The Annihilation Effect of Cane Toad (Bufo marinus), Bufotoxin Crude Extract Against

Rice Case worm (Paraponyx stagnalis)

Research Plan

A. Statement of the Problem

This study will primarily aim to determine the Annihilation Effect of Cane Toad (Bufo

marinus) Bufotoxin Crude Extract Against Rice Case worm (Paraponyx stagnalis)

Specifically, it will seek to answer the questions below.

1. What treatment generated the best result in terms of:

a. number of dead rice case worm; and

b. number of alive rice case worm

2. Is there any significant difference between the five treatments in terms of the number

of dead rice case worm?

B. Hypothesis

The study will be guided by the hypotheses below.

1. Treatment 4 (100 % Bufotoxin Crude Extract) generated the best result in in terms

of:

a. number of dead rice case worm; and

b. number of alive case worm

2. There is a significant difference between the five treatments in terms of the number

of dead rice case worm.

C. Experimental Design

This study will be laid out using Complete Randomized Design (CRD).
D. Data Gathering Procedure

To be able to gather the data needed in this study, the researcher will do the procedures

below.

1. Preparation of Test Area

Before conducting the study, the test area will be cleaned to maintain the cleanliness

of the area. Two (2) glass box and five (5) containers will be prepared for the experiment. The

containers will be labeled T1, T2, T3, T4, and T5.

2. Preparation of Test Subjects

a. Cane Toads

Five (5) cane toads will be collected randomly at Barangay Nagbalioartian Sinait

Ilocos Sur. The researcher will use gloves and eyeglass for protection. The collected cane toad

will be placed in a glass box.

b. Rice Caseworm

Twenty-five (25) rice caseworm will be collected at the farm at Barangay

Nagbalioartian, Sinait, Ilocos Sur. The researcher will use gloves for protection. Net will be used

in collecting the rice caseworms. The collected rice caseworms will be placed in a glass box.

Five (5) caseworms will be randomly selected and placed in every container.
 3. Bufotoxin Crude Extract Secretion

The researcher will bring the Cane Toads to Vigan City. The Bufotoxin Crude Extract

will be secreted by the experts.

4. Treatment Preparation

The concentrations below will be used by the researcher as treatments of the study.

Treatment Concentration

Treatment 1 25% Bufotoxin Crude Extract and 75% Water

Treatment 2 50% Bufotoxin Crude Extract and 50% Water

Treatment 3 75% Bufotoxin Crude Extract and 25% Water

Treatment 4 100% Bufotoxin Crude Extract

Treatment 5 Control

5. Pest Management Activity

The treatments will be sprayed to the designated container and will be observed twenty-four

hours (24). After the experiment, the researcher will count the number of dead rice caseworm

and record the data collected.

E. References

Wang, G.; G. Sun; et al. (1994). "The application of traditional Chinese medicine to the
management of hepatic cancerous pain". J Tradit Chin Med. 14(2): 132–138. PMID 7967697
Lincoff, Gary; Mitchel, Duane H. (1977). Toxic and Hallucinogenic Mushroom Poisoning: A
Handbook for Physicians and Mushroom Hunters. New York: Van Nostrand Reinhold. ISBN
978-0-442-24580-1
Rice caseworm". Rice Knowledge Bank. Retrieved 11 March 2017