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To receive full credit for Exercise 5, you must submit the following:
1) A typed Report Form (available from Moodle). Be certain to complete all calculations,
tables, and figures and answer all questions fully using complete sentences.
2) A completed Illustration Form (handed out in class and available from Moodle) for the
graph.
3) A partial scientific report consisting of an Introduction and Conclusion section for Part III
– Osmosis Experiment. Use the guideline for writing a scientific report in the beginning
of your lab manual to help you produce these two sections.
Purpose of Lab:
To observe the movement of solutes through water and the movement of water through cell
membranes in response to different solute concentrations
Develop a prediction regarding whether dye will diffuse faster at hot or cold
temperatures; indicate why you think this will happen:
Start time
End time
Questions:
2. How does concentration gradient change over time?
4. Which bottle has the area with the highest concentration, and how does this relate to
concentration gradient?
5. If you stir the water and food dye with a spoon, what color would the water be? Would
you expect to see a concentration gradient? How does stirring, or mechanical mixing,
differ from diffusion?
Questions:
1. Which solution is hypertonic relative to the solute concentration of the egg? What does
the egg in the hypertonic solution look like? In which direction is the net movement of
water?
2. Which solution is hypotonic relative to the egg? What does the egg look like? In which
direction is the net movement of water?
Prediction
Starting weight
Final Weight
Final Weight -
Starting Weight
% Change
(formula on
previous page
Notes and
Observations
Table 5.4: Percent change in weight of potatoes treated with different solutions.
Distilled H2O
0.5% NaCl
1.0% NaCl
1.5% NaCl
2% NaCl
2.5% NaCl
Questions: Use the class-wide group data to answer the following questions.
1. What happened to the potato in the distilled water? Did its weight change at all? If so,
did it increase or decrease?
3. Was the 2.5% NaCl solution hypotonic, isotonic, or hypertonic to the potato?
4. Which of the five solutions do you think was closest to being isotonic to the potato
cells? Why do you draw this conclusion?
5. Predict how the weight of the potato would change if you took the cubes you just used
and placed them into different solutions, as follows?
-Place the cube from the distilled water into 2.5% salt water:
-Place the cube from the 2.5% salt water into distilled water:
-Place the cube from the 1.5% salt water into distilled water:
-Place the cube from the 1.5% salt water into 2.5% salt