Code No: RR312303 Set No.

1
III B.Tech I Semester Supplimentary Examinations, February 2008
BIO-CHEMICAL ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
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1. (a) Define the following terms: order of a reaction, molecularity, elementary and
non-elementary reactions.
(b) Explain the term steady-state approximation ? [8+8]

2. Define Batch Growth? Explain about the growth patterns and Kinetics in Batch
culture? [16]

3. The Homogenous Gas Reaction A → 2 B is run at 1000 C at a constant pressure

of 1 atm in an experimental Batch Reactor. The Data in Table given below were
obtained starting with Pure A. What size of Plug flow reactor operated at 1000 C
and 10 atmosphere would yield 90% conversion of A for a total feed rate of 10 Msecol ,
the feed containing 40% inerts? [16]

Time,Min 0 1 2 3 4 5 6 7 8 9 10 11
V
Vo
1.0 1.2 1.35 1.48 1.58 1.66 1.72 1.78 1.82 1.86 1.88 1.91

4. Equimolar quantities of A, B and D are continuously fed to a mixed flow reactor.

The elementary reactions that proceed in the reactor are as follows:
K1 K2
A + D −→ P; B + D −→ Q

Given K1 /K2 = 0.2, calculate the fraction of ‘P’ forced when

(a) 50% of A is consumed and

(b) 50% of D is consumed [8+8]

5. Explain Rate, Temperature and conversion profiles for exothermic and endothermic
Reactions in Adiabatic flow Reactors? [16]

6. Develop an expression for external age distribution of N number of equal sized back
mixed reactor in series assuming tank in series model holds good. [16]

7. Determine the residence time distribution for an isothermal tubular-flow reactor in

which the liquid is in laminar flow in an annulus of inner radius r1 and outer radius
r2 . Take α=r
 1 /r 2 . the velocity inthe Axial direction at any radius r is given by
 2
1−α2
u(r) = A 1 − rr2 + 1n 1n r
( α1 ) r2
Express residence time distribution in terms of mean residence time θ . [16]

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Code No: RR312303 Set No. 1
8. The Reactor with a rate expression µ= µmaxS
Ks+S
is carried out in a series of two stirred
tanks of equal size. Calculate the dilution rate required to reduced the substrate
concentration to 50 gm/lit with sterile feed given µ.max = 1hr−1 S0 =20 gm/lit
Ks =2 gm/lit Yx/s =0.6? [16]

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Code No: RR312303 Set No. 2
III B.Tech I Semester Supplimentary Examinations, February 2008
BIO-CHEMICAL ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. A liquid phase reaction of the type A → R is to be carried out in a batch reactor.

The rate of reaction at different concentrations are given below.
M oles
CA lit
0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 1.0 1.3 2.0
M oles
-rA litmin
0.1 0.3 0.5 0.6 0.5 0.25 0.1 0.06 0.05 0.045 0.042
How long each batch is to be reached for the concentration to drop from CAO
= 1.3 mol/litre to CAf = 0.3 mol/lit ? [16]

2. Enumerate in detail various environmental conditions that effect the Growth kinet-
ics? [16]

3. The Data in table given below have been obtained on the decomposition of Gaseous
reactant ‘A’ in a constant Batch Reactor at 1000 C. The stiochiometry of the re-
action is 2A → R+S. What size plug flow reactor (in liters) operating at 1000 C
and 1 atm can treat 100 Mol/hr in a feed consisting of 20% in its to obtain 95%
conversion of A? [16]

Ts ec 0 20 40 60 80 100 140 200 260 330 420

PAatm 1.0 0.8 0.68 0.56 0.45 0.37 0.25 0.14 0.08 0.04 0.02

4. A and B react with each other as follows

2A → R rR = K1 CA 2
A+B → S rS = K2 CA CS
2B → T rT = K3 CB 2
Find what rato of A to B should be maintained in a mixed reactor so as to maximize
the fractional yield of desired products ? [16]
ol
5. For the optimum temperature Progression in a plug flow Reactor, CAO = 4M liter
,
M olA 0 0
FAO =1000 M in , XA = 0.8, TM in =5 C, TM ax =95 C and feed and product both at
250 C, how much heating and cooling would be needed. [16]

(a) For the feed stream?

(b) In the Reactor itself?
(c) For the stream leaving the Reactor?

