Quiz Set: #
Enzyme Kinetics and Immobilization
I. Identification.
Second order Lock-and-key theory Entrapment
Third order Michaelis-Menten Briggs-Haldane
Zero Order Cross Linking Adsorption
Enzymes Enzyme immobilization
1.) Biological catalysts that are protein in nature.
2.) Original theory to account for the formation of the
enzyme-substrate complex.
3.) In this approach that the product releasing step is much
slower than the reversible reaction and the slow step
determines the rate while the other is at equilibrium.
4.) This is also known as the pseudo-steady-state
assumption in chemical kinetics.
5.) When the substrate concentration is low, the reaction
rate is _____ with respect to the substrate.
6.) At high substrate concentration the rate of reaction is
______.
7.) The movement of the enzyme in a fixed location is
restricted by attaching them to an insoluble support
medium or enclosed by the support medium which is also
known as the carrier.
8.) This method of enzyme immobilization has a major
advantage in the fact that there is no chemical modification
of the enzyme.
9.) This method is based on the formation of covalent
bonds between the enzyme molecules by means of
multifunctional reagents, leading to three dimensional cross
linked aggregates.
10.) This procedure of immobilization is simple and it is
possible to separate and purify the enzymes.
II. Problem Solving.
1. A study was made to evaluate the constants so that the
Michaelis-Menten relationship could be used to describe
waste utilization by bacteria. It was found that one gram of
bacteria could decompose the waste at a maximum rate of
20 grams per day when the waste concentration was high.
Also, it was found that this same quantity of bacteria would
decompose waste at a rate of 10 grams/day when the waste
concentration surrounding the bacteria was 15 mg/l. The
rate of waste decomposition by 2 grams of bacteria if the
waste concentration were maintained at 5 mg/l is______.
2. Eadie (1942) measured the initial reaction rate of
hydrolysis of acetylcholme (substrate) by dog serum
(source of enzyme) and obtained the following data:
Evaluate the Michaelis-Menten kinetic parameters by
employing (a) the Langmuir plot, (b) the Lineweaver-Burk
plot, (c) the Eadie-Hofstee plot
Quiz Set: ##
Enzyme Kinetics and Immobilization
I. Identification.
Second order Lock-and-key theory Entrapment
Third order Michaelis-Menten Briggs-Haldane
Zero Order Cross Linking Adsorption
Enzymes Enzyme immobilization
1.) The movement of the enzyme in a fixed location is
restricted by attaching them to an insoluble support
medium or enclosed by the support medium which is also
known as the carrier. - enzyme immobilization
2.) This method of enzyme immobilization has a major
advantage in the fact that there is no chemical modification
of the enzyme. - entrapment
3.) This method is based on the formation of covalent
bonds between the enzyme molecules by means of
multifunctional reagents, leading to three dimensional cross
linked aggregates. - cross linking
4.) Biological catalysts that are protein in nature. - enzymes
5.) Original theory to account for the formation of the
enzyme-substrate complex. – lock-and-key theory
6.) This procedure of immobilization is simple and it is
possible to separate and purify the enzymes. - adsorption
7.) In this approach that the product releasing step is much
slower than the reversible reaction and the slow step
determines the rate while the other is at equilibrium.
8.) When the substrate concentration is low, the reaction
rate is _____ with respect to the substrate.
9.) At high substrate concentration the rate of reaction is
______.
10.) This is also known as the pseudo-steady-state
assumption in chemical kinetics.
II. Problem Solving.
1. Eadie (1942) measured the initial reaction rate of
hydrolysis of acetylcholme (substrate) by dog serum
(source of enzyme) and obtained the following data:
Evaluate the Michaelis-Menten kinetic parameters by
employing (a) the Langmuir plot, (b) the Lineweaver-Burk
plot, (c) the Eadie-Hofstee plot
2. A study was made to evaluate the constants so that the
Michaelis-Menten relationship could be used to describe
waste utilization by bacteria. It was found that one gram of
bacteria could decompose the waste at a maximum rate of
20 grams per day when the waste concentration was high.
Also, it was found that this same quantity of bacteria would
decompose waste at a rate of 10 grams/day when the waste
concentration surrounding the bacteria was 15 mg/l. The
rate of waste decomposition by 2 grams of bacteria if the
waste concentration were maintained at 5 mg/l is______.
