BIOLOGY IN FOCUS

MAINTAINING A BALANCE

Chapter 1 Temperature regulation

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Student Worksheet: Graphs related to enzyme ). &/#53

activity
Study the graphs below and answer the questions that follow. Linked to
page 8
Graph 1

optimum
Rate of reaction in units of time

0 10 20 30 40 50 60
Temperature in °C

1. What property of enzymes is demonstrated by this graph? Justify your answer.

Graph 2
pepsin
rennin
salivary amylase

trypsin
Rate of reaction

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14
pH

Copyright © 2008 McGraw-Hill Australia. Permission is granted to reproduce for classroom use.
BIOLOGY IN FOCUS

1. Look at Graph 2 overleaf and identify the enzyme(s) that function best in a
medium that is:
(a) acidic
(b) neutral
(c) alkaline.
2. Write a general statement about pH and enzyme functioning.
3. Pepsin and rennin function in the stomach and trypsin functions in the small
intestine. Explain how pepsin and rennin are inactivated when they reach the
small intestine.

Graph 3

e
f enzym B
on o
en trati
c
con
Rate of reaction in units of time

her
hig

f enzyme
tion o A
o ncentra
er c
low

Concentration of substrate

1. Write a general statement about:

(a) substrate concentration and rate of enzyme reaction
(b) enzyme concentration and rate of reaction.
Justify your answers to (a) and (b) above, using evidence from Graph 3.
Present your answer in the form of a table.

Copyright © 2008 McGraw-Hill Australia. Permission is granted to reproduce for classroom use.