Present

Trustworthy
LC-100 has already obtained
the following certi cations
LC-100 HPLC system

Head Quarter:
Shanghai Wufeng Scientific Instruments Co., Ltd.

Address: 3F, Building 3, Lane 1343 Tongpu Road, Putuo District, Shanghai. Website: www.wufengtech.com
Tel: 8621-52695700 8621-52695702 8621-52695703 E-mail: service@wufengtech.com
Fax: 8621-52695698 Wufeng forum: www.instrument.com.cn/bbs/forum 500.htm
Postcode: 200333

Branch Company:
Trade Company: Application Support: Machining Center:
Exformma Techlogies (Asia) Co., Ltd. Anhui Wance Food Inspection Technology Co., Ltd. Wufeng Precision Mechanism Co., Ltd.
Address: 11/F., AXA Centre, 151 Gloucester Address: No. 1319 Yan Nan Road, Bengbu, Address: No.10 Xiaowei Business Park, Anfu
Road,Wanchai, Hong Kong. Anhui Province Industrial Park Jian district Jiangxi province
Tel: 852-58820238 Tel/Fax: 86552-2811788 Tel: 86769-8299035

Office:

Beijing Office Chengdu Office Wuhan Office

Address: Rm. 2011, Building A, Boutique Service Address: Rm. 1307, Building 4, Lijingtiancheng, Address: Rm.1801,1 8/F, Modern Building (North
Apartment, No.2 Fangzhuang South Road, No.5 Xiaotian East Street, Wuhou District, Block), No.317 Zhongshan Road, Wuchang
Fengtai District, Beijing Chengdu, Sichuan District, Wuhan
Tel/Fax: 86-13520267114 Tel/Fax: 8628-84400584 Tel/Fax: 8627-88712112
Revision Date：2016/09/28

Guangzhou Office Zhengzhou Office

Address: Rm. 802, 8/F, Golden Sky Tower, No. Address: Rm. 7-2-2415, Manhattan Plaza, No.
83 Middle of Huadi Avenue, Liwan District, 305 Jinshui Road, Jinshui District, Zhengzhou
Guangzhou, Guangdong Tel/Fax: 86371-69350587
Tel/Fax: 8620-29890968
8620-22012998

Tried and Trusted


Leader in China’s HPLC
Dedicated to research and development of HPLC products for the past twenty years,
always providing consistent optimal quality.
Wufeng Instruments has the top market share in the Chinese HPLC market, and has the
leading Chinese HPLC technology.
First Chinese digital control HPLC system.
First Chinese Speedy HPLC.
First Chinese intelligent professional auto-sampler.
Currently more than 6000 users are using the Wufeng & Exformma HPLC system have
been deployed by many famous Chinese and international enterprises and research-ori-
ented universities. Some of our customers include:
Fudan University Xiuzheng Pharmaceutical
Nankai University Watrex Praha, S.R.O
North China Pharmaceutical
Wuhan Academy of Agricultural Sciences
Guangdong Wangda Group
Yangtze River Fisheries Research Institute
China Institute of Measurement and Testing technology
Institute of Coal Chemistry, Chinese Academy of Sciences
Shanghai Institute of Measurement and Testing technology Germany Bayer Pharmaceutical
Zhongshan University Lingrui Pharmaceutical
Szent Isvtan University
MERRO PHARMACEUTICAL
Carrefour Group
East China University of Science and Technology
Huangshi Environmental monitor station
Shanghai Food Research Institute
Group Sante Laboratories MASENZ GROUP SL
Ranhill Powertron Power Plant, Sabah, Malaysia
History and Honors of Wufeng Instruments
Launched HPLC research and develop-
1999 ment business
LC-100 was appraised by experts from
Shanghai Science & Technology Com-
mittee and released to the market. 2004
station software-controlled HPLC.
LC-100 won the “BCEIA” Gold Award
2005 issued by China Association for Instru-
mental Analysis
LC-100 was awarded “Annual New
Product of 2004-2005” by instru-
ments.com.cn and Analytical Instru- 2006
ment Branch of China Instrument
LC-100 HPLC was recognized by the
Society
Science & Technology Committee of
Qingpu District, Shanghai and also
2008 won the “Science and Technology
Progress Award of 2008”
EX1600 HPLC released after successful
According to the overall survey by R&D
instruments.com.cn,Wufeng Instru-
ments was ranked No.1 among Chi- 2009
nese HPLC brands
S-HPLC (speedy high performance
liquid chromatograph) passed Shang-
2010 hai Science & Technology Committee
examination was endorsed by a group
of experts.
“ARCUS 5 auto-sampler” was success-
fully developed and put into produc-
tion.
2011
Wufeng Instruments obtained honor
2012
First Chinese S-HPLC speedy high per-
formance liquid chromatographic
system EX1700 hit the market
2013
LC-100 was elected as an “Excellent
Chinese Instrument” by instru-
2014 ments.com.cn, the only HPLC system
S-HPLC (speedy high performance
liquid chromatograph) EX1700 won
the “BCEIA” Gold Award issued by
China Association for Instrumental
2015
Analysis
Reliable stable infusion and detection system

