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Product composition:The analytical part (host), operation part (computer system), the
result output part (printer), accessories and consumables.
Product applicable scope: used for quantitative analysis of serum, plasma, urine,
cerebrospinal fluid and other clinical chemical constituents of sample.
②Upper cover
③ ④
⑤ S injection pump
⑥ R2 injection Pump
⑦ R1 injection pump
①②③④ ⑤ ⑥⑦
1
1-1-4 The back of analyzer
① Power entrance
② RS232 interface
④ cooling fan
⑧ R1 reagent disk
⑾ sample disk
⒀ R2 stirring mechanism
⒁ R2 reagent disk
2
1-1-6 The Right of Analyzer
④power indicator(red)
①② ③ ④
3
1.2 Analytical Unit Composition
CS-400 auto-chemistry analyzer working speed means the one at which it reaches
constant speed 400 tests / hour of single / double-reagent item, whose working period is 9
seconds. Instrument overall structure adopts the "4 -disk + three-probe + two-stirring rod",
specifically, a sample disk, one reaction disk, two reagent disks, 2 reagent probes for
adding R1 and R2 respectively, a sample probe for sampling, 2 stirring rods for mixing
R1,R2 respectively. "Grating + diode array" approach is adopted in optical measurement
mechanism for real-time optical collection of reaction cup. The rinsing mechanism 7-stop
11-step automatically rinsing the reaction cup is carried out in test process.
QC position: 8
Barcode window: 1
4
1-2-3 Reagent Unit
Reagent position:45×2
cup
Incubation bath
5
1-2-5 Probe and Stirring Unit
Stirring mechanism probe mechanism
Probe mechanism:3
Stirring mechanism:2
① ② ③
① ISE unit:3(SIP,DIL,IS)
6
1-2-7 Control unit 1
① ②
① circuit panel boxes: 6 panels
+12V,-12V(simulation)
+12V(lamp)
+5V(digital circuit)
+24V(motor, valve)
③ ④ ⑤ ⑥ ⑦ ⑧ ⑨
③ Halogen Lamp Power Interface
⑨ fan interface
7
1-2-8 Control unit 2
① ②
+5 V panel power
cooler
Cooler display:
AC circuit breaker: 5
Fan (4A)
2 Heater (10A)
Isolating transformers
Arrester panel
8
1.3 Function Overview
2. 4 times water blank measure is implemented after the fifth time rinsing of 7 times
automatically rinsing of reaction cup.
3. Sample assimilates quantitive sample when it descents to sample disk after the
sample disk rotates to designated sampling position.
4. After 7-stop 11-step cleansing, reaction cup stops at the sampling position, and
sample probe rotates to reaction disk and descends to reaction cup to discharge
it, and sampling finished.
6. R1 reagent probe rotates to reaction disk and discharges reagent R1 when the
reaction cup finishing sampling rotates to R1sampling position.
10. The R2 reagent probe discharges the R2 into reaction cup when it rotates to
reaction disk after reaction cup rotates to R2 sampling position.
11. Finishing sampling R2 reagent, reaction cup is stirred after its one circle (2
patches) rotation.
12. Reaction cup carries out the collection of absorbance data when it passes the
optical unit in every period.
13. The process of sampling R3,R4 reagent and sampling R1, R2 reagent.(the
same system for R1、R4,the same system for R2、R3)
9
Table 3-1-1 Main Function of Each Unit
Sample probe unit Execute sample assimilation and discharge of all biochemical
items and ISE items
Sample disk unit Total 115 sample positions for carrying all test samples,
standard solution and QC liquid.
Reagent R1 disk total 115 reagent positions for carrying R1, R4 test reagent
unit and detergent
Reagent R2 disk total 45 reagent positions for carrying R1, R3 test reagent and
unit detergent
Reaction disk unit Total 120 reaction cups used as container of reaction and
colorimetry test.
Reagent R1 stirring Stirring when reagent R1, R4are added into reaction cup.
unit
Reagent R2 stirring Stirring when reagent R2、R3 are added into reaction cup.
unit
barcode Total 3 for scanning reagent bottles in the two reagent disk
and sample cups in sample disk.
10
Chapter 2 Instrument Installation
To make sure the space of maintenance, operation and repair, please follow the instruction
as below:
● Space between left (right) side of analyzer and the wall should ≥50cm
● Space between rear board of analyzer and the wall should ≥50cm
● Space in front of analyzer should≥100cm
● Make sure there is enough space for waste device and purified water
equipment.
● Power: 2000 VA
Instrument is equipped with a three core electrical wire, red wire is live line, blue wire is zero line,
and yellow green wire is ground lead as figure 1 shows.
Figure 1 i
A well grounded power supply socket is a must. (Socket at least with one 20 A and three 5A).
Large electrical appliance such as air condition, refrigerator, even cannot use the same
electrical wire as analyzer.
11
! Warning:
△
● Environment should be with no dust, mechanical vibration, and noise source and
power interference
● Do not put the analyzer in the vicinity of brush motor, flicker fluorescent tube and
other constant on-off electrical equipment.
Hard and flat enough the ground should be to stand the instrument.
● Avoid direct sunlight, do not put the analyzer in front of heat source and wind source
! warning:
△
¾ Normal running and accuracy of result can not be guaranteed if instrument works beyond
the requirements mentioned above. Please use air conditioner if the temperature or
humidity can not meet the requirement above.
¾ The heat generated in the work process by the instrument will be emitted the rear of the
instrument, so good ventilation should be kept well and ventilation equipment can be
adopted if necessary, but direct air current is avoided, or inaccuracy of instrument test may
be caused.
4.2 2.4 Purified water equipment Requirement:
① water should be obtained from tap water pipe
② water conductivity should within 1uS/cm
¾ Make sure the installation place, space, electrical environment, installation room
temperature and purified water equipment can conform to requirements
¾ Make sure instrument installation tools needed are complete and reagent and QC liquid are
enough.
¾ Please check the prepared items according to packing list when open the package; please
write them down on the check report if any missing.
12
¾ Place instrument in applicable position, and mount with computer host, display and printer.
¾ Connect water supply and waste liquid outlet equipment.
¾ Adjust instrument level, and check whether the injection pump wires are loose or not after
open the left and right cover board of instrument.
¾ Infuse CS-alkaline detergent into instrument rinsing box, and infuse CS-anti-bacterial
detergent into the 45th position of R1, R2 reagent disks.
¾ Replenish cooling system water tank with purified water.
(a)Switch off the main power
(c)Unplug the cork of the two hoses connecting to cooling system water tank as figure 3 shows :
13
(d)Infuse purified water into low water level hose till the purified water flows out of the
high level hose.
(e)Switch on the main power. After several minutes, continue to infuse purified water
into low water level hose till the purified water flows out of the high level hose again,
requiring 3L water.
(f)Replug the rubber cork and mount the left front cover board of the instrument.
Executing irrigation cleaning liquid pipeline exhaust is infusing cleaning liquid into
pipeline to expel air in pipeline.
Make sure reagent probe is right above reaction cup, rinsing groove and reagent bottle.
Place a standard cup at position C8 in the sample disk outer track, middle track and
inner track respectively, and make sure the sample probe is above reaction cup, rinsing
groove, standard cup by implementing sample probe horizontal check.
14
(e)Stirring rod horizontal check
In order to make sure the stirring rod is above the reaction cup, rinsing groove.
Execute 20 times mechanical movement checks to make sure whether the washing
block of rinsing mechanism nozzle abrases the reaction cup or not and each
mechanism runs normally or not.
Select rinsing reaction cup in “Maintenance” interface, and execute rinsing reaction cup
+ ISE if ISE equipment is collocated.
Light quantity result should be attached to installation check report with its value no
more than 18000.
No. 1 cup blank value should be within 18000, and 2-120 reaction cup check value
should be within 18000 ±800.
Edit chemical parameters; register reagent info.; testing rate assay ALT, point assay,
two-point rate BUN; calculate the difference of parameter and the result of test should be
attached to installation check report.
15
Chapter 3 Performance and Test Flow
Wavelength
±2nm
precision
characteristics Reaction
37℃±0.1℃
temperature
Measuring
Rate assay ,end-point assay, 2-point assay.
method
16
Sample
2~35ul,0.1μl Incremental
volume
Remaining
sample More than 100μl
volume
Reagent
Reagent system 20~350ul,1μl (incremental)
volume
Reagent
bottle 20mL 、70mL
specification
Reagent
remaining Reagent liquid volume: more than 3 ml
volume
17
Reagent R1、R2: using special probe respectively with liquid
probe level detector and bump-proof function.
Reagent
Inner and outer wall rinsing
probe rinsing
Reagent
storage All reagent should be stored at 5℃~15℃
temperature
Reaction cup
Discrete
mode
Reaction cup
6mm
optical path
Reaction cup
6 sets, 20 for each,total 120.
quantity
Reaction
liquid 150~450ul
volume
Absorbance
0~3.3ABS
range
QC QC interval, monthly QC
18
Stirring
Separately stirring after adding reagent
system
Connecting
LIS/HIS LIS/HIS system available
system
Water
25L/h
consumption
Note:Due to the different specific conditions, sometimes equipment processing capacity will be
lower than 400 tests / hour.
19
Stop automatically
20
Rinse reaction cup
1min
plus
21s
Tested completely
stir
Add reagent 4
Total time for testing the 1st sample: 18min
stir
13min 3os
3min plus27s
Add reagent 3
9s for one additional sample
stir
18s
9min 18s
stir
15min
Add reagent 1
9s
Add sample
Assimilate water
Typical test flow
3min
figure 3-1 test flow Rinse reaction cup
Test Flow
Initial running(Reesetting)
Start
3.2.1
3.2
3.2.2 Test Flow Instruction
c.Descend till the sample probe point into liquid level more than 2mm
e.Rise from sample test tube and rotate to above reaction disk
c. Descend till the reagent probe point into liquid level more than 2mm
e. Rise from sample test tube and rotate to above reaction disk
The same to R1
21
3.2.2.5 Movement and time sequence of reaction disk
A track includes total 120 reaction cups in reaction disk, and rotates in a fixed way when
testing. The reaction cup always rotates and stops 3 times counterclockwise, total 22+37
+2=61 (rotation and stop sequence 22-37-2-)patches, in every working period, 9
seconds elapsed. 122 patches are passed in two working periods within 18 seconds.
Photoelectric
detection
R1, R4 Probe
60 R1 Pipetting
61 R4 Pipetting
Reference position
number
Reagent probe 2.3 Stirring rod
Outer circle figure: No. of reaction;inner circle figure: No. of mechanism position;
Sample position: No. 1 position; reagent 1,4 probes: No.60,61 position; stirring at
NO.62,63 position; reagent 2,3 probes: No. 31 position, stirring: No. 33 position; reaction
cup rinsing mechanism: No. 101、103、105、107、109、117、11 position.
