Current Research, Technology and Education

Topics in Applied Microbiology

and Microbial Biotechnology

FORMATEX
Microbiology Series Nº 2

VOL. 2

Edited by

A. Méndez-Vilas
Vol. 2
ISBN-13: 978-84-614-6195-0

FORMATEX
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 Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology
A. Méndez-Vilas (Ed.)
_______________________________________________________________________________________

CONTENTS
VOL. 1

Introduction............................................................................................................................. XVII

Environmental Microbiology, Geomicrobiology,

Soil Microbiology, Biocontrol

Waterborne transmission of Cryptosporidium and Giardia: detection, surveillance and

implications for public health
D. Carmena................................................................................................................................ 3-14

Purification of enzymes related to host penetration and pathogenesis from

entomopathogenic fungi
S. Ali, S.X. Ren, Z. Huang and J.H. Wu................................................................................... 15-22

Polyamines in cyanobacteria: biosynthesis, transport and abiotic stress response

Aran Incharoensakdi, Saowarath Jantaro, Wuttinun Raksajit, and Pirkko Mäenpää................ 23-32

Adaptation to oxidative stress by Gram-positive bacteria: the redox sensing system

HbpS-SenS-SenR from Streptomyces reticuli
Matthew R. Groves, and Darío Ortiz de Orué Lucana.............................................................. 33-42

Pioneer communities in the forefields of retreating glaciers: how microbes adapt to a

challenging environment
Lazzaro A, Franchini AG, Brankatschk R, Zeyer J................................................................... 43-52

Molecular Microbial Ecology of Marmara Sea Sediments

O. Ince, M. Kolukirik and B.K. Ince......................................................................................... 53-63

Sludge oomph: harnessing the power of sediment microbiota

Alexandra S. Alves, Bruno M. Fonseca and Ricardo O. Louro................................................ 64-73

Comparative analysis of two phytohormone and siderophores rhizobacteria producers

isolated from heavy metal contaminated soil and their effect on Lens esculenta growth
and tolerance to heavy metals
M.O. Franco-Hernández, S. Montes-Villafàn, M. Ramírez-Melo, A. Rodríguez-Dorantes,
A. Rodriguez- Tovar, A.N. Ruíz-Flores, M.S. Vásquez-Murrieta and
A. Ponce-Mendoza.................................................................................................................... 74-80

Capturing the potential of Haloalkaliphilic bacteria from the saline habitats through
culture dependent and metagenomic approaches
S. P. Singh, M. K. Purohit, V.H. Raval, S. Pandey, V. G. Akbari and C. M. Rawal................ 81-87

Characteristics of Extremophylic Fungi from Chernobyl Nuclear Power Plant

Т. Belozerskaya, K. Aslanidi, А. Ivanova, N. Gessler, A. Egorova, Yu. Karpenko, and S.
Olishevskaya............................................................................................................................. 88-94

Induction of secondary symbiosis between the ciliate Paramecium and the green alga
Chlorella
Y. Kodama, and M. Fujishima.................................................................................................. 95-102

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A. Méndez-Vilas (Ed.)
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Isolation and characterization of bioluminescent bacteria from marine environments

of Puerto Rico
J. Malave-Orengo, Eva N. Rubio-Marrero and C. Rios-Velazquez......................................... 103-108

Isolation and characterization of purple non-sulfur anoxyphototropic bacteria from

two microecosystems: tropical hypersaline microbial mats and bromeliads
phytotelmata
K. Soto-Feliciano, M. De Jesús, J. Vega- Sepúlveda and C. Ríos-Velázquez.......................... 109-116

Characterization of blue pigmented bacteria isolated from Puerto Rico

V. Cardona-Cardona, David Arroyo, J. Scellekens and C. Rios-Velazquez............................. 117-123

Bacterial Extracellular Enzymatic Activity in Globally Changing Aquatic Ecosystems

A. Cunha, A. Almeida, F.J.R.C. Coelho, N. C. M. Gomes, V. Oliveira, and A. L. Santos...... 124-135

Bacteria in molluscs: good and bad guys

J.L. Romalde, and J.L. Barja..................................................................................................... 136-147

Antioxidative defense systems of anaerobic sulfate-reducing microorganisms

A. Brioukhanov, L. Pieulle and A. Dolla.................................................................................. 148-159

Evaluation of sol-gel silica matrices as inoculant carriers for Mesorhizobium spp. cells
G. S. Alvarez, F. L. Pieckenstain, M. F. Desimone, M. J. Estrella, O. A. Ruiz and L. E.
Díaz........................................................................................................................................... 160-167

Biological fertilization
Mohammad Miransari............................................................................................................... 168-176