LC-100
Convenient user-friendly design highlights the human element
Tangible quality that surpasses its Chinese counterparts HPLC Sysrem

Chinese leader in processing craft, every detail is exquisite with quali-

ty from the inside out

customers’ aesthetics. The panel was designed with a focus on humanization.

The instrument enclosure is made of corrosion-resistant material to prevent corrosion

caused by solvent leakage during long-term use.

The new LC-100 is equipped with a semi-preparation system, preparation system, and
binary & quaternary gradient system, bas
tions. It was developed from a single UV detector and augmented with a UV-Vis detec-

(evaporative light-scattering detector), etc.

Displays unit operations in real-time and alerts when consumables

need replacing

detectors), and can display operational status of each part in real-time. Consumable
replacement is fully humanized.

without adjusting the light path. When replacing a piston seal, the piston rod can be
retracted automatically to avoid damage.
Chromatographic
Workstation Software
Two kinds of chromatographic workstation software are provided to fulfill different needs.
You can choose the most suitable system according to the demands of your application.
Workstation Software - Professional Version
This workstation is custom-made for Wufeng Instruments by a world famous
company specializing in professional chromatography software. This company
has provided professional chromatographic workstation software to numerous
internationally renowned chromatograph manufacturers. A variety of high
precision 24-bit chromatography signal acquisition modes enables digi tal
control of the instruments and auto-sampler.
The workstation conforms to data GMP certification, FDA certification, Installation
Qualification/Operational Qualification (IQ/OQ), System Suitability Test (SST) and
other specifications intended to ensure data validity and security. Efficient batch
processing streamlines the whole process of instrument control, auto-sampler
sequence acquisition, automatic integration calibration, and reporting output. It
also features post-processing, chromatogram comparison, re-calibration, data
input and output, and three-dimensional chromatogram processing.
The feature-rich interface is designed to meet diverse analytical demands. The
workstation displays complex functions with graphs and arranges icons logically
in the admin window to match the actual instrument sequence.
The software can control up to 400 instruments that are compatible with each other,
and can collect one to four independent detector signals simultaneously. You can
establish a high performance general chromatographic workstation with multi-channel
analysis and multi-user access.
Workstation Software - Concise Version
WS100 chromatographic workstation supports RS232 serial port or USB
interface as well as all digital signal output modes. It allows you to configure
all of your instruments and fully-automate your system. The user interface is
well-organized and straight-forward, providing you with all the most common
analytic functions as well as some frequently-used advanced functions, such
as spectrum scanning, variable wavelength scanning, etc. The streamlined
interface allows you to seamlessly integrate chromatograms into professional
presentations in the post-processing stages. This concise, but feature-rich
software will satisfy most users’ requirements.
Gradient Elution
You can manage gradient elution easily and accurately just by entering mobile
phase ratio, flow rate, and gradient time in the corresponding lists. In addition,
the software provides a pump flo w curve so you can adjust gradient settings
easily. This also allows you to follow the progress of gradient operations directly.
Spectrum Scanning
The software provides a spectrum scanning function for testing the deuterium
lamp. First, it detects the light intensity of the deuterium lamp under different
wavelengths, and then you can decide whether it is necessary to replace the
deuterium lamp based on the results. Second, you can determine the
wavelength error by the positions of two points on the chromatogram, 486 nm
and 656 nm.
Event Relay
The event relay is controlled by a temporal program to help you perform all
the required functions, e.g. switch on the conversion valve to separate and
recycle the mobile phase. Many of these functions are not available in other
popular workstation systems.
P100 lnfusion Unit UV-100 UV Detector/UV-VIS100 Detector
Users praise the P100 lnfusion Unit Wufeng Instruments’s ultra-high pressure and speedy HPLC design concepts were integrated into the upgraded UV100 UV
detector to minimize distortion due to system errors.

