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Advances in Environmental Biology, 9(13) Special 2015, Pages: 8-13

AENSI Journals

Advances in Environmental Biology


ISSN-1995-0756 EISSN-1998-1066

Journal home page: http://www.aensiweb.com/AEB/

Microorganism as Plastic Biodegradation Agent towards Sustainable Environment


W. Naimatul Asiah W. Muhamad, Rashidi Othman, Ruhul Izzati Shaharuddin, Mohd Shah Irani
Hasni

International Islamic University Malaysia (IIUM), Kulliyyah of Architecture and Environmental Design (KAED), International Islamic
University Malaysia, Jalan Gombak, 53100 Kuala Lumpur, Malaysia

ARTICLE INFO ABSTRACT


Article history: The usage of plastic is critically in demand due to its character which can be
Received 12 March 2015 shaped into almost any desired shape and its ability to retain that shape. Unfortunately,
Accepted 28 April 2015 the wastage of those plastics leads to many environmental issues and hazards towards
Available online 6 June 2015 human health. Basically, plastics can degrade in the environment through four
mechanisms which are photodegradation, thermooxidative, hydrolytic degradation and
Keywords: microbial degradation. Microbial degradation is subject to microorganism which acts
Plastic, Microorganism, bacteria, as biodegradation agent. In this case, microorganism will secrete an active enzyme to
fungi, biodegradation, microbial break down long chain polymers. Microbial enzymes are attractive because the cost to
degradation, sustainable environment. grow and maintain them is less and they are easy to manipulate. Rhizopus delemar ,
Rhizophus arrizus and Pseudomonas sp. were among the most cited bacteria that have
successfully degraded certain type of plastics. Whereas, fungal species of Aspergillus
flavus and Penicillium funiculosum were recorded by scientist which react with certain
plastic’s substrate. The objective of this paper is to review several species of
microorganism that have been experimented by scientist and were claimed to have the
capability to degrade plastic towards eco-friendly approaches in order to sustain our
environment. In conclusion, microorganisms are very effective agent to fasten
degradation process of plastics without causing any side effect like chemical or
synthetic method does.

© 2015 AENSI Publisher All rights reserved.


To Cite This Article: W. Naimatul Asiah W. Muhamad, Rashidi Othman, Ruhul Izzati Shaharuddin, Microorganism as Plastic
Biodegradation Agent towards Sustainable Environment. Adv. Environ. Biol., 9(13), 8-13, 2015

INTRODUCTION

During the past 3-decades, plastic materials have been increasingly used in food clothing, shelter,
transportation, construction, medical, and recreation industries. Plastics are advantageous as they are strong,
light-weighted, and durable. However, they are disadvantageous as they are resistant to biodegradation,
leading to pollution, harmful to the natural environment. The successful production and marketing of
biodegradable plastics will help alleviate the problem of environmental pollution [1].
According to Plastic Europe, 2010, the annual production of plastic materials has doubled in the last
15years, being about 250 million tons in 2008. Due to plastic’s resilience against degradation and its
proliferation in industry, the issue of plastic pollution has evolved to become a threat to global ecology.
Plastic pollution arises from both terrestrial and marine sources. The continual stream of pollutant plastic is
maintained via two means: purposefully, through illegal or inappropriate dumping of domestic and
industrial refuse; and inadvertently, through poorly contained static and transported waste. Directed by the
elements, land-based plastic debris migrates to waterways, where it is further added to by the disposal or loss
borne from marine vessels and offshore petroleum platforms. Such pollution results in a number of deleterious
repercussions [2]. Obviously, soil pollution and water pollution are very threatening to our environment.
Asia alone is the world’s largest plastic consumers, accounting about 35% of the global consumption which
is followed by North America with about 26% and Western Europe about 23%. India (5%) along with Japan
(6%) comprises 11% of the world plastic consumption and about 40% of the total consumption in Asia [3].
Since Malaysia is categorized in Asia country, undoubtedly the usage of plastics is very broad, starting from
household equipment to industrial usage. But then, campaign of reduce, recycle and reuse is an alternative to

Corresponding Author: Dr Rashidi Othman. International Islamic University Malaysia (IIUM), International Institute for
Halal Research and Training (INHART) Kuliyyah of Architecture & Environmental Design
(KAED), P.O Box 10, Jalan Gombak, 53100 Kuala Lumpur.
Tel: 03-6196 5590 / 3151; E-mail: rashidi@iium.edu.my
9 W. Naimatul Asiah W. Muhamad et al., 2015
Advances in Environmental Biology, 9(13) Special 2015, Pages: 8-13

create public awareness among Malaysians regarding this issue.