Reaction cup stops at No. 101 position after reaction disk resetting. The sequence of
reaction cup rinsing and sampling:
22
3.2.3 Reaction Cup Rinsing Movement Sequence
Rotati
Rotationa 测4 4 次杯空白
times (1 次停止,
cell blank
l l 次通过)
measurement.
direction
1 stop, 3 pass.
f
Above figure shows that seven steps are needed when rinsing reaction cuvette. (four times
cell blank test is added) , therefore, to finish rinsing one reaction cuvette, 11 steps are
needed:
23
3.2.4 Optical measurement movement sequence
Photometry in the entire process is adopted. In 15-minute reaction time, the continuous
determination of the absorbance of reaction solution is carried out. Reaction disk rotates 1
plus 2 pitches, about 18 seconds, absorbance values are measured out when the 120
reaction cups passing optical axis of the photometer one by one.
Each reaction Cup in 3-minute reaction time was measured 10 times (10 photometric
points), 4-minute reaction time was measured 13 times (13 photometric points), 5-minute
reaction time was measured 16 times (16 photometric points), 10-minute reaction time was
measured 33 times (33 photometric points), 15-minute reaction time was measured 49
times (49 photometric points).
Detector
(340-750nm)
12 fixed
wavelength
Light starting from the light source was focused by the lens, and passed the reaction cup
first, and then was disparted by concave grating. After spectrophotometry, each
wavelength were received by 12 fixed photoelectric sensor simultaneously, and were
amplified 12 amplifier, after Log transformation to derive the rate of change of absorbance
or absorbance. When dual-wavelength testing is used, the concentration value is
calculated by the difference of the main and sub-wavelength absorbance or that of
absorbance change rate, and therefore dual-wavelength testing can not only compensate
the blood lipid, hemolytic, jaundice sample test, but also compensate on the result
impacted by voltage changes, so that measurement is more accurate, more stable.
24
Chapter 4. Module Introduction
Sample/reagent probe unit includes sample probe, No. 1 sample probe(R1) and No.2
sample probe (R2), which are called 3-probe component.
Sample probe can realize the assimilation from the sample test tube and sampling into
reaction cup. No. 1 sample probe(R1) and No.2 sample probe (R2) can realize the
assimilation from the reagent bottle and adding reagent into reaction cup.
In addition, main function of 3-probe component: liquid level detecting and bump-proof in
movement process, sample probe block detecting function.
Probe drive mechanism plays a key role of reagents and samples adding. The way of
probe are only up-down and circular moving, so two step motors are necessary to drive.
Probe up-down
weight block
Probe up-down light
sensor block tablet
Probe up-down
guide slider
Probe up-down step
motor
Probe up-down
gear belt
Reagent 1, reagent 2 and sample probe drive mechanism in addition to different probe
turning angles, namely the corner mechanical limit block tablets different, the other
structures are identical.
25
figure 4-2 Probe body and rotating body
Guide
movements
long hole
Up-down
motor
mounting
Lower rotating axle hole
center
Probe rotating
knight head
Rotating probe drive ratio 12:34, using 0.9° stepper motor and 8 segment controller.
Control accuracy can achieve 0.0398°.
Up-down main gear driver diameter is 19.1mm, the 60mm for the perimeter, also using 0.9°
stepper motor and 8 segment controller. Up-down control accuracy can achieve
0.01875mm.
26
4.4 Rotating mechanism unit
The main function of rotating mechanism is bearing of the sample warehouse, reagent
warehouse and reaction disk, and drive it to rotate, so that sample, reagent carried in
reaction cup rotate to the designated location to finish sampling, mixing and other work.
Reagent disk 1, reagent disk 2, the sample disk and reaction disk mechanisms are classified
as turntable mechanism. Meeting the requirements of functionality and performance
simultaneously, in order to improve the craftwork of product, the four disks are designed as the
same frame structure.
1, the sample turntable: The sample storehouse, disk rotating bracket, step motor-driven
components
2, reagent turntable: The reagents storehouse, disk rotating bracket, step motor-driven
components
3, the reaction disk turntable: reaction plate, the reaction cup, incubation bath, disk rotating
bracket, step motor-driven components
S/Driving seat
Reagent disc 1 and disc 2 are same the structure , and the sample disk drive structure and
the drive transmission ratio are the same with reagent disc.
27
Figure 4-4 sample/reagent disk motor driver configuration
Driven gear
Drive gear belt
System transmission ratio is 10:1.Because of using 0.9 °step motor with 8 segment driver
circuit, the wheel rotation accuracy can achieve 0.01125 °.
28
Figure 4-6 sample/reagent disk transmission code disk configuration
The only difference of drive structure of reagent and sample disk is the code disk.
R1 and R2 with two refrigerated reagent disk, adopting semiconductor refrigeration, the
temperature maintains at 6 degrees -10 degrees, 45 reagent positions for each reagent
disk respectively(the 45th fixed position for placing phosphor-free CS-anti-bacterial
cleaning liquid in each disk)
29
4.5.2 cooling system configuration and installation
Figure 4-7 reagent cooling storehouse reagent box rack assembly configuration
Equipped with
temperature-keeping reagent
cooling storehouse
Reagent box
Barcode reader
scanning window
30
Figure 4-8 reaction disk assembly configuration
Gear drive is adopted to reaction disk due to the relatively high positioning precision.
Fixing pin
Colorimetric cup
rotating rack
Driving gear
Driven gear
motor vibrator
cuvette
cuvette install pin
Locking screw of
colorimetric cup rack
6 sets of colorimetric
31
Figure 4-10 Incubation bath components configuration
R1 stirring rod
rinsing bath
Optical
window
Spilling outlet of
Circular constant incubation bath
temperature water
outlet Circular constant
temperature water
inlet
Figure 4-11 reaction disk with incubation bath components assembly configuration
Phosphor-free
Incubation bath detergent inlet
Figure 4-12 reaction disk and optical system components assembly configuration
Optical system
32
4.6 Stirring unit
Reagent 1, reagent 2 agencies are identical in addition to the mixing angle of rotation,
namely the code disk is different.
Mechanism rotation
mechanical limit
Stirring mechanism
up-down motor
Stirring mechanism
up-down slider
Rotating mechanism of stirring and rotating arm adopts the direct drive way of
step motor output axle, using 8 segment drive circuit of 0.9 ° motor, and the control
accuracy can achieve 0.1125 °. The largest angle is limited by the open angle of
rotatation code disk mechanical limit. And positioning is determined separately by
the left and right light sensors.
Stirring mechanism
up-down light Stirring mechanism
sensor up-down motor
Curve axle and curve handle drive is adopted by stirring up-down mechanism .
33
Figure 4-15 stirring up-down slider drive configuration
Up-down linear
sliding axle
Up-down
guide axle
Up-down
slider
Because of mixing body movements are used by way of the crankshaft crank,
so the movements of the positioning accuracy at different locations different.
Landing and taking-off between the location of the speed of the highest, lowest
accuracy, and precision at both ends of the highest, the lowest speed.
Electrical axis from the axis of the slider bearings the size of 16.5mm, crank in
horizontal position, the landing position accuracy of 0.032mm.
Due to curve axle and curve handle drive is adopted by stirring up-down
mechanism, the up-down positioning precision are different at different position.
The speed is the highest and precision is lowest at the middle, however the
precision is highest and speed is lowest at the two ends.
The size of Motor axis from the axis of the slider bearings is 16.5mm, when
curve handle locates horizontally; the up-down position precision is 0.032mm.
Up-down
slider
Up-down
slider
34
4.7 Colorimetric cup rinsing mechanism
Up-down
step motor Up-down slider
35
4.9 Rack
Rack includes the main rack, electrical, gas liquid valve and water tanks and
other racks.
Isolating
standing
board
Adjusting foot
All-directional
caster
Isolated
transformer
Power switch
rack
Electric rack is mainly consisted of control panel box, power rack and
waterproof board and such parts.
36
Figure 4-20 gas、 liquid valve rack configuration
Water inlet
Vacuum pump
Figure 4-21 Valve rack, gear pump rack and the main rack assembly.
37
figure 4-22 Water-in tank unit configuration
Pressure gauge
Vacuum exhausting
tank
Magnetic pump
CS-400 adopts advanced flat field grating photometer. Concave holographic grating
photometer is today's domestic and foreign advanced optical system with simple
structure, high optical efficiency, and signal to noise ratio and measurement speed
of machine have greatly been improved, compact photometer dimension, stable
performance, long life, and such highlighting advantage, achieving the requirements
such as multi-wavelength, multi-item simultaneously testing at high speed,
multi-wavelength (12) simultaneously collecting signals, a relatively large aperture,
good imaging quality and post-spectrophotometry.
Photoelectirc
cable array Flat field grating
Log applifier
38
Chapter 5. Instrument liquid and Gas Line.
The CS400 liquid line system can be divided into five parts: water inlet tank,
refrigeration, 37 degrees centigrade temperature, colorimetric cup cleaning, the
inner and outer arms and stirring rod cleaning.
2. Sampling subsystem uses three probes plus two stirring rods and three
injectors. The sampling injector uses 100uL, and the two reagent
injectors use 500uL.
3. The inner and outer wall cleaning of three probes and two stirring rods
uses barotropic driving.
4. Cleaning bath: five cleaning bathes plus waste liquor in the reagent
storehouse, together with the flooding waste liquor in the reaction disk
are seven kinds of routine waste liquor.
5. The reaction cup cleaning uses the way named 7-stop 11-step.
6. Water supply: uses the special outboard water-supply equipment and
the special water-supplying machine.
7. Waste liquor: use plastic hollow main pipe whose inside diameter is 20
to 30 mm, and the wall thickness is 3 to 5 mm. The installation of the
main waste liquor pipe is height limited, and it requires the height is
helpful to the waste liquor entering the low concentration liquid buffer
vessel by its self-weight. As to the high concentration waste liquid, it is
providing liquid level sensor interface and outboard high concentration
waste liquid barrel.
8. Source of power: the power of cleaning comes from the magnetic
pump..
9. The cleaning of reaction cup should use two kinds of detergent..
10. The vacuum degree for vacuum pump assimilating is between -28k and
-35kPa.
11. The inner and outer wall cleaning of three probes uses independent
solenoid valves while the two stirring rods use one solenoid valve
together.
39
5.2 Liquid Line Principle
40
Figure 5-2 liquid line of water inlet tank
1, when the low water level float detects out the signal, open the inlet valves
SV8, and water tank begins to be infused water until the high water level float
detects out signal, then turn off SV8 to stop the water. Influent flow is as follows:
Alarm of low water influent water Alarm of high water level Stop influent
level check check water
2, when the low water level float did not detect out signal, the water tank heater
began to work, and temperature control started. Magnetic pump began to work
simultaneously.
3, Output water pressure of water tank is controlled by the magnetic pump and fixed
damper regulator. Magnetic pump head is 8 / 11 m. Control water pressure is around
0.8kgf/cm2.
4, The outlet of tank water leads directly to the cleaning and incubation bath; the
other output water through gear pump supercharger to the pressure 1kgf/cm2 to 3
line probe mechanisms to rinse inner wall and pipeline, with exhausting device of
probe liquid line.