Fully digital exchange system to prevent the signal distortion and Deuterium lamp upgraded to international 2000-h deuterium lamp
interference that results from multiple analog-digital conversions with longer service life and higher sensitivity. Wavelength range is
with other common ultraviolet absorption detectors. 190 - 680 nm.

Recently introduced tungsten lamp option

covers the visible light region.
The unique check valve design employs an integrated structure composed of a valve core, a gem ball seat, and a gem ball. Flow cell designed with dual parallel conical bores, Wavelength range is 190 - 900 nm.
The structure is simple and provides excellent leak resistance. over conventional devices.
Minimum detectable concentration is 3x10-9
g/ml, for excellent detection sensitivity.
The pump has been upgraded, and the piston rod can be retracted automatically so you to replace the piston seal easily
without opening the instrument enclosure.
Flow cell D2 lamp

The piston seal and the check valve core of the pump can be easily checked and repaired, facilitating product maintenance
and self-service. Detector
Less noise and higher reliability.

Extended warranty service and more comprehensive after-sales service to assist with your analysis. widely used for HPLC analysis. The detector is responsive to all solutes.
Some selective detectors may not be able to detect chemical substances

index detector can be used together with a Wufeng HPLC system for
applications in chemistry, petroleum engineering, pharmaceuticals, food,

Semi-PRE Pump, High-Pressure Semi-PRE Pump,

High-Pressure PRE Pump
ELSD100 Evaporative
analysis and preparation requirements. The High
Light-scattering Detector
pressure Preparation HPLC system is mainly used in The evaporative light-scattering detector is a universal detector.
General ELSD test methods can eliminate some of the problems encoun-
laboratories. tered with traditional HPLC methods. With ELSD responses are not depen-
dent on the optical properties of the samples as they are with UV and

by the sample’s functional group. The ELSD method can be used to detect
all kinds of compounds except those with low boiling points. Today we
use ELSD mainly for detecting non-UV-absorbing substances.
 Detector
Specifications of LC-100
UV Detector

Model UV-100 UV detector UV-VIS100 detector UV-100S preparative UV detector

Flow Cell Volume
Infusion Unit
8 µl 8 µl 6 µl
Light Source D2 lamp D2 lamp＋W lamp D2 lamp
Wavelength Range 190-680 nm 190-900 nm 190-680 nm
Spectrum Bandwidth 8 nm
Wavelength Accuracy ±1 nm
Wavelength Precision Below 0.1 nm
Noise ≤0.25 × 10-5AU (static)/± 1x10-5AU (dynamic, under given conditions)
Analytical Drift ≤ 0.4×10-4AU/h (static) / ≤ 2 × 10-4AU/h (dynamic, under given conditions)
Minimum Detectable 3x10-9 g/ml (naphthalene/methanol solution)
Concentration

