Weekly Progress Report

Students Name: Sabijon Agnes Grace D.

Week Number:
Inclusive Dates: October 03, 2018

Crime Scene Investigation (Polymerase Chain Reaction)

 Activites:

 First, 5 tubes and 5 X 0.2 mL PCR tubes were kept on ice during the procedure. We used
aerosol barrier pipet tips and either adjustable micropipette set to 20microliter or a fixed
volume of 20microliter pipet and 20microliter DNA were added to each tube.
 We used the adjustable micropipette and added 20microliter of the Master’s mix and primers
to each tube. The contents on the PCR tubes were mixed by gently pipetting up and down.
Once the MMP was added to the tube, the cap was closed. The solution of the PCR tubes was
blue.
 The cap of our PCR tubes were placed in their adaptors on ice and instructed to place our
tubes on the thermal cycler.
 For the lesson two, the gel electrophoresis apparatus was set up. The 5 PCR reactions were
placed capless into a rack.
 We added 10microliter of orange G loading dye to each PCR reaction tube and it was mixed
well. 20microliter of the allele ladder was loaded to each sample of the gel.
 The gel was run at 100V for 30mins and it was stained in fast blast DNA stain as directed.
 After is the staining of the agarose gel, the overnight staining and the quick staining which is
the protocol 1 and protocol 2.
 Lastly, the cellophane sandwich and plastic container method was conducted for the gathering
of results. Through the process of DNA profiling and with the addition of reagents, we
identified who is the murderer when doing a crime scene investigation.

Noted by:

Christine Anna-Lou M. Lomboy

Chairman- Biology Department, Velez College