ROM 6004 00 Altair Software Service Manual PDF

Random Access Analyzer
Service Software Manual

for Instrument Calibration
DAR# 16.0403 ROM.G6004.00 EFF Date: 04/19/16

Altair™ 240 – Service Software Manual Section i
INTRODUCTION
Section i INTRODUCTION
i.1 Identification Data

This is the manual for the BCServer software used, during service operations, to verify and/or
calibrate the Altair™ 240 system (random access analyzer).
This document must be considered in addition to the instrument Service Manual used for service
operations. Service is performed on the instruments only by authorized and qualified technical
personnel trained by the Manufacturer.
Service engineers and maintenance technicians must carefully read every section of this manual
and of the Service manual before undertaking any operation.
This manual is meant to be used as a reference aid; technicians need to have been trained by
authorized personnel.
The Manufacturer is not responsible for partial and unauthorized copies of this document.
This manual has been written and produced with the utmost care; however errors cannot be fully
excluded.
The Manufacturer is not responsible for incidents that may occur from mistakes found in the
manual.
The service technician must contact the distributor or the Manufacturer in case of doubt.
i.1.1 Document
 Document code: G6004
 Document revision: .00
 Revision date: March 22, 2016
i.1.2 Instrument
 Altair™ 240: Ref # G6000-001

(Without ISE Module)
 Altair™ 240: Ref # G6600-001
(With ISE Module)
i.1.3 Manufacturer
 EKF Diagnostics
1261 N. Main
Boerne, Texas 78006 U.S.A.
Phone: 830.249.0772
Fax: 830.249.0851
E-mail: techsupport@ekfdiagnostics.com
WEB: www.ekfdiagnostics.com
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INTRODUCTION
i.2 Copyright
The content of this document, pictures, tables and the graphics included, are the intellectual
property of EKF Diagnostics.
Unauthorized copies, total or partial, could cause legal actions in order to preserve owner’s
interests.
i.3 Purpose of This Document

This document is the manual that describes commands, functions and controls of the “BCServer”
service interface. The program BCServer, that manages the instrument, provides a visual interface
for verification and calibration of the instrument itself.
It is meant for service engineers and qualified technicians trained by the Manufacturer or by
authorized personnel (in the following named as “operator”) to perform service operations on the
system.
i.4 Use of This Manual

Manufacturer recommends that service technicians carefully read all sections of this manual
paying particular attention to notes. These notes specify or re-inforce a concept just discussed and
warnings, used to highlight possible risks or dangers. Technicians should be familiar with the User
Manual and the Service Manual.
Notes and warnings are written in bold.
Note: For any information regarding Theory of Operation, Functions, Performances, Operating
Procedures and User Interface Software Menu Descriptions, refer to the User Manual and to the
Service Manual – latest revisions.
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INTRODUCTION
i.5 List of Contents

Section i INTRODUCTION ........................................................................................................................... 2
i.1 Identification Data ................................................................................................................................... 2
i.1.1 Document ......................................................................................................................................... 2
i.1.2 Instrument ......................................................................................................................................... 2
i.1.3 Manufacturer ................................................................................. Error! Bookmark not defined.
i.2 Copyright ................................................................................................................................................... 3
i.3 Purpose of This Document ...................................................................................................................... 3
i.4 Use of This Manual .................................................................................................................................... 3
i.5 List of Contents .......................................................................................................................................... 4
Section 1 SOFTWARE APPLICATION FOR SERVICE .................................................................................... 5
1. General ...................................................................................................................................................... 5
1.1. Legend for Symbols Adopted in This Section ............................................................................. 5
1.2. Command and Functions - Information ..................................................................................... 5
1.3. Client and Server Software ............................................................................................................ 7
1.3.1. Starting BCClient and BCServer ............................................................................................... 7
1.3.2. BCServer Software ...................................................................................................................... 9
1.3.3. Configuration and Control Panels......................................................................................... 10
1.3.3.1. Master Sensors – Acquired by the Main Controller board ....................................... 12
1.3.3.2. ARM1 Sensors – Acquired by the ARM1 Controller board ....................................... 14
1.3.3.3. ARM2 Sensors – Acquired by the ARM2 Controller board ....................................... 16
1.3.3.4. RW Sensors – Acquired by the R&W Controller board ............................................. 18
1.3.4. Calibration Panels ..................................................................................................................... 20
1.3.4.1. ARM1 Position Panel ........................................................................................................ 22
1.3.4.2. Samples and Reagents Panel ....................................................................................... 32
1.3.4.3. ARM2 Position Panel ........................................................................................................ 36
1.3.4.4. Temperature Control Panel ........................................................................................... 37
1.3.4.5. Reading Test Panel .......................................................................................................... 41
1.3.4.6. Washing Station Panel .................................................................................................... 48
1.3.4.7. Cycle Control Panel ........................................................................................................ 52
1.3.4.8. Cycle Timings Panel ........................................................................................................ 55
1.3.4.9. Cycle Status Panel ........................................................................................................... 55
1.3.4.10. Status Panel ...................................................................................................................... 56
1.3.4.11. ISE Control Panel ............................................................................................................. 58
1.3.5. BCServer window ...................................................................................................................... 64
1.3.6. Parameters Download ............................................................................................................. 66
1.4. MSTool – Service SW Tool for Altair Instruments ........................................................................ 68
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Altair™ 240 – Service Software Manual Section 1
SOFTWARE APPLICATION FOR SERVICE
Section 1 SOFTWARE APPLICATION FOR SERVICE

1. General
This section explains the use of BCServer program for instrument calibration, inspection and
verification.
1.1. Legend for Symbols Adopted in This Section

Example Name and Function
Text or one character written with this font shows text or characters
to be digit by keyboard.
MONOSPACE
This font has been used for name of hard disks, paths, folders and
programs.
The text shows software fields and commands to be selected (i.e.:
menu or pop-up possible selections). The text also indicates name
Bold
for parameters, controls and buttons into the front panel, windows,
sections, menu and palettes’ name.
This symbol leads through nested menu and through the dialog
windows up to the final action.
» I.e.: the sequence file»page Setup»Options advise the operator to
start from menu File, to choose Page Setup and then to choose
Options in the last dialog window.
<Testo> The text inside points a key to be pressed on the keyboard.
Indicates to press two or three keys on the keyboard at the same
<Testo> + <Testo>
time.
Indicates to press more keys on the keyboard in cascade (respect
<Testo> , <Testo>
the order).

Indicates important notes or sentences.
1.2. Command and Functions - Information

Example Type Name and function
HOME. Send motor to Home position.
Key
Position Control. Indicates the number of steps that the
motor must run from HOME position.
Clicking with the left mouse button on the GO TO POSITION
Field
command the motor moves the number of steps in the
field. That value can be introduced/modified by the
keyboard.
Go To Position. This command moves the motor for the
Key
number of steps shown in the Position control.
RESET. This command resets the involved assembly by
Key
checking also home positions.
General Control Field. It shows the value associated to a
Field motion or to a parameter. It can be modified by
keyboard.
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Example Type Name and function

MEMORIZE. It stores in memory the value shown in the
Key
Position Control or in the related General Control.
MEMORIZE X AND Y. It stores in memory the value shown in
Key
the Position Control of the related motor.
CHECK BUTTON. It allows the selection of one of more
Check values.
Button
MEMORIZE GAP. It stores in memory the value shown in the

Key Position Control of the related motor or in the Check
Button of the related Diluter.
Activation LED. It activates the associated peripheral,
generally shown into the label on the right. LED can be ON
(enabled) or OFF (disabled):
Led
Led OFF
Led ON
Check. It allows the selection of a parameter. It can
Check generally be FALSE or TRUE. The check sign value is TRUE
and the parameter is taken under consideration.
Display. It shows a value read by the instrument; this
Display control is not modifiable by the operator. Its purpose is the
visualization of a parameter/value.
Tree View. It’s a menu that opens to the right. Generally
the tree is closed.
The presence of the sign “+” reveals the presence of a
sub-menu.
The presence of the sign “-” indicates that the sub-menu is
open.
Tree A mouse click on the sign “+” opens the sub-menu.
View After checks have been done, always close the active
menu.
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1.3. Client and Server Software

The application BCClient.exe (herein after called Client) is the User Interface: it can be entered
introducing user name and password.
The application BCServer.exe (herein after called Server) is the program managing the instrument
and scheduling all of its activities; it also includes the Service software interface. This program is
always running in background and it is not visible to the user. It can be open by the service
operator through special and reserved user name and password to be considered “confidential”.
1.3.1. Starting BCClient and BCServer
Operation Name and Function

To start the User Interface software of the system,
click the icon in the desktop.
In normal installations the BCServer starts and works in

background; it also runs the serial communication
from PC to the instrument.
During initialization, the User Interface software (BC

Client) opens the Password window that requires
entering of user credentials.
Enter in the text fields the following strings:
User name: admin

Password: 12345678
And then press <Enter>.
If codes entered are correct,

the BCClient user software starts showing the Main
menu.
After initialization, on the bottom right side of the

Windows menu bar it’s included the icon (small black
“pipette”) of the software BCServer. It indicates that
BCServer is active and back-ground running.
Click on it with the right mouse button to access the
program BCServer and follow the instructions below
for entering. or
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Operation Name and Function

After the right click on the small “pipette” icon, the
dialog window BCServer Login opens.
Introduce the following strings:
User: serviceadmin
Password: adminservice
Then click OK or press Enter.
If user and password are correct, the BCServer,

software service interface, opens and the monitor
will show three main windows (and one message
window – reserved – in the back ground).
To exit the BCClient use the following procedure: in the BCClient main panel click ShutDown»Yes
and wait for software shutdown; the BCClient will automatically close also the BCServer. Use this
procedure to exit BCServer when the Client is running; this procedure could anyway be not
always useful during maintenance. As an alternative, during testing, BCServer can be run in
another way. From Windows explorer run the application BCServer.exe under the following path:
C:\BCA\Comunication\Exe.
Only BCServer will start (not the BCClient – User Interface); to show the dialog window BCSever
Login, follow then the procedure given above.
In the case BCServer has been run directly from Windows Explorer; it is necessary to close it ONLY by
closing the lower window named BCServer (that’s the only way to shut it down) through the
Note: To shut down the BCServer ONLY, click on the “red X” on the top right
of the “BCServer” window in the bottom – never close the other windows.
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1.3.2. BCServer Software

Configuration Calibration
and Control Panels.
Panel.
This set of
This window windows allows
allows the complete
instrument instrument
sensor status calibration. It is
checking. possible to send
commands to
motors, to verify,
to read and to
modify
calibration
parameters.
BCServer.
It graphically shows reading cuvette zeros (transparent values
updated for each cuvette after any washing at all wavelength).
Service Messages.
During working this window is hidden behind the main three ones.
It shows some of the PC/instrument handshaking messages.
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1.3.3. Configuration and Control Panels
2
1
3
1
Client configuration and Control.