5, SV13 valve remains closed status so as not to affect water pressure during the
normal use of instrument. Only in the implementation of the maintenance of water
tanks, open SV13 valve, at the same time, time switch of SV8 inlet valve to empty
impurities in a water tank.
6, when abnormality occurs to the high and low water level floats, possibly water
tank remains the status of inputting water. When the water tank is full of water, SV8
not shut down, tank outflow water spills out from the overflow pipe into the waste
liquid barrel through waste liquid pipe.
41
Figure 5-3 Semiconductor cooling refrigeration liquid line
1.In the system, two reagent disks as well as standard and QC sample need to be
cooled in the environment from four to ten degrees centigrade. Water is used to
exchange temperature and transfer heat.
2. the system also adopts the semiconductor refrigeration mode which is better for
environmental protection and convenient for maintenance than the traditional.
4.the refrigeration power of each module is about 80W, the total 320W. Radiation
adopts pyrotub wind-cooled heat pipe to radiate. The single power is 150W.
5.the refrigeration warehouse shell with two reagent disks adopts stainless steel
structure, and the cold water flows into it from the bottom and flows out from the top
through circumvolution circulation, thus the warehouse interlayer is full of cold water
and its temperature is even.
42
6.the refrigeration volume of sample disk is small and the refrigeration area is
formed by aluminum cylinder, so the aim of refrigeration can be achieved by
circulating stainless steel tube. The whole circulation process is finished by magnetic
pump with a head 2.7m.
7. sluice water tank can check temperature and water level. The scope of
temperature is from 5 to 15 degrees centigrade above zero.
When adding water, it should be added from the inlet tube at the bottom by filler.
About 1.5 litter is added into water tank or flowing-out water from the top of water
tank observed (the two water-changing nozzle should be above the water tank,
water-changing tube is mainly used to exhaust). Here, turn on the main power
supply and the refrigeration system starts to work. Then the water level will drop,
water should be added continuously until the exhausting tube reflows out water.
When working stably, there is no change with the water level of exhausting tube, and
the two water-changing tube plugs should be installed. If there is no leakage, the
system water changing has been finished.
When spouting the water, please turn off the main power supply, pull out the two
water-changing tube plugs and contain water with container. The total water volume
is approximately four litters.
43
Figure 5-4 constant temperature system liquid line
This system offers precise constant 37 degrees water to the incubation bath of reaction disk,
and cools the high temperature light source simultaneously. This system consists of magnetic
pumps, inlet valves, release valves, liquid level detector and temperature controller which
consists of the heater temperature
1, Open the inlet valve SV10 and turn off outlet valves SV16 to infuse water into
incubation bath, simultaneously with reagent R1 and R2 probes adding
phosphor-free anti-bacterial rinsing liquid to the incubation bath, liquid level detector
determining whether to stop water.
2, Turn off outlet valve and inlet valve, and start the water circulation magnetic
pump and temperature controller. In order to improve the adjusting performance of
the PID temperature controller in high temperature environment, the system is
added the cooling device through the water tank to get a small amount of
temperature cooled.
3, Turn off magnetic pump and temperature controller, open the drain valve SV16,
time to turn off the drain valve when the incubation bath is draining.
44
Figure 5-5 probe internal and external walls rinsing and sample probe block check liquid line.
1, Opening the valves SV1 and SV41 simultaneously can get the inner wall of
sample probe rinsed; open valve SV2 or SV3 to carry out reagent probe R1 or R2
internal wall cleaning. Probe position should be at the top of the corresponding
cleaning trough when cleaning so that waste liquid can get out of the instrument.
2、Open the valve SV4、SV5、SV9 or SV6 to rinse the external walls of sample,
reagent R1, R2 or 2 stirring rods. Each stirring rod has one corresponding valve
respectively. Fixing pressure adjusting piston is adopted to every external wall
rinsing pipeline to avoid rinsing water spilling out of rinsing bath.
3, valve SV1 pressure testing and the valve SV41 constitutes series structure, not
only completing the sample probe cleaning of the inner wall, but also completing the
block detection of probe, which does not affect the accuracy of assimilating and
discharging liquid volume of a small amount of sample.
4. The block detection must be implemented when probe is cleaned. Turn off valve
SV1 after the cleaning, Detect pressure, and probe is completely blocked or partially
blocked if the pressure value change is the same or smaller than the range value.
And previous added sample should be deserted and alarm is issued. Turn off SV1
and SV41 when probe assimilates and discharges sample.
45
Figure 5-6 Colorimetric cup rinsing liquid line
In order to achieve the cleaning effectively, colorimetric cup rinsing adopts warm
water. In order to improve cleaning speed, colorimetric cup adopts hydraulic valve
switch and vacuum liquid exhaust.
2, vacuum pump, vacuum tank and pressure detector composes vacuum source
with pressure value about -0.8kgf/cm2. Vacuum tank has some fluid, gas
separating role in order to guarantee the safety of vacuum pump besides having
stable vacuum pressure.
46
3, There is a device on the vacuum tank to eliminate air bubbles with liquid, gas
separating bottle.
5, when the colorimetric cup cleaning mechanism descends, turn off valves SV30
and SV31 first, and then open the valve SV21. It tarts assimilating sample under the
vacuum pressure, and the liquid of colorimetric cup will be discharged after a short
period of time when the rinsing mechanism arrives at the bottom of Colorimetric
Cup.
6, cleaning mechanism begins to add cleaning solution and ionized water, turn off
valve SV21 after the addition is completed, and then open valves SV30 and SV31.
At this time, the waste liquid discharged into isolating bottle outflows by its gravity.
7, cleaning liquid and ionized water adding is completed by the five valves SV42,
SV43, SV14, SV15 and SV11 which are timed. Because the vacuum liquid
discharging begins to work simultaneously when adding liquid to discharge
redundant liquid, the liquid will not spill outside colorimetric cup.
8, SV12 and SV14 valves are responsible for adding cleaning liquid. Before adding
or after the previous adding, open the valve SV12, but SV14 valve is at COM and
NO conduction status (power off status). Because there is a one-way valve in the
3-way top pipeline, cleaning fluid flows into the middle pipeline of the SV12 and
SV14 valves, and time valve SV12, cleaning liquid will remain in pipeline. Open
SV14 valve when adding, cleaning liquid will be added into colorimetric cup through
single way valve under the pressure. About 70ul cleaning liquid is consumed every
time.
47
48
ISE A/D collection
module
ISE ISE valve sets
ISE pump sets
Chapter 6 Instrument Hardware Circuit
module
A/D
Photoelectricity conversion and
(including the control of AC amplification
and pressure temperature
SOPC main control board
R2 adding mechanism
module
R2 reagent disk
R2
PC
monitoring)
R1 adding mechanism
module
R1 reagent disk
R1
Hardware configuration
Sample
module
Sampling mechanism
Sample disk
Reation
module
stirring mechanism
Rinsing mechanism
Reaction disk
6.1
6.2 Security Note:
At working, touching hardware panel with hand or any other objects is forbidden.
In order to dismount panels, operation is only allowed when cut off power (220V, AC).
5、Solenoid valve:SV6、SV11、SV12、SV14、SV15、
SV21、SV30、SV31、SV42、SV43
49
5, buzzer control
7, halogen control
50
2, semiconductor current monitoring and current value
display
51
6.4 Instrument electrical principle wiring
52
Figure 6-4-2 Power switch wiring
53
Figure 6-4-4 Main control board wiring
54
Figure 6-4-5 Solid relay panel wiring
Solid relay
board
12V switch
NES-35-12
55
Figure 6-4-6 Circuit panel control wiring of reagent R1 disk
56
Figure 6-4-7 Circuit panel control wiring of reagent R2 disk
57
Figure 6-4-8 Circuit panel control wiring of reaction disk
58
Figure 6-4-9 Circuit panel control wiring of sample disk
59
Figure 6-4-10 ISE panel control wiring
60
6.5 Circuit panel dismounting
When dismounting, pull out the connector on circuit panel first, and then loosen the set
screws to take out the circuit panel from circuit box.
Analysis part (host) mainly consists of the temperature control system, the reaction
system (including the ISE module), optical detection system, sample and reagent
processing system, mixing system, liquid line system and the reaction cup cleansing
mechanism.
communicate through the motherboard with the reaction disk board, the
sample disk board, reagent 1 disk board , reagent 2 disk board, ISE board, cooling
board, AD board to transmit data and instruction;
61
6.6.3 Reaction disk/ sample disk / reagent 1 disk / reagent 2 disk/ ISE board function
The main function of circuit boards of three disks are to receive the main control
board's instruction to complete the reaction disk, the sample disk, reagent 1 disk, reagent
2 disk, ISE circuit board work, the specific functions as follows:
Each CPU communicates with the main control board to receive instruction;
Main function:
control
cooling system fan
62
6.6.6 Power supply system
NET-50B ±12V
type NET-50B
Output voltage DC 5V,0.6-5A;12V,0.2-2.5A;-12V,0.1-0.7A
Output wattage 50W
Output set 3sets
Temperature -20~+60℃
range
Input voltage 85-264VAC/120-370VDC
size 129*98*38mm
Warranty period 2years
manufacturing Guangzhou
location
NES-15-12 12V
type NES-15-12
Output voltage DC 12V,0-1.3A
Output wattage 15W
Output set 1set
Temperature range -20~+60℃
Input voltage 85-264VAC(120-370VDC)
size 79*51*28mm
Warranty 2 years
period
63
NES-15-5 5V
type NES-15-5
Output voltage DC 5V,0-3A
Output wattage 15W
Output set 1set
Temperature range -20~+60℃
Input voltage 85-264VAC(120-370VDC)
size 79*51*28mm
Warranty 2 years
period
SP-500-24 24V
type SP-500-24
Output voltage DC 24V,0~20A
Output wattage 500W
Output set 1set
Temperature range 0~+40℃
Input voltage 88~264VAC
size 170*120*93mm
Warranty 2 years
period
64
NES-35-12 12V
type NES-35-12
Output voltage DC 12V,0-3A
Output wattage 35W
Output set 1set
Temperature range -20~+60℃
Input voltage 85-264VAC(120-370VDC)
size 99*97*36mm
Warranty 2 years
period
65
Chapter 7 Maintenance and Overhaul
In order to ensure reliable system performance, excellent working status and span,
please conduct system operation and regular maintenance strictly in accordance
with the requirements in the repair manual. Learning maintenance and overhaul of
this chapter is also very important and in-depth study will enable the instrument to
achieve the best running status and exert the best performance.
Warning:
Do not carry out maintenance this chapter doesn’t mention.
Otherwise, it could lead to system damage and personal injury.
Do not touch any other parts except user self-operation and
maintenance which are clear recorded.
Unauthorized repair of the system may lead to system damage
and personal injury, and commitment term of the repair contract is
no longer valid.
Upon completion of maintenance work, make sure the system is
working normally.
Do not splash water, reagent and other liquid onto the system's
mechanical or electrical parts.
