MAJOR-PRACTICAL BSC BT

SEMESTER - III
MAJOR-PRACTICAL
BIOINSTRUMENTATION

OBJECTIVES :

CO1. To enable Students to understand about Analytical Techniques & to know about Principles and Mechanism
of Bioinstrumentation, CO2. To enable Students to describe current evidence regarding contributing factors that
affect balance control, CO3. To identify a balance-specific outcome measure and be able to describe the
reliability, validity, and clinical usefulness, CO4. To provide the Knowledge of pH great many situations, including
HOURS / WEEK - 8
of course chemical laboratory, CO5. To enable Students to explain the principles of a wide range of modern
analytical methods of separation and quantitation, CO6. To evaluate strengths and limitations of different
HOURS / SEMESTER - 120
separation and detection techniques with respect to sample properties and to specific analytical CO-CURRICULA
problems.
CIA LECTURE
R ACTIVITIES

16 8 96

UNIT I (LECTURE HOURS: 8)

BALANCE & pH METER
Balance - Analytical Balance, Double-pan Analytical Balance, Single-pan Analytical Balance, How to use the Balance,
Methods for Weighing, Maintenance.
pH & pH Meter - Sorensen’s pH Scale, Electrolytic Dissociation of Water. Acids & Bases- Dissociation of strong
electrolytes, Dissociation of Weak electrolytes. Buffers, Henderson- Hasselbalch Equation.pH Meter - Principle,
Calomel Electrodes and Glass Electrode.
UNIT II (LECTURE HOURS: 10)
APPLICATION OF BASIC INSTRUMENT
Homogenizer - Principles And Applications
Autoclave - Principles And Applications
Hot air oven - Principles And Applications
Incubator - Principles And Applications
Water Bath - Principles And Applications
Laminar air flow - Principles And Applications
BOD incubator - Principles And Applications
Centrifuges - Bench top, High speed, Ultra centrifuge-PrinciplesAnd Applications
UNIT III (LECTURE HOURS: 10)
CHROMATOGRAPHY
Paper Chromatography - Principles, Method and Application
Thinlayer Chromatography - Principles, Method and Application
Column Chromatography - Principles,Equipment,Elution Methods
Ion Exchange Chromatography - Principles, Method and Application
Affinity Chromatography - Principles, Method and Applications
High Performed Liquid Chromatography - HPLC Equipment, Method Application
Gas Chromatography - Principles, Method and Applications
UNIT IV (LECTURE HOURS: 10)
ELECTROPHORESIS
Principle - Effect of Shape and Size on Electrophoretic Mobility - Joule Heating
Gel Electrophoresis - Polyacrylamide Gel Electrophoresis Principle & Applications. SDS PAGE - Principle
&Applications. Agarose Gel Electrophoresis- Applications
UNIT V (LECTURE HOURS: 10)
SPECTROSCOPY
Colorimetry - Principle, Beer-Lamberts law, Application
Spectrophotometry, UV & Visible spectroscopy (UV-VIS) - Parts of a UV-VIS spectrophotometer, Application
Flame Photometry - Principle, Instrumentation, Applications

2018-2019 Batch RVS CAS (S)

 MAJOR-PRACTICAL BSC BT

SEMESTER - III
MAJOR-PRACTICAL
BIOINSTRUMENTATION
Nephelometry & Turbidometry - Theory, Instrumentation, Applications
UNIT VI (LECTURE HOURS: 48)
BIOINSTRUMENTATAION LAB
BIOINSTRUMENTATION PRACTICAL EXPERIMENTS - 1. Acid-Base Titration curves & pKa Value Determination 2.
Preparation of Buffers 3. Sterlization - Aim, Dry heat ,Moist heat, Gaseous chemicals, Filtration & Radiation, Result &
Discussion 4. Pure Culture Technique 5. Separation of lipids by Thin layer Chromatography- Aim, Principle, Procedure
& Result & Discussion. 6. Separation of plant pigments by Column Chromatography - Aim, Principle, Procedure &
Result & Discussion. 7. Preparation of SDS - PAGE -Aim, Principle, Procedure, Demonstration & Result & Discussion.
8. Preparation of Agarose gel electrophoresis- Aim, Principle, Procedure & Result & Discussion. 9. Estimation of
proteins by Bradford method 10. Estimation of DNA by Diphenylamine Method

Text Books :
Bioanalytical techniques | Edition:1 | IK International Publishing House Pvt Ltd | Sekhar Talluri(2009)
Bioinstrumentation | Edition:1 | MJP Publishers, | L Veerakumari (2015)

Reference Books :
Experimental Procedure in life sciences | Edition:1 | Anjanaa Book house | Dr. S. rajan AND R. Selvi Christy(2015)
Practical Biochemistry | Edition:3 | Tata McGraw Hill | David Plummer(2002)

2018-2019 Batch RVS CAS (S)