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Department of Education
Region III
City Division of San Jose del Monte
CITY OF SAN JOSE DEL MONTE NATIONAL SCIENCE HIGH SCHOOL
RESEARCH TITLE:
An Ingredient for Mosquito Repellant
Liquid Soap: Lemongrass (Andropogon
Citratus / Cymbopogon) and Basil
(Ocimum basilicum)
S.Y. 2018-2019
ABSTRACT
The real danger of mosquitos laid their abilities to transmit diseases such as
malaria, and dengue. People had used many tools to control the spread of mosquitos in
different places, especially at the houses. Researchers use a lemongrass and a basil for
their research. The two said variable were the main ingredients to be use in
genus of Asian, African, Australian, and Tropical Island plants in the grass family.
of food in terms of cookery. Lemongrass also contains citronellal or rhodinal, the main
component in the mixture of terpenoid chemical compounds that give citronella oil its
distinctive lemon scent, which can be used as medicinal herb for asthmatic patients
attending a specialty care clinic. Basil also called great basil or Saint-Joseph's-worth, is
a culinary herb of the family Labiatae (mints). Basil is native to tropical regions from
central Africa to Southeast Asia. It is a tender plant, and is used in cuisines worldwide.
Depending on the species and cultivar, the leaves may taste somewhat like anise, with
a strong, pungent, often sweet smell. Basil is called by many names like sweet basil or
even Thai basil, but all of its common names refer to the herb's botanical name,
Ocimum basilicum.
ACKNOWLEDGEMENT
We would like to acknowledge the help of each member’s residence for having a place
to gather and plan. The cooperation of each members for a successful research. Also,
by the help of Savemore Muzon for completing our materials in research. Lastly, the
help of San Jose del Monte National Science Highschool’s laboratory that allows us to
Research Problem:
The real danger of mosquitos laid their abilities to transmit diseases such as
malaria, and dengue. People had used many tools to control the spread of mosquitos in
different places, especially at the houses. The most commonly used repellent is
composed of strong chemicals that can harm an individual, most of these can kill
mosquitos but they can also do a serious damage to human as well. We decided to
make a new product that is as similar as to other repellent. The product that we will
conduct is not only affordable, but also safe to use, not likely in chemical, which is
Tropical Island plants in the grass family. Lemongrass is commonly used as a special
ingredient or spices to enhance the aroma of food in terms of cookery. Lemongrass also
contains citronellal or rhodinal, the main component in the mixture of terpenoid chemical
compounds that give citronella oil its distinctive lemon scent, which can be used as
about 2 m (6.6 ft.) and have magenta-colored base stems. These species are used for
the production of citronella oil, which is used in soaps, as an insect repellent (especially
mosquitos) in insect sprays and candles, and in aromatherapy. The principal chemical
constituents of citronella, geranial and citronellal, are antiseptics, hence their use in
household disinfectants and soaps. Besides oil production, citronella grass is also used
ward off insects such as whitefly adults. Lemongrass (Cymbopogon) is a group of about
caffeic acid, all of which help in providing an impressive range of medicinal aids. The
main component of this fragrant herb is lemonal or citral, which has anti-fungal and
family Labiatae (mints). Basil is native to tropical regions from central Africa to
the species and cultivar, the leaves may taste somewhat like anise, with a strong,
pungent, often sweet smell. Basil is called by many names like sweet basil or even Thai
basil, but all of its common names refer to the herb's botanical name, Ocimum
basilicum. Basil is a member of the large mint family, or Labiate family, along with other
labs. In cooking, basil is most commonly used fresh in cooked recipes. More often than
not, the fresh leaves are added at the last moment, as cooking quickly destroys the
herb's distinct flavor. But today as also seen throughout history, basil is not only used as
a food flavoring, but also in perfumery, incense, and herbal holistic remedies. Recent
scientific studies have established that compounds the essential oil of basil plants
Justification:
The world wide’s one biggest issue is the rapidly spreading of dengue virus
carried by mosquitoes that which of transmitted to humans that lead to their death.