Do not use this section.
1 Do not modify parameters within this software section (highlighted in red).
1 Any modification could lead to malfunctioning due to default parameter change.
Ignore this section.
Cycle configuration and Control.
2 This section allows technician to enter the Sensors Test tree menu.
1
Sensors Test.
This menu and its submenus allows instrument sensor status acquisition (Home Position,
3 obstacles and level sensors …) given only by the section “Sensor Status”, ignore the other
1 menus.
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It is possible to enter Sensors Test sub-menus by

clicking on “+”.
The sub-menus is composed by four branches: each
of them refers to one of the four Controller boards of
the instrument:
Master Sensors: Main Controller board

Arm1 Sensors: Arm1 Controller board
Arm2 Sensors: Arm2 Controller board
RW Sensors: Read and Wash Controller
board
The list of sensors under monitoring related to the

board selected is shown on the right side of the
window. Each sensor is represented by a LED.
Do not use nor modify the following control panels:

 Master Cycle Control
 Master RW Control
 Cycle Errors
Changes could cause instrument malfunctioning.
Ignore them for service.
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1.3.3.1. Master Sensors – Acquired by the Main Controller board
1
1
2 3
1 1
4
1
5
1
6
1
7
1
Cover sensor (protection defense).
The Led shows the instrument cover status.
Cover CLOSED.
1
1 Cover OPEN.
In case that the sensor connector isn’t plugged or in case the contact is discontinuous, Led
is ON: Cover Open.
Home RGT.
Reagent tray Home Position sensor (optocoupler).
Reagent tray NOT in Home position.

2
1 Reagent tray IN Home position.
is ON: Reagent tray IN Home position.
Home SMP.
Sample tray Home Position sensor (optocoupler).
Sample tray NOT in Home position.

3
1 Sample tray IN Home position.
is ON: Sample tray IN Home position.
Reached Minimum Liquid 1 Level (20Lt tank - Systemic Solution).
Systemic solution tank floating level sensor.
Systemic solution tank NOT empty.

4
1
Systemic solution tank Empty.
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To check the sensor it is sufficient to unplug/plug the connector with full tank. In case the
sensor connector isn’t plugged or in case the contact is discontinuous, Led is ON: Systemic
Solution tank empty.
Reached Minimum Liquid 2 Level (5Lt tank - Cleaner Solution).
Cleaner solution tank floating level sensor.
Cleaner solution tank NOT empty.
5
Cleaner solution tank Empty.
1
sensor connector isn’t plugged or in case the contact is discontinuous, Led is ON: Cleaner
Solution tank empty.
Reached Minimum Cooling Liquid Level.
6 No longer used
1 Not used in this system.
Reached Maximum Discharge Liquid Level (25Lt - Waste tank).
Waste tank floating level sensor (Waste).
Waste tank NOT full.
7
Waste tank FULL.
1
sensor connector isn’t plugged or in case the contact is discontinuous, Led is ON: Waste
tank full.
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1.3.3.2. ARM1 Sensors – Acquired by the ARM1 Controller board
1 2 3
1 1 1
4
1
5
1
Home X.
ARM1 X-axis home position sensor (optocoupler).
ARM1 NOT in Home X position.

1
1
ARM1 IN Home X position.
In case the home sensor connector isn’t plugged or in case the contact is discontinuous, Led
is ON: ARM1 IN Home X position.
Home Y.
2 ARM1 Y-axis home position sensor (optocoupler).
1
ARM1 NOT in Home Y position.
ARM1 IN Home Y position.
is ON: ARM1 IN Home Y position.
Home Dil.
3 ARM1 Diluter Home Position sensor (optocoupler).
1
ARM1 diluter NOT in Home position.
ARM1 diluter IN Home position.
is ON: ARM1 Diluter IN Home Y position (top of head).
Capacitive Sensor.
4
ARM1 capacitive liquid level detector.
1
The LED is ON when the ARM1 probe touches the liquid into reagent bottles, sample tubes,
etc.
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Liquid level NOT detecting.
Liquid level DETECTING a capacitive variation.
At probe capacitance variation (i.e.: touch with probe support your finger) the RED Led on
the Liquid Level Detector board goes ON: ARM1 Liquid level DETECTED. Also the LED on the
submenu goes ON after some seconds.
In case of continuous or intermittent liquid detection on the board, without any capacity
variation the sensor is not correctly working or some liquid is between the probe and its
support.
The Liquid Level sensor detects temporary capacitance variations – you can check it by
temporary touching the probe support with your fingers (pay attention – probe can be
contaminated).
Obstacle Sensor.
5 ARM1 Y obstacle optocoupler sensor (Crash sensor).
1 The LED is ON when the ARM1 probe detects an obstacle along Y-axis way down.
Obstacle NOT detected.
Obstacle DETECTED.
is ON: ARM1 Obstacle DETECTED.
The obstacle sensor, activated by sliding up the probe, detects a crash just after 5 times – one
per second – when in Idle status: to check it keep it up for more than 5 seconds – help with
gloves (pay attention, wear gloves – probe must be considered contaminated).
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1.3.3.3. ARM2 Sensors – Acquired by the ARM2 Controller board
Home X.
ARM2 X-axis home position sensor (optocoupler).
ARM2 NOT in Home X position.

1
1
ARM2 IN Home X position.
is ON: ARM2 IN Home X position.
Home Y.
2 ARM2 Y-axis home position sensor (optocoupler).
1
ARM2 NOT in Home Y position.
ARM2 IN Home Y position.
is ON: ARM2 IN Home Y position.
Home Dil.
3 ARM2 Diluter Home Position sensor (optocoupler).
1
ARM2 diluter NOT in Home position.
ARM2 diluter IN Home position.
is ON: ARM2 Diluter IN Home Y position (top of head).
Capacitive Sensor.
4
ARM2 capacitive liquid level detector.
1
The LED is ON when the ARM2 probe touches the liquid into reagent bottles, sample tubes,
etc.
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Liquid level NOT detecting.
Liquid level DETECTING a capacitive variation.
At probe capacitance variation (i.e.: touch with probe support your finger) the RED Led on
the Liquid Level Detector board goes ON: ARM2 Liquid level DETECTED. Also the LED on the
submenu goes ON after some seconds.
In case of continuous or intermittent liquid detection on the board, without any capacity
variation the sensor is not correctly working or some liquid is between the probe and its
support.
The Liquid Level sensor detects temporary capacitance variations – you can check it by
temporary touching the probe support with your fingers (pay attention – probe can be
contaminated).
Obstacle Sensor.
5 ARM2 Y obstacle optocoupler sensor (Crash sensor).
1 The LED is ON when the ARM2 probe detects an obstacle along Y-axis way down.
Obstacle NOT detected.
Obstacle DETECTED.
is ON: ARM2 Obstacle DETECTED.
The obstacle sensor, activated by sliding up the probe, detects a crash just after 5 times –
one per second – when in Idle status: to check it keep it up for more than 5 seconds – help
with gloves (pay attention, wear gloves – probe must be considered contaminated).
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1.3.3.4. RW Sensors – Acquired by the R&W Controller board
1 2 3
1 1 1
4
1
Home CVT.
Cuvette wheel Home Position sensor (optocoupler).
Cuvette wheel NOT in home position.

1
1
Cuvette wheel IN home position.
is ON: Cuvette wheel IN Home position.
Home FLT.
2 Filter wheel Home Position sensor (optocoupler).
1
Filter wheel NOT in home position.
Filter wheel IN home position.
is ON: Filter wheel IN Home position.
Home Wash.
3 Washing station Home Position sensor (optocoupler – higher washing station position).
1
Washing station NOT in Home Position.
Washing station IN Home Position.
is ON: Washing station IN Home position (high).
WARNING:
IMPORTANT, IF THE LED IS OFF, WASHING STATION NEEDLES HEAD COULD BE INSIDE CUVETTES;
BEFORE PROCEEDING MOVE NEEDLE HEAD TO THE TOP, HIGH POSITION.
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Washing Low Sensor.

4
Washing station Low Position sensor (optocoupler – lower washing station position, bottom of
1
cuvettes).
Washing station NOT in Low Position.
Washing station IN Low Position.
is ON: Washing station IN Low position (inside cuvettes).
WARNING:
IMPORTANT, IF THE LED IS OFF, WASHING STATION NEEDLES HEAD COULD BE INSIDE CUVETTES;
BEFORE PROCEEDING MOVE HEAD TO THE TOP, HIGH POSITION.
WARNING:
IMPORTANT, IF BOTH LED: Home Wash e Washing Low Sensor, ARE OFF, THE WASHING STATION
NEEDLES HEAD COULD BE IN AN INTERMEDIATE POSITION, BEFORE PROCEEDING PUT HEAD IN THE TOP
HIGH POSITION AND CHECK LED OPERATION.
IMPORTANT, IF BOTH LED: Home Wash e Washing Low Sensor, ARE ON, THE WASHING STATION
SENSORS ARE NOT WORKING, BEFORE PROCEEDING CHECK BOTH OPTOCOUPLER SENSORS.
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1.3.4. Calibration Panels
1
2
3 1
1
4
5 1
1 6
7 1
1
8
9 1
1 10
11
In order to open one of the panels above it is necessary to click on the bar with the wished name.
More windows open at the same time could require manual resize.
Arm1 Position Panel.
Within this panel it is possible to drive and calibrate the sampler ARM1 in all positions. It is also
1 possible to move the ARM1 diluter plunger and to send commands for activation of the ARM1
Diluter Pump, of Washing Sink Pump, of ARM1 Electrovalve; moreover it allows the heater coil
temperature power regulation (actually not used).
Samples and Reagents Panel.
2 Within this panel it is possible to drive the Sample Wheel and the Reagent Wheel and to read
1 from barcode. By association with Arm1 Position Panel or with Arm2 Position Panel it is
possible to move the ARM1 or ARM2 on Sample and Reagent tray different positions.
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Arm2 Position Panel.

Within this panel it is possible to drive and calibrate the sampler ARM2 in all positions. It is also
3 possible to move the ARM2 diluter plunger and to send commands for activation of the ARM2
Diluter Pump, of Washing Sink Pump, of ARM2 Electrovalve; moreover it allows the heater coil
temperature power regulation (if needed).
Temperature Control Panel.
Within this panel it is possible: to introduce target incubation temperature and related limits, to
4
enable bar-code reader and ISE Module, to regulate Warming Up times, to set dilution
1
volumes, etc.
Reading Test Panel.
5
Within this panel it is possible: to calibrate each filter position (Filter wheel), to read any cuvette
1
absorbances and plot counts vs. time values, to move and calibrate cuvette wheel in reading
position.
Washing Station Panel.
6 Within this panel it is possible: to calibrate Washing Station positions, to activate dispensing and
1 suction pumps and also to control their activation times and power.
Cycle Control Panel.
7 Within this panel it is possible: to set general parameters used by the instrument during
1 operation.
Cycle Timings Panel.
8
This panel is reserved – NOT USED. No meaning for instrument calibration.
1
Cycle Status Panel.
9
This panel is reserved – NOT USED. No meaning for instrument calibration.
1
10 Status Panel.
Within this panel it is possible to show the instrument status and eventual alarm/warning
generated by the BCClient.
11 ISE Control Panel.
Through this panel it is possible to calibrate, to check and to send command to the ISE
Module.
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1.3.4.1. ARM1 Position Panel
Arm1 Position Panel includes 3 separate sections: X positions (rotation), Y position

(elevation), D position (Diluter). Each of them refers to a single motion of the ARM1 assy.
X Position section.
1 7
3 8
4 9
5 10
External (position on Reagent).

