NIST Traceable UV/Vis/NIR Reference Sets: ISO/IEC-17025 Accredited ISO Guide 34

NIST Traceable
UV/Vis/NIR Reference Sets

SPRING 2015 ISO/IEC-17025 Accredited ISO Guide 34
USB Drive supplied with each set contains the reference certificate
and all vital information required
Why do you need to use reference materials?
Ultraviolet, visible and NIR spectrophotometers are the following purposes:
fundamentally both accurate and precise but it is vital to * Demonstration of satisfactory instrument performance to any
check instrument performance on a regular basis to ensure inspection or regulatory agency.
operation is within satisfactory parameters. References
* Detection of instrument malfunction.
provided by Starna are based on materials, which have been
used or produced by a National Metrology Laboratory (NMI), * Provide data to a service technician allowing detection of
such as the National Institute of Standards and Technology instrument faults and assistance in repair.
(NIST) or fundamental metrological principles. When used in Each spectrophotometer has specific operating characteristics
routine testing of instrument performance, a quality control based on the geometry and basic design of the optical system.
database of the operating characteristics for the specific These reference materials become your guide to the efficient
instrument can be built. This database can then be used for operation of your instrument.
What makes Starna reference materials unique?

All sealed, Glass Filters or Metal on Quartz reference the sets will last for many years. All NIST traceable sets can be
materials produced by Starna are “Ready to Use”. recertified.
References are supplied in either heat fusion sealed quartz
cells or, in the case of the Glass or Metal on Quartz Filters, in
Properties of Starna heat fusion sealed Quartz cells, glass or
an aluminum holder with the same external dimensions as a
Metal on Quartz references:
standard spectrophotometer cell.
1) Sealed cells ensure cleanliness, repeatability and reliability
Starna's sealed references are environmentally friendly as 2) They can be used multiple times over many years
they eliminate the need to open an ampoule, use it once and 3) The range covers the Far UV, UV, Visible and NIR
throw it away. Wet chemistry methods are no longer required 4) All calibrated & NIST traceable references can be recertified.
to make up the references from raw materials. With care,
Construction of reference materials

Starna has over forty-five years of experience pioneering many variables, which might be introduced using any other
the technology of sealing reference materials into quartz form of optical configuration or reference preparation. The
spectrophotometer cells. The technology allows the use references have a lifetime guarantee subject to specified
of materials in the UV range where Glass Filters are not conditions and are supplied in suitable storage containers. The
usable. The sealed reference cells have the same optical Glass Filters are made to the highest industry standards with
characteristics as cells used in normal analysis thus removing raw material of known characteristics.
UKAS ISO/IEC 17025 Accredited / NIST Traceable

NIST (National Institute for Standards and Technology in used in the context of standard operating procedures for
Washington DC, USA) traceability is available on all our quality control, established in your laboratory. Our calibration
reference materials, unless otherwise indicated. Traceability is laboratory is inspected and accredited by UKAS under ISO/
established during the calibration of each of our five reference IEC 17025 for the Starna calibration laboratory and under
spectrophotometers using NIST primary standards, both before
ISO Guide 34 as a reference material producer. This level
and after calibrating Starna reference materials. Documentation
supplied with Starna references details the analysis procedure, of accreditation assures the user that the certification of our
NIST traceability, the analyzed values of the reference and the reference materials is of the highest standard and may be
confidence limits. Certification supplied with Starna reference used to validate instrument performance in all ISO/IEC 17025
materials is suitable for use with all inspecting agencies when regulated environments.
Should I use NIST Traceable References?

Traceability to NIST allows for a definitive, certifiable method This certificate details the method of analysis and the certified
of analysis of the reference using the same methods as NIST. results of the analysis. You can use these certified results
Additionally, each reference raw material is either purchased as the basis of your quality program. If your lab is routinely
from NIST (where available) or directly tested against a NIST monitored by a certifying agency, then the Starna level of
supplied reference. The most important element of NIST accreditation and NIST traceability is invaluable.
traceability is the Starna certificate of analysis and traceability.
2 Starna Cells, Inc (800) 228-4482

Table of Contents:
Ultraviolet References
Material: Used for: Traceable to: Configuration: Page(s):
Potassium Dichromate Absorbance, Linearity, UV SRM 935a Filled Quartz Cell 4, 5, 6 & 7
Nicotinic Acid Absorbance, Far UV SRM 935a Filled Quartz Cell 8&9
Metal on Quartz Filters Absorbance, Linearity, UV/VIS SRM 2301 Metal Coated Quartz Window 10
Starna Green Absorbance, Wavelength, Linearity UV/VIS SRM 935a/2034 Filled Quartz Cell 11
Sodium Chloride Stray light Far UV SRM 2032 Filled Quartz Cell 18 & 19
Potassium Chloride Stray light Far UV SRM 2032 Filled Quartz Cell 18 & 19
Potassium Iodide Stray light UV SRM 2032 Filled Quartz Cell 18 & 19
Sodium Iodide Stray light UV SRM 2032 Filled Quartz Cell 18 & 19
Lithium Carbonate Stray light UV SRM 2032 Filled Quartz Cell 18 & 19
Sodium Nitrite Stray light VIS SRM 2032 Filled Quartz Cell 18 & 19
Acetone Stray light VIS SRM 2032 Filled Quartz Cell 18 & 19
Holmium Oxide Wavelength UV/VIS SRM 2034 Filled Quartz Cell 12
Didymium Oxide Wavelength UV/VIS SRM 2034 Filled Quartz Cell 13
Samarium Oxide Wavelength UV/VIS SRM 2034 Filled Quartz Cell 14
Rare Earth Oxide Wavelength Far UV SRM 2034 Filled Quartz Cell 15
Benzene Vapor Resolution not controlled Filled Quartz Cell 20
Toluene in Hexane Resolution SRM 935a Filled Quartz Cell 21
Visible References
Material Used for Traceable to: Configuration: Page:
Neutral Density Glass Filters Absorbance, Linearity, VIS SRM 930e Glass Filters 22, 23 & 25
Holmium Glass Filter Wavelength VIS SRM 2034 Glass Filter 24 & 25
Didymium Glass Filter Wavelength VIS SRM 2034 Glass Filter 24
Near Infrared
Metal on Quartz Filters Absorbance, Linearity, NIR SRM 2301 Metal Coated Quartz Window 16 NIR
Wavelength material Wavelength, NIR SRM 2065 Filled Quartz Cell 17
DNA and RNA 260/280 Ratio References

Stable Aqueous 260/280 Absorbance UV SRM 935a Filled Quartz Cell 28
Stable Aqueous 260/280 Absorbance UV SRM 935a Vials, Screw or Crimp top 29
Microplate Reader References

Glass Filters Wavelength, Absorbance, VIS SRM 2034, 930e Glass Filters 26
Adaptor To facilitate the use of standard sealed quartz cells or glass filters to validate Microplate Readers 27
Fluorescent & Raman References

Quinine Sulphate Solution Wavelength, Intensity & Linearity SRM 2034, 930e Filled Quartz Cell 30
Ultra High Purity Water Fluorescent Instrument Sensitivity Filled Quartz Cell 31
References for use with methods described in...

Material Test Range USP Ph. Eur. DAB BP ASTM TGA
Holmium Oxide Wavelength UV/Visible √ √ √ √ √ √

Toluene in Hexane Spectra Resolution UV √ √ √ √ √ √
Potassium Chloride Stray Light UV √ √ √ √ √ √
Sodium Iodide Stray Light UV √ √ √ √ √ √
Potassium Iodide Stray Light UV √ √
Potassium Dichromate Absorbance UV √ √ √ √ √ √
Neutral Density Filters Absorbance Visible √ √ √ √ √ √
www.starnacells.com 3
Potassium Dichromate - UV - Absorbance/Linearity up to 1.5A
Description & NIST Traceability: Potassium Dichromate from NIST (SRM 935a), permanently heat fusion sealed in quartz
cells, NIST Traceable complete with UKAS ISO/IEC 17025 accredited certificate of
calibration.
Primary Usage: Testing absorbance and linearity scale in the UV region of the spectrum up to 1.5
Absorbance.
Certified Wavelengths: 235, 257, 313 and 350 nm for 20mg/L to 100mg/L. 430 nm for 600mg/L.
Physical Configuration: UV quartz cells that have been permanently heat fusion sealed.
Product Description:
Potassium Dichromate solvated in dilute perchloric acid is The following chart is a listing of approximate absorbance
deemed to be the best method for testing the absorbance values for each concentration based on the wavelength at which
scale and linearity of spectrophotometers in the UV. Solid the Potassium Dichromate cells are to be read:
Potassium Dichromate used by Starna is purchased directly Concentration 235 nm 257 nm 313 nm 350 nm 430 nm
from NIST (SRM 935a). Solutions are made up in accordance
to NIST certification and then permanently heat fusion sealed 20 mg/L 0.243 0.281 0.095 0.209 n/a
in Far UV quartz spectrophotometer cells. 40 mg/L 0.492 0.572 0.192 0.426 n/a
The filled cells are tested and certified against the expected 60 mg/L 0.741 0.862 0.289 0.634 n/a
values from NIST. A certificate is provided with each set of 80 mg/L 0.996 1.159 0.385 0.853 n/a
cells, which has the certified absorbance values for each cell 100 mg/L 1.243 1.448 0.480 1.069 n/a
and the confidence limit. The certificate supplied is suitable European Pharmacopoeia Compliance requires the following
for use with inspecting agencies when used in the context of additional reference:
a standard operating procedure for quality control, established
in your laboratory. Each set is supplied with a sealed blank 600mg/L n/a n/a n/a n/a 0.950
cell used to obtain the certified measurements.
Suggestions for Use:

Scan the absorbance of the Potassium Dichromate cells over To evaluate instrument linearity, plot a graph of concentration
the certified wavelength range, against the blank supplied. against absorbance for each of the four wavelengths.
Compare the spectrophotometer absorbance values to the If you determine that your instrument is not giving you the
certified absorbance values. Taking the expanded uncertainty correct values, consult your service technician for advice on
budget† of the certified references into consideration, if the how to determine and correct any problem, which this reference
absorbance values fall within the expected parameters† of your may have detected.
instrument then your instrument is working correctly.
†For expanded uncertainty budget and expected parameters see page 35
Ordering Information:
Description Part Number, NIST Traceable Price
Linearity Dichromate set:
Potassium Dichromate, 5 concentrations & Blank Cell
20, 40, 60, 80 & 100mg/L & Blank Cell RM-0204060810 $ 2130.00
Single Concentrations:
Potassium Dichromate, 20 mg/L & Blank Cell RM-02 $ 830.00
Additional concentration required for European Pharmacopoeia Compliance:
Potassium Dichromate, 600 mg/L RM-60 $ 830.00
Linearity Dichromate set, with additional concentration for European Pharmacopoeia Compliance:
Potassium Dichromate, 6 concentrations & Blank Cell RM-020406081060 $ 2450.00