Model P100 (Analytical)

Liquid Infusion System Dual-piston reciprocating parallel pump, equipped with a binary high-pressure mixer
Flow Rate Range 0.001-9.999ml/min RI Detector
Max Output Pressure 42 MPa
Flow Accuracy ≤ ±0.3%
Model RI 100
Flow Precision RSD<0.06%
Detection Range 0.25 - 512µRIU
Qualitative Repeatability RSD6 ≤ 0. 1% (naphthalene/methanol standards)
Cell Volume 8µL
Quantitative Repeatability RSD6 ≤ 0 .3% (naphthalene/methanol standards)
Drift 200nRIU/h
Pressure Fluctuation 0.1 Mpa
Maximum Flow Rate 10 ml/min
Power Supply 220V/110V
Linear Range ≥600µRIU
Maximum Back Pressure 50KPa
Noise ≤2.5nRIU
Temperature Control shutdown, 30-50°C (1°C each time), 77°C fuse (dual temperature control)
Response Time 0.1, 0.25, 0.5, 1.0, 1.5, 2, 3, 6 sec
Integrator Output (Sensitivity) DC 0-1V (2mV/µRIU, 8mV/µRIU)

Semi-preparation and Preparation HPLC System

ELSD
Model P100SP (40 ml) P100HSP (50 ml) P100HP (120 ml)
semi-preparation system high pressure semi-preparation system high pressure preparation system Model ELSD 100
Light Source Laser diode with correction optical mirror, 670 nm, and maximum output being less than 5mW, in compliance with
Flow Rate Range 0.01 -40 ml/min 0.01-50 ml/min 0.01-120 ml/min FCC safety standards
Prsssure Range 0-25 Mpa 0-42 Mpa 0-42 Mpa
Detection Element Silicon photoelectric diode
Flow Accuracy ± 2% ± 2% ± 2%
Temperature Range Room temperature to 110°C
Flow Rate Accuracy RSD<0.1% RSD <0.2% RSD <0.2%
Nebulizer Gas Nitrogen is optimal, maximum 3.0L/min
Pressure Range 15-90 psi
Mobile Phase Flow Rate 0-5 ml/min
Analog Output 0-1 V or 0-10 mV
Communication TTL/contact closure error to stop LC pump
Parameter Use keyboard to enter operation parameters
Display LCD

Gradient System
Model
Mixing Principle
Mixing Capacity
Mixing Accuracy
Mixing Precision
Qualitative Repeatability
Quantitative Repeatability
Column Oven
LC100HP (high pressure binary) LC100LP (low-pressure quaternary gradient)
Gradient ratio controlled by two
of the switch valve Model CO100 column oven
2 types of liquid 2-4 types of liquid Temperature Control Principle Air circulation heating
± 1% ± 2% Temperature Range 5°C(above room temperature)~80°C
± 0.2% ± 0.3% Temperature Error ±2°C
RSD6 ≤ 0. 1% RSD6 ≤ 0. 2% Temperature Precision ± 0.1 °C
(naphthalene/methanol standards) (naphthalene/methanol standards)
HPLC Columns Installed 2 (250mm)
RSD6 ≤ 0. 3% RSD6 ≤ 0.4%
(naphthalene/methanol standards) (naphthalene/methanol standards) Power Supply 220V/110V
 Intelligent ARCUS 5 Auto-sampler

Arcus 5 is equipped with control software so you can operate it via PC. The user-friendly design facilitates fast easy analysis,
sample dilution and mixing, and other steps in processing.

The metering pump uses a gem piston rod, enabling the piston seals to endure more than 1 million cycles without any
degradation in measurement accuracy, which saves you the inconvenience of replacing it. In fact, under normal operating
conditions the piston seal will not require replacement for 3-5 years.

