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Yongjiao Sun, Lingyun Jia, Zhanbo Huang, Jing Wang, Jincai Lu & Jing Li
To cite this article: Yongjiao Sun, Lingyun Jia, Zhanbo Huang, Jing Wang, Jincai Lu & Jing
Li (2017) Hepatoprotective effect against CCl4-induced acute liver damage in mice and
High-performance liquid chromatography mass spectrometric method for analysis of the
constituents of extract of Rubus crataegifolius, Natural Product Research, 31:22, 2695-2699, DOI:
10.1080/14786419.2017.1292264
Download by: [UNIVERSITY OF ADELAIDE LIBRARIES] Date: 13 October 2017, At: 01:22
Natural Product Research, 2017
VOL. 31, NO. 22, 2695–2699
https://doi.org/10.1080/14786419.2017.1292264
SHORT COMMUNICATION
Yongjiao Suna, Lingyun Jiaa, Zhanbo Huangb, Jing Wanga, Jincai Lua and Jing Lia
a
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, China;
b
School of Biological Medicine and Chemical Engineering, Liaoning Institute of Science and Technology,
Benxi, PR China
1. Introduction
Hepatic disease is one of the most common diseases to affect human health. Carbon tetra-
chloride (CCl4) is an extensively used hepatotoxic agent in preclinical animal studies. (Han
et al. 2016) Rubus crataegifolius is also known as Niu diedu, Rosaceae Rubus, widely distributed
in north-east, Inner Mongolia, Hebei, Shanxi, Henan and other provinces (regions) of China.
Its roots are always as traditional Chinese medicine. Root portion has dispelling wind,
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Figure 1. Effects of all extracts on ALT activities in serum of mice with CCl4- induced liver injury.
Notes: Data were expressed as the mean ± SD (n = 8).(### p < 0.001,## p < 0.01,compared with the normal group;
***p < 0.001,**p < 0.01,*p < 0.05,compared with the model group).
Figure 2. Effects of all extracts on AST activities in serum of mice with CCl4- induced liver injury.
Notes: Data were expressed as the mean ± SD (n=8).(### p < 0.001,## p < 0.01,compared with the normal group;
***p < 0.001,**p < 0.01,*p < 0.05,compared with the model group).
NATURAL PRODUCT RESEARCH 2697
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Figure 3. Effects of all extracts on SOD activities in serum of mice with CCl4- induced liver injury.
Notes: Data were expressed as the mean ± SD (n=8).(### p < 0.001,## p < 0.01,compared with the normal group;
***p < 0.001,**p < 0.01,*p < 0.05,compared with the model group).
Figure 4. Effects of all extracts on GSH activities in serum of mice with CCl4- induced liver injury.
Notes: Data were expressed as the mean ± SD (n=8).(### p < 0.001,## p < 0.01,compared with the normal group;
***p < 0.001,**p < 0.01,*p < 0.05,compared with the model group).
removing dampness and other functions, for the treatment of chronic hepatitis, rheumatoid
arthritis. Modern pharmacological studies have shown that its root has a variety of pharma-
cological effects, such as anti-organisations peroxide, anti-tumour, anti-inflammatory and
antibacterial.
2698 Y. SUN ET AL.
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Figure 5. Effects of all extracts on MDA activities in serum of mice with CCl4- induced liver injury.
Notes: Data were expressed as the mean ± SD (n=8).(### p < 0.001,## p < 0.01,compared with the normal group;
***p < 0.001,**p < 0.01,*p < 0.05,compared with the model group).
2.1.2. Effects of RC on serum AST and ALT activities of mice with CCl4-induced liver
injury
The results are shown in the figure 1 and figure 2.
2.1.3. Effects of RC on serum SOD, GSH and MDA activities of mice with CCl4-induced
liver injury
The results are shown in the figure 3, figure 4 and figure 5.
2.3. Discussion
CCl4 has been widely used in animal models to investigate chemical toxin-induced liver dam-
age and as an excellent model to evaluate the efficacy of hepatoprotectants (Lin et al. 2014).
Serum hepatobiliary enzymes such as AST and ALT are present in high concentrations in the
NATURAL PRODUCT RESEARCH 2699
liver under normal conditions. When there is hepatocyte necrosis or membrane damage,
these enzymes will be released into the circulation, as indicated by elevated serum enzyme
levels (Vuda et al. 2012). When liver cells are attacked by free radicals, SOD and GSH-Px can
be reduced because of the excessive consumption, so that it can be understood that the
higher the activity of SOD and GSH-Px in the body, the faster the velocity of radical scavenging
is MDA, (Minor et al. 1993; Cigremis et al. 2009)the final product generated in the metabolism
of lipid peroxides when lipids are attacked by free radicals(Lu et al. 2012; Zhang et al.2013)
can further damage the cells, which can not only reflect the degree of sensitivity of lipid
peroxidation but also indirectly reflect the degree of cell injury (Pirinccioglu et al. 2010).
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4. Conclusions
From the biochemical indicators, it was showed ethyl acetate extracts were having the
strongest antioxidant activities and significant protective effect against acute hepatotoxicity
induced by CCl4, the order of hepatotoxicity effects of these extracts was ethyl acetate
extract > total extract > n-BuOH extract. According to the results of HPLC-EIS-MS/MS, Oleanic
acid, Phlorizin dihydrate and quercetin-3-rhamnoside may be considered as the main hepato-
protective factor in ethyl acetate extract.
Acknowledgements
The authors are thankful to Prof. Lu and Prof. Jia for their encouragement and support during the
course of study.
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