits “0 through 9”
thereby enabling the operator to select limits from “-2.999” to “2.999.”

10. When the desired value for LIMIT1 is shown on the display, press ENTER to enter
the value and continue the prompting sequence with the display:

F1 LIMITS SETUP
LIMIT2 = _0.000

4-39
11. Repeat the preceding steps to enter the value desired for LIMIT2.

NOTE: Remember that LIMIT1 must be less than or equal to LIMIT2. If LIMIT1 =
LIMIT2 then the resulting printout will show only “+” and “-” calls for the wells; there
will be no “+/-” (indeterminate or gray zone).

If LIMIT1 is greater than LIMIT2, an error message lasting approximately 2 seconds

informs the operator of the problem; the display shows:

F1 LIMITS SETUP
LIMIT1 LT LIMIT2

where “LT” means “less than.” The display then returns to the prompting sequence.

12. If DISPLAY/SETUP is pressed during assignment of the limit (cut-off) values, an

additional error message appears on the display for approximately 2 seconds:

F1 LIMITS SETUP
COMPLETE SETUP

The display then returns to the prompting sequence for setting limits.

13. When the limits setup is complete and the desired values have been entered, press
ENTER to return to the setup display:

F1 SETUP L S P
8 S ABSORB Y N Y

4-40
4.4.4.6 Data Output Options Setup

1. The display shows:

F1 SETUP LS P
8 S ABSORB N N Y

NOTE: The cursor is positioned under the serial output header.

2. Press OPTION to choose between no serial output (“N”) or output (“Y”).

NOTE: If serial port transmission is enabled, the computer must be connected and
turned ON. If the reader tries to transmit data via the serial interface and the com-
puter is not connected, the reader will hang up. The only way to resume operation of
the reader is to turn the unit OFF and then ON again.

3. When the desired option appears on the screen, press PROMPT to advance to the
next parameter selection; the display shows:

F1 SETUP LS P
8 S ABSORB N N Y

NOTE: The cursor is positioned under the parallel output header.

4. Press OPTION to choose between no parallel output (“N”) or parallel output (“Y”).

5. When the desired option appears on the screen, press DISPLAY/SETUP; the display
will show:

F1 READY/MC 1A
___

NOTE: The parameter setup is now complete and you are ready to read an actual
microwell strip.

6. Proceed to Section 4.4.5 to read samples.

4-41
4.4.5 Reading a Microwell Strip

This section is actually a continuation of the preceding section when all of the file parameters
are selected. Perform all of the operations shown in the preceding sections. When you have
completed all of these steps, you should have learned how to use all reader functions and to
operate the Model EL301microwell strip reader.

1. After the calibration procedure is complete, the display shows:

F1 READY/MC 1A
---

if the memory is clear. If the memory is full, the display shows:

F1 READY/MF 1A
---

2. If the memory is full and READ or BLANK is pressed, the display shows the error
message:

CLEAR MEMORY
TO REREAD STRIP

3. Press CLEAR to clear the memory and the display shown in Step 1, preceding will be
shown.

4. If READ is pressed before the reader has been blanked on air or wells, the display
will show the error message:

F1 READY/MC 1A
NO BLANKING YET

4-42
5. If you wish to blank on air, press BLANK before the carrier is placed on the guide
rail; the display will show:

F1 READY/MC 1A
BLANK ON AIR

F1 DUAL/W1 1A
---

NOTE: For this example, dual wavelength is used.

6. If you wish to read a blank well (or several blanks), place the carrier on the guide rail
and slide the carrier along the rail until the first well location is sensed by the reader.
Press BLANK and the display shows:

F1 DUAL/W1 1A
READING A BLANK

F1 DUAL/W1 1A
A1

7. If the carrier is not moved and READ is pressed, the display briefly shows the error
message:

F1 DUAL/W1 1A
WELL IS A BLANK

8. If BLANK is pressed on a well that is already blanked the reader briefly displays the
error message:

F1 DUAL/W1 1A
ALREADY BLANKED

NOTE: If you wish to re-read a blank, press CLEAR.