Metabolic strategies deployed by Pseudomonas fluorescens to combat metal

pollutants: Biotechnological prospects
Joseph Lemire, Christopher Auger, Adam Bignucolo, Varun P. Appanna, and Vasu D.
Appanna..................................................................................................................................... 177-187

Adsorptions of DNA molecules by soils and variable-charged soil constituents

K. Saeki and T. Kunito.............................................................................................................. 188-195

Mechanisms of infection used by Xanthomonas axonopodis pv. citri in citrus canker

disease
N. Gottig, B.S. Garavaglia, C.G. Garofalo, T. Zimaro, G.G. Sgro, F.A. Ficarra, G. Dunger,
L.D. Daurelio, L. Thomas, C. Gehring, E.G. Orellano and J. Ottado....................................... 196-204

Proteomics of fungal plant pathogens: the case of Botrytis cinerea

R. González Fernández, and J.V. Jorrín Novo..................................... .................................... 205-217

The universe of basidiomycetous ground fungi

G. Gramss.................................................................................................................................. 218-229

Management of the Mycorrhizal Soil Infectivity to Improve Reforestation Program

Achievements in Sahelian Ecosystems
Arsene Sanon, Fatou Ndoye, Ezékiel Baudoin, Yves Prin, Antoine Galiana and Robin
Duponnois................................................................................................................................. 230-238

Phytophthora cinnamomi suppressive soils

B. Keen and T. Vancov............................................................................................................. 239-250

IV ©FORMATEX 2010
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Rhizospheric plant-microbe interactions that enhance the remediation of

contaminated soils
E. Amora-Lazcano, L.A. Guerrero-Zúñiga, A. Rodriguez-Tovar, A. Rodriguez-Dorantes
and M.S. Vasquez-Murrieta...................................................................................................... 251-256

Investigating the role of microorganisms in soil seed bank management

J. Chee-Sanford, and X. Fu....................................................................................................... 257-266

On the life of Ralstonia solanacearum, a destructive bacterial plant pathogen

Belén Álvarez, Elena G. Biosca, and María M. López............................................................. 267-279

Molecular diversity and enzymatic potential of salt-tolerant alkaliphilic actinomycetes

S. P. Singh, J.T. Thumar, S.D. Gohel, and M. K. Purohit........................................................ 280-286

Fate and environmental impact of the recently marketed herbicide Mesotrione:

coupling biological and chemical studies for a global overview
Isabelle Batisson, Martine Sancelme, Clarisse Mallet, and Pascale Besse-Hoggan................. 287-294

A challenge to mushroom growers: the green mould disease of cultivated champignons

L. Kredics, L. García Jimenez, S. Naeimi, D. Czifra, P. Urbán, L. Manczinger, C.
Vágvölgyi, and L. Hatvani........................................................................................................ 295-305

Nitrogen metabolism in ectomycorrhizal fungi: fHANT-AC gene regulation in

Laccaria bicolor
Minna J. Kemppainen and Alejandro G. Pardo......................................................................... 306-318

Biological soil quality indicators: a review

Martinez-Salgado M,M., Gutiérrez-Romero, V., Jannsens, M., Ortega-Blu, R........................ 319-328

Fungal weathering and implications in the solubilization of metals from soil and from
asbestos fibres
S. Daghino, E. Martino and S. Perotto...................................................................................... 329-338

Lecanicillium muscarium as microbial insecticide against whitefly and its interaction

with other natural enemies
S.X. Ren, S. Ali, Z. Huang and J.H. Wu................................................................................... 339-348

Novel Fermentation media for the production of mosquito pathogenic bacilli in

mosquito control
Subbiah POOPATHI................................................................................................................. 349-358

Antimicrobials, Susceptibility, Resistance,

Biosynthesis of antibiotics
Inorganic nano mental oxides used as anti-microorganism agents for pathogen control
Lu-E Shi, Liangying Xing, Baochao Hou, Hongjuan Ge, Xiaofeng Guo and Zhenxing
Tang........................................................................................................................................... 361-368

Antimicrobial efficacy and systematic use of disinfectants

K. Steinhauer............................................................................................................................. 369-376

Identification and bioactive properties of endophytic fungi isolated from phyllodes of

Acacia species
H.B.Q. Tran, J.M. McRae, F. Lynch, and E.A. Palombo.......................................................... 377-382

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 Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology
A. Méndez-Vilas (Ed.)
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“Weapons of a pathogen: Proteases and their role in virulence of Pseudomonas

aeruginosa”
R. Hoge, A. Pelzer, F. Rosenau, S. Wilhelm............................................................................. 383-395

Antimicrobial activity of secondary metabolites and lectins from plants

P.M.G. Paiva, F.S. Gomes, T.H. Napoleão, R.A. Sá, M.T.S. Correia and
L.C.B.B. Coelho........................................................................................................................ 396-406