RM-0204060810 set:
This NIST traceable set of one blank and

five increasing concentrations of Potassium
Dichromate is our most useful and widely
purchased set consisting of 6 cells with the
following materials:
Cell# Containing Concentration

1 Perchloric acid blank 0.001N
2 Potassium Dichromate 20 mg/L
6 Potassium Dichromate 100mg/L
Spectral scan:
The graph to the left is a scan of 10 different
Potassium Dichromate Solutions
concentrations of Potassium Dichromate. It
clearly shows the peaks and troughs in relation to
absorbance and the differences due to Potassium
2.0000
Dichromate concentration.
Absorbance (A)
1.5000 100 mg/l Peaks: 257 and 350 nm

80 mg/l Troughs: 235 and 313 nm
1.0000 60 mg/l
40 mg/l The certificate supplied with the NIST traceable
0.5000
20 mg/l sets lists the absorbance values for each peak and
0.0000
trough at the four wavelengths measured against
220.0 240.0 260.0 280.0 300.0 320.0 340.0 the perchloric acid blank at the four wavelengths.
Wavelengths (nm) When measured in your spectrophotometer you can
validate your transmittance/absorbance scale for
accuracy and linearity using the set with 5 different
concentrations and the blank.
Linearity: Potassium Dichromate Linearity

Plotting concentration
against absorption using 1.5
the set of 5 cells against
the blank, you can test
your spectrophotometer 257 nm
Absorbance (A)
1
for linearity through the UV 235 nm
range.
350 nm
The use of the NIST 0.5 313 nm
traceable set with 5
different concentrations
and a blank is
recommended for best 0
results. 20 mg/l 40 mg/l 60 mg/l 80 mg/l 100 mg/l
Dichromate Concentrations
Description & NIST Traceability: Potassium Dichromate from NIST (SRM 935a), permanently heat fusion sealed in quartz
cells, NIST Traceable complete with UKAS ISO/IEC 17025 accredited certificate of
calibration.
Primary Usage: Testing absorbance and linearity scale in the UV region of the spectrum up to 3.3
Absorbance.
Certified Wavelengths: 235, 257, 313 and 350 nm for 20mg/L to 240mg/L.
Physical Configuration: UV quartz cells that have been permanently heat fusion sealed.
Potassium Dichromate solvated in dilute perchloric acid is The following chart is a listing of approximate absorbance
deemed to be the best method for testing the absorbance values for concentrations 120mg/l or higher, based on the
scale and linearity of spectrophotometers in the UV. Solid wavelength at which the Potassium Dichromate cells are to be
Potassium Dichromate used by Starna is purchased directly read:
from NIST (SRM 935a). Solutions are made up in accordance Concentration 235nm 257 nm 313 nm 350 nm
to NIST certification and then permanently heat fusion sealed
in Far UV quartz spectrophotometer cells. 120 mg/L 1.501 1.749 0.582 1.290
140 mg/L 1.758 2.050 0.679 1.506
The filled cells are tested and certified against the expected
values from NIST. A certificate is provided with each set of 160 mg/L 2.016 2.351 0.777 1.723
cells, which has the certified absorbance values for each cell 180 mg/L 2.275 2.655 0.875 1.940
and the confidence limit. The certificate supplied is suitable for 200 mg/L 2.536 2.960 0.974 2.157
use with inspecting agencies when used in the context of a
standard operating procedure for quality control, established in 220 mg/L 2.790 3.256 1.071 2.373
your laboratory. Each set is supplied with a sealed blank cell 240 mg/L 3.043 3.552 1.168 2.589
that was used to obtain the certified measurements.

Scan the absorbance of the Potassium Dichromate cells over To evaluate instrument linearity, plot a graph of concentration
the certified wavelength range against the blank supplied. against absorbance for each of the four wavelengths.
certified absorbance values. Taking the expanded uncertainty correct values, consult your service technician for advice
budget† of the certified references into consideration, if the on how to determine and correct any problem, which this
absorbance values fall within the expected parameters† of your reference may have detected.
Linearity Dichromate set from 1.5 to 3.5A
120, 140, 160, 180 & 220 & 240mg/L & Blank RM-121416182224 $ 2450.00
Linearity Dichromate set from 0.19 to 3.5A
40, 80, 120, 160, 200 & 240 mg/L & Blank RM-040812162024 $ 2450.00
Customer specified Potassium Dichromate Concentration options, including those up to 1.5A from page 4:
Potassium Dichromate, 1 concentration & Blank $ 830.00
Potassium Dichromate, 2 concentrations & Blank $ 1155.00

RM-121416182224 set:
This NIST traceable set of one blank and

six increasing concentrations of Potassium
Dichromate is our most useful and widely
purchased set consisting of 7 cells with the
following materials:

1 Perchloric acid blank 0.001N
Spectral scan:
The graph to the left is a scan of 10 different
Potassium Dichromate Solutions concentrations of Potassium Dichromate. It
3.0000
clearly shows the peaks and troughs in relation
200 mg/l
to absorbance and the differences due to
2.5000 180 mg/l
Potassium Dichromate concentration.
160 mg/l
2.0000
Peaks: 257 and 350 nm
Absorbance (A)
140mg/l
120 mg/l
Troughs: 235 and 313 nm
1.5000 100 mg/l
1.0000
80 mg/l
The certificate supplied with the NIST traceable
60 mg/l
sets lists the absorbance values for each
0.5000
40 mg/l
peak and trough at the four wavelengths
20 mg/l
measured against the perchloric acid blank
0.0000 at the four wavelengths. When measured in
220.0 240.0 260.0 280.0 300.0 320.0 340.0 your spectrophotometer you can validate your
Wavelengths (nm) absorption scale for accuracy and linearity using
the set with 5 different concentrations and the
blank.
Potassium Dichromate Linearity

Linearity:
Plotting concentration 4
against absorption using 3.5
the set of 5 cells against
3
the blank, you can test 257 nm
Absorbance (A)
your spectrophotometer 2.5

235 nm
for linearity through the
2
UV range. 350 nm
1.5
The use of the NIST 313 nm
1
traceable set with 5
different concentrations 0.5
and a blank is 0
recommended for best 20 mg/l 40 mg/l 60 mg/l 80 mg/l 100 mg/ 120 mg/ 140 mg/ 160 mg/ 180 mg/ 200 mg/ 220mg/l 240 mg/
results.
Dichromate Concentrations
Nicotinic Acid - Far UV - Absorbance/Linearity
Description & NIST Traceability: Nicotinic Acid, Far UV Absorbance standard, sealed in quartz cells, NIST Traceable
complete with UKAS ISO/IEC 17025 accredited certificate of calibration
Primary Usage: Testing absorbance scale and linearity in the Far UV region of the spectrum.
Certified Wavelengths: 213 nm, 261 nm
Physical Configuration: UV quartz cells that have been permanently heat fusion sealed
The use of Nicotinic Acid solvated in dilute hydrochloric is established in your laboratory. Each set is supplied with a
acid is a well documented method for the validation of the sealed blank cell that was used for the certified measurement.
absorbance scale and linearity of a spectrophotometer in
The following chart is a listing of approximate absorbance
the Far UV region. When prepared in 0.1M hydrochloric
values for each concentration based on the wavelength at which
acid, Nicotinic Acid gives a spectral scan containing
the Nicotinic Acid cells are to be read at 1 nm SBW*:
characteristic peaks at approx. 210 nm and 260 nm. Within
the concentration range 5-25 mg/l, if the absorbance scale Concentration 213 nm 261 nm
of a narrow SBW (2 nm) spectrophotometer is linear, the

apparent absorbances of a series of concentrations will be a
linear function of concentration, at a specified SBW. 6 mg/L 0.243 0.281
The filled cells are tested and certified against the calculated 12 mg/L 0.492 0.572
values. A certificate is provided with each set of cells which
18 mg/L 0.741 0.862
has the certified absorbance values for each cell and the
confidence limit. The certificate supplied is suitable for use 24 mg/L 0.996 1.159
with inspecting agencies when used in the context of a
standard operating procedure for the quality control that * Alternative SBW values available on request.

Scan the absorbance of the Nicotinic Acid cells over the To evaluate instrument linearity, plot a graph of concentration
certified wavelength range against the blank supplied. against absorbance for each of the two wavelengths.
certified absorbance values. Taking the expanded uncertainty correct values, consult your service technician for advice
budget† of the certified references into consideration, if the on how to determine and correct any problem, which this
absorbance values fall within the expected parameters† of your reference may have detected.
Linearity Nicotinic Acid set:
Nicotinic Acid, 4 concentrations & Blank Cell RM-1A2A3A4A $ 1980.00
Single Concentrations:
Nicotinic Acid , 6 mg/L & Blank Cell RM-1A $ 880.00
Nicotinic Acid, 12 mg/L & Blank Cell RM-2A $ 880.00


Nicotinic Acid - Far UV - Absorbance/Linearity
RM-1A2A3A4A set:
This NIST traceable set of one blank and four increasing

concentrations of Nicotinic Acid is unique in its ability for the
verification of the Far UV absorbance scale. It consists of 5 cells
with the following materials:

1 Hydrochloric acid blank 0.1M
2 Nicotinic Acid 6 mg/L
Spectral scan:
Nicotinic Acid Solutions The graph to the left is a scan of a set of the 4
1.2 different concentrations of Nicotinic Acid. It shows
the peaks clearly in relation to absorbance and the
1 differences due to Nicotinic Acid concentration.
0.8 24 mg/l
Peaks: 213 and 261 nm
Absorbance (A)
18 mg/l
0.6
12 mg/l
The certificate supplied with these NIST traceable
0.4 6 mg/l references shows the absorbance values for each
peak when measured against the hydrochloric
0.2
acid blank, at the two wavelengths. When
measured in your spectrophotometer you can
validate your transmittance/absorbance scale for
0
210 220 230 240 250 260 270
accuracy and linearity using the set with 4 different
Wavelength (nm)
concentrations and the blank.
Far UV Linearity
Assessment: Nicotinic Acid Linearity
By plotting concentration 1.4
against absorption when
measuring the set of 4 1.2
Nicotinic Acid cells against
the blank, you can test your 1
spectrophotometer for linearity

Absorbance (A)
0.8 213nm
through the Far UV range.
261nm
0.6
0.4
0.2
0
6 mg/L 12 mg/L 18 mg/L 24 mg/L
Concentrations (mg/l)
Metal on Quartz UV/Vis Absorbance/Linearity
Description & NIST Traceability: Metal on Quartz Filter for absorbance and linearity in the UV and Visible area of the
spectrum. NIST Traceable complete with UKAS ISO/IEC 17025 accredited certificate of
calibration
Certified Wavelengths: 250, 280, 340, 360, 400, 465, 500, 546.1, 590 and 635 nm at Spectral Band Width of 5 nm
or less.
Physical Configuration: Metal coated quartz filter with coated side protected by an optically contacted quartz
window. The filters are mounted in protective aluminum holders that will fit a standard
instrument cell holder.
The Starna RM-1Q3Q9Q is a direct equivalent to the NIST
SRM 2031, with a standard range of Metal on Quartz Metal on Quartz
absorbance standards for the UV/Visible area of the spectrum. 2.5
All Starna Metal on Quartz Filters have an optically contacted

Quartz Cover Plate to protect the metalised surface and
2.0 Q1
facilitate easy cleaning. This was also a feature of the original Q3
NIST SRM 2031.