Biological contamination danger :
In the process of maintenance work, be sure to wear gloves, put
on work clothes to prevent them from being infected and, if
necessary, wear protective glasses.
7.1Maintenace preparation
Tools, intensified cleaning liquid and alcohol maybe used in the process of working.
7.1.1 Tools
1. One set of hexagon wrench
2. Cruciform Screwdriver (large, medium and small)
3. Injection needle hose
4. Small tweezers
5. Clean gauze
7.1.2 Intensified cleaning liquid
1. Acid cleaning agent, 0.1mol / L hydrochloric acid
2. Alkaline cleaning agent, 0.5% (V / V) sodium hypochlorite
Warning:
Intensified acidic and alkaline cleaning liquid mixed generate
poisonous gas. Do not mix the intensified acidic and intensified
alkaline cleaning liquid.
Caution:
Following cleaning liquid designated by Dirui Co., Ltd.:
Intensified acidic cleaning liquid: 0.1mol / l hydrochloric acid;
Intensified alkaline cleaning liquid: 0.5% (V / V) sodium
hypochlorite.
Please use the intensified cleaning liquid designated by Dirui. If
outside of designated types of intensified cleaning liquid are used, it
may not be able to receive appropriate the results of the analysis.
Dirui recommends the use of alternating acidic and alkaline
cleaning liquid, for example, use intensified acidic cleaning fluid
after power on, then use of intensified alkaline cleaning liquid next
time after power on. 66
7.2 Daily maintenance item
The purpose of checking the injection pump is check whether the leakage exists.
1, Make sure that Power of analysis part has been switched off.
2 open the front door and it is shown as Figure 6-1
If with ISE system, the left are the three ISE unit injection pumps, the middle are
injection pumps of sample, reagent 2, reagent 1 respectively.
Figure 6-1
3. To observe whether the injection pump is leaking,
If so, check the leakage causes, and check the pipeline and connector timely.
2、When cleaning ample and reagent probes, carefully observe whether the
outflow of sample probe internal wall is continuous, whether the direction of flow is
consistent with the sample probe and the outflow of external wall is continuous, ,
and whether water volume is normal.
If still not normal, check the corresponding liquid line channel; check whether
water supply of water tank and water pressure is normal.
67
7.2.4 Rinse rinsing mechanism
1、In online status, click “Instrument resetting” in “Maintenance”, and instrument
executes resetting.
2、When rinsing, carefully observe rinsing probe working and whether probe
infusing is normal and assimilating is completely.
If infusing abnormal, check pressure value of water infusing pressure gauge
If assimilating abnormal, check pressure value of assimilating vacuum
Caution:
Make sure liquid flow catheter was not bent and flows smoothly.
Otherwise, it may result in that poor waste water spills from the cover
panel of analysis part, even the serious damage of analysis part.
68
7.3 weekly maintenance items
Warning:
Please be careful to avoid hands from being scratched
Biological contamination danger:
Do not dispose the gauze used to clean sample probe at your own will,
please follow the relevant provisions for proper disposal.
3 Caution:
When cleaning, do not touch directly the sample
surface to prevent probe scratch; avoid too much hand
force to prevent deformation of the sample probe.
69
Note:
Acidic and alkaline cleaning liquid can be used
alternatively, for instance, acidic cleaning liquid is used
at previous time maintenance, use alkaline cleaning
liquid at this time maintenance.
Wipe the external walls of sample and reagent probes lightly with cotton
stick moisturized with alcohol, especially the point of probe, until no
impurities left at all.
4 Wipe sample and reagent probes with the gauze dipped with deionized
water
5 After cleaning, lift the rotating arms of sample and reagent probes to the top
position, and rotate the rotating arm of sample probe to locate the sample
probe above the rinsing bath of sample and reagent probes.
Caution:
After cleaning the surface of a sample probe, please
make sure sample probe must be rotated to the top of
sample probe rinsing bath.
6 Switch on the power of analysis part and wait 30 seconds, enter the
"maintenance - routine maintenance" column to implement “instrument
resetting", the system will automatically reset the sample and reagent
probes and rinse them with deionized water.
70
7.3.2 Rinse stirring rod
2 Lift the stirring rod by hands to the its top position, and rotate its rotating
arm to the above a position for convenient operation.
3 Caution:
When cleaning, do not touch the sample surface directly to
prevent probe scratch; avoid too much hand force to prevent
deformation of the sample probe.
Note:
Acidic and alkaline cleaning liquid can be used alternatively,
for instance, acidic cleaning liquid is used at previous time
maintenance, use alkaline cleaning liquid at this time
maintenance.
Wipe the surface of stirring rod lightly with cotton stick moisturized with
alcohol, especially the point of probe, until no impurities left at all.
4 Wipe stirring rod with the gauze dipped with deionized water
5 After cleaning, lift the rotating arms stirring rod to the top position, and
rotate the rotating arm of stirring rod to locate the stirring rod to the top of
the rinsing bath of stirring rod.
6 Switch on the power of analysis part and wait 30 seconds, enter the
"maintenance - routine maintenance" column to implement “instrument
resetting", the system will automatically reset the sample and reagent
probes and rinse them with deionized water.
71
7.3.3 Rinse sample/reagent barcode window
小心:
Caution:
Do not gaze scanning laser light, or it may cause eyes injury
Warning:
Please be careful to avoid being scratched by sample probe.
Biological contamination danger:
In operation, please put on gloves, work cloths, and put on protective
glasses for the best.
Do not dispose the gauze used to clean sample probe at your own will,
please follow the relevant provisions for proper disposal.
5 Mount sample disk, tighten disk fixing screws clockwise and cover it.
72
7.3.5 Rinse reagent refrigeration storehouse
Warning:
Please be careful to avoid being scratched by
sample probe.
Biological contamination danger:
In operation, please put on gloves, work cloths, and put on
protective glasses for the best.
Do not dispose the gauze used to clean sample
probe at your own will, please follow the relevant
provisions for proper disposal.
5 Mount sample disk, tighten disk fixing screws clockwise and cover it.
73
7.4 Monthly maintenance item
Warning:
Please be careful to avoid being scratched by
sample probe.
Biological contamination danger:
In operation, please put on gloves, work cloths, and put on
protective glasses for the best.
Do not dispose the gauze used to clean sample
probe at your own will, please follow the relevant
provisions for proper disposal.
2 Lift the rotating arm of sample probe by hands to the its top position, and
rotate its rotating arm to keep sample probe away from rinsing bath for
convenient operation.
3 Clean the inside and appearance of sample probe rinsing bath with clean
cotton stick.
4 After cleaning, lift the rotating arm of sample probe to the top position, and
rotate the rotating arm of stirring rod to locate the sample probe to the top
of the rinsing bath of sample probe.
Caution:
After the work of sample probe surface rinsing, please
make sure to rotate the sample probe to the top of
sample probe rinsing bath.
5 Switch on the power of analysis part and wait 30 seconds, enter the
"maintenance - routine maintenance" column to implement “instrument
resetting", the system will automatically reset the sample and reagent
probes and rinse them with deionized water.
74
7.4.2 Rinse stirring rod rinsing bath
Warning:
Please be careful to avoid being scratched by
sample probe.
Biological contamination danger:
In operation, please put on gloves, work cloths, and put on
protective glasses for the best.
Do not dispose the gauze used to clean sample
probe at your own will, please follow the relevant
provisions for proper disposal.
75
7.6 Every half a year maintenance item
Lamp light source of optical system will gradually be aged in use, and will cause an
increase in noise measurements. If the cup blank and light source intensity
attenuation is out of range or the working time of light source lamp accumulates over
2000 hours, the light source lamp should be checked.
Caution:
Please use consumables recommended by Dirui company, using other
consumables may cause system performance degradation.
Do not touch the light source lamp shell surface and lens in front of the
light source lamp by hand, because it may change the characteristics
of the light source. If you accidentally make noodle stained with filth,
absorbent cotton dipped by absolute alcohol can be used to clean it.
1 Turn off the system main power, so that the light source box and light
source lamp will be cooled for at least 15 minutes.
Warning:
High-temperature light source lamp and light box will
cause burn. Operation is carried out only after the light
source and light source lamp are cooled.
76
3 Loosen the two screws fixing light source seat to remove halogen lamp.
4 Mount a new halogen lamp according to the above opposite steps; pay
attention to tighten the screws. The cooling rubber hose in the lamp room
can not be twisted and down-lead can not be loosed or cocked.
5 Remount the reaction disk, the reaction cup and rinsing mechanism;
switch on the power supply of analysis part. After standby mode,
single-click “Next” in “System maintenance” window; infuse purified water
into reaction groove. After instrument standby mode, execute light quantity
check function. Check the back of halogen lamp if the light quantity
conforms to the requirement to start test.
77
7.8 Irregular check item
If the water flow is not normal when cleaning sample probe, sample probe and reagent
probe may have been blocked and cleaning is needed to sample probe.
Warning:
Please be careful to avoid being scratched by
sample probe.
Biological contamination danger:
In operation, please put on gloves, work cloths, and put on
protective glasses for the best.
Do not dispose the gauze used to clean sample
probe at your own will, please follow the relevant
provisions for proper disposal.
78
warning:
Carefully place dismounted sample probe and prevent it
scratching human body and sample probe damage.
Note:
Take out sample probe from the rotating arm and be careful
to operate to avoid the damage of probe point caused by
touching rotating arm.
Note:
Sample probe is precisely processed to ensure the sample adding
precision. If the probe point is damaged or bent, checking sample
probe is a must, or no guarantee can be made for test precision. Please
refer to "Error! Reference source not found. Error! Reference source
not found." for specific check of sample probe.
Warning:
Please be careful to avoid being scratched by sample probe.
Biological contamination danger:
In operation, please put on gloves, work cloths, and put on protective
glasses for the best.
Do not dispose the gauze used to clean sample probe at
your own will, please follow the relevant provisions for
proper disposal.
Caution:
Sample probe is precisely processed to ensure the sample adding
precision. If the probe point is damaged or bent, checking sample
probe is a must, or no guarantee can be made for test precision.
Please refer to "Error! Reference source not found. Error! Reference
source not found." for specific check of sample probe.
79
7.8.3 Install sample/reagent probe
Warning:
Please be careful to avoid being scratched by sample probe.
Caution:
Sample probe is precisely processed to ensure the sample adding
precision. If the probe point is damaged or bent, checking sample
probe is a must, or no guarantee can be made for test precision.
Please refer to "Error! Reference source not found. Error! Reference
source not found." for specific check of sample probe.
When it is found that the water level of rinsing bath is too high when rinsing sample
probe because of no discharging available, which may be caused by the blocked
leaking hole. Cleaning sample rinsing bath is necessary.
Warning:
Please be careful to avoid being scratched by sample probe.
2 Lift the rotating arm of sample probe by hands to its top position, and rotate
its rotating arm to keep sample probe away from rinsing bath for
convenient operation.
3 Infuse about 1ml alkaline detergent of 0.5% (V / V) sodium hypochlorite or
84 disinfectant into rinsing bath for 10 minutes.
