Академический Документы
Профессиональный Документы
Культура Документы
Synopsis submitted to
By
Taiba Naseem
Reg. No. 2009-Gmdg -5085
Session: 2017-19
Department of Chemistry
Faculty of Science
UNIVERSITY OF AZAD JAMMU AND KASHMIR,
MUZAFFARABAD
UNIVERSITY OF AZAD JAMMU AND KASHMIR,
MUZAFFARABAD
SUPERVISORY COMMITTEE
Chairman
Department of Chemistry
Dean Director
Faculty of Sciences Advanced Studies & Research
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1. Introduction
Water is human life's most valuable asset, and drinking water is a basic human
requirement. However, we are far from global requirements; this issue will grow over time
(Hillie & Hlophe, 2007). The demand of pure water is rising as water quality, population growth,
materials, soluble inorganic compounds and toxic heavy metals from municipal, industrial and
agricultural activities. These pollutants change cleanwater's physical, chemical and biological
properties (Adams et al., 2006). It can be classified by waste sources in municipal and industrial
wastewater, where municipal waste sources are homes and commercial activities; often
containing feces and urine, but industrial wastewater sources are industrial and agricultural
(Adams et al., 2006). Wastewater contains high concentrations of microorganisms like viruses,
bacteria, protozoa, and toxic chemicals like trace elements, heavy metals, and radionuclides.
Thus, wastewater is one of the major sources of waterborne diseases, some of which are fatal,
such as typhoid and cholera. The polluted water caused the death of about 1.6 million people
Wastewater treatment is one of the most important issues today due to its toxic effects on
humans, agriculture and animals from pathogens and hazardous wastewater pollution. Treatment
protect the environment from pollution. Wastewater treatment can involve physical, chemical
and biological processes to clear water from various contaminants (Bartram, 1996: Bitton, 2005).
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Wastewater treatment is one of today's most important issues due to its toxic effects of
pathogens and hazardous wastewater pollution on humans, agriculture, and animals. Wastewater
treatment should be considered for pollution protection at the personal and governmental level of
investigations. Treating wastewater can involve physical, chemical and biological processes.
evaporation and reverse osmosis are used for treating wastewater. These techniques however
remove impurities from water without making them harmless (Anjaneyulu, 2018). Full
Graphene oxide (GO) or reduced graphene oxide (rGO) indicates significant removal
ability in the recovery of heavy metal ions (Cd2 +, Pd2 +, Hg2 +, Cr6 +, As3 +, etc.). Due to the
positive charge, these ions alternate with oxygen and negative functional groups on the GO to
improve cation capture. Tan et al. (2015) reported the successful adsorption of Cu2 +, Cd2 + and
Ni2 + on GO / PVA films. The balance is reached in a short time and the membrane is reused
more than six times. Zhang et al. (2016) studies a composite membrane for nanofiltration of GO
for use in the low layer (LbL) method of Torlon support. The film was removed with over 95%
of Ni2 +, Pd2 +, Zn2 + in an aqueous system and exhibited long-term stability in the 150-hour NF
test.
The use of ZnO for water and wastewater disinfection shows promise. It is beneficial to
address the constraints of conventional water treatment methods, most of which are DBP
formation. The physical chemical properties of ZnO contribute to the antibacterial activity.
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Nanoscale materials can exhibit different improved properties compared to what they show on
the microscale, mainly due to the increased surface area, thereby increasing the rate of chemical
reactions. Because of the small size compared to large scale, nanoparticles can enter the bacterial
cell wall, eventually leading to cell death. In addition, reactive oxygen species (ROS) can be
produced on the surface of nanomaterials, attacking the bacterial cell wall. As the surface grows,
antimicrobial activity increases. Another feature is the presence of surface disorders, such as
edges and angles, leading to the abrasive action of ZnO in bacterial cell wall destruction
Despite its brief period of growth, GO-metal oxide nanocomposites have drawn much
implemented to handle distinct kinds of organic dyes and heavy metal particles (Al Nafiey, et al.,
2017). Because of its distinctive characteristics such as elevated specific surface area and
chemical stability, researchers have paid much attention to RGO. The distinctive characteristics
of RGO coupled with other nanomaterials have been used for various prospective apps such as
energy storage, antibacterial, electrochemical detector and biosensors, gas sensors, etc.
(Karuppiah, et al., 2015). Due to their increased efficiency in removing toxic heavy metal ions
and other pollutants present in water, metal and metal-oxide functionalized graphene surfaces
have received great interest. RGO nanocomposites and metal oxides have been used to
Photocatalyst layout using low-cost and earth-abundant fabrics with simple recyclability
is an important and enduring feature for practical application solar energy conversion Xu, et al.,
2017). Rapid development of technologies and materials to purify water from pollutants over a
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long period of time. One of the main goals is to develop treatment methods that do not have a
great impact on the environment and are cheap. Photocatalysis can satisfy this application,
offering an alternative that is comparatively simple and low cost. Usually, photocatalysts are
photo-stable and non-toxic semiconductors capable of absorbing ultraviolet and visible light.