1.5
1Q
Abosrbance (A)
3Q
The filters 1.0
allow
5Q
Absorbance
9Q
0.5
and Linearity
at the ten 0.0
wavelengths
0
0
20
25
30
35
40
45
50
55
60
65
70
75
80
85
across the Wavelength (nm)
range and the

relative values Available Metal on Quartz Filters:
are shown RM-1Q3Q9Q Part No. %T Transmittance Absorbance
on the table (SRM 2031 Equivalent) Q1 1% 2.00 A
below. They Q3 3% 1.52 A
can be certified at wavelengths other than those shown above, 1Q 10% 1.00 A
on request. The filters are produced in our ISO 9001 accredited
production facility, under ISO Guide 34 as a reference material
3Q 30% 0.52 A
producer. Calibration is carried out in our ISO/IEC 17025 5Q 50% 0.30 A
accredited Calibration Laboratory. 9Q 90% 0.06 A

The UV/Visible Metal on Quartz Filters have a relatively flat absorbance values fall within the expected parameters† of your
profile across all UV/Visible wavelengths. Normally three instrument then your instrument is working correctly.
representative absorption filters would be chosen for any given
It is important to confirm that Metal on Quartz Filters are
application but the filters may be bought individually, in sets as
suitable for use with your instrument as they have a highly
shown below or any combination from the standard range.
reflective surface and some instruments are sensitive to back
Compare the spectrophotometer absorbance values to the reflection within the sample compartment.
certified absorbance values. Taking the expanded uncertainty
budget† of the certified references into consideration, if the
Ordering Information (Let us know what combination you require):

Metal on Quartz Reference, 30%, & Blank Holder RM-3Q $ 1290.00
Metal on Quartz Reference, 3%, 50%, & Blank Holder RM-Q35Q $ 1890.00
Metal on Quartz Reference, 10%, 30%, 90%, & Blank Holder RM-1Q3Q9Q (NIST 2031a) $ 2850.00

Starna Green Broadband - Wavelength, Absorbance/Linearity
Description & NIST Traceability: A mixed ultra stable dye solution permanently heat fusion sealed in quartz cells. NIST
traceable complete with UKAS ISO/IEC 17025 accredited certificate of calibration
Primary Usage: Testing wavelength accuracy, absorbance scale and linearity in UV/Vis region.
Certified Wavelengths: 257, 416, and 630 nm with a SBW up to 12 nm
Physical Configuration: Far UV quartz cell that has been permanently heat fusion sealed.
The use of stable aqueous dye solutions is an established and absorbance scale of the spectrophotometer is linear, the
well recognized method for the validation of the absorbance apparent absorbencies of a series of concentrations will be a
scale and linearity of a spectrophotometer. linear function of concentration.
When prepared at the appropriate concentrations, these The Starna Green reference set of proven stability is supplied
references have spectral characteristics that allow both the with a Certificate of Calibration valid for 2 years by the Starna
certification of the wavelength (257, 416, and 630 nm) and Calibration Laboratory, which is accredited by UKAS under
absorbance scale (0.25, 0.5, 0.75 or 1.0 A) in one discrete ISO/IEC 17025, and ISO Guide 34.
reference. Within the concentration range available, if the
Starna Green
2.00
1.80
1.60
1.40
1.20 4S - 100
Absorbance (A)
1.00 3S - 75
0.80
2S - 50
0.60
1S - 25
0.40
0.20
0.00
200 300 400 500 600 700
Wavelengths (nm)
Starna Green, Blank, 25X concentration RM-1SG $ 770.00
Starna Green, Blank, 4 concentrations RM-1SG2SG3SG4SG $ 1720.00
Holmium Oxide - UV/Vis - Wavelength Reference
Description & NIST Traceability: Holmium oxide (4%) in perchloric acid (10%) sealed in a quartz cell, NIST Traceable
Primary Usage: Verification of wavelength scale accuracy in both UV and Visible
Certified Wavelengths: 241.0, 249.9, 278.2, 287.1, 333.5, 345.6, 361.4, 385.4, 416.1, 451.1, 467.8, 485.2,
536.5, 640.6 nm
Spectral Band Width: The certificate indicates the above wavelength measured at 0.1, 0.25, 0.5, 1, 1.5, 2,
and 3 nm. Other SBWs can be provided on request.
Physical Configuration: Filled Far UV quartz cell that has been permanently heat fusion sealed
Holmium has been used as a
wavelength standard for many Holmium Oxide Solution
years in both a doped glass filter 1.2
and in liquid format. Starna has
extended the use by creating 1
a filled and permanently heat

fusion sealed far UV quartz cell 0.8
which allows it to be used into

Absorbances (A)
the UV. 0.6
Offering sharp, stable peaks 0.4
over the range of 240 to 650 nm

Holmium perchlorate is used 0.2
to confirm that the wavelength

scale of your instrument is within 0
230 280 330 380 430 480 530 580 630
the manufacturer's tolerances Wavelengths (nm)
for the actual wavelength being

measured.
The use of the liquid filled quartz
cell ensures that the optical
configuration method for quality
control is exactly the same as for
a normal analysis.

Holmium presents a wide range of sharp resolvable peaks, be required to check or adjust your instrument. Holmium is
which are easily used to compare the wavelength indication measured against air as a blank makes no difference to the
on your spectrophotometer to the known peak. The first certified values. It is not recommended or good practise to
procedure should be to scan the cell over the usable range evaluate absorbance using a wavelength reference.
to assure that all peaks can be resolved. Adjust the slit
Spectra should be checked regularly, at a minimum in the area
width (SBW) if possible and scan rate to produce a usable
of regular analysis. Periodic use of the Holmium reference will
spectra. Check each peak to assure that the reading on your
enable the user to build a data log of the instrument’s spectral
spectrophotometer is within the manufacturer's tolerances
accuracy for use with certification and troubleshooting should
of the wavelength readout. If not, a service technician may
the correlation change over time.
Holmium, Wavelength reference cell, NIST traceable RM-HL $ 550.00

Didymium Oxide - Visible - Wavelength Reference
Description: Didymium Oxide in perchloric acid, sealed in a quartz cell, NIST Traceable complete with
UKAS ISO/IEC 17025 accredited certificate of calibration
Primary Usage: Verification of wavelength scale accuracy in the Visible spectrum
Certified Wavelengths: 298.0, 329.0, 354.0, 444.1, 468.7, 481.8, 512.0, 521.3, 574.9, 731.8, 740.1, 794.1,
801.2, 865.0 nm
Spectral Band Width: The certificate indicates the above wavelength measured at 0.1, 0.25, 0.5, 1, 1.5, 2
Physical Configuration: Filled far UV quartz cell that has been permanently heat fusion sealed
Didymium is a combination of two chemical elements, Praseodymium and Neodymium and
has been used as a visible wavelength standard in the form of doped Glass Filters.
Starna provides a quartz cell filled and permanently heat fusion sealed with a Didymium
Perchloric solution that will facilitate more effectively the evaluation of your instrument.
Didymium offers sharp, stable peaks over the range of 290 to 870 nm. The primary use
of Didymium is to confirm that the wavelength scale of your instrument is within the
manufacturer's tolerances for the actual wavelength being measured.
The use of the liquid filled quartz cell ensures that the optical configuration method for
quality control is exactly the same as for a normal analysis.
Didymium
2
1.8
1.6
1.4
1.2
Absorbance (A)
1
0.8
0.6
0.4
0.2
0
290.00 390.00 490.00 590.00 690.00 790.00
Wavelengths (nm)

Didymium presents a wide range of sharp resolvable peaks, be required to check or adjust your instrument. Didymium is
width (SBW) if possible and scan rate, to produce a usable
of regular analysis. Periodic use of the Didymium reference will
the correlation change over time
Didymium, Wavelength reference cell, NIST traceable RM-DL $ 770.00
Samarium Oxide - UV/Vis - Wavelength
Description & NIST Traceability: Samarium Oxide in perchloric acid, sealed in a quartz cell, NIST Traceable complete with
Primary Usage: Verification of wavelength accuracy in both UV/Visible, resolving complex peaks
Certified Wavelengths: 235.2, 266.7, 279.1, 280.2, 305.3, 317.6, 331.7, 344.6, 401.5, 415.2 441.2, 463.5
478.5 nm
Physical Configuration: Far UV quartz cell that has been permanently sealed by heat fusion
Samarium Oxide in a perchlorate solution is a particularly good reference for establishing
wavelength calibration because it has useful peaks from 230 to 560 nm. Many peaks have a
natural bandwidth of less than 5 nm permitting precise location of the peaks.
This offers a distinct advantage over Glass Filters, which may have natural bandwidths
greater than 30 nm.
In addition to offering wavelength calibration, the Samarium oxide can be used to check
instrument spectral bandwidth. Using peaks in the 230-240 nm or the 410-420 nm region, the
instrument capability to resolve complex peaks can be tested.
Samarium Oxide
0.9
0.8
0.7
0.6
Absorbance (A)
0.5
0.4
0.3
0.2
0.1
0
220 270 320 370 420 470 520
Wavelengths (nm)

Samarium presents a wide range of sharp resolvable peaks, be required to check or adjust your instrument. Samarium is
width (SBW) if possible and scan rate to produce a usable
of regular analysis. Periodic use of the Holmium reference will
the correlation change over time.
Samarium Oxide, Wavelength reference, NIST Traceable RM-SL $ 770.00

