NOTE:

- Don’t forget to record all necessary results and inputs, if using instruments like analytical balance or
centrifuge don’t forget to logbook, use mask all the time, and please be guided when handling chemicals

For productive time

- Heat first water then cool. Weigh 3(10g), 3(30g), (50g) fly ash and mix in 50ml oil (don’t forget
documentation). Heat each mixture of fa+oil in hotplate w/ stirring time of 20min. When water is cool
then weigh 2.5g NaOH and mix in water then store. Centrifuge while heating the other ratio. Prepare
needed reagents and do the analysis.

Preparation of Reagents
NAOH

Heat 500ml Weigh 2.5g Add previously Transfer in 500ml Shake

Distilled H20 NaOH in small heated H20 and stir volumetric flask vigorously and
at 105degC beaker until all NaOH is and fill to the transfer in
for 5min dissolved meniscus storage bottle
PHENOLPHTHALEIN Indicator

Dissolve 1g Stir until Note: if results is not deducible then add

Phenolphthalein dissolved 1ml 0.1% w/v solution of methylene blue or
in 100ml ethyl use thymolphthalein or alkali blue 6B in
Alcohol place of phenolphthalein

95% by volume Ethyl Alcohol

Volume depends on Add .95(750ml) Mix and

trials: overall ethyl alcohol in store in
volume=3x5x50ml=750ml 0.05(750ml) H20 bottle

NOTE: Don’t forget documentation

50ml oil + 10g FA (3 replicates) Stir for 20 min Centrifuge

Decant in Yakult and
w/ temp of 20-30min
50ml oil + 30g FA (3 replicates) transfer small portion
105degC in at max
for FFA analysis
hotplate rpm
50ml oil + 50g FA (3 replicates)

FFA Analysis

Weigh in analytical Add 50ml ethyl Heat at 100degC Endpoint is

balance 1 test for 1 alcohol and 1ml of for 5min then slightly pink and
sample of 3-5g oil phenolphthalein titrate hot as color persists
indicator possible less than 1-2min