4 Switch on the power supply of analysis part
5 Lift the rotating arm of sample probe by hands to its top position, and rotate
its rotating arm to keep sample probe above the rinsing bath of sample
probe.
Caution:
Please rotate the sample probe to the top of sample probe
rinsing bath after clean rinsing bath of sample probe.
80
7.8.5 Rinse stirring rod
If the stirring rod is damaged, please check stirring rod in accordance with following
steps strictly.
Warning:
Please be careful to avoid being scratched by sample probe.
Any touch is forbidden except at the knurling of it only by hand when
checking, and prevent any scratch on the flat part of stirring part.
Biological contamination danger:
In operation, please put on gloves, work cloths, and put on protective
glasses for the best
Please deal with removed stirring rod properly.
Caution:
Please use consumables recommended by Dirui company, using other
consumables may cause system performance degradation.
2 Prepare a new stirring rod and wipe the flat part of it with gauze or cotton
stick dipped with cleaning liquid, and then wipe it with gauze dipped with
deionized water.
3 Lift the rotating arm of stirring rod by hands to its top position, and rotate its
rotating arm for convenient operation.
81
Caution:
When pulling out stirring rod, make sure the direction of
force is vertical to that of axis of rotating arm. Lateral force
may damage the axis or stirring rod.
5 Prepare a new stirring rod, and wipe the front of the stirring rod with gauze
dipped with 2% CS-antibacterial cleaning liquid.
6 When mounting new stirring rod, insert stirring rod till the bottom of motor
axis and tighten it with M2 screw.
Caution:
When inserting stirring rod, make sure the direction of force is
vertical to that of axis of rotating arm. Lateral force may
damage the axis or stirring rod.
Pushing the stirring rod completely
7 After stirring rod check, visually check whether stirring rod and its rotating
arm are vertical with each other.
If not vertical, return to step 5 and remount the stirring rod.
If vertical, continue to next.
8 Lift the rotating arm of stirring rod by hands to its top position, and rotate its
rotating arm to the top of its rinsing bath.
Caution:
Please make sure to rotate stirring rod to its rinsing bath top
after mount it.
9 Switch on the power of analysis part and wait 30 seconds, enter the
"maintenance - routine maintenance" column to implement “instrument
resetting", the system will automatically reset the sample probe and rinse it
with deionized water. Observe the outflow of sample probe.
82
7.8.6 Check reaction cup
Warning:
Please be careful to avoid being scratched by sample probe.
Place each probe and pole into proper position for convenient.
Biological contamination danger:
In operation, please put on gloves, work cloths, and put on protective
glasses for the best
Please deal with removed reaction cup properly which is broken.
Caution:
Please use consumables recommended by Dirui company, using other
consumables may cause system performance degradation.
83
3 Rotate reaction disk by hand and remove the reaction cup sequently. Take out
reaction cup while rotating it.
4 Rinse the new reaction cup dipped in with water; rinse inside and outside of
reaction and no scratch is allowed.
5 Rotate reaction disk by hands, and mount new reaction cup on reaction disk
and check the six sets reaction cups simultaneously.
6 Mount reaction disk with the opposite steps and make sure the fixing screw of
reaction disk is tight.
7 Switch on the power of analysis part
Select and execute “cup blank test” after click “System maintenance”, and
observe execution result and reaction status.
84
Chapter 8 Assay Method
point assay
two-point assay
rate assay
The assay mode of Auto-Chemistry Analyzer is based on the Beer-Lambert law that the
material selective absorption light
The main principle is: When monochromatic light with specific wavelength passes
through the cuvette with sample, the monochromatic light absorbency and sample liquid
concentration are varies directly as the distance which is passed through sample liquid by
light:
I0
A = lg (1/T )= lg ( )= ε b c
It
A -Absorbency of the light when passing through liquid
I0 - Incident intensity
It - Transmitted intensity
Solution layer thickness (b): Optical path, which is fixed by instrument. Molar absorption
coefficient (ε) is the correlation coefficient of the wavelength, solution and solution
temperature. Linear relationship is displayed between solution thickness and absorbency
when in stable temperature and single wavelength(ε value is given on the reagent bottle by
factory)
If the sample liquid adequate distribution, interaction between liquid and incidence
monochromatic light only happens during absorbing process. No fluorescence, disperse and
photochemical appear. No interaction between substances in the solution while absorbing
process. The absorbency possess conducts nature, and this condition conforms to the
Beer-Lambert law
85
8.2 Assay mode variety
Cell
Method Photometry point Formula Remark
blank
L– 0– 0– 0 B1 + B 2 + B 3 AL + AL −1
1-point
Assay 1<L≤49 3 2
L– M– 0- 0 B1 + B 2 + B 3 ( AM + AM −1 ) − k ( AL + AL −1 )
2-point
assay 3 2
1<L<M≤49
AM + AM −1 AL + AL −1 测光点 L、
2-point B1 + B 2 + B 3 −
2 2 M 间的时
Rate Assay L– M– 0– 0 3
t 间(分)
1<L<M≤49
L– M – 0 - 0
Rate A B1 + B 2 + B 3
1<L<M≤49 △A(M-L)
Assay 3
L +2<M
Fist half
1-point & L– 0 – 0 – 0 B1 + B 2 + B 3 AL + AL −1
Rate Assay 3 2
1 < M < N≤L < P <
Q≤49
86
Second half
M–N–P–Q
B1 + B 2 + B 3
1 < M < N≤L < P < △(AQ- P)-k△(AN -M)
3
Q≤49
M+2<N ,P+2<Q
Fist half
B1 + B 2 + B 3 AL + AL −1
L– 0 – 0 – 0
3 2
1<L≤M<N≤49
3-point dual
item
Second half
( AN + AN −1 ) − k ( AM + AM −1 )
B1 + B 2 + B 3
M–N–0–0 2
3
1<L≤M<N≤49
Fist half
L– M – 0 - 0 B1 + B 2 + B 3
△A(M-L)
3≤L<M<N<P≤49 3
L +2<M
Rate B
Assay △ An 、 p ( diffe
(mode 1 ) Second half wavelength
N–P–0–0 B1 + B 2 + B 3 from the first Half)
Two conditions
3≤L<M<N<P≤49 3
△An、p–k△AL、m(same
N+2<P wavelength as the second
half)
Fist half
L– M – 0 – 0
Rate B B1 + B 2 + B 3
Assay 3≤L<M<N<P<Q △A(M-L)
(mode 2 ) 3
<R≤49
L +2<M
Second half
N – P –Q – R
B1 + B 2 + B 3
3≤L<M<N<P<Q △A(R-Q)–k△A(P-N)
3
<R≤49
N+2<P ,Q+2<R
87
L,m,n,p,q,r : Photometric points
S :Sample volume
Note 1: The 5 th Photometric point won’t be Stirred after adding reagent 2. Stirring
when the reaction disk pauses after rotates one circle plus 2 pitches
Note 2: liquid in the reaction cuvette should be more than or equal to 150 ul, less
than or equal to 450ul.
88
8.3 Endpoint assay
Endpoint assay means the reaction takes a period time. Due to reaction balance constants
are big, all substrates (tested) are transformed into product when reaction reaches balance,
and no increase (decrease) of reaction solution absorbance will occur, and the degree of
absorbance increase (decrease) and the concentration of tested substance is directly
proportional. This assay is call “endpoint assay” or balance assay more accurately, which is
the ideal assay mode.
The endpoint assay is not sensitive to small changes (such as enzyme amount, pH,
temperature, etc.) as long as this change does not affect the balance in certain time.
Cell blank
Time
89
Example 1:TBIL-Total bilirubin reagent kit
90
8.4 2-point assay
2-point assay (fixed time assay) is also called first class dynamics assay, which means the
reaction speed is in direct proportion to the simple power of substrate concentration in
specified time, namely v=k[S]. Due to the reduction of substrate, the whole reaction speed
is decreasing gradually, which reflects the decrease (increase) of absorbance and
decrease of speed. Because reaction time to reach balance is very long, , it can be
monitored at any time theoretically, but because of the complexity of serum ingredient and
much reaction, therefore, it takes a certain period of time to enter in stable reaction phase.
Absorbance
Reaction limit level
Absorbance
Cell blank
Time
Time
2-point assay 2-point rate assay
Rate assay, also known as zero-class dynamics assay, refers to the reaction rate is directly
proportional to the zero power of substrate concentration, which has nothing to do with the
substrate concentration. Hence, the reactants can generate a certain product at constant
speed throughout the reaction process, resulting in even decrease or increase of
absorbance of measured solution at a wavelength, and the decrease or increase speed (△
A / min) is directly proportional to the activity or concentration of the tested substance
(catalytic material). Dynamics assay is also called as the continuous monitoring assay,
mainly used for the measurement of enzyme activity.
In fact, because substrate concentration can not be big enough, with the reaction
proceeding, the reaction is no longer zero class when substrate is consumed to a certain
extent, Therefore, zero-class dynamics assay is targeted at a specific period in terms of
time; Because reaction time to reach balance is very long, , it can be monitored at any time
theoretically, but because of the complexity of serum ingredient and much reaction,
therefore, it takes a certain period of time to enter in stable reaction phase. So all reagent
manufactures have strict requirements to the two periods
Dynamics assay is based on the changes between specified photometric points to obtain
the absorbance concentration or activity value.
Metering point in accordance with the input form, dynamics method can be divided into
single-band and dual-band dynamics assay.
91
Figure 8-3 Rate assay reaction curve
Absorbance
Time
Example 3:ALT/GPT - Alanine aminotransferase (IFCC)
Calculating method:
A/min * TV *1000
ALT(U/L)=
6.22 * SV * P
TV=The total reaction volume (mL)
SV=sample volume(mL)
92
8.6 Principle of electrolyte measurement
Principle
Internal standard solution is firstly added in diluting trough by instrument, and assimilates it
and discharges it into the Na, K, and Cl electrode solution line through the SIP injection
pump to measure its electrode potential which is relative to reference electrode potential.
At this time, SIP injection pump first assimilates reference electrode solution and
discharges it into the reference electrode solution line, and then switch to liquid line to
assimilate the internal standard. And redundant internal solution is assimilated by vacuum
nozzle to vacate diluting trough.
Diluent will be mixed after the sample probe assimilated sample and discharged it into
diluting trough. Diluted sample is as same as the internal standard solution and will be
taken out by SIP syringe pump and the internal standard solution is add into diluting trough
to rinse it. Used for cleaning, the internal standard solution will be pumped through the SIP
pump. After vacate diluting trough, assimilate the internal standard solution for the next
round of testing.
93
94
Assimilate internal solution to
rinse diluent bath
Measure sample concentration
Assimilate diluted sample from
diluent bath
Diluting sample
Infuse sample Discharge internal standard
solution
Measure and calibrate the concentration of
internal standard solution
Figure 8-4 ISE Work flow
RT
E = E 0 + 2.303 × × l og(ai )
nF ………………………………………………………(1)
ai = f × Ci ……………………………………………………………………………(2)
f: activity coefficient
Ci: concentration
n: a given ion (i) the charge number (Cation is positive, anion is negative)
Measure low concentration slope of liquid (S1) and high concentration slope liquid (S2),
and determine slope value (sensitivity) of K, Na, Cl the electrode.