4-43
9. Slide the carrier to the next well location; the display shows:

F1 DUAL/W1 1A
B1 READ OR BLNK

Decide if you wish to continue blanking or if you wish to read the ABSORBANCES
of any STANDARD wells and then start to read ABSORBANCES of any SAMPLE
wells.

NOTE: Remember that blanks can be carried over to other strips that taken together
comprise a full microplate. Conceivably, you could read all wells in this strip except
one as BLANKS; one well is required to activate the blank-averaging routine of the
reader.

10. Press READ; the display shows:

F1 DUAL/W1 1A
READING A SAMPLE

F1 DUAL/W1 1A
B1

NOTE: Remember that when reading with dual wavelength that no ABSORBANCE
value appears on the display until the well is read at both wavelengths.

11. Slide the carrier to the next well location and again press READ. Continue to read all
of the wells remaining in the strip. (If you are reading a 2 x 8 (16-well)
configuration, slide the carrier off the rail and reposition it on the groove closest to
the carrier edge to read wells A2 through H2.)

12. When you have completed reading the strips at wavelength W1, press the W1/W2
button to begin reading at the second wavelength.

NOTE: Remember to pull the filter paddle out of its receptacle until it clicks into
place for reading at wavelength W2.

13. All BLANKS and STANDARDS that were read at filter wavelength W1 must also be
read at wavelength W2. The reader will not allow you to read SAMPLES until this
has been done.

4-44
 14. When you have completed reading this strip, use the PROMPT and OPTION keys to
change to the next strip number.

15. The reader shows the following display when you change strips:

NEW STANDARDS?
OPTION OR ENTER

16. If you wish to use new STANDARDS for the new strip, press enter. If you wish
to use the same STANDARDS, press OPTION and the display will show:

SAME STANDARDS?
OPTION OR ENTER

Pressing ENTER enables you to use the STANDARDS entered for the first strip.
Pressing OPTION toggles between NEW and SAME STANDARDS.

17. Continue reading all of your strips remembering to position the filter paddle in its
receptacle to correspond to the filter wavelength used.

18. Press DATA OUT at any time you wish to transmit data to the printer and the
external computer. (Be sure that your file setup and equipment configuration are the
same so that this does not cause any problems. Refer to Section 4.3.3.5 for detailed
information.)

4.4.6 Sample Printouts

See Figure 4.4.6-1 for sample printouts from the Model EL301 . The sample printouts in-
clude the results from files set up with different parameters and a sample File Setup
Report.

4-45
 PRINTOUT EXAMPLE

In the printout below, the user selected:

2 x 8-well strip

Blanks: A-1 and A-2

Difference Calculation

---------------------------------------------------------------------------------------------------------------------

File 1 Strip 3C

# TYPE O.D. DIFF

__ ____ ____ ______

A1 b 0.004
B1 b -0.004
C1 - 0.299
D1 - -0.012
E1 - 0.345
F1 - 1.008
G1 - 0.907
H1 - 0.189

A2 - 0.657 -0.653
B2 - 0.620 -0.624
C2 - 0.296 0.003
D2 - 0.287 0.275
E2 - 0.986 -0.641
F2 - 1.209 -0.201
G2 - 0.196 0.771
H2 - +.+++ *.***

Figure 4.4.6-1. Sample Printouts

4-46
 PRINTOUT EXAMPLE

In the printout below, the user selected:

8-well strip
Blank: A-1
Ratio Calculation
C-1 as standard, conc. = 200.0 NG/ML
---------------------------------------------------------------------------------------------------------------------

File 3 Strip 2D

# TYPE O.D. RATIO

__ ____ ____ ______

A1 b 0.000 .0
B1 - 0.604 404.0
C1 s 0.299 200.0
D1 - -0.012 -8.0
E1 - 0.345 130.6
F1 - 1.008 654.2

G1 - 0.907 606.6
H1 - 0.189 126.4
________________________________________________________________________________________________

PRINTOUT EXAMPLE

In the printout below, the user selected:

8-well strip
Blanks: A-1 and B-1
Limits Program
----------------------------------------------------------------------------------------------------------------------
-

File 4 Strip 3C

Limit1 = +0.200
Limit2 = -0.200

# TYPE O.D. LIMITS

__ ____ ____ ______

A1 b 0.004 +/-
B1 b -0.004 +/-
C1 - 0.299 +
D1 - -0.212 -
E1 - 0.345 +
F1 - 1.008 +
G1 - 0.907 +
H1 - 0.189 +/-

4-47
4-48
 5 SAFETY, MAINTENANCE, AND SHIPPING

Service of this instrument should be left to qualified personnel. This section of

the operator’s manual is intended only to describe those procedures necessary
to keep the Model EL301 working properly and accurately on a regular basis.
If a problem develops, contact your Bio-Tek Dealer Representative. If you
need to return the unit for service, be sure to read the shipping instructions in
Section 5.3.

5.1 Electrical Safety

During normal operation, the power line cord is plugged into an AC power
outlet that has a protective earth contact to connect the chassis of the Model
EL301 to ground. Do not disable this protective action by using an extension
cord or cheater plug that does not have a protective conductor.

5.2 Maintenance

5.2.1 Testing the Light Bulb

The light beam emanating from the optics arm on the top of the Model EL301
must be tested to see if it is burned out. The Model EL301 is designed so that
bulb testing is performed before the optics shroud is removed.

1. Turn the Model EL301 ON.

2. Observe the LCD window; it should display the user prompt:

REMOVE CARRIER
THEN HIT ENTER

3. Follow the instructions given on the display as the instrument proceeds through a
calibration phase.

INSERT FILTER W2
THEN HIT ENTER

5-1
 CALIBRATING
WAVELENGTH W2

INSERT FILTER W1
THEN HIT ENTER

CALIBRATING
WAVELENGTH W1

4. When the calibration phase is complete, press the DISPLAY/SET-UP button.

5. See Figure 2.2-1 to locate the hidden buttons in the upper lefthand corner of
the Model EL301 front panel. As noted in Section 2.2.1, there is a hidden
button to the left of the display or centered above the display. Run your
finger along the front panel in this area and you will locate this button.

6. Press the hidden button.

7. Observe the LCD window on the Model EL301 ; it should display:

LAMP GAIN FREQ

OFF 1 _ _ _._

where:

LAMP =Continuous lamp illumination; options: OFF and ON.

GAIN=Gain experienced by the analog circuit; options: 1, 2, 5, and 10.

FREQ=Frequency (3- to 5-digit value).

NOTE: LAMP (ON) GAIN (10) with a 405 nm filter in position should
produce between 6000-to-8000 counts in the frequency display area.

8. Press PROMPT to move the underline cursor under the “L” of LAMP (if it is not
already positioned there). Observe that the LAMP should be OFF as designated by
the word OFF underneath the word LAMP on the LCD.

9. Press OPTION to change the LAMP setting to ON.

NOTE: At this time the light bulb should be ON continuously.

10. If you are having trouble seeing the light beam, darken the working area or place a

5-2
 dark piece of paper over the filter chamber opening in the EL301front panel.

11. If the light bulb is not ON, refer to Section 5.2.1.1 for instruction on how to replace
the light bulb.

5.2.1.1 Lamp/Filter Replacement and Adjustment Procedure

This procedure outlines the replacement of the EL301 lamp assembly for all current versions
of the product.

Replacement - The 301 bulb is housed within the Optics Arm Shroud. To gain access to the
bulb, first remove the Optics Arm Shroud by removing the two socket screws (7/64 wrench
supplied with the instrument) that hold it in place, as shown below in Figure 5.2-1 below.

Figure 5.2-1: Optic Arm Shroud Removal

The lamp will be visible in the rear portion of the Optics arm in one of the two lamp mount
designs, as shown in Figures 5.2-2 and 5.2-3 .