Reagent #1 position.
This Check Button, when selected, enables the visualization of ARM1 X-position when probe is
on reagent bottle #1. The selection of this button disables the others.
In the Y Position field is visualized the related ARM1 Y-axis coordinate (in motor steps).
1 X-position: this is reagent bottle entering X-position; it must be calibrated with sampling probe
in the center of the reagent bottle inlet.
Y-position: this is the bottom protection Y-coordinate (limit); it must be calibrated when the
probe is about 1mm (13 steps) over the bottom of the reagent bottle. Higher coordinate gives
higher reliability but also higher dead volume.
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Washing (inner and outer washing position of the sampler probe).

2 Position for probe washing into the washing sink; just in front of the side nozzle for outer surface
probe washing.
This Check Button, when selected, enables the visualization of ARM1 X-position coordinate
when probe is in Washing Sink. Selection of this button disables the others.
In the Y Position section on the right, there is a field for the visualization of the ARM1 Y-axis
coordinate (in motor steps).
X-position: this is the probe washing X-position; it must be calibrated with the sampler probe in
the center of the washing nozzle aside (pipe outlet for washing systemic solution pumped by
the probe peristaltic pump) – the solution, when flowing, must wet the entire diameter of the
probe. This X-coordinate is equal to the Wait/Travel position X-coordinate.
Y-position: this is the probe washing Y-position. The Washing Y-position must be set after
calibration of the Wait/Travel Y-position (see further on). It is set in the following way: find the
Y-coordinate for the Wait/Travel Y-position and add 90 steps, that’s the Washing Y-position
coordinate to memorize.
Washing sink
Nozzles
Wait/Travel and
Washing X-position
When this Washing Check Button is activated, more commands related to the Washing
position are displayed; they are explained below.
Diluter EV.
A Click on Diluter EV LED to switch
ON/OFF THE electrovalve.
A D
OFF – Electrovalve CLOSED
B C
ON – Electrovalve OPEN.
WARNING: B
Diluter Pump.
Click on Diluter Pump to
IN CASE OF DILUTER PUMP ACTIVATION, STRICTLY RUN/STOP the Diluter Pump
FOLLOW THIS MANUAL SEQUENCE TO TURN ON (probe inner surface washing).
ARM1 DILUTER PUMP:
OFF – Diluter Pump OFF
1. WASH PUMP (well sinking)
2. DILUTER EV (Electrovalve) ON - Diluter Pump ON.
3. DILUTER PUMP (probe rinsing) Wash Pump.
C Click on Wash Pump LED to
TO SWITCH OFF THEN PUMP, FOLLOW THE REVERSE RUN/STOP the Washing Sink
STEP ORDER; TURN OFF WASH PUMP ONLY WHEN Draining Pump.
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WASHING SINK IS EMPTY.
(Avoid to turn ON the Diluter Pump with Diluter OFF – Washing Sink Pump OFF
Pump EV in OFF as the water cannot flow and
internal pressure will increase too much damaging ON - Washing Sink Pump ON.
the hydraulic circuit). Probe Pump.
D Click on Probe Pump to RUN/STOP
the side nozzle probe Pump
(probe outer surface washing).
OFF – Washing Sink Pump OFF
ON - Washing Sink Pump ON.
Cuvette Wheel.
Position for probe dispensing into the cuvette.
in cuvette wheel. Selection of this button disables the others.
In the Y Position field it is visualized the related ARM1 Y-axis coordinate (in motor steps).
X-position: this is the cuvette entering X-position. It must be calibrated with sampler probe in
the center of the cuvette below WHEN the cuvette tray has been previously calibrated and
positioned with cuvette #1 under the first needle pair of the washing station (see later on in
this document).
Y-position: this is the bottom protection Y-coordinate (limit); it must be calibrated when the
probe is about 2.5mm over the bottom of the cuvette to allow good mixing and to avoid
bubble generation; it means: this position must be about 35steps over the point in which the
sampling probe just starts touching the bottom of the cuvette – this can be recognized by
looking at the obstacle flag rising up. Alternatively you can use our special tool – a calibrated
metallic cuvette – for easier calibration: insert the special metal tool in place of cuvette #1
3 and just notice (by sending down the probe step by step) when the red liquid level detector
lamp activates “ON”: that’s the correct coordinate for calibration (in case of low LLD
sensitivity, use the former method).
When this Check Button is activated, more commands related to the Cuvette wheel are
displayed; they are explained in the following.
A B
C
Cuvette No.
A Introduce the cuvette number into this field and the Position field (“B”) will
automatically show the number of motor steps associated to that position. This value
ranges from 0 to 80: don’t introduce negative values.
Position.
B
When the check button is selected, this field shows the number of motor steps
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associated to the cuvette number introduced in the field Cuvette No. It shows the
number of steps that cuvette wheel must rotate for positioning that specified cuvette
under ARM1 probe. It is possible to modify this value and it is possible to introduce
negative values.
In case of negative value, if you want, you can also adopt the following older rule (nr. of
step  nr. introduced):
……………
11  11
……………
2  2
1  1
0  0
-1  65535
-2  65534
……………
-12  65524
……………
Introduce values by keyboard.
Home.
C
Click this key to move Cuvette Wheel in Home Position.
Go To Position.
D
Click this key to move the Cuvette Wheel so that the cuvette number specified in the
field Cuvette No is positioned under ARM1 probe (dispensing position). The number of
motor steps are those shown in the position control field Position.
Never move the cuvette tray with sampler probe into the cuvette – it will be damaged.
Sample Wheel.
Position for probe sample aspiration (tubes or sample cups).
on sample positions #1. Selection of this button disables the others.
In Y Position field is visualized the related ARM1 Y-axis coordinate (in motor steps).
X-position: this is sample tube/cup entering X-position; must be calibrated with sampling
4 probe in the center of the hole.
Y-position: this is the sample tube/cup bottom protection Y-coordinate (limit); must be
calibrated when the probe is about 1.5/2.5mm over the bottom of the sample cup/tube. It is
possible to calibrate three possible Y-coordinate: sample cup, sample tube and cap piercing.
Sample cup is for OD=12mm 3mL cups (no adapter required), Sample tube is for primary tubes
(OD=12-13mm by H=75-100mm), Cap piercing is for special applications and it’s used only in
special custom versions.
Empty.
5 This position is NOT USED in this system – ignore it.
No calibration is required.
Wait / Travel.
The travel position is used by the system to temporary wash the sampler probe end between
the reagent aspiration and the sample aspiration.
It must be calibrated so that the probe can wash the outer surface for about 3-4mm from its
6 end through the side nozzle. This coordinate must be taken with the sampler probe in the
center of the washing nozzle aside. From the coordinate found it is possible to deduct the
Washing Y-position by adding 90 steps.
X-position: this is the probe travel X-position; it must be calibrated with the sampler probe in
the center of the washing nozzle aside (pipe outlet for washing systemic solution pumped by
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the probe peristaltic pump) – the solution, when flowing, must wet the entire diameter of the
probe end. This X-coordinate is equal to the Washing position X-coordinate.
Y-position: this is the Y-coordinate; it must be set in the following way: find the Y-coordinate
where the probe-end just touches the low side of the washing sink crossing slot and record it.
Add exactly 16steps to the recorded coordinate and memorize it; that’s the Wait/Travel Y-
position coordinate to save.
Wait/Travel and
Washing X-position
Sampler probe
Calibration of the
Wait/Travel position:
Record ARM Y-position when
the probe-end is aligned with
the washing station bottom of
crossing slot and then
Subtract 16 steps.
Alignment on the
bottom of the slot
Washing sink
Home.
Click this key to send ARM1 X-Axis in Home Position.
7 WARNING:
ACTIVATING THIS COMMAND, THE INSTRUMENT RUNS X-AXIS HOME POSITION WITHOUT ANY
8 CONTROL ON Y-AXIS POSITION SO: BEFORE TO RUNNING THIS COMMAND BE SURE THE ARM1 Y-
AXIS IS IN HOME POSITION ITSELF.
Position.
When the check button is selected, this field shows the number of motor steps, stored into the
instrument or in software memory, that are associated to the Check Button selection. It shows
the number of steps that the ARM1 must perform in order to move the probe on the position
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selected with the Check Button. It is possible to modify this value and it is possible to introduce
negative values.
In case of negative value, if you want, you can also adopt the following old rule (nr. of step 
nr. introduced):
……………
11  11
……………
2  2
1  1
0  0
-1  65535
-2  65534
……………
-12  65524
……………
Go To Position.
9 Click this key to move ARM1 X-Axis to the position selected with the Check Button. The number
of motor steps are those shown in the position control field Position.
Memorize X and Y.
This key allows memorization at the same time of the X-Axis and Y-Axis positions selected by
the Check Button.
10
WARNING:
ACTIVATING THIS COMMAND, THE INSTRUMENT TEMPORARY SAVES MODIFIED PARAMETERS IN
THE PC MEMORY; PARAMETERS MUST BE DOWNLOADED TO THE INSTRUMENT TO BE OPERATIVE.
THE PROCEDURE FOR PARAMETER DOWNLOADING IS DESCRIBED LATER IN THIS DOCUMENT.
IF MODIFIED PARAMETERS ARE NOT DOWNLOADED INTO THE INSTRUMENT, THEY WILL BE LOST AT
BCSERVER PROGRAM SHUT DOWN-AND THE OLD PARAMETERS ARE NOT MODIFIED.
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Y Position section in case of “sample” selected.
1
2
5
10
3
6
4
9 8 7
Home.
1
Click this key to send ARM1 Y-Axis in Home Position.
Sample Cup.
2 This command is active only if Sample has been selected on the left side of the panel.
When selected, this Check Button enables the visualization in the field Position of the low
limit ARM1 Y-coordinate when probe is on a sample cup. Selection of this button disables the
others.
Y-position: this is the sample cup bottom protection Y-coordinate (limit); must be calibrated
when the probe is about 1.5/2.5mm over the bottom of the sample cup.
In Position field is then visualized the very bottom low position that never will be surpassed by
the probe: it’s a protection coordinate placed on the bottom of sample cup. Higher
coordinate gives higher reliability but also higher dead volume.
Sample Tube.
3 This Check Button, when selected, enables the visualization in the field Position of the low
limit ARM1 Y-position when probe is on a primary tube type (12-13 diameters). Selection of this
button disables the others.
Y-position: this is the sample tube bottom protection Y-coordinate (limit); must be calibrated
when the probe is about 1.5/2.5mm over the bottom of the sample tube.
In Position field it is visualized the very bottom low position that never will be surpassed by the
probe: it’s a protection coordinate placed on the bottom of sample tube. Higher coordinate
gives higher reliability but also higher dead volume.
Cap piercing.
4 This Check Button is RESERVED. Y-position: Y for Cap piercing is for special applications and it’s
used only in special custom versions. Calibration is not required – do not modify it.
Position.
5
When the check button is selected, this field shows the number of motor steps associated to
the Check Button selection. It shows the number of steps that ARM1 must perform in order to
move the probe on the position selected with the Check Button. It is possible to modify this
value: never introduce negative values.
Go To Position.
6
Click this key to move ARM1 Y-Axis to the position selected with the Check Button. The number
of motor steps are those shown in the position control field Position.
Memorize.
7
This key allows memorization of the value Heat % that is not used. Do not activate it as not
used.
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Heat %.
8
Reserved - Actually not used. Leave it equal to 0 (zero) – no Calibration required.
Disp (0,1%).
9
Reserved - Actually not used. Leave it equal to 1 (one) – no Calibration required.
Reset.
10
This command runs a complete Reset of ARM1 assembly (Y-axis, X-axis and Diluter motors).
It runs first the Home X and then Home Y, it moves into Washing Sink and initiate an inner and
outer probe washing in the Washing position (electrovalve, Diluter pump and Probe pump); at
the end, during the way up to Home Y, it performs Diluter Home and sets the final air gap.
Y Position section in case of “reagent” selected (3-selection opportunity: currently under

