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The information in this operator’s guide was correct at the time of printing.
However, Siemens Medical Solutions Diagnostics continues to improve
products and reserves the right to change specifications, equipment, and
maintenance procedures at any time without notice.
If the system is used in a manner differently than specified by
Siemens Medical Solutions Diagnostics, the protection provided by the
equipment may be impaired. See warning and hazard statements.
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ADVIA Centaur XP Operator’s Guide: Contents
Contents
1 System Overview
3 Calibration
4 Quality Control
5 Performing Maintenance
7 Managing Data
Appendix D: Supplies
Appendix E: Specifications
Appendix H: Glossary
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ADVIA Centaur XP Operator’s Guide: Contents
Index
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Using This Guide
Organization
The following table describes how this operator’s guide is organized.
If you want to . . . Then refer to . . .
learn about system features such as no-pause reloading Section 1:
and onboard refrigeration, System Features
learn about user interface components, how to use Hardware Overview,
online information, and view software maps, Software Overview,
review the hardware and the operating sequence, Technology.
review principles of chemiluminescence and assay
reaction formats,
process samples, monitor status, or manage sample Section 2:
results, Operating the System.
review Master Curve and 2-point calibration principles, Section 3:
define Master Curves and calibrators, Calibration.
process calibrators,
learn about accessing ADVIA QC, Section 4:
define quality control materials, Quality Control.
perform scheduled maintenance activities, Section 5:
record maintenance activities, Maintenance.
investigate and correct system problems, Section 6: Troubleshooting.
learn about saving results data files to an archive, Section 7:
learn about backing up your system configuration files, Data Management.
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Conventions
The ADVIA Centaur XP Operator’s Guide uses the following text and symbol
conventions:
Convention Description
BIOHAZARD Biohazard statements alert you to potentially
biohazardous conditions.
LASER WARNING: Laser Warning statements alert you to the risk of
exposure to lasers.
WARNING: Warning statements alert you to conditions that
may cause personal injury.
CAUTION: Caution statements alert you to conditions that may
cause product damage or loss of data. On the
system, this symbol indicates that you should refer
to the operator’s guide for more information.
NOTE: Note statements alert you to important information
that requires your attention.
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ADVIA Centaur XP Operator’s Guide: Using This Guide 3
Convention Description
Bold Bold type indicates commands on the user
interface, keys, or the exact text that an operator
needs to type.
For example, if the word save appears as Save, it
refers to the selecting the Save button on the user
interface.
Another example is typing a specific entry into a
text box. If the word welcome appears as welcome,
it means that you should type that word into the
specified field.
Italic Italic type refers to the title of a document or a
section title in this operator’s guide. For example,
Operating the ADVIA Centaur XP System refers to
Section 2 of this operator’s guide.
Terminology
The following table explains some of the special terminology used in this
operator’s guide and the specific actions that you need to take when you see the
terminology:
Term Description
Select To select an item, use your finger to touch the item on the touch-screen
monitor or select the item with the system pointing device. The
background of the item changes color or displays a black frame to
indicate that you selected the item.
Enter Type the specified information using the keyboard and then press
the Enter key.
Scan Move the hand-held barcode scanner over the specified barcode to enter
the information.
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ADVIA Centaur XP Operator’s Guide: System Overview
1 System Overview
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System Features
Intended Use
This system is intended for professional use in a laboratory environment only.
Tests performed using this system are intended for in vitro diagnostic use. As with
all diagnostic tests, a definitive clinical diagnosis should not be based on the
results of a single test, but should only be made by the physician after all clinical
and laboratory findings have been evaluated.
The ADVIA Centaur XP has many features for enhancing laboratory operations:
Feature Description
No-pause Reloading You can load samples, reagents, and supplies at any time,
even while the system processes samples.
Optimal Productivity The throughput is up to 240 tests per hour and the walkaway
time is up to 840 tests.
Automatic Reflex The system automatically schedules reflex tests based on
Testing parameters that you define.
Onboard Refrigeration Reagents remain in the refrigerated reagent areas, so you can
process samples without time-consuming system
preparation.
True Stat Capabilities The system efficiently processes Stat requests via a
dedicated Stat port without disrupting sample processing.
Clot Detection and The system ensures sample integrity by detecting complete
Management obstructions in the sample probe, notifying you of the
condition, and then performing management and recovery
tasks.
Bubble Detection The system ensures the integrity of the reagent aspiration
and dispense in the reagent and the ancillary probes.
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Feature Description
Automated Cleaning The system automatically performs the daily cleaning.
Autoschedule Daily You can set up the system to automatically schedule and log
Cleaning the daily cleaning procedure in the Maintenance Log.
User Interface The intuitive graphical user interface is via a touch-screen
monitor making it possible for you to perform your daily
tasks directly on the screen without using the keyboard.
Context-sensitive help is available for any window or field.
LIS Interface Bidirectional laboratory information system (LIS) interface
allows you to send to and receive information from your
LIS. The system supports the ASTM interface protocol.
Laboratory Automation The ADVIA Centaur XP STS sample transport system is an
optional feature that enables you to use the
ADVIA Centaur XP system with any laboratory automation
system.
RealTime Solutions The ADVIA Centaur XP system has integrated, pro-active
service service features that assist in problem detection and
remotely initiated diagnosis of problems, having the effect
of faster support resolution. The system supports remotely
initiated activities like software updates and on-line
documentation updates.
Visible Status Light The ADVIA Centaur XP system has a status light that is
visible from most positions in the room. The status lights
correspond to events that occur on the ADVIA Centaur XP
system.
Hardware Overview
The Hardware Overview section introduces the operating sequence of the system
and describes the location and function of the major subsystems and components.
This information helps you to perform the following tasks:
• operate the ADVIA Centaur XP system for maximum efficiency
• perform the maintenance tasks
• understand the basic test processing sequence, which is essential for effective
troubleshooting
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The ADVIA Centaur XP system uses several areas of the system while processing
samples, as shown in Figure 1-2.
Off No error or warning conditions exist and the ADVIA Centaur XP user
interface screen saver and status buttons are neutral.
• The system is turned off.
• The light is disabled.
NOTE: If the status light is off for an extended period of time, check the
user interface error conditions.
Yellow At least 1 warning condition exists.
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Red At least 1 critical condition exists that may cause the system to stop
aspirating or processing samples.
Blinking • A new event has occurred.
Yellow or • When you open the status window to view the new condition, the
Blinking status light maintains the current color and stops blinking.
Red • When you correct all warning or critical conditions, the status light
turns off.
• When you correct all critical conditions and warning conditions
remain, the status light changes to steady yellow.
Blinking A remote update is in progress.
Green If an error occurs during a remote update, the status light changes to
blinking red.
Refer to Enabling the Visible Status Light‚ page 8-44.
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Samples are placed in the sample racks, which hold 5 primary sample tubes
or sample cups.
1 Tube-type selector
2 Sample rack
You load the racks in the sample entry queue and remove them from the sample
exit queue. You can enter stat samples into the stat queue.
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Racks enter the inprocess queue where the sample barcode scanner reads the
barcode labels on the rack and on each sample cup or tube. When the sample rack
is in the inprocess queue, the sample probe aspirates the sample.
After each sample in the rack is aspirated, the rack moves from the inprocess
queue to the sample exit queue.
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Light-emitting diodes (LEDs) on the primary reagent door indicate the status of
the primary packs:
• Green indicates that the reagent pack is loaded and available for use.
• Blinking green indicates that the system detected that a reagent pack is
present but cannot read the barcode.
• Neutral indicates that no reagent pack is loaded in that position or that the
pack is depleted, expired, inactivated, or has no barcode.
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You load ancillary reagent packs in the ancillary reagent entry. The system
delivers the packs to the ancillary reagent queue. The ancillary reagent queue
stores the packs at a temperature of 4° to 8°C (39° to 46°F). You remove ancillary
packs at the ancillary reagent entry.