Rare Earth Oxide - Far UV - Wavelength Reference
Description & NIST Traceability: Rare Earth Oxide (0.05%) in sulfuric acid (0.03%) sealed in a quartz cell, NIST Traceable
Primary Usage: Verification of the Far UV wavelength scale of spectrophotometers
Certified Wavelengths: 201, 212, 223, 240, 253 nm
Physical Configuration: Filled far UV quartz cell that has been permanently heat sealed
The validation of the wavelength scale in the Far UV is difficult because of the lack of
suitable reference materials.
Starna has developed a new Rare Earth reference material which allows wavelength scale
validation down to 200 nm. The reference consists of a solution of the Rare Earth material
dissolved in an aqueous solution of very dilute sulfuric acid, which has been permanently
heat fusion sealed into a high quality quartz cuvette.
The use of the liquid filled quartz cell ensures that the optical configuration method for
quality control is exactly the same as for a normal analysis.
Each reference set is supplied with a NIST Traceable certificate, which indicates the
expected wavelength of each of five peaks and the confidence limit of the analysis.
Rare Earth Liquid
1.0000
0.9000
0.8000
0.7000
0.6000
Absorbance (A)
0.5000
0.4000
0.3000
0.2000
0.1000
0.0000
185.00 205.00 225.00 245.00 265.00 285.00 305.00
Wavelengths (nm)

This Rare Earth reference presents a wide range of resolvable Rare Earth is measured against air as a blank makes no
peaks, which are easy to compare with the wavelength difference to the certified values. It is not recommended or
indication on your spectrophotometer. The first procedure good practise to evaluate absorbance using a wavelength
should be to scan the Rare Earth reference cell over the reference.
usable range to ensure that all of the peaks can be resolved.
Adjust the slit width and scan rate to produce a usable
of regular analysis. Periodic use of the Rare Earth reference
will enable the user to build a data log of the instrument’s
spectrophotometer is within the manufacturer's tolerances for
spectral accuracy in the Far UV for use with certification and
the wavelength of your spectrophotometer. If not, a service
troubleshooting should the correlation change over time.
technician may be required to check or adjust your instrument.
Far UV Wavelength Reference, NIST traceable RM-RE $ 850.00
Metal on Quartz Near Infra Red (NIR) Absorbance/Linearity
Description & NIST Traceability: Metal on Quartz Filter for absorbance/linearity in the Near IR area of the spectrum. NIST
Traceable complete with UKAS ISO/IEC 17025 accredited certificate of calibration
Certified Wavelengths: 250.0, 360.0, 465.0, 546.1, 635.0, 1100, 1700, 2210, 2500, 2800 nm
Physical Configuration:
Metal coated quartz filter with coated side protected by an optically contacted quartz
window. The filters are mounted in protective aluminum holders that will fit a standard
instrument cell holder.
ISO Accredited: ISO/IEC 17025, ISO Guide 34 by UKAS & ISO 9001 by BSI.
A standard range of Metal on Quartz absorbance standards NIR Metal-on-Quartz ﬁlters
for the NIR area of the spectrum to facilitate full compliance 100.00
with USP requirements. 90.00
All Starna Metal on Quartz Filters have an optically 80.00
contacted quartz cover plate to protect the metalised 70.00
surface and facilitate easy cleaning. 60.00

9NQ
Transmission (%T)
5NQ
The filters allow absorbance and linearity at certified

50.00
3NQ
wavelengths across the range from 250 nm to 2800 nm

40.00
1NQ
and the relative values are shown on the table to the 30.00
right. They can be certified at wavelengths other than 20.00
those shown above, on 10.00
request. 0.00
200 700 1200 1700 2200 2700 3200
The filters are produced Wavelength (nm)
in our ISO 9001

accredited production Available Metal on Quartz NIR Filters:
facility under ISO Guide Part No. %T Transmittance Absorbance
34 as a reference NQ1 1% 2.00 A
material producer and NQ3 3% 1.52 A
calibrated in our ISO/ 1NQ 10% 1.00 A
IEC 17025 accredited 3NQ 30% 0.52 A
Calibration Laboratory. 5NQ 50% 0.30 A
RM-1NQ3NQ9NQ 9NQ 90% 0.06 A

The NIR Metal on Quartz Filters are intended for use across budget† of the certified references into consideration, if the
a broad spectrum including the NIR with a relatively flat absorbance values fall within the expected parameters† of your
transmission profile across all wavelengths. Normally three instrument then your instrument is working correctly.
representative absorption filters would be chosen for any given
It is important to confirm that Metal on Quartz Filters are
application but, the filters may be bought individually, in sets as
suitable for use with your instrument as they have a highly
shown below or any combination from the standard range.
reflective surface and some instruments are sensitive to the
Compare the spectrophotometer absorbance values to the positioning of such materials in the sample holder.
Ordering Information (Let us know what combinations you require):

Metal on Quartz NIR Reference, 30%, & Blank Holder RM-3NQ $ 1990.00
Metal on Quartz NIR Reference, 3%, 50%, & Blank Holder RM-NQ35NQ $ 3090.00
Metal on Quartz NIR Reference, 1%, 3%, 50%, Blank Holder RM-NQ1NQ35NQ $ 4220.00

Near Infrared - Wavelength Reference
Description & NIST Traceability: Quartz cell in either Transmittance or Transmittance/Transflectance format. NIST
Primary Usage: Verification of wavelength scale accuracy in the NIR spectrum
Certified Wavelengths: 990.7, 1154.9, 1446.6, 1652.3, 1693.1, 1948.0, 2060.1, 2111.0, 2248.2, 2353.3,
2373.4, 2385.4, 2537.5 nm
Physical Configuration: Filled near IR quartz cell that has been permanently heat sealed
The reference is used to validate the wavelength
scale of a NIR spectrophotometer. With 13 certified
peaks from 990 to 2537 nm that include some
complex for instrument resolution evaluation. The
reference is available in two formats:
Transmittance: - 10mm optical pathlength with two
clear windows (opposite windows to allow light to
pass through the cell).
Transmittance/Transflectance - 5mm or 10mm
optical path length, depending on orientation. In
5mm configuration, the rear window is mirror coated
to provide reflectance optical return so pathlength is
effectively 10mm ( 2 x 5mm = 10mm).
Each reference set is supplied with full certification
and traceability to NIST through SRM-2065. The
reference material is produced and certified in Transmittance/Transflectance
Transmittance
our ISO Guide 34 and ISO/IEC 17025 accredited
laboratory. The reference can also be recertified as
required.
TS5 NIR Solution
Resolvable Peaks 1.2
990.7 nm
1154.9 nm 1
1446.6 nm
1652.3 nm 0.8
1693.1 nm
Absorbance (A)
0.6
1948.0 nm
2060.1 nm 0.4
2111.0 nm
2248.2 nm 0.2
2353.3 nm
2373.4 nm 0
2385.4 nm 800 1000 1200 1400 1600 1800 2000 2200 2400 2600
2537.5 nm Wavelengths (nm)
NIR Wavelength Reference, Transmittance, NIST Traceable RM-NIR $ 1,290.00
NIR Wavelength Reference, Transmittance and Transflectance, NIST Traceable RM-NIR/T $ 1,480.00
Stray Light Reference Materials
Description & NIST Traceability: Materials with sharp cutoffs in transmission at specified wavelengths, NIST Traceable
Primary Usage: Detection of stray light in the UV and Visible
Usable Range: 200 nm to 390 nm, depending on the material
Physical Configuration: Filled far UV quartz cell that has been permanently heat fusion sealed
Stray light is an indication of light transmitted through the sample,
when there should not be light being transmitted. The presence of
more stray light than specified in the instrument operator's manual
may cause errors in your analysis.
Stray light can be a problem over any wavelength range but
the problem increases the further into the UV that instrument
measurements are taken.
The stray light reference materials are intended to determine the
presence of stray light in your instrument. Each material stops the
transmission of light below a specified wavelength. Any indication
of light transmission below the specified “cutoff” wavelength by
definition, must be stray light.
Testing for stray light is important even if the spectrophotometer is
not used below 390 nm, because it is an excellent indication of the
overall health of the instrument optics, grating and deuterium lamp. Material: Cutoff: Concentration:
Stray light determinations are run against a water blank supplied with Sodium Nitrite 390 nm 5% aqueous
all NIST traceable stray light references. The stray light calibration Potassium Iodide 260 nm 1% aqueous
certificate is measured against the water blank supplied with the Sodium Iodide 260 nm 1% aqueous
reference cell. When there are more than one stray light references Lithium Carbonate 227 nm saturated
in a set, they are all calibrated against a single stray light blank cell. Sodium Chloride 205 nm 1% aqueous
Potassium Chloride 200 nm 1.2% aqueous
Acetone 326 nm Spectroscopy grade

The procedure for using the stray light references is similar below the cutoff wavelength will be stray light. If the amount
for all materials. Set your spectrophotometer’s wavelength 20 of stray light is greater than the specification given in your
nm above the cutoff for the stray light reference that you are instrument manual, a service technician may be required to
using (for Potassium Iodide you would start at 280 nm). Insert investigate and correct the problem.
the stray light reference cell in the measurement cell holder
Periodically rescan with the same instrument configuration and
and the stray light blank cell in the blank cell holder of your
compare the results. Over time you will have a data trail for
instrument. Scan down into the UV to the lowest wavelength
your instrument which will make the detection and correction of
that your instrument manual specifies. Any light transmitted
any problems relating to stray light much more reliable.
Description Part Number, NIST Traceable Price per set
Sodium Nitrite & Blank Cell RM-SN $ 810.00
Potassium Iodide & Blank Cell RM-KI $ 810.00
Sodium Iodide & Blank Cell RM-SI $ 810.00
Lithium Carbonate & Blank Cell RM-LC $ 810.00
Potassium Chloride & Blank Cell RM-KC $ 810.00
Sodium Chloride & Blank Cell RM-SC $ 810.00
Acetone RM-AC $ 780.00

Stray Light Reference Materials
Effects of Stray Light Linearity on Spectrophotometer

5.0
4.5 Monochromator
4.0 Stray-light at 5.5A

(0.0003 %T) level
Theoretical Absorbance (A)

(0.02 %T) level
(0.03 %T) level
2.5
Stray-light at 3.0A
2.0 (0.10%T) level

Stray-light at 2.8A
1.5 (0.16 %T) level
Stray-light at 2.5A
1.0 (0.32 %T) level
Stray-light at 2A
0.5 (1.00 %T) level
0.0
0 1 2 3 4 5
Measured Absorbance (A)
Stray Light Cut-off Spectra

