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CURVA DE CALIBRADO Y=1.

5759X+3*10^(-16)
BIOMASA
N° t(horas) ABS (640nm) Dilución X (g/L) LN(X)
1 0 0.024 1 0.015229 -4.184528
2 2 0.064 1 0.040612 -3.2036987
3 4 0.051 1 0.032362 -3.4307562
4 6 0.061 1 0.038708 -3.251708
5 8 0.145 1 0.092011 -2.3858481
6 9 0.263 1 0.166889 -1.7904278
7 11 0.641 1 0.406752 -0.8995524
8 14 0.797 4 2.022971 0.70456722
9 24 0.746 10 4.733803 1.55472888
10 27 0.956 15 9.099562 2.2082263
11 30 0.62 20 7.868520 2.06286993

Curva de Biomasa
10.000000
9.000000
8.000000
7.000000
6.000000
X (g/L)

5.000000
4.000000
3.000000
2.000000
1.000000
0.000000
0 2 4 6 8 10 12
Tiempo (horas)

PROTEÍNAS

Curva de Calibrado Calibracion de Proteí


0.18
N [] (g/L) ABS 0.16 f(x) = 0.3391549296x - 0.000211267
1 0.5 0.17 0.14 R² = 0.9977419821
2 0.4 0.137 0.12
0.1
ABS (nm)

3 0.3 0.1
0.08
4 0.25 0.08
0.06
5 0.2 0.071
0.04
6 0.1 0.033 0.02
7 0.05 0.018 0
8 0 0 0 0.1 0.2 0.3
[] (g/L)
0
0 0.1 0.2 0.3
[] (g/L)

Datos obtenidos en la experiencia

N ABS (595nm) P (g/L) Obtención de P


1 0.011 0.033019
2 0.005 0.015330 0.180000
0.160000
3 0.025 0.074292
0.140000
4 0.031 0.091981 0.120000
5 0.030 0.089033

P (g/L)
0.100000
6 0.035 0.103774 0.080000
7 0.037 0.109670 0.060000
8 0.037 0.109670 0.040000
9 0.035 0.103774 0.020000
0 5 10 15
10 0.058 0.171580
11 0.036 0.106722 Tiempo (h

pH
N° t(horas) pH
Seguimiento de pH
1 0 4.04
2 2 4.01 4.2
3 4 4 4
4 6 4 3.8
5 8 4 3.6
3.4
pH

6 9 4
7 11 3.97 3.2
8 14 3.57 3
9 24 2.92 2.8
0 2 4 6
10 27 2.87
11 30 2.84 Tiempo (horas)

Seg
% OD 120
N° t(horas) %OD
1 0 101.5 100
2 2 100.7
80
3 4 99.9
4 6 96.7 60
pH

5 8 88.8
6 9 80 40
7 11 41
8 14 0 20

9 24 0
0
10 27 0 0 2 4
20

0
0 2 4
11 30 0
LN(X/Xo)
0
Linealidad de la Concentración de Biomasa
0.98082925 7
0.7537718 6 f(x) = 0.7021817362x - 1.1754830435
0.93282003 5 R² = 0.9448812457
1.79867991 4

LN(X/Xo)
2.3941002 3
3.28497563 2
4.88909521 1
5.73925686 0
6.39275428 0 2 4 6 8 10 12
6.24739792 Tiempo(horas)

μM 0.7022
td 0.98710792
t 8.89689251

10 12

libracion de Proteínas

= 0.3391549296x - 0.0002112676
0.9977419821

.1 0.2 0.3 0.4 0.5 0.6


[] (g/L)
.1 0.2 0.3 0.4 0.5 0.6
[] (g/L)

Obtención de Proteínas
Qx :
00
00
Xf 7.868520 Tf
00
00 Xi 0.015229 Ti
00
00 Qp :
00
00 Pf 0.106722 Tf
00 Pi 0.033019 Ti
0 5 10 15 20 25 30 35
Tiempo (horas)

Seguimiento de pH

4 6 8 10 12
Tiempo (horas)

