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Sterilization of Water
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The common sterilizer agents are chlorine, ozone,
bleaching powder, potassium per magnate chloramines.
Sterilization of water through bleaching powder gives
chlorine and if it is in excess, it is harmful for health
and causes diseases like airway irritation, wheezing,
sore throat, cough, chest tightness etc,
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General Methods of Sterilizing
Water
Physical Methods:
1. Boiling the water:
Boiling is one of the oldest and most commonly used water
sterilization techniques. If the water is boiled for about 20-
30 minutes then it destroy all type of harmful
bacteria and algae. They also remove dissolved gases from
the water and improve the water taste. This method is only
suitable for normal households but not suitable for
industrial and large scale purposes.
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2. UV sterilization:
Ultra-violet light has a powerful germicidal action on water.
It is one of the latest methods for sterilization of water.
When ultraviolet radiation is passed through the water
then it is absorbed by the cells and damages the DNA of
the microorganisms. Hence they are not enable to grow or
reproduce. This physical sterilization method can be
applied for groundwater and households water but not
suitable for large scale. It does not create solid content in
water. The water should be free from suspended particles
otherwise it may interfere to come in UV rays contract with
microorganisms. The system is costly.
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Chemical Methods:
1.Ozone sterilization of water:
Ozone is a highly effective sterilization chemical for water.
It has strong oxidizing properties. Ozone is more powerful
disinfectant than chlorine or chlorine dioxide. Moreover it
has effective inactivation activities on giardia or
cryptosporidium which is not possible by some others
chemicals. This method does not affected by pH. After
complete the oxidation process, the excess ozone will
breaks into oxygen. So it does not create bad taste or odor’s
to the water and leaves no solid residue. On the contrary,
it added dissolved oxygen into the water which enhances
the water’s taste. The disadvantages of ozone are that it is
costly, toxic, unstable and must be produced on mill site.
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2.Chlorine sterilization:
Chlorine is the most widely used disinfectant in municipal
water and wastewater treatment. It can destroy pathogens
and control nuisance microorganisms. It can also remove
iron, manganese, ammonia nitrogen. Chlorine is a toxic gas
so need safety facilities. It acts as a quick oxidizing agent.
pH can affects on its activities so needs to control the pH.
Chlorine reacts with water and form hypochlorous acid
which is further break into nascent oxygen. Both of them
are powerful germicide.
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1.Burette 2. Titration Flask
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Bleaching powder
Na2S2O3
KI
Different Samples of Water
Distilled Water
Soluble starch
Indicator - Starch Solution
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Preparation of N/20 Na2S2O3 solution :
Take 12.4 gm of sodium thio-sulphate hydrated and
mix it in about 500ml of water then the mixture is
diluted to make the volume 1000ml. or 1lt.
Normality = strength/Molecular Mass.
= 12.4 / 248 = 1/20N
Preparation of 10%KI solution:
Take 25gm. of KI powder and mix it in about 100ml of
water then dilute the mixture to make the volume 250
ml and take it in the measuring flask.
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Preparation of Bleaching Powder solution :
Weight 5gm of bleaching powder and mix it in about
200 ml of distilled water in a conical flask. Stopper the
flask and shake it vigorously. The suspension thus
obtained is filtered in measuring flask of 500ml. and
then dilute the filtrate with water to make the volume
500 ml. The solution obtained is 1% bleaching powder
solution.
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in a beaker with constant stirring. Boil the contents for
4-5min. and then allow to cool.
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Titration : I (Distilled Water)
Volume of distilled water taken = 100ml
Volume of bleaching powder solution taken = 20ml
Volume of KI solution added = 20ml
Burette Reading
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Titration : II (Underground Water)
Volume of water sample I taken = 100ml
Volume of bleaching powder solution added = 20ml
Volume of KI solution added = 20ml
Burette Reading
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Titration : III (Tank Water)
Burette Reading
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Titration : IV (Contaminated Water)
Burette Reading
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Sample I (Underground Water)
Amount of bleaching powder used to disinfect 100ml of
water sample I.
= (8.0 - 5.1) ml of 0.2 N of Na2S2O3 solution = 2.9ml.
1ml of bleaching powder solution contains bleaching
powder =(5/500)gm = 0.01gm.
20ml of bleaching powder solution = 8.0ml of 0.2N of
Na2SO4
So 1ml of Na2S2O3 solution = 20/8 ml of bleaching
powder solution.
Volume of bleaching powder solution used to disinfect
100ml of water sample = (2.9 x 20/8)ml.
(2.9 x 20/8)ml of bleaching powder solution contains (2.9
x 20 x 0.01 gm / 8)gm bleaching powder.
Amount of bleaching powder required to disinfect 1 litre
of water = (2.9 x 20 x 0.01 x 1000) / (8 x 100) = 0.725gm
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Sample II (Tank Water)
Amount of bleaching powder used to disinfect 100ml of
water sample II.
= (8.0 - 5.5) ml of 0.2 N of Na2S2O3 solution = 2.5ml.
1ml of bleaching powder solution contains bleaching
powder =(5/500)gm = 0.01gm.
20ml of bleaching powder solution = 8.0ml of 0.2N of
Na2SO4
So 1ml of Na2S2O3 solution = 20/8 ml of bleaching
powder solution.
Volume of bleaching powder solution used to disinfect
100ml of water sample = (2.5 x 20/8)ml.
(2.5 x 20/8)ml of bleaching powder solution contains (2.5
x 20 x 0.01 gm / 8)gm bleaching powder.
Amount of bleaching powder required to disinfect 1 litre
of water = (2.5 x 20 x 0.01 x 1000) / (8 x 100) = 0.625gm
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Sample III (Contaminated Water)
Amount of bleaching powder used to disinfect 100ml of
water sample III.
= (8.0 – 2.9) ml of 0.2 N of Na2S2O3 solution = 5.1ml.
1ml of bleaching powder solution contains bleaching
powder =(5/500)gm = 0.01gm.
20ml of bleaching powder solution = 8.0ml of 0.2N of
Na2SO4
So 1ml of Na2S2O3 solution = 20/8 ml of bleaching
powder solution.
Volume of bleaching powder solution used to disinfect
100ml of water sample = (5.1 x 20/8)ml.
(5.1 x 20/8)ml of bleaching powder solution contains (5.1
x 20 x 0.01 gm / 8)gm bleaching powder.
Amount of bleaching powder required to disinfect 1 litre
of water = (5.1 x 20 x 0.01 x 1000) / (8 x 100) = 1.275gm
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Amount of the given samples of bleaching powder
required to disinfect one litre of water :-
1.275
1.5
0.625
1 0.725
SAMPLE III
0.5 SAMPLE II
SAMPLE I
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AMOUNT OF
BLEACHING
POWDER TO
DISINFECT 1L
OF SAMPLE
(gm)
SAMPLE I SAMPLE II SAMPLE III