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ARTICLE IN PRESS

Resonance Tube or Lax Vox?


*Jaana Tyrmi, †Vojtěch Radolf, †Jaromír Horáček, and *Anne-Maria Laukkanen, *Tampere, Finland, and †Prague, Czech
Republic

Summary: Objective. This study compares the flow resistance of Resonance tube (RT) and Lax Vox tube (LVT)
when submerged 2 cm and 10 cm in water, as well as phonation into the tubes in these conditions.
Methods. In the in vitro experiment, the air pressure for flow rates of 60–600 mL/s was measured at the tube inlet,
when the outer end of the tube was submerged 2 cm and 10 cm below water surface in 30°, 45°, and 90° angle. In the
in vivo experiment, 14 subjects phonated in habitual loudness and loudly into both tubes, with the outer end 2 cm and
10 cm in water. RT was immersed in a 45° angle and LVT in a 90° angle in water. Oral pressure, contact quotient from
electroglottographic signal, and sound pressure level were studied. Sensations during phonation were reported in an
interview.
Results. Flow resistance was slightly lower with LVT than with RT, and slightly lower for smaller immersion angles.
In habitual loudness, transglottic pressure and frequency of oral pressure variation were lower for LVT phonation and
amplitude of oral pressure variation was higher for LVT 2 cm in water. Some subjects preferred RT, whereas others
preferred LVT or reported no differences between them.
Conclusions. The tubes differed slightly in flow resistance. Higher oral pressure oscillation with LVT 2 cm in water
may offer stronger massage effect on vocal folds.
Key Words: Flow resistance–EGG–Voice therapy–Water resistance therapy–Air pressure in phonation.

INTRODUCTION of Resonance tube treatment of students with mild dysphonia.


Voice training and therapy utilize phonation into tubes to improve Paes et al7 found increased phonatory comfort, improved per-
voice quality. Phonation into a tube that artificially lengthens and ceptual voice quality, and decreased instability, subharmonics,
narrows the vocal tract increases flow resistance (mean air pres- and noise in dysphonic patients’ voices immediately after water
sure divided by mean airflow). Phonation into tubes is performed resistance exercising with Resonance tube. Mailänder et al4 re-
so that the outer end is either free in the air or immersed in water.1 ported improved perceptual voice quality and self-evaluation of
The latter is called “water resistance therapy.” In Finnish tradi- voice and increased sound level in voice range profile in healthy
tion, glass Resonance tubes (24–28 cm in length, 8–9 mm in inner teachers after 3 weeks of Lax Vox training.
diameter), lanced by Sovijärvi,2 are used. The depth of immer- So far, few studies exist on the effect on phonation of the length
sion in water in a bowl is 2–10 cm (sometimes even 15 cm). and diameter of Resonance tube. Laukkanen compared phona-
Shallow immersion depth, which offers lower flow resistance, tion into three glass tubes (26 cm, 27 cm, and 28 cm in length,
is used for hyperfunctional voice disorder, whereas deep im- and 9 mm in inner diameter) of two female subjects.8 The outer
