M18 - Service Engineer Training

Mythic™18
Service Engineer Training

Workbook
03/01/2008 -1- Orphée S.A/M18/SEW/002

Proprietary Statement
Copyright 2006, Orphée S.A. All rights reserved. No part of this document
may be reproduced, stored in a retrieval system or transmitted in any
form or by any means, electronic, mechanical, photocopied, recorded, or
otherwise, without the prior written permission of Orphée S.A.
Service engineer training workbook usage disclaimer
Information contained in this document was developed for use by trained

Orphée S.A. service engineers personnel, or by other persons
knowledgeable or experienced with operation and service of the Mythic
system.
Trademark statements
Mythic is a trademark of Orphée S.A.
03/01/2008 -2- Orphée S.A/M18/SEW/002

Revision history Pages revised and
added
SEW/001 12/2006 First release
SEW/002 12/2007 Second release

Add version 2.0.0
specifications
03/01/2008 -3- Orphée S.A/M18/SEW/002

Mythic™18 Service Engineer Training Workbook
Index
Section 1……………………………Overview
Section 2…………………………..User Interface
Section 3…………………………..Mythic™18 Fluidic
Section 4…………………………..Mythic™18 Electronic
Section 5…………………………..Mythic™18 Mechanic
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Section 1: Overview
• Learning Objectives
• Front view
• Right side view
• Rear view
• Left side view
• Physical specifications
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Learning objectives
Section 1 will provide and overview of the system and physical

dimensions of the Mythic™18.
After reviewing Section 1 of this workbook you should be able to do

the following:
• Identify the connections located on the rear of the Mythic™18.

• Connect the right reagents on the right connectors.
• Identify the different fluidic module of the Mythic™18.
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Mythic™18 – front view
Power Switch
Numerical
LCD Display Keyboard
touchscreen
Status Indicator
LED
Start Cycle key
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Mythic™18 – Right Side View
Sampling module
Counting module
Syringes module
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Mythic™18 – Rear View
USB ports
TCP/IP port
Barcode
reader
Waste
connector RS232 port
Diluent
connector Centronic port
24 V Power
supply
External connector
grounding
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Mythic™18 – Left side view
Lyse solution
Straw
Cleaner Straw
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Mythic™18 – Physical Specifications
Physical dimensions
Dimension Instrument
Height 350 mm
Width 250 mm
Depth 340 mm
Weight 11 kg
Note: For complete listing of physical specifications, refer to

Mythic™18 System Operator’s Manual.
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Section 2: User Interface
• Learning Objectives.
• Operator Screens.
• Technician Screen.
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Learning Objectives
Section 2 will explain the user and service interface. After reviewing
this section of the workbook you should be able to do the following:
• List the main menus of the instrument.

• Describe how to use USB thumb drive.
• Describe how to calibrate the instruments.
• Describe how to restore Quality Control values.
• Describe how to connect the SVM.
• Describe how to check the pressure on the Mythic™18.
• Describe where to adjust the electronic gains.
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Operator Screens – Main tree
M ain M en u
ST A R T U P Launch new m enu

B la n k C y c le
L a u n c h M e c h a n ic a l a c tio n
SHU T DOW N E n try m e n u s

E n d o f d a y C y c le
V ie w R e s u lts
C A L IB R A T IO N
A u t o m a t o n c o e f. a d j u s t .
CHA NGE R ESU LT

T a r g e t v a lu e s e n t r y R u n s fo r c a l ib r a t io n
Q U A L IT Y C O N T R O L
QC
CHA NGE R ESU LT LJ

T a r g e t v a lu e s R u n s fo r c h e c k in g V ie w Le ve y
e ntry J e n n in g s
R E SU LT S
V ie w c h a r t r e s u lt s
RES V IE W
V ie w R E S n u m b e r V ie w fu ll s c r e e n
s e le c t e d h is t o r y
SET U P
A DVA NCED
( B io lo g is t p a s s w o r d )
P R IN T E R C O M M U N IC A T IO N A N L Y S IS LA B . C A L IB R A T IO N OT HERS
O P T IO N S PA RA MET ERS FA C T O R S S E T T IN G S
L IM IT S
T H R E SH OLD S
A LA R M S
BLOO D T Y P E
CHA NGE
L IM IT S
T H R ESH OLD S
A LA R M S
S E R V IC E C O R R E C T . FA C T O R S
C ONT R OL C Y C LE M O T O R IN IT H Y D R A U L IC S M E C H A N IC S R E P E A T A B IL IT Y LOG E R R OR S T E C H N IC IA N
G e ne ra l C o ntro l M e c h a n ic C o n t r o l R u n s c le a n in g R u n s m e c h a n ic a l R u n s fo r A u to m a to n s ta tu s T e c h n i c ia n
c y c le c y c le c y c le s c o n t r o l c y c le s r e p e a t a b ilit y c o n t r o l r e po r ting fu n c t io n a lit ie s
D R A IN A L L C H EC K V A LV ES
R ESET A LL
R IN S E A L L
C H EC K NEED LE
C L E A N IN G
CHECK ROCKER
B A C K FLU SH
C H E C K S Y R IN G E
B LEA C H
C HECK SENSOR S
OP ER A T OR LOG OU T IN IT P R E S S
Q u it a n d la u n c h n e w u s e r H G B LE D O FF
N EED LE
REA GENT S D IS M A N T L IN G
R e a g e n t fu n c t io n s
L U B R IC A T IO N
P OS.
D IL U E N T LYSE C LEA N ER W A ST E P R IM E A L L C Y C LES C OU N T ER
C H A NGE C H A NGE C H A NGE R ES ET