development)
2
3
1
7 6 5
Home.
1
See same command explanation given in the previous table.
R1.
2 This command is active only if Reagent has been selected.
This Check Button, when selected, enables the visualization in the field Position of the low
limit ARM1 Y-coordinate when probe is on/in any type of reagent bottle.
Y-position: this is the Reagent bottle bottom protection Y-coordinate (limit); must be
calibrated when the probe is about 1.0/1.5mm over the bottom of the bottle (choose the one
with the “higher” bottom between “lower” and “higher” volume bottles).
In Position field is then visualized the very bottom low position that never will be surpassed by
the probe: it’s a protection coordinate placed on the bottom of bottle. Higher coordinate
gives higher reliability but also higher dead volume.
Position.
3
4 Go To Position.
Memorize.
5
Heat %.
6
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Disp (0,1%).
7
Diluter Position section.
1
1 5 6
2
1 1
1 7
1
3 8
1 1
9
11
4 1
1 10
Home.
1
Click this key to send the ARM1 Diluter in Home Position.
1
Go To Position.
2
Click this key to move the ARM1 Diluter to the position indicated in the lower white field
1 Absolute Position (µL). The number of motor steps are those shown in the upper grey field.
The value is automatically converted into “µL” by the software in the lower white field – the
conversion, and then the resolution, depends on the correct type of diluter used by the
instrument and set into the Opportunity.ini file; that file includes a section for the specification
of diluter resolution given in µL/step and maximum capacity (given in µL) – that setting is
performed by the installation program.
Absolute Position (µStep).
3 This field shows the number of motor steps related to fluid volume to be aspirated/dispensed
1 by the diluter that are indicated in the field Absolute Position (µL). This value is not
modifiable.
Absolute Position (µL).
4 This field shows the volume in microliters (µL) to be aspirated/dispensed by the diluter. The
1 Absolute Position (µStep) field shows the steps correspondent to the volume. It is possible to
delete this value and to introduce it by keyboard. The value must be included between 0 and
500µL, it is not possible to introduce negative values. Steps-to-microliters conversion is
performed by the software.
Relative Position (µStep).
5 This field shows the number of steps that the diluter performs for aspirating the volume related
1 to the value in the field Relative Position (µL). This visualized value is not modifiable. The
control is directly related to one of the following Check Buttons: Home Position Gap,
Reagent-Sample Gap and Final Gap.
Relative Position (µL).
6 This field shows the volume in microliters to be aspirated/dispensed by the diluter. The Relative
1 Position (µStep) field shows the steps correspondent to the volume. It is possible to delete
this value and introduce it by keyboard. The value must be included between 0 and 500µL, it is
not possible to introduce negative values. Steps to microliters conversion is performed by the
software. The control is directly related to one of the following Check Buttons: Home Position
Gap, Reagent-Sample Gap and Final Gap.
Home Position Gap.
7
When this Check Button is selected, the fields Relative Position (µL) shows the volume (in
1
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microliters) that the ARM1 Diluter must aspirate as probe FIRST AIR GAP after any ARM1 Reset
or washing operation (Y-Home positioning). This gap is the separation between the systemic
solution column and fluid to be sampled when running. The recommended value is 40μL.
Selection of this button disables the others. Set and memorize soon after modification, before
leaving this selection.
In the field Relative Position (µL) the volume in microliters is shown.
Reagent-Sample Gap.
8
When this Check Button is selected, the fields Relative Position (µL) shows the volume (in
1
microliters) that the ARM1 Diluter has to aspirate as probe SECOND AIR GAP during operation
(the gap after reagent aspiration). The suggested value is actually 0μL. Selection of this button
disables the others. Set and memorize soon after modification, before leaving this selection.
Special settings are possible and it can be subjected to modifications.
Final Gap.
9 Actually set to 0. When this Check Button is selected, the fields Relative Position (µL) shows
1
the volume (in microliters) that the ARM1 Diluter must aspirate as probe THIRD AIR GAP during
operation. This gap is generally the one after aspiration of sample (if set). The suggested value
is actually 0μL. Selection of this button disables the others. Set and memorize soon after
modification, before leaving this selection.
Special settings are possible and it can be subjected to modifications.
10
Memorize Gap.
This key allows memorization of the value Gaps just modified. Click on this key ANY TIME you
want to memorize ANY SINGLE gap value and before to go to set another gap. Activation of
this key allows then the memorization of the gap value currently displayed in the field.
WARNING:
11 <Add<.
This command is a facility for some testing operation. It adds volumes selected in the Relative
Position (µL) field to Absolute Position (µL) field.
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1.3.4.2. Samples and Reagents Panel
The Samples and Reagents Panel is composed of three sections: Reagents Wheel, Samples Wheel,
and Bar Code Commands. Each of them allows controlling of a specific part of the instrument.
Reagents Wheel: Reagent Wheel motion.
Samples Wheel: Sample Wheel motion.
Bar Code Commands: Bar Code related commands.
1 2 3
1 1 1
4 6
5
1 1
1
7 8 9
1 1 1
Reagent No.
Introduce the reagent position number into this field and the Position field will automatically
1
show the number of motor steps associated to that position. The value will ranges from 1 to the
1
highest reagent position number on the tray: don’t introduce negative values.
R1 (Ext).
2
Not Used. This Check Button is not active: it remains always selected.
1
Position.
This field shows the number of motor steps, stored into the instrument or in software memory,
3 that are associated to the reagent position, selected in Reagent No., when under ARM1 or
1 ARM2 (if Double Arm version). It shows the number of steps that Reagent Wheel must perform
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in order to move the reagent to the position chosen with the Check Button below it. It is
possible to modify this value, but never introduce negative value.
When Calibrating: calibrate always position nr.1 for all selections (Check Buttons); its value
must be included in the range 0-1600µsteps.
Go To Position.
Click this key to move the Reagent position, selected in the field Reagent No, to the position
4 specified with the Check Buttons: Arm1, Arm2 or Bar Code Reader. The number of motor
1 steps are those shown in the position control field Position.
Memorize.
This key allows memorization of the value in the field Position correspondent to the reagent
position shown in the field Reagent No. and selected by the Check Button. In case of
modification, the software automatically resets all reagent positions (in microsteps) preserving
the fixed pitch.
5
1
WARNING:
Home.
6 Click this key to send the Reagent Wheel in Home Position.
1
Arm1.
This Check Button, when selected, enables in the field Position the visualization of the
microsteps related to the reagent position shown in the field Reagent No, when under the
7 ARM1. Selection of this button disables the others.
By clicking the command Go To Position the reagent wheel will move the reagent position
indicated in Reagent No. field under the ARM1.
Arm2.
microsteps related to the reagent position shown in the field Reagent No, when under the
8 ARM2. Selection of this button disables the others.
1 By clicking the command Go To Position the reagent wheel will move the reagent position
indicated in Reagent No. field under the ARM2.
Bar Code Reader.
reagent “empty” position or position “zero” (ignoring the field Reagent No) when in front of Bar
9 Code Reader. Selection of this button disables the others.
1 By clicking the command Go To Position the reagent wheel will move the reagent position
“zero” in front of Bar Code Reader.
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1
1 2
1
3
1
4 5 6
1 1 1
7 8 9
1 1 1
Sample No.
1 Introduce the sample position number into this field and the Position field will automatically
1 show the number of motor steps associated to that position. This value ranges from 1 to the
highest sample position number on the tray: don’t introduce negative values.
Position.
2 This field shows the number of motor steps, stored into the instrument or in software memory,
1 that are associated to the sample position, selected in Sample No., when under the ARM1,
ARM2 or for Bar Code reading (related to pos.1). It shows the number of steps that Sample
Wheel must perform in order to move the sample to the position chosen with the Check
Button below it. It is possible to modify this value, but never introduce negative value.
When Calibrating: calibrate always position nr.1 for all selections (Check Buttons); its value
must be included in the range 0-1600µsteps.
Smp.
3
This Check Button is not active: it is always selected.
1
Go To Position.
4
Click this key to move the active Sample position, selected in the field Sample No, to the
1
position specified with the Check Buttons: Arm1, Arm2 or Bar Code Reader. The number of
Memorize.
5 This key allows memorization of the value in the field Position correspondent to the sample
1 position shown in the field Sample No. and selected by the Check Button. In case of
modification, the software automatically resets the sample section positions (in microsteps)
preserving the fixed pitch (in the ambit of the same section).
WARNING:
Home.
6
Click this key to send the Sample Wheel in Home Position.
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Arm1.
7
microsteps related to the sample position shown in the field Sample No, when under ARM1.
Selection of this button disables the others.
By clicking the command Go To Position the sample wheel will move the sample position
indicated in Sample No field under ARM1.
Arm2.
8
microsteps related to the sample position shown in the field Sample No, when under ARM2.
By clicking the command Go To Position the sample wheel will move the sample position
indicated in Sample No field under ARM2.
Bar Code Reader.
9
sample “empty” position or position “zero” (ignoring the field Reagent No) when in front of Bar
Code Reader. Selection of this button disables the others.
By clicking the command Go To Position the reagent wheel will move the sample position
“zero” in front of Bar Code Reader.
1
1 2 3 4 5 6
1 1 1 1 1
Note: Use this group of commands only if the instrument has been enabled and equipped with
Barcode Reader.
Read Bar Code.
1
This command allows the Barcode to one-shot reading. In case that no reagent bottles or
1
sample tubes are in front of the Bar Code reader, it returns the text “ **”. The Bar Code value
read is shown in the text field aside.
2 Read Spd.
1 This text field is not affecting normal operation. It regulates the tray rotational speed during
reading: increasing numbers from to 6 gives slower speed. This setting applies only to this panel
and could be useful in case of debugging or failure analysis. Leave it to 0 for normal
operation.
Reagents.
3
This command runs the scanning of all reagent positions. In case that some positions are not
1
correctly decoded it repeats two more times the same operation at lower reagent wheel
speed.
View.
4
This command shows the result of the reagent wheel Bar Code readings. It open one text
1
windows showing association between reagent position and read value (bar code on the
label).
Samples.
5
This command runs the scanning of all sample primary tube positions. In case that some
1
positions are not correctly decoded it repeats two more times the same operation at lower
sample wheel speed.
View.
6
This command shows the result of the sample wheel Bar Code readings. It open one text
1
windows showing association between sample position and read value (bar code on the
label).
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1.3.4.3. ARM2 Position Panel
Arm2 Position Panel includes 3 sections: X positions, Y position, and D position. Each of them
refers to a single motion of the ARM2.
The same functions, rules, indications and considerations given for ARM1 Position Panel are valid and
can be applied to this ARM2 Position panel: refer to ARM1 position panel.
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1.3.4.4. Temperature Control Panel
3
1
1 2 4
1 1 1
5
1
6
1 7
9
The Temperature Control Panel is composed by four sections: Internal temperature, Incubation
Cuvette Temperature, Dilution Cups, Warm Up. Each of them refers to a specific part of the
instrument.
Internal Temperature.
1 This field is no longer used – ignore it.
Warming Up Count Down.
2 This field shows the left time to end (minutes and seconds) during lamp Warming Up [TW (min)
left]. This field cannot be modified by the operator, it’s just a monitor.
Bar Code.
This LED can be selected allows the Bar Code configuration into the instrument. When in ON
status, Bar Code is enabled; when in OFF status Bar Code is not enabled.
3
Bar Code NOT enabled.
Bar Code ENABLED.