Arcus 5 is equipped with advanced self-protection capabilities, so the operator is alerted to minimize damage due to
misuse and potential errors can be prevented in a timely manner.

The 0.1 - 120µL injection volume ensures that both large and small volume samples can be injected with high precision.

Various optimizations reduce cross contamination among samples.

I & II Auto-sampler
Model ARCUS-5 Analytical (I) ARCUS-5 Preparation (II)

Sample Loading Standard tray (1) 2 ml x 54 vials

Expanded tray (2) 10 ml x 15 vials

(3) 4 ml x 35 vials

the other side; the system could automatically identify the tray type; maximum108 vials are
available (2 ml vial)

Vial Height H≤ 52mm (including septa and cap-mat)

Metering Pump Volume

Full loop injection

Injection Mode Partial loop injection
No waste injection

Automatic Protection Vial-missing alarm, sample needle fault alarm, pipeline jammed alarm, and leakage alarm

Power Supply AC 115-230V, 50/60Hz

Injection Volume Maximum volume 500ul

Full loop injection: RSD6 ≤ 0.3%

Partial loop injection: RSD6 ≤ 0.5%
System Test Quantitative
Repeatability No waste injection: RSD6 ≤ 1.0%

≤ 0.005% (designated washing procedure)

40 Mpa (conventional) 60 Mpa 40 Mpa

Patent No.: 2012.2.0010632.2 Maximum Pressure (ultra-high performance system)
2012.2.0540207.4
2011.2.0431981.7
2013SR056355
Metering Pump Precision
Pumping accuracy≤1%
 Application Examples in Various Fields
Application in Food Industry (Vitamin Analysis) Application in Natural Medicine Analysis (Panax Notoginsenoside)

Sample: Vitamin A, D and E standards Injection volume: 20μL Sample: Notoginsenoside, ginsenoside Injection volume:10 μL
HPLC column: SHODEX C18 Mobile phase: methanol Mobile phase:
HPLC column: Pntulips RSZG-C18,5um, A: Acetonitrile B: Water
Column length: 250mm Flow Rate: 1.000 mL / min
4.6 × 250mm, 5GEIC0125; Gradient mode: Time (min)
Temperature: 30 ℃ A% B%
Detection wavelength: 0 - 7.5 - 10.5 min, 326 - 264 -285 nm Temperature: 30 ℃
Flow rate: 1.0mL / min
1. Vitamin A 2. Vitamin D3 3. Vitamin E Detection 203nm
wavelength:

1. Notoginsenoside R1 2. Ginsenoside Rg1 3. Ginsenoside Re 4. Ginsenoside Rb1 5. Ginsenoside Rd

Application in Industry Product Test (Plasticizer Analysis)

Application in Feed Industry (Amino Acid Analysis)

Sample Name: plasticizer standard Peak time:

HPLC column: Pntulips BP-C18, 5um, No. Retention Chinese name English name Abbre
time (min) viation
4.6 x 250 mm
Sample: Amino acid standards Gradient mode:
Wavelength: UV 242 nm
HPLC column: EXFORMMA, 5µm 4.6 x 250mm
Temperature: 30 ℃ Flow rate: 1.0 ml/min
Injection volume: 10μL Temperature: 40 ℃
Wavelength: 254nm
Gradient mode:
Time (min) A phase: B phase:
Injection volume: 5μL
aqueous 100% acetonitrile Mobile phase: A: 0.1mo/L sodium acetate solution
0 60 40 (pH 6.5): acetonitrile = 93:7
B: = 20:80
14 42 58
35 30 70
45 0 100
55 0 100 1 aspartate, 2 glutamic, 3 serine 4 glycine 5 histidine 6 arginine 7 threonine 8 alanine 9 proline 10 tyrosine
56 60 40
11 valine 12 methionine 13 cystine 14 isoleucine 15 leucine 16 norleucine 17 phenylalanine 18 lysine

LC-100 HPLC LC-100 HPLC