HPLC

SOLVENTS
ENABLE BETTER DETECTION AND
SEPARATION OF CHEMICAL COMPOUNDS

Put reliability in your laboratory

 Five
HPLC techniques give best results for impurities
profile and purity verification

HPLC solvents are:

• Free from UV absorbing impurities to ensure there are no interfering Peaks
• Controlled acidity and alkalinity
• Low in moisture for better performance in normal phase cromatography
• Ensure high reproducibility and accuracy in results
• Minimum baseline noise in gradient analysis
• Filtered through 0.2 micron filers to enhance longevity of the column
• Filtered through 0.2micron filters to enhance longevity of the column
• Accurate peak quantification
• High resolution and detection of trace impurities
• Processed with rigorous quality checks to ensure batch to batch consistency
• Available in small and replaceable large packs

Hence, HPLC solvents are

Appropriate for your analysis and HPLC Systems

Also Available
in 4 Litre
Glass Bottle
 Five
HPLC solvents are ideally suited for :
• Sample preparation
• Method development
• Preparation chromatography
• Analytical application

Condition
Detector : PDA
HPLC Column : C-18, 5micron, 250mm x 4.6mm
Mode : Gradient
Mobile Phase : Water HPLC/Acetonitrile HPLC
Method : 0-100% ACN over 24min (V/V)
Wavelength : 205nm and 254nm
Flow Rate : 1.8ml/min

Gradient Analysis
0.10 Time (min)
Wavelength 205 nm
0.08
0.06
au

0.04
0.02
0.00
-0.02
0.00 2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00 18.00 20.00 22.00 24.00

0.10 Time (min)

0.08 Wavelength 254 nm
0.06
0.04
au

0.02
0.00
-0.02
0.00 2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00 18.00 20.00 22.00 24.00
 Five
Trouble Shooting through advantage

HPLC Solvent
Advantages
Sequential distillation through 30
feet column to remove interfering
and reactive compounds

Novel chemical treatment for

removing UV and fluorescence
sensitive impurities.

The water content is controlled

and measured by series of Karl
Fischer Titrations, performed
at various stages
 Five
Product Information RANKEM on HPLC Solvents
Product Name Min.Assay Water By KF NVM UV Cut off Min UV transmittance