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The sample probe aspirates sample from a container in the inprocess queue and
then dispenses sample into a cuvette in the incubation ring.
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The incubation ring is a circular, insulated track that advances the cuvette
at 15-second intervals and incubates the cuvette at 37°C (98.6°F).
1 Magnets
2 Incubation ring
The incubation ring moves the cuvette from the sample probe to the ancillary
probe and to the reagent probes.
The ancillary probe aspirates the ancillary reagents from the packs in the ancillary
reagent queue and dispenses the reagents into the cuvette in the incubation ring.
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The rinse station rinses the probe with reagent water before each ancillary reagent
aspiration.
1 Ancillary probe
2 Rinse station
The incubation ring moves the cuvette to the reagent probes. The 3 reagent probes
move independently of each other to aspirate primary reagents from the primary
packs and to dispense the reagents into the cuvette in the incubation ring.
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Between samples, the rinse stations rinse the probes with reagent water.
1 Reagent probe
2 Rinse station
The incubation ring moves the cuvette to the wash station. After the magnetic
particles are pulled to the side of the cuvette by magnets positioned along the
incubation ring, the aspirate probes draw fluid out of the cuvette. The wash
station dispenses wash fluid into the cuvette to wash the magnetic particles.
1 Aspirate Probe 1
2 Aspirate Probe Clip
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The incubation ring moves the cuvette to the acid probe, which dispenses acid
reagent into the cuvette. The incubation ring moves the cuvette to the elevator,
which lifts the cuvette from the incubation ring into the luminometer.
The luminometer has 3 parts: a photomultiplier tube (PMT) with photon-counting
electronics, a base probe, and a waste probe. When base reagent is dispensed into
the cuvette, the chemiluminescent reaction occurs. The luminometer measures the
light emitted as relative light units (RLUs). The central processing unit (CPU)
processes the data from the PMT and converts the RLUs into results. The system
sends the results to an LIS or a printer.
The waste probe aspirates the waste fluid from the cuvette and the cuvette drops
into the cuvette waste bin. As cuvettes exit the incubation ring, the system
continuously adds new cuvettes to the incubation ring.
You load cuvettes in the cuvette loading bin, then the system moves the cuvettes
to the orientation chute. The orientation chute correctly positions the cuvettes
before they enter the vertical cuvette chute. The vertical cuvette chute connects to
the preheater, which warms the cuvettes to a temperature of 37°C (98.6°F) before
the cuvettes enter the incubation ring.
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Computer Hardware
The ADVIA Centaur XP computers control system functions and processes data.
The applications module is equipped with a CD-RW drive for software updates
and for data storage. The computer has several serial ports, as well as an Ethernet
port that expands the communication link between Siemens and your laboratory,
allowing for enhanced remotely initiated diagnostics and proactive service. The
monitor, keyboard, mouse, and barcode scanner are all connected to the computer.
Keyboard
The keyboard offers special icon keys.
You can use the keyboard to make selections, open windows, and enter
information.
If you want to . . . Then . . .
open a window from the Press the symbol key for the status or the task area.
workspace, Press the key of the underlined character of the window
title.
close a window, press the Close Win key.
close all windows, press the Close All key.
go to the previous press the Prev Win key.
window,
go to the next window, press the Shift key and the Prev Win key.
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Barcode Scanner
LASER WARNING
Never look directly at the beam of a hand-held barcode scanner or point the
scanner at another person. Also, do not look at the reflection of the beam from a
shiny surface. Only trained field service personnel should perform procedures
related to laser assemblies. For more information about laser safety, refer to
Appendix A, Protecting Yourself from Barcode Scanner Lasers.
Use the hand-held barcode scanner to enter barcoded information, such as the
information on Master Curve cards or Calibrator Assigned Value cards. Place the
scanner on the top barcode of the Master Curve card or Calibrator Assigned Value
card. Slowly move the scanner down the card over the barcodes. The scanner
beeps after successfully reading a barcode.
You can also use the hand-held barcode scanner to enter the Sample ID for patient
samples. Refer to Scheduling Patient Samples Using a Hand-Held Barcode
Reader‚ page 2-52.
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Monitor
NOTE: The touch screen may not respond correctly if it is wet or if your finger or
gloves are wet.
The graphical user interface is via a touch-screen monitor, making it possible for
you to perform your daily tasks directly on the screen without using the keyboard.
The monitor is located on a flexible arm so you can adjust the position of the
monitor. The area of software called the workspace is always visible on the
monitor.
Software Overview
Use the ADVIA Centaur XP user interface workstation to control the operation of
the system. The user interface workstation consists of hardware and software that
you use to interact with the system.
Workspace
The workspace provides access to all system functions and information that an
operator requires to operate the system. The workspace is similar to a benchtop.
At a benchtop, you can pull several reports (windows) from different files
(workspace buttons), and spread them out to review or to enter information.
Although you can work on only 1 document at a time (active window), the others
are ready for you. You can return the report to the file (close the window) or you
can leave the report on the benchtop (leave the window open).
The workspace has 2 views:
• system view, offering access to system functions
Unless otherwise specified, when the procedure says “At the workspace,...” it
refers to the system view workspace.
• applications view, offering access to a set of applications including the
ADVIA QC analyzer, the RealTime Solutions service, and the online
documentation browser
To change from the 1 view to the other view, press the QC symbol key on the
system keyboard.
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Workspace Toolbar
The toolbar buttons are grouped into 3 areas at the top of the workspace: status
buttons, task buttons, and function buttons. These buttons open windows that
contain related information and tasks.
1 Status buttons
2 Task buttons
3 Function buttons
Status Buttons
The status buttons open windows that provide information on the condition of the
entire system, specific system areas, supplies, and reagents. The status buttons are
only available in the system view.
• The System Status button provides access to diagnostic tools and the system
information
• The Supplies Status button opens a window that displays information about
the cuvette supply, the sample tip supply, system fluid levels, and waste
levels.
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• The Sample Status button opens a window that displays information about the
status of samples that are in process.
• The Exception Status button directs you to the applications view and the
ADVIA QC application.
• The Reagent Status button opens windows that display information about the
onboard primary and ancillary reagents.
• The Maintenance Status button opens a window that accesses the maintenance
schedule, the maintenance log, the maintenance definitions, and the
maintenance procedures.
• The Event Log Status button opens a window that displays the events that
occurred on the system and provides access to troubleshooting procedures.
Refer to Monitoring Status in Section 2.
Task Buttons
The task buttons provide access to windows associated with specific tasks. When
selected, each button displays a list of the windows that are available.
1 Worklist button.
2 Quality Control button
3 Calibration button
4 Setup button.
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Function Buttons
The function buttons provide additional capabilities related to the workspace,
such as signing in, closing all windows, and requesting printed reports.
1 Close All
2 Sign In
3 RealTime Solutions - available in the Applications View
4 Instructions for Use - available in the Applications View
5 Print
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Software Windows
The windows are designed with a common organization. because the purpose of
each window is unique, the organization of each window may vary to best achieve
the objective of each window.
• The title bar indicates the task area and the name of the specific window. The
title bar also contains buttons that access window help, move the window
behind other windows, or close the window.
• The View, Sort, and Search fields control the amount or type of information
that is displayed, control the order of information displayed, and locate
specific items.
• The information display area displays the information that you selected in
View, Sort, and Search fields.
• The buttons on the bottom of the window perform actions on all information
in the information display area or in open related windows.
• The buttons on the side of the window perform actions or open windows that
provide more information about an item you selected in the information
display area.
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Moving Windows
When you open multiple windows, you can move the windows to more easily
review information or perform tasks
To move a window to a new position in the workspace:
• Using the touch screen, hold your finger on the window title bar and drag the
window to a new position.