In case when plastics are dumped in a field or in dumping areas, there is evidential proof that they are
causing a great change in the pH of the soil followed by disturbance in the leaching of the rain water and
moisture, making the land bare and unfertile. The biological degradation time of is very high, and it takes
thousands of years to degrade these long chain polymers into simple hydrocarbons. Latest reports confirmed
that some plastic products are mimicking human hormones (e.g., thyroxin and sex hormones), causing human
health hazards [4, 5]. There are many other diseases that might be caused by the chemical leachate of plastics.
Cancers are the most terrified cases caused by chemical reactions of plastics.

Plastics:
Plastics are defined as the polymer (Solid materials) which became mobile on heating and thus can be
cast into moulds. Plastics are non-metallic mouldable compounds and the materials made from them, can be
pushed into almost any desired shape and retain that shape [6]. They are durable and degrade very slowly.
These are highly to biodegradation, leading to pollution and harmful towards the ecosystem balance.
Chemically, plastic is a long hydrocarbon chain polymer having high molecular weight. Plastics are mainly
derived from petrochemicals which are further synthetically arranged by some chemical processes to
produce these long chain polymers [7]. Plastics being xenophobic compounds resistant to degradation
constitute about 5-8 percent of dry weight of municipal solid waste the instrumental effects of these
polymers on the environment, range from ozone depletion to the environmental toxicology of agriculture and
aquatic ecosystem [8].
In our daily life, plastics easily found in a form of straw, plastic bottle, plastic bag, detergent and toiletries
bottle, credit card, toys, bento boxes, polystyrene, pipes, food tray and many more. Since baby we are using
plastic starting from feeding bottles and others until our late age. In fact, we cannot get rid of using plastic in
our life even for once.

Classification of Plastics:
There are many types of plastics. Those plastics have been classified into 7 categories. The Society of
Plastic Industry (SPI) established a classification system in 1998 to allow consumers and recyclers to identify
different types of plastic. Manufacturers place an SPI code, a number on each plastic product, usually
moulded into the bottom. This guide provides a basic outline of the different plastic types associated with each
code number

Plastics And Microbial Biodegradation:


As a rule, widely used plastics do not naturally degrade to a large degree when released into the
environment [9, 10]. This is perhaps unsurprising, as one of the primary reasons for the popularity and
widespread application of many polymers is their exceptionally high stability and durability [9, 11]. There are
four mechanisms by which plastics degrade in the environment: photodegradation, thermooxidative
degradation, hydrolytic degradation and biodegradation by microorganisms [12] or known as microbial
biodegradation
Generally speaking, natural degradation of plastic begins with photodegradation, which leads to
thermooxidative degradation. Ultraviolet light from the sun provides the activation energy required to initiate
the incorporation of oxygen atoms into the polymer [12, 13]. This causes the plastic to become brittle and to
break into smaller and smaller pieces, until the polymer chains reach sufficiently low molecular weight to
be metabolised by microorganisms [11, 12]. These microbes either convert the carbon in the polymer chains to
carbon dioxide or incorporate it into biomolecules [9, 12]. However, this entire process is very slow, and it can
take 50 or more years for plastic to fully degrade [14].
Basically, plastics are usually long chains of carbon and hydrogen atoms. The enzymes found in living
things can perform many chemical reactions, but they generally exploit some sort of imbalance of electric
charge within a molecule to do their job. A long chain of carbons and hydrogens contains very balanced
charges along its length, making the molecule stable and difficult to change with enzymes. Most biodegradable
substances contain some mixture of carbon and atoms like oxygen, nitrogen, sulfur, and phosphorus, which
create charge imbalances that enzymes can exploit. There are some bacteria that can break down plastics. These
bacteria usually contain enzymes called oxygenases, which can add oxygen to a long carbon chain. This
destabilizes the local electric charge, and the plastic can then be broken down. The oxygenase enzymes too
often are not found, however, because they can easily destroy the molecules in the bacteria that carry them [15].
The clear zone method with agar plates is a widely used technique for screening polymer degraders and for
assessment of the degradation potential of different microorganisms towards a polymer. Agar plates containing
emulsified polymers are inoculated with microorganisms and the presence of polymer degrading
microorganisms can be confirmed by the formation of clear halo zones around the colonies. This happens
when the polymer-degrading microorganisms excrete extracellular enzymes which diffuse through the agar
10 W. Naimatul Asiah W. Muhamad et al., 2015
Advances in Environmental Biology, 9(13) Special 2015, Pages: 8-13

and degrade the polymer into water soluble materials. Using this technique, it was confirmed that PHB,
polypropiolactone (PPL) and PCL degraders are widely distributed in different environments [16,17,18].
Majority of the strains that are able to degrade PHB belong to different taxa such as Gram-positive and
Gram-negative bacteria, Streptomyces and fungi [17]. It has been reported that 39 bacterial strains of the classes
Firmicutes and Proteobacteria can degrade PHB, PCL, and PBS, but not PLA [18]. Only a few PLA degrading
microorganisms have been isolated and identified. The population of aliphatic polymer-degrading
microorganisms in different ecosystems was found to be in the following order: PHB = PCL > PBS > PLA
[16,17,18,19,20].

