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In Partial Fulfillment
of the Requirements for the
Science 10
By:
Grade 9 – Aguinaldo
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Reader/ Panelist
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ACKNOWLEDGEMENTS
The researchers would like to share their deepest gratitude for the help and
support to the following persons who in one way or another have contributed to the
Ms. Paula Marie Llido, LPT, their research adviser and science teacher, for her
The researchers’ family and friends for the help, encouragement and guidance
that made the study possible. The researchers are also thankful for supporting them
financially.
And most of all, the Almighty God, for His unwavering support and for giving the
researchers the strength and patience. From Him the researchers owe all that they have
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ABSTRACT
This study titled “Utilizing Okra (Abelmoschus esculentus) and Citrus Fruits as
Hand Sanitizer” aimed to determine the effectiveness of hand sanitizer in reducing
bacterial counts using okra and citrus fruits as main ingredients and to assess whether
the hand sanitizer is less irritating and fragrant to skin. Hand sanitizer samples were
tested by the respondents - the residents from the community of barangay Bugo and the
students of Philippine Southfield School. The researchers utilized the interview method
of data collection and used it as their research instrument. Based on the interview and
the observations of the respondents who tried the hand sanitizer, the texture of the
product was slightly sticky and just enough for their hands to handle. They also observed
that it doesn’t feel greasy on the hands and the odor of the product was soothing and not
irritating. The product is said to be successful by the people or the respondents who’ve
tested the product. It has been proven that the okra doesn’t have side effects and that
using this hand sanitizer takes lesser amount of time than hand washing, so people or
students can use this directly. It also demonstrated to be more affordable than the other
hand sanitizers because okra is cheaper than the other alternatives. Lastly, using this
product determined the efficacy in the reduction of bacterial counts on hands because of
the ethyl alcohol that was used.
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TABLE OF CONTENTS
Pages
ACKNOWLEDGMENTS …………….………………………………………………... ii
CHAPTER
I. INTRODUCTION
A. Background of the Study …………………………………….….............. 1
B. Statement of the Problem ……………………………………….............. 2
C. Significance of the Study ……………….……………………….............. 3
D. Scope and Limitation …………………………………………….............. 3
E. Definition of Terms ……………………………………………….............. 3
BIBLIOGRAPHY …………………………………………………………….... 16
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CHAPTER I – INTRODUCTION
Germs are found all over the world, in all kinds of places. They can invade plants,
animals, and people, and sometimes make people sick. The most common way germs
are spread is by the hands of the people, usually germs are transmitted from unclean
hands to food by an infected individual who didn’t wash his or her hands after doing
certain activities. The germs are then passed to those who eat the food.
Once germs invade the human bodies, they settle in for a long stay. They gather
up nutrients and energy, and can initiate in producing toxins, which are proteins that
behave or act like poisons. Toxins can cause symptoms of common infections and may
cause illnesses. Germs are often harmless but can also cause diseases such as cough,
The four major types of germs are bacteria, viruses, fungi, and protozoa. Bacteria
plants and get nutrients from their environments in order to live. A virus is an even
smaller micro-organism that can only reproduce inside a host’s living cell. Most viruses
can’t survive very long if they’re not inside a living thing like an animal or a person. Fungi
also cause diseases called mycoses. Protozoa on the other hand are unicellular
organisms that love moistness and often spread diseases over water (Brogan, 2018).
Washing the hands properly with soap and warm water is the single most
important thing an individual can do to help reduce the spread of infections (CDC, 2018)
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and help protect you, your family and those around you. The researchers came up with
this study because they want to investigate or try out another interesting product. Yes,
there are many problems out there but as they found out that one of the main problems
in the school room is the lack of alcohol or sanitizer, so they decided to make this
product because it is very handy, affordable and useful. The researchers also discovered
many ideas in the internet but this product is quite unique and one of a kind since they
will use Okra as the main ingredient. They want to show everyone that by using fruits
and vegetables, they can make a convenient, useful and an affordable product.
Thus, the researchers came up with this investigatory project to identify alternative
hand hygiene methods because hand washing facilities in the school are distant from the
students’ rooms and this study also wants to demonstrate hand sanitizer to be as or
more effective than hand washing with soap and water. Lastly, since hand sanitizer
doesn’t require the use of water, it takes lesser amount of time than hand washing which
The general objective of the study is to determine the effectiveness of the hand sanitizer.
Specifically, the study aimed to answer the following questions:
1. How effective is the hand sanitizer in reducing bacterial counts on hands and in
hydrating compared with other products?