Quiz Set: ###
Enzyme Kinetics and Immobilization
I. Identification
Second order Lock-and-key theory Entrapment
Third order Michaelis-Menten Briggs-Haldane
Zero Order Cross Linking Adsorption
Enzymes Enzyme immobilization
1.) This procedure of immobilization is simple and it is
possible to separate and purify the enzymes.
2.) This method is based on the formation of covalent
bonds between the enzyme molecules by means of
multifunctional reagents, leading to three dimensional cross
linked aggregates.
3.) This method of enzyme immobilization has a major
advantage in the fact that there is no chemical modification
of the enzyme.
4.) The movement of the enzyme in a fixed location is
restricted by attaching them to an insoluble support
medium or enclosed by the support medium which is also
known as the carrier.
5.) At high substrate concentration the rate of reaction is
______.
6.) When the substrate concentration is low, the reaction
rate is _____ with respect to the substrate.
7.) This is also known as the pseudo-steady-state
assumption in chemical kinetics.
8.) In this approach that the product releasing step is much
slower than the reversible reaction and the slow step
determines the rate while the other is at equilibrium.
9.) Original theory to account for the formation of the
enzyme-substrate complex.
10.) Biological catalysts that are protein in nature.
II. Problem Solving.
1. A study was made to evaluate the constants so that the
Michaelis-Menten relationship could be used to describe
waste utilization by bacteria. It was found that one gram of
bacteria could decompose the waste at a maximum rate of
20 grams per day when the waste concentration was high.
Also, it was found that this same quantity of bacteria would
decompose waste at a rate of 10 grams/day when the waste
concentration surrounding the bacteria was 15 mg/l. The
rate of waste decomposition by 2 grams of bacteria if the
waste concentration were maintained at 5 mg/l is______.
2. Eadie (1942) measured the initial reaction rate of
hydrolysis of acetylcholme (substrate) by dog serum
(source of enzyme) and obtained the following data:
Evaluate the Michaelis-Menten kinetic parameters by
employing (a) the Langmuir plot, (b) the Lineweaver-Burk
plot, (c) the Eadie-Hofstee plot
Quiz Set: ####
Enzyme Kinetics and Immobilization
I. Identification
Second order Lock-and-key theory Entrapment
Third order Michaelis-Menten Briggs-Haldane
Zero Order Cross Linking Adsorption
Enzymes Enzyme immobilization
1.) This is also known as the pseudo-steady-state
assumption in chemical kinetics.
2.) At high substrate concentration the rate of reaction is
______.
3.) When the substrate concentration is low, the reaction
rate is _____ with respect to the substrate.
4.) In this approach that the product releasing step is much
slower than the reversible reaction and the slow step
determines the rate while the other is at equilibrium.
5.) This procedure of immobilization is simple and it is
possible to separate and purify the enzymes.
6.) Original theory to account for the formation of the
enzyme-substrate complex.
7.) Biological catalysts that are protein in nature.
8.) This method is based on the formation of covalent
bonds between the enzyme molecules by means of
multifunctional reagents, leading to three dimensional cross
linked aggregates.
9.) This method of enzyme immobilization has a major
advantage in the fact that there is no chemical modification
of the enzyme.
10.) The movement of the enzyme in a fixed location is
restricted by attaching them to an insoluble support
medium or enclosed by the support medium which is also
known as the carrier.
II. Problem Solving.
1. Eadie (1942) measured the initial reaction rate of
hydrolysis of acetylcholme (substrate) by dog serum
(source of enzyme) and obtained the following data:
Evaluate the Michaelis-Menten kinetic parameters by
employing (a) the Langmuir plot, (b) the Lineweaver-Burk
plot, (c) the Eadie-Hofstee plot
2. A study was made to evaluate the constants so that the
Michaelis-Menten relationship could be used to describe
waste utilization by bacteria. It was found that one gram of
bacteria could decompose the waste at a maximum rate of
20 grams per day when the waste concentration was high.
Also, it was found that this same quantity of bacteria would
decompose waste at a rate of 10 grams/day when the waste
concentration surrounding the bacteria was 15 mg/l. The
rate of waste decomposition by 2 grams of bacteria if the
waste concentration were maintained at 5 mg/l is______.