28/04/2019 Advances in HPLC Stationary phases in Zirconia HPLC columns

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POSTED ON DECEMBER 6, 2013 BY DR. DEEPAK BHANOT 3 COMMENTS

Advances in HPLC Stationary

phases: Zirconia HPLC columns
Silica-based HPLC columns have remained workhorses in HPLC laboratories. Over the years with
advances in particle technologies size reduction has been possible from 5μm to sub- 2μm which
led to development of UHPLC. Further introduction of Core Shell Technologies and monolithic
columns extended the range of applications and contributed to larger sample throughputs.

Properties of
Zirconia
supports
The main limitation of silica columns
is availability of pH range between 2
and 8 and temperature to ambient.
On the other hand Zirconia supports
exhibits operational stability over
the entire pH range and working
Monodisperse, Spherical and Nonporous Zirconia POPULAR LATEST
temperatures up to 200°C.
Particles
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Zirconia or ZrO2 is an oxide in which the Zr atom acts as a Lewis acid i.e. – it accepts electron pair
due to prescence of empty orbital. This feature provides enhanced selectivity over conventional
silica columns.
TOPICS

Adsorption capacity AAS (53)

Analytical Chemistry (3)

Adsorption capacity is larger in comparison to silica packings
Calculations (5)

Chemical resistance
Chemical (2)

Chromatography (9)
High resistance to acidic and basic solutions. Retention of basic and acidic analytes can be nely
Environmental Analysis (2)
controlled by changing the pH of medium over the entire available range.
Featured (2)

Types of phases Free e-courses (2)

Gas Chromatography (55)

Four surface chemistries based on surface activities are available which greatly extend selectivity
and retention of for improved separations General Topics (140)

Guest Posts (27)

Polybutadiene coated Zirconia HPLC (60)

The phase is similar to reverse phase using C18 silica but has following advantages: ICP Techniques (13)

Jobs (1)
Available pH range 1 – 13
Lab Bytes (17)
 Temperature stability up to 200°C
Less hydrophobic than C18 and is suitable for separations involving amines and bases Lab Management (95)

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28/04/2019 Advances in HPLC Stationary phases in Zirconia HPLC columns

Zirconia-C18
Laboratory Insurance (8)

Laboratory Safety (13)

Porous carbon clad zirconia particles covalently modi ed with C18 groups.
Method Validation (1)
Selectivity is rather di erent from conventional C18 columns
Can be used over the entire pH range (1 – 14) alongwith high temperature capabilities (up to Microbiology (14)
200°C) News (3)

Paper Chromatography (10)

Zirconia – polystyrene pH Measurement (3)

Porous Zirconia particles are modi ed with cross-linked polystyrene. Preformulation Studies (3)
Tolerate strongly aqueous solutions up (up to 100% pure water)
Quality System (9)
Operational pH range 1 – 13
Useful for separation of hydrophobic compounds and amines Separation Science (11)
No requirement for ion pairing requirement
Spectroscopy (80)
Reduced tailing of basic compounds in comparison to silica-based packings
Provides mixed mode Reverse phase and Ion exchange separations Stability studies (8)

Training (21)

Zirconia-Carbon Weighing (3)

Porous carbon clad Zirconia particles provide enhanced retention of polar compounds
Highly hydrophilic surface
Useful for separation of isomers and diastereomers
Good retention of fused – ring aromatic compounds

Column care
Contaminated columns can deteriorate the peak shape, e ect retention times, lead to high
backpressure and increase baseline noise. Use of concentrations above 25 mM phosphate,
acetate, carbonate/bicarbonate bu ers and salts should be avoided. Anions such as carboxylic
acids, uoride and phosphate strongly absorb on Zirconia columns. The recommended steps for
column regeneration are

1. Flush with a 20:80 mixture of acetonitrile and pH 10 ammonium hydroxide for 50 column
volumes followed by 10 column volumes of water
2. Repeat with 20:80 mixture of acetonitrile and 0.1 M nitric acid for same column volumes
3. Flush with pure methanol, acetonitrile, isopropanol or tetrahydrofuran for 20 column
volumes. Zirconia HPLC columns will require the same solvents with at least 20% THF

Storage
Zirconia HPLC columns should not be stored in phosphate bu er. The recommended
storage procedure is
Overnight – 10 volumes of organic modi er/water
Long-term storage – 10 volumes of pH 10 ammonium hydroxide followed by 10 volumes of
50: 50 organic modi er/water

We have seen that zirconia-based reverse phases o er a great choice of selectivity and retention.
Additional bene ts of column chemistries, pH and temperature extremes contribute to versatility
of applications. A subsequent article on monolith stationary phase packing will introduce you to
further advances in stationary phases.

Please share your views and leave comments on the article

Filed Under: HPLC

About Dr. Deepak Bhanot

Dr Deepak Bhanot is a seasoned professional having nearly 30 years expertise
beginning from sales and product support of analytical instruments. After
completing his graduation and post graduation from Delhi University and IIT
Delhi he went on to Loughborough University of Technology, UK for doctorate research in
analytical chemistry. His mission is to develop training programs on analytical techniques and

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28/04/2019 Advances in HPLC Stationary phases in Zirconia HPLC columns
share his experiences with broad spectrum of users ranging from professionals engaged in
analytical development and research as well as young enthusiasts fresh from academics who
wish to embark upon a career in analytical industry.

Comments

Meinster Kodjo Eduafo says:

February 25, 2017 at 2:51 am

Dear Dr. Deepak

I think this is a great breakthrough for HPLC analysis. please can send me a
quotation for each of them. I will be very grateful. Thank you very much for this
insight.

Best regards

Reply

Dr. Deepak Bhanot says:

February 25, 2017 at 9:49 am

Please specify your requirement.You may subscribe to our on- line training
programs but let me clarify that we are not a supplier of columns or any
chromatographic consumables.

Reply

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