Old Mount: To remove the lamp, unscrew the .050" set screw using the .050 wrench (also

5-3
supplied with the instrument) until the bulb slides back and free of the Lamp Block. Once the
bulb is free of the Lamp Block, remove the connector from the printed circuit board. Reverse
the procedure to install the new lamp. Install the new lamp as far forward in the Lamp Block
as the lamp will go without touching the Optics Arm.

Figure 5.2-2: Old Mount Assembly

New Mount: To remove the lamp, loosen the two 3/32" socket head screws (shown below in
Figure 5.2-3) and slide the lamp out of the mounting springs. Slide the new lamp as far
forward as possible without touching the Optics Arm.

Figure 5.2-3: New Mount Assembly

Performance Check: Once the new lamp is installed, correct operation can be determined in
the following manner:

● Turn on the Model EL301

5-4
 ● Follow the diasplayed instructionsuntil the F1 READY / XXXX prompt is visible.

● Press ther DISPLAY / SETUP key.

● Press the Calibration Check Key. This is a hidden key that appears in different
locations depending on the instrument version (see Figure 5.2-4 below).

Figure 5.2-4: Hidden Key Locations

5-5
 The following display will light when the correct key is pressed.

Lamp Gain Freq

OFF 1 400.00

If the reader resets, you have pressed the wrong hidden key. Attempt the performance check
from the top. The frequency value is an approximate value which may vary depending on
ambient conditions and what type of filter is installed.

Use the PROMPT key to select the Lamp, and the OPTION key to switch the Lamp to ON.
Next, use the PROMPT key to select gain, and the OPTION key to select "10". The 405nm
interference filter must be in the light path at this time.

While there may be some drift in the counts value, the displayed frequency should be between
4000 and 8000 counts. If the count value is not within these limits, some adjustments may be
necessary.

If the count is high or low, the lamp may be adjusted to obtain a higher or lower signal (see
lamp adjustment below) or the gain trim pot can be adjusted within the instrument (see gain
adjustment below). Lamp adjustment should be attempted prior to adjusting the gain.

Lamp Adjustment: Refer to the lamp replacement portion of this document to expose the
lamp for adjustment. The lamp can be moved in three axes to obtain more or less signal (see
Figure 5.2-1). As a rule, sliding the lamp backward in the Lamp Block will de-focus the beam
and lower the count. Rotating the bulb radially may increase or decrease the count value
depending on the individual bulb. Pivoting the lamp in the lamp block will also raise or lower
the count, depending on the individual bulb.

Gain Adjustment: To expose the gain trim pot, remove the left end cap with the 7/64" wrench.
RT1 is for display contrast. RT2 is for gain. Counter-cloockwise rotation lowers gain. Clock-
wise rotation raises the gain.

Filter Check: If a new filter is to be installed, the following check can be done to verify that
the filter does not provide too much light for the individual instrument.

● Access the CALIBRATION CHECK screen (same procedure as accessing the

PERFORMANCE CHECK) SCREEN.)

● Select a gain of 1 and switch the lamp on.

All filters should give no more than 11,999 counts on the didsplay when they are in the
light path. If the value displayed is over 11,999 adjust the gain downward using the 405
nm filter and a gain setting of 1. Recheck all other filters at a gain of 1 and repeat the
process until all the filters are below a count of 12,000. The frequency count for the

5-6
 405nm filter should never be below 4,000. If adjusting the gain or lamp adjustment does
not bring all filters into the correct operational range, contact Bio-Tek's Service Depart-
ment at (800) 242-4685, (802) 655-4044 or FAX (802) 655-3399.

5.2.2 Cleaning Exterior Surfaces

The black exterior of the Model EL301is made of anodized aluminum. The end caps of the
reader body, the microwell strip inserts and the overlay surfaces are made of a polycarbonate
material.

1. Clean the surfaces of the Model EL301 with a mild soap and water solution and
then rinse with clear water. Do not use solvents or abrasive cleaners.

2. The microwell strip carrier and inserts can be sterilized when they have been
contaminated with infectious organisms.

3. Carefully clean the non-skid rubber feet on the bottom of the Model EL301 as
instructed in Step 1, preceding.

NOTE: The Model EL301 is designed to rest on 4 high-friction rubber feet. If

the reader slides around during use, the feet and the working surface are probably
dirty and need to be cleaned.

