MATERIALS AND SUPPLIES

APPENDIX TABLE 1: A: LABORATORY APPARATUS AND EQUIPMENTS (FOR MEDIA PREPARATION)

MATERIALS AND SUPPLIES CAPACITY/ SPECS. Quantity Price Amount

1. pH meter Handy 1
2. Top loading digital weighing 1000g x 0.0001 1
scale capacity
3. Hot plate magnetic stirrer 1
4. Magnetic stirring bar 12” x 5/16 2
5. Magnetic retriever 12” long 1
6. Graduated cylinder 10 ml glass 3
7. Graduated cylinder 100 ml glass 12
8. Graduated cylinder 1000 ml glass 3
9. Beaker 1000 ml glass 12
10. Beaker 2000 ml glass 12
11. Beaker 5000 ml glass 3
12. Amber bottles 2.0 liter 12

13. Refrigerator 7 feet 1

14. Sterilizer 1 pc 1
15. Pressure cooker (U.S. Made) 40 liters 1
16. Fire extinguisher 6.5 kgs 1
17. Air conditioning units 2.0 HP 1
18. Casserole 1 set Stainless/food 1
grade

19. Gas stove two burners La Heavy duty 1

Germania
20. LPG tank with content 50 kgs 1
21. LPG tank refill 50 kgs 1
22. Stirring rods 12” 2 pieces 2
23. Automizer / hand sprayer 3 pieces 3
24. Plastic trays 25 pieces 25
25. Laboratory Gowns Color white/ long 6
sleeves
26. Head cap Disposables 6
27. Slippers Plastic 5
28. household gloves Disposable 6
29. Apron Cloth 3
30. Disposable lighter 1 box
31. Detergent powder 50 kilograms/sack 6
32. Aluminum foil 8 “5m 10 rolls
33. Alcohol 70% Galloon 6
34. Dispensing table 1
35. Pail with cover
Small 4 4
 Medium 3
Large 2
36. Pipette 1ml 3 3
0.5 ml 3 2
37. Culture bottles small w/cover 5,000 5,000
38. Culture bottles big w/ cover 10,000 10,000
39. Volumetric flask 1000ml 2 2
40. Volumetric flask 2000ml 1 1
41. Weighing boat 1
42. Spatula for reagents 1 set
TOTAL

APPENDIX TABLE 1: B MATERIALS FOR INOCULATION ROOM

Materials Quantity Price Amount

1. Wooden chamber set 3
2. Aircon (Split type) 1
3. Computer chair for chambers 3
4. Surgical blades Japan 1 box
5. Forceps: scissors forcep 3
6. Thumb forcep 10” 3
7. Scalpel 4” 3
8. Lamps 3
9. Laboratory gown 6
10. Automizer/ sprayer 3
11. Denatured alcohol 6 galloons
12. Head cap and mask 6 sets
13. Slippers 4 sets
14. Rubbing alcohol 12 bots
15. Scalpel and forcep holders 3 pieces
16. Carts (2 layers) 3
17. Lighter 1 box
18. Chamber arm cover 6 pairs
TOTAL

APPENDIX TABLE 1: C MATERIALS FOR CULTURE ROOM

Materials Specifications Price Amount

1. Shelves 6x2x6’ (10pcs) 5,500.00 55,000.00

2. Fluorescent lamps 20 watts (100 pcs) 250.00 25,000.00
3. Aircon 2.0 HP 25,000.00 25,000.00
4. Ladder 6’ 3,000.00 3,000.00
 TOTAL 108,000.00

APPENDIX TABLE 1: D MATERIALS FOR SUCKER PREPARATION

Materials Specs Price Amount

1. Chopping board Wooden and plastic heavy duty
2. Corm /Sucker 1000 pcs 10.00 10,000.00
3. Knife big and small Stainless
4. Zonrox bleach 6 gals
5. Powder detergent 50 kilograms/sack
6. Pails 5 liter
10 liter
15 liter
7. Aluminum foil 5 rolls
8. Slashing bolo 1
9. Tumbling bolo 1
10. Cotton rolls
TOTAL

APPENDIX TABLE 1: E MATERIALS FOR MEDIA PREPARATION

Materials Quantity Price Amount

1. Dispensing Table 1 3,500.00 3,500.00
2. Plastic Chairs 6 600.00 3,600.00
3. Funnels (for 3 25.00 150.00
dispensing)
4. Trays for (media bots) 4 250.00 1,000.00

TOTAL 8,250.00

APPENDIX TABLE 1: E MISCELLANEOUS SUPPLIES

Materials Quantity Price Amount

1. Ball pen BBGR 4 boxes
2. Pentel Pen BB 2 boxes
3. Record books 2
4. Scotch Tape 2 rolls
5. Packaging Tape 2 rolls
6. Broom 3 pieces
7. Mop 3 pieces
8. Shoe racks 2 pieces
9. Tissue paper 12 rolls
10. Plastic bags 1 roll
 11. Gauge Bandage 1 roll
12. Office supplies

TOTAL

APPENDIX TABLE 2: CHEMICALS TO BE USED IN THE TISSUE CULTURE LABORATORY

Name of Chemicals Grade Packing

Ammonium nitrate AR 1 kilo
Potassium Iodide AR 500g
Cobalt Chloride AR 100g
Boric Acid AR 500g
Manganese Sulfate AR 500g
monohydrate
Zinc Sulfate AR 500g
Sodium Molybdate AR 100g
Nicotinic acid AR 100g
Pyridoxine HCl AR 25g
Activated Charcoal Powder AR 500g
Sodium Hydroxide AR 1 kilo
Buffer Solution pH4 AR 1 liter
Formalin (Formaldehyde) AR 2.5 liter
Potassium permanganate AR 500g
Agar AR 5 kilos
Ethyl alcohol AR 2.5 liter
Edta (Sequestrine agar) AR 250 g
IAA AR 5g
Glycerine AR 2.5
Copper Sulphate AR 500 g
 Schedule of Activities Year 1 Year 2 Year 3
Q1 Q2 Q3 Q4
1. Reconnaissance and Site
inspection
2. Site preparation and
delineation
3. Laboratory
supplies/equipment
acquisition
4. Laboratory Set-up/
construction of shelves
5. Preparation of Culture media
6. Collection of Explants
7. Inoculation of explants
8. Incubation of plantlets
9. Potting out of explants
10. Recovery and hardening stage
11. Disposal for Marketing

BUDGETARY REQUIREMENTS: ‘000

Items Year 1
Q1 Q2 Q3 Q4 Total Year 2 Total
1. Salary

a) Honorarium 20,000 60,000 60,000 60,000 180,000 180,000

per month
b) Propagator 3x 91,494 91,494 91,494 274,482 365,976 640,458
391x26x3
c) Nursery Aide 19,500 19,500 78,000 175,500
250x26x3
d) Lab aid 3x350x26x3 81,900 81,900 81,900 245,700 245,700 737,100
SUB-TOTAL
2. Laboratory
Apparatus/Equipt.

3. Supplies and
materials

4.Chemicals/Reagents

Grand Total