Cluster 1

Unit of Competency  Qualify laboratory equipment, apparatus and glass wares

covered  Operate laboratory equipment and apparatus
 Maintain equipment laboratory equipment and apparatus
Set B
Written Examination

1. d 11. d

2. a 12. c

3. b 13. a

4. a 14. b

5. b 15. b

6. a

7. c

8. a

9. b

10. b

Matching Type

16. d 21. e

17. c 22. d

18. b 23. c

19. a 24. b

20. e 25. a
Problem Solving

26. y = 0.1061 x + 0.0004

27. r2 = 0.999

28. 42.6 ppm

29. 85.2 %

30. 14.8 %

Multiple Choice

1. Which of the following is not an ideal place to install the analytical balance?
a. Stable and away from vibrations.
b. Away from drafts caused by air conditioner.
c. Away from direct sunlight.
d. None of the above.

2. It is a general method for determining the concentration of a substance in an unknown

sample by comparing the unknown to a set of standard samples of known concentration.
a. Calibration curve
b. Method validation
c. Titration
d. Chromatography

3. Which of the following instruments uses a cuvette?

a. Flame- AAS
b. UV-Vis Spectrophotometer
c. Gas Chromotograph
d. pH Meter.

4. A series of reference standards solutions that have known and accurate pH values at
different temperatures used for pH meter calibration.
a. Buffer Solutions
b. QC Solutions
c. pH solutions
d. None of the above.
5. A property of a wave which is described as the distance between a point on one wave and
the same point on the next wave.
a. frequency
b. wavelength
c. amplitude
d. period

6. Which of the following should not be done in using a cuvette?

a. Use a brush to clean the insides of the cuvette

b. Fill the cuvette about 3/4 full of the solution you wish to test.
c. Wipe the outside of the cuvette with a lint-free, soft tissue to remove any moisture or
fingerprints from the outside surface.
d. Do not clean your cuvettes in an ultrasonic cleaning bath.

7. It is the measure of hydrogen ion activity in aqueous solutions.

a. Conductivity
b. Viscosity
c. pH
d. Solubility

8. Law that states the linear relationship between absorbance and concentration of an
absorbing species
a. Beer-Lambert’s Law
b. Charles Law
c. Boyles Law
d. Avogadro’s Law

9. Type of quality-control sample used to evaluate the effects of sample matrices on the
performance of an analytical method
a. Matrix Duplicate
b. Matrix Spike
c. Method Blank
d. Reagent Blank
10. A clean sample (e.g., distilled water) processed through the instrumental steps of the
measurement process to determine instrument contamination.

a. Method Blank
b. Instrument Blank
c. Surrogate
d. Reagent Blank

11. Minimum concentration of a substance that can be measured and reported with 99%
confidence that the analyte concentration is greater than zero

a. Limit of Quantitation
b. Lower Control Limit
c. Lower Warning Limit
d. Method Detection Limit

12. Component of error which, in the course of a number of analyses of the same measurand,
remains constant or varies in a predictable way.
a. Random Error

b. Analyst’s Error
c. Systematic Error
d. Uncertainty

13. Component of error which arises from unpredictable variations of influence quantities.
a. Random Error

b. Analyst’s Error
c. Systematic Error
d. Uncertainty
14. Series of analytical measurements used to assess the quality of the analytical data

a. Quality Assurance
b. Quality Control
c. Analytical run
d. Fortification

15. Determine the actual volume contained in a 50.0 mL volumetric flask given the following
data:

Mass of water: 50.1227 g

Density of water at 25˚ C : 0.99707 g/mL

a. 50.45 mL
b. 50.27 mL
c. 50.37 mL
d. 50.17 mL
Matching Type

A. Match the following parts of the Atomic Absorption Spectrophotometer with their basic
functions.

16. Light D a. Separates and transmits a narrow portion of the

optical signal chosen from a wider range of wavelengths
available at the input.

17. Nebulizer C b. Destroys any analyte ions and breakdown complexes

to create atoms (the elemental form) of the element of
interest.

18. Flame c. Creates a fine aerosol for introduction into the flame.
B

19. Monochromator A d. Provides the analytical light line for the element of
interest

20. Detector E e. Determines the intensity of photons of the analytical

line exiting the monochromator.

B. Match the following glassware with their corresponding uses.

21. Desiccator E a. measuring volume of liquids

22. Volumetric flask D b. mixing, stirring, and heating liquids
23. Pipette C c. measuring and transferring volume of liquids from
one container to another
24. Beaker B d. precise dilutions and preparation of standard
solutions
25. Graduated Cylinder A e. protect chemicals which are hygroscopic or which
react with water from humidity

Problem Solving

The linearity of the copper analysis using Flame-AAS was determined by running standard
concentrations. The table below shows the data generated.

Copper Concentration, µg/mL Absorbance

0.00 0.00
2.000 0.215
4.000 0.431
6.000 0.618
8.000 0.859

26. Calculate the equation of the line.

27. Calculate for the linearity of the calibration curve.

28. Calculate for the copper concentration of the Quality Control Sample if its average
absorbance is 0.452 after diluting it ten folds.

29. Calculate for the percent recovery obtained for the QC Sample run if the theoretical value
is 50.0 ppm.

30. Calculate for the percent error obtained for the QC sample run if the theoretical value is
50.0 ppm.