Table 1: Classification of plastics.


Plastic type General properties Common household uses

Polyethylene Terepthalate
 Good gas & moisture barrier properties  Mineral water, fizzy drink
 High heat resistance  Pre-prepared food trays & roasting bags
 Clear  Boil in the food pouches
 Hard  Soft drink & water bottles
 Microwave transparency  Fibre for clothing & carpet
 Solvent resistant  Some shampoo & mouthwash bottles
 Excellent moisture barrier properties  Detergent, bleach and fabric conditioner bottles
High Density Polyethylene  Excellent chemical resistance  Snack food boxes and cereal box liners
 Hard to semi-flexible &  Milk & non-carbonated drinks
strong bottle
 Soft way surface  Toy, buckets, rigid pipes, crates plant pots
 Permeable to gas  Plastic wood, garden
 HDPE films crinkle to the touch furniture

 Pigmented bottles stress  Wheeled refuse bins,


resistant compost containers

 Excellent transparency
Polyvinyl Chloride
 Hard, rigid (flexible when plasticised)
 Credit cards

 Good chemical resistance


 Carpet backing & other floor covering

 Long term stability


 Window & door frames,
guttering
 Good weathering ability
 Pipes & fittings, wire and cable sheathing
 Stable electrical properties
 Synthetic leather products
 Low gas permeability

Low Density Polyethylene  Tough & flexible


 Films, fertiliser bags, refuse sacks
 Waxy surface
 Packaging films, bubble wrap
 Soft-scratches easily
 Flexible bottles
 Good transparency
 Irrigation pipes
 Low melting point
 Thick shopping bags (clothes & produce)
 Stable electrical properties
 Wire & cable application
 Good moisture barrier
 Some bottle tops
properties
 Most bottle tops
Polypropylene
 Excellent chemical resistance  Ketchup & syrup bottles
 High melting point  Yoghurt & some margarine containers
 Hard but flexible  Potato crisp bags, biscuit wrappers
 Waxy surface  Crates, plant pots, drinking straws
 Translucent  Hinged lunch boxes,
 Strong refrigerated containers
 Fabric / carpet fibres, heavy duty bags / tarpaulins

 Clear to opaque
 Yoghurt containers, egg
Polysterine boxes
 Glassy surface
 Fast food trays
 Rigid or foamed
 Video cases
 Hard
 Vending cups and disposable cutlery
 Brittle
 Seed trays
 High clarity
 Coat hangers
 Affected by fats & solvents
 Low cost brittle toys
Other
 Nylon (PA)

 There are other polymers that have a wide range of  Acrylonitrile butadiene styrene
uses, particularly in engineering sectors. They are (ABS)
identified with the numbers 7 and OTHER (or a triangle
with numbers from 7 to 19).
 Polycarbonate (PC)
 Layered or multi-material mixed
polymers
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Advances in Environmental Biology, 9(13) Special 2015, Pages: 8-13

Biodegradation of plastics take an active part in different soil conditions according to the nature of the
bacteria, because the microorganisms responsible for the degradation different from each other and have
optimal growth of bacteria in the soil [1]. Since different species of bacteria and fungi react differently with
plastics, further study is required to assess the capability of each bacteria and fungi until to the optimal
degradation degree they can degrade those plastics.

Microorganism:
Microorganism is a microscopic living organism, which may be a single cell [21] or a multicellular
organism. The study of microorganisms is called microbiology, a subject that began with the discovery of
microorganisms in 1674 by Antonie van Leeuwenhoek, using a microscope of his own design.
Microorganisms are very diverse and include all the bacteria and archaea and almost all the protozoa. They
also include some fungi, algae, and certain animals, such as rotifers. Many macro animals and plants have
juvenile stages which are also microorganisms. Some microbiologists also classify viruses (and viroids) as
microorganisms, but others consider these as non-living [22].

Table 2: Some bacterial and fungal strains depicting the biodegradation of plastics [25].
Source Enzyme Microorganisms Plastic act as substrate Reference(s)
Fungal Glucosidases Aspergillus flavus Polycaprolactone(PCL) [23]
Unknown Penicillium funiculosum Polyhydroxybutyrate(PHB) [23]
Catalase, protease Aspergillus niger PCL [23]
Unknown Streptomyces PHB, PCL [23]
Cutinase Fusarium PCL [7]
Unknown Amycolaptosis sp. Polylactic Acid(PLA) [7]
Serine hydrolase Pestalotiopsis microspora Polyurethane [24]
Phanerochaete
Manganese peroxidase Polyethylene [7]
chrysosporium
Bacteria Lipase Rhizopus delemar PCL [23]
Unknown Firmicutes PHB, PCL, and PBS [23]
Unknown Proteobacteria PHB, PCL, and PBS [23]
Polyethylene Adipate (PEA),
Lipase Rhizopus arrizus [23]
PBS, PCL
Serine hydrolase Pseudomonas stutzeri Polyhydroxyalkanoate(PHA) [7]