• Using the system mouse, position the pointer on the title bar and hold down
the left mouse button while dragging the window to a new position.
Window Elements
Window elements enable you to make selections and interact with the windows.
To move a window behind other open windows, select the Move Back button.
• Tables allow you to review information and to select items.
• Dropdown lists display options.
• Data fields allow you to enter or display information.
• Scroll bar arrows scroll the view to display items that are not visible in the list.
• You can usually select as many options as required in a table.
A check mark displays next to the selected options.
• You can enable 1 item from the group of options using a diamond option
button.
• You can turn an option on or off by selecting or deselecting the option.
Online Information
The ADVIA Centaur XP system provides online help and procedures to answer
your questions about operating the system, maintaining the system, and correcting
system problems.
The system also provides online documentation that you can view and print as
necessary.
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Help
To access help for a window element, place the pointer on field or button and
press the right mouse button. You can also move the cursor to a window element
and press the ? key on the keyboard.
Window Help
Window help provides information about options available at each window and
brief instructions for using the window. To access window help, touch the ?
button in the title bar or select the ? using the mouse.
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Maintenance Procedures
To access maintenance procedures, select a maintenance activity at the
Status – Maintenance window, and then select Procedure. Refer to Performing
Maintenance‚ page 5-1.
Software Maps
Workspace buttons open windows that contain related information and tasks.
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System
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Status
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Worklist
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Calibration
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Technology
Chemiluminescence is a chemical reaction that emits energy in the form of
light.1–5 When used in combination with immunoassay technology, the light
produced by the reaction indicates the amount of analyte in a sample.
Direct chemiluminescent reactions directly measure the light energy without the
use of added steps or amplifying molecules.6 The ADVIA Centaur XP assays use
acridinium ester (AE) as the chemiluminescent label, because AE does not require
the addition of a catalyst or substrate.6
Direct Chemiluminescence
It is easy to automate direct chemiluminescence using AE and provides many
benefits, such as long reagent shelf life, fast reaction time, and assay sensitivity.
The ADVIA Centaur XP assays use the dimethyl form of AE because its stability
allows long reagent shelf life.6,8
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1 Light intensity
2 Seconds
3 Acridinium ester
4 Other methods
The AE molecule is much smaller than the alkaline phosphatase molecule used
in EIA assays. This smaller size decreases blockage of binding sites, increases
diffusion rates, and increases the assay sensitivity.8
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1 Antigen
2 Binding site
3 Antibody
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During incubation, coated PMP in the cuvette bind to the target antigen or
antibody. When the system exposes the cuvette to a magnetic field, the magnets
draw the PMP bound to antigen or antibody toward them. While the magnets hold
the PMP in place, the system washes away sample and reagent not bound to the
coated PMP.
1 PMP
2 Antibody
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Sandwich Format
Sandwich formats use AE-labeled antibody in the Lite Reagent.
1. The system adds Lite Reagent, containing AE-labeled antibody, to the sample.
The AE-labeled antibody binds specifically to the analyte-specific antigen
in the sample.
2. The system adds Solid Phase containing PMP that are coated with antibody
specific for the antigen in the sample.
The system incubates the cuvette at 37°C (98.6°F). PMP bind to the antigens
that are bound to AE-labeled antibody.
1 PMP-Antibody-Antigen-Antibody-AE complex
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3. The cuvette is exposed to a magnetic field, which draws PMP toward the
magnets.
While the magnets hold PMP in place, sample and reagent not bound to PMP
are washed away. The cuvette now contains AE bound to antigen, which is
bound to PMP by antibody.
1 Magnets
2 PMP-Antibody-Antigen-Antibody-AE complex
3 Cuvette
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4. The System adds acid and base to initiate the chemiluminescent reaction.
The system measures the emission of light in relative light units (RLUs).
When the system quantitates the light produced from the oxidation of AE, the
system calculates the concentration of antigen.
In a sandwich format, the analyte-specific antigen concentration in the
sample and the light emission in RLUs have a direct relationship. If more
analyte-specific antigen molecules are present in the sample, then more AE
is present, and therefore light emission is greater.
Competitive Format
Competitive formats include:
• AE-labeled antigen in the Lite Reagent
• AE-labeled antibody in the Lite Reagent
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AE-labeled Antigen
1. The system adds Lite Reagent, containing AE-labeled antigen, and Solid
Phase, containing antibody-labeled PMP, to the sample.
The system incubates the cuvette at 37°C (98.6°F). The AE-labeled antigen
competes with analyte-specific antigen in the sample for limited binding sites
on the antibody, which is bound to PMP.
If more analyte-specific antigen is present in the sample, then less AE-labeled
antigen is bound. Alternatively, if less analyte-specific antigen in the sample
is present, then more AE-labeled antigen is bound.
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2. The system exposes the cuvette to a magnetic field, which draws PMP toward
the magnets.
While the magnets hold PMP in place, the system washes away sample and
reagent not bound to PMP. The cuvette now contains analyte-specific antigen
from the sample and AE-labeled antigen bound to PMP by antibody.
1 PMP-Antibody-Antigen complex
2 PMP-Antibody-Antigen-AE complex
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3. The system adds Acid and base to initiate the chemiluminescent reaction.
The system measures the emission of light in RLUs. When the system
quantiates the light produced from the oxidation of AE, the system calculates
the concentration of the analyte.
If the sample has a low concentration of analyte-specific antigen, most
binding sites on the antibody are bound to AE-labeled antigen. This causes an
elevated reading of RLUs from the oxidation of AE.
If the sample has a high concentration of analyte-specific antigen, most
binding sites on the antibody are bound to antigen from the sample, and few
sites are bound to AE-labeled antigen. This causes a lower reading of RLUs
from the oxidation
of the AE.
In a competitive assay with AE-labeled antigen, the concentration of antigen
in the sample and the light emission in RLUs have an inverse relationship.
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AE-labeled Antibody
1. The system adds Lite Reagent, containing AE-labeled antibody, and Solid
Phase, containing antigen-labeled PMP, to the sample.
The system incubates the cuvette at 37°C (98.6°F). Antigen bound to PMP
competes with analyte-specific antigen in the sample for limited binding sites
on AE-labeled antibody.
If the sample has more analyte-specific antigen, then it binds less
PMP-labeled antigen. Alternatively, if the sample has less analyte-specific
antigen, then it binds more PMP-labeled antigen.
1 AE-labeled antibody
2 PMP-labeled antigen
3 Analyte-specific antigen in the sample
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2. The system exposes the cuvette to a magnetic field, which draws PMP toward
the magnets.
While the magnets hold PMP in place, the system washes away sample
containing analyte-specific antigen and reagent not bound to PMP.
The cuvette now contains PMP-antigen bound to AE by antibody.
1 PMP-Antigen-Antibody-AE complex
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3. The system adds acid and base to initiate the chemiluminescent reaction.
The system measures the emission of light in RLUs. When the system
quantitates the light produced from the oxidation of AE, the system calculates
the concentration of analyte.
If the sample has a low concentration of analyte-specific antigen, the
PMP-labeled antigen binds to most binding sites on the antibody. This
causes an elevated reading of RLUs from the oxidation of AE.
If the sample has a high concentration of analyte-specific antigen, the antigen
from the sample binds to most binding sites on the antibody, and few sites are
bound to PMP-labeled antigen. This causes a lower reading of RLUs from the
oxidation of AE.
In a competitive assay with AE-labeled antibody, the concentration of analyte
in the sample and the light emission in RLUs have an inverse relationship.
Antibody-capture Format
When the substance being measured in the sample is an antibody, the system uses
the antibody-capture format. The assay uses a reagent containing an additional
antibody that is specifically directed against the antibody in the sample.
Antibody-capture formats are usually characterized by 2 cycles of incubation and
wash. The purpose of the first incubation and wash is to remove excess interfering
substances in the sample. The purpose of the second incubation and wash is to
measure the antibody in the sample.