E ( H ) − E ( L)
SL =
C(H )
log
C ( L) …………………………………………………… (3)
95
8.6.3.2 The measurement of internal liquid concentration
E ( IS ) − E ( L )
C ( IS ) = C ( L) × 10 SL
…………………………………………… (4)
The calculation of routine sample, STAT sample, and concentration of quality control liquid
is based on the concentration of internal standard solution. Internal standard solution is
different with the different sample.
E ( S ) − E ( IS )
C ( S ) = C ( IS ) × 10 SL
………………………………………… (5)
C(S):Sample concentration
Test calibrator (calibrator S3)of serum category after calibration to calculate its
concentration, and the difference between the tested concentration and input value is used
as compensation value to increase or decrease sample quantitative value.
C ' ( S ) = IF {C ( S ) + C (VALUE )}
.. ... ... ... ... ... ... ... ... ... ... ... ... ... (7)
96
8.6.3.5 Standard specification of electrolyte
Item specification
Sample 15ul
volume
Diluent volume 450ul
Processing 80sample/h(only measuring electrolyte)
ability
Measuring Na + 20 ~ 200mmol / L (when only serum )
range 10 ~ 400mmol / L (when measuring urine)
K + 1.0 ~ 15.0mmol / L (when only serum)
1 ~ 200mmol / L (when measuring urine)
Cl-20 ~ 200mmol / L (when only serum)
10 ~ 400mmol / L (when measuring urine)
Note:
Internal standard solution will be added if there is no electrolyte analysis for more than 10
minutes in order to activate the electrode.
97
Chapter 9 Malfunction Analysis
Alarm Detailed
Description Solution
Code description
Solution:
98
(1) Check whether the conductivity from adapter to
reaction disk circuit board is good and both ends of
connector are connected well.
(1)check lead
solution:Mechanical repair
R1stirring
mechanism
R1 Stirring fails to
1-2 mechanism reach the ¾ R1stirring mechanism fails to reach the top,the
abnormal top of solution is the same as 1-1
reaction
side.
99
mechanical repair is required if resistance is big.
Solution:
Solution:
100
of right limit light sensor.
1)check lead
1)check lead
101
¾ In the CS-400 upper instrument software, enter
into the "system maintenance" interface after
on-line, implement "mechanical movement
check", and observe the stirring rod running
status
Malfunction 1:stirring mechanism stops
Solution:
1)check lead
102
2)check lead
R1 Stirring When
1-6 mechanism resetting, The solution is the same as 1-4
abnormal R1 Stirring
mechanism
failed to
reach
rinsing
bath.
R1 Stirring
mechanism
R1 Stirring
failed to ¾ If stirring mechanism failed to reach the top when
1-8 mechanism
reach the rotating,the solution is the same as 1-1
abnormal
top when
rotating.
103
9.2 Stirring mechanism
Solution:
(1)check lead
104
(2)check light sensor signal
solution:Mechanical repair
R2 Stirring
R2 Stirring
mechanism can
mechanis ¾ R2 stirring mechanism fails to reach the top,the
2-2 not reach the top
m
at reaction cup solution is the same as 2-1
abnormal
side.
105
¾ In the CS-400 upper instrument software, enter
into the "system maintenance" interface after
on-line, implement "mechanical movement
check", and observe the stirring rod running
status
Malfunction 1::stirring mechanism stops
Solution:
1)check lead
1)check lead
106
to light sensor lead(P272-P402)pin 4、5、6 is good
and both ends of connector are connected well.
Solution:
Solution:
107
2、in boot-strap status,,manually sway stirring
mechanism,observe the left_ st2indicator status of
right limit light sensor.
(1)sparkling of left_ st2 indicator means the light
sensor, lead and adaptor are normal.
1)check lead
Check whether the conductivity from adapter J285 to
reaction disk circuit board lead (P285-P075)is
good and both ends of connector are good.
2)Check input part circuit of reaction disk circuit
board light sensor signal.
(2)if no sparkling of left_ st2,check left_ st2
1)Check whether the ud_ st2 indicator has
malfunction
2)check lead
Check whether the conductivity from adapter
J273 to light sensor lead (P285-P075)pin 1、
2、3 is good and both ends of connector are
connected well.
3)check light sensor signal
check voltage between P272 plug pin 1,3, if
the voltage is not 5v, see(3); if voltage is 5v,
check the potential of socket pin 2 of the P272.
The potential is low when sway to reaction cup,
otherwise the potential is high. If the potential
is normal, indicator remains, check the adapter
board; not normal, check the light sensor.
4 )check voltage between P285 plug pin 4,6,
if the voltage is not 5v, check adapter board; if
voltage is 5v, check conductivity from adapter
board J285 to reaction disk lead(P285-P075)
is good and both ends of connector are
connected well.
R2 Stirring
R2 Stirring
mechanism can
mechanis
2-6 not reach The solution is the same as 2-4
m
rinsing bath
abnormal
when resetting.
R2 Stirring
R2 Stirring
mechanis ¾ If stirring mechanism failed to reach the top
2-8 mechanism fails
m when rotating,the solution is the same as 2-1
to rotate at top.
abnormal
108
9.3 Rinsing mechanism of reaction cup
Solution:
1)check lead
109
normal, indicator remains, check the adapter
board; not normal, check the light sensor.
solution:Mechanical repair
Solution:
110
9.4 Reaction disk
Liquid level of
cleaning
alkaline
4-6 liquid level Add cleaning liquid into cleaning liquid kit.
cleaning liquid
low
kit low.
111
9.5 Sample probe
Alarm Detailed
Description Solution
Code description
Solution:
(1)check lead
(1)check lead
112
ends of connector are good.
solution:Mechanical repair
Solution:
113
1、sparkling of ud_s indicator means the wiring of
light sensor , lead and adaptor is normal.
(1)check lead
(1)check lead
Sample
probe fails to
Sample probe leave the top
5-6 The solution is as same as 5-5
abnormal. (reaction cup
side) when it
descending.
114
surface_s put out.
(1)check lead
115
J7-level status from right to left (J7: 1 empty,
2touch, 3surface) (J6: 1 earth, 2 empty, 3 +5 v,
4 blank)
Solution:
(1)check lead
116
(2) check sample circuit board
(1)check lead
solution:Mechanical repair
as 5-8
117
Sample
probe
Sample probe ¾ Sample probe fails to reach the top and the
5-12 deviates from
abnormal. solution is as 5-1
top when it
rotates.
solution:
Alarm Detailed
Description Solution
Code description
solution:
Malfunction 2:
When sample disk rotates to the designated position,
118
sample disk fails to stop at the designated position or
the light sensor detects it after it reaches the designated
position.
solution:
Manually rotate reagent disk and observe adapter cnt_s
indicator status.
(1)sparkling of cnt_s indicator means the light sensor,
lead and adaptor are normal.
1)check lead
Check whether the conductivity from adapter J285 to
reaction disk circuit board lead is good and both ends
of connector are good.
2)Check input part circuit of reagent disk circuit board
light sensor signal.
2、if no sparkling of cnt_s, check cnt_s
1)check lead
Check whether the conductivity from adapter J278 to
light sensor lead (P277-P408)is good and both ends
of connector are connected well.
2)check light sensor signal
check voltage between P278 plug pin 7,9, if the voltage
is not 5v, see(3); if voltage is 5v, check the potential of
socket pin 8 of the P278. The potential is high when
rotating to the zero position, otherwise the potential is
low. If the potential is normal, check the adapter board;
not normal, check the light sensor
3)check voltage between P282 plug pin 4,6, if the
voltage is not 5v, check adapter board; if voltage is 5v,
check conductivity from adapter board J282 to reaction
disk lead(P282-P048)is good and both ends of
connector are connected well. If all are right, check
sample disk circuit board.
Malfunction 1:sample disk stops
Sample disk solution:
Sample disk fails to find 1, rotate reagent disk, and mechanical repair is required
6-7 if resistance is big.
abnormal the zero
position. 2, check whether both ends of connector of the motor
are connected well.
3, check whether the conductivity of motor lead wire is
good.
4, check the motor driving unit of sample disk circuit
board is working normally
119
Malfunction 2:
When sample disk rotates to the designated position,
sample disk fails to stop at the designated position or
the light sensor detects it after it reaches the designated
position.
solution:
Manually rotate reagent disk and observe adapter
zero_sdisk indicator status.
(1)sparkling of zero_sdisk indicator means the light
sensor, lead and adaptor are normal.
1)check lead
Check whether the conductivity from adapter J282 to
reaction disk circuit board lead is good and both ends of
connector are good.
2)Check input part circuit of reagent disk circuit board
light sensor signal.
2、if no sparkling of zero_sdisk, check zero_sdisk
1)check lead
Check whether the conductivity from adapter J278 to
light sensor lead (P277-P408)is good and both ends
of connector are connected well.
2)check light sensor signal
check voltage between P278 plug pin 1,3, if the voltage
is not 5v, see(3); if voltage is 5v, check the potential of
socket pin 2 of the P278. The potential is high when
rotating to the zero position, otherwise the potential is
low. If the potential is normal, check the adapter board;
not normal, check the light sensor
3)check voltage between P282 plug pin 4,6, if the
voltage is not 5v, check adapter board; if voltage is
5v, check conductivity from adapter board J282 to
reaction disk lead(P282-P048)is good and both
ends of connector are connected well. If all are
right, check sample disk circuit board
Solution:
120
9.7 Sample injection pump
Alarm Detailed
Description Solution
Code description
solution:
121
circuit of sample disk circuit board
122
9.8 R1 reagent probe
Alarm Detailed
Description Solution
Code description
solution:
1)check lead
123
2)check light sensor signal
solution:
124
motor are connected well.
1)check lead
125
Malfunction: stays the valid status of collision and
liquid level detection
(1)check lead
126
(surface),9(touch)is normal.
127
but light sensor can not detect the signal
(1)check lead
solution:mechanical repair
128
rotates from solution:As 8-5
reaction cup
Malfunction 2:rotating arm can leave reaction
cup, but light sensor can not detect the signal
solution:As 8-5
R1 reagent
R1 reagent probe
8-7 probe deflects ¾ Probe fails to reach the top and solution is the
abnormal when same as 8-1
rotating
solution:
Liquid level is
R1 reagent not 1、check whether the wiring conductivity of liquid level
8-8 probe detected at detection board and reagent circuit board is good,
abnormal R1 reagent and both ends of connector is connected well.
position
2、check the circuit board of liquid level detection and
test whether the sensitivity of reagent probe is
normal
solution
129
9.9 R2 reagent probe
Alarm Detailed
Description Solution
Code description
solution:
1)check lead
130
2)check light sensor signal
solution:
131
motor are connected well.
1)check lead
132
Malfunction: stays the valid status of collision and
liquid level detection
(1)check lead
133
(surface),9(touch)is normal.