mersion with high flow resistance is used for hypofunctional voice end of the tubes was free in the air. No systematic differences
disorder, in order to increase compensatory adductory activity.2,3 in the effects of the tubes were found. A recent study9 investi-
For children, Sovijärvi recommended tubes of 24 cm in length gated the effect of phonation into Resonance tubes of 26 cm and
and 8 mm in inner diameter. For adults, the recommended length 28 cm in length on vertical position of the larynx and oral pres-
of tubes depended on voice category: For altos and basses, longer sure in two subjects. The distal end of the tube was in water.
tubes (28 cm) were recommended, whereas for sopranos and Tube length did not seem to have a systematic effect on the results.
tenors shorter tubes (26 cm) were regarded best. In addition to In general, it is known that the length and diameter of a tube
glass Resonance tubes, flexible silicon tubes, called Lax Vox tubes affect the impedance of the tube,10,11 with the diameter having
(length of 35 cm, inner diameter of 9–12 mm), are nowadays used a stronger effect than the length. Furthermore, flow resistance
for water resistance therapy.4,5 The recommended immersion depth (mean pressure divided by mean airflow) is naturally higher when
for a Lax Vox tube is 2–7 cm, and typically a bottle is used instead a tube is in water than when it is free in the air,12,13 and higher
of a bowl. when the immersion is deeper. It is plausible that the immer-
Various beneficial effects have been reported after undergo- sion angle also affects the flow resistance of the tube.
ing water resistance therapy. Simberg et al6 reported improved Among clinicians, there has been an ongoing discussion
perceptual voice quality and fewer vocal symptoms after 7 weeks whether one should use glass Resonance tube or Lax Vox tube
for water resistance therapy. Some speech therapists prefer a glass
Accepted for publication October 27, 2016.
Conflicts of interest: The authors have no conflicts of interest to report.
Resonance tube as it seems to give stronger sensations of res-
From the *Speech and Voice Research laboratory, University of Tampere, Tampere, Finland; onance vibrations on the lips and because it is easier to clean.3
and the †Institute of Thermomechanics, The Academy of Sciences of the Czech Repub-
lic, Prague, Czech Republic.
Other therapists prefer Lax Vox tube because it is more practi-
Address correspondence and reprint requests to Jaana Tyrmi, Speech and Voice Research cal and non-breakable, and offers the possibility of inserting it
Laboratory, School of Education, University of Tampere, Virta, Åkerlundinkatu 5, 33100
Tampere, Finland. E-mail: jaana.tyrmi@uta.fi
in a straight angle into a bottle to avoid splashing of water from
Journal of Voice, Vol. ■■, No. ■■, pp. ■■-■■ a bowl and to help in keeping the head position natural, that is
0892-1997
© 2016 The Voice Foundation. Published by Elsevier Inc. All rights reserved.
not bent down. It has also been mentioned that a Lax Vox tube
http://dx.doi.org/10.1016/j.jvoice.2016.10.024 ensures a better jaw position due to its slightly wider diameter.5
ARTICLE IN PRESS
2 Journal of Voice, Vol. ■■, No. ■■, 2016

