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Concept:
Glucose is a reducing sugar. It has reducing power and can react with oxidising agents.
Introduction
Benedict’s reagent is often used to test for the presence of reducing sugar (e.g. glucose) in
solution by the formation of a brick-red precipitate. However, it is not very accurate
when using Benedict’s test to conduct quantitative analysis of glucose.
purple colourless
6. Repeat (2) to (5) with other glucose solutions of known concentration until all are
finished.
7. Record your results, and share among the class.
8. Calculate the class average results and record in a table.
9. Plot the class average results on a graph paper to make a standard curve.
10. Repeat (2) to (5) for glucose solutions (A, B and C) of unknown concentration.
11. Use the standard curve to estimate the glucose concentration of solutions A, B and C.
2
Questions for discussion
1. What is the purpose of pooling the class results for plotting the standard curve?
2. Suggest how you could improve the experiment.
3. Suggest any possible reasons if your result is different from other groups.
Further investigation
Design an investigation to find out the glucose content of a variety of “health” drinks, and
compare your results with the glucose content marked in the food labels. The results will be
affected by the presence of vitamin C in some drinks, as it may cause the decolourisation of
potassium permanganate solution to take place more quickly. Discuss the experimental
design with your classmate before carrying out the investigation. Write a report of your
investigation.
Reference:
Science and Plants for Schools (2007) Estimating glucose concentration in solution. SAPS.