Академический Документы
Профессиональный Документы
Культура Документы
04
7, June, 2015 Dr. Menorca | Cofactors, Coenzymes and Prosthetic Groups
b. Mg in Pyruvate Kinase
o Mg chelates ATP to enzyme.
Mg, no ATP binding to enzyme.
o “Metal-bridged” (Enz–M–S).
Transcribers: DE LOS REYES, FERNANDEZ, FLORES, GUEVARRA, JAVIER, LANUZA, LIBIT, MAAÑO, MOLINA, PAGATPATAN Page1 of 12
SUBJECT
1.04 Dr. ABC XYZ | Topic
Transcribers: DE LOS REYES, FERNANDEZ, FLORES, GUEVARRA, JAVIER, LANUZA, LIBIT, MAAÑO, MOLINA, PAGATPATAN Page 2 of 12
SUBJECT
1.04 Dr. ABC XYZ | Topic
ISOMERIZATION REACTIONS
Transcribers: DE LOS REYES, FERNANDEZ, FLORES, GUEVARRA, JAVIER, LANUZA, LIBIT, MAAÑO, MOLINA, PAGATPATAN Page 3 of 12
SUBJECT
1.04 Dr. ABC XYZ | Topic
Transcribers: DE LOS REYES, FERNANDEZ, FLORES, GUEVARRA, JAVIER, LANUZA, LIBIT, MAAÑO, MOLINA, PAGATPATAN Page 4 of 12
SUBJECT
1.04 Dr. ABC XYZ | Topic
Reaction Oxidation
Dehydrogenase (Alcohol
Enzyme
Some Reactions Participated in by NAD and NADP dehydrogenase)
Reaction Redox
Enzyme NADH Dehydrogenase (Complex I) Reaction Oxidation
NAD+; NADH is the first source of
Glucose-6-phosphate
Coenzyme electron that will be passed down the
Enzyme dehydrogenase and 6-
chain.
phosphogluconate dehydrogenase
Coenzyme NADP+
Transcribers: DE LOS REYES, FERNANDEZ, FLORES, GUEVARRA, JAVIER, LANUZA, LIBIT, MAAÑO, MOLINA, PAGATPATAN Page 5 of 12
SUBJECT
1.04 Dr. ABC XYZ | Topic
Transcribers: DE LOS REYES, FERNANDEZ, FLORES, GUEVARRA, JAVIER, LANUZA, LIBIT, MAAÑO, MOLINA, PAGATPATAN Page 6 of 12
SUBJECT
1.04 Dr. ABC XYZ | Topic
Transcribers: DE LOS REYES, FERNANDEZ, FLORES, GUEVARRA, JAVIER, LANUZA, LIBIT, MAAÑO, MOLINA, PAGATPATAN Page 7 of 12
SUBJECT
1.04 Dr. ABC XYZ | Topic
Transcribers: DE LOS REYES, FERNANDEZ, FLORES, GUEVARRA, JAVIER, LANUZA, LIBIT, MAAÑO, MOLINA, PAGATPATAN Page 8 of 12
SUBJECT
1.04 Dr. ABC XYZ | Topic
THIAMIN (B1)
- PP & BIOCYTIN
-
-
- Thiazole ring is the one that is directly involved in the VITAMIN B6
transfer of ketone (or aldehyde) and decaroboxylation - Efficiently converted by the body to pyridoxal
of alpha keto acids phosphate, which is required for the synthesis,
- Carbon #2 (encircled with blue) is the main site of TPP, catabolism and interconversion of amino acids
it is CARBANIONIC due to the resonance inductive - Most versatile enzyme cofactor
effect of pyrimidine and nulceophilicity of sulfur - Vitamin B6s are readily soluble in water,
- Stable in acid, unstable in alkali
Reactions that involve TPP
- Easily destroyed with UV light (ie. sunlight)
1. Decarboxylation (Decarboxylase) - well-absorbed through the mucosa of the small
intestine
Forms of
Structure
Vitamin B6
Transcribers: DE LOS REYES, FERNANDEZ, FLORES, GUEVARRA, JAVIER, LANUZA, LIBIT, MAAÑO, MOLINA, PAGATPATAN Page 9 of 12
SUBJECT
1.04 Dr. ABC XYZ | Topic
Pyridoxamine
The Reaction:
Pyridoxal
Transcribers: DE LOS REYES, FERNANDEZ, FLORES, GUEVARRA, JAVIER, LANUZA, LIBIT, MAAÑO, MOLINA, PAGATPATAN Page 10 of 12
SUBJECT
1.04 Dr. ABC XYZ | Topic
BIOCYTIN
Biocytin
- Precursor: Biotin
- Transfer: CO2
- Occurs naturally in blood serum and urine
- The enzyme methyl malonyl CoA carboxylase
utilize this as cofactor
- It is a covalently bound prosthetic group of the c. N10C=O FH4 or N10 formyl THF
enzymes:
o pyruvate carboxylase
o acetyl CoA carboxylase
o propionyl CoA carboxylase
- End product of the proteolytic degradation of
biotin dependent carboxylases, e. g. pyruvate
carboxylase. d. N5CH= NHFH4 or N5 formimino THF
- To recycle the vitamin biotin, biocytin has to be
cleaved by the enzyme biotinidase.
- Used as neuronal tracer in brain research
(histological stain for nerve cells)
Transcribers: DE LOS REYES, FERNANDEZ, FLORES, GUEVARRA, JAVIER, LANUZA, LIBIT, MAAÑO, MOLINA, PAGATPATAN Page 11 of 12
SUBJECT
1.04 Dr. ABC XYZ | Topic
Synthesis of BPH4:
Transcribers: DE LOS REYES, FERNANDEZ, FLORES, GUEVARRA, JAVIER, LANUZA, LIBIT, MAAÑO, MOLINA, PAGATPATAN Page 12 of 12