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2. The system exposes the cuvette to a magnetic field, which draws PMP toward
the magnets.
While the magnets hold PMP in place, the system washes away sample and
reagent not bound to PMP. The cuvette now contains IgM antibody from the
sample captured by specific anti-human IgM antibody, which is bound to
PMP.
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3. The system adds Lite Reagent, containing AE-labeled antigen, to the cuvette
and incubates it at 37°C (98.6°F).
The AE-labeled antigen binds to the sample IgM antibody in the sample.
1 AE-labeled antigen
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4. The system exposes the cuvette to a magnetic field, which draws PMP toward
the magnets.
While the magnets hold PMP in place, the system washes away the reagent
not bound to PMP. The cuvette now contains AE-labeled antigen bound to
sample IgM antibody, which is bound to anti-human IgM antibody, bound to
PMP.
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5. The system adds acid and base to initiate the chemiluminescent reaction. The
system measures the emission of light in RLUs. When the system quantitates
light produced from the oxidation of the AE, the system calculates the
concentration of the analyte.
In this example of an antibody-capture assay, the sample concentration and
the light emission have a direct relationship. If more sample IgM antibody is
present, then more AE is present, and therefore the light emission is higher.
References
1. Whitehead TP, Kricka J, Carter JN, Thorpe G. Analytical luminescence: its
potential in the clinical laboratory. Clin Chem 1979; 25/9: 1531–1546.
2. Woodhead JS, Campbell AK, McCapra F, Beheshti I, Weeks I. Acridinium
esters as high specific-activity labels in immunoassays. Clin Chem 1983; 29/
8: 1474–79.
3. Weeks I, Woodhead JS. Chemiluminescence immunoassays. J. Clin
Immunoassay 1984; 7/1: 82–89.
4. Patel A, Morton MS, Woodhead JS, Ryall MET, McCapra F, Campbell AK. A
new chemiluminescent label for use in immunoassay. Biochem Soc Trans
1982;
10: 224–5.
5. Tietz NW. Fundamentals of clinical chemistry, 3rd ed. Philadelphia: W.B.
Saunders Company, 1987; 1010.
6. Kricka LJ. Chemiluminescence immunoassay. The immunoassay handbook,
1st ed. New York: Stockton Press, 1994; 341–343.
7. Dudley R. The immunoassay handbook, 1st ed. New York: Stockton Press,
1994; 161–164.
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You can load samples on to the system at any time, even while the system
processes samples. The inprocess queue holds up to 16 racks. Before loading
samples ensure that you have met the following conditions:
• You are using defined tube types
Refer to Specifications‚ page E-1.
• You configured the sample racks for the tube types you are loading.
Refer to Specifying Sample Tube Types‚ page 8-5.
• Requests are available for the samples you load.
Refer to Managing the Worklist‚ page 2-39.
• You have sufficient sample volume.
Refer to Appendix C, Sample Volume Requirements‚ page C-12.
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Loading Samples
BIOHAZARD
Wear personal protective equipment. Use universal precautions. Refer to Safety
Instructions‚ page A-1 for recommended precautions when working with
biohazardous materials.
1. If you use sample cups, label them with the appropriate barcode labels and
dispense the sample into the labeled sample cups.
1 Barcode label
CAUTION
Do not manually push a rack into the inprocess queue and do not manually place
sample racks near the entrance to the inprocess queue without using the raised
area of the sample entry queue as a positioning guide. Failure to follow this
instruction can lead to a misread of the sample tube positions in the rack.
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CAUTION
Do not press the sample start button until you close the primary reagent door and
ensure that all reagent barcodes were read correctly. Starting the system before all
reagent barcodes are read correctly can cause erroneous results. Closing the
primary reagent door allows the system barcode scanner to rescan the reagent
barcodes and acknowledge any changes.
8. If the LED above the Sample Start button is not lit, press the button.
The system moves the rack to the inprocess queue. Refer to Verifying Barcode
Scanning‚ page 2-6.
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NOTE: If you remove the Tube-Type Selector, it is the same as moving the
Tube-Type Selector to position 1.
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NOTE: If the system cannot read a rack barcode, the system does not test the
samples, the rack field at the Status – Samples window is empty, and the
system ejects the rack.
3. Enter a specific search criterion in Search for.
• If the system did not read the barcode, the No Barcode message displays
in the Status column and the system does not test the sample.
• If the SID for the tube with the No Barcode status is available (for
example, from a sample loading list) manually enter the tube barcode.
4. To manually enter the SID, select the sample and then select Enter SID.
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NOTE: If you select Now, the system may not eject the sample rack
immediately. The system schedules the rack for ejection and ejects the rack
during the next available cycle. This may cause a slight delay before the
system ejects the sample rack into the sample exit queue.
If sampling stopped due to a problem with the sample tip loader, sample probe, or
a fluid sensor, remove the sample racks from the inprocess queue using diagnostic
tools. Refer to Using Diagnostic Tools.
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NOTE: If you select the Exit Queue button at the Worklist – Summary
window with a specific sample or test selected in the worklist table, the
Status – Exit Queue window opens and displays the sample rack position.
NOTE: If you select the Exit Queue button at the Worklist – Summary
window with no sample or test selected in the worklist table, the Status – Exit
Queue window opens with no sample rack position selected.
Monitoring Status
The system tracks the status of supplies, samples, QC exceptions, reagents,
maintenance, and events and alerts you if a system area needs attention. Using the
status buttons on the workspace, you can monitor the status of the different system
areas.
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System Status
Use System Status to monitor the condition of the system and determine which
area of the system needs attention.
To open the System Status window, at the workspace select the System Status
button and then select System Status in the menu.
Do not allow the system to remain in the Inprocess state or the Check Status state
for longer than 2 hours without processing samples. Refer to Managing
Nonsampling System Status‚ page 6-8.
• Areas change color to indicate they require your attention. Select the area to
obtain more detailed information.
Select the area to obtain more detailed information. For example, if the
cleaning solution is low, the color of the Supplies Status button and the
cleaning solution area changes to yellow. If an error occurs in the cuvette
loading area that causes the system to stop, the Event Log button and the
cuvette loading area change to red.
• When you position the pointer over an area, the system displays the name of
the area.
• You can select a button to obtain more detailed information on the status of
supplies, reagents, samples, maintenance, and the various subsystems.
Supplies Status
Use Supplies Status to monitor the level of system supplies. At the workspace,
the Supplies Status button changes color to indicate the status of system supplies:
• Yellow indicates that the system is using the reserve.
The main supply is removed, the supply is depleted, or the waste is more than
80% full or using the reserve.
• Red indicates that a supply and its reserve are depleted, the waste and its
reserve are full, or an error condition occurs.
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Select the Supplies Status button to open the Status – Supplies window and
determine which supply needs attention. The system does not start if a supply
status is yellow. The system stops processing samples if a supply status is red.
The system displays status messages in the field below the supply name. For a
description of the status messages for each supply and the procedure for
replenishing the supply, move the pointer to the supply field and select the
help (?) button.
The system displays these messages on the Status – Supplies window:
Supply Area Message Color Description
Cuvettes OK Neutral At least 1 cuvette is available.
No Cuvette Red The cuvette supply is depleted or an
Detected error in cuvette loading occurred and the
system stopped sampling.
Tips OK Neutral At least 120 sample tips are available.
<120 Tips Yellow Less than 120 sample tips are available.
Depleted Red No sample tips are available and the
system stopped sampling.
Cuvette Waste OK Neutral The bin is less than 80% full.
>80% Full Yellow The bin is more than 80% full.
Not Present Yellow The bin was removed. The system is
using the reserve.
Sampling Red The system stopped sampling because
Stopped the cuvette bin is full.
Full Red The bin is full.