Nanobiotechnology: A new strategy to develop non-toxic antimicrobial textiles

Isabel C. Gouveia...................................................................................................................... 407-414

Pitfalls of antimicrobial susceptibility testing of enterococci isolated from farming

broilers by the disk diffusion method
Manuela Oliveira, Verónica Santos, Amélia Fernandes, Sandro Filipe Nunes, Fernando
Bernardo, and Cristina Lobo Vilela.......................................................................................... 415-418

Multi-functions of photodynamic pigments in ciliated protozoans

T. Matsuoka, H. Kotsuki and Y. Muto...................................................................................... 419-426

Blueberries: Genotype-dependent variation in antioxidant, free-radical scavenging,

and prebiotic activities
A.L. Molan, M. A. Lila and G. Ravindran................................................................................ 427-434

Microbial superoxide dismutase enzyme as therapeutic agent and future gene therapy
Hatem M. El Shafey, Saleh A. Bahashwan, Abdulaziz A. Alghaithy
and Samah Ghanem................................................................................................................... 435-443

Fruit and vegetable peels – strong natural source of antimicrobics

S. Chanda, Baravalia Y, Kaneria M and Rakholiya K.............................................................. 444-450

Novel leads from herbal drugs for infectious skin diseases

S. Chanda and Y. Baravalia....................................................................................................... 451-456

Inhibitory Effect of Green Onion on the Growth of Aspergillus parasiticus

Jong-Gyu Kim........................................................................................................................... 457-462

Peptidoglycan hydrolases as novel tool for anti-enterococcal therapy

Lenka Maliničová, Mária Piknová, Peter Pristaš, and Peter Javorský.................................... 463-472

Seaweeds: A novel, untapped source of drugs from sea to combat Infectious diseases
S. Chanda, Dave R, Kaneria M and Nagani K.......................................................................... 473-480

How do bees prevent hive infections? The antimicrobial properties of propolis

H. Fokt, A. Pereira, A. M. Ferreira, A. Cunha, and C. Aguiar.................................................. 481-493

Novel Scientific Research in the College Classroom: Identification of Antibiotic

Resistant Pseudomonas Species from a Wastewater Treatment Plant
K. F. Picardo and D. W. Giroux................................................................................................ 494-500

Comparative analysis of 16S rRNA methyltransferases conferring resistance to

aminoglycoside antibiotics in producing strains
S. Vojnović, T. Ilić-Tomić, M. Savić, S. Bajkić, I. Morić, and B. Vasiljević.......................... 501-508

VI ©FORMATEX 2010
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A. Méndez-Vilas (Ed.)
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Antibiotic resistance dissemination and sewage treatment plants

K.L. Jury, T. Vancov, R.M. Stuetz, S.J. Khan.......................................................................... 509-519

Analysis of plasmids as genetic support of the class D carbapenemase OXA-40 in

multidrug-resistant Acinetobacter baumannii isolates
L. Gallego.................................................................................................................................. 520-528

Global resistance trends and the potential impact of Methicillin Resistant

Staphylococcus aureus (MRSA) and its solutions
S. Chanda, B.R.M. Vyas, Y. Vaghasiya, H. Patel..................................................................... 529-536

Bacillus spp. thermal resistance and validation in soups

A. Rodríguez-Lozano, M. Campagnoli, K. Jewel, F. Monadjemi and J.E. Gaze..................... 537-544

All Stressed Out: Mycobacterial Responses to Stress

Beatrice Saviola......................................................................................................................... 545-549

Defensin-like antifungal proteins secreted by filamentous fungi

L. Galgóczy, L. Kovács and Cs. Vágvölgyi.............................................................................. 550-559

Streptomyces developmental cycle and secondary metabolite production

A. Manteca and J. Sanchez........................................................................................................ 560-566

Endophytic fungi for producing bioactive compounds originally from their host plants
J. Zhao, L. Zhou, J. Wang, T. Shan, L. Zhong, X. Liu, and X. Gao......................................... 567-576

Evolution of fungal β-lactam biosynthesis gene clusters

Carlos García-Estrada, Francisco Fierro, and Juan-Francisco Martín...................................... 577-588

Medical & Veterinary Microbiology

Medical Genetics, Viruses
Microbes at the host surface
Sara K Linden and Michael A McGuckin................................................................................. 591-596

Chikungunya fever: A review of a re-emerging mosquito-borne infectious disease and

the current status
Po-Ying Chia, Mary Mah-Lee Ng and Justin Jang Hann Chu.................................................. 597-606

Cortactin, an oncoprotein targeted by pathogens during infection

E. Nieto-Pelegrín, E. Meiler, and N. Martínez-Quiles.............................................................. 607-614