2. Do the citrus fruits contained in the hand sanitizers make the hands fragrant?
3. Does the hand sanitizer be less irritating to skin or make the hands dry?
4. How affordable will it be compared with other hand sanitizers?
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C. Significance of the Study
The people who will benefit from the study are the students from any year level,
kindergarten to college. The teachers, faculty members and staff within the school
premises will also benefit from this study. The people who live in urban and rural areas
shall also signify this study. The objective is to help everyone in the community, spread
cleanliness around using the product. And lastly, for future researchers, to update the
result of the study for further research which can be a source of information to anyone in
need that will be applicable for their studies which resembles the topic.
The study focuses and tackles about the effectiveness of a homemade hand
sanitizer. The researchers are going to make a homemade hand sanitizer to help the
community of Barangay Bugo, especially the students, faculty, and staff of Philippine
Southfield School. This study is all about the community, the students and the people.
The study will use homemade hand sanitizer because the researchers want to help the
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E. Definition of Terms
Citrus Fruits - are known to grow in warm regions having thick rind and juicy pulp.
Ethanol - (also called ethyl alcohol, grain alcohol, drinking alcohol, or simply alcohol) is a
chemical compound, a simple alcohol with the chemical formula C. 2H. 5OH.
Hand sanitizer - also called hand antiseptic or hand rub, agent applied to the hands for
the purpose of removing common pathogens that can cause certain diseases.
Okra- a warm-season vegetable, also known as gumbo or ladies' fingers which is a good
Rural area - or countryside is a geographic area that is located outside towns and cities.
Toxins - are substances produced by plants and animals that are poisonous to humans.
Toxins also contain some medicines that are helpful in small doses, but toxic and
Urban area – is a human settlement with high population density and infrastructure of
built environment.
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CHAPTER II – REVIEW OF RELATED LITERATURE
Hand sanitizers address the challenge of improving hand hygiene within water-
waterless hand hygiene product. The health impacts of stimulating hand sanitizer as an
(Pickering et al., 2010). Alcohol use has been suggested as an option for hand hygiene.
Studies published between January 1992 and April 2002 in English and Thai, associated
The risk factors of hand hygiene dilemma were handwashing practice before
meals and after toilet use, toilet use, hygiene education, number of student toilets at the
school, number of handwash stations for students at the school, cleanliness of student
toilets, no separate toilets for girls, insufficient soap at handwash stations, inadequate
water at handwash stations, type of toilet, and occurrence of unimproved water storage
seeking to observed increase compliance in which the data were collected by directly
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Infectious diseases continue to be a dilemma on health and economic problem
critical, there has been a steady weakening in hygiene promotion, especially in the
illness, absences, and their associated costs. Most important strategies on infection
related absence in school settings. The difficulty of ensuring consistent and effective
handwashing among student populations has also been described. The purpose of the
studies was to assess the efficacy of the use of an alcohol gel hand sanitizer in the
classroom to help reduce the illness-related absentee rate for elementary students.
alcohol gel hand sanitizer is used in the classroom as part of a hand hygiene platform
Several studies have been conducted to assess and determine the antimicrobial
effectiveness of hand sanitizers alone, but very few literatures are available to assess
the difference between a number of disinfectants and hand sanitizers. Disinfectants are
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agents chemically designed with an immediate and continued activity which somehow
destroys micro-organisms to such a level directed for hygienic and surgical indications.
Sanitizers, on the other hand, are agents with an immediate activity that decrease the
Disinfectant uses a better form of alcohol (propanol) to achieve more bacterial reduction
remove detectable rhinovirus from the hands and prevent experimental rhinovirus
infection. Ethanol hand sanitizers were significantly more effective than hand washing
with soap and water. The organic acids to the ethyl alcohol provided residual virucidal
activity that continued for a few hours. Whether these treatments will reduce rhinovirus
Recent study finds that hand sanitizers could keep people healthy. The latest
hand hygiene research among young children in schools pointing to hand sanitizer,
according to a study published in a pediatric journal. The researchers found that children
who used hand sanitizer, instead of soap and water, reduced their sick days, respiratory
infections - causing runny noses or sore throat and antibiotic prescriptions. Hand
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sanitizer may differ from an antibacterial soap. It should be used with water, and is
A cross-sectional study was conducted to find out the range of germs present in
hand, and also how the students perceive hand washing. Contaminated hands play quite
microorganisms such as bacteria, virus, and fungi can be transferred to hands from
contaminated objects and surfaces people come into contact in daily life (Alwis et al.,
2012).