To the best of our knowledge, no systematic study exists so far


on the differences between Resonance tube and Lax Vox tube,
neither as it comes to the flow resistance properties of the tubes
as such nor as it comes to the effect the tubes have on phonation.
Hence, this study compares (1) the flow resistance of Resonance
tube and Lax Vox tube in vitro as the tubes are submerged 2 cm
and 10 cm in water, and (2) the characteristics of phonation into
Resonance tube and Lax Vox tube with the outer end immersed
2 cm and 10 cm in water in a bowl. The immersion depths of 2 cm
FIGURE 2. A. The experimental setup for studying phonation into
and 10 cm were chosen as they represent shallow and deep im-
the tubes. Photo published with permission of the person. B. Shutter-
mersion and are most often used in clinical practice.
ing of the distal end of the tube under water at random intervals with
finger.
METHODS
Measurement of flow resistance of the tubes
The flow resistance of a glass Resonance tube (27 cm, 9 mm) Recording of the subjects
and a silicon Lax Vox tube (35 cm, 10 mm) was measured as Fourteen volunteers with no known pathology of the larynx or
follows. The inlet of the tube was attached to a flow source voice (6 males, 8 females, mean age of 43 years) were re-
(Figure 1). The air pressure (backpressure, P(back)) for various corded. Six subjects (4 females, 2 males) had taken at least their
steady flow rates was measured at the tube inlet (1 cm upstream bachelor’s degree in vocology and were familiar with Reso-
of the tube), when the outer end of the tube was submerged 2 cm nance tube and Lax Vox exercising. The other subjects (4 males,
and 10 cm below water surface in an aquarium. Compressed air 4 females) were not familiar with vocal exercising. Trained and
(1 atm) was used as the flow source. The airflow rate, as the man- untrained subjects were compared in order to get a representa-
ually controlled parameter, was measured by a floating flowmeter tive sample of both types of potential users of tube exercises,
(EMKO DF3 09K5, EMKOMETER s.r.o., Ledec nad Sazavou, and to find out whether possible differences in phonation into
Czech Republic). The air pressure on the inner wall of the up- the two tubes would be related to the tubes as such or to com-
stream tube was measured with a digital manometer (GDH 07 pensatory strategies that have been learned. The subjects were
AN, with the measurement accuracy of 1 mm H2O, GHM instructed to phonate into the tube a vowel-like sound between
Messtechnik GmbH Standort Greisinger, Regenstauf, Germany). [u:] and [o:], which are the most natural alternatives with lip
The signal was registered by a PC-controlled measurement system rounding (lips around the tube). They phonated three times in
Brüel & Kjaer PULSE 10 (Brüel & Kjær Sound & Vibration comfortable pitch and loudness and three times loudly into a Res-
Measurement A/S, Nærum, Denmark) using a 32.8 kHz sam- onance tube (27 cm) and Lax Vox tube, with the outer end
pling frequency and a 16 bit A/D convertor. Flow values ranging submerged first 2 cm and then 10 cm in water (Figure 2). Each
from 60 to 600 mL/s were used, as they comprise the most typical sustained phonation lasted approximately 5 seconds. Half of the
range measured from humans.14 Three different immersion angles subjects started with Resonance tube and the other half with Lax
were tested: 30°, 45°, and 90°. These angles were chosen because Vox tube. The Resonance tube was immersed in 45° angle in
90° is recommended for vocal exercising with the Lax Vox tube water and Lax Vox tube in 90° angle, as these angles allow the
and 45° for the Resonance tube, and 30° was considered as the best body posture during the task. Recordings were made for
lowest degree that is practical to use when phonating through a oral pressure (MS-110 transducer electronics unit by Glottal En-
tube in water. A movable laboratory support was used for en- terprises, Syracuse, NY; and transducer PT-75, which enables
suring the exact tube position in the water (Figure 1). registering pressures of up to 75 cm H2O), electroglottographic
signal (dual-channel Electroglottograph (EGG), Glottal Enter-
prises), and acoustic signal (AKG head-mounted microphone
C5441 at 6 cm from the lips; AKG, Vienna, Austria). Comput-
erized Speech Laboratory (CSL 4500, KayPENTAX, Lincoln
Park, NJ) was used in the recording (44.1 kHz, 16 bits). Acous-
tic signals were calibrated with a standard sound source to enable
measurement of sound pressure level (SPL). Sensations during
tube phonation were reported in an interview. The questions posed
were the following: (1) Did you feel any differences between
the tubes? (2) If yes, which tube you preferred and why?

Analyses of the signals


The peak oral pressure P(oral) during manual shuttering of the
distal tube end was measured for an estimate of subglottic pres-
sure P(sub), and the mean P(oral) was measured when the tube
FIGURE 1. The experimental setup for measurement of flow resis- was open (Figure 3). These measurements enabled the calcula-
tance of the tubes. tion of the mean transglottic pressure: P(trans) = P(sub) − P(oral).
ARTICLE IN PRESS
Jaana Tyrmi, et al Resonance Tube or Lax Vox? 3

FIGURE 3. Example of oral pressure measurement during tube phonation and random shuttering of the distal end of the tube.