6. A liquid phase reaction is currently carried out commercially in a series of three,

equal volume stirred tank reactors operating isothermally. It is planned to replace
these reactors with a single tubular flow reactor. To obtain the same conversion as

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Code No: RR312303 Set No. 2
in stirred tank equipment for the same Vt /Q; what degree of dispersion of Necessary
in the tubular reactor. Determine the value of DL /UL , if the flow in the tubular
reactor could be represented by dispersion model ? [16]

7. Explain the role of RTD, State of Aggresation, earliness of mixing in determining

reactor behaviour? [16]

8. Explain in detail the stiochiometry involved in the cell growth? [16]

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Code No: RR312303 Set No. 3
III B.Tech I Semester Supplimentary Examinations, February 2008
BIO-CHEMICAL ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. (a) Define the following terms: order of a reaction, molecularity, elementary and
non-elementary reactions.
(b) Explain the term steady-state approximation ? [8+8]

2. (a) Explain the significance of dissolved oxygen stat?

(b) Explain the significance of PH stat? [8+8]

3. Explain in detail about the method employed in estimation of microbial biomass?

[16]

4. Under appropriate conditions A decomposers as follows

K K
A−→R− →S K = 0.1 M in−1
R is to be produced from 1000 liter/hr of feed in which CAO =1 mol/lit. CRO =
CSO = 0.

(a) What size plug flow reactor will maximize the yield of ‘R’ and what is the
concentration of R in the effluent stream from the reactor.
(b) What size mixed reactor will maximize the yield of ‘R’ and what is CRM ax in
the effluent stream from this reactor. [8+8]

5. Explain Rate, Temperature and conversion profiles for exothermic and endothermic
Reactions in Adiabatic flow Reactors? [16]

6. (a) Explain the significance of Residence Time distribution data and how the same
may be used in the design of non-ideal flow reactors ?
(b) Explain the significance of E, F and C curves. How these functions are inter-
related ? Draw the E and F curves for ideal plug flow reactor and mixed flow
reactor respectively ? [8+8]

7. Calculate the conversion in a laminar flow tubular reactor for a second-order reac-
−
tion A+B → C for which K2 = 100 cm3 /gmols and mean residence time θ =10s.
The feed concentration of both reactors is the same 10−3 gmol/cm3 . Neglect the
molecular diffusion so that flow is segregated? [16]

8. Explain in detail the stiochiometry involved in the cell growth? [16]

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Code No: RR312303 Set No. 4
III B.Tech I Semester Supplimentary Examinations, February 2008
BIO-CHEMICAL ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Derive the michaelis - menten kinetic model for a single substrate, irreversible
enzyme catalysed reaction with the following mechanism. [16]
E + S ⇔ ES → P + E

2. (a) What are the advantages of continuous Bioreactors over Batch Bioreactors?
(b) If continuous Bioreactors have so many advantages over batch bioreactors,
why they are not widely used in industry? [8+8]

containing A (CAO =0.01 M ol

1lit


3. One Gaseous feed stream M in Lit
and a second gaseous
feed stream (3 lit/min) containing B/CBO =0.02 Mol/lit) enter a Mixed Reactor/lit
in volume and react to form a whole host of products R, S,T analysis of the out
going stream of 6 lit. Min shows that CAF =0.0005 Mol/lit and CRF =0.001 Mol/lit.
All flow rates and concentration are measured at uniform temperature and pressure
of the reactor. Find the rate of reaction of ‘A’ and the rates of temperature of ‘R’
in the reactions? [16]

4. Equimolar quantities of A, B and D are continuously fed to a mixed flow reactor.

The elementary reactions that proceed in the reactor are as follows:
K1 K2
A + D −→ P; B + D −→ Q

Given K1 /K2 = 0.2, calculate the fraction of ‘P’ forced when

(a) 50% of A is consumed and

(b) 50% of D is consumed [8+8]

5. The first order Reactions as shown in figure below 5

Figure 5
K1 = 109 e− 6000
T

K2 = 10T e− 4000
T

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Code No: RR312303 Set No. 4

K3 = 108 e− 9000
T

K4 = 1012 e− 12000
T

are to be run in two mixed flow reactors in series any where between 10 and
900 C. If the Reactors may be kept at different temperatures, what should these
temperatures be for maximum yield of ‘S’ ? Find this fractional yield? [16]

6. Water is drawn from a lake flows through a pump and passes down a long pipe in
turbulent flow. A slug of tracer enters the intake line at the lake and is recorded at
two locations in the pipe L meters apart. The mean residence time of fluid between
recording points is 100 sec and the spread in the two recorded signals is
σ12 = 800 sec2 ; σ22 900 sec2
What would be the spread of C curve for a section of this pipe, free from end effects
and of length L/5 ? [16]

7. Discuss the Techniques employed in Diagnosing the ills of operating equipment with
regard to Non ideal flow ? [16]

8. Explain in detail the stiochiometry involved in the cell growth? [16]

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