Apparent impact of enteric pathogens in drinking water and implications for the
relentless saga of HIV/AIDS in South Africa
MNB Momba, E Madoroba and CL Obi................................................................................... 615-625

Campylobacter concisus: an emerging pathogen of the gastrointestinal tract

Taghrid Istivan, Peter Ward and Peter Coloe............................................................................ 626-634

Regulation of the LEE-pathogenicity island in attaching and effacing bacteria

C. Lara-Ochoa, R. Oropeza and A. Huerta-Saquero................................................................. 635-645

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 Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology
A. Méndez-Vilas (Ed.)
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The genomes of Group B Streptococcus and the relationship to pathogenesis of disease

H. H. L. Ma and M. Ip............................................................................................................... 646-657

Incidence of fungalysin and subtilisin virulence genes in dermatophytes

A. Lemsaddek, L. Chambel and R. Tenreiro............................................................................. 658-665

Bacteria and yeast normal microbiota from respiratory tract and genital area of
bottlenose dolphins (Tursiops truncatus)
R. Avalos-Téllez, F. Suárez-Güemes, E. M. Carrillo-Casas, and R. Hernández-Castro.......... 666-673

Lactobacilli and other lactic acid-related bacteria in the mucosal proximal

gastrointestinal tract of pigs: a review of ecology for two derivative approaches for
isolation of novel species
Mareike Kraatz.......................................................................................................................... 674-686

Leptospirosis: an important zoonotic diseasesis

Angeliki R. Burriel.................................................................................................................... 687-693

Experiences in Mexico of the vaccination in bovines and goats, with Brucella abortus
RB51 strain
E. Herrera-López , F. Suárez-Güemes, B. Arellano Reynoso, E.G. Palomares-Resendiz,
R. Hernández-Castro, and E. Díaz Aparicio............................................................................. 694-699

Bacterial biofilms: Importance in animal diseases

F. Aguilar-Romero, N.A. Pérez-Romero, E.Díaz-Aparicio and R. Hernández-Castro........... 700-703

Identification and Characterization of Novel Adhesins in Leptospira

M. V. Atzingen, G. M. Cerqueira, M. L. Vieira, R. Oliveira, T. R. Oliveira, R. F.
Domingos, A. T. Barros, R. S. Mendes and Ana L. T. O. Nascimento.................................... 704-713

The role of viruses in encephalitides of unknown origin in dogs

A. M. Amude, A. F. Alfieri, A. A. Alfieri.............................................................................. 714-722

Clinical courses and neurological signs of canine distemper virus infection in dogs
A. M. Amude, A. F. Alfieri, A. A. Alfieri................................................................................ 723-728

Non-conventional neuropathological manifestations of canine distemper virus

infection in dogs
A. M. Amude, A. F. Alfieri, A. A. Alfieri................................................................................ 729-736

Canine distemper virus and multiple sclerosis: A real or an anecdotal association?

A. M. Amude, A. F. Alfieri, A. A. Alfieri................................................................................ 737-745

Deciphering the infection biology of avian pathogenic Escherichia coli: role of

experimental infection models
Francis Dziva............................................................................................................................. 746-753

Vibrio parahaemolyticus Strain ATCC 17802 Contains Two Transcriptionally Active

16S rRNA Genes and neither degrades preferentially in starvation
N. González-Escalona, C.X. Moreno, J. Romero and R.T. Espejo........................................... 754-757

Differentiation and typing of Vibrio parahaemolyticus and V. vulnificus strains by

variability in their 16S-23S rRNA intergenic spacer regions
Narjol González-Escalona......................................................................................................... 758-766

VIII ©FORMATEX 2010

 Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology
A. Méndez-Vilas (Ed.)
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Aichi virus in Alberta, Canada: a one year examination of human diarrheic stool
samples by RT-PCR
A. Houde, E. Poitras, D. Leblanc, P. Ward, G.D. Inglis, V.F. Boras, F. Loisy-Hamon and B.
Lebeau....................................................................................................................................... 767-772

Preferential Assortment of Rearranged NSP3 Gene in the Genetic Background of

Simian Rotavirus SA11
Mohammed Mahbub Alam, Nobumichi Kobayashi, Masaho Ishino, and Souvik Ghosh........ 773-778

Laboratory surveillance of co-circulating respiratory viruses and enteroviruses during

the 2009 H1N1 influenza pandemic
J. Tan, and M.P. Carlos............................................................................................................. 779-788

©FORMATEX 2010 IX
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A. Méndez-Vilas (Ed.)
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VOL. 2

Introduction............................................................................................................................. XVII

Biofilms, Quorum Sensing

Manipulating bacterial cell fate: key role of surface-sensing and signal transduction
C. Dorel..................................................................................................................................... 791-800

