Yeast expression vectors

Yeast Expression Systems(P pastoris / S cerevisiae)

INQUIRY

Utilizing Profacgen's proprietary YeastXceedTM Technology,

selected stable and durable production strains that resemble

mammalian system are employed for high-yield, high-productivity

protein processing, thus dramatically reducing the associated costs

and inherent time requirements.

HostOptimTM boosts recombinant protein production per cell by

increasing the protein per mRNA ratio across a range of eukaryotic

production hosts. Other available recombinant production

technologies typically target transcription and are limited to specific

production systems. HostOptimTM can be used to increase the

production yield of your recombinant protein and can be combined

with transcription enhancing or other production improving

technologies.

Yeast protein expression system is the most economical eukaryotic

expression system for both secretion and intracellular expression. It

is ideally suitable for large-scale production of recombinant

eukaryotic proteins.

Our expertise in protein expression spans both P. pastoris and S.

cerevisiae expression systems. If you're looking for protein

production via a fed-batch pichia processes, We have the

experienced and credentialed staff to move your project forward.

Our YeastXceedTM Technology considers promoters, optimal

copies of vector per cell, inducibility, signal peptides, and cellular

location, which are essential for high-level recombinant protein

production.

Profacgen's engineering-enhanced protein expression

technologies enable a high success rate of over 95% over a wide

variety of recombinant proteins, and our proprietary YeastXceedTM

technology is pushing yields even higher. Many secretory proteins

that bacterial expression systems yield as insoluble or as inactive

inclusion bodies can be produced successfully by our yeast

expression system using native or yeast secretion signals.

We have identified DNA elements which are instrumental in

increasing translation rates when included in recombinant protein

expression vectors. These elements are functional across

eukaryotic production platforms, including mammalian cells, yeast,

and most fungal systems. Based on these elements, Profacgen has

developed its proprietary HostOptimTM technology.

Using a range of mammalian, yeast and fungal cell types, the

functionality of HostOptimTM technology has been confirmed for a

variety of industry relevant platforms. Depending on initial

expression levels, expression of secreted recombinant proteins can

be increased several-fold, and is coupled to a similar increase in

recombinant protein to mRNA ratios.