DAFTAR ISI

Halaman

HALAMAN JUDUL ...................................................................................... i

HALAMAN PENGESAHAN ........................................................................ ii
HALAMAN PERNYATAAN ........................................................................ iii
ABSTRAK ...................................................................................................... iv
ABSTRACT .................................................................................................... v
DAFTAR ISI .................................................................................................. vi
DAFTAR TABEL .......................................................................................... viii
DAFTAR GAMBAR ...................................................................................... ix
DAFTAR LAMPIRAN .................................................................................. x
DAFTAR SINGKATAN DAN LAMBANG ................................................ xi
BAB I PENDAHULUAN............................................................................... 1
1.1 Latar Belakang ............................................................................ 1
1.2 Rumusan Masalah ...................................................................... 3
1.3 Batasan Masalah ......................................................................... 4
1.4 Tujuan Penelitian ........................................................................ 5
1.5 Manfaat Penelitian ...................................................................... 5
BAB II TINJAUAN PUSTAKA..................................................................... 6
2.1 Inflammatory Bowel Diseases .................................................... 6
2.2 Tikus (Rattus norvergicus) Model Inflammatory Bowel
Disease ...................................................................................... 7
2.3 Indometasin ................................................................................. 9
2.4 Jejunum ....................................................................................... 11
2.5 Enzim Amilase ............................................................................ 12
2.6 Air Alkali .................................................................................... 14
BAB III KERANGKA KONSEP DAN HIPOTESIS PENELITIAN ....... 18
3.1. Kerangka Konsep ........................................................................ 18
3.2. Hipotesis Penelitian .................................................................... 20
BAB IV METODOLOGI PENELITIAN................................................ .... 22
4.1. Waktu dan Tempat Penelitian ..................................................... 22
4.2. Hipotesis Penelitian .................................................................... 22
4.2.1 Hewan Coba ...................................................................... 22
4.2.2 Alat dan Bahan Penelitian ................................................. 22
4.6.3.1 Alat Penelitian ....................................................... 22
4.6.3.2 Bahan Penelitian .................................................... 23
4.3. Sampel Penelitian ....................................................................... 23
4.4. Rancangan Penelitian .................................................................. 24
4.5. Variabel Penelitian ...................................................................... 25
4.6. Tahap Penelitian ......................................................................... 26
4.6.1 Preparasi Hewan Coba ...................................................... 26
4.6.2 Persiapan Hewan Model IBD dengan ............................... 26
4.6.3 Tata Laksana Pemberian Air Terapi Alkali ....................... 27

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 4.6.4 Pengambilan Organ Jejunum............................................. 27
4.6.4.1 Pengambilan Sampel dan Fiksasi .......................... 27
4.6.4.2 Dehidrasi dan Clearing .......................................... 28
4.6.4.3 Infiltrasi, Embedding, dan Sectioning ................... 28
4.6.5 Pewarnaan Hematoksilin-Eosin ........................................ 29
4.6.6 Pengamatan Histopatologi ................................................. 30
4.6.7 Isolasi Amilase .................................................................. 30
4.6.8 Pengukuran Aktivitas Amilase .......................................... 30
4.6.8.1 Pembuatan Kurva Standar Glukosa ....................... 30
4.6.8.2 Pengukuran Aktivitas Amilase .............................. 31
4.7. Analisis Data ............................................................................... 32
DAFTAR PUSTAKA .................................................................................... 33
LAMPIRAN .................................................................................................... 36

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