Based on the above table, Tokiwa [23] proof that fungal species of Aspergillus flavus, Aspergillus niger,
Penicillium funiculosum and Streptomyces can degrade various kind of plastics. In addition, bacteria that has
been stated were Rhizopus delemar, Firmicutes and Rhizopus arrizus. Other scholar shimao [7] clarified
Fusarium, Amycolaptosis sp and Phanerochaete chrysosporium as fungal species and Pseudomonas stutzeri as
bacteria species to degrade respective plastic type. Russel [24] found out single species of fungi Pestalotiopsis
microspore successfully degrade polyethylene.
Recent research suggests that there have been a notable number of microorganisms (especially some
bacteria and fungi) which have the capacity to degrade these synthetic polymers in much faster way in
comparison to the natural method by using some exoenzymes under stress conditions. The enzyme lipases from
Rhizopus arrhizus, Rhizopus delemar, Achromobacter sp., and Candida cylindracea showed activities on
polyethylene adipate (PEA) and poly(ε-caprolactone) (PCL) [26, 27]. Enzymatic degradation of PCL by
Aspergillus flavus and Penicillium funiculosum showed that faster degradation was observed in the amorphous
region [28]. Moreover, a novel poly(3-hydroxybutyrate) (PHB) depolymerase from a thermophilic Streptomyces
sp. was also capable of degrading poly(β-propiolactone) (PPL) [29]. In comparative studies, the
biodegradability of three polyalkylene succinate [polyethylene succinate (PES), polybutylene succinate (PBS),
and polypropylene succinate (PPS)] with the same molecular weight was investigated using Rhizopus
delemar lipase. PPS with low melting temperature (Tm, 43 –52 °C) had the highest biodegradation rate
followed by PES, owing to the lower crystallinity of PPS compared to PES and PBS [30].

Table 3: List of microbial strains and the types of plastic which they degrade [15].
Plastic Microorganism Reference (s)
Polyethylene (PET) Brevibacillus borstelensis [31]
Rhodococcus rubber [32, 33]
Pseudomonas chlororaphis [11]
Polyvinyl chloride (PVC) Pseudomonas putida AJ [34]
Ochrobactrum TD [35]
Pseudomonas fluorescens B – 22 [35]

Based on the above table, it was identified that Polyethylene (PET) was successfully degraded by
Brevibacillus borstelensis [31], Rhodococcus rubber [32, 33] and Pseudomonas chlororaphis [11]. Meanwhile,
Pseudomonas putida [34], Ochrobactrum [35] and Pseudomonas fluorescens [35] broke down the polymer
chain of Polyvinyl chloride (PVC).
12 W. Naimatul Asiah W. Muhamad et al., 2015
Advances in Environmental Biology, 9(13) Special 2015, Pages: 8-13

Conclusion:
In this millennium era, plastic usage is undeniable. It is used worldwide and its impact started to worry
the environmentalist. In nature, it takes thousands of years to degrade plastics. During those periods various
kinds of pollution and environmental hazards will occur. Most of the plastics being dumped to the landfill area
will create leachate issues afterward. Leachate will seep into soil and waterways and causing soil pollution as
well as water pollution. Other method to get rid of these plastics is by burning it. Burning plastics usually
produces some noxious gases like furans and dioxins, which are some dangerous greenhouse gases and caused
ozone layer depletion. In fact, dioxins threatened human health. Plastics are threatening our ecosystem if not
treated wisely, thus environmental friendly method should be practiced. Since plastic is the long hydrocarbon
chain polymer with high molecular height, thus an alternative attempt to reverse flow the process which
break down those long chain into simple hydrocarbon is considered as environmental friendly approaches.
This process can be done with the production of enzymes by certain microorganism that can degrade long
chain polymers of plastics. On the other hand, microorganism uses polymer as carbon and their energy
source. The process is known as microbial degradation. Indeed it is a symbiosis process. Rhizophus delemar,
R. arrizus, Pseudomonas stutzeri, P. chlororaphis, P. fluorescens, P. putida, Firmicutes and Proteobacteria
were among the most cited bacteria claim by several scientist as biodegradation mechanism for certain type of
plastics. Whereas, fungal species of Aspergillus niger, A. flavus and Penicillium peniculosum have indicated
degradation process of plastic while experimented by some scholar as microbial biodegradation agent.
Further exploration of different microorganism species will broaden the opportunity to recover plastics
issues towards sustainable environment for the next generation.

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