Tip Waste OK Neutral The bin is less than 80% full.
>80% Full Yellow The bin is more than 80% full.
Not Present Yellow The bin was removed. The system is
using the reserve.
Sampling Red The system stopped sampling because
Stopped the tip bin is full.
Full Red The bin is full.
Tip Tray Waste OK Neutral The waste area is less than 80% full.
>80% Full Yellow The waste area is more than 80% full.
Sampling Red The system stopped sampling because
Stopped the waste area is full and the current tip
tray is empty.
Liquid Waste OK Neutral The bottle is present and has enough
space to continue testing.
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Sample Status
Use Sample Status to monitor the samples that are loaded on the system. At the
workspace, the Sample Status button changes color to indicate the status
of samples that are in the inprocess queue and the lab automation queue:
• Yellow indicates that testing for a sample is not complete because of a
problem with a sample.
• Red indicates that testing for all samples in a sample rack is not complete
because of a problem with the sample rack barcode label.
Select the Sample Status button to open the Status – Samples window and review
the status of the samples in the inprocess queue and the lab automation queue.
NOTE: The Lab Automation Queue view is only available when lab automation
is enabled. Refer to Defining LAS Communication Options‚ page 8-47.
The following messages indicate the status of a sample:
Message Color Description
Aspirated Neutral All tests for this sample are aspirated.
Clot Yellow The system detected a clot in the.sample probe. Check
Detected the sample for clots.
Incomplete Yellow One of the calibrator levels is not loaded on the system.
Cal Set Ensure that both the high calibrator and the low calibrator
are loaded.
Incomplete Yellow The complete set of controls for this control-bracketed test
Set is not loaded on the system in the same rack.
This message can apply to start-of-bracket or
end-of-bracket controls. Ensure that all of the controls
specified at the Test – Definition window are loaded on the
system in 1 rack.
Insufficient Yellow Not enough sample volume exists for the requested tests.
Integrity Yellow The system detected an irregular sample aspiration.
Error Check the sample for bubbles or foam, or check the
sample probe tubing and tip-probe connection for leaks.
When you perform multiple tests on a patient sample, if
the sample produces an integrity error, all tests for that
sample are flagged with the integrity error flag.
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Exception Status
Selecting this button displays a message directing you to the applications view
and ADVIA QC.
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Reagent Status
Use Reagent Status to monitor the status of primary and ancillary reagents on
the system. The Reagent Status button changes to yellow to indicate that a reagent
pack cannot be used.
Select the Reagent Status button to open the status window of the reagent that
requires attention or to open the Worklist – Reagent Inventory window.
If . . . Then . . .
a primary reagent needs attention or if no the system displays the Status – Primary
reagent needs attention, Reagent window.
an ancillary reagent needs attention the system displays the Status – Ancillary
Reagent window
a reagent pack is not onboard or is below the system displays the
the defined reagent warning counts for Worklist – Reagent Inventory window
tests requested in the current worklist
a primary reagent and an ancillary reagent the system displays the
need attention Status – Primary Reagent window and the
Status – Ancillary Reagent window
a primary reagent needs attention and a the system displays the
reagent pack is not onboard or is below Status – Primary Reagent window and the
the defined reagent warning counts for Worklist – Reagent Inventory window
tests requested in the current worklist
an ancillary reagent needs attention and a the system displays the
reagent pack is not onboard or is below Status – Ancillary Reagent window and
the defined reagent warning counts for the Worklist – Reagent Inventory window
tests requested in the current worklist
a primary reagent and an ancillary reagent the system displays the
need attention, and a reagent pack is not Status – Primary Reagent window, the
onboard or is below the defined reagent Status – Ancillary Reagent window, and
warning counts for tests requested in the the Worklist – Reagent Inventory window
current worklist
Primary Reagents
If a primary reagent requires attention or if no reagent requires attention when you
select the Reagent Status button, the system displays the Status – Primary reagent
window.
• Pack indicators change color to indicate if a pack is available for use.
The numbers on the pack indicators and the table correspond to the spaces in
the primary reagent compartment.
• The system displays Status messages for each reagent pack that requires
attention.
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Ancillary Reagents
If an ancillary reagent requires attention and you select the Reagent Status button,
the Status – Ancillary Reagent window is displayed.
The following messages indicate the status of a reagent:
Message Color Description
Depleted Yellow The reagent pack is empty.
Lot Expired Yellow The reagent lot expired. Load a new lot of reagent.
No Test Yellow No test definition exists for the test.
Definition
Removal Neutral You requested to remove this pack. After the system
Pending completes the tests scheduled for this pack, the system
ejects the pack.
Stability Yellow The reagent pack exceeded the stability date.
Expired
Verify Neutral The reagent pack exceeded the stability date. The system
Stability uses the reagent for testing because you selected Use
Beyond for this test at the Test – Definition window.
Maintenance Status
Use Maintenance Status to monitor maintenance activities and automated
procedures. At the workspace, the Maintenance Status button changes color
to indicate the status of maintenance:
• Yellow indicates that a maintenance activity is due or overdue.
• Red indicates that an automated maintenance procedure did not complete.
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Select the Maintenance Status button to display the Status – Maintenance window
and display the procedures that require attention.
• In the Schedule view, the date column displays the date the activity is due.
• In the Log view, the date column displays the date the activity was completed.
The ADVIA Centaur XP system monitors maintenance activities and notifies you
when a scheduled activity is due. The system provides a maintenance schedule for
you to record the completion of scheduled maintenance activities. The system
then uses this information to automatically update the maintenance schedule with
the next time the activity is due. Refer to Defining Maintenance Activities‚
page 5-3.
Replenishing Reagents
You manually mix the primary packs and load them on the reagent holders in the
primary reagent compartment, which stores the reagents at 4 to 8°C (39 to 46°F).
The primary reagent shelf mixes the packs to keep the reagents in suspension. The
arrow indicator and color on each pack indicate where you can load the packs to
optimize sample throughput. Refer to Appendix C, Handling Reagents, in the
ADVIA Centaur Assay Manual.
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Each ancillary pack contains 1 ancillary reagent. The system tracks the volume in
the pack. Refer to the ADVIA Centaur Assay Manual. Some ancillary packs are
customer-fillable packs.
Loading Reagents
You can monitor the volumes of primary and ancillary reagents using the
Worklist – Reagent Inventory window. To open the Worklist – Reagent Inventory
window from the workspace, perform the following steps:
1. Select Worklist.
2. Select Reagent Inventory.
The Worklist – Reagent Inventory window fields display reagent information:
• The Available column displays the total amount of shared reagent that is
onboard and available.
• The Shortage column displays the amount of reagent needed to complete the
scheduled tests.
• The Status column displays the primary reagent status and the ancillary
reagent status.
Inventory for primary reagents is expressed in number of tests. Inventory for
ancillary reagents, diluents, and ancillary wash solutions is expressed in
milliliters.
NOTE: Do not add reagent to reagent packs. Each reagent pack contains enough
reagent to process a predetermined number of tests. Each time the system accesses
a reagent pack, reagent volume inventory tracks the amount of reagent used. The
system does not recognize additional fluid placed in a reagent pack.
If you have multiple systems in your laboratory, record the following information
before you move a reagent pack from 1 system to another:
• the number of tests remaining in a primary reagent pack
• the stability information that displays at the Status – Primary Pack Details
window
• the volume remaining in an ancillary reagent pack
• the stability information that displays at the Status – Ancillary Pack Details
window
Enter this information in the appropriate fields at the Status – Primary Pack
Details window and the Status – Ancillary Pack Details window when you load
the reagents on a different system.
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You can select a pack indicator to display information about the reagent pack in
that position. The colors underneath the pack indicator denote the type of pack
loaded in that pack position. The number in the Status column indicates the
number of tests available in the pack.