(nm) Wavelength (nm) Transmittance

Acetic Acid NLT 99.8% Max. 0.2% Max. 0.001% 252 252 nm 10%
Acetone NLT 99.8% Max. 0.1% Max. 0.0003% 330 330 nm 15%
Acetonitrile NLT 99.8% Max. 0.03% Max. 0.0005% 190 200 nm 90% (0.046)*
Acetonitrile Gradient Gold HPLC NLT 99.9% Max. 0.01% Max. 0.0001% 190 195 nm 80%
Acetonitrile Gradient HPLC NLT 99.9% Max. 0.03% Max. 0.0004% 190 200 nm 93% (0.032)*
Acetonitrile HPLC NLT 99.8% Max. 0.03% Max. 0.0005% 190 200 nm 90% (0.046)*
Acetonitrile preparative HPLC NLT 99.8% Max. 0.05% Max. 0.005% 190 220 nm 85%
Benzene NLT 99.8% Max. 0.05% Max. 0.0005% 280 280 nm 10%
Benzyl Alcohol NLT 99.8% Max. 0.1% NA NA 290 nm 30%
Iso - Butyl Alcohol NLT 99.5% Max. 0.05% Max. 0.001% 220 220 nm 10%
Tert - Butyl Alcohol NLT 99.5% Max. 0.1% Max. 0.0005% 212 215 nm 10%
Carbon Tetrachloride NLT 99.8% Max. 0.01% Max. 0.0005% 265 270 nm 45%
Chloroform NLT 99.0% Max. 0.05% Max. 0.0005% 245 250 nm 35%
Cyclohexane NLT 99.7% Max. 0.01% Max. 0.0005% 200 230 nm 50%
Dichloromethane NLT 99.7% Max. 0.05% Max. 0.0005% 233 235 nm 30%
Diethyl Ether NLT 99.8% Max. 0.03% Max. 0.0005% 215 220 nm 10%
Dimethyl Sulphoxide NLT 99.8% Max. 0.1% Max. 0.001% 268 270 nm 30%
1, 4 Dioxan NLT 99.5% Max. 0.05% Max. 0.0005% 215 225 nm 20%
Ethyl Acetate NLT 99.8% Max. 0.05% Max. 0.0005% 256 260 nm 30%
Ethyl Methyl Ketone NLT 99.5% Max. 0.1% Max. 0.0005% 329 330 nm 10%
Ethylene Dichloride NLT 99.8% Max. 0.03% Max. 0.005% 228 230 nm 10%
n-Heptane NLT 99.0% Max. 0.03% Max. 0.0005% 195 200 nm 10%
Hexane NA Max. 0.01% Max. 0.0005% 195 210 nm 25%
Methanol NLT 99.8% Max. 0.05% Max. 0.0005% 205 210 nm 25%
Methanol Gradient Gold HPLC NLT 99.9% Max. 0.02% Max. 0.0001% 205 210 nm 35%
Methanol Gradient HPLC NLT 99.8% Max. 0.05% Max. 0.0005% 205 210 nm 30%
Methanol HPLC NLT 99.8% Max. 0.05% Max. 0.0005% 205 210 nm 25%
Methyl Tert - Butyl Ether NLT 99.8% Max. 0.05% Max. 0.0005% 210 220 nm 20%
n - Hexane NLT 99.0% Max. 0.02% Max. 0.0005% 195 200 nm 20%
N, N – Dimethyl formamide NLT 99.8% Max. 0.05% Max. 0.0005% 268 275 nm 50%
n-Butyl Alcohol NLT 99.7% Max. 0.1% NA 215 215 nm 10%
Iso - Octane NLT 99.7% Max. 0.01% Max. 0.0005% 215 210 nm 20%
Ortho Phosphoric acid NLT 85.0% NA NA NA 210 nm 85% (0.07)*
n-Pentane NLT 99.5% Max. 0.005% Max. 0.0005% 195 200 nm 20%
Petroleum Ether 40 - 60 NLT 99.0% Max. 0.02% Max. 0.0001% 210 210 nm 20%
Propan - 1 - ol NLT 99.8% Max. 0.05% Max. 0.0005% 210 210 nm 20%
Propan - 2 - ol NLT 99.7% Max. 0.1% Max. 0.0005% 205 210 nm 20%
Tetrahydrofuran NLT 99.7% Max. 0.03% Max. 0.001% 220 245 nm 25%
Toluene NLT 99.8% Max. 0.03% Max. 0.0005% 284 285 nm 10%
Triethylamine NLT 99.8% Max. 0.08% Max. 0.003% 275 330 nm 60%
Water HPLC NA NA Max. 0.0003% <190 210 nm 0.004
 Five
Use Rankem buffers and ion pairing reagents
along with rankem HPLC solvents
For :

• Consistent retention time

• Best resolution
• No UV absorbing impurity
• No insoluble impurity

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Deventer, the Netherlands 9001:2008 & 14001:2004 & 13485:2003 Mumbai, India 9001:2008 & 17025:2005

About Avantor™ Performance Materials

Avantor Performance Materials manufactures and markets high-
performance chemistries and materials around the world under several TM

respected brand names, including the J.T.Baker®, Macron Fine Chemicals™,

Rankem™, BeneSphera™ and POCH™ brands.

Avantor products are used in a wide range of industries. Our biomedical

and life science solutions are used in academic, industry and quality control
laboratories for research, pharmaceutical production and medical lab testing,
while our electronics solutions are used in the manufacturing of semiconductors.

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