Sugarcane and Grapevine Endophytic Bacteria: Isolation, Detection of Quorum

Sensing Signals and Identification by 16S v3 rDNA Sequence Analysis
A. O. Hudson, N. H. Ahmad, R. Van Buren, and M. A. Savka................................................ 801-806

Microbial biofilms: case reviews of bacterial and fungal pathogens persisting on

biomaterials and environmental substrata
Ian R. Cooper............................................................................................................................ 807-817

Multiple approaches to control dental unit water line biofilm contamination

Iram Liaqat and Anjum Nasim Sabri........................................................................................ 818-826

A Lactobacillus-derived biosurfactant inhibits biofilm formation of human pathogenic

Candida albicans biofilm producers
L. Fracchia, M. Cavallo, G. Allegrone, and M.G. Martinotti................................................... 827-837

The Perfect Adhesive

Stephen A. Klotz and Peter N. Lipke........................................................................................ 838-844

Advances in industrial biofilm control with micro-nanotechnology

Ferreira C., Pereira A. M., Melo L. F, Simões M..................................................................... 845-854

Biofilms: An Evolving and Universal Evasive Strategy of Bacterial Pathogens

S.Sekhar, M.Ohri, A.Chakraborti.............................................................................................. 855-859

Atomic force microscopy and optical microscopy: suitable tools for the study of the
initial stages of biofilm formation
Patricia Schilardi, Carolina Diaz, Constanza Flores, Florencia Alvarez, and Mónica
Fernández Lorenzo de Mele...................................................................................................... 860-869

Industrial & Food Microbiology, Biodegradation,

Production of chemicals and pharmaceuticals
Optimization of cell mass production of the probiotic strain Lactococcus lactis in batch
and fed-bach culture in pilot scale levels
N. Elmarzugi, H. El Enshasy, R. Abd Malek, Z. Othman, M. R. Sarmidi, and R. Abdel
Aziz........................................................................................................................................... 873-879

Current trends of ß-galactosidase research and application

S. Sheik Asraf and P. Gunasekaran........................................................................................... 880-890

X ©FORMATEX 2010
 Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology
A. Méndez-Vilas (Ed.)
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Characteristics and potential use of b-glucosidases from Zygomycetes

Judit Krisch, Miklós Takó, Tamás Papp, Csaba Vágvölgyi...................................................... 891-896

Lignocellulose as raw material in fermentation processes

S. I. Mussatto, and J. A. Teixeira.............................................................................................. 897-907

Improvement of wine organoleptic characteristics by non-Saccharomyces yeasts

L. Mendoza and M.E. Farías..................................................................................................... 908-919

Strategies for the enhancement of malolactic fermentation in the new climate

conditions
J.Vila-Crespo, J.M. Rodriguez-Nogales, E. Fernández-Fernández, and M.C. Hernanz-
Moral......................................................................................................................................... 920-929

Yarrowia lipolytica: an industrial workhorse

M.A.Z. Coelho, P.F.F. Amaral and I. Belo............................................................................... 930-944

Characterization of a thermostable α-glucosidase from Geobacillus thermodenitrificans

F84a
A. C. Cihan, B. Ozcan, N. Tekin, and C. Cokmus.................................................................... 945-955

Microbial Fuel Cells (MFCs) - a novel source of energy for new millennium
L. Veeranjaneya Reddy, S. Pradeep Kumar Young-Jung Wee................................................. 956-964

Botulinum neurotoxin: a deadly protease with applications to human medicine

K.M. Land and L.W. Cheng...................................................................................................... 965-971

Progresses on the knowledge about the ecological function and structure of the
protists community in activated sludge wastewater treatment plants
L. Arregui, B. Pérez-Uz, H. Salvadó and S. Serrano................................................................ 972-979

Decisionmaking in the development of a biocatalytic route for resolution of S-

naproxen – from screening to scale-up
LH Steenkamp and D Brady..................................................................................................... 980-987

Microbial ecology of anaerobic reactors for treatment of alcohol industry

wastewaters: a review
B.K. Đnce, N. Ayman-Öz, G. Türker, Ş. Çelikkol, O. Đnce....................................................... 988-999

Biocatalytic potential of thermophilic bacteria and actinomycetes

B.A. Kikani, R.J. Shukla, and S.P. Singh.................................................................................. 1000-1007

Nitroreductases: Enzymes with Environmental, Biotechnological and Clinical

Importance
Iuri Marques de Oliveira, Diego Bonatto and João Antonio Pêgas Henriques......................... 1008-1019

Strain and process development for citric acid production from glycerol-containing
waste of biodiesel manufacture
S.V. Kamzolova, S.G. Anastassiadis, A.R. Fatyhkova, N.P. Golovchenko, and I.G.
Morgunov.................................................................................................................................. 1020-1028

