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April 2013
Introduction
Hydroquinone is considered the primary topical ingredient for inhibiting melanin production. Its
components have potent antioxidant abilities. Topical hydroquinone comes in 2% (available in
cosmetics) to 4% (or more) concentrations (available from a physician or by prescription), alone
or in combination with tretinoin 0.05% to 0.1%. Research has shown hydroquinone and tretinoin
to prevent sun- or hormone-induced melasma.
Hydroquinone is a strong inhibitor of melanin production, meaning that it prevents dark skin
from making the substance responsible for skin color. Hydroquinone does not bleach the skin but
lightens it, and can only disrupt the synthesis and production of melanin hyperpigmentation. It
has been banned in some countries because of fears of a cancer risk.
Instruments
Automatic Electrophoresis Instrument equipped with UV-visible diod array detector.
Procedure
Sample preparation
Data analysis
Bibliography
Claudia Desiderio, L. O. (2000). Analysis of hydroquinone and some of its ethers by using
capillary. Journal of Chromatography , 489-496.