134
but light sensor can not detect the signal
(1)check lead
solution:mechanical repair
R2 reagent
R21 reagent ¾ Enter into the "system maintenance" interface
probe fails to
9-6 probe after on-line, implement "mechanical movement
leave
abnormal check", and observe the reagent probe running
reaction cup
status
when it
135
rotates from Malfunction 1:reagent probe can not sway
reaction cup
solution:As 8-5
solution:As 8-5
R2 reagent
R2 reagent
probe ¾ Probe fails to reach the top and solution is the
9-7 probe
deflects same as 8-1
abnormal
when rotating
solution:
Liquid level is
R2 reagent not detected 1、check whether the wiring conductivity of liquid level
9-8 probe at R2 detection board and reagent circuit board is good,
abnormal reagent and both ends of connector is connected well.
position
2、check the circuit board of liquid level detection and
test whether the sensitivity of reagent probe is
normal
solution
136
9.10 R1 reagent disk
Alarm Detailed
Description Solution
Code description
10-1 No No No
solution:
1)check lead
1)check lead
137
Check whether the conductivity from adapter J275
to light sensor lead is good and both ends of
connector are connected well.
solution:
Malfunction 2:
solution:
138
1)check lead
Check whether the conductivity from adapter J283 to
reagent 1 circuit board lead is good and both ends of
connector are good.
2)Check it circuit board of reagent 1
2、if no sparkling of zero_r1_disk, check zero_r1_disk
1)check lead
Check whether the conductivity from adapter J275
to light sensor lead (P275-P405) is good and
both ends of connector are connected well.
2)check light sensor signal
check voltage between P275 plug pin 1,3, if the
voltage is not 5v, see(3); if voltage is 5v, check the
potential of socket pin 2 of the P275. The potential
is high when rotating to the zero position, otherwise
the potential is low. If the potential is normal, check
the adapter board; not normal, check the light sensor
3)check voltage between P283 plug pin 4,6, if
the voltage is not 5v, check adapter board; if
voltage is 5v, check conductivity from adapter
board P283 to reagent 1 lead(P282-P048)is
good and both ends of connector are connected
well. If all are right, check reagent 1 circuit
board
139
9.11 R2 reagent disk
Alarm Detailed
Description Solution
Code description
solution:
1)check lead
140
1)check lead
solution:
Malfunction 2:
solution:
141
zero_R2_disk indicator status.
(1)sparkling zero_R2_disk indicator means the light
sensor, lead and adaptor are normal.
1)check lead
Check whether the conductivity from adapter J284 to
reagent 2 circuit board lead is good and both ends of
connector are good.
2)Check it circuit board of reagent 2
2、if no sparkling of zero_R2_disk, check
zero_R2_disk
1)check lead
Check whether the conductivity from adapter J277 to
light sensor lead is good and both ends of connector
are connected well.
2)check light sensor signal
check voltage between P275 plug pin 1,3, if the
voltage is not 5v, see(3); if voltage is 5v, check the
potential of socket pin 2 of the P277. The potential
is high when rotating to the zero position, otherwise
the potential is low. If the potential is normal, check
the adapter board; not normal, check the light sensor
3)check voltage between P284 plug pin 4,6, if the
voltage is not 5v, check adapter board; if voltage is
5v, check conductivity from adapter board P284 to
reagent 2 lead is good and both ends of connector
are connected well. If all are right, check reagent 2
circuit board
142
9.12 R1 injection pump
Alarm Detailed
Description Solution
Code description
143
Malfunction:R1injection pump stops or fails to leave
the top
solution:
144
9.13 R2 injection pump
Alarm Detailed
Description Solution
Code description
solution:
145
Malfunction:R2 injection pump stops or fails to
leave the top
solution:
146
9.14 Reaction bath
Alarm Detailed
Description Solution
Code description
147
9.15 ISE
ISE is over
ISE is measuring
over lower limit, and (1)Please check whether the sample
0-31 Note measuring volume, reagent volume is adequate,
lower there is no and whether the position is correct
limit result of
current QC
ISE is over
ISE is measuring
over lower limit, and (1)Please check whether the sample
0-32 Note measuring volume, reagent volume is adequate,
lower there is no and whether the position is correct
limit result of
current QC
ISE calibration
ISE is is over
over measuring (1)Please check whether the sample
0-33 Note measuring lower limit, and volume, reagent volume is adequate,
lower and whether the position is correct
limit there is no
result of
current QC
ISE calibration
ISE is is over
over measuring (1)Please check whether the sample
0-34 Note measuring lower limit, and volume, reagent volume is adequate,
lower and whether the position is correct
limit there is no
result of
current QC
ISE sample
ISE is is over
over measuring (1)Please check whether the sample
0-35 Note measuring lower limit, and volume, reagent volume is adequate,
lower and whether the position is correct
limit。 there is no
result of
current QC
148
Electrolyte
system stops
working due to
Electrolyte the issued (1)Enter into system maintenance,
alarm. Note:
function and execute "Resetting”, and then
19-1 Note the status of
execute “Mechanical movement
stops adding sample
check”
means to
restart.
remaining
volume of
(1)add ISE reference liquid
ISE reference
referenc e liquid is (2)Enter into system maintenance,
37-1 Note inadequate inadequate and execute "ISE reference liquid
(less then the
reagent pipeline rinsing”
volume user
designed) (3)Execute ISE calibration
remaining
volume of
ISE (1)add ISE internal standard liquid
internal
internal standard liquid (2)Enter into system maintenance,
standard is inadequate
38-1 Note and execute "ISE internal standard
liquid is (less then the
liquid reagent pipeline rinsing”
inadequate volume user
designed) (3)Execute ISE calibration
remaining
volume of (1)add ISE diluent
diluent
ISE (2)Enter into system maintenance,
liquid is
39-1 Note diluent is and execute "ISE diluent reagent
inadequate
inadequate (less then the pipeline rinsing”
volume user (3)Execute ISE calibration
designed)
149
average value
(EAV)of three
potentials is
over the
following
range
(internal
standard
solution)Na:
-90.0mv≤EAV
≤-10mv
In the
5-measuring
point
potential of
internal
standard
solution, the (1) Enter into system maintenance,
average value and execute "ISE check."
ISE
60-2 Note LEVEL (EAV)of three
error potentials is (2) please refer to " electrolyte device
over the maintenance " in the "user manual" for
following the detailed
range
(internal
standard
solution)K:
-90.0mv≤EAV
≤-10mv
In the
5-measuring
point
potential of
internal (1) Enter into system maintenance,
standard and execute "ISE check."
ISE solution, the
60-3 Note LEVEL average value
error (2) please refer to " electrolyte device
(EAV)of three maintenance " in the "user manual" for
potentials is the detailed
over the
following
range
(internal
standard
150
solution)Cl:
80.0mv≤EAV≤
160mv
In the
5-measuring
point
potential of
internal
standard (1) Enter into system maintenance,
solution, the and execute "ISE check."
ISE difference(FI)
61-1 Note Noise of the
error maximum and (2) please refer to " electrolyte device
minimum is maintenance " in the "user manual" for
within the detailed
following
range
Na:
0.7mv≤|FIV(2)
-FIV(4)|
In the
5-measuring
point
potential of
internal
standard
solution, the (1) Enter into system maintenance,
difference and execute "ISE check."
ISE (FIV) of the
61-2 Note Noise maximum and
error minimum is (2) please refer to " electrolyte device
within maintenance " in the "user manual" for
following the detailed
range (internal
standard,
sample)
K:
1.0mv≤|FIV(2)
-FIV(4)|
In the
(1) Enter into system maintenance,
5-measuring
ISE and execute "ISE check."
point
61-3 Note Noise
error potential of (2) please refer to " electrolyte device
internal maintenance " in the "user manual" for
standard the detailed
151
solution, the
difference
(FIV) of the
maximum and
minimum is
within
following
range (internal
standard,
sample)
Cl:0.8mv≤|FIV
(2)-FIV(4)|
The slope
value of
calibration
result is within
following
range or the
impact of
(1) Enter into system maintenance,
ISE electrode is
and execute "ISE check."
Preparation low
62-1 Note abnorma l (cross-contami
nation rate (A) (2) please refer to " electrolyte device
is as following maintenance " in the "user manual" for
the detailed
Na:(1)
32.0mv≤SLOPE
≤37mv or
68.1mv≤SLOPE
The slope
value of
calibration
result is within (1) Enter into system maintenance,
ISE following and execute "ISE check."
Preparation range or the
62-2 Note abnorma l impact of
electrode is low (2) please refer to " electrolyte device
(cross-contami maintenance " in the "user manual" for
nation rate (A) the detailed
is as following
K:(1)
32.0mv≤SLOPE
≤37mv
or68.1mv≤SLOPE
152
The slope
value of
calibration
result is within
following (1) Enter into system maintenance,
range or the and execute "ISE check."
ISE impact of
electrode is
Preparation (2) please refer to " electrolyte device
low
62-3 Note abnorma l
(cross-contami maintenance " in the "user manual" for
nation rate (A) the detailed
is as following
Cl:(1)
-30mv≤SLOPE
≤-25mv
or-68.1mv≥SL
OPE
(1)The slope
value of
calibration
result is within
following
range or the (1) Enter into system maintenance,
impact of and execute "ISE check."
electrode is
low
ISE (cross-contami (2) please refer to " electrolyte device
SLOPE nation rate (A) maintenance " in the "user manual" for
63-1 Note
abnorma l is as following: the detailed
Na:
(1)SLOPE
<32.0mv (3) Please re-execute ISE calibration.
(2)current IS
calibration
result is not
updated
153
impact of
electrode is
low
(cross-contami (3) Please re-execute ISE calibration.
nation rate (A)
is as
following:K:
(1)SLOPE<
32mv
(2)current IS
calibration
result is not
updated
(1)The slope
value of
calibration
result is within
following (1) Enter into system maintenance,
range or the and execute "ISE check."
impact of
electrode is
ISE low (2) please refer to " electrolyte device
SLOPE (cross-contami maintenance " in the "user manual" for
63-3 Note
abnormal nation rate (A) the detailed
is as
following:Cl:
(1)SLOPE> (3) Please re-execute ISE calibration.
-25.0mv
(2)current IS
calibration
result is not
updated
(1)the
concentration
of internal (1) Enter into system maintenance,
liquid(C(IS)) and execute "ISE check."
ISE the is within
concentr following
ation of (2) please refer to " electrolyte device
range :Na:C maintenance " in the "user manual" for
64-1 Note internal
(IS)< the detailed
liquid
abnorma l 120.0mmol/l or
190.0mmol/<
C(IS)。 (3) Please re-execute ISE calibration.
(2)current IS
calibration
result is not
updated
154
(1)the
concentration
of internal
liquid(C(IS)) (1) Enter into system maintenance,
is within and execute "ISE check."
following
ISE the
concentr range .K:C
ation of (IS)< (2) please refer to " electrolyte device
64-2 Note internal 3.0mmol/l or maintenance " in the "user manual" for
liquid 8.0mmol/ <C the detailed
abnorma l (IS)。
(3) Please re-execute ISE calibration.