R EA GENT R EA GENT R EA GENT
P R IM E P R IM E
P R IM E L Y S E
D IL UENT C L EA NER
LOG S
A u t o m a t o n d ia r y
Launch new m enu

R U N SA M P LE
R u n a n d p a t ie n t L a u n c h M e c h a n ic a l a c tio n
id e n t ific a t io n
E n try m e n u s
V ie w R e s u lts
RUN N E X T ID
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Operator Screens – Login
1- Password:
• None : User – Lab tech

• 123 : Chief of lab
• 8SUP : Field Service Engineer
• 18ST : Super Technician
Note1: With 18ST password, everything is possible, no alert

message is displayed.
Note2: We recommend to use the 8SUP password during the
installation of a new instrument.
2- Language:
Choose the selected language with the scroll bar.
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How to use the USB key
The USB key must be formatted before any use. The format should be done
on the Mythic 18.
How to proceed?
1. Plug the USB key on
o One of the two USB port for the Mythic 18 equipped with normal USB port.
o The USB port on the lower position for the Mythic 18 equipped with small
USB port (like camera USB port).
2. Go to Set Up, Advanced menu, Storage Options.
3. Then, press USB thumb drive format.
4. When the format is finished a prompt will appear.
o If the USB key is not compatible, a prompt will appear to inform you. In
this way, try another one.
NOTE: The format is done in FAT16. For this reason it is not necessary to
equip the Mythic 18 with a USB key with a capacity higher than 2 Gb.
2 Gb capacity = Around 60.000 patients.
128 Mb capacity = Around 10.000 patients.
Once your USB key is formatted, you can start to work with it.
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Overview of the main tree of the USB key:
The serial number of the Mythic 18 is coded as : xxxxxx-yyyyyy

These two groups of 6 digits will be used to create private folders, only usable on one
instrument.
Root folder for Mythic 18 analyser
Common folders. The content of this part

can be used on all Mythic 18 instruments.
NOTE: These folders must be created
manually, with Explorer Windows for
example.
All of these folders and datas contained in

these folders are dedicated to the
instrument and can’t be transfer to
another instrument.
Each folder is automatically created by the
Mythic 18.
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Folders description:
1/ Common datas
COMMONS DATAS : \ROOT\

(sub)Folder files contents comments Format and Analyser
unit Write/Read
LOT MXC1234.QCN Qc target values One file per target ASCII
MBC02174.QDL Limited at 100 files STD unit
(target name.Test_Level) Calibration target NO / YES
CAL0233.CAL values
(target name.CAL)
SETTINGS USER.SET Common settings One file ASCII + UTF-8 YES / YES
UPDATE\ V100-004.b Software release One file per software release binary NO/YES
( version name.b)
VERSION
UPDATE\ HP6122.LPT Printer drivers One file per printer driver binary NO/YES
(prn_name.port LPT or USB )
PRTDRV
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2/ Mythic 18 dedicated data
PRIVATES DATAS : \ROOT\xxxxxx\yyyyyy\

(sub)Folder Files name (example) contents comments Format and Analyser
unit Write / Read
CALI 221006.CAL Calibration report = One file per calibration date ASCII + UTF-8
(calibration date as calibration factors + current unit YES / NO
DDMMYY.CAL) target + results
LOG LOG_ERR.CSV Error log Two files ASCII YES / NO
LOG.CSV service log
QC CRXV6875.QCN QC report = One file per QC target and level ASCII + UTF-8
(target name.level) target + results STD unit YES / YES
RESULTS\ 0047.CSV Result analysis One sub folder per Year/month (YYYY- ASCII + UTF-8
(sequence number. CSV) MM), current unit
YYYY-MM\
One sub folder per day (DD), YES / YES
DD One file per result
Limited to 510 files
SETTINGS TECH.SET Private analyzer settings One file ASCII + UTF-8
YES / YES
REAGENTS REAGLOGS.CSV Private reagents log One file ASCII
YES / YES
03/01/2008 - 19 - Orphée S.A/M18/SEW/002

Mythic™18 Service engineer training workbook
Once installed, you can use the USB key how you want.
Two cases:
5. The USB key is not connected on the instrument:
All the patient results will be saved on the internal memory of the instrument.
NOTE: The capacity of the internal memory is 1.500 patient results instead of 500 in the
previous version.
When the key will be locked on the instrument, the Mythic 18 will ask you if you want to
transfer the data on the USB. In this way, these data will be erased from the internal
memory.
6. The USB key is connected on the instrument:
If you have activated the option Real time runs save in the Storage Options, all the
results from the patient will be directly saved on the USB key.
NOTE: To avoid the problem of memory full, we recommend activating the option FIFO
Mode (First In, First Out) in the storage options. When the memory is almost full the first
result will be erased and replaced by the last one.
03/01/2008 - 20 - Orphée S.A/M18/SEW/002

New printer options:
With the upgrade 2.0.0-009 or higher, we provide you with a USB driver; this driver is a
PCL3 driver, common to many USB printers.
How to install this driver:
• Plug your USB key on a computer.