5.2.3 Cleaning the Optics

The optical components of the Model EL301 should be cleaned periodically to ensure proper
operation. The lens within the optics arm is easily cleaned when the optics arm shroud has
been removed to replace the light bulb (refer to Section 5.2.1).

1. Use lens-cleaning fluid and lens-cleaning tissue to clean the focusing lens mounted
in the optics arm (see Figure 5.2.1-1 for the location of this lens). Refer to Section
5.2.1.1 and 5.2.1.2 to remove the optics arm shroud and expose the focusing lens.

NOTE: Use pressurized air to gently blow away any dust on the lens surfaces
before wiping the lens with tissue. (Pressurized air, designed for cleaning optical
equipment can be purchased at any photographic supply store.) This ensures that
large particles are not ground into the lens surfaces when using the tissue. If this
procedure is not followed the lens could become scratched and yield inaccurate
readings.

2. Clean both sides of the interference filter lens as instructed in Step 1, preceding.

NOTE: Do not try to remove the filters from their protective cups.

5-7
5.3 Storage and Shipping

Storage: The Model EL301 may be stored and shipped in environments with the following
limits:

Temperature -20o -- 60o C

Relative Humidity 0 -- 90% (non-condensing)

NOTE: The instrument should be protected from temperature extremes that can cause
condensation within the instrument, and from corrosive fumes and vapors.

Shipping: When the Model EL301 is shipped to Bio-Tek for service or repair, it must be
shipped in the original packing; other forms of commercially available packing are not recom-
mended and can void the warranty. If the original packing has been damaged or lost, contact
Bio-Tek for replacement packing.

Repack the Model EL301 for shipping as follows:

1. Remove the microwell strip carrier from the raised guide rail.

2. Remove the filter paddle from the paddle receptacle on the Model EL301 .

3. Disconnect the power line cord and the printer and computer cables (if used) from
the Model EL301 .

4. Turn the Model EL301 over and record the full model number and serial number.

5. Obtain a Return Authorization Number from the Bio-Tek Customer Service

Representatives.

6. Reverse the procedures in Steps 1 through 4 in Section 3.1 to finish packing the
instrument.

7. Before sealing the outer shipping box, be sure to include the following information
in the box:

● A statement of what is required of the Bio-Tek Service Department (e.g.,

whether the unit requires calibration, cleaning, or repair).

● A tag that specifies: the full model number, the serial number of the
instrument (found on the bottom panel of the Model EL301 ), and the
Return Authorization Number.

5-8
 ● The name and telephone number of whom Bio-Tek should contact with any
questions.

8. Insure the Model EL301 for its full value.

5.4 Warranty

Refer to the Warranty Card supplied with the Model EL301 for a complete description of
Bio-Tek’s warranty policy.

5-9
5-10
 6 TROUBLESHOOTING

Table 6-1 provides the user with a basic troubleshooting guide for the Model
EL301 Strip Reader. If a problem is not corrected by following the recom-
mendations given in this table, please call the Bio-Tek Service department at:
1-800-242-4685 (1-800-24-BIOTK). If the Model EL301 Strip Reader must
be returned to Bio-Tek for repairs, follow the packing and shipping instruc-
tions in Section 5.3.

Table 6-1. Troubleshooting the Model EL301

PROBLEM CAUSE SOLUTION

Display on front panel not lit. Instrument not plugged in Check power cable connection
properly. (refer to Section 3.2).

Fuse blown Return unit to Bio-Tek for

service

Display shows: Carrier not in position. Place carrier on guide rail

"INVALID WELL (refer to Section 4.4.2).
LOCATION" when taking
reading.
Detent mechanism not in Move carrier until detent
groove on carrier. engages groove.

Magnet (to sense well location) Call Bio-Tek Service

not in carrier. Department.

Setup incorrect or incorrect Check setup for strip

carrier groove placed on guide configuration (1 x 8, 1 x 12, or
rail. 2 x 8.