(2)current IS
calibration
result is not
updated
(1)the
concentration
of internal
(1) Enter into system maintenance,
liquid(C(IS))
and execute "ISE check."
is within
ISE the following
concentr range Cl:C
ation of (2) please refer to " electrolyte device
(IS)< maintenance " in the "user manual" for
64-3 Note internal
liquid 80.0mmol/l 或 the detailed
abnorma l 140.0mmol/ <
C(IS)。
(3) Please re-execute ISE calibration.
(2)current IS
calibration
result is not
updated
Because of the
implementatio
n of the ISE
maintenance
(ISE cleaning,
complete Executive ISE calibration
cleaning), it is
necessary to
implement ISE
calibration
155
Alarm Detailed
Description Solution
Code description
solution:
156
Alarm Detailed
Description Solution
Code description
solution:
157
Alarm Detailed
Description Solution
Code description
solution:
158
Alarm Detailed
Description Solution
Code description
159
Alarm Detailed
Description Solution
Code description
160
Alarm
Code Detailed
Description Solution
description
solution:
161
Alarm
Code Detailed
Description Solution
description
16-1 solution:
162
Alarm Detailed
Description Solution
Code description
solution:
163
Alarm
Code Detailed
Description Solution
description
solution:
164
Malfunction 1: Electromagnet stops or fails to
leave the top
solution:
165
9.16 Resetting and others
Alarm
Code Detailed
Description Solution
description
Sending time
Time
synchronizati Check the communications of control board and
143-1 synchronizat
on order sub-boards respectively.
ion failure
failure
Water tank Check whether the water supply machine, solenoid
Water liquid system valve, pipeline and filter are working normally, and
adding malfunction, check whether the water supply pipe has air, and
143-2
overtime water adding check whether the floater is normal, and check
error overtime error whether relevant electrical units of floater and control
board are normal.
AD board
detects
Use control panel monitoring software to monitor the
malfunction
AD board fault information to see if there is AD communication
143-3 when
reset failure error. Repair AD board if there is AD communication
resetting, AD
error to debug.
board reset
failure。
Reaction
disk detects
malfunction
Reaction when resetting, Lower machine monitoring software monitors error
143-4 disk reset information, and analyze the reaction disk
Reaction
failure disk reset board-related failure to eliminate the error
failure。
Sample disk
detects
Sample disk malfunction Use lower machine monitoring software to monitor
143-5 reset when error information, and refer to the related information
failure resetting, of sample disk board to debug.
sample disk
fails to reset。
R1 disk detects
malfunction
when Use lower machine monitoring software to monitor
R1 disk resetting, R1
143-6 error information, and refer to the related error
reset failure disk fails to information of R1 board to debug.
reset
R2 disk detects
malfunction
when Use lower machine monitoring software to monitor
R2 disk resetting, R2
143-7 error information, and refer to the related error
reset failure disk fails to
reset information of R2 board to debug.
Check whether the indication of reaction bath liquid
Water Water
level detector is right; check whether there is
dischargin dischargin
remaining water in reaction bath, and check reaction
143-8 g failure of g failure of
bath water outlet pipeline if there is remaining water
reaction reaction
in it; check whether relevant electrical units of liquid
bath bath
level detection and control board are normal.
166
R1 and R2
Connect the main control board debugging program
reagent
to electrify the machine; observe machine electrifying
probes fails
Adding flow; observe whether the 6 times the normal
to add
detergent cleansing movement of detergents reagent 1 reagent
143-9 detergent
overtime 2 is finished in the phase of adding detergents.
completely
error Corresponding control board of reagent probe should
in the
be repaired if the reagent probe did not achieve
specified
normal movement.
time
R1 fails to
R1liquid
detect liquid
level
143-10 level when Refer to liquid detection error analysis to debug
detection
adding
failure
detergent
R2 fails to
R2 liquid
detect liquid
level
143-11 level when Refer to liquid detection error analysis to debug
detection
adding
failure
detergent
Liquid level Check the liquid level of reaction bath, and check
Liquid level
detection whether the liquid level detector of reaction bath
detection
143-12 failure of works normally; check whether relevant electrical
failure of
reaction units of liquid level detection and control board are
reaction bath
bath normal.
167
Reaction Reaction Use lower machine monitoring software to monitor
143-17 disk stop disk stop error information, and refer to the related error
failure failure information of reaction disk to debug.
Previous Previous
Use lower machine monitoring software to monitor
sample sample
143-19 error information, and refer to the related error
adding adding
information of sample disk to debug.
failure failure
Previous Previous
Use lower machine monitoring software to monitor
reagent 1 reagent 1
143-20 error information, and refer to the related error
adding adding
information of reagent 1 to debug.
failure failure
Previous Previous
Use lower machine monitoring software to monitor
reagent 2 reagent 2
143-21 error information, and refer to the related error
adding adding
information of reagent 2 to debug.
failure failure
Previous Previous
Use lower machine monitoring software to monitor
reagent 3 reagent 3
143-22 error information, and refer to the related error
adding adding
information of reagent 2 to debug.
failure failure
168
waste liquid bottle and control board are normal.
Switch
malfunction, Check whether the floaters of high and low liquid level
high-level and the signal cable connecting floater to main
143-29 Floater floater control board are normal; check whether relevant
switch error detects the electrical units of floater detection and control board
signal, but are normal.
low-level
floater fails.
Reagent Reagent
level level Use lower machine monitoring software to monitor
143-30 scanning scanning error information, and refer to the related error
overtime overtime information of reagent board to debug.
error error
Sample Sample
barcode barcode Use lower machine monitoring software to monitor
143-32 scanning scanning error information, and refer to the related error
overtime in overtime in information of sample board to debug.
testing testing
ISE detects
Observe whether there is ISE alarm information;
malfunction
ISE reset Use lower machine monitoring software to monitor
143-33 in resetting;
failure error information, and refer to the related error
resetting
information of ISE board to debug.
failed.
ISE check
ISE check Observe whether there is ISE alarm information; Use
maintenanc
maintenance lower machine monitoring software to monitor error
143-34 e
movement information, and refer to the related error information
movement
overtime of ISE board to debug.
overtime
ISE pipeline
ISE pipeline rinsing is Observe whether there is ISE alarm information; Use
143-35 rinsing over lower machine monitoring software to monitor error
overtime specified information, and refer to the related error information
time of ISE board to debug.
ISE
ISE malfunction Observe whether there is ISE alarm information; Use
143-36 malfunction when testing, lower machine monitoring software to monitor error
in testing follow-up ISE information, and refer to the related error information
stops of ISE board to debug.
169
Check gear pump and pressure sensor of sample
pipeline are normal. Check whether the read value of
pressure sensor which is running is more than 2500
Gear pump Gear pump
143-37 by using main control board debugging software;
failure pressure low
check whether relevant electrical units of pressure
sensor and control board are normal.
Sending
reagent
Sending
mapping
reagent
information
143-42 mapping Refer to instrument module error analysis
failure,
information
adding
failure
reagent may
fail.
When the
water tank
temperature
is over 36.5
degrees, add
cold water
Execute the main board debugging program to
into the tank
observe whether the display of temperature is normal
into to cool
cooling and room temperature is high; check the water supply
down.
143-43 water pipeline of water tank is normal; check whether
Temperature
overtime relevant electrical units of temperature sensor and
could not
control board are normal.
drop to 35.5
degrees in
one minute,
which means
cold water
temperature
is too high.
Execute the main board debugging program to
Analyzer Malfunction monitor whether the communications of main board
143-44 module among and sub-boards are normal; check whether relevant
malfunction modules electrical units of sub-boards communications and
control board are normal.
Check whether light source, water quality of
Continuous
Continuous incubation bath, reaction cup and counting light
emergence
143-45 emergence sensor of reaction disk are normal; test data
of 5 dirty
of dirty cups cups collecting board lines by using the testing program of
data collecting board.
AD data AD data Execute the main board debugging program to
malfunction mixed monitor whether the communications of main control
143-46
board and AD board is normal, and analyze error;
check whether counting light sensor is normal.
IES ISE Use lower machine monitoring software to monitor
malfunction measuring error information, and refer to the related error
143-47 in testing internal information of ISE board to debug.
standard
liquid failure
Execute the main board debugging program to
Version Version
monitor whether the communications is normal, and
number number
143-48 analyze error; check whether relevant electrical units
reading reading
of sub-boards communications and control board are
overtime overtime
normal.
170
9.17 Cooling system
Alarm
Code Detailed
Description Solution
description
Cooling system Cooling time (2) observe the temperature displayed on digital
144-1
abnormal abnormal pipe of cooling system and Peltier current value are
normal.
The 1st line The 1st line Check the 1st line cooling Peltier and cooling chip
145-1 cooling chip cooling
malfunction current <5A
The 2nd line The 2nd Check the 2nd line cooling Peltier and cooling chip
145-2 cooling chip line cooling
malfunction current <5A
The 3rd line The 3rd line Check the 3rd line cooling Peltier and cooling chip
145-3 cooling chip cooling
malfunction current <5A
The 4th line The 4th line Check the 4th line cooling Peltier and cooling chip
145-4 cooling chip cooling
malfunction current <5A
171
9.18 AD collector
Alarm Detailed
Description Solution
Code description
The 1st line The Check the AD collection board and preamp board
AD collector measuring
malfunction value of the
146-1 1st line AD
collector is
over normal
range
The 2nd line The Check the AD collection board and preamp board
AD collector measuring
malfunction value of the
146-2 2nd line AD
collector is
over normal
range
The 3rd line The Check the AD collection board and preamp board
AD collector measuring
malfunction value of the
146-3 3rd line AD
collector is
over normal
range
The 4th line The Check the AD collection board and preamp board
AD collector measuring
malfunction value of the
146-4 4th line AD
collector is
over normal
range
The fifth line The Check the AD collection board and preamp board
AD collector measuring
malfunction value of the
146-5 fifth line AD
collector is
over normal
range
172
The sixth line The Check the AD collection board and preamp board
AD collector measuring
malfunction value of the
146-6 sixth line AD
collector is
over normal
range
The seventh The Check the AD collection board and preamp board
line AD measuring
collector value of the
146-7 malfunction seventh line
AD collector
is over
normal range
The eighth The Check the AD collection board and preamp board
line AD measuring
collector value of the
146-8 malfunction eighth line
AD collector
is over
normal range
The ninth line The Check the AD collection board and preamp board
AD collector measuring
malfunction value of the
146-9 ninth line AD
collector is
over normal
range
The tenth The Check the AD collection board and preamp board
line AD measuring
146-10 collector value of the
malfunction tenth line
AD collector
is over
normal range
The 11th line The Check the AD collection board and preamp board
AD collector measuring
malfunction value of the
146-11 11th line AD
collector is
over normal
range
The 12th line The Check the AD collection board and preamp board
AD collector measuring
malfunction value of the
146-12 12th line AD
collector is
over normal
range
173