P(trans) was measured as it is the driving force of vocal fold Population variance was investigated by Kolmogorov-Smirnov
vibration. Analyses were made with Soundswell Signal Work- one-sample t test. The sample types (Resonance tube vs Lax Vox
station (Nyvalla DSP, Stockholm, Sweden). Additionally, the tube) were compared using Student’s t test or Wilcoxon signed
frequency and amplitude of peak-to-peak oscillation in P(oral) rank paired t test, depending on the population variance. A non-
during water bubbling were measured (Figure 4). For that purpose, parametric test (Wilcoxon) was used when the values did not
the P(oral) signal was filtered by fourth-order Butterworth digital show normal distribution. The level of statistical significance was
filter in order to exclude acoustic pressure variations from the set to P < 0.05. The SPSS-21 software (International Business
signal. Limits in filtering (cutoff frequencies) were set so that Machines (IBM) Corporation, Armonk, North Castle, New York,
they could reliably exclude both static part (high-pass filter) and the USA) was used in the analyses.
fundamental frequency of vocal fold vibration (low-pass filter)
in all signals. The low limit in filtering was set to 4 Hz and the RESULTS
high limit was set to 30 Hz. Filtering and measurements were Differences between the tubes for the flow
made with Matlab (MathWorks, Natick, MA). The SPL was mea- resistance measured in vitro
sured from the acoustic signal using the Praat software (Paul Figure 6A shows the measured backpressure as a function of
Boersma and David Weenink, version 5.3.55, Amsterdam, The flow rate for both tubes. The differences between the tubes were
Netherlands). Contact quotient (CQ = contact time/period time), small. In the range of 0–0.3 L/s flow rate, which corresponds
derived from EGG, was calculated as it reflects phonation type.15,16 to ordinary phonation,14 the maximum difference considering all
VoceVista (Roden, The Netherlands) was used for automatic cal- immersion angles was only 0.4 cm H2O, which is within the
culation of CQ. A threshold level of 35% of the EGG signal margin of error in clinical measurement of air pressure vari-
amplitude was used to distinguish between open and closed times ables in phonation, especially during phonation into water.
of the glottis (Figure 5). Figure 6B shows the corresponding airflow resistance curves,
where the differences between the tubes are negligible.
Statistical analyses of the data from subjects The pressure drop in a circular tube conveying incompress-
The measured parameters—CQ, P(sub), P(oral), P(trans), fre- ible viscous steady fluid flow depends on whether the flow is
quency and amplitude of P(oral) oscillation, and SPL—were laminar or turbulent. Laminar flow occurs at lower velocities,
described by calculated mean values and standard deviations. when a fluid flows in parallel layers. Turbulent flow is a less

FIGURE 4. Example of determining bubbling frequency and the peak-to-peak amplitude of the filtered oral pressure signal. Frequency of P(oral)
variation = number of (positive or negative) P(oral) peaks per time. Amplitude of P(oral) variation = mean difference Di between consecutive pos-
itive peak and negative peak = sum(Di)/n, where n is number of pairs of + and − peaks in filtered signal. P(oral), oral pressure.
ARTICLE IN PRESS
4 Journal of Voice, Vol. ■■, No. ■■, 2016

TABLE 1.
Differences for Pressure Values for RT and LVT Within
0–0.3 L/s Flow Rate
P(sub) (cm H2O)
Q (L/s) Glass (2 cm) Lax Vox (2 cm) Diff
0.06 2.96 2.96 0.00
0.10 3.11 3.16 -0.05
0.20 3.42 3.42 0.00
0.30 3.82 3.82 0.00

P(sub) (cm H2O)


Q (L/s) Glass (10 cm) Lax Vox (10 cm) Diff
0.06 10.91 10.91 0.00
0.10 11.11 11.06 0.05
FIGURE 5. Example of measurement of contact quotient (CQ) from 0.20 11.42 11.22 0.20
electroglottographic signal. 0.30 11.83 11.62 0.20
Abbreviations: LVT, Lax Vox tube; P(sub), subglottic pressure; RT, Reso-
nance tube.

