Thin Layer Chromatographi

PT. Abadi Nusa Usahasemesta

Jln. Surabaya No. 54. Jakarta
Jln. Raden Saleh No. 45 G. Jakarta

PT . DARMA KARYA MAKMUR SENTOSA

Jln. Jemur Andayani No. 50 Surabaya
 Plannar Chromatography

• Modern Thin Layer Chromatography

• Instrumental Thin Layer Chromatography
• High Performance Thin Layer Chromatography
• TLC / HPTLC
Why Planar Chromatogrphy today

• Clearly
• Rapid
• Reliable
• Flexible
• Cost efficient
• GLP Compliant
• Well documented
 Other Advantages

• Parallel analysis sample and standard

• Flexible and multi detection
• Offline technique adds flexibility
 Disadvantage

• Open system , Environmental effects

• Limited separation power.
• Limited gas phases control.
• Plate use only once
• Not fully Automated
 Possible Tasks

• Analysis or collection fraction

• Qualitative or Quantitative analysis
• All components must be separate
• Precission Method
• Simple and clear information
• Solving otherwise unsolving problem
 Key Point
• Solvent
• Stationary phase
• Compact starting zone
• Developing Solvent
• Gas Phase
• Developing techniques
• Detection
 Solvent

• Solubility
• Purity
• Boiling point
Stationary Phase

• Cellulose or Silica gell

• Code letters : G(Gypsum),S(Starch),O(Organic
binder),F(Fluorescentindicators),
RP(Reversed Phase),NH2(Amino) etc
• Particle size
• Support : Glass , Polyester, Aluminium
 Stationary Phase

• Silica gel 60 F (unmodified) : more than 80 %

are done with this layer
• Aluminium oxide : alkaloids and steroids
• Cellulose : amino acid , sugars ,antibiotics
• Silica gel (chemical modified)
 Stationary Phase
• Average Particle size
TLC : 10 – 15 um . HPTLC : 5 – 7 um
• Layer thicknes
TLC : 250 um . HPTLC : 100 – 200 um
• Separation distance
TLC : 150 mm . HPTLC : 70 mm
• Solvent consumption
• Detection limit
Compact Starting Zone

• Suitable solvent and reproducible

application
• Reduce speed application
• Focussing
• Apply bands instead spots
 Developing solvent
• Simple Composition
• Small polarity differents between component
• Non Toxic
• No Reactivity with sample
• Low Viscocity
• Define “ pure” quality
• Solvent strength
Developing techniques
Developing techniques
Developing techniques
Developing techniques
Developing techniques
Developing techniques
 Detection
• Densitometer
• Derivatization
• Others : - Spectrophotometer
- HPLC
- GC
- Mass Spectra
Spectrum Library

‘Analysis‘ spectrum
compared with a
spectrum from the library
 Thank you
Terima kasih