Seguimiento %OD
120

100

80

60

40

20

0
0 2 4 6 8 10 12
Tiempo (horas)
20

0
0 2 4 6 8 10 12
Tiempo (horas)
30 Qx ∆X/∆t 0.26177634
0

30 Qp ∆P/∆t 0.00245676
0
VELOCIDAD DE AGITACIÓN
C 300 400 500
0 45.4 79.6 77.9
5 45.5 79.5 77.9
10 45.3 79.6 77.9
15 45.3 79.5 78
20 45.1 79.4 77.9
25 44.6 79.2 77.6
30 44 78.9 77.3
35 42.4 78 76.4
40 42.1 77.6 75.5
45 40.4 76.6 74.4
50 39.2 76.2 73.4
55 37.7 75.2 72.2
60 36.6 74.3 70.5
65 34.9 73.3 69.3
70 33.6 72.2 67.8
75 31.9 71.2 66.5
80 30.3 70.2 65
85 28.4 69.1 63.4
90 27.3 68.2 62
95 25.7 67.2 60
100 24.2 66.4 58.9
105 22.3 65.2 57.2
110 21 64.1 55.8
115 19.2 63 54.1
120 17.5 62.2 52.5
125 16.5 61.1 50.9
130 14.6 60.1 49.2
135 12.9 58.9 47.5
140 11.9 58.2 45.7
145 11.2 56.4 44.2
150 10.8 55.9 42.4
155 11 55 40.9
160 11.3 53.9 39
165 12.1 52.7 37.5
170 12.6 51.9 35.8
175 12.3 51 34.2
180 13.9 50 32.6
185 14.7 49.8 30.8
190 15.5 47.7 29.2
195 16.4 46.8 27.3
200 17.3 46 25.5
205 18 45.1 24
210 19 44 22.1
215 19.5 43 20.6
220 20.6 42 19.7
225 20.5 41.1 16.2
230 22.1 39.9 15.1
235 22.9 39.8 12.9
240 23.4 38 13.4
245 24.1 37 14.3
250 24.7 36 16.2
255 25.5 34.8 19
260 25.9 34 22.8
265 26.4 34.2 27.2
270 26.9 32.3 30.7
275 27.7 31.5 35.3
280 28.3 30.6 38.6
285 28.4 29.4 41.8
290 29 28.2 45.2
295 29.7 27.2 48.6
300 30 26.5 51.2
305 30.8 25.3 53.8
310 31.1 24.6 56.3
315 31.4 23.7 58.2
320 31.8 22.6 60.2
325 32.1 21.7 61.9
330 32.5 20.8 63.3
335 32.8 19.3 64.9
340 33 18.5 66.1
345 33.4 18.2 67.2
350 33.6 18.9 68.2
355 33.8 19.9 69.2
360 34.2 21.5 70
365 34.5 23.4 70.7
370 34.7 26 71.3
375 34.7 28.2 71.9
380 35.1 30.9 72.5
385 35.2 32.8 73
390 35.6 35.2 73.5
395 35.8 36.9 73.9
400 36 39 74.2
405 36.1 41.1 74.6
410 36.3 43.5 74.8
415 36.4 45.1 75.1
420 36.6 46.9 75.2
425 36.7 48.6 75.4
430 36.9 50 75.4
435 37 51.4 75.7
440 37.2 52.8 75.8
445 37.2 54 75.9
450 37.3 55.8 76
455 37.4 56.7 76.1
460 37.5 57.9 76.2
465 37.8 58.8 76.2
470 37.8 60 76.3
475 37.9 61.9 76.3
480 38.1 62.7 76.3
485 38.2 63.3
490 38.4 64
495 38.5 64.8
500 38.5 65.5
505 38.5 66.1
510 38.6 66.8
515 38.7 67.2
520 38.7 67.7
525 38.9 68.4
530 38.9 68.8
535 39 69.2
540 39 69.6
545 39 70
550 38.9 70.8
555 39 71.1
560 38.9 71.3
565 39.1 71.6
570 39.1 71.9
575 39.1 72.7
580 39 73
585 39.1 73.2
590 39.2 73.5
595 39.2 73.7
600 39.1 74
605 39.2 74.1
610 39.3 74
615 39.2 74.1
620 39.3 74.4
625 39.3 74.3
630 39.3 74.5
635 39.3 74.7
640 39.3 74.6
645 39.3 74.7
650 39.3 74.8
655 39.3 74.9
660 39.2 75
665 75
670 75.2
675 75.3
680 75.3
685 75.3
690 75.4
695 75.4
700 75.5
705 75.4
710 75.4
715 75.4
720 75.5
725 75.6
730 75.6
735 75.6
740 75.6
745 75.7
750 75.6
755 75.7
760 75.7
765 75.6
770 75.7
775 75.8
780 75.9
785 75.8
790 75.9
795 75.8
800 75.7
805 75.7 OD %
90
80
70
60
50
OD%

40
30
20
10
0
0 100 200 300

300 rpm
0
0 100 200 300

300 rpm
OD % vs TIEMPO

100 200 300 400 500 600 700 800 900

TIEMPO
300 rpm 400 rpm 500 rpm
100 200 300 400 500 600 700 800 900

TIEMPO
300 rpm 400 rpm 500 rpm

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