orderly flow regime that is characterized by eddies which result


in lateral mixing of the fluid layers. In contrast to laminar flow,
turbulence is associated with high Reynolds numbers. Theoret- Results for the subjects
ically for the Reynolds number—Re = U D/ν < 2300, where U No significant differences were observed in CQ, P(sub), P(oral),
is the flow velocity, D is the inner tube radius, and ν is the ki- and SPL between phonation into Resonance tube and Lax Vox
nematic air viscosity—the flow in the tube should be laminar, tube (Table 2). P(trans), instead, was somewhat lower with Lax
and for Re > 4000 turbulent see for example White.17 For the Vox tube in phonation at habitual loudness. The amplitude of
Resonance tube, we can estimate that the flow inside the tube P(oral) oscillation tended to be higher and the frequency of the
should be laminar for the flow rate of Qlam < 0.244 L/s and tur- oscillation tended to be lower with Lax Vox tube.
bulent for the flow rate of Qturb > 0.424 L/s. For the Lax Vox tube, With regard to the subjects’ answers to questions concern-
these boundaries are shifted up to the flow rates of Qlam < 0.271 L/s ing possible preference of the tubes, half of them (7) preferred
for laminar flow and up to Qturb > 0.471 L/s for turbulent flow. Resonance tube, whereas others preferred Lax Vox tube (5 out
In the region between the limits for laminar (Qlam) and turbu- of 14) or did not report sensing any special difference between
lent (Qturb) flows, there is a transition between laminar and them (2 out of 14). The reasons given for the preferred choice
turbulent flow inside the tube. were, for example, a stronger sensation of resonance vibration
The amount of pressure needed for a certain flow rate was on the lips and sensation of less air consumption with the Res-
somewhat dependent on the immersion angle of the tube in water, onance tube, whereas Lax Vox tube was regarded as more pleasant
see for example Figure 6A for Lax Vox tube 2 cm in water. The between the lips, and according to some subjects sensations of
30° immersion angle required slightly less air pressure com- resonance vibrations were felt also on the cheek tissues and
pared with the larger angles. The maximal differences within the forehead.
whole range of flow rates, Q = 0.06–0.6 L/s, were small, however, Figure 8 shows the distribution of the parameter values in terms
just 0.4 cm H2O for Lax Vox tube immersed 30° in water and of the first and third quartiles, median, and the minimum and
0.6 cm H2O for Resonance tube immersed 90° in water. Such maximum values. In general, somewhat higher CQ values (wider
small differences are comparable with the hydrostatic pressure distribution), lower P(trans), and smaller amplitude and fre-
difference given by the tube radius (see the definition of the water quency of P(oral) oscillation were observed for the trained subjects
depth in Figure 1). (Figure 8). The differences were not categorical though, but there
It must be noted that it is difficult to phonate through a glass was considerable overlapping in the values between the groups.
tube in water with 90° immersion angle and yet keep the body
and neck unbent, as it is recommended in voice therapy.3 The DISCUSSION
most natural choice with the Resonance tube seems to be 45°, Flow resistance was slightly lower with Lax Vox tube than
whereas for a Lax Vox tube 90° is recommended.5,18 In that with Resonance tube, and slightly lower for smaller (<90°)
case, the maximal difference between the two tubes would be immersion angles (Figure 6B). Flow resistance was higher for
0.7 cm H2O for 2 cm immersion depth and 0.9 cm H2O for lower flow values (corresponds to soft phonation). The flow pres-
10 cm immersion depth, with a flow rate of 0.6 L/s considered sure results of the present study are in line with those obtained
(Figure 7). Thus, for the flow range that is more typical in in a recent study by Amarante Andrade et al.19 It is possible to
phonation, 0–0.3 L/s, the maximal difference would be 0.05 cm estimate on the bases of their figure that a Resonance tube (26 cm
H2O for 2 cm immersion and 0.2 cm H2O for 10 cm immer- in their case) required at maximum ca 0.25 cm H2O more pres-
sion (Table 1). sure than Lax Vox tube at a flow rate of ca 0.3 L/s, whereas at
ARTICLE IN PRESS
Jaana Tyrmi, et al Resonance Tube or Lax Vox? 5

FIGURE 6. A. Pressure flow curves for Resonance tube and Lax Vox tube when submerged 2 cm and 10 cm in water with three immersion angles.
B. Flow resistance (mean pressure/mean flow) curves for Resonance tube and Lax Vox tube when submerged 2 cm and 10 cm in water with three
immersion angles.
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6 Journal of Voice, Vol. ■■, No. ■■, 2016

FIGURE 7. Pressure flow curves for Resonance tube (glass) submerged in 45° angle and Lax Vox tube submerged in 90° angle in water.