According to available scientific data, since the mid of 18th century, hand washing
is considered essential before any usual medical procedure and also considered to be a
crucial measure for monitoring infectious diseases (Ataee et al., 2017). It seems
reasonable to assume that handwashing with soap is also more effective in the reduction
of bacterial load compared to water alone. Future studies also could address the effects
Drying hands after washing is an important step as not drying them after washing helps
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bacteria to survive on them (Ross, 2018). Hand dryers are claimed to be less messy,
more affordable, and overall ecologically friendlier but the paper towel industry defends
the paper towel as being able to accomplish so much more than the hand dryer will ever
According to a Mayo Clinic publication from 2012, paper towels exhibited greater
expectations to hand dryers from a hygiene perspective, as dyers were less effective in
wiping bacteria off the hands. But in contrast to these findings, University of Buffalo
researchers stated just last year that high-speed hand dryers were actually more
hygienic than paper towels. The studies may differ with their results; it all comes down to
them with an alcohol-based formulation, as the preferred mean for routine hygienic hand
antisepsis if hands are not visibly soiled. It is faster, more effective, and better endured
by the hands than washing them with soap and water (WHO, 2009).
Recent study published in The Journal of Food Protection and led by researchers
at The Rollins School of Public Health at Emory University and collaborators from the
Universidad Autónoma de Nuevo León and GOJO Industries discovered that both soap
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and alcohol-based hand sanitizers (ABHS) were effective hand hygiene solutions at
reducing concentrations of bacteria. Effective hand hygiene is vital to prevent the spread
of pathogens and reduce the risk of foodborne illness. In fact, some outbreaks have
Applying hand sanitizer is a great alternative when access to soap and clean,
running water is not available. Once selecting a hand sanitizer, it should contain at least
60 percent alcohol. Non- alcohol based and sanitizers with less than 60 percent alcohol
In the present study the effectiveness of hand sanitizers both alcohol and non-
alcohol based claims to kills 99.99% microorganisms including the most resistant form.
have persistent antimicrobial activity for an extended period and claim to be effective in
microorganism eradication (Ali, 2015). Still, the efficiency of sanitizer depends on the
Citrus as part of the ingredients is a common term and genus of flowering plants
in the rue family, Rutaceae. These plants are large shrubs or small trees with spiny
shoots and alternately arranged evergreen leaves with an entire margin. Citrus fruit has
been cultivated in an ever-widening area since ancient times; the best-known examples
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are the oranges, lemons, grapefruit, and limes. The flowers are solitary or in small
corymbs, each flower 2-4 cm diameter, with five (rarely four) white petals and numerous
stamens; they are often very strongly scented. Citrus fruits are an excellent source of
fiber and vitamin C, which is a nutrient that strengthens the immune system and keeps
the skin smooth and elastic and also, these fruits are notable for their fragrance. The
juice contains a high quality of citric acid giving them a characteristic flavor (Hai, 2016).
Another ingredient needed is Okra which is rich in Vitamin A and flavonoid anti-
oxidants like beta-carotene, xanthin and lutein that help to keep us from lung and oral
cavity cancers. The folates present in okra help to reduce the occurrence of neural tube
defects in newborn babies. Consumption of okra helps to improve skin health and also
help to strengthen bones. It also takes care of the heart and the entire cardiovascular
system by helping to lessen cholesterol levels and blood pressure. It may also
study concluded that the moisturizing okra polysaccharide was compatible with the
formulations at all concentrations. Thus, the product containing moisturizing okra is safe,
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Moreover, whether it is antibacterial or antimicrobial, hand sanitizers are not and
should not be considered cleaning agents and are not meant as a replacement for soap
and water, but as a complementary practice. Sanitizers are most effective when used in
conjunction with diligent hand-washing. They play a substantial role and could be an
effective alternative to hand washing to achieve asepsis for all the health-care
professionals in outreach program, water scarcity areas, and in routine clinical practice.
Lastly, the Centers for Disease Control and Prevention, the World Health
Organization, and many other health experts promote hand hygiene as the single most
important measure in the prevention of infections. Thus, stressing proper hand hygiene
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CHAPTER III – METHODOLOGY
The materials used for the product are as follows: okra, citrus fruits peel such as
lemon peel and orange peel, ethyl alcohol, water and we need a blender, a cooking pot,
B. Procedure in Making
1. First, gather all of the ingredients, we needed a cooking pot and one liter of water
3. Third, when the okra is done cooking strain the okra to get the okra slime.
4. Fourth, we needed a blender to blend the okra slime and the citrus fruits peel.
5. Fifth, add the ethyl alcohol and the lemon or orange peel then blend it.
6. Sixth, we need a bowl and spoon to get the finish product then put it in a small
container.
C. Procedure in Testing
The researcher used an interview to know the effectiveness of the product. The
interview was used to hear what the person’s opinion and observation about the product
before and after use. The interview and the observation will help the researcher to find
out the good effects and the side effects of the product.