CAUTION
Do not press the sample start button until you close the primary reagent door and
ensure that all reagent barcodes were read correctly. Starting the system before all
reagent barcodes are read correctly can cause erroneous results. Closing the
primary reagent door allows the system barcode scanner to rescan the reagent
barcodes and acknowledge any changes.
3. Press the sample start button for the system barcode scanner to rescan the
reagent barcodes and acknowledge any reagent changes.
Refer to Appendix C, Handling Reagents, in the ADVIA Centaur XP Assay
Manual.
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CAUTION
Do not remove a primary pack when the system is inprocess if the LED on the
primary reagent door is green. Removing a primary pack when the system is
inprocess and the LED is green can cause erroneous results and mechanical
damage to the system. Wait until the LED is neutral before you remove the
primary pack.
When the status changes to Removable and the LED changes to neutral, remove
the reagent pack.
1 LEDs
2 Ancillary reagent entry
3 Ancillary pack displaying barcode
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Replenishing Supplies
You can select the Supplies Status button at the workspace to open the Status –
Supplies window and review the status of supplies. Refer to Supplies Status‚
page 2-10.
You can also move the pointer to the supply field and press the help (?) button
on the pointing device for a description of the status messages for each supply
and the procedure for replenishing each supply.
The system has the following capacity for supplies:
Supply Capacity
Cuvettes 1000 cuvettes
Tips 840 tips
Cuvette Waste 700 cuvettes
Tip Tray Waste 8 trays
Tip Waste 1000 tips
Liquid Waste 7.5 L
Water 10.0 L
Acid 1500 mL
Base 1500 mL
Wash 1 2500 mL
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Supply Capacity
Wash 3 2500 mL
Cleaning Solution 2100 mL
CAUTION
Do not grasp the tubing when disconnecting the tubing from the top of the
water bottle or reservoir. Pulling the tubing can cause crimps. Grasp the
fittings, not the tubing.
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c. Disconnect the water tubing from the water bottle and then set the tubing
on lint-free tissues or gauze to absorb drips.
d. Lift the water bottle up and out.
2. Remove the cap.
3. Fill the spare, clean water bottle with fresh reagent water and install the cap.
Refer to Reagent Water Quality‚ page C-4.
4. Install the spare, full water bottle:
a. Place the water bottle in the waste and water drawer.
b. Connect the water bottle tubing to the bottle.
A blue spiral wrap designates this tubing.
c. Lower the water bottle lid.
d. Push in the waste and water drawer.
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1 Sensor tip
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2. Insert the Waste Bottle Stopper into the sensor and tubing openings.
1 Waste bottle
2 Sensor clamp
3 Sensor opening
4 Waste bottle stopper
5 Tubing opening
3. Remove the waste bottle from the waste and water drawer.
4. Empty the waste bottle:
a. At the location in your laboratory designated for waste disposal, loosen
the waste bottle cap.
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c. Close the waste bottle valve, ensuring that the waste bottle valve is closed
completely.
d. Tighten the waste bottle cap.
5. Replace the waste bottle:
a. Install the waste bottle in the drawer.
b. Remove the waste bottle stopper.
c. Clean the stopper using lint-free tissues or gauze and cleaning solution.
d. Replace the stopper under the waste bottle handle.
Refer to Attaching the Waste Bottle Stopper‚ page 2-30.
e. Install the sensor and move the clamp to cover the sensor.
f. Connect the tubing to the waste bottle.
An orange spiral wrap designates this tubing.
g. Lower the waste bottle lid.
6. Push in the waste and water drawer.
7. When prompted, verify the waste bottle tubing is reconnected.
8. Select Continue.
9. Close the waste and water drawer.
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3. Thread the cable tie through the hole on the Waste Bottle stopper.
4. Wrap the cable tie around the handle of the waste bottle.
5. Insert the tip of the tie into the cable tie lock.
Adjust the tie as necessary. Do not secure the cable tie too tight. The stopper
needs to move freely around the handle and be able to reach the tubing and
sensor openings.
NOTE: The cable tie cannot be adjusted after you lock it.
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WARNING
Do not handle cleaning solution without wearing protective equipment, including
gloves, laboratory coat, and safety glasses or protective face shield.
1. Open the sample tip waste area door.
2. Remove the cleaning solution bottle:
a. Pull out the drawer and the cleaning solution bottle.
b. Lift the lid.
1 Lid
2 Sample tip waste area door
3 Cleaning solution bottle
4 Drawer
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Adding Cuvettes
You can fill the cuvette loading bin any time, even while the system processes
samples.
1. Open the supplies access cover.
CAUTION
Do not leave the top cover open when you add cuvettes to the cuvette loading bin.
Leaving the top cover open allows cuvettes to fall into another part of the system
where it may obstruct normal operation. Close the top cover when adding cuvettes
to the cuvette loading bin.
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3. Install the sample tip trays, ensuring that the tab is facing you and that the
notches in the trays are on the right.
4. Pull the tab to release the strap from the trays and then remove the strap.
5. Close the supplies access cover.
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BIOHAZARD
Wear personal protective equipment. Use universal precautions. Refer to Safety
Instructions‚ page A-1 for recommended precautions when working with
biohazardous materials.
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BIOHAZARD
Wear personal protective equipment. Use universal precautions. Refer to Safety
Instructions‚ page A-1 for recommended precautions when working with
biohazardous materials.
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4. Dispose of the contents of the bin into a container approved for biohazardous
waste.
NOTE: The liners for the cuvette waste bin and the sample tip waste bin are
not interchangeable. Ensure that the cuvette waste bin liner is installed in the
cuvette waste bin.
5. If the liner is damaged, install a new liner.
6. Install the cuvette waste bin, ensuring that the bin is pushed in completely.
7. Close the cuvette waste area door.
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You can replace the system fluids any time, even while the system processes
samples. The system fluid reservoirs hold enough fluid to allow the system to
complete the contents of the incubation ring.
WARNING
Do not allow contact of Acid and Base reagents with skin and eyes. Acid and Base
reagents are irritating to the skin. Wear suitable eye, face, and skin protection,
which include wearing gloves, protective eye shield, and laboratory coat. In case
of contact with the eyes, rinse the eyes immediately with plenty of water and seek
medical advice. In case of accident or if you feel unwell, seek medical advice. If
possible, show the bottle label and the Material Safety Data Sheet. In case of
accidental ingestion of reagent, call a physician immediately.
NOTE: Replace Acid and Base reagents at the same time to manage lot numbers
and to obtain optimum reagent performance.
1. Pull the system fluid drawer towards you.
CAUTION
Do not touch the tubing or sensors. Touching the tubing or sensors can cause
contamination.
CAUTION
Dispose of Acid and Base reagents in accordance with local, state, and federal
regulations. Refer to the Material Safety Data Sheets for each material for
additional information.
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3. Remove a bottle and discard the fluid into an approved fluid waste container
and discard the bottle into an approved solid waste container.
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The following table describes the View and Sort combinations you can use to
perform some routine tasks:
Task View Sort Results
Display the list of all current requests Current SID Conc or
Index
Display batch requests as a batch, before Pending Scanned NA*
the ending rack is scanned Batches
Display sample results, not all tests are Current SID Conc or
complete Results Index
Display samples that have tests that are Pending SID NA*
scheduled or inprocess, but not complete Tests
Display Stat sample results Stats SID Conc or
Index
Display sample results, all tests Historical SID Conc or
are complete Index
Display calibrator results Calibrators SID Conc or
Index
Display samples with tests that have RLU Stored SID RLU
values, but not a calculated concentration RLUs
Display a list of patient results that have Historical PID Conc or
been moved to the historical worklist. Index
Display the list of all current tests for a Current PID Conc or
patient Index
Display a list of samples that need to Waiting for SID NA*
process an end-of-bracket set of controls Controls
before the system reports the results
NOTE: This view applies to
control-bracketed tests only. The Repeat
and Release buttons are unavailable in this
view because the results are retained until
the end-of-bracket controls are processed
and acceptable.