• Create the folder UPDATE in the root folder M18, and the sub-folder PRTDRV,
respecting the size of the letters.
• Upload the driver in the PRTDRV folder.
• Plug your USB key on the Mythic 18.
• Go to Set-Up.
• Select PRINTER 3 UPDATE.
• From the page displayed, select

the driver you need to install and
press LOAD.
• Validate the message of
confirmation.
• After uploading, go to PRINTER
and select the printer you
installed.
• To finish, select the format of the
paper.
o 12” = A4 format
o 11” = US Format
o 6” = A4 format, 2 results
per page
o 5,5” = US Format, 2 results
per page.
In the Advanced Set-up you can select 3 new options in the Printer menu:
• Automatic Print-out Analysis:

o Check box activated: ON
o Check box inactivated: ON DEMAND
• Automatic Print-out Start Up:
o Check box activated: the result of the background is automatically printed.
o Check box inactivated: No print.
• Automatic Print-out QC:
o Check box activated: QC run is automatically printed.
o Check box inactivated: QC run not printed.
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Archive of patients:
In the previous version, all the patients were archived in the same table from 1 to 500
(max.).
With this new version, each patient result is archived in list of the day with the following
architecture:
- YY/MM1
o dd1
o dd2
o etc …
- YY/MM2
o dd1
o dd2
o etc …
- etc …
Step 1:
To display one result for one patient, you just need to load
the list of the day while pressing the DATE button in the
ARCHIVE menu.
Step 2:
Select either “INTERNAL MEMORY” or “MASS
STORAGE”
INTERNAL MEMORY = Mythic 18 memory
MASS STORAGE = USB Drive.
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Step 3:
The screen is shared in two parts:

- On the left, the list of the year and months in the
memory,
- On the right part, the list of days for the selected
month.
By default, the current month is displayed.

Select the day while pressing on it then, press in
VIEW. The list of results for this day is displayed.
From the archive menu, it is possible to save some results from the internal memory to
the USB drive.
To proceed, press on TOOLS from the main screen of the archive menu, select the days
you want to save, press SAVE.
03/01/2008 - 23 - Orphée S.A/M18/SEW/002

New communication protocol
With the update version 2.0.0-009 or higher, it is now possible to connect the Mythic 18
either with RS232 cable or using TCP/IP protocol.
This new option is available in Set-up, Advanced, and Communications.
- Host serial = RS232 connection

- Host net = TCP/IP protocol
- SVM serial = to connect to SVM through RS232
connection.
- Once you select one option, you can define the

setting for each option.
o SERIAL PARAM. For RS232 connection
o NET. PARAM. for Network connection
- For the SERIAL PARAM., normally you don’t need

to modify the default setting.
- For the NET. Parameters, select UDP/IP or

TCP/IP protocol, then input the different
requested information about the host and the
instrument (Automaton).
- The MAC address is defined on the CPU board. If

no MAC address is displayed, please contact us.
Due to the new functionalities on the Mythic 18, please refer to the document
“Communication_protocol_v2.0.0_M18.pdf” to modify the communication protocol for the
existing connection.
03/01/2008 - 24 - Orphée S.A/M18/SEW/002

Operator Screens – Main Menu
Toolbar available in each

menu:
Menu: Go back to Main

Menu
Previous screen: Come
back to the previous
menu.
Tools: Print, Send,
Save, Delete.
Date/time: By pressing,
gives the following
informations.
Mythic S/N
Password
SW version
CPU Board n°
03/01/2008 - 25 - Orphée S.A/M18/SEW/002

Operator Screens – Reagents
Input
- The lot diluent
- Expiry date
- Volume of
container in
ml.
- Press Change
Reagent to
confirm.
Note: Either you change one reagent and you prime this reagent or you
change all the reagents and you prime all.
03/01/2008 - 26 - Orphée S.A/M18/SEW/002

Operator Screens – Calibration
1. 2. Input lot, targets, limits or press Load to

load these data from USB key.
3. Press Result and run calibrator

at least in triplicate.
Press Calibration to calibrate.
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Operator Screens – Quality controls
1. Choose one lot 2. Input lot, targets and limits or press load
to load a new batch from USB key
3. Run your control selecting Result
RESTORE is used to restore a lot and all of the results from USB key to Mythic memory.
03/01/2008 - 28 - Orphée S.A/M18/SEW/002

Operator Screens – Set Up
Only available with at least

biologist password.

Only available with technician
password.
03/01/2008 - 29 - Orphée S.A/M18/SEW/002

Operator Screens – Set Up
Default parameters :
- Shut down : 180 mn.

- Needle Up : 5 mn.
- Valid Autoclean : Activated
- Clean frequency : 80
Note: if the customer performs more than

100 tests per day, let the autoclean
activated.
The shut down inactivates the test counting
for autoclean.
Activate the reagent checking to check the

validation of the expiry date as well as the
remaining volume of the reagents.
• PRESSURE CHECKING option:
o This option allows to check the pressure:
During the counting.