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CHAPTER IV – RESULTS AND DISCUSSIONS
A. Findings
TABLE 1.0
FEATURES OKRA GEL+ LEMON OKRA GEL +
IMAGE 1.0
14
The table 1.0 shows the different features of both Okra hand sanitizers using
different types of citrus fruits specifically Lemon and Orange. Based on the interview and
the observations of 3 different people the texture of both products were slightly sticky
and just enough for their hands to handle. They also observed that it doesn’t feel greasy
on the hands and the odor of the products were both soothing and not irritating. The
respondents suggest creating more of these products with a component that will make it
stickier and not that flowy so as not to waste the products if badly poured. The
respondents expect a more presentable product the next time they’ll try again to have
B. Analysis
After the observation and the interview that we conduct, the product has been
proven effective for the people who observed and tried the product. But, the respondents
suggest creating this product with a component that will make it stickier and not that
flowy. The texture of the sanitizer was slightly sticky, and the clarity or the clearness of
the product is hazy for the okra and lemon peel while the okra and orange peel is turbid.
The okra can be an alternative of aloe vera, and the fragrance of the fresh lemon that
came from the lemon peel and the sweet fragrance from the orange peel. EXPLAIN
tested the product. It has been proven that the okra (Abelmoschus esculentus) can
be an alternative to aloe vera, and it is proven that the okra doesn’t have side effects
especially to the skin. It is proven that using this hand sanitizer takes lesser amount
of time than hand washing, so people or students can use this directly. It is more
affordable than the other hand sanitizers because okra is cheaper than the other
alternatives. Using this product can reduce the bacteria counts on hands because of
the ethyl alcohol that was used. The fresh lemon and sweet orange fragrance that
CHAPTER VI – RECOMMENDATION
It has been proven that utilizing okra and citrus fruits peel as hand sanitizer is
successful. The researchers suggested the following recommendations in making this
product.
1. Although the taste and texture of okra is unique, some folks think its mild flavor
resembles that of green beans or eggplant. Those two vegetables may be
substituted for okra in many soups and stews. However, without okra's natural
thickening properties, cornstarch or flour may also have to be added.
2. Aloe vera can be the alternative to okra too; the aloe vera gel is an alternative
to the okra slime.
3. Unfortunately, lemon, orange and other citrus fruits have such a unique flavor
that there really is no good substitute. Because citrus is an acidic ingredient,
you could try substituting another acid, such as vinegar, cider vinegar or a
fruit- or herb-flavored vinegar, in equal amounts called for.
4. Try something that will make the product stickier such as glycerine.
BIBLIOGRAPHY
Brogan, RJ. (2018). What are germs? [cited July 2018]. Retrieved from:
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https://kidshealth.org/en/kids/germs.html
Centers for Disease Control (2018). Handwashing: Clean Hands Save Lives.
https://www.cdc.gov/handwashing/when-how-handwashing.html
Ware, M. (2017). Benefits and uses of Okra [cited 20 October 2017]. Available
from: https://www.medicalnewstoday.com/articles/311977.php
Thesaurus (2012). The Free Dictionary by Farlex – Citrus Fruit. Retrieved from:
https://www.thefreedictionary.com/citrus+fruit
Pickering, A. J., Boehm, A. B., Mwanjali, M., & Davis, J. (2010). Efficacy of Waterless
Hand Hygiene Compared with Handwashing with Soap: A Field Study in Dar es Salaam,
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Picheansathian, W. (2018). A systematic review on the effectiveness of alcohol‐based
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Turner, R. B., Fuls, J. L., & Rodgers, N. D. (2010). Effectiveness of Hand Sanitizers with
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drying-dilemma
Brogan, R. J. (2018). What are germs? [cited July 2018]. Retrieved from:
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18
Centers for Disease Control (2018). Handwashing: Clean Hands Save Lives. [Internet].
[cited 15 October 2018]. Available from: https://www.cdc.gov/handwashing/when-how-
handwashing.html
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https://www.medicalnewstoday.com/articles/311977.php
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PERSONAL INFORMATION
Name: Bhea Byl G. Rivera 6
EDUCATIONAL BACKGROUND 5
PERSONAL INFORMATION
Name: Maria Andrea Christabelle Y. Obnial
Date of Birth: August 25, 2003
Age: 15
Nationality: Filipino
Residence Address: Zone 4 upper Bugo Cagayan De Oro City
Contact Number: +639366593222
Email Address:
EDUCATIONAL BACKGROUND
PERSONAL INFORMATION
Name: Kelly Stephaie R. Layno
Date of Birth: April 26, 2003
Age: 15 y.o
Nationality: Filipino
Residence Address: #385 Zone 1 Upper Bantiles, Bugo, Cagayan De Oro City
Contact Number: +639756417499
Email Address:
EDUCATIONAL BACKGROUND
22
CURRICULUM VITAE
Insert picture
here
PERSONAL INFORMATION
Name: Narish Loui J. Catriz
Date of Birth: September 1,2003
Age: 15
Nationality: Filipino
Residence Address: Bugo, Trinitas subdivision, Block 12 Lot 28
Contact Number: +639362570025
Email Address:
EDUCATIONAL BACKGROUND
23