According to the study of WHO (World Health Organization), 26,433 people that been
affected as of April 1, 2017 were suspected cases of dengue reported from January 1,
2017. Many researchers tried their best to conduct a research regarding on how to
lessen the cases of Dengue. Lemongrass and basil are some of the best repellent they
have seen so far which it is non-hazardous to human use. The two plants that have
been mentioned earlier have an aromatic scent that drives away mosquitoes from
Students
The research can provide students insight about the effect of common organic
household insect repellent such as liquid soap that affect the disease rate of dengue
and other mosquito-related diseases. Their knowledge will be enhance and widen all
about science that is related to this study. These can be a guide for them to learn and
help them to observe how common organic household insect repellent such as liquid
soap can reduced the rate of mosquito-related diseases and its importance.
Society
soap gives us idea specifically people on rural areas who disposed their stocked water
everywhere and also in secluded areas which are prone to mosquitoes. This may give
diseases.
The research gives the conclusion that the concentration of essential oils in the
product which is insect repellent liquid soap may affect the duration of the effectiveness
of it. The authentic concentration of the essential oils is very important because it can
have a positive or negative effect to the human. It will give us idea especially to the
future researchers that Lemongrass and basil essential oils are as effective as the
Research Questions:
What are the components of the lemongrass and basil that makes it possible to
repel mosquitoes?
How long does this soap last in repelling a mosquito? Can it be used in long
ranges?
What are the procedures in making lemongrass and basil mosquito repellent
liquid soap?
How safe is the lemongrass and basil mosquito repellent liquid soap to use?
The scope of this study was only limited by using two plants that has the ability to
repel mosquitoes. Our main objective is to lessen the rate of having a dengue here, in
the Philippines. This study covers how lemongrass and basil can repel mosquito in the
For the purpose of clarification, the important terms used in the study have been
defined.
essential oils.
inflammatory,
cutworms or armyworms).
This chapter presents the review of related literature of the research. Each
variable were discussed with giving brief description on it. Moreover, the researchers
Literature Review
According to the study of S. Moore and M. Maia (2011) in” WHO Pesticide
Nowhere are these knowledge on traditional repellent plants were obtained throughout
ethno botanical studies is a sole resources for the development of new natural products.
evaluate repellent compounds and develop new products that offer high repellency as
The advancement of insect repellent science shows how chemicals can influence
well as exploring novel repellent chemistries throughout the year. (G. Paluch, L.
covered with scales as are the veins of the wings. Mosquitoes are also characterized by
long, fragile-looking legs and elongated, piercing mouthparts. The feathery antennae of
the male are generally bushier than those of the female. The males, and sometimes the
females, feed on nectar and other plant juices. In most species, however, the females
require the proteins obtained from a blood meal in order to mature their eggs. Different
Severe human tropical diseases such as malaria, dengue, yellow fever and
fever which is a viral infection caused by several Flavivirus species whose most
important vector is Aedes aegypti L. Dengue and dengue hemorrhagic fevers threaten
an estimated 2.5 billion people or almost 50 million people per year. Another serious
tropical disease is filariasis or elephantiasis which threatens about 1 million people over
80 countries. Filariasis is caused by infections by several roundworm species of which
Wuchereria bancrofti Cobbold is the most essential and is transmitted by the common
feet high (in gardens, occasionally somewhat taller), with a short, but irregular, crooked,
much-branched stem, covered with a yellowish-grey bark, which comes off in flakes,
finely pubescent, with stellate hairs. Quantification of phenol acids was determined
accessions. Chemical studies revealed the rosmarinic acid is the predominant phenolic
acid present in Basil’s stem and leaf tissues. (A. Kashi, et.al, 2002)
tropical grasses in the Poaceae (grass family) for their production of aromatic essential
oils, including citronella oil (from C. nardus) and geranial (from C. martini), used in
perfume and as natural insecticides. The species C. citratus, lemongrass, is widely used
in Asian cooking to flavor soups, stews, and curries (including Vietnamese lemongrass
soup), as well as meat, fish, and poultry dishes (including Indonesian “satay”); it is also
used in herbal teas. Cymbopogon species originated in the Old World tropics, and