On the beginning of the cycle when the drain of the baths is done.
o A flag, DEFAULT PRESSURE will appear if a default of pressure is detected.
o The default of pressure could be observed if:
One or more reagent is empty.

A default of greasing is observed (O-rings from the seal plate, or pistons).
03/01/2008 - 30 - Orphée S.A/M18/SEW/002

Operator screens – SVM Connection
Refer to the “new communication protocol” part.
Connect the RS232 cable between Mythic™18 and Computer.
The cable configuration should be as described below.
Mythic 18 PC
DB9 male DB9 Female
1 --------------------------------------------------------------------- 1 CD
2 --------------------------------------------------------------------- 2 RXD
3 --------------------------------------------------------------------- 3 TXD
4 --------------------------------------------------------------------- 4 DTR
5 --------------------------------------------------------------------- 5 GND
6 --------------------------------------------------------------------- 6 DSR
7 --------------------------------------------------------------------- 7 RTS
8 --------------------------------------------------------------------- 8 CTS
9 --------------------------------------------------------------------- 9 RI
03/01/2008 - 31 - Orphée S.A/M18/SEW/002

Technician Menu
This entire menu is available for all the users.
1- Control Cycle: To use after an

emergency stop.
2- Motor Init: To reinitialize the 3
steppers motors.
3- Hydraulics: To clean the system in case
of clogging.
4- Mechanics: To check the entire
mechanical part of the instrument.
5- Repeatability: To test the repeatability
of the instrument. (up to 20 replicates)
6- Log Errors: All the errors from the
instruments.
7- Technician: Only available with
technician password.(See next section)
03/01/2008 - 32 - Orphée S.A/M18/SEW/002

Technician Menu – Mechanics
Pressure:
- In the stand by mode, the pressure is 0.
- To check a leak of pressure:
o Run a cycle (Run Analysis)
o Stop the cycle by pressing the
ON/OFF button, when pistons are
in medium position.
o Go to Check Sensors.
o Check the value for Pressure.
o This value must be unchanged
along the time.
o If the pressure decreases, check
the tubing and the syringe block.
HGB sensor:
- If the LED is OFF, the value must be
around 0.
- If the LED is ON (turn ON in the previous
menu), the value must be between 850
and 950.
WBC and RBC sensors:
- In Stand by mode, the values are less
than 30. These values are measured
directly on the preamplifier board.
- If one or both values are higher (higher
than 100), check for interferences inside
the machine or check for an interference
source outside the machine.
03/01/2008 - 33 - Orphée S.A/M18/SEW/002

Technician Menu – Technician part
Note: This part is only available with the technician password: 8SUP
Only available with super technician

password.
Available to mainly change the lyse
volume
CO=Aperture Current.
To check the current inside the counting
bath.
1. Turn 0N by pressing the button.
2. Open the fluidic door.
3. Put a pin of the voltmeter inside the
courting bath, a pin to the ground.
4. Measure the voltage.
For WBC chamber, the value should be

around 9V.
For RBC/PLT chamber, the value should
be around 13V.
If the values are different, either change the
counting chamber or the CPU board.
03/01/2008 - 34 - Orphée S.A/M18/SEW/002

Technician Menu – Technician part
To adjust the electronic gains for WBC, RBC

and PLT.
To adjust the HGB blank.
See section Electronic part.
To clean the entire instrument before

packing.
Note: Never travel with liquid

inside the instrument.

03/01/2008 - 35 - Orphée S.A/M18/SEW/002

How to Upgrade Mythic™18 software from USB key?
1. When you receive a new upgrade, you receive a zip file like the following:
Mythic-2.0.1-003.zip
2. Extract all the files in the same folder.
3. Copy the file with the extension “.b” in the USB key in the folder VERSION.
4. Plug the USB on the Mythic 18.
5. Go to Set-Up/Advanced/Version Release.
6. Select the upgrade file and press load. The upgrade will be automatically
done.
03/01/2008 - 36 - Orphée S.A/M18/SEW/002

Section 3: Mythic™18 fluidic
• Fluidic diagram.
• The different modules.
o Sampling module
o Counting module
o Syringe module
• Troubleshooting on the fluidic part.
03/01/2008 - 37 - Orphée S.A/M18/SEW/002

Learning Objectives
Section 3 will explain the Mythic™18 fluidic. After reviewing this

section of the workbook you should be able to do the following:
• List the main modules of the instrument.

