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The Drosophila wt has red eyes. Consider an offspring bearing a null allele named
white. Would color eyes would you expect in white +/ -? In white +? In white white -/ -
?
-Longer sequences that carry the genes they require for mobility plus other genes as well,
e.g. transposons
→composite transposons = several genes contained between two IS elements orientated
as inverted repeats; (the IS itself allows the mobility); frequently the carried genes are r-
determinants
→simple transposons = have shorter inverted repeats plus their own transposase gene
Long transposable sequences – bc have more genetic information and able to replicate
itself.
Replicative transposition – element replicated and moved to new site, old version stays
behind (copy & paste)
Conservative transposition – element reinserts to new spot but with no replicate left
behind (cut & paste)
-excision =
→replicative transposition (Class I):
-RNA intermediary reverse transcribed to DNA
-element replicated and moved to new site, old version stays behind, e.g. COPY and
paste
-responsible for genome expansion
If the HIS4 mutants derive from retroviruses, why are they ‘trapped’ inside the
yeast genome? Retroviruses generally have the ability to move freely from organism
to organism.
How is it possible someone could be infected with HIV and it was ‘undectable’ and
could only be detected by antibodies?
Hiv is provirus, inserts its genes in host cell, isn’t circulating inside body or blood
stream. Person producing antibodies for it and that how you can detect it
-SINES (short interspersed nucleotide elements), (100-300 bp) ~13% of human genome
→do not encode their own reverse transcriptase;
LINEs may activate them, however
→Alu most abundant human SINE, 1 million++ copies
→a restriction site from Arthrobacter luteus
→most SINEs inactive; some transpose, cause disease
→Alu insertion disrupts VIII & IX (coagulation factors)
Explain the rationale behind the naming of the Sleeping Beauty Class 2 transposon.
Transposons, methylated inactivated
Why is there a difference between the diploid C-value and the haploid C-value in
human males and females?
Diploid: male X Y (Y IS SMALL)
Female X X (X is larger, like double the males)
What would be the safest haven for a SINE, centric heterochromatin or telomeric
chromatin? Exonic insertion or intronic insertion? A promoter region or a enhancer
region?
If you were a rice breeder, would you be more concerned about MITES, SINES, or
LINES?
Mites bc in plants only?
Why are some genomic regions considered ‘selfish’? Plasmids, toxin, antitoxin, or
linked genes Would you expect a housekeeping gene to be selfish? Depends
Why is premature termination the most common problem with active transposable
elements?