Design and Development of Microbial Fuel cells

Sourish Karmakar, Kanika Kundu and Subir Kundu............................................................... 1029-1034

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Competition Between Spontaneous And Commercial Yeasts In Winemaking: Study

Of Possible Factors Involved
N. Barrajón, M. Arévalo-Villena, J. Úbeda, and A. Briones.................................................... 1035-1041

Exploring the biodiversity of a wine region: Saccharomyces yeasts associated with

wineries and vineyards
L. Mercado, M. Combina.......................................................................................................... 1042-1053

Chitin deacetylase: A comprehensive account on its role in nature and its

biotechnological applications
V. Ghormade, S. Kulkarni, N. Doiphode, P.R. Rajamohanan and M.V. Deshpande............... 1054-1066

Microbial diversity within the phyllosphere of different vegetable species

Baoguo Zhang, Zhihui Bai, Daniel Hoefel, Xiaoyi Wang, Ling Zhang and Zuming Li.......... 1067-1077

Microbiological Control of Mycotoxins: Present Status and Future Concerns

K.R.N. Reddy, N.I. Farhana, B. Salleh and C.A.F. Oliveira..................................................... 1078-1086

Are consumers aware of the risks related to Biogenic Amines in food?

P. Russo, G. Spano, M.P. Arena, V. Capozzi, D. Fiocco, F. Grieco and L. Beneduce............. 1087-1095

Biology of Escherichia coli O157:H7 in human health and food safety with emphasis
on sublethal injury and detection
Amanda L. Robinson and John L. McKillip............................................................................. 1096-1105

Molecular Mechanisms of Stress Resistance in Lactococcus lactis

William M. Smith, Gary A. Dykes, Aijaz H. Soomro and Mark S. Turner.............................. 1106-1118

Dualistic aspects of Enterococcus spp. in foods

Bruna Carrer Gomes, Bernadette Dora Gombossy de Melo Franco and Elaine Cristina
Pereira De Martinis................................................................................................................... 1119-1125

Nutritional potential of biomass and metabolites from filamentous fungi

J.A. Takahashi and S.A. Carvalho............................................................................................ 1126-1135

Potential for manipulating the polysaccharide content of shiitake mushrooms

D. Brauer, T.E. Kimmons, M. Phillips, and D.E. Brauer........................................................ 1136-1142

Fresh-cut fruits preservation: current status and emerging technologies

M.R. Corbo, B. Speranza, D. Campaniello, D. D’Amato, and M. Sinigaglia........................... 1143-1154

Functional nutrition and psycho-intellectual possibilities of man: things in common

E.I. Tkachenko, Yu.P. Uspenskiy, Yu.A. Fominykh................................................................ 1155-1159

Advantages of traditional lactic acid bacteria fermentation of food in Africa

PK Chelule, MP Mokoena and N Gqaleni................................................................................ 1160-1167

Update in bread fermentation by lactic acid bacteria

G. Rollán, C.L. Gerez, A. M. Dallagnol, M.I. Torino and G. Font........................................... 1168-1174

Coliform bacteria in fresh vegetables: from cultivated lands to consumers

M.P. Falomir, D. Gozalbo, and H. Rico.................................................................................... 1175-1181

The problem of Listeriosis and ready-to-eat products: prevalence and persistence

V. Garrido, A.I.Vitas and I. García-Jalón................................................................................. 1182-1189

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Potential links between irrigation water quality and microbiological quality of food in
subsistence farming in KwaZulu-Natal, South Africa
Megan E. Gemmell and Stefan Schmidt................................................................................... 1190-1195

Production of Lactobacillus salivarius, a new probiotic strain isolated from human

breast milk, in semi-industrial scale and studies on its functional characterization
Roslinda Abd Malek, Sallehhuddin bin Hamdan, Hesham A. El Enshasy Nor Zalina
Othman, Noor Azwani Zainol, Mohamad R. Sarmidi, Ramlan, A. Aziz.................................. 1196-1204

Microbial safety of raw milk cheeses traditionally made at a pH below 4.7 and with
other hurdles limiting pathogens growth
F. Perez Pacheco, and A. Bucio Galindo.................................................................................. 1205-1216

Effect of modified atmosphere packaging on the growth/survival of Yersinia

enterocolitica and natural flora on fresh poultry sausage
C.A. Conte-Junior, B.T. Macedo, M.M. Lopes, R.M. Franco, M.Q. Freitas, M. Fernandez
and S.B. Mano........................................................................................................................... 1217-1223

S-layers in lactobacilli: structural characteristics and putative role in surface and

probiotic properties of whole bacteria
P. Mobili, E. Gerbino, E. E. Tymczyszyn, and A. Gómez-Zavaglia........................................ 1224-1234