• Describe how to replace a sample probe.
• Describe how to change the O-rings.
• Describe how to fix a clogging problem.
• Describe how to diagnosis and fix a pressure leak.
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General fluidic diagram
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Fluidic cycles description:
Sampling cycle
1- Syringes down: (no valve)

~Less than 9,8 µl of blood sample are draw inside the sampling
needle
Diluent prime
Vacuum prime
2- Needle up and syringe up (valve 3+4 ON)

Needle outside cleaning with diluent and waste vacuum
3- Rocker move above the WBC bath and the syringes up to drain the
waste (valve 7 ON)
4- Needle down to the rinsing position
5- Syringe down
WBC bath drain (valve 1 ON)
6- Syringe up
Second outside needle cleaning (valve 4 ON)
Waste syringe drain (valve 7 ON)
7- Syringe down
8- Syringe up
03/01/2008 - 40 - Orphée S.A/M18/SEW/002

Sample dilution
WBC Dilution
9- Needle down to the dilution position and the syringes down to prime
the diluent (valve 8 ON)
10- Syringe up for the WBC dilution (~2 ml)

Diluent outside of the needle (valve 4 ON)
Diluent inside the needle (valve 4+5 ON)
Bubble to mix the dilution (valve 1 ON)
RBC dilution and lysing
11- Syringe down (no valve)

Drawing sample of the first dilution (~20µl)
Diluent prime
Vacuum prime
12- Needle up and syringe up

Rocker move above the RBC bath
Needle outside cleaning with diluent and waste vacuum (valve 3+4
ON)
13- Needle down to the rinsing position
14- Syringe up
Second outside needle cleaning (valve 4+8 ON)
Lysis in the WBC bath (0,3 to 0,5ml) (valve 9 ON)
Bubble to mix the dilution (valve 1 ON)
15- Syringe down

RBC bath drain (valve 2 ON)
16- Needle down to the dilution position
17- Syringe up for the RBC dilution (~1,5ml)

Diluent outside of the needle (valve 4+8 ON)
Diluent inside the needle (valve 5 ON)
Bubble to mix the dilution and diluent to rinse the counting head
(valve 6+10+2 ON)
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Measurement:
18- Syringe down to process the vacuum counting (valve 10 ON)
19- First measurement sequences (valve 10 ON)
20- Syringe up
21- Diluent back pressure (valve 4+6 ON)
22- Syringe down to perform the vacuum counting (valve 10 ON)
23- Second measurement sequences (valve 10 ON)
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Cleaning and drain
Drain and Rinse bath
24- Syringe down

Cleaning the apertures (valve 11 ON)
RBC bath drain (valve 2 ON)
25- Syringe up
RBC bath rinse diluent (valve 4 ON)
26- Needle up and syringes up for back flush (valve 4+6 ON)
27- Rocker move above the WBC bath
28- Syringe up
WBC bath rinse diluent (valve 4 ON)
Needle back and waste drain
29- Syringe down

Dry the outside of the needle (valve 3 ON)
30- Rocker move in the sampling position
31- Syringe up
32- Needle down in sampling position
03/01/2008 - 43 - Orphée S.A/M18/SEW/002

Fluidic modules: Sampling module
Rep Part Number Designation

1 005-1001-02 01 Sampling module
2 005-1001-02 03 Rocker Motor
3 005-1001-02 02 Needle Motor
4 005-1001-02 04 Needle rinsing head
5 010-2501-02 05 Sampling Needle
6 312-0505-140 125 O-ring Ø1.4x1.25 Fluocarbon 80SH
7 010-2501-02 02 Needle carriage
8 353-0301-08 120 19 Belt pitch 2.032 (l=4.763-L=243.84)
9 005-1001-82 880 300 Needle Optical Sensor
10 363-0201-030 080 Screw K 30 x 8
11 360-0102-030 060 Screw RLX M3 x 6
12 360-0101-030 080 Screw CZX M3 x 8
13 005-1001-82 880 200 Rocker Optical Sensor
14 370-4050-030 10 Anti-loss washer Ø3 x 1
03/01/2008 - 44 - Orphée S.A/M18/SEW/002

Fluidic modules: Counting module

1 005-1001-01 01 Counting chambers
2 010-2501-01 03 Counting manifold
3 005-1001-01 02 01 WBC Counting Head
4 005-1001-01 02 02 RBC Counting Head
5 312-0505-500 100 O-ring Ø5x1 Silicone 80SH
6 360-0101-030 060 Screw CZX M3 x 6
03/01/2008 - 45 - Orphée S.A/M18/SEW/002

Fluidic modules: Syringe module

1 002-1002-03 05 Syringes block
2 010-2501-03 13 Seal plate - Syringes module
3 010-2501-03 02 Piston Ø 16 - Syringes module
4 005-1001-03 03 Piston Ø 22
5 010-2501-03 01 Piston Ø 6.5 - Syringes module
6 005-1001-03 09 Syringe Piston Ø 1.6
7 312-0501-1554 262 O-ring Ø15.54x2.62 Silicone 60SH
11 010-2501-03 10 O-ring spacer
12 363-0201-030 100 Screw K 30 x 10
03/01/2008 - 46 - Orphée S.A/M18/SEW/002

Note1: For replacement of the O-rings on the syringe block, please refer to
the Mythic™18 service manual and the technical note.
Note2: The O-rings on the seal plate of the syringes module are greased in
the factory, as well as the pistons. It is very important that the greasing of
these parts is maintained all the time. We recommend greasing the
pistons every 6 months and the O-rings once per year. The pistons can
be greased by the customer.
A default of greasing could be suspected in the following cases of troubles:
- Trace of liquid under the syringes block.