Sodium
4.00
Nitrite
3.50 Acetone
3.00
Sodium
Iodide
2.50
Potassium
Absorbance (A)
2.00 Iodide
Lithium
1.50
Carbonate
1.00 Potassium
Chloride
0.50
Sodium
Chloride
0.00
5
0
19
21
22
24
25
27
28
30
31
33
34
36
37
39
40
42
Wavelengths (nm)
Benzene Vapor for Spectral Resolution
Description: 0.1ml Benzene in a vapor state sealed in a quartz cell
Primary Usage: Test the resolution at various bandwidths of grating based instruments in the UV
Usable Range: 270 nm to 230 nm
Physical Configuration: Far UV quartz cell that has been permanently heat fusion sealed
The ability of your spectrophotometer to resolve absorption peaks is the basis of
its theory of operation. If absorption peaks cannot be resolved adequately, then
interfering peaks will be integrated with the correct peaks, thus giving an inaccurate
measurement.
Benzene vapor is an effective material for the detection of a grating based
spectrophotometer’s resolving power, as it has a great number of close but distinct
absorption peaks.
Benzene vapor may not work well with a photodiode array spectrophotometer.
Benzene Vapour Spectra at various

Spectral Bandwidths (SBWs) - offset for clarity
2.5
Absorbance (A)
2
0.1 nm SBW
1.5 0.2 nm SBW
1 0.5 nm SBW
1.0 nm SBW
0.5
0
252.00 254.00 256.00 258.00 260.00 262.00
Wavelength (nm)

Set your spectrophotometer to a Spectral Band Width the resolution. Make a note of the settings in your quality
(SBW) and a scan rate and note the settings. Scan from procedures.
240 to 265 nm and compare the peaks to the two charts
Each time that the scan is repeated you can compare the new
and determine what changes need to be made to the setting
scan with previous scans to check for any variance. If your
of your spectrophotometer to improve the resolution of the
instrument needs service because of a drop in resolution, the
scan. Continue to alter the slit width until you have optimized
historical data will greatly assist the service technician.
Description Part Number Price
Instrument Resolution reference cell, Benzene Vapor RM-BZ $ 450.00

Ultraviolet Bandwidth- Toluene in Hexane
Description & NIST Traceability: Quartz cell filled with Toluene in Hexane and permanently heat fusion sealed, NIST
traceable complete with UKAS ISO/IEC 17025 accredited certificate of calibration
Primary Usage: Determination of bandwidth and resolution in the UV region
Usable Range: 267.0 nm and 268.7 nm
Physical Configuration: Far UV quartz cells that have been permanently sealed by heat fusion
The Spectral Bandwidth (SBW)
of a spectrophotometer is the
basis of establishing its ability Toulene in Hexane
to resolve spectral features
separated by small differences 0.45 SBW 05 nm
in wavelength. If a sample to
be measured has an absorption 0.40 SBW 10 nm
peak at 257.4 nm and there is 0.35 SBW 15 nm

Absorbance
an interfering peak at 260.0 nm

then it should be established 0.30 SBW 17 nm
whether or the spectrophotometer
is able to resolve the two peaks 0.25 SBW 18 nm
separately or “mixes” the two SBW 20 nm

absorption peaks into a single 0.20
peak, leading to erroneous 0.15
SBW 30 nm
results.
265 266 267 268 269 270
Toluene in Hexane is used as
Wavelength (nm)
a reference for the calculation
of SBW. The ratio of the
transmittance/absorbance of the
solution when read at two peak
wavelengths (~268.7 and ~267.0
nm) relates directly to the SBW of
the instrument being assessed.
Regular use of this technique will
assure that the resolution of your
Table of Approximate Ratios
instrument is within the required
range for your work. Ratio: 2.5 2.1 1.6 1.4 1.0
SBW: 0.5 1.0 1.5 2.0 3.0
The Toluene in Hexane reference
consists of two far UV quartz
spectrophotometer cells with
the solution permanently sealed
by heat fusion in the cells. One
cell contains 0.02% Toluene in
Hexane, the other is a Hexane
only blank. The Toluene in
Hexane cell is traceable to SRM
935a which is a transmittance/
absorbance reference material.
Toluene in Hexane cell with blank, NIST traceable RM-TX $ 860.00
Neutral Density Filters
Description & NIST Traceability: Neutral Density Glass Filters for absorbance/linearity in the Visible area of the spectrum.
NIST traceable complete with UKAS ISO/IEC 17025 accredited certificate of calibration
Primary Usage: Validation of the visible of photometric scale of spectrophotometers.
Certified Wavelengths: 440.0, 465.0, 546.1, 590.0, 635.0 nm
Physical Configuration: Glass Filters mounted in anodized aluminum holder
The Starna neutral
density filter sets are
Set RM-1N2N3N
based on and traceable This set is identical to NIST SRM 930e and consists of
to the NIST sets SRM three filters and a blank holder. Approximate values are:
930e, SRM 1930 and %T Transmittance Absorbance
SRM 2930. These sets 10% 1.10 A
are used to test the 20% 0.78 A
photometric scale in 30% 0.56 A
both Transmission and
Set RM-N1N35N
Absorbance in the visible
This set is identical to NIST SRM 1930 and consists of
wavelength range.
three filters and a blank holder. Approximate values are:
The filters are made from %T Transmittance Absorbance
Schott NG-type glass, which block a known percentage of 1% 2.10 A
the light passing through it. We calibrate the glass type and 3% 1.60 A
thickness of the filters to produce known transmittance and
50% 0.30 A
absorbance values. The approximate values are shown on
the chart below. Each NIST traceable filter is individually Set RM-D1D39N
tested and certified. This set is identical to NIST SRM 2930 and consists of
three filters and a blank holder. Approximate values are:
The neutral density filters are mounted in a black anodised
%T Transmittance Absorbance
aluminum holder, 12.5 x 12.5 x 45 mm, which is fully
compatible with all standard spectrophotometer cell holders. 0.1% 3.00 A
The filters have been shown most efficient in instruments 0.3% 2.50 A
with an effective bandwidth of 2.2 nm to 6.5 nm. 92% 0.04 A

Set your spectrophotometer in either absorbance or These readings from your instrument may also be used to plot
transmission mode at 440.0 nm. Using the empty filter holder a graph of the instrument linearity.
(blank) zero the instrument. Insert each filter in turn and note
The user may also build a data log of the instrument’s spectral
the value. Set your instrument to each successive wavelength
photometric accuracy. This can be used for certification and
and repeat the procedure. The value that you read should be
troubleshooting should the correlation change over time. If
close to the measured certificate value listed for that filter.
there is any discrepancy in the readings, a service technician
Compare the spectrophotometer absorbance values to the can use the data collected to diagnose and correct any
certified absorbance values. Taking the expanded uncertainty problems with your instrument.
absorbance values fall within the expected parameters† of your
Neutral Density Filter set, 10, 20, 30 %T, & Blank Holder RM-1N2N3N $ 1190.00
Neutral Density Filter set, 1, 3, 50 %T, & Blank Holder RM-N1N35N $ 1190.00
Neutral Density Filter set, 0.1, 0.3, 92 %T, & Blank Holder RM-D1D39N $ 1190.00

Visible References - Neutral Density Filters
Neutral Density Filters
4
3.5
3
D1
D3
2.5
Absorbance (A)
N1
N3
2
1N
2N
1.5
3N
5N
1 9N
0.5
0
400 450 500 550 600 650 700
Wavelengths (nm)
Neutral Density Filter Transmittance

100.0000
10.0000
9N
5N
% Transmittance (%T)
3N
2N
1.0000
1N
N3
N1
D3
D1
0.1000
0.0100
400 450 500 550 600 650 700
Wavelengths (nm)
Glass Wavelength Filter - Holmium, Didymium
Description & Traceability: Holmium glass filter and Didymium glass filter, NIST traceable complete with UKAS ISO/
IEC 17025 accredited certificate of calibration
Primary Usage: Verification of wavelength scale accuracy in both UV and Visible
Usable Range: 241 nm to 640 nm (Holmium for UV/Vis). 430 nm to 890 nm (Didymium for Visible)
Spectral Band Width: The certificate indicates the above wavelength measured at 0.1, 0.25, 0.50, 1, 1.5, 2,
Physical Configuration: Stress free glass filter mounted in an anodized aluminium holder
Both Holmium and Didymium Holmium Glass
provide distinctive peaks that 1.8
make each suitable for use as 1.6
a wavelength standard. These 1.4
robust Starna filters are more 1.2

Absorbance (A)
suitable for some environments

1
0.8
than the liquid cells. They 0.6
are mounted in an anodized 0.4
aluminum housing to protect 0.2
the polished surfaces. 0
230 280 330 380 430 480 530 580 630
The Holmium and Didymium Wavelength (nm)
Glass Filters are used to Didymium Glass
ensure that the instrument
wavelength scale is within 3.5
the expected tolerances for 3

the actual wavelength being
measured. 2.5
Absorbance (A)
1.5
0.5
0
300 400 500 600 700 800 900

Wavelengths (nm)
Holmium and Didymium filters present a wide range of peaks within the manufacturer's tolerances of the wavelength readout.
that can be resolved. The number of peaks resolved will If not, a service technician may be required to check or adjust
depend on the spectral bandwidth used. The wavelength peaks your instrument. The filters should be measured against an air
can then be used to compare the wavelength indicated by your blank.
spectrophotometer to the known peak.
Spectra should be checked regularly, at a minimum in the
The initial procedure should be to scan the filter over the area of regular analysis. Periodic use of the Holmium or
usable range to identify all the peaks that can be resolved Didymium reference will enable the user to build a data log of
at the specified SBW of the instrument. Adjust the slit width the instrument’s spectral accuracy for use with certification and
(SBW)and scan rate to produce a usable spectra. Check each troubleshooting should the correlation change over time.
peak to ensure that the reading on your spectrophotometer is
Holmium Glass, Wavelength reference filter, NIST traceable RM-HG $ 350.00
Didymium Glass, Wavelength reference filter, NIST traceable RM-DG $ 480.00

RM-1N3N5DHG - Photometric Scale / Wavelength
Description & Traceability: Neutral Density Filter Set plus Holmium Glass Filter, NIST traceable complete with
Primary Usage: Validation of the visible of photometric scale and the UV/Vis wavelength scale
Usable Range: 440.0 to 635.0 nm (1N3N5D); 241 to 640 nm (Holmium for UV/Vis)
Physical Configuration: Glass Filters mounted in anodized aluminum holder
The elements of this set include
neutral density filters that are used
to test the photometric scale in both Set RM-1N3N5DHG
Transmission and Absorbance in the
visible wavelength range. The filters This set consists of 3 neutral density filters,
are made from Schott NG-type glass a blank holder and a Holmium Glass filter.
which absorbs a known percentage of
the light passing through it. Neutral Density filter's Approximate values
are:
Adding the Holmium glass filter to
%T Transmittance Absorbance
the set facilitates validation of the
10% 1.00 A
wavelength scale by utilizing the
30% 0.50 A
distinctive peaks of the Holmium filter.
52% 0.25 A
The thickness of the filters is
calculated to produce known The Holmium Glass filter is certified at 11
transmission and absorption values. The approximate values are shown on distinctive peaks ranging from 241 to 640.
the chart. Each NIST traceable filter is individually tested and certified. The The seven certified Spectral Band Widths
neutral density and Holmium filters are mounted in black anodized aluminum (SBW) measured are 0.10, 0.25, 0.50,
holders, 12.5 x 12.5 x 45 mm, which is fully compatible with all standard 1.00, 1.50, 2.00, and 3.00 nm.
spectrophotometer cell holders. The filters have been shown to be effective
in instruments with bandwidths of 2.2 nm to 6.5 nm.