ISE.
This LED can be selected allows the ISE Module configuration into the instrument. When in ON
status, ISE Module is enabled; when in OFF status ISE Module is not enabled.
4
ISE Module NOT enabled.
Modulo ISE ENABLED.
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Incubation Cuvette Temperature.

This section shows the Incubation Temperature measured in the bulk of cuvette assembly and
related parameters.
A
E F
D
B G H
I
C
Display Cuvette Temperature.

A
This field shows the Incubation Temperature. This field cannot be modified by the
operator. Temperature is shown in degree Celsius.
Start Heater.
B
This LED shows the status of the incubation Heater Strip powering.
Incubation Heater Strip OFF.
Incubation Heater Strip ON.

Enable Temp. Control.
C
This LED shows whether the system is controlling incubation temperature driving the
Heater Strip. Leave this LED always ENABLED.
5
Incubation temperature control DISABLED.
Incubation temperature control ENABLED.

Temperature Value.
D This field shows the incubation temperature to be reached and regulated by the
instrument (target temperature). It can be modified by keyboard but any modification
is superseded by the User Interface set value. It is suggested not to change the value
shown.
The target temperature is regulated within ±0.1°C (on the point of measurement). The
range to determine alarm conditions is ±1°C around the target temperature; note that
this range is not the real range of variation of fluid temperature into the reading
cuvettes, it is much narrower.
Tolerance level Low.
E This field shows the regulation low limit range for incubation temperature. When the
incubation temperature decreases below this value, the Heater Strip is turned ON
(Enable Temp. Control. LED is ON). This value can be modified by keyboard.
The typical tolerance range for the target temperature is ±0.1°C. It is then suggested to
maintain this value 0.1°C below the target temperature value (Temperature Value
field).
Tolerance level High.
F
This field shows the regulation high limit range for incubation temperature. When the
incubation temperature increases over this value, the Heater Strip is turned OFF (Enable
Temp. Control. LED is OFF). This value can be modified by keyboard.
The typical tolerance range for the target temperature is ±0.1°C. It is then suggested to
maintain this value 0.1°C over the target temperature value (Temperature Value field).
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Alarm level Low.

G This field shows the alarm low limit range for incubation temperature. When the
incubation temperature decreases below this value, the software generates an alarm
(Incubation temperature out of range). This value can be modified by keyboard.
The typical tolerance range for the target temperature is ±1°C. It is then suggested to
maintain this value 1°C below the target temperature value (Temperature Value field).
Alarm level High.
H
This field shows the alarm high limit range for incubation temperature. When the
incubation temperature increases over this value, the software generates an alarm
(Incubation temperature out of range). This value can be modified by keyboard.
The typical tolerance range for the target temperature is ±1°C. It is suggested to
maintain this value 1°C over the target temperature value (Temperature Value field).
Set Temperature Values.
I
After eventual modifications, this key allows memorization of the new values related to
temperature and described above. These values are stored by the software.
Dilution Cups.
This section allows the setting of volumes to be used when sample dilutions or
standard/calibrators dilution curves are created.
A C
Q.ty Dil Sample.

A This field shows the total volume (in microliters) to be dispensed in the cuvette for any
sample dilution operation (pre- and post-dilutions). This volume is the sum of diluent
volume and sample volume (respecting the given dilution ratio). This volume can be
modified by keyboard.
6 The value of 400µL is the typical volume to be used for Clinical Chemistry. Remember
that cuvette dead volume is fixed to 100µL.
Q.ty Dil Standard.

B This field shows the total volume (in microliters) to be dispensed in the cuvette for any
standard/calibrator single dilution operation. This volume is the sum of diluent volume
and standard volume (respecting the ratio required for Calibration Curves
construction). This value can be modified by keyboard.
The value of 200µL is the typical volume to be used (especially for methods that are
requiring highest sample volume and that are run in triplicate). Remember that cuvette
dead volume is fixed to 100µL.
Save Dil Q.ty

C
dilutions and described above. These values are stored by the software.
Warm Up.
This frame gives the possibility to set Warming Up times (in minutes) performed by the
7
instrument after power up.
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A C
B D
TL1 (min).
A
This fixes shows the maximum OFF time (in minutes) over which the instrument executes
Warming Up.
In the picture, if the instrument is turned OFF for less the about 2 minutes the Warming
Up time for lamp is not run at the power up. Typical value for this field is 4 minutes.
TL2 (min).
B
This field fixes the maximum OFF time (in minutes) over which the instrument executes
Warming Up, cuvette washing and Autozero procedure.
In the case above (that’s the recommended one):
 If the instrument is turned OFF for more than about TL1 (min) minutes and for less
than about TL2 (min) minutes the Warming Up time for lamp is run at the power
up (the warming up duration time is specified by the TW (min) field).
 If the instrument is turned OFF for more than about TL2 (min) minutes the
Warming Up time for lamp, refilling and Autozero (Start-up) procedure is run at
the power up (the warming up duration time is specified by the TW field); the
Autozero procedure is launched at the end of the Warming Up time.
TW (min).
C
This field shows the Warming Up time (in minutes) for lamp stabilization. It is
recommended to set = 20 min. Do not set time shorter than 20 minutes.
Save Time.
D
Warming Up and described above. These values are stored by the software.
Start Buzzer.
8 This command turns ON the buzzer (beeper) of the unit. It has beeping test purpose only.
Stop Buzzer.
9 This command turns OFF the buzzer (beeper) of the unit. It has beeping test purpose only.
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1.3.4.5. Reading Test Panel
4 6
Reading Test Panel.

This panel allows calibration and verification of the optical group functionality. It is possible to run
single shot or continues readings by selecting different filters and gains. It is also possible to view the
gains obtained by the calibration cycle.
Read Data Graph.

This section includes a display able to plot reading values vs. time. The results of reading
operation performed by the optical group can be shown on graph.
X-axis shows the time of readings; Y-axis shows the scale of digital readings (16-bit analog-to-
1 digital converter output  0÷65535 counts range max).
The default limit values of the graph are 0 and 65535 counts. The field in the Range section
gives the ability to modify this range in order to magnify particular intervals for fine checking
and tuning operations. Resolution is 1-count.
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Range.
This section permits modification of the Y scale by introducing new limit values for the Y-axis.
Up Limit.
A
This field shows the upper value of the Y-axis scale. This value can be modified by
keyboard. Values can range from 0 to 65535. Negative values are not allowed.
Note: Do not press Enter key on keyboard after modification of this value; to accept the
new value just click on the button New Range.
Down Limit.
B This field shows the lower value of the Y-axis scale. This value can be modified by
keyboard. Values can range from 0 to 65535. Negative values are not allowed.
Note: Do not press Enter key on keyboard after modification of this value; to accept the
new value just click on the button New Range.
New Range.
C
Clicking on this button the new values Up Limit e Down Limit are accepted.
The graph will change its scale and the old values are lost.
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Clear.
D
Clicking on this button to clear the graph. Old values are lost.
Real Time Value.

This section shows the readings and, by setting the “transparent value” it also computes the
Absorbance (O.D.).
A B E
C D
Real Time Value.

A
This field shows the actual reading value. In continuous reading mode this value
changes in order to show the last reading (about 1 per second). Old values are not
stored. This value cannot be modified.
3
Transparent.
B
In this field can be introduced the transparent value related to the cuvette in reading. If
this value is present, the Absorbance is shown (calculation based on real optical path of
0.6cm and normalized to 1cm). It is not necessary to press Enter to store the value.
Optical Path.
C
This field shows the optical path length in cm. The instrument optical path is 0.6000cm.
Do not modify this value. Negative values are not allowed.
O.D. button
D
When pressing this button the O.D. value corresponding to the measured digital value is
updated and shown in the O.D. field.
O.D. field
E
This field shows the Absorbance value refreshed every time the button O.D. is pressed.
This field cannot be modified manually.
Read.
This section allows the Gain setting for each filter, the reading mode setting for manual
monitoring and the visualization of the stored wavelength gains.
D A
B
C
4
E
F G
Single Value.
A
When this Check Button is enabled, the reading is shown as one shot reading without
any digital filtering and conditioning. It’s a raw single reading.
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Packet.
B
When this Check Button is enabled, the reading is shown as the average of 50
consecutive readings with digital filtering and conditioning. It’s the result obtained from
a packet of single readings.
Continuous.
C When this Check Button is enabled, readings are run continuously (one every 1sec
about) in packed mode since the Start button is clicked. To stop continuous readings just
deselect the check button Continuous.
When this Check Button is disabled, readings are run continuously (one every 1sec
about) in packed mode: one reading every time Start button is clicked.
Start.
D
Readings can be run by clicking this button.
In case Continuous has been selected, readings are continuous at 1sec rate
(independently from Single Value or Packet selection).
In case Continuous has not been selected, one shot value is shown (single raw reading
or packet).
Gain Value (grey filed).
E
This field shows the Gain value actually adopted on the reading chain on the automatic
gain amplifier. This field cannot be modified.
Readings are run with the gain value displayed into this field (not in the field below).
Gain Value (white field).
F
This field shows the value for each filter that has been selected. It is anyway possible to
change it by keyboard for testing purposes. Values can range from 0 to 255. The
following cross-reference table shows the relation between the digital value (value in bit
set on the digital potentiometer) and the real gain values.
Bit G Bit G Bit G Bit G Bit G

0 0,15 52 3,13 103 6,05 154 8,98 205 11,90
1 0,20 53 3,19 104 6,11 155 9,04 206 11,96
2 0,26 54 3,24 105 6,17 156 9,09 207 12,02
3 0,32 55 3,30 106 6,23 157 9,15 208 12,08
4 0,38 56 3,36 107 6,28 158 9,21 209 12,13
5 0,43 57 3,42 108 6,34 159 9,27 210 12,19
6 0,49 58 3,47 109 6,40 160 9,32 211 12,25
7 0,55 59 3,53 110 6,46 161 9,38 212 12,31
8 0,61 60 3,59 111 6,51 162 9,44 213 12,36
9 0,66 61 3,65 112 6,57 163 9,50 214 12,42
10 0,72 62 3,70 113 6,63 164 9,55 215 12,48
11 0,78 63 3,76 114 6,69 165 9,61 216 12,54
12 0,84 64 3,82 115 6,74 166 9,67 217 12,59
13 0,89 65 3,88 116 6,80 167 9,73 218 12,65
14 0,95 66 3,93 117 6,86 168 9,78 219 12,71
15 1,01 67 3,99 118 6,91 169 9,84 220 12,76
16 1,06 68 4,05 119 6,97 170 9,90 221 12,82
17 1,12 69 4,10 120 7,03 171 9,95 222 12,88
18 1,18 70 4,16 121 7,09 172 10,01 223 12,94
19 1,24 71 4,22 122 7,14 173 10,07 224 12,99
20 1,29 72 4,28 123 7,20 174 10,13 225 13,05
21 1,35 73 4,33 124 7,26 175 10,18 226 13,11
22 1,41 74 4,39 125 7,32 176 10,24 227 13,17
23 1,47 75 4,45 126 7,37 177 10,30 228 13,22
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24 1,52 76 4,51 127 7,43 178 10,36 229 13,28