Enhanced biodegradation of 4-chlorophenol using biosurfactant in an activated sludge

A. Uysal and A. Turkman......................................................................................................... 1235-1240

Removal of Ca2+ impurities from brine by marine cyanobacteria from Gujarat coast
of India for the production of Industrial grade salt
S Mishra, PK Ghosh, MR Gandhi, AM Bhatt and SA Chauhan............................................... 1241-1248

Preliminary characterization of an estuarine, benzoate-utilizing Vibrio sp. isolated

from Durban Harbour, South Africa
Karis Moxley and Stefan Schmidt............................................................................................. 1249-1254

Microorganisms capable to degrade organochlorine pesticides

María S. Fuentes, Claudia S. Benimeli, Sergio A. Cuozzo, Juliana M. Saez and María J.
Amoroso.................................................................................................................................... 1255-1264

Microbial cold-active α-amylases: From fundamentals to recent developments

Mohammed Kuddus, and Roohi................................................................................................ 1265-1276

Microbial biodegradation potential of hydrocarbons evaluated by colorimetric

technique: a case study
E. D. Bidoia, R. N. Montagnolli and P. R. M. Lopes................................................................ 1277-1288

Current Research Trends of Microbiological Leaching for Metal Recovery from

Industrial Wastes
Debaraj Mishra, Young-Ha Rhee.............................................................................................. 1289-1296

Adaptative response and degradation of quaternary ammonium compounds by

Pseudomonas putida A ATCC 12633
Gloria I. Lucchesi, Andrés S. Liffourrena, Paola S. Boeris and Mario A. Salvano.................. 1297-1303

Microbiological reduction of hexavalent molybdenum to molybdenum blue

Shukor M.Y. and M.A Syed...................................................................................................... 1304-1310

©FORMATEX 2010 XIII

 Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology
A. Méndez-Vilas (Ed.)
_______________________________________________________________________________________

Comparison of marine and terrestrial carbazole-degrading bacteria

Rintaro Maeda, Yoshihiko Ito, Kenichi Iwata and Toshio Omori............................................ 1311-1321

Green bean coffee as nutrient source for pesticide degrading-bacteria

B.E. Barragán-Huerta, and R. Rodríguez-Vázquez.................................................................. 1322-1327

Removal of organic load from wastewater by using Datura innoxia Mill.

N. Vaillant-Gaveau, F. Monnet, H. Sallanon, A. Coudret, and A. Hitmi................................. 1328-1336

The impact of different carbon and nitrogen sources on antibiotic production by

Streptomyces hygroscopicus CH-7
Slavica Ilić, Sandra Konstantinović, Vlada B. Veljković, Dragiša S. Savić and Gordana Đ.
Gojgić-Cvijović......................................................................................................................... 1337-1342

Mycofabrication, mechanistic aspect and Multifunctionality of Metal Nanoparticles -

Where are we? And where should we go?
Mahendra Rai, Alka Yadav, Aniket Gade................................................................................. 1343-1354

Third generation biofuels from microalgae

Giuliano Dragone, Bruno Fernandes, António A. Vicente, and José A. Teixeira.................... 1355-1366

Biosurfactants from marine bacterial isolates

R.Thavasi, S. Jayalakshmi and I.M. Banat................................................................................ 1367-1373

Citric acid production by yeasts: Fermentation conditions, process optimization and

strain improvement
Seda Karasu Yalcin, M. Tijen Bozdemir, Z. Yesim Ozbas....................................................... 1374-1382

Advances in butanol production by clostridia

Y. Tashiro, and K. Sonomoto.................................................................................................... 1383-1394

Bacterially Produced Polyhydroxyalkanoate (PHA): Converting Renewable Resources

into Bioplastics
Jiun-Yee Chee, Sugama-Salim Yoga, Nyok-Sean Lau, Siew-Chen Ling, Raeid M. M. Abed
and Kumar Sudesh..................................................................................................................... 1395-1404

Microbial production of biodegradable polymers and their role in cardiac stent

development
P. Basnett and I. Roy................................................................................................................. 1405-1415

Methods and Techniques

Design of experiments: a powerful tool in food microbiology
A. Bevilacqua, M.R. Corbo and M. Sinigaglia......................................................................... 1419-1429

Specific and Sensitive Multiplex PCR Method for Detecting Salmonellae and Shigellae
in Mayonnaise
E. Villalobo............................................................................................................................... 1430-1436

Introduction of PapilloCheck®, a microarray-based assay for the detection and

genotyping of HPV, into the clinical laboratory
D.A. Pilger, T.T. Moreira, D.M. Leinberger, and V.V. Cantarelli............................................ 1437-1442

XIV ©FORMATEX 2010

 Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology
A. Méndez-Vilas (Ed.)
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Sequencing a bacterial genome: an overview

