CD4 T cells (191+ 193+ 195- CD3+ CD4+ MHCII- events), stimulated for ICCS

A. B.
B6 IL10KO
CD44 IFNγ TNFα IL-10
20

-20
tSNE2

-20 0 20 -20 0 20 0 5.5 0 5 0 6 0 2.5

tSNE1

C. D. **
B6 IL10KO
60 B6
IL10KO ***
20

% of events
Colored by cytokine profile:
IFNγ-, IL-10-, TNFα-
IFNγ-, IL-10-, TNFα+ 40
0
IFNγ+, IL-10-, TNFα-
IFNγ+, IL-10-, TNFα+ ns
-20
IFNγ-/+, IL-10+, TNFα-/+ 20
tSNE2

ns
-20 0 20 -20 0 20 ****
tSNE1
0

-/+
Fa

Fa
Fa

Fa

Fa
TN

N
TN

TN
-T

N
,T
-,

-,

-,
10
10

10

10

0+
IL
IL

IL

IL

L1
+
-,

-,

+,

,I
N
N

-/+
N

IF

N
IF

IF

IF
E.

N
IF
CTLA-4 GITR ICOS Lag3 PD-1
IFNγ+
TNFα+
B6

0 5 0 4 0 4 0 2.5 0 3
IL10KO

0 5 0 3.5* 0 4 0 3* 0 3

Kimball et al
Figure 5