Display a list of samples with discarded Results SID NA*
test results Discarded
For more information on discarded test
results, refer to Reasons for Discarded
Results‚ page 2-43.
NOTE: This view applies to
control-bracketed tests only. The Release
button is unavailable in this view because
the results have already been discarded.
NA = Not Applicable
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A dilution is scheduled for the test. If this symbol displays on the left side
of the worklist, it indicates a manual dilution. If this symbol displays on
the right side of the worklist, it indicates an automatic dilution.
A comment is entered.
In the Worklist, this symbol means the sample has been ejected from the
inprocess queue. At the Status – Exit Queue window, this symbol means
the sample was scheduled by rack and it has no SID.
The system scheduled a repeat for the test or the result was obtained when
the test was repeated.
On a button, this symbol means the system opens a window when you
select the button. In a table, this symbol means the result has multiple
flags.
The result is on hold so that it can be reviewed before it is released.
In Worklist, this symbol means you changed the number of replicates for
the test or specified a particular reagent lot or pack for the test. In
Maintenance, this symbol indicates a maintenance procedure you defined
for your laboratory.
The test is due to complete at the time displaying to the left of this symbol.
Tests for this sample did not complete. Reschedule the tests and reload
the sample.
This symbol displays only for control-bracketed tests. It represents a
Waiting for Controls test, which is a test that needs an end-of-bracket set
of controls to be completed before the system reports the result.
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Symbol Description
This symbol displays only for control-bracketed tests. It represents a
Results Discarded test. The test result was discarded for 1 or more of the
following reasons:
An open bracket is a control-bracketed test that has acceptable
start-of-bracket control results but the end-of-bracket controls have not
been processed.
The system did not process the end-of-bracket controls within the
acceptable time interval.
At least 1 of the end-of-bracket control results was unacceptable.
The system aspirated a calibrator before aspirating the end-of-bracket set
of controls for an open bracket.
The calibration expired before the system processed the end-of-bracket set
of controls for an open bracket.
The reagent lot expired before the system processed the end-of-bracket set
of controls for an open bracket.
A field service provider performed controller diagnostics before the
system processed the end-of-bracket set of controls for an open bracket.
A field or setting for the control-bracketed test was modified at the
Test – Definition window before the system processed the end-of-bracket
set of controls for an open bracket.
You performed 1 of the following functions at the
Setup – Data Administration window before the system processed the
end-of-bracket set of controls for an open bracket:
Test Definition, Update
Test Definition, Restore
Reagent Inventory, Restore
Calibration Data, Restore
Quality Control Data, Restore
Worklist, Delete
CAUTION
Use caution when deleting a worklist with unreleased results. When you delete a
worklist with unreleased results, the system permanently removes the associated
unreleased results from the database. If the unreleased results are from an open
bracket, the associated samples do not display in the Results Discarded report
generated at the Print – Report Options window or the Results Discarded view at
the Worklist – Summary window.
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Message Description
Invalid The calibration exceeds either the defined or the observed range for 1 or
more calibration expected values. Schedule another calibration at the
Worklist – Schedule window.
Verify The calibration is outside the observed range but within the defined range
and an operator accepted the calibration. Schedule another calibration at
the Worklist – Schedule window.
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CAUTION
Do not press the sample start button until you close the primary reagent door and
ensure that all reagent barcodes were read correctly. Starting the system before all
reagent barcodes are read correctly can cause erroneous results. Closing the
primary reagent door allows the system barcode scanner to rescan the reagent
barcodes and acknowledge any changes.
2. If the LED above the Sample Start button is not lit, press the button.
The system moves the rack to the inprocess queue.
3. If you schedule by SID and the sample tube does not have a barcode label,
you can enter the SID after the sample is scanned:
a. At the workspace, select the Sample Status button.
b. At the Status – Samples window, select the sample with a No Barcode
status and then select Enter SID.
c. At the Status – Enter SID window, enter the SID.
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You can enter the SID only while the sample is in the Inprocess Queue.
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CAUTION
For most sample processing, do not schedule by Rack ID. If, however, it is
necessary to schedule a sample by rack ID, be aware that the system ejects the
sample to the exit queue after aspirating the sample. Reflex or other testing cannot
complete once the system ejects the sample to the exit queue. To obtain final
results for reflex or other testing, reintroduce the sample rack to the front of the
sample entry queue.
If you are scheduling samples for a control-bracketed test, you must schedule
by SID. The system does not allow you to schedule samples for
control-bracketed tests by Rack.
4. Select how you want the system to identify the sample:
• If the sample identifier selected at the Setup – Tube Type & Barcode
window is SID, you can select Schedule by SID or Schedule by Rack.
Refer to Specifying Sample Identification‚ page 8-4.
• If the sample identifier selected at the Setup – Tube Type & Barcode
window is Rack, you can only select Schedule by Rack.
Refer to Scheduling Non-barcoded Samples by Rack ID‚ page 2-50 and
Scheduling Barcoded Samples by Rack ID‚ page 2-51.
NOTE: After entering the SID or the Rack ID, you must press Enter on the
keyboard. If you do not press Enter after entering the SID or the Rack ID, the
test selection table and the functions at the bottom of the window do not
become active.
5. Enter the SID or the Rack ID and then press Enter.
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• You can enter up to 13 alphanumeric characters for the SID. Do not use
the % symbol as part of an SID.
• The Rack ID consists of 4 numbers (0001 – 9999) followed by a letter
(A – E).
Enter the letter in uppercase.
6. If you want to process the sample before routine samples, select Stat.
7. Select or enter the tests or the profiles for the sample.
If you use the keyboard to enter the tests or profiles in the Test field or the
Profile field, you must enter the test name or profile exactly as it displays on
the schedule window.
8. Select Save.
You can select Summary to view the requests in the worklist.
NOTE: If you have groups of samples with the same tests, scheduling the
tests in batch is the most efficient method of scheduling without using an LIS.
Refer to Scheduling Batches‚ page 2-54 for more information.
Scheduling by Rack ID
NOTE: Move results to historical before reusing a rack for samples scheduled by
Rack ID. Moving results to historical prevents merging new results with current
results when scheduling samples by rack ID.
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The system functions similarly when the LIS schedules samples by rack ID.
• If the system has not scanned the rack, the system recalls the existing request
for that rack position and merges additional tests to that request.
• If the system has scanned the rack or the rack is not in the current worklist, the
system creates a new worklist request for the rack position.
When a sample is scheduled by rack ID, the system does not use the SID to
process that sample even if the tube has a barcode label. In some cases, if the
system scans a barcode label for that sample tube, the system enters the SID from
the barcode label to the worklist request for the rack position. This provides a
check to identify a possible conflict between the SID in the worklist and the SID
on the sample tube barcode label. When the system identifies a possible conflict, it
alerts the operator by displaying the SID Mismatch sample status message.
The following table contains information about how the system uses the SID for
samples scheduled by rack ID:
Did the system
previously scan
the rack position
that is in the Worklist
current worklist? SID Barcode Label System Action
no not present present makes the SID part of the
rack ID in the worklist
request
no present present, different displays the SID Mismatch
from worklist message; possibly incorrect
SID tube
yes, with no barcode not present present displays the SID Mismatch
label on the tube message; possibly incorrect
tube
yes, with no barcode present not present assumes that the sample is the
label on the tube same tube and performs
pending or new tests
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Scheduling Batches
When the LIS is not operational or not connected, the most efficient method of
scheduling tests is to schedule them in batch at the Worklist – Schedule window.
The system performs the requested tests on all samples loaded in the starting rack,
ending rack, and all racks in between.