J. Duan, J.J. Heikkila, B.R. Glick.............................................................................................. 1443-1451

Predictive microbiology and table olives

F.N. Arroyo-López, J. Bautista-Gallego, F. Rodríguez-Gómez,
and A. Garrido-Fernández......................................................................................................... 1452-1461

Disposable electrochemical biosensors in microbiology

O. Domínguez-Renedo, M.A. Alonso-Lomillo, M.J. Arcos-Martínez..................................... 1462-1470

Unraveling activities by functional-based approaches using metagenomic libraries

from dry and rain forest soils in Puerto Rico
José M. Cruz, Manuel A. Ortega, Jean C. Cruz, Pedro Ondina, Rossivette Santiago, and
Carlos Ríos-Velázquez.............................................................................................................. 1471-1478

Comparison of methods for the extraction of bacterial DNA from human faecal
samples for analysis by real-time PCR
E.A Nelson, E.A Palombo, and S.R Knowles........................................................................... 1479-1485

Ca-alginate hydrogel rheological changes caused by yeast cell growth dynamics

I. Pajic-Lijakovic, M. Plavsic, V. Nedovic, B. Bugarski........................................................ 1486-1493

The development of a robust industrial crystallography program for the bacterial 50S
ribosomal subunit
B.A. Maguire............................................................................................................................. 1494-1501

Tracking down microbial communities via fatty acids analysis: analytical strategy for
solid organic samples
M. Lores M. Gómez-Brandón and J. Domínguez..................................................................... 1502-1508

Application of Amplicon Length Polymorphism to differentiate amongst closely

related strains of bacteria
W. C. Rice................................................................................................................................. 1509-1516

Genetic tools for desiccation- and radiation-tolerant cyanobacteria of the genus

Chroococcidiopsis
D. Billi....................................................................................................................................... 1517-1521

PCR on slide: advantages in environmental and clinical microbiology

J.-C. Avarre, E. Masseret, M. Alunni-Fabbroni, P. Hartmann, W. Mann, N. Häfner, M.
Dürst, C. Woudstra, P. Fach, G. Cerovic, M. Rattier, A. Le bras, R. Melizzi, J.-L. Grabias
and C. Weisbuch........................................................................................................................ 1522-1532

Preparation and analysis of environmental DNA: optimisation of techniques for

phylogenetic analysis of ATAD sludge
A.V. Piterina and J.T. Pembroke............................................................................................... 1533-1538

Use of immunohistochemistry and molecular assays such as RT-PCR for precise post
mortem diagnosis of distemper-related encephalitis
A. M. Amude, A. F. Alfieri, A. A. Alfieri................................................................................ 1539-1545

Microbial Biodiversity Investigation techniques

S. Saman, P. Slattery and S. Saman.......................................................................................... 1546-1550

©FORMATEX 2010 XV
 Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology
A. Méndez-Vilas (Ed.)
_______________________________________________________________________________________

Challenges in Environmental Monitoring of Pathogens: Case Study in Mycobacterium

avium
N. Radomski, R. Moilleron, F. S. Lucas, and J. O. Falkinham III............................................
1551-1561
A modified cell extraction method to access microbial community structure in soil
samples by phospholipid fatty acid analysis
J. Malave-Orengo, S. E. Borglin, T. C. Hazen and C. Rios-Velazquez.................................. 1562-1568

Generation of large insert metagenomic libraries using indirect DNA extraction

methods from benthic and ephemeral tropical hypersaline microbial mats
I. Torres-Zapata, A. Gonzalez-Montalvo, C. Castro-Ruiz and C. Rios-Velazquez.................. 1569-1575

Protein- protein interaction mutants to study structure and assembly of cellular

machineries: its application to the prokaryotic divisome
L. Grenga................................................................................................................................... 1576-1581

Fourier tansform infrared (FT-IR) spectroscopy: A rapid tool for detection and
analysis of foodborne pathogenic bacteria
R. Davis and L.J. Mauer............................................................................................................ 1582-1594

UVA-LED Air Disinfection

M. Gadelmoula, K. Mawatari, M. Yamato, M. Akutagawa, Y. Kinouchi, and Akira
Takahashi................................................................................................................................... 1595-1600

Electron microscopy techniques and energy dispersive X-ray applied to determine the
sorption of lead in Paracoccus sp. DE2007
Massimiliano Baratelli, Juan Maldonado, Isabel Esteve, Antoni Solé and Elia Diestra........... 1601-1608

Others

Transport of dicarboxylates in Saccharomyces cerevisiae

D.A. Aliverdieva, D.M. Mamaev, L.S. Lagutina, and D.I. Bondarenko.................................. 1611-1620

XVI ©FORMATEX 2010