Set your spectrophotometer in either absorbance or Holmium wavelength reference filter over the usable range to
transmission at 440.0 nm. Using the empty filter holder (blank) ensure that all of the peaks can be resolved.
zero the instrument. Insert each filter in turn and note the
Adjust the slit width (SBW) and scan rate to produce a usable
value. Set your instrument to each successive wavelength and
repeat the procedure.
spectrophotometer is within the manufacturer's tolerances for
Compare the spectrophotometer absorbance values to the the wavelength of your spectrophotometer.
of regular analysis. Periodic use of the Neutral Density and
absorbance values fall within the expected parameters† of your
Holmium references will enable the user to build a data log of
instrument then your instrument is working correctly. These
the instrument’s spectral accuracy for use with validation and
readings can be plotted to check the instrument linearity.
troubleshooting should the correlation change over time
For wavelength validation the procedure should be to scan the
Neutral Density & Wavelength Filter set, NIST Traceable RM-1N3N5DHG $ 1650.00
Microplate Reader Visible Reference Plate
Description: 96 well microplate validation filters for wavelength and absorbance scales, NIST
Primary Usage: Validation of the visible of photometric scale of 96 well microplate readers.
Certified Wavelengths: Wavelength: Neutral Density - 440.0, 465.0, 546.1, 590.0, 635.0 nm.
Holmium - 360, 418, 445, 453, 460, 536, 637 nm, Absorbance scale: 440 nm to 635 nm
Physical Configuration: Glass Filters mounted in 96 well format anodized aluminum holder
Our 96 well microplate reader filter sets are based on and traceable
to the NIST sets SRM 930e, SRM 1930 and NIST 2034. This
microplate is used to test the photometric scale in both Transmission
and Absorbance and wavelength accuracy in the visible wavelength
range.
The absorbance filters are made from Schott NG-type glass, which
absorbs a known percentage of the light passing through it. The
thickness of the particular glass type used is calculated to produce
filters of approximate transmittance/absorbance values, these
approximate values are shown on the chart below. Each NIST
traceable filter is individually tested and certified. The wavelength
filter is made of Holmium glass and is effective across the visible
range. The filters have been shown to be effective in all microplate
readers on the market.
Specifications:
Certified Wavelengths:
Neutral Density Absorbance Filters:
440.0 nm 465.0 nm 546.1 nm 590.0 nm 635.0 nm
Holmium Wavelength Filter (approximate wavelengths):
637 nm 536 nm 460 nm 453 nm 445 nm 418 nm 360 nm
Filters in reference plate:

Configuration of Reference Plate:
Well Positions Filter Type Filter Values
C2 - H2 Air Blank
C4 - H4 Neutral Density 30%T (0.5 Absorbance)
C12 - H12 Holmium Peaks from 360 to 637 nm
Description Part Number, NIST Traceable Price per set
96 Well Microplate Reader Validation Plate RMMR-1N3NN1N3HG $ 2,250.00

Cuvette to 96 Well Microplate Adaptor
Description: A patented adaptor plate (Patent Number US8115922), which allows the use of standard
cuvettes in the validation of a 96 well microplate reader.
Primary Usage: Performance validation of your microplate reader with Starna Reference sets
Usable Cuvettes: Standard rectangular cuvettes and filters.
Physical Appearance: Injection molded polymer tray with slots for 8 cuvettes
Compatible Readers: The adaptor is compatible with all 96 well plate readers
Hole alignment of the CuvettePlate conforms to the international
standard and precise location of the wells in a 96 well microplate.
Slots precisely align the cuvettes over the available holes through
the bottom plate. A small ridge in the center of the CuvettePlate
slightly tilts the cuvettes so that the bubble in a liquid sample does
not interfere with the light beam of the microplate reader.
Validating your microplate reader with liquid filled cuvettes

is easy with the CuvettePlate. Place your NIST traceable
standards in the CuvettePlate and they will be aligned into
the format for your reader and held so that the bubble in the
cuvettes will not interfere with the analysis. You can use any of
the Starna validation references as well as the NIST SRM-
2034 Holmium.
Microplate readers can be validated using exactly the same NIST

traceable filters either liquid or for instance Neutral Density Filters
such as our Part Number RM-1N2N3N or NIST SRM-930), that
you may already be using to calibrate other sectrophotometers in
your laboratory.
Under these circumstances it is not necessary to purchase
extra reference materials and allows the laboratory to cross
validate and make comparisons between different types of
spectrophotometer instruments.
CuvettePlate, 96 well microplate adaptor SCP-96-8R $ 99.00
260/280 nm Validation Reference for DNA and RNA
Description: Standard solution which gives a stable 260/280 nm ratio of 1.8 to 2.0, NIST Traceable
Primary Usage: Control the quality of DNA/RNA purity analysis
Certified Wavelengths: 260 nm and 280 nm
Physical Configuration: Far UV quartz cell that has been permanently heat fusion sealed or vials with
a crimp cap or screw cap
When running DNA or RNA purity analysis on your and the confidence limit of the ratio. The validation analysis is
spectrophotometer, it is important to ensure that your performed by our ISO/IEC 17025 accredited laboratory and is
instrument is working correctly. Our DNA/RNA validation traceable to NIST.
standard in a permanently sealed quartz cell or vial (crimp
The vials with caps (both crimp cap and screw cap) reseal to
or screw cap) contains a stable solution, which mimics the
maintain the integrity of the DNACON. This format allows for
260/280 nm ratio of DNA and RNA. The volume of each vial is
the removal of the DNACON by pipet or syringe. The vials are
1.5 ml.
available in concentrations of 5x and 10x. The number of vials
The RM-DNA (permanently sealed reference) is supplied with a and the concentrations are listed below.
certificate which lists the expected 260/280 nm ratio of the cell

The importance of this reference is that it allows you to validate Generally, the 5x concentration is suitable for the Nanodrop
your work in real time. When measuring unknown samples you instrument while the 10x concentration is suitable for micro
can assure that your instrument is working correctly by reading drop systems such as Jenway/Bibby.
the validation standard both before, during and after each run
of unknowns.
Additionally, at 260 nm, results are approximately as follows:
If you are using a spectrophotometer which automatically
calculates the 260/280 ratio, then all that you need to
Light Path 5X Concentration 10X Concentration
do is place the DNA/RNA validation reference in your
spectrophotometer and run the instrument's routine for the 0.2mm 0.1 A 0.2 A
260/280 ratio. If the ratio is valid when compared to the
0.5mm 0.25 A 0.5 A
certificate then you can be assured that your instrument is
calculating the ratio correctly. In a spectrophotometer without 1mm 0.5 A 1A
an automatic calculation feature, simply use the DNA/RNA
10mm 5 A 10 A
validation reference as you would your other samples to
validate your analytical procedure.
Permanently heat fusion sealed into a cuvette RM-DNA $ 990.00
5X concentration, 1 Crimp Cap Vial DNACON5X-CC1 $ 180.00

5X concentration, 2 Crimp Cap Vials DNACON5X-CC2 $ 340.00
5X concentration, 1 Screw Cap Vial DNACON5X-SC1 $ 180.00
5X concentration, 2 Screw Cap Vials DNACON5X-SC2 $ 340.00
10X concentration, 1 Crimp Cap Vial DNACON10X-CC1 $ 180.00

10X concentration, 1 Screw Cap Vial DNACON10X-SC1 $ 180.00

260/280 nm Validation Reference for DNA and RNA
RM-DNA DNACON 1.5 ml Vials
Screw Cap Crimp Cap
Typical Uncorrected Absorbance Values:
260 nm 0.765A
280 nm 0.405A
Ratio: 1.89
DNACON 260-280 Solution

1.2
0.8
Absorbance (A)
0.6
0.4
0,2
220 240 260 280 300 320 340

Wavelengths (nm)
Quinine Sulfate Fluorescence Reference
Description & NIST Traceability: A solution of 1mg/L Quinine Sulfate in 0.105 mol/L Perchloric Acid. The
reference material is permanently heat fusion sealed in quartz fluorometer cells, NIST
Traceable complete with UKAS ISO/IEC 17025 accredited certificate of calibration.
Primary Usage: Calibration and evaluation of methods used on a fluorescence spectrophotometer.
Usable Range: 375 nm to 675 nm
Physical Configuration: Far UV quartz fluorescence cell that has been permanently heat fusion sealed. The
reference is also available with a blank if required.
NIST SRM 936a is supplied as a crystalline material and is made into a solution as described
in the Certificate of Analysis supplied with NIST SRM 936a. Starna then fills a high quality far
UV quartz fluorometer cell with the SRM solution and permanently heat fusion seals the cell.
The reference is designed for multiple use over time and for inter instrument comparisons.
Quinine Sulfate references can also be supplied with a perchloric acid blank.
Where instrument linearity needs to be established, sets with different concentrations of
Quinine Sulphate and a blank are available in the following concentrations, 0.25, 0.5, 0.75 &
1.0 mg/l.
The Starna reference set is supplied with a Certificate of Validation by our Calibration
Laboratory which is accredited by UKAS under ISO 9001, ISO/IEC 17025, and ISO Guide
34. The certificate is valid for a period of two years.
RM-QS
Corrected molecular emission spectrum