TABLE 2.
Mean Values and Standard Deviations of CQ, Estimated Subglottic, Oral (Supraglottic), and Transglottic Pressure, Fre-
quency and Amplitude of P(oral) Oscillation, and SPL for Phonation into RT and LVT at Two Immersion Depths (2 cm
and 10 cm) and Two Loudness Levels (Habitual and Loud Phonation)
CQ 35% P (sub) P (oral) P (trans) Freq. P (oral) Ampl. P (oral) SPL
Habitual Loudness (%) (cm H2O) (cm H2O) (cm H2O) (Hz) (cm H2O) (dB)
Glass tube 2 cm
Mean 53.8 10.4 4.3 6.1 17.4 1.7 71.7
SD 10.3 3.7 1.4 2.9 1.7 0.7 5
Lax tube 2 cm
Mean 53.8 9.2 4.6 4.6 15.1 2 70.5
SD 12.8 3.1 1.6 2.4 2.5 0.8 2.8
Diff. for tubes (Student / Wilcoxon) ns ns ns p 0,03 p 0,001 p 0,008 ns
Glass tube 10 cm
Mean 55.3 15.7 10.7 7 15.8 1.7 71.6
SD 10.2 3.4 0.9 2.6 2.7 0.7 4.9
Lax tube 10 cm
Mean 54.9 14.6 10.9 5.1 14.6 1.7 71.6
SD 8.1 3 1 2.8 2.4 0.6 3.4
Diff. for tubes (Student / Wilcoxon) ns ns ns p 0,047 p 0,004 ns ns

CQ 35% P (sub) P (oral) P (trans) Freq. P (oral) Ampl. P (oral) SPL


Loud (%) (cm H2O) (cm H2O) (cm H2O) (Hz) (cm H2O) (dB)
Glass tube 2 cm
Mean 54.8 15.9 7.6 8.2 17.8 2.1 77.4
SD 10.9 5.4 2.7 3.9 1.8 0.9 3.4
Lax tube 2 cm
Mean 55.8 15.1 7.7 7.4 16.6 2.3 77.7
SD 12.9 5.1 2 4.8 1.5 0.8 3.8
Diff. for tubes (Student / Wilcoxon) ns ns ns ns p 0,026 ns ns
Glass tube 10 cm
Mean 58.6 23.2 12.9 11.4 16.5 2.1 75.9
SD 10.5 6.7 1.8 5.6 2.1 0.7 7.8
Lax tube 10 cm
Mean 54.9 22.5 13 10.1 16.4 2.3 78.2
SD 12.3 5.8 1.9 5.3 2 0.6 4.1
Diff. for tubes (Student) ns ns ns ns ns p 0,033 ns
Differences between the tubes are studied with t tests; ns = non-significant (P > 0.05).
Abbreviations: CQ, contact quotient; LVT, Lax Vox tube; P(oral), oral pressure; P(sub), subglottic pressure; RT, Resonance tube; SD, standard deviation; SPL,
sound pressure level.
ARTICLE IN PRESS
Jaana Tyrmi, et al Resonance Tube or Lax Vox? 7

FIGURE 8. Distribution of (A) CQ, (B) P(trans), and (C) amplitude and (D) frequency of peak-to-peak P(oral) for trained (N = 6) and un-
trained subjects (N = 8) in different tasks (both loudness together). CQ, contact quotient; P(trans), transglottic pressure; P(oral), oral pressure.

very low flow rates (<0.05 L/s) the opposite was measured. We between phonation into the tubes. P(oral) oscillation during water
did not consider flow rates lower than 60 mL/s, as measure- resistance exercising results from water bubbling. Bubbling starts
ment accuracy gets low then, and because very low flow rates as the air pressure inside the tube slightly exceeds the hydro-
are not typically reported in humans. It seems that when im- static pressure in water, and the bubble separates from the tube
mersion depth in water is the same, the differences in length, end when the buoyancy force acting on the bubble is about the
material, and inner diameter of these tubes have rather little effect same or higher than the aerodynamic force in the tube.20 The
on the flow resistance. Some differences were observed in pho- frequency of bubbling is known to increase with flow rate and
nation of the subjects though. with a decrease of tube orifice, and to be practically indepen-
P(trans) was somewhat lower with Lax Vox tube in phona- dent on the depth of orifice immersion if it is between 2.54 cm
tion at habitual loudness, which could imply softer phonation.12 and 25.4 cm.21 Thus, for the same airflow rate, a slightly lower
CQ is typically smaller with softer phonation, but in our results bubbling frequency would be expected for Lax Vox tube com-
there were no significant differences in either CQ, P(sub), or SPL pared with Resonance tube. On average, the frequency of P(oral)
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8 Journal of Voice, Vol. ■■, No. ■■, 2016

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