25 1,58 77 4,56 128 7,49 179 10,41 230 13,34
26 1,64 78 4,62 129 7,55 180 10,47 231 13,40
27 1,70 79 4,68 130 7,60 181 10,53 232 13,45
28 1,75 80 4,74 131 7,66 182 10,59 233 13,51
29 1,81 81 4,79 132 7,72 183 10,64 234 13,57
30 1,87 82 4,85 133 7,78 184 10,70 235 13,63
31 1,93 83 4,91 134 7,83 185 10,76 236 13,68
32 1,98 84 4,96 135 7,89 186 10,81 237 13,74
33 2,04 85 5,02 136 7,95 187 10,87 238 13,80
34 2,10 86 5,08 137 8,00 188 10,93 239 13,85
35 2,15 87 5,14 138 8,06 189 10,99 240 13,91
36 2,21 88 5,19 139 8,12 190 11,04 241 13,97
37 2,27 89 5,25 140 8,18 191 11,10 242 14,03
38 2,33 90 5,31 141 8,23 192 11,16 243 14,08
39 2,38 91 5,37 142 8,29 193 11,22 244 14,14
40 2,44 92 5,42 143 8,35 194 11,27 245 14,20
41 2,50 93 5,48 144 8,41 195 11,33 246 14,26
42 2,56 94 5,54 145 8,46 196 11,39 247 14,31
43 2,61 95 5,60 146 8,52 197 11,45 248 14,37
44 2,67 96 5,65 147 8,58 198 11,50 249 14,43
45 2,73 97 5,71 148 8,64 199 11,56 250 14,49
46 2,79 98 5,77 149 8,69 200 11,62 251 14,54
47 2,84 99 5,83 150 8,75 201 11,68 252 14,60
48 2,90 100 5,88 151 8,81 202 11,73 253 14,66
49 2,96 101 5,94 152 8,86 203 11,79 254 14,71
50 3,01 102 6,00 153 8,92 204 11,85 255 14,77
51 3,07
Change Gains.
G
By clicking this button the value shown in the Gain Value (white field) is set in the
Gain Value (grey field) and it becomes the actual gain adopted in the reading
chain on the automatic gain amplifier.
View Gains.
H
This button opens a window showing a table with the Gains associated to each filter.
The column Gain lists the Gains resulting from last Calibration cycle.
The column Factory lists the values that have been saved at factory or by the service.
Storing of Gains is possible by activation of Save Gain command: when Save Gain is
clicked values in Gain column are stored and saved in memory. Just close and re-open
this window to see new saved Gains.
Gains under the column Factory are used to detect filter decaying (that means change
in filter gain: if a filter is aging the correspondent gain will vary; the software checks this
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variation and generates alarm in case the gain is higher than the double of the value
previously saved).
A B
C
D E
Filter Position.
A
Select from this menu the filter (wavelength) to be set in the reading chain (for testing or
calibration purposes); the software will show in the field Position the number of motor
steps associated to the filter position (Filter Wheel). The selected filter (wavelength) will
move when Go To Position command is activated.
Note that the pitch between filters is equal to 160 microsteps.
At any filter selection the Gain associated is shown in the field Gain Value (white
field).
WARNING:
FILTER CALIBRATION REQUIRES SERVICE TRAINING ADVANCED SESSION. SEE THE SERVICE
MANUAL FOR THE CORRECT PROCEDURE.
Position.
B
This field shows the number of motor steps, stored into the instrument or in software
5 memory, that are associated to the filter selected in Filter Position. It shows the
number of steps that Filter Wheel must perform in order to move the selected filter on the
optical path. It is possible to modify this value, but never introduce negative value.
When Calibrating  calibrate any single position; values must be included in the range
0-1600µsteps.
Home.
C Click this key to send Filter Wheel in Home Position.
Go To Position.
D
Click this key to move the Filter Wheel so that the selected filter (Filter Position) is send on
the optical path. The number of motor steps are those shown in the position control field
Position.
Memorize.
E
This key allows memorization of the value in the field Position correspondent to the
selected filter.
WARNING:
ACTIVATING THIS COMMAND, THE INSTRUMENT TEMPORARY SAVES MODIFIED
PARAMETERS IN THE PC MEMORY; PARAMETERS MUST BE DOWNLOADED TO THE
INSTRUMENT TO BE OPERATIVE.
IF MODIFIED PARAMETERS ARE NOT DOWNLOADED INTO THE INSTRUMENT, THEY WILL BE
LOST AT BCSERVER PROGRAM SHUT DOWN-AND THE OLD PARAMETERS ARE NOT MODIFIED.
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Cuvette Wheel.
This section shows the cuvette position when on the optical path (reading position).
B
A
C
D E
Cuvette No.
A This field shows the cuvette number in the reading position.
Introduce the cuvette number into this field and the Position field will automatically
show the number of motor steps associated to that position. This value ranges from 0 to
80: don’t introduce negative values.
Position.
B This field shows the number of motor steps, stored into the instrument or in software
memory, that are associated to the cuvette number introduced in the field Cuvette No.
It shows the number of steps that cuvette wheel must perform in order to position the
specified cuvette in reading position (on optical path). It is possible to modify this value.
6 Negative values are not allowed.
When Calibrating: calibrate always position nr.1; values must be included in the range 0-
3200µsteps (check then position 32 to be in the center of optical path).
Home.
C
Click this key to send Cuvette Wheel in Home Position.
Go To Position.
D Click this key to move the Cuvette Wheel so that the cuvette in Cuvette No. is send on
the optical path (reading position). The number of motor steps are those shown in the
position control field Position.
Memorize.
E
This key allows memorization of the value in the field Position correspondent to the
selected cuvette.
WARNING:
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1.3.4.6. Washing Station Panel
2
1
Washing Station Panel.

This panel allows the verification and Calibration of Washing Station assembly functionality. It is also
used to calibrate Cuvette wheel under Washing Station needles and tip.
Cuvettes Wheel.
This section allows the Cuvette Wheel positioning under instrument Washing Station.
B C
A
D E F
Cuvette No.
A This field shows the cuvette number under the first couple of needle of the washing
station.
Introduce the cuvette number into this field and the Position field will automatically
show the number of motor steps associated to that position. This value ranges from 0 to
80: don’t introduce negative values.
Position.
B
memory, that are associated to the cuvette number introduced in the field Cuvette No.
It shows the number of steps that cuvette wheel must perform in order to position the
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specified cuvette under the first couple of needle of the washing station. It is possible to
modify this value. Negative values are not allowed.
When Calibrating: calibrate always position nr.1; during calibration the whit tip of the
washing station (8th position) must stay in the center of the cuvette. Values must be
included in the range 0-3200µsteps.
Home.
C Click this key to move the Cuvette Wheel in Home Position.
Go To Position.
D Click this key to move the Cuvette Wheel so that the cuvette in Cuvette No. is send
under the first couple of needle of the washing station (washing position). The number of
Memorize.
E This key allows memorization of the value in the field Position correspondent to the
selected cuvette.
WARNING:
LOST AT BCSERVER PROGRAM SHUT DOWN-AND THE OLD PARAMETERS IS NOT MODIFIED.
Reset.
F
By clicking this button the Read&Wash assembly performs a complete reset in the
following order: Washing Station, Filter Wheel, Cuvette Wheel.
Y position.
This section allows Y-axis washing station positioning, testing and calibration.
A C D
B
2
Go To Position.
A
Click this key to move the Washing Station so that the washing station head is send to
the position shown in the control field Position.
Cuvette’s button.
B This Check button, when checked, shows in the field Position the number of steps that
the washing station head must perform in order to reach the bottom of cuvette. It is
possible to modify this value. Negative values are not allowed.
Position.
C This field shows the number of motor steps, stored into the instrument or in software
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memory, that are associated to the bottom of cuvette. It shows the number of steps that
the washing station head (needles’ support) must perform to reach the very bottom of
cuvettes during washing and aspiration procedure. It is possible to modify this value.
Negative values are not allowed.
When Calibrating: the tip and the needles (with springs) must touch the internal bottom
of cuvette and slightly slide up.
Home.
D
Click this key to send Cuvette Wheel in Home Position (higher optocoupler sensor).
Memorize.
E This key allows memorization of the value in the field Position correspondent to the
washing station bottom position.
WARNING:
Exempt Pumps / Aspiration Pumps.
These two frames allow the check of the aspiration and dispensing pumps and calibration of
activation times.
B
C
E F G
3 Needle 1 Cuvette 1 ... Needle 6 Cuvette 6.

A Click on the LED to run the correspondent dispensing pump. To disable it just click again
on it. The command Needle 6 Cuvette 6 is not used in this system – ignore it.
Dispensing pump OFF.
Dispensing pump ON.

% Time.
B This field fixes the speed (duty cycle) for all dispensing peristaltic pumps. Values are
included in the range 0-100. The value 100% means: max speed and it is the typical
value.
In case of need it is possible to modify this value, even if it has been calibrated at
factory, to avoid cuvette overflow but at the same time assuring enough volume for
cuvette washing (450uL about). Negative values are not allowed. Typical value for this
instrument is 35% with Welco WPM peristaltic pumps. Lower value need longer First
Dispensing Time but avoid splashes into the cuvette.
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Introduce values by keyboard. Click on Memorize to store the new value.

First Dispensing Time ... Sec./10.
C
This field fixes the time for the peristaltic pumps dispensing period during washing cycle.
Values are specified in 1/10 of second. If needed it is possible to modify this value even if
it has been calibrated at factory and it is strongly suggested not to change it. Negative
values are not allowed. Typical value for this instrument is 7 (=0.7sec) when has been set
30% Time.
The combination of the %Time and First Dispensing Time must allow cuvette washing for
about 450-500µL.
Memorize.
E
This key allows memorization of the values in the field specified above.
WARNING:
Aspiration Pump 1-7.
F
Click on the LED to run and check the aspiration pumps connected to the aspiration
needles. To disable it just click again on it. It enables both the double head diaphragm
pump connected to the washing station 2nd-5th aspiration needles and the peristaltic
pump connected to the washing station 1st aspiration needle.
Tip Aspiration pump OFF.
Tip Aspiration pump ON.

