Live, Singlet Events (191+ 193+ 195- events)

A. Mock Infection γHV68 Infection IL10gfp Expression

CD4+ 1.25
Colored by phenotype:
20 CD3+ CD4+ MHCII-
25 CD3+ CD8+ MHCII-
CD11b+ Ly6C- Ly6G-
CD11b+ Ly6C+ Ly6G-
0 CD11b+ Ly6C+ Ly6G+
0 CD19+ MHCII+
CD45+
CD45-
-20
-25

tSNE2
tSNE2

0.0
-25 0 25 -20 0 20
tSNE1
tSNE1

CD4TTcells
CD4 cells(191+
(191+193+
193+195-
195-CD3+
CD3+CD4+
CD4+MHCII-
MHCII-events)
events)

γHV68 Infection
B. 4,142 events
Mock Infection γHV68 Infection

10
20 Mock Infection
0 1,322 events

-20
-20

% ts
tSNE2

of even
-20 0 20 -20 0 20
tSNE1
%o
f e v e nts

C. CD44 Ki67 D.
Enriched in Enriched in
Mock Mock
mock infection γHV68 infection
Cluster ID

γHV68 γHV68
#12 #13 #14 #15 #1 #2 #4 #5
0 7 0 6

PD-1
Hi Lo Hi Lo Hi Mid Hi Hi CD44
ICOS
Mock Mock
Hi Lo Lo Lo Hi Lo Hi Hi Ki67

Lo Lo Lo Lo Mid Lo Mid Hi ICOS

Protein Markers

γHV68 γHV68

0 5.5 0 4
Lo Lo Lo Lo Hi Lo Mid Hi PD-1
Lag3 Ly6C
Mock Mock
Lo Lo Lo Lo Hi Lo Lo Hi Lag3

Lo Mid Lo Lo Hi Mid Mid Lo Ly6C

γHV68 γHV68

0 6 0 6
Lo Lo Mid Lo Mid Lo Mid Mid CD49b
CD49b IL10gfp

- - - - +/- - - +/-
Mock Mock
IL10gfp

9.4% 34.2% 6.3% 32.7% 0.1% 0.6% 0.3% 0.0% % of mock

γHV68 γHV68
tSNE2

0 2.5 0 3 0.1% 0.1% 0.1% 0.1% 19.7% 18.8% 24.5% 19.2% % of infected
tSNE1
Kimball et al
Figure 2