Display reads: "OD Defective light bulb. Replace light bulb (refer to
OVERRANGE" when taking section 5.2.1.1).
reading.

Incorrect interference filter Check the wavelength number

installed. on the top surface of the filter;
replace if necessary (refer to
Section 3.5).

6-1
 Table 6-1. Troubleshooting the Model EL301 (Continued)
PROBLEM
Display reads: "OD
OVERRANGE" (continued).
Display reads "OD
OVERRANGE" then reads
"+.+++"
No printout on printer when
DATA OUT is pressed.
No serial output to external
printer.
CAUSE
Filter paddle not installed
properly.
Lens, prism, or interference
filter is dirty.
The difference between the
blank vs. the sample absor-
bances is greater than "2.999".
Model EL 301 is not set up to
print.
Loose printer cable connection.
Printer not setup properly.
Model EL301 not set up for
serial output.
6-2
SOLUTION
Slide filter paddle into recep-
tacle on front of Model EL301
until the correct filter is in the
filter chamber and detent
mechanism engages.
Clean with lens paper. (Refer
to Sections 5.2.1 and 5.2.3).
These displays are informa-
tional and do not necessarily
indicate an error that the opera-
tor must remedy.
Check setup and ensure that
printout via parallel port is
selected (refer to Section
4.3.3.5 and 4.4.4.6).
Ensure that all connections are
secure (refer to Section 3.3).
Ensure that the printer is on-
line. Check printer setup (refer
to the printer operator's
manual).
Check setup and ensure that
serial output is selected (refer
to Section 4.3.3.4 and 4.3.3.6).
 Table 6-1. Troubleshooting the Model EL301 (Continued)

PROBLEM CAUSE SOLUTION

No serial output to external Computer not set up. Ensure baud rate is set to 1200
computer (Continued). and that all connections are
secure (refer to computer
operator's manual).

Display reads: "RAM TEST Printer is ON when Model Remember to turn OFF printer,
FAILED" when reader is EL301 is turned ON. turn ON Model EL301, and
turned ON. (A bar on the then turn ON printer. Printer
display may also be observed). must be on-line to receive data.

A bar is displayed when the EPROM or NVRAM problems. Return to Bio-Tek for service.
unit is powered ON.

Display reads: "INVALID Electronics board. Return to Bio-Tek for service.

WELL LOC" when the well in
the carrier is properly posi-
tioned over the reading
chamber.

Lamp does not light at all. Burned out bulb or bulb con- Replace bulb (refer to Section
nector not plugged in securely. 5.2.1.1).

Electronic board problem. Return to Bio-Tek for service.

Unit gives negative O.D. The blanking value in memory Reinitialize RAM and
readings. (RAM) is greater than the O.D. recalibrate for filters being
of the sample. used.

Unit powers up and beeps Sticking key on the keypad. Return to Bio-Tek for service.
constantly.

Unit always reads "0.0" O.D. Light bulb not plugged in. Check connection (refer to
Section 5.2.1.1).

Bulb broken. Replace bulb (refer to Section

5.2.1.1).

Light path blocked. Check path for obstructions.

6-3
 Table 6-1. Troubleshooting the Model EL301 (Continued)

PROBLEM CAUSE SOLUTION

Printer attached to EL301 will Printer not enabled. Change the unit's setup (refer to
not print. Section 3.3).

Printer not powered up or Turn on the printer and ensure

on-line. that it is on-line. Refer to the
printer operator's manual if
additional information is
desired.

Poor printer cable connections. Ensure that all connections are

secure.

EL301 will not transmit data Incorrect baud rate. Change baud rate setting to
via the RS-232C. 1200 baud.

Cable is damaged or not Check cable and connection.

plugged in.

Receiver is configured Ensure that he communication

incorrectly. protocol is correct (refer to
Section 3.4).

EL301 fails to program NVRAM has not been Initialize the NVRAM.
correctly. initialized

EL301 indicates that data Key hit twice by mistake. Practice pressing keys to
aborted when the serial data is become more secure in using
transmitted. the unit.

6-4