%.
G This field fixes the duty cycle for the aspiration pump. Values are included in the range 0-
100. The value 100% means: max speed and it is the typical value.
If needed it is possible to modify this value even if it has been calibrated at factory; it is
anyhow recommended to set 100 (max pump power). Negative values are not allowed.
Suction Tip.
H Click on the LED to run and check the diaphragm aspiration pump connected to the
washing station white tip. To disable it just click again on it.
Tip Aspiration pump OFF.
Tip Aspiration pump ON.
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Cycle Control Panel
5 4 6
1
2 3
8 10
7
11
9
12
Cycle Control Panel.

Commands and controls included in this panel allow refill, reading and washing cycles for all reading
cuvettes (without the need to open the BCClient – User Interface) and cycle control parameters
setting. Also reading limits and threshold can be set.
Refill.
Click this button when a Refill cycle must be run (washing station and probe tubing refilling).
1 This cycle includes a given number of dispensing shot cycle for the 5 dispensing washing
station needles in position 1 to 5.
Nr. of 1-to-5 needles refilling shots in the refilling Cycle.
This field (1st on the right) sets the number of dispensing cycle for the 5 dispensing washing
2 station needles in position 1 to 5. Set it to 45 (this number is set in the 1st field on the right and it
should set at 30 – enough for filling tubes starting from empty condition).
Introduce values by keyboard. Click on Save to store the new value.
Nr. of 6th needle no longer used.
This field (2nd on the right) is no longer used. Set it to 1 to gain time.
3
Start Read Cycle.

Click this button when an Autozero (Read and Wash) cycle must be run. This cycle includes
4 washing of all cuvettes and read of “autozero” at all wavelength (transparent value read on
cuvette filled with systemic solution for all filters). New transparent values for all cuvettes are
stored in memory. It doesn’t include tubes refilling.
Start Wash Cycle.
Clicking this button when a pure Washing cycle must be run. The cycle includes only washing
5
of all cuvettes; no reading (autozero) is performed. It doesn’t include tubes refilling.
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Start Read Cycle (Calibration).

Click this button when a Filter Gain Calibration cycle must be run. This cycle is composed by
two phases:
 The first phase includes washing of all cuvettes and read of “autozero” at 340nm
wavelength (transparent value read on cuvette filled with systemic solution) at gain=1
(dig. pot. value=15). At the end of this phase the cuvette with higher value is taken into
consideration and the ratio between filters is calculated on it, in order to equalize filter
6
gains to reach the target value for transparent (zero). The target value is set in the field
ABS. for 340. The calculated gains are then stored in memory by the software and
they will be used for further reading operations.
 The second phase includes a complete Autozero cycle at all wavelength in order to
store in the instrument memory the real “zero values” for all cuvettes and for all filters,
each of them read with the real stored filter gain.
It doesn’t include tubes refilling.
Save.
7 Click on this button to store in memory new values introduced in these fields: refill shots, ABS.
for 340, %OK, Gain percent, Min abs Lamp.
ABS. for 340.
This field shows the target value to be reached during calibration; it fixes the dynamic range.
If needed it is possible to modify this value even if it has been fixed at factory for best
8 performance. Negative values are not allowed. Values ranges from 0 to 65535: typical value is
20000 that gives signal-to-noise ratio optimization (never set values below 10000 nor over
60000).
% Absolute Read OK.
This field shows the percentage value that fixes the threshold for cuvette to be marked as dirty.
When the transparent (zero) value read on a cuvette exceeds this percentage of the value
fixed in the field “ABS. for 340”, that cuvette is marked as dirty and it is no more used by the
9 system until it is detected as clean again (cuvette could be damaged or too dirty).
If needed, it is possible to modify this value even if it has been fixed at factory for best
performance. Negative values are not allowed. Values ranges from 0 to 100: typical value is
30%.
Gain percent.
This field shows the gain percentage value that fixes the threshold for filters to generate an
alarm for aged or damaged filter. When the transparent (zero) value read during calibration
on certain filter gives a gain such that the gain stored at factory is less than the given
10 percentage of the new value (that means too high gain), an alarm is generated.
50%.
Min abs Lamp.
This field shows the value for readings below which an alarm is generated: if more than 10
consecutive readings give a value below that one, the lamp is detected as burned.
11 If needed, it is possible to modify this value even if it has been fixed at factory for best
50 (never go over 300).
% Previous Read OK.
12 This field shows the percentage value that fixes the threshold for cuvette to be marked as
dirty. When the transparent (zero) value read on a cuvette exceeds this percentage of the
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previous reading for the same cuvette, that cuvette’s zero is marked as non valid and the
cuvette is no more used by the system until it is detected a consistent and valid value.
performance. Negative values are not allowed. Values ranges from 0 to 100: typical values
are 5% or 10%.
A “Gain Calibration cycle” resets this situation by deleting older cuvette reading memory.
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1.3.4.7. Cycle Timings Panel

This panel is not used: it is not described in this document because it doesn’t have any utility for
testing, checking or calibrating the instrument.
1.3.4.8. Cycle Status Panel

This panel is not used: it is not described in this document because it doesn’t have any utility for
testing, checking or calibrating the instrument.
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1.3.4.9. Status Panel
1 2
8
3 4
5 6
9 10
11 12
Status Panel.
This panel allows monitoring of the instrument and of the Controller boards status; current status is
visualized. Windows related to software and hardware warnings and alarms can also be shown.
Status.
1 This field monitors the instrument server software and the firmware status.
Client Status.
2 This field monitors the User Interface software status.
Master.
This field shows the Main Controller board (here called Master) status. The correct operation is
3
identified by the string “UP”. By clicking the LED aside the software inquires the Main Controller
board in order to update the current status.
RW.
This field shows the R&W Controller board (Read&Wash) status. The correct operation is
4
identified by the string “UP”. By clicking the LED aside the software inquires the R&W Controller
board in order to update the current status.
Arm1.
This field shows the ARM1 Controller board status. The correct operation is identified by the
5
string “UP”. By clicking the LED aside the software inquires the ARM1 Controller board in order
to update the current status.
Arm2.
6 This field shows the ARM2 Controller board status. The correct operation is identified by the
string “UP”. By clicking the LED aside the software inquires the ARM2 Controller board in order
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to update the current status.

Machine status.
This field shows the instrument status. The correct operation is identified by the string
7 “FUNCTIONING”. By clicking the LED aside the software inquires the instrument in order to
update the current status.
Status Stack.
This debug field shows the next status following the current one; when the current status is
8
“ERROR” in this field it is possible to recognize if, resetting the error the instrument goes back to
the “ANALYSIS” (Running) status or in “IDLE” status (Stand by), etc.
Enable SC.
This command enables Smart Card operation. After that this command has been activated,
9 the software asks for a unique password for enabling (request it to the Manufacturer); it is then
needed to shut down the software from BCClient User Interface and to restart it.
Disable SC.
10 This command disables Smart Card operation. After this command has been activated, the
software asks for another unique password for disabling (request it to the Manufacturer); it is
then needed to shut down the software from BCClient User Interface and to restart it.
Warnings.
11 This window visualizes active warnings on the instruments. When the eventual warning turns off
the related message is deleted.
Alarms.
12
This window visualizes active alarms on the instruments. When the eventual warning turns off
the related message is deleted.
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1.3.4.10. ISE Control Panel
1 3 4
ISE Module Control panel.

This panel allows the monitoring, driving and setting of the ISE Module when included in the
instrument.
Ignore this panel if ISE Module option is not included.
A E
B F
1
C G
D H
Verify.
A Command for sending to the ISE Module a verification message (“ISE?”). If the ISE
Module works correctly the Debug Window will show the answer message: “ISE!”.
Version.
B Command for sending to the ISE Module the request for its running software version. If
the ISE Module works correctly the Debug Window will show the ISE Module firmware
version (checksum).
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Rgt 1.
C Command for displaying the first page of data stored into the flash memory of the
reagent pack Dallas chip.
Rgt 2.
D
Command for displaying the second page of data stored into the flash memory of the
reagent pack Dallas chip.
Purge A.
E Command used to Purge Calibrant A into ISE Module inlet port. The cycle consists in
purging Calibrant A from Reagent Pack, in order to fill tubing. Calibrant A is purged
through the related peristaltic pump with a given short quantity. Several purge
commands can be needed and the waste pump is automatically enabled at the end
of each cycle. When the Calibrant A is in front of electrodes the Debug Window will
show the answer message: “ISE!”.
WARNING:
DURING STAND BY, AFTER THE LAST ISE SAMPLE HAS BEEN PROCESSED, THE ISE MODULE
AUTOMATICALLY RINSES CALIBRANT A IN FRONT OF THE ELECTRODES EVERY 30 MINUTES
(SIP CYCLES).
Purge B.
F Command used to Purge Calibrant B into ISE Module inlet port. The cycle consists in
purging Calibrant B from Reagent Pack, in order to fill tubing. Calibrant B is purged
through the related peristaltic pump with a given short quantity. Several purge
commands can be needed and the waste pump is automatically enabled at the end
of each cycle. When the Calibrant A is in front of electrodes the Debug Window will
show the answer message: “ISE!”. After a Purge B run always a Purge A command to
leave Calibrant A in front of the electrodes.
Bubble Cal.
G This command runs the check and the calibration of the bubble detector (that
nevertheless is automatically performed by the instrument at the system start up during
Warming Up). At the end the Debug Window will show the result of the calibration.
Cal.
H Command used for running the ISE Module “electrodes calibration”. The ISE Module will
automatically cycle Calibrant A and Calibrant B in front of the electrodes and provides
mV measurement (this is automatically performed by the instrument at the system start
up during Warming Up cycle). One of these calibrations is required at the beginning of
every day. Calibration is also automatically performed after 8 hours and after any Clean
Cycle. At the end the Debug Window will show the result of the calibration in terms of
mV (two measures).
The millivolt readings coming from the calibration are then used to set up a relationship
between sample concentration and electrode millivolt output. The change in millivolts
per change in concentration is the slope of the electrode. The slope of the electrodes is
reported in mv/dec (millivolts per decade change in concentration), and should be
within the following limits:
Li+ 47-64 mV/dec, Na+ 52-64 mV/dec, K+ 52-64 mV/dec, Cl- 40-55 mV/dec
The ISE Module Calibration Cycle performs two successive calibrations. The slopes must
be repeatable within 1.5 mV/decade change.
Calibration is also automatically performed after 8 hours and after any Clean Cycle.
2 A B C D
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Pump Cal.
A
Command used for calibrating the ISE Module peristaltic pump stepping motors. (That
nevertheless is automatically performed by the instrument at the system start up during
Warming Up). At the end the Debug Window will show the result of the pump calibration
in terms of motor steps. The Pump Calibration values of A and B should be within the
range 1500 to 3000.
Maint.
B
Command recommended before replacing electrode or before to store them. It
enables only the waste pump to empty the electrodes’ fluid path.
WARNING:
THIS COMMAND MUST BE RUN EVERY TIME ELECTRODES MUST BE REMOVED.
DO NOT LEAVE ELECTRODES IN PLACE AFTER RUNNING THIS COMMAND FOR MORE THAN
30 MINUTES. TO RE-ESTABLISH NORMAL WORKING CONDITIONS AFTER A MAINT
COMMAND, RUN “PURGE CALIBRANT A” CYCLES.
Clean.
C
Command used to clean and to remove and prevent protein build-up from electrode
path. This cycle makes use of a special cleaner solution placed on the reagent tray. The
instrument automatically runs a Clean cycle after that 50 samples have been
processed. It is suggested to run anyhow a clean cycle at the end of any working day.
After a Clean cycle the instrument automatically performs the electrode calibration.
Start Up.
D
This command runs an ISE Module initialization command in the following sequence:
Purge A, Purge B, Purge A, Bubble detector calibration, Pump calibration and Electrode
calibration.
E
A C
B D
Urine Analysis Settings Reaction Quantity.