- Leak of pressure.
- Abnormal noise during the movement of the pistons.
03/01/2008 - 47 - Orphée S.A/M18/SEW/002

Fluidic modules: Tubing connection
View n° 1 - Counting manifold
View n° 2 - Syringes manifold
View n° 3 - Syringes body
03/01/2008 - 48 - Orphée S.A/M18/SEW/002

Fluidic troubleshooting: Clogging
When could you suspect this problem?
A clogging could be suspected in the following cases:
- Rejected result on WBC, RBC, PLT.

- Drop of blood on the needle.
- Rejected result on WBC, RBC, PLT:
The result of one or more parameters appears as below:
WBC: --- *
RBC: --- *
PLT: --- *
Or
WBC: Value with “*”

RBC: Value with “*”
PLT: Value with “*”
In any case, the flags “W_CL” or “R_CL” appear in the flag box below the
results.
W_CL means WBC Clog.

R_CL means RBC/PLT clog.
To fix the problem:
1/ Rerun the sample.
2/ If the problem continues, follow the next procedure:
03/01/2008 - 49 - Orphée S.A/M18/SEW/002

Service Menu
Hydraulic
Cleaning
End of
OK Run Sample
service
NO
Backflush
OK
Run Sample
NO
Bleach with hypochloride

solution 10%
OK Run Sample
NO
1/ Dismantle the counting

head.
2/ Remove the o-ring.
3/ Place the counting head
OK in bleach solution for 10
minutes.
4/ Rinse the counting head
with distilled water
5/ Replace the counting
head
Replace the counting head

by a new one
Electronic gain adjustment
03/01/2008 - 50 - Orphée S.A/M18/SEW/002

- Drop of blood on the needle.
It could happen that you observe a drop of blood falling from the needle after
the blood aspiration.
Note: As we explain before, none of the valves is used during this part of the
cycle. So, it is not a problem of valve.
To fix the problem:
This problem appears when a clog is located in the rinsing head of the probe.
Rinsing
head
Tubing
n°10 :
Tubing n°21 :
Aspiration
Diluent tubing
tubing
Follow the next procedure:
03/01/2008 - 51 - Orphée S.A/M18/SEW/002

Remove the rinsing head from the rocker
1/ Dismantle the needle,

2/ unscrew the roller which maintains the O-ring,
3/ remove the O-ring.
4/ Disconnect the 2 tubing: n°10 and 21
1/ Place the rinsing head in hypochloride solution

for 5-10 minutes.
2/ Rinse it with distilled water
Replace the rinsing head and the needle.

Don't forget the o-ring
End of service OK Run Sample
NO
Replace the tubing n°10, aspiration tubing.
03/01/2008 - 52 - Orphée S.A/M18/SEW/002

Fluidic troubleshooting: Leak of pressure
- HCT result rejected or too low (not proportional with Hb result). Impossible
to recalibrate.
- Repeatability problem on the results.
How to fix the problem?
1- Diagnosis
Follow the next procedure:
03/01/2008 - 53 - Orphée S.A/M18/SEW/002

Login as Technician
Open the door of the instrument
Run analysis
Emergency stop when pistons on down position
1/ Service Menu
2/ Mechanics
3/ Check sensors
PRESSURE Check the value of the pressure. Should be stable along the time.
Check leak of liquid
STABLE If the pressure is decreasing, leak of pressure
PRESSURE UNSTABLE
Dismantle the seal plate of the syringes module.

1/ If the o-rings are old, replace them by new ones.
2/ If not, grease the o-rings.
Repeatability OK Run Sample
NO
HCT calibration OK Change valve n°4
End of service
03/01/2008 - 54 - Orphée S.A/M18/SEW/002

Fluidic troubleshooting: Bad repeatability on all parameters
- Bad volume of diluent : check electrovalve n°4

- Check if drain of WBC chamber is correct.
- Check if mixing by air inside WBC chamber seems correct: check if tubing
14 is clamped.
- Check tubing n°9 (restriction, clamp)
- Check electrovalve n°5 & 6.
- Syringe block is not correctly fixed on the syringe motor holder.
- If there is a small drop on the ending of probe at the end if the analysis
cycle, check alignment between the syringe piston diam. 1.6 mm and the
piston carriage.
Fluidic troubleshooting: Bad repeatability on RBC/HCT parameters
- Check if mixing by air inside RBC chamber seems correct: check if tubing
14 is clamped.
- Clean RBC/PLT aperture with a bleach solution and perform a backflush.
03/01/2008 - 55 - Orphée S.A/M18/SEW/002

Fluidic troubleshooting: Carry Over on PLT
- Replace DILUENT container.

- Run a bleach cycle.
- Check if drain of RBC chamber is correct (EV N°2, O-ring on chambers,
tubing 14 is clamped).
- Too much silicone grease on probe O-ring.
- Grounding connection is not tied on aperture.
03/01/2008 - 56 - Orphée S.A/M18/SEW/002

Fluidic troubleshooting: Leakage under chambers
- Check if chambers are correctly mounted on manifold.