2. Literature Review
The removal of commercial nanoparticles by water treatment methods has not been fully
investigated. Zhang, et al., 2008) tested commercial metal oxide nanoparticles, Fe2O3, ZnO, NiO
and SiO2 to test their properties, dispersibility and stability in water, and to remove them by
water treatment. In addition to silica, nanoparticles are rapidly combined in tap water and are not
less than 80% of the total mass of the nanoparticles. It has been reported that the behavior of
metal oxide nanoparticles in water depends on their physical properties and their interaction with
The organic dye is in the form of ions (cationic or anionic) in water, and GO / rGO is
electrostatic interaction. However, other interactions, such as H-bonding and accumulation (Al
Nafiey, et al., 2017), did not exclude the adsorption of organic dyes in GO/rGO-based
composites. On the other hand, the negatively charged graphene oxide (GO) has several oxygen-
containing groups such as OH and COOH. Therefore, it has high adsorption capacity for basic
compounds and metal cations. On the other hand, reduced graphene oxide (rGO) has a
hydrophobic surface, which leads to high adsorption capacity of the dye by intense packing and
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hydrophobic interaction (Acharya, et al., 2018). Increased adsorption capacity can be achieved
The great dispersibility of GO in water has been studied well, while the dispersibility of
RGO in water has attracted little attention in the literature. Based on the above considerations,
we have improved the dispersibility of the less dispersible reduced graphene oxide in dispersible
RGO by functionalizing metals and metal oxides on the surface of graphene sheets (Dubey, et
al., 2015). Our research showed that the dispersibility improved with the metal and metal oxide
nanoparticle coatings on the RGO surface. In this study, the water contact angle and water
Single (Ag, Cu2O and Fe3O4) and bimetallic (Ag-Cu2O, Ag-Fe3O4 and Cu2O-Fe3O4)
and sodium sorbate are used as reducing agents and stabilizers. We evaluated the potential of
synthetic GMO composites to remove impurities from water. Since metal nanoparticles are
known to have excellent catalytic properties, the RGO complex has also been studied to reduce
materials in intermittent isotherm studies was investigated and compared with commercially
There are several possible processing techniques that use different available zinc-
containing materials as raw materials. The synthesis of ZnO can also be a large number of
scale processes. The main technical differences between the different processing methods relate
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to zinc precursors, process conditions, unit operations and production scale (Moezzi, et al.,
2012).
Various laboratory techniques for ZnO synthesis have been reported, including
precipitation, hydrothermal, solvothermal and sol-gel methods (Kolodziejczak, et al., 2014). The
size, particle size distribution, shape, surface area and distribution properties (Djurisic, et al.,
2012). However, it may be difficult to control all of the variables in ZnO synthesis, and different
methods and process variables will result in different nanoparticle shapes and sizes (Espitia, et
al., 2012).
In El Saeed et al. (El Saeed, et al., 2015) method for synthesizing ZnO by direct
deposition with Zn (NO3)2·6H2O and (NH4)2CO3 at 40AC. The precipitate was calcined at 550
morphology revealed that the ZnO nanoparticles were non-agglomerated with an average particle
size of 20 nm.
Motshekga, et al., 2015) has developed a nanocomposite in which silver and ZnO
E. coli and Gram-positive E. faecalis bacteria were used to test antibacterial activity. Both silver-
activity. However, Silver ZnO bentonite chitosan nanocomposites exhibit the best antibacterial
activity with a removal efficiency of at least 78%. It has also been suggested that the
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Adams et al. reported that ZnO nanoparticles have antibacterial activity on B. subtilis and
E. coli, the study reported that antibacterial activity is not affected by different particle sizes and
its activity is the same under dark and light activity on B. subtilis bacteria, but more activity
under light on E. coli (Adams, Lyon & Alvarez, 2006). Li et al. reported that the toxicity of ZnO
nanoparticles to E. coli increases with decreasing pH (Li2013). Premanathan, et al., reported that
the bacterial activity of ZnO NPs on Gram-positive bacteria such as S. aureus is more effective
than on Gram-negative bacteria such as E. coli (Premanathan, 2011). It reported that the bacterial
higher than that without surface activity against E. coli, S. aureus and B. subtilis (Qasem, 2013)
Sondos et al. reported that ZnO nanoparticles show good antibacterial agents. All activity against
E. coli under sunlight, while the antibacterial activity of ZnO nanoparticles increased as the
3. Justification
Both Graphene Oxide and Zinc Oxide are excellent adsorbent and plays an important role
reduced Graphene Oxide and their composites in AJK. Efficient manners to purify water using
graphene and its composites are required to explore. Moreover, it is a cost-effective adsorbent
that can play a vital role in a country's economy for water purification. It will also offer insights
into further study requirements and this technique will be used further to eliminate heavy metals
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4. Aims and Objectives
The main objective of this study is wastewater disinfection using environmentally friendly and
4. The antimicrobial activity of the synthesized rGO- ZnO will be performed against the
5. Methodology
Graphene will be Synthesized from graphite using modified Hummers method then
synthesis of graphene oxide, reduced graphene oxide by reduction process will be carried out by
treating graphene oxide with some reducing agents such as sodiumborohydrate or some other
reducing agent. For the analysis of prepared GO, we will used different characterization
techniques such as UV, FTIR, SEM and XRD.
In the literature, different methods have been used for the synthesis of rGO-ZnO
nanocomposites. In this thesis, rGO-ZnO nanocomposite will prepared by simultaneous chemical
reduction of GO and Zn(NO3)2.6H2O with sodium borohydride. Typically, to 5 mL of 1 mg/mL
GO suspension in DIW, will added 50 mg of Zn(NO3)2.6H2O.
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The resulting mixture will be sonicated for ten minutes. Then 5 mL of 0.1 M sodium borohydride
aqueous solution will add to the mixture at room temperature. A light purple precipitate will be
formed spontaneously. For separation centrifugation and washing will be performed repeatedly
with water.
Different characterization techniques (SEM, XRD and FTIR) will be used to analyze
rGO/ZnO nanocomposites. By using these nanocomposites, highly efficient reduction of 4-
nitrophenol into 4-amino phenol will be carried out. The progress of the reaction will be
monitored by using ultraviolet-visible spectroscopy. Antimicrobial activity of synthesized
composites will be performed.
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