generally form dense clumps with wide blades. Different species grow from 0.5 to 2 m
(2 to 6 ft) tall. The fleshy leaf bases (pseudo stems) are harvested to be used in cooking
or to be pressed for oil. Some species are also used in agroforestry for soil
The use of herbal preparations remained the main approach of folk medicine to
inquiry into the efficacy of the tea. Lemongrass tea contains several bio compounds in
its decoction, infusion and essential oil extracts. Anti-oxidant, anti-inflammatory, anti-
Lemongrass tea was non-toxic, non-mutagenic and receives wide acceptance among
Overall, based on the related literature given above, the two plants: Lemongrass
(Cymbopogon) and Basil (Ocimum basilicum) have a big similarity regarding their
chemical composition for which they have an aromatic scent that drives away the
insects such as mosquitoes. The two plants have the common ground which is phenolic
acid that functions as insect repellent due to its natural smell which negates the
Research-Related Literature
are vectors of parasitic and viral diseases of immense importance for public health. The
acquisition of the genome sequence of the yellow fever and Dengue vector, Aedes
repertoire: in Aedes aegypti, the malaria vector Anopheles gambiae, and the fruit fly
modules reveals both conservative and rapidly evolving features associated with
different functional gene categories and particular aspects of immune reactions. These
Since dengue virus spreads fast all over the world, millions of people are on risk
regarding to this phenomenon. The dengue was one of the leading causes of death
according to WHO. A synthetic compound has been the most effective single repellent
for mosquito species and is the basis for many commercial repellent products on the
insects. They are the sources of the natural insecticidal and larvicidal substances
nicotine (Nicotiana L.), quassin (Quassia amara L.), rotenone (Derris sp.) pyrethrins like
chrysanthamic acid and its derivatives present in pyrethrum and azadirachtin. These
and other natural insect control agents have served as the basis for the development of
insecticides and piperonyl butoxide synergist. Also, pyrethroids and piperonyl butoxide
synergism are the basis for a number of commercially available mosquito control
nardus. They have been used in mosquito repellency and are the most widely used
natural repellents today. Also Eucalyptus essential oils (Eucalyptus l’her spp.) are
widely used to repel insects, including mosquitoes, and contain insecticidal and
(Kholi, 2006) Citronella essential oil, Eucalyptus essential oil and other plant oils
registered as safe and effective ingredients for use in topical insect repellents. However,
mosquito bites was tested. For each test, 10 disease-free, laboratory-reared Aedes
aegypti female mosquitoes that were 7 to 24 days old were placed into separate
had not been exposed to the repellent being tested was used for each arm insertion.
Mosquitoes were provided with a constantly supply of 5% sucrose solution. Cages were
temperature was maintained at 24 to 32 °C, the relative humidity at 60 to 70%, and the
(5 men and 10 women) were recruited from the staff of the Medical Entomology
Laboratory at the University of Florida. Subjects conducted their first test of each
repellent by inserting the treated arm into a test cage for 1 minute every 5 minutes. If
they were not bitten within 20 minutes, then the arm was reinserted for 1 full minute
every 15 minutes, until the first bite occurred. On the basis of this initial complete-
protection time, the subject's next two tests of that particular repellent were conducted
as follows: if the repellent had initially worked for less than 20 minutes, the subject
placed his or her arm in the cage for 1 minute every 5 minutes; if the repellent had
initially worked for 20 minutes to 4 hours, the subject placed his or her arm in the cage
for 1 minute every 15 minutes; and if the repellent had initially worked for more than 4
hours, the subject placed his or her arm in the cage for 1 minute every hour (up to 4
hours). If a repellent was still working after 4 hours, then the subject continued to place
his or her arm in the cage every 15 minutes thereafter, until the first bite occurred. If at
any point during testing, subjects noted mosquitoes landing but not biting (a behavior
that typically occurs when the efficacy of a repellent begins to wane), then the intervals
The study concludes that mosquitoes landing on the surface of the skin but not
biting on it. The study also concludes that the repellent still good and effective until 4
hours. Only products containing DEET offer long-lasting protection after a single
Overall, based on the related studies given above, the researchers have an
insight on how does the Lemongrass and Basil will be as effective as commercial DEET
repellents. This will be the guide on them on how could the experimentation and
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