MRC-Holland

Description version 05; 5 October 2015

SALSA® MLPA® EK1 and EK5 Reagent Kits

To be used in combination with SALSA® (MS-)MLPA® Probemix

Catalogue numbers:
• EK1-FAM: SALSA MLPA EK1 reagent kit 100 rxn – FAM
• EK1-Cy5.0: SALSA MLPA EK1 reagent kit 100 rxn – Cy5.0
• EK5-FAM: SALSA MLPA EK5 reagent kit 500 rxn – FAM
• EK5-Cy5.0: SALSA MLPA EK5 reagent kit 500 rxn – Cy5.0

Precautions and warnings: For professional use only. Always consult the most recent probemix product
description AND the (MS-)MLPA General Protocol before use: www.mlpa.com. It is the responsibility of the
user to be aware of the latest scientific knowledge of the application before drawing any conclusions from
findings generated with this product.

Intended use: The SALSA® MLPA® reagent kits are an accessory to SALSA® MLPA® probemixes intended
for in vitro diagnostic (IVD)1 or research use only (RUO) purposes aimed at detecting and quantifying nucleic
acid sequences, as specified in the SALSA® MLPA® probemix Product Description. SALSA® MLPA® reagent
kits must be used in combination with (MS-)MLPA probemixes.
1
Please note that this reagent kit in combination with SALSA® MLPA® probemixes is for In Vitro Diagnostic use (IVD)
in the countries specified on the Certificate of Analysis of the corresponding probemix. In all other countries, the
product is for Research Use Only (RUO).

SALSA MLPA EK1 and EK5 reagent kits contents:

Reagent kit Volumes
Ingredients
component EK1 EK5
SALSA MLPA Buffer
1 180 µl 5×180 µl KCl, Tris-HCl, EDTA, PEG-6000, oligonucleotide
(yellow cap)
SALSA Ligase-65 Glycerol, EDTA, Beta-Mercaptoethanol, KCl, Tris-HCl, nonionic
2 115 µl 5×115 µl
(green cap) detergent, Ligase-65 enzyme (bacterial origin)
Ligase Buffer A
3 360 µl 5×360 µl Coenzyme NAD (bacterial origin)
(transparent cap)
Ligase Buffer B
4 360 µl 5×360 µl Tris-HCl, MgCl2, non-ionic detergent
(white cap)
SALSA PCR Primer Synthetic oligonucleotides with fluorescent dye (FAM or Cy5), dNTPs,
5 240 µl 5×240 µl
Mix (brown cap) Tris-HCl, KCl, EDTA, nonionic detergent
SALSA Polymerase Glycerol, non-ionic detergents, EDTA, DTT, KCl, Tris-HCl, Polymerase
6 65 µl 5×65 µl
(orange cap) enzyme (bacterial origin)
7 (MS-)MLPA General protocol
None of these ingredients are derived from humans, animals or pathogenic bacteria. Based on the concentrations
present, none of the ingredients are hazardous as defined in the Hazard Communication Standard. An MSDS is not
required for these products: none of the preparations contain dangerous substances (as per directive 67/548/EEC &
amendments) at concentrations requiring distribution of MSDS (as per directives 1999/45/EEC, 2001/58/EEC). A
statement ‘MSDS not required’ is available on our website (www.mlpa.com).

Storage and stability: All components of the SALSA® MLPA® reagent kit must be stored directly upon
arrival at -15 °C to -25 °C in the dark and in the original package. When stored under the recommended
conditions, a shelf life of at least 1 year is guaranteed. Check the vial label on each individual reagent for the
expiry date. Reagents should not be exposed to more than 25 freeze-thaw cycles.

Materials required but not provided: 1) SALSA MLPA Probemix, the corresponding Product Description,
and the (MS-)MLPA General Protocol. A complete overview of all SALSA MLPA probemixes offered by MRC-
Holland is available on our website (www.mlpa.com). 2) Laboratory equipment, as specified in the (MS-)
MLPA General protocol, is needed to perform the MLPA reaction. 3) Coffalyser.Net software in combination
with the appropriate lot-specific MLPA Coffalyser sheet must be used for data analysis, freely downloadable
at www.mlpa.com.

SALSA MLPA EK1 and EK5 reagent kits page 1 of 2

 MRC-Holland
Description version 05; 5 October 2015

MLPA technique: The principles of the (MS-)MLPA technique (Nygren et al. 2005, Schouten et al. 2002)
are described in the (MS-)MLPA General Protocol (www.mlpa.com).

Related SALSA® MLPA® products

• EK20-FAM: EK20 reagent kit for 2000 reactions. Only available with FAM labelled primers. For high
throughput MLPA analysis.

Implemented changes in the product description

Version 5
- Description restructured.
- Intended use changed.
- Various textual changes.
- IVD symbol removed.

More information: www.mlpa.com

MRC-Holland bv; Willem Schoutenstraat 1
1057 DL, Amsterdam, The Netherlands
E-mail info@mlpa.com (information & technical questions); order@mlpa.com (orders)
Phone +31 888 657 200

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