Академический Документы
Профессиональный Документы
Культура Документы
Showing Recovery of Added Uric Acid and Also Agreement between Results
Obtained by Direct Method, Indirect Method, and Micromethod on
Filtrates from Unlaked Human Blood
The figures ind,icate the uric acid found before and after addin ‘!z 5 mg.
-
A+5 B B+5 C c+5 D D+5
_-
1 3.5 8.3 3.44 8.2 3.5 8.2 3.49 8.44
2 3.2 8.15 3.1 7.85 3.2 8.1 3.1 8.0
3 3.7 8.4 3.55 8.15 3.6 8.3 3.6 8.4
4 2.05 6.95 2.0 7.0 2.1 6.8 2.1 7.0
5* 3.2 8.2 3.2 7.65 3.2 7.65 3.3 8.0
6 3.5 8.36 3.5 8.2 3.5 8.1 3.5 8.4
7* 2.9 7.65 2.9 7.44 2.9 7.65 3.0 7.85
8 2.9 7.8 2.9 7.75 2.9 7.75 3.1 8.05
9 2.05 7.0 2.1 6.9 2.0 7.04 2.05 7.0
10 2.3 7.15 2.3 7.0 213 7.15 2.35 7.2
-
A =_ direct macromethod; B = determination on the silver precipitate;
C = determination on the extract from the silver precipitate; D = micro-
method.
* Not repeated with less than 5 cc. of filtrate.
traction method calls for two extra centrifugings and requires neat
work, but if carefully done gives all the uric acid.
All through the rather long period covered by this research, Dr.
C. L. Derick, of the Peter Bent Brigham Hospital, has supplied us
with all the hospital bloods we could use. In view of the very
great demand for space in this Journal the original plan of giving a
comprehensive series of analyses has been abandoned. In Table
I we give therefore in highly condensed form, only a few analyses
of bloods in which the uric acid was determined before and after
the addition of uric acid, by each of the four methods described
0. Folin 119
above. The significance of these figures is so clear that comments
would seem to be superfluous. It need be stated, however, that
the first macrodeterminations made on the filtrates containing
added uric acid would sometimes be too low by 0.2 to 0.3 mg. per
cent, but these deficits disappeared when the determinations were
repeated with less than 5 cc. of the blood filtrates. In other
words, when 8 mg. or more of uric acid are found by the regular
method involving the use of 5 cc. of blood filtrate and the 0.02 mg.
standard, the determination should be repeated with less than 5
And these errors are likely to be larger still, if the uric acid reagent
is not completely free from phenol reagent.
Indirect Method-Two solutions, in addition to those used in
blood analysis, are required; both keep indefinitely.
1. A 5 per cent solution of silver nitrate. This solution, even
if perfectly clear when first prepared, may develop a slight color on
standing. This color is most quickly produced by heating to 100”
for 2 hours in a flask covered with a beaker. After cooling, add a
few cc. of a solution containing 50 mg. of sodium chloride, shake
Alerts:
• When this article is cited
• When a correction for this article is posted