- Check if 0-rings on chambers are correctly in place and greased.
Fluidic troubleshooting: Bad results
- WBC curve is abnormal:
o LYSE and CLEANER bottles are reversed.

o Electrovalve n°9 doesn’t work (always close)
o Tubing n°1 is clamped
- RBC and PLT values are multiplied by 2
o Electrovalve n°5 doesn’t work.
- RBC and PLT values are divided by 2
o Replace RBC/PLT aperture.
- RBC and PLT values equal to 0
o Clog on RBC/PLT aperture.

o RBC coaxial wire disconnected.
o Preamplifier board is out.
- WBC value equal to 0
o Clog on WBC aperture.

o WBC coaxial wire disconnected.
o Preamplifier board is out.
- Alarm +++D on WBC
o Check grounding on LYSE tubing 1.
03/01/2008 - 57 - Orphée S.A/M18/SEW/002

Fluidic troubleshooting: RBC & WBC Chambers overflow
- Tubing n°22 is disconnected from the pressure sensor.

- Electrovalve 8 or 3 doesn’t work.
03/01/2008 - 58 - Orphée S.A/M18/SEW/002

Section 4: Mythic™18 Electronic
• Electronic diagram.
• Boards description.
• Wiring description.
• Valves replacement.
• Electronic gain adjustment.
03/01/2008 - 59 - Orphée S.A/M18/SEW/002

Learning Objectives
Section 4 will explain the Mythic™18 Electronic part. After reviewing this
• List the 3 boards of the instrument.

• Describe how to replace a main board.
• Describe how to calibrate the touchscreen.
• Describe how to adjust the electronic gain.
03/01/2008 - 60 - Orphée S.A/M18/SEW/002

Mythic™18 Electronic: General electronic diagram
03/01/2008 - 61 - Orphée S.A/M18/SEW/002

Mythic™18 Electronic: Main Board – Main components
+12V DC
CMD Rocker
CMD Needle
+3.3V
CMD Syringe
EV
+ 5V CMD
Counting EV
Pulse
processing
DRA
logic
Printer driver microprocessor Flash memory
03/01/2008 - 62 - Orphée S.A/M18/SEW/002

Mythic™18 Electronic: Main Board – Voltage check points
+3.3 V DC
+ 5 V DC
+60 V DC
+2.5 V DC
03/01/2008 - 63 - Orphée S.A/M18/SEW/002

Mythic™18 Electronic: Preamplifier board
03/01/2008 - 64 - Orphée S.A/M18/SEW/002

Mythic™18 Electronic: GUI board & Calibration of the touchscreen
NOTE:
It could be useful to recalibrate the touchscreen. To do this calibration:
• Press in the same time on the numerical keyboard the key “.” and
“DEL”.
• A cross appears on the display.
• Press in the middle of the cross.
• Proceed as previously with the next cross which appears.
• When the calibration is done, the display comes back to its initial
status.
03/01/2008 - 65 - Orphée S.A/M18/SEW/002

Mythic™18 Electronic: General wiring
03/01/2008 - 66 - Orphée S.A/M18/SEW/002

Mythic™18 Electronic: Main wiring
Door switch
Syringe EV Needle Optical

Sensor
Counting EV
Rocker Optical
Sensor
Needle motor
Motor
syringe Rocker motor
Syringe Optical
Sensor
NOTE: For the wiring of the different parts (sensors, motors, valves …),
please refer to the Mythic™18 service manual.
03/01/2008 - 67 - Orphée S.A/M18/SEW/002

Mythic™18 Electronic: Valves replacement
Note:
In order to proceed to the valves replacement we recommend using the

following material:
• Dynamometric screwdriver 20-120 cNm (TOHNICHI RTD 120 CN or
equivalent)
• Torx plug 6
1. Counting chamber valves replacement
03/01/2008 - 68 - Orphée S.A/M18/SEW/002

Mythic™18 Electronic: Valves replacement
2. Syringes module valves replacement
WARNING
EV 3/2 Ø 1.6mm
03/01/2008 - 69 - Orphée S.A/M18/SEW/002

Mythic™18 Electronic: Electronic gain adjustment
Note1:
The electronic gain adjustment should be done after replacement of the

following parts:
• Main board
• Preamplifier board
• Counting chamber
• Counting heads
Note2:
The electronic gain adjustment doesn’t replace the calibration of the

parameters with a blood calibrator.
03/01/2008 - 70 - Orphée S.A/M18/SEW/002

1. WBC Latex (Diameter : 7µ)
Spare Parts Changed Action
PCB Board WBC Electronic Gain

Adjustment
Preamplifier Board WBC Electronic Gain

Adjustment
Counting Chamber WBC Electronic Gain

Adjustment
WBC counting head WBC Electronic Gain

Adjustment
2. RBC/PLT Latex (Mix 1/1 Diameter 3µ/5µ)
Spare Parts Changed Action
PCB Board RBC/PLT Electronic Gain

Adjustment
Preamplifier Board RBC/PLT Electronic Gain

Adjustment
Counting Chamber RBC/PLT Electronic Gain

Adjustment
RBC/PLT counting head RBC/PLT Electronic Gain

Adjustment
03/01/2008 - 71 - Orphée S.A/M18/SEW/002

You need to login in TECHNICIAN MODE (enter the TECHNICIAN password).
- Select the SERVICE option

