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Abstract:
A simple UV-Visible Spectrophotometric method was developed for the determination of chlorpheniramine maleate
in pure and its pharmaceutical formulations. chlorpheniramine maleate exhibited maximum absorption at 262nm in
0.1N HCl and obeyed linearity in the concentration range of 10-60 μg/ml. The proposed method was statistically
validated.All the proposed methods are simple, selective, reproducible, sensitive and accurate with good precision.
Some of the methods were proved to be superior to most of the reported methods. All these proposed methods for
estimation of selected drugs such as chlorpheniramine maleate were successfully applied either in bulk or
pharmaceutical formulations. The proposed methods can be used as alternative methods to the reported ones for the
routine determination of selected drugs under the study in bulk and pharmaceutical dosage forms.
Keywords: chlorpheniramine maleate, UV-Visible Spectrophotometer, Hydrochloric acid.
Please cite this article in press as Kirtimaya Mishra et al, New Analytical Method Development and Validation of
Chlorpheniramine Maleate by Using UV-Visible Spectrophotometry, Indo Am. J. Pharm. Sci, 2016; 3(7).
Parameter Chlorpheniraminemaleate
Sandell’s sensitivity
% Range of error
0.0021
95% confidence limits
0.0028
99% confidence limits
Estimation of Chlorpheneramine maleate in sonicated for 30 minutes. The volume was made up
commercial formulations to 10ml with HCl and filtered to get a solution of
For analysis of commercial formulations, 10 tablets concentration 1000µg/ml.
containing chlorpheniramine maleate were taken and This was further diluted with HCl to get a
powdered. The powder equivalent to 4mg of concentration within the linearity range and the
Chlorpheniramine maleate was taken in a 10ml absorbances were measured against the blank at
volumetric flask, containing 7ml of HCl and 262nm. The results were shown in Table 3.
Cadistin
1. (Zydus Chlorpheniraminemalate 4 4.902±0.0040 98.046
cadila)
Validation
Table 4: Precision data
routine determination of selected drugs under the of Salbutamol Sulphate and Ambroxol Hydrochloride
study in bulk and pharmaceutical dosage forms. By RP-HPLC and HPTLC In Bulk Drug and Dosage
Form. International journal of pharmacy and
ACKNOWLEDGEMENT: pharmaceutical sciences,2011;4 (4): 307-311.
The author was very thankful to the Elite chemicals 6.Akademiai Kiado.,.Simultaneous Analysis of
and SD Chemicals for supplying the pure drugs and Phenylephrine Hydrochloride, Guaiphenesin,
necessary chemicals for the proposed research work. Ambroxol Hydrochloride, and Salbutamol (As
Salbutamol Sulphate) By Use of A Validated High-
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