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Genetically-defined human cell lines

for use in biomarker discovery, assay validation, and as clinical reference standards

Introducing X-MAN - A model cancer patient

Using GENESIS™, a proprietary human-genome editing platform, HDx is able to recapitulate
patient genetics within a contextually-relevant human cell line. X-MAN human isogenic
disease models have been likened to “patients-in-a-test-tube” and are being used by
academic, biotechnology and pharmaceutical customers to identify novel biomarkers and
disease signatures at the transcriptomic, proteomic and metabalomic level that can be used
to identify which patients might respond best to putative therapeutic agents.
Furthermore, by introducing specific genetic alterations into contextually-relevant cell lines,
HDx is able to supply gold-standard genomic reference materials to CLIA labs for validation,
standardization and quality control of existing companion diagnostic regimens.
GENESIS is a virally-mediated homologous recombination technology that is orders of
magnitude more efficient in performing gene targeting in somatic human cell types over
previous plasmid based technology. Genesis is used to create X-MAN™ (gene-X, Mutant
And Normal) human cell lines by knock-in, or knock-out of specific mutations (including mutant and normal
SNP’s) at the endogenous level. isogenic human cell lines

Available genomic controls and standards Available genomic reference materials

The following matched (mutant and normal) genotypes are immediately available Human isogenic matched genomic DNA
off-the-shelf as gDNA extracts or under custom order as Formalin-Fixed, Paraffin-
Embedded cell lines. All mutations available in any cell line
Frequency of mutation recapitulated
50 ng or 500ng pack sizes Recommended ratio: 99:1 to 50:50
Supplied by courier on blue ice Fully characterized and QC'd
Product Insert and COA supplied Why X-MAN genomic DNA?
GENESIS is unique in its ability to engender both knock-out and knock-in mutations at any
endogenous locus, in any human cell line. This enables the precise modelling of genetic
abnormalities at the same frequency as occurs in a real patient’s tumours, meaning that X-MAN
Catalogue no. Background Genotype
gDNA is this probably the most meaningful control material available.
HD101-012 MCF10A B-Raf (V600E) floxed / +
High-quality controls are becoming increasingly important due to the variable methods and
HD101-002 MCF10A PI3Kα (E545K) processes exhibited by current detection platforms and the highly disparate kinds of controls being
HD101-011 MCF10A PI3Kα (H1047R) applied with them. X-MAN gDNA is a sustainable source of defined genomic control material
which can help customers to standardize genomic testing regimens across different platforms
HD101-010 MCF10A EGFR (del E746-A750)
ensuring the best outcomes for patients.
HD103-002 SW48 K-Ras (G13D)
HD103-006 SW48 K-Ras (G12C) Control standard Flaws
HD103-007 SW48 K-Ras (G12V) Oligos Artificial, no human relevance, variable PCR efficiencies from gDNA
HD103-009 SW48 K-Ras (G12A) Synthesis homogeneity?
HD103-010 SW48 K-Ras (G12R) Plasmid/BACs etc. Improved homogeneity but still artificial
HD103-011 SW48 K-Ras (G12D) Patient samples Non-renewable, highly heterogeneous, ethical barriers
HD103-013 SW48 K-Ras (G12S) Established cell lines Not available for majority of tumour mutations, no matched normal
Order now Cell line mixtures Non-matched, variable PCR efficiencies, controls not normal
Order now by calling +44 (0) 1223 655583 or e-mail info@horizondx.com

Horizon Diagnostics Ltd. Building 7300, IQ Cambridge, Waterbeach, Cambridge CB25 9TL United Kingdom Tel: +44 (0)1223 655580 Fax: +44 (0)1223 862240 E mail: info@horizondx.com Web: www.horizondx.com

A member of the Horizon Discovery Group