- Select the TECHNICIAN option
- And select PROD ADJUST
GAIN
- Shake the WBC LATEX with a VORTEX system

- Place the tube under the sample needle and press LATEX WBC
- Wait the end of the cycle and check that values are inside the following range:
210 < WBC GAIN < 250

66 < V WBC < 72
03/01/2008 - 72 - Orphée S.A/M18/SEW/002

- Shake the RBC/PLT latex with a VORTEX system

- Place the tube under the sample needle and press LATEX RBC PLT
- Wait the end of the cycle and check that values are inside the following range:
210 < RBC GAIN < 252

40 < V RBC< 44
215 < PLT GAIN < 252

51 < V PLT< 55
- When it is OK press VALID and confirm SAVING IN MEMORY
03/01/2008 - 73 - Orphée S.A/M18/SEW/002

Section 5: Mythic™18 mechanic
• Syringe motor sub-assembly and greasing
• Sampling module and greasing
03/01/2008 - 74 - Orphée S.A/M18/SEW/002

Learning Objectives
Section 5 will explain the Mythic™18 mechanic part. After reviewing this
• List the main mechanical parts of the instrument.

• Describe how to grease the main part of the syringe module.
• Describe how to grease the sampling module
03/01/2008 - 75 - Orphée S.A/M18/SEW/002

Mythic™18 mechanic: Syringe motor sub-assembly
2 parts needs to be greased during the preventive maintenance:
- The endless screw with the grease “Lubriplate DS-ES” ref. 410-0502-01.
- The guide of the pistons holder with the grease “Plastic grease SPG35”
ref. 410-0503-01
Plastic Grease SPG 35
03/01/2008 - 76 - Orphée S.A/M18/SEW/002

Mythic™18 mechanic: Sampling module
2 parts could be greased during the preventive maintenance:
- Axis of the rocker c with the grease “Plastic grease SPG35” ref. 410-
0503-01
- The rail of the rocker d with the grease “Plastic grease SPG35” ref. 410-
0503-01
03/01/2008 - 77 - Orphée S.A/M18/SEW/002

Mechanical troubleshooting: Syringe Motor (gap error)
- Waste tubing is clamped

- Check electrovalve n°7
- Check if all the pistons are correctly greased.
- Check if the syringe holder rails are greased.
- Check the greasing of the endless screw.
Refer to the technical note for
greasing recommendations.
03/01/2008 - 78 - Orphée S.A/M18/SEW/002

M18 - Service Engineer Training

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M18 - Service Engineer Training

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Mythic™18

Service Engineer Training

03/01/2008 -1- Orphée S.A/M18/SEW/002

Service engineer training workbook usage disclaimer

Information contained in this document was developed for use by trained

Mythic is a trademark of Orphée S.A.

03/01/2008 -2- Orphée S.A/M18/SEW/002

SEW/001 12/2006 First release

SEW/002 12/2007 Second release

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Section 1 will provide and overview of the system and physical

After reviewing Section 1 of this workbook you should be able to do

• Identify the connections located on the rear of the Mythic™18.

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Start Cycle key

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Note: For complete listing of physical specifications, refer to

03/01/2008 - 11 - Orphée S.A/M18/SEW/002

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• List the main menus of the instrument.

03/01/2008 - 13 - Orphée S.A/M18/SEW/002

ST A R T U P Launch new m enu

SHU T DOW N E n try m e n u s

CHA NGE R ESU LT

CHA NGE R ESU LT LJ

D IL U E N T LYSE C LEA N ER W A ST E P R IM E A L L C Y C LES C OU N T ER

C H A NGE C H A NGE C H A NGE R ES ET

Launch new m enu

03/01/2008 - 14 - Orphée S.A/M18/SEW/002

• None : User – Lab tech

Note1: With 18ST password, everything is possible, no alert

Choose the selected language with the scroll bar.

03/01/2008 - 15 - Orphée S.A/M18/SEW/002

1. Plug the USB key on

2. Go to Set Up, Advanced menu, Storage Options.

3. Then, press USB thumb drive format.

4. When the format is finished a prompt will appear.

03/01/2008 - 16 - Orphée S.A/M18/SEW/002

The serial number of the Mythic 18 is coded as : xxxxxx-yyyyyy

Root folder for Mythic 18 analyser

Common folders. The content of this part

All of these folders and datas contained in

03/01/2008 - 17 - Orphée S.A/M18/SEW/002

COMMONS DATAS : \ROOT\

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PRIVATES DATAS : \ROOT\xxxxxx\yyyyyy\

03/01/2008 - 19 - Orphée S.A/M18/SEW/002

5. The USB key is not connected on the instrument:

6. The USB key is connected on the instrument:

03/01/2008 - 20 - Orphée S.A/M18/SEW/002

New printer options:

How to install this driver:

• Plug your USB key on a computer.

• From the page displayed, select

• Automatic Print-out Analysis:

03/01/2008 - 21 - Orphée S.A/M18/SEW/002

03/01/2008 - 22 - Orphée S.A/M18/SEW/002

The screen is shared in two parts:

By default, the current month is displayed.

During the counting.

One or more reagent is empty.