A
This field shows the volume (in µL) of urine sample used for each of the two dispensations
during urine analysis. The quantity of urine that the Sampler ARM1 doses from primary
tube is equal to the double of this volume plus the volume indicated in the following
field Urine Analysis Settings, Needle Exceed Quantity.
performance. Negative values are not allowed. Typical values are 90 (never set this
value below 70).
Urine Analysis Settings Needle Exceed Quantity.
B
This field shows the volume (in µL) of urine sample inhaled in excess during urine analysis
sample aspiration.
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value below 10).
Sample (Serum) Analysis Settings Reaction Quantity.
C
This field shows the total reaction volume (in µL) of serum sample to be dispensed during
serum analysis.
value below 70).
Sample (serum) Analysis Settings Needle Quantity.
D
This field shows the total volume (in µL) of serum sample used for aspiration of the sample
during serum analysis (it includes reaction volume and quantity in excess).
value below 80).
E Save.
Click on this button to store in memory new values introduced in fields above.
4
A F
C G
D
H
E I
Go X.
A
Click this key to move the ARM1 X-Axis on the ISE Module sample cup. The number of
motor steps are those shown in the position control field Position X.
Home (X).
B
Click this key to send the ARM1 in X-axis Home Position.
Down.
C Click this key to move the ARM1 Y-Axis to the ISE Module sample cup protection height.
The number of motor steps are those shown in the position control field Down Position Y.
It is the very bottom co-ordinate into the ISE Module sample cup (protection) used also
for Calibrant aspiration and dispensing during Pump calibration.
Disp.
D
Click this key to move ARM1 Y-Axis on the ISE Module sample cup dispensing height.
The number of motor steps are those shown in the position control field Dispensing
Position Y. It is the coordinate used to enter the ISE Module sample cup when
dispensing samples inside it.
Home Y.
E
Click this key to send ARM1 up to Y-axis Home Position.
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Position X.
F
memory, that are associated to the ARM1 position over ISE Module sample cup. It shows
the number of steps that ARM1 X-axis must perform o reach ISE Module sample entry top
cup. It is possible to modify this value.
Down Position Y.
G
memory, that are associated to the protection co-ordinate into the ISE Module sample
cup. It is the very bottom co-ordinate into the ISE Module sample cup (protection) used
also for Calibrant aspiration and dispensing during Pump calibration. It shows the
number of steps that ARM1 must perform along Y-axis in order to move to about 1/2mm
from the bottom of the sample cup. It is possible to modify this value: never introduce
negative values.
Dispensing Position Y.
H This field shows the number of motor steps, stored into the instrument or in software
memory, that are associated to the dispensing co-ordinate into the ISE Module sample
cup. It shows the number of steps that ARM1 must perform along Y-axis in order to move
to dispensing position into the sample top entry cup. It is possible to modify this value:
never introduce negative values. Typical values are about 25 steps less (higher) than the
coordinate Down Position Y.
Mem ISE Position.
I
This key allows memorization of the positions above.
WARNING:
A B C D
E
F
G
5
Install Date.
A This field shows the Reagent Pack installation date that is automatically assigned by the
software when a new Reagent Pack is installed. This information is contained within
Reagent Pack chip memory. This value cannot be modified.
Expiration Date.
B This field shows the Reagent Pack installation date that is automatically read by the
software into the Reagent Pack. This information is contained within Reagent Pack chip
memory. This value cannot be modified.
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Cal A.
C
This field shows the Calibrant A / B volumes contained into the Reagent Pack. This
approximate value is automatically updated by the software during operation.
Status Window.
D
This field shows the actual status of the ISE Module.
Reset.
E
Command sending a RESET to the ISE Module. It also deletes the list of command stack.
Debug Window.
F
This window shows part of the messages that the instrument exchanges with the ISE
Module.
Empty List.
G
Click on this command to clear all messages into the Debug Window.
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1.3.5. BCServer window
UNTITLED – BCSERVER.
This panel gives the status of all cuvettes by showing the transparent value (zero value measured on
cuvette filled with systemic solution.
This panel also includes commands for parameters download.
Note: By closing this window the BCServer application shuts down. This is the only way to quit
BCServer (apart from BCClient User Interface).
GRAPH.
1
The graph represents cuvette readings during and after the Autozero procedure (Read&Wash
cycle). Each filter reading is shown with a different color.
The X-axis represents the number of the single cuvette (from 1 to 80); the Y-axis represents the
counts of the Analog-to-Digital converter that is part of the optical reading chain.
During Autozero the system plots readings in real time.
Filter Selection.
This menu allows the selection of the filter to be focused. For the selected filter the window
below shows reading values (zeros or transparent values).
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Autozeros (Transparent Values).

This window lists the reading values for each cuvette related to the selected filter.
3
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1.3.6. Parameters Download
1 2
About BCServer.
In order to download calibration parameters into the instrument firmware memory it is necessary to
open the dialog window About BCServer ; it is possible to enter this windows by clicking on
command “Help” on the menu bar from the window BCServer:
Help»About BCServer
Save All Parameters.

This is the command to start parameters download; all parameters that have been modified
can be in this way updated on the instrument. At the end of the process the window will
automatically close in case of good result; if not, repeat all operations.
1
WARNING:
AT THE END OF THIS OPERATION, IT IS NECESSARY TO SHUT DOWN AND RESTART THE INSTRUMENT
AND THE BCSERVER SOFTWARE. IN THIS WAY NEW PARAMETERS ARE RELOADED BY THE
SOFTWARE. IN THE OTHER CASE THE SYSTEM WILL CONTINUE WORKING WITH OLD PARAMETERS.
Load Last.
This is the command to start parameters upload. All parameters that have been saved into a
“special file” on the PC hard-disk by the BCServer software are reloaded by the BCServer
program and are ready to be downloaded. This is very useful in case of parameters corruption
in the instrument (just click on the command Save All Parameters after uploading).
Note that on command Save All Parameters the software replace all parameters on the
2 screen into the “special file” and old one are lost.
WARNING:
BE CAREFULL WHEN USING THIS COMMAND; BE SURE TO USE IT ONLY WHEN OPERATIONS ARE
UNDER CONTROL AND YOU ARE SURE THAT PARAMETERS HAVE BEEN CORRUPTED IN THE MAIN
CONTROLLER FLASH MEMORY: THIS COMMAND OVERWRITE THE ACTUAL FLASH MEMORY
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CONTENT.
Machine Serial Number:

This field shows the Serial Number of the instrument; the Serial Number has been given at
3 factory and it is not possible to modify it manually.
Use MSTool to do it.
OK.
4 This command closes the window About BCServer.
Note on saving / loading characteristic parameters of the instrument.

To be clear just some hints on the way the systems manages parameters.
1- The system stores parameters locally into the flash memory of the Main Controller board.
2- The system loads parameters from the instrument flash memory at the start up and it shows
them on the monitor; it also uses those parameters during operation.
3- After any parameters modification and memorization, the system, on user command “Save
all parameters”, saves new parameters into the special files: content.fls, mConfig.dat,
arm1Config.dat, arm2Config.dat, rwConfig.dat (folder C:\BCA\Comunication\EXE) and at
the same time downloads those into the instrument flash memory.
4- The system, on user command “Load last”, loads new parameters from the special files:
content.fls, mConfig.dat, arm1Config.dat, arm2Config.dat, rwConfig.dat (folder
C:\BCA\Comunication\EXE), it shows them on the monitor and at the same time
downloads those into the instrument flash memory.
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1.4. MSTool – Service SW Tool for Altair Instruments

This tool is provided to distributors of the Altair™ 240 instrument and it helps authorized
service personnel to do common repetitive jobs on the instruments and helps calibration.
MSTool has been designed to work only with software version 2.x and higher.
It has been designed in order to help operators during servicing or recalibrating
instruments and also to do activity like method management (i.e.: reset of analysis
counter, …) and Log management (i.e.: Los files exporting or deleting).
MSTool can be launched in a Personal Computer where Altair™ software is actually

installed but not contemporary running. Then, in order to avoid connection problems and
data corruption, Altair™ Software (BCClient and BCServer) must be previously closed.
MSTool also requires administrator privileges on the computer and a log-in password, set
as adminservice.
Note: Refer to the last valid revision of the MSTool Guide code MNU-10936-01-_ , it gives
information and detailed user instructions about the use of this tool.
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Random Access Analyzer

Service Software Manual

DAR# 16.0403 ROM.G6004.00 EFF Date: 04/19/16

i.1 Identification Data

 Altair™ 240: Ref # G6000-001

i.3 Purpose of This Document

i.4 Use of This Manual

i.5 List of Contents

Section 1 SOFTWARE APPLICATION FOR SERVICE

1.1. Legend for Symbols Adopted in This Section

1.2. Command and Functions - Information

Example Type Name and function

MEMORIZE GAP. It stores in memory the value shown in the

1.3. Client and Server Software

1.3.1. Starting BCClient and BCServer

Operation Name and Function

In normal installations the BCServer starts and works in

During initialization, the User Interface software (BC

User name: admin

And then press <Enter>.

If codes entered are correct,

After initialization, on the bottom right side of the

Operation Name and Function

Then click OK or press Enter.

If user and password are correct, the BCServer,

1.3.2. BCServer Software

1.3.3. Configuration and Control Panels

Client configuration and Control.

It is possible to enter Sensors Test sub-menus by

Master Sensors: Main Controller board

The list of sensors under monitoring related to the

Do not use nor modify the following control panels:

1.3.3.1. Master Sensors – Acquired by the Main Controller board

Reagent tray NOT in Home position.

Sample tray NOT in Home position.

Systemic solution tank NOT empty.

Cleaner solution tank NOT empty.

Waste tank NOT full.

1.3.3.2. ARM1 Sensors – Acquired by the ARM1 Controller board

ARM1 NOT in Home X position.

ARM1 NOT in Home Y position.

ARM1 IN Home Y position.

ARM1 diluter NOT in Home position.

ARM1 diluter IN Home position.

Liquid level NOT detecting.

Liquid level DETECTING a capacitive variation.

Obstacle NOT detected.

1.3.3.3. ARM2 Sensors – Acquired by the ARM2 Controller board

ARM2 NOT in Home X position.

ARM2 NOT in Home Y position.

ARM2 IN Home Y position.

ARM2 diluter NOT in Home position.

ARM2 diluter IN Home position.

Liquid level NOT detecting.

Liquid level DETECTING a capacitive variation.

Obstacle NOT detected.

1.3.3.4. RW Sensors – Acquired by the R&W Controller board

Cuvette wheel NOT in home position.

Filter wheel NOT in home position.

Filter wheel IN home position.

Washing station NOT in Home Position.

Washing station IN Home Position.

Washing Low Sensor.