Quinine Sulfate Linearity
Quinine Sulphate
250.000
1.000
Linearity Regression
0.900
200.000
0.800
Coefficient 0.999 0.700
Absorbance (A)
Relative Intensity Units (RIU)
150.000
0.600
0.500
100.000 0.400
0.300
50.000 0.200
0.100
0.000
0.000 375.0 425.0 475.0 525.0 575.0 625.0 675.0
0.00 0.20 0.40 0.60 0.80 1.00 1.20
Wavelengths (nm)
Quinine Sulfate (mg/l)
Quinine Sulfate Reference (1 concentration 1.0 mg/l, without blank) RM-QS $ 1050.00
Quinine Sulfate Reference (1 concentration 1.0 mg/l & blank) RM-QS00 $ 1400.00
Quinine Sulfate Reference (2 concentrations, & blank) RM-2QS00 $ 2190.00

Raman Reference, very high signal to noise ratio
Description: Ultra-high pure water, permanently heat fusion sealed in a high quality
quartz fluorometer cell.
Primary Usage: Evaluation of sensitivity in the qualification of fluorescence spectrophotometers
specifically the instrument Signal to Noise Ratio near the detection limits using the Raman
band emission at specific wavelengths.
Usable Range: 250 to 600 nm
Physical Configuration: Far UV quartz fluorescence cell that has been permanently heat fusion sealed.
ISO/IEC Accreditation: Produced and manufactured under ISO/IEC 17025, ISO Guide 34 by UKAS, protocols
With years of development, Starna has Molecular Emission Spectrum
perfected the technique of producing
Raman Water Sensitivity
Ultra Pure water and permanently heat
Excitation: 350 nm Emission Peak:
Raman398
Waternm
Sensitivity
fusion sealing the water into a high
quality Far UV quartz fluorometer cell.
The reference can be used multiple 700
times on multiple instruments because
the material does not deteriorate.
600
500
The Starna reference with a Certificate
700
400
of Validation by our reference lab which 600
is accredited by UKAS under ISO 9001,

300
500
ISO/IEC 17025, and ISO Guide 34. 400

200
100
300
0
200
372 382 392 402 412 422 432 442 452 462
100
Wavelength (nm)
0
372 382 392 402 412 422 432 442 452 462
Wavelength (nm)
Molecular Emission Spectrum

Excitation: 500 nm Emission Peak: 600.4 nm
60
50
60 40
50 30
40 20
30 10
20 0
560 580 600 620 640 660
10
Wavelength (nm)
0
560 580 600 620 640 660
Wavelength (nm)
Ultra-pure Water Reference RM-H2O $ 1050.00
Sets Recommended For Instrument Qualification
To make instrument Qualification easier, we have assembled reference
materials into sets which allow you to meet specific verification requirements.
It is more cost effective to purchase a group of references in a set instead of
purchasing several references individually.
The following list of sets will allow you to meet the qualifications of the
European Pharmacopoeia, the United States Pharmacopeia and sets that
will satisfy the requirements of both the EP and USP.
European Pharmacopoeia:
Part Number: RM-0660HLKCTX NIST Traceable Price $ 3,090.00
This set is used to meet European Pharmacopoeia requirements and contains:

Potassium Dichromate blank Photometric Accuracy 235 nm to 350 nm
Potassium Dichromate 60 mg/L Photometric Accuracy 235 nm to 350 nm
Potassium Dichromate 600 mg/L Photometric Accuracy 430 nm
Holmium Oxide Wavelength Accuracy 240 nm to 650 nm
Stray Light blank Stray Light 200 nm to 400 nm
Potassium Chloride, blank Stray Light 198 nm
Toluene in Hexane Resolution 267 nm to 268.7 nm
European Pharmacopoeia and USP:

Part Number: RM-0660HLKCSITX NIST Traceable Price: $ 3,560.00
This set meets European Pharmacopoeia requirements as well as the United States Pharmacopeia requirements and contains:
Potassium Dichromate 600mg/L Photometric Accuracy 430 nm
Potassium Chloride Stray Light 198 nm
Sodium Iodide Stray Light 220 nm
Professional & Support Services Set for all Pharmacopoeias and USP:
Part Number: RM-0660HLKCSISNTX NIST Traceable Price: $ 4,030.00
This set meets European Pharmacopoeia requirements as well as the requirements of the United States Pharmacopeia.
Potassium Dichromate 600 mg/L Photometric Accuracy 430 nm
Potassium Chloride Stray Light 198 nm
Sodium Iodide Stray Light 220 nm
Sodium Nitrate Stray Light 340 nm

Sets Recommended for Instrument Qualification
United States Pharmacopeia set:
Part Number: RM-06HLKI NIST Traceable Price: $ 1,990.00
This set is primarily used in North America due to it's similarity to NIST SRMs and contains:
Holmium Perchloric Wavelength Accuracy 240 nm to 650 nm
Potassium Iodide Stray Light 260 nm
United States Pharmacopeia set with Didymium:

Part Number: RM-06DLKI NIST Traceable Price: $ 2,220.00
This set is used where wavelength verification is required at higher visible wavelengths and contains:
Didymium Perchloric Wavelength Accuracy 290 nm to 870 nm
Potassium Iodide Stray Light 260 nm
United States Pharmacopeia set with linearity:

Part Number: RM-020610HLKI NIST Traceable Price: $ 2,670.00
This set is used where absorbance linearity in the UV is required to be validated as well as the wavelength and stray light
requirements of the USP. The set contains:
Potassium Dichromate 20, 60, 100 mg/L Photometric Accuracy 235 nm to 350 nm
Holmium Perchloric Wavelength Accuracy 240 nm to 650 nm
Potassium Iodide, blank Stray Light 260 nm
UV Absorbance/Transmittance Scale & Linearity validation:

Part Number: RM-0204060810 NIST Traceable Price: $ 2130.00
With five concentrations of Potassium Dichromate, used to test the photometric scale and linearity between 0.095A and 1.5A.
Potassium Dichromate Blank
Potassium Dichromate 20, 40, 60, 80, 100 mg/L Photometric Accuracy 235 nm to 350 nm
Part Number: RM-121416182224 NIST Traceable Price: $ 2,450.00
With six concentrations of Potassium Dichromate, used to test the photometric scale and linearity between 1.5A and 3.3A.
Potassium Dichromate 120, 140, 160, 180, 220 & 240 mg/L Photometric Accuracy 235 nm to 350 nm
Part Number: RM-040812162024 NIST Traceable Price: $ 2,450.00
With six concentrations of Potassium Dichromate used to test the photometric scale and linearity between 0.19A to 3.3A.
Potassium Dichromate 40, 80, 120, 160, 200 & 240 mg/L Photometric Accuracy 235 nm to 350 nm
Product Summary and Price List
Description: Part No. Price US$ Description: Part No. Price US$
Photometric Scale Verification UV SBW Bandwidth Calibration
Potassium Dichromate Toluene in Hexane RM-TX $ 860.00
20 mg/L RM-02 $ 830.00
Validation of 260/280 ratio
40 mg/L RM-04 $ 830.00
DNACON Sealed Cell RM-DNA $ 990.00
60 mg/L RM-06 $ 830.00
Crimp Cap Vial
80 mg/L RM-08 $ 830.00
1 Vial 5X concentration DNACON5X-CC1 $ 180.00
100 mg/L RM-10 $ 830.00
2 Vials 5X concentration DNACON5X-CC2 $ 340.00
120 mg/L RM-12 $ 830.00
140 mg/L RM-14 $ 830.00
1 Vial 10X concentration DNACON10X-CC1 $ 180.00
160 mg/L RM-16 $ 830.00
180 mg/L RM-18 $ 830.00
200 mg/L RM-20 $ 830.00
Screw Cap Vial
220 mg/L RM-22 $ 830.00
1 Vial 5X concentration DNACON5X-SC1 $ 180.00
240 mg/L RM-24 $ 830.00
2 Vials 5X concentration DNACON5X-SC2 $ 340.00
600 mg/L RM-60 $ 830.00
4 Vials 5X concentration DNACON5X-SC4 $ 590.00
PDC 5 concentration set RM-0204060810 $ 2,130.00
1 Vial 10X concentration, DNACON10X-SC1 $ 180.00
Photometric Scale Verification UV.VIS
Absorbance scale & Linearity Visible
Metal on Quartz
Neutral Density Filter sets
1% & Blank RM-Q1 $ 1290.00
10, 20, 30%T RM-1N2N3N $ 1,190.00
3% & Blank RM-Q3 $ 1290.00
1, 3, 50%T RM-N1N35N $ 1,190.00
10% & Blank RM-1Q $ 1290.00
0.1, 0.3, 92%T RM-D1D39N $ 1,190.00
30% & Blank RM-3Q $ 1290.00
50% & Blank RM-5Q $ 1290.00 Microplate Absorbance scale
90% & Blank RM-9Q $ 1290.00 and Wavelength Accuracy Visible
3%, 50% & Blank RM-Q35Q $ 1890.00 Microplate Reference RMMR-1N3NN1N3HG $ 2,250.00
10%, 30%, 90% & Blank RM-1Q3Q9Q $ 2850.00 Microplate Cell Adaptor SCP-96-8R $ 99.00
Starna Green
25X conc & Blank RM-1SG $ 770.00 Wavelength Accuracy NIR
50X conc & Blank RM-2SG $ 770.00 NIR transmittance reference
75X conc & Blank RM-3SG $ 770.00 RM-NIR $ 1,290.00
100X conc & Blank RM-4SG $ 770.00 NIR transmittance/reflectance reference
4 conc & Blank RM-1SG2SG3SG4SG $ 1,720.00 RM-NIR/T $ 1,480.00
Far UV Photometric Scale Verification Photometric Scale Verification NIR

Nicotinic Acid Metal on Quartz
6mg/L RM-1A $ 880.00 1% & Blank RM-NQ1 $ 1290.00
12mg/L RM-2A $ 880.00 3% & Blank RM-NQ3 $ 1290.00
18mg/L RM-3A $ 880.00 10% & Blank RM-1NQ $ 1290.00
24mg/L RM-4A $ 880.00 30% & Blank RM-3NQ $ 1290.00
4 concentration set RM-1A2A3A4A $ 1,980.00 50% & Blank RM-5NQ $ 1290.00
90% & Blank RM-9NQ $ 1290.00
UV/VIS Wavelength Accuracy 3%, 50% & Blank RM-NQ35NQ $ 1890.00
Holmium Glass filter RM-HG $ 350.00 10%, 30%, 90% & Blank RM-1NQ3NQ9NQ $ 2850.00
Didymium Glass filter RM-DG $ 480.00
Holmium Oxide RM-HL $ 550.00 Fluorescent Reference
Didymium Oxide RM-DL $ 770.00 Quinine Sulfate
Samarium Oxide RM-SL $ 770.00 1 x 1.0 mg/l, RM-QS $ 1050.00
1 x1.0 mg/l & blank RM-QS00 $ 1400.00
Far UV Wavelength Accuracy 2 concentrations, & blank RM-2QS00 $ 2190.00
Rare Earth RM-RE $ 850.00 3 concentrations, & blank RM-3QS00 $ 2980.00
4 concentrations, & blank RM-4QS00 $ 3770.00
Stray Light
Sodium Iodide RM-SI $ 810.00 Raman Reference, Very High Signal to Noise Ratio
Potassium Iodide RM-KI $ 810.00 Ultra Pure Water RM-H20 $ 1050.00
Lithium Carbonate RM-LC $ 810.00
Potassium Chloride RM-KC $ 810.00
Sodium Chloride RM-SC $ 810.00
Sodium Nitrite RM-SN $ 810.00
Acetone RM-AC $ 780.00
Instrument Resolution
Benzene Vapor RM-BZ $ 450.00

Calibration Services & Certificates on USB Stick
Calibration Services Certification on USB Stick
The Starna Calibration Laboratory offers a calibration and
re-calibration service to ISO/IEC 17025, the highest level
of accreditation currently available, second only to National
Metrology Laboratories.
In addition to this calibration service being available for reference

materials manufactured by Starna it is also available for many
similar reference materials produced by third parties, including
organisations such as NIST, Stranaska, as well as a number of
other companies.
Certification Provided in USB
Starna Cells is also able to extend the service to cover a wide
Electronic Format
range of optical products within the UV/VIS/NIR range, We As a result of increased customer requests for certificates and
welcome enquiries for all types of similar product, even though associated data to be supplied in electronic format, especially
in some instances they may not have been produced in the first from those customers working in controlled environments, in
instance as certified references. 2013 Starna changed certificates to an electronic format.
Calibration Production Experience

This advancement in presentation has helped the efficiency
of Pharmaceutical companies, Instrument Manufacturers
& Accreditation History

and other customers worldwide, another example of Starna
taking the lead in reference material presentation. This
environmentally friendly approach allows greater customer
Starna has over forty-five years experience in the production flexibility in the use and circulation of Calibration Certificates,
of both Glass Filters and permanently heat fusion sealed liquid allowing data to be input directly into other databases.
Reference materials.
As part of the recalibration protocol certified references are
Calibration accreditation through UKAS to ISO/IEC17025 was measured as recieved, prior to any cleaning or other analysis.
achieved in 2001. This was followed in 2006 by Starna also Many customers request the 'as received data' to be included
achieving ISO/IEC Guide 34 as a Certified Reference Material when the sets are returned after recalibration, particularly
Producer. Calibration is carried out on any one of five fully where this is part of the customer ISO/IEC 17025 protocols.
qualified reference spectrophotometers. This data is a good "health" indicator relating to the
environment in which the references have been used and
The above accreditation, experience and accumulated data puts handled. Starna has often been able to offer advice in
Starna in a unique position with regard to Certified Reference their use and handling, which has saved customers from
Materials ranging from the Deep UV to the NIR unnecessary expense by replacing references, when it could
otherwise have been avoided.
The scope detailing specifically what the Starna accreditation
Expected Parameters and

covers, including uncertainty values, can be viewed at the
following URL on the UKAS web site.
Expanded Uncertainty Budgets

www.ukas.org/AccredationOthers/schedules/4001%20RMP%20Single.pdf
www.ukas.org/calibration/schedules/Actual/0659Calibration%20Single.pdf
The expanded uncertainty budget (k=2) is the tolerance that
Guarantees we could expect to measure in the assigned value on any one

of our reference spectrophotometers during the 2 year period
of certification.
Starna Reference Materials carry a lifetime Guarantee subject
When used to verify the performance of any given
to: the references being re-calibrated by Starna at intervals not
spectrophotometer this certificate tolerance must be added in
greater than two years; the references have not been subject to
a simple linear manner to the stated appropriate specification
thermal, optical or physical abuse.
accuracy of the instrument on the test, see following example:
Starna will replace free of charge any references that, under
Wavelength Absorbance
normal environmental conditions, move outside their expanded
uncertainty budget (k=3), within a two year period and cannot be Certificate ±0.10 nm ±0.0049 A
corrected by cleaning or any other process. Instrument ±0.30 nm ±0.0050 A
Total ±0.40 nm ±0.0099 A
Product Warranty Method of Shipment
All Starna® Certified Reference Materials (CRMs) are We usually ship via United Parcel Service and have all levels
warranted to meet the specifications defined in this brochure, of service available including Red (overnight).
when received by the customer. We offer a Lifetime If required we can also ship via Federal Express. Orders for
Guarantee on all CRMs, providing that they are not physically, delivery outside of the USA are shipped via UPS or Fedex
optically or thermally abused and that they have been using either the customer's account or by prepaying shipping
re-certified at least every two years by Starna. charges and adding them to the customer invoice.
Any CRM that changes value to outside its expanded
uncertainty budget (k=3), within a time frame of two years
or less since its last certification by Starna, will be replaced
Technical Information
free of charge. Warranty does not cover breakage. Any We have technical staff available to assist you with any
references to be returned under warranty require a Return of information that you may need for the selection of a reference
Merchandise Authorization (RMA) number. The RMA can be or its use. The best method of inquiry is via e-mail at: sales@
obtained by calling our customer service department. starnacells.com. We are usually able to answer your inquiries
within 24 hours via return e-mail.
Recertification Our hours of business are:

8:30AM to 4:30PM, USA Pacific Time
All Starna NIST Traceable References can be re-certified and
certificates issued for re-certified sets are identical in form to
the certificates issued with new sets. Calculated uncertainty
values are based on over fifteen years of Starna accredited
Delivery
data and over forty-five years production data. Delivery of new Certified Reference Materials is usually within
two weeks from receipt of order. Delivery of re-calibrated
Certificates are valid for a period of two years from date of
sets of CRMs is approximately two weeks from receipt of the
issue and re-certification is recommended at either one or two
returned set at the Atascadero facility. All reference sets are
year intervals depending on customer protocols. Please call
calibrated immediately prior to dispatch from the accredited
for current pricing and return instructions. We also recertify
Starna Calibration Laboratory.
references from NIST such as SRM 930, SRM 1930, SRM
2031 and SRM 2034 as well as other third party manufactured It is best practise to call for price and delivery verification
references. either prior to or on placement of order. Every effort is made
to keep delivery times to a minimum but, due to the certified
Terms of Sale nature of the product, we must do all that is necessary to
assure that the reference materials are accurate, stable and
Our terms of sale are Mastercard, VISA, American Express or properly documented as a priority.
net 30 days to recognized accounts. If you are unsure of your
account status, please call for verification of your account. Please call us at any time for a delivery estimate.
Mail orders: Phone Orders:

The prices published in this brochure are in US$ and are
current at the time of publication. They are subject to change
without notice. The prices and our terms of shipment are
calculated as FOB Atascadero, CA USA. We prepay shipping
Starna Cells, Inc (800) 228-4482
charges and add them to your charge card, invoice or UPS PO Box 1919 or
account. Atascadero, CA 93423 (805) 466-8855
E-Mail Address: FAX Orders:

World Wide Web:
sales@starnacells.com (805) 461-1575
www.starnacells.com
First Published 1994. Copyright January 2015 The copyright © of this brochure is held by Starna Cells, Inc

NIST Traceable UV/Vis/NIR Reference Sets: ISO/IEC-17025 Accredited ISO Guide 34

Загружено:

Сведения о документе

Оригинальное название

Авторское право

Доступные форматы

Поделиться этим документом

Поделиться или встроить документ

Параметры публикации

Этот документ был вам полезен?

Это неприемлемый материал?

Авторское право:

Доступные форматы

NIST Traceable UV/Vis/NIR Reference Sets: ISO/IEC-17025 Accredited ISO Guide 34

Загружено:

Авторское право:

Доступные форматы

NIST Traceable

UV/Vis/NIR Reference Sets

What makes Starna reference materials unique?

Construction of reference materials

UKAS ISO/IEC 17025 Accredited / NIST Traceable

Should I use NIST Traceable References?

2 Starna Cells, Inc (800) 228-4482

DNA and RNA 260/280 Ratio References

Microplate Reader References

Fluorescent & Raman References

References for use with methods described in...

Holmium Oxide Wavelength UV/Visible √ √ √ √ √ √

Suggestions for Use:

†For expanded uncertainty budget and expected parameters see page 35

4 Starna Cells, Inc (800) 228-4482

This NIST traceable set of one blank and

Cell# Containing Concentration

1.5000 100 mg/l Peaks: 257 and 350 nm

Linearity: Potassium Dichromate Linearity

Suggestions for Use:

6 Starna Cells, Inc (800) 228-4482

This NIST traceable set of one blank and

Cell# Containing Concentration

Potassium Dichromate Linearity

your spectrophotometer 2.5

Certified Wavelengths: 213 nm, 261 nm

Suggestions for Use:

†For expanded uncertainty budget and expected parameters see page 35

8 Starna Cells, Inc (800) 228-4482

This NIST traceable set of one blank and four increasing

Cell# Containing Concentration

spectrophotometer for linearity

All Starna Metal on Quartz Filters have an optically contacted

facilitate easy cleaning. This was also a feature of the original Q3

NIST SRM 2031.

range and the

Suggestions for Use:

Ordering Information (Let us know what combination you require):

10 Starna Cells, Inc (800) 228-4482

a filled and permanently heat

which allows it to be used into

the UV. 0.6

Offering sharp, stable peaks 0.4

over the range of 240 to 650 nm

to confirm that the wavelength

for the actual wavelength being

Suggestions for Use:

12 Starna Cells, Inc (800) 228-4482

Suggestions for Use:

Suggestions for Use:

14 Starna Cells, Inc (800) 228-4482

Suggestions for Use:

All Starna Metal on Quartz Filters have an optically 80.00

contacted quartz cover plate to protect the metalised 70.00

surface and facilitate easy cleaning. 60.00

The filters allow absorbance and linearity at certified

wavelengths across the range from 250 nm to 2800 nm

right. They can be certified at wavelengths other than 20.00

those shown above, on 10.00

The filters are produced Wavelength (nm)

in our ISO 9001

Suggestions for Use:

Ordering Information (Let us know what combinations you require):