1. At the workspace, select Worklist.
2. Select Schedule.
3. At the Worklist – Schedule window, select Batch.
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5. Select Continue.
6. At the Worklist – Schedule window, select Save.
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3. Select Continue.
4. At the Worklist – Schedule window, select Save.
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You do not need to enter the rack ID and position of the controls. You can use the
same rack for the start-of-bracket controls and end-of-bracket controls as long as
the system processed the previous set of controls with acceptable results and
ejected the rack.
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NOTE: If you select samples on the left side of the worklist summary table, all
tests for the sample are selected.
If you start selecting on the left side of the worklist summary table, the system
assumes that you want to select samples even if your last selection at the time is
on the right side of the table. The system selects all tests for the samples in the
selected rows.
If you select tests on the right side of the worklist summary table, you can select
specific tests for a sample.
If you start selecting on the right side of the worklist summary table, the system
assumes that you want to select tests even if your last selection at the time is on
the left side of the table. The system selects only tests in the selected rows.
If you need to scroll to continue selecting multiple samples or tests, release the
Shift or Ctrl key, scroll to the desired location, and then again hold down the Shift
or Ctrl key while selecting the sample or test.
When you select multiple tests and then select the Delete button, the following
information applies:
• The system cannot delete tests that are part of a ratio test and calibrators.
The system only deletes the tests that it can delete.
• The system can only delete a predetermined number of tests at a time.
If tests remain highlighted, select the Delete button again to delete more tests.
Editing Requests
Use this procedure to perform the following actions:
• add tests
• change how the system processes a sample (Stat or routine)
• change dilutions
• specify reagent lots or packs
• change the number of replicates
• change demographics
To edit worklist requests, perform the following steps:
1. At the workspace, select Worklist.
2. Select Summary.
3. At the Worklist – Summary window, select a sample or a test.
4. Select Schedule.
5. At the Worklist – Schedule window, edit the request.
6. Select Save to save the change.
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Deleting Requests
Use this procedure to delete selected samples or tests.
1. At the workspace, select Worklist.
2. Select Summary.
3. At the Worklist – Summary window, select a sample, a test, or a batch.
If you want to . . . Then . . .
delete a sample or a test, select a sample or a test.
delete a batch, select Pending Batches in View and then select a batch.
4. Select Delete.
5. Select Yes.
NOTE: You cannot delete calibrators that are resulted.
If tests have not started or if tests are complete, the entire request is deleted
immediately from the worklist.
If tests are inprocess, the tests are stopped, the cuvettes are discarded, and the
tests, including any completed results, are deleted from the worklist.
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NOTE: You can view a control-bracketed test result at the Worklist – Result
Details window when the system releases the result.
If the system has not released the result, the Worklist – Result Details window
displays the Waiting for Controls symbol.
If the system discarded the result, the window displays the Results Discarded
symbol.
Refer to System Symbols‚ page F-1.
If the control-bracketed test is waiting for controls or the results are discarded, the
Worklist – Result Details window does not display RLUs.
Result Flags
Flag Description
Above Check The result is above the check range entered in the test definition.
The test is repeated if you select Repeat if > for Check Range at
the Test – Ranges window.
Autorepeat The system automatically repeated this test as specified in the
test definition.
Below Check The result is below the check range entered in the test definition.
The test is repeated if Repeat if < is selected for Check Range at
the Test – Ranges window.
Cal Accepted The result was obtained using an operator-accepted calibration
for this test.
Cal Assigned The result was obtained after you assigned a valid calibration
for this test at the Worklist – Summary window.
Cal Invalid The calibration is invalid for this test. If an RLU is displayed for
the test and you have the appropriate level of security, you can
assign the previous valid calibration. At the
Worklist – Summary window, select the result and then select
Assign Cal.
Cal Required No valid calibration is available for the reagent lot. Schedule a
calibration at the Worklist – Schedule window.
Cancelled A test was cancelled and unable to complete because of a
conflict or resource problem while processing.
Check Variable A result was not obtained for the ratio test because of an error
flag for 1 or more of the ratio variables. Check the variables for
the ratio.
Clot Detected A result was not obtained because a clot was detected in this
sample.
> Conc Range The result is above the high value for the concentration
calculation defined at the Calibration – Master Curve Definition
window. The test is repeated if you select Repeat if > Conc
Range at the Test – Ranges window.
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Flag Description
< Conc Range The result is below the low value for the concentration
calculation defined at the Calibration – Master Curve Definition
window. The test is repeated if you select Repeat if < Conc
Range at the Test – Ranges window.
Conf N/A The system cannot calculate a result for a confirmatory test on
this sample.
Control bracketed The system processed this sample as part of a group of samples
for a specific test that began and ended with a set of controls.
The system does not process samples until it establishes the
acceptability of the results of the beginning set of controls. The
system does not report sample results until it establishes the
acceptability of the results of the ending set of controls.
The system displays this flag at the Worklist – Result Details
window and the Worklist – Summary window. If your system
transmits results to a laboratory information system (LIS), this
flag is transmitted also.
Control bracketed The system processed this sample with a control-bracketed test
restricted in the restricted mode.
A control-bracketed test is a test that requires evaluating a set of
controls at the beginning and end of a group of patient samples.
The system does not process samples until it establishes the
acceptability of the results of the beginning set of controls. The
system does not report sample results until it establishes the
acceptability of the results of the ending set of controls.
The restricted mode is a mode of processing in which the system
performs the same test on a group of samples loaded between
defined starting and ending racks without the interruption of any
other test or samples.
The system displays the Control bracketed restricted flag at the
Worklist – Result Details window and the Worklist – Summary
window. If your system transmits results to a laboratory
information system (LIS), this flag is transmitted also.
Diluted The result was obtained on a sample that the system
automatically diluted.
> Dilution Pt The result is above the dilution point defined for this test at the
Test – Ranges window.
Edited You modified the result that was reported by the system.
Entered You manually entered the test result (usually for an off-system
test).
Exception The quality control result caused a defined quality control rule
violation.
Excluded The quality control result is excluded from statistics or the
calibrator replicate is excluded from the calibration.
High The result is above the reference range (normal range) entered
in the test definition.
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Flag Description
High CV The CV (coefficient of variation) for replicates of the sample is
above the acceptable CV entered in the test definition.
Incomplete Cal Set One of the calibrator levels is not loaded on the system. Ensure
that both the high calibrator and the low calibrator are loaded.
In Check Range The result is equal to or above the defined low limit and equal to
or below the defined high limit of the range. The test is repeated
if the appropriate Repeat if criteria is selected for Check Range
at the Test – Ranges window.
> Index Range The result is above the high value for the index calculation
defined at the Calibration – Master Curve Definition window.
The test is repeated if Repeat if > Index is selected at the Test–
Ranges window.
< Index Range The result is below the low value for the index calculation
defined at the Calibration – Master Curve Definition window.
The test is repeated if Repeat if < Index is selected at the Test–
Ranges window.
Insufficient Not enough sample volume is available for the requested tests.
Integrity Error A result was not obtained because the system detected an
irregular sample aspiration. Check the sample for bubbles or
foam, or check the sample probe tubing and the tip-probe
connection for leaks.
Investigational This test is for investigational use only.
> Linearity The result is above the linearity you entered at the Test – Ranges
window. The test is repeated if Repeat if > is selected for
Linearity at the Test – Ranges window.
< Linearity The result is below the linearity you entered at the
Test – Ranges window. The test is repeated if Repeat if < is
selected for Linearity at the Test – Ranges window.
Lot Expired The reagent lot expired. Define a Master Curve for a new
reagent lot at the Calibration – Master Curve Definition
window.
Low The result is below the reference range (normal range) entered
in the test definition.
Low Acid Insufficient acid reagent is available on the system to perform
the test.
Low Base Insufficient base reagent is available on the system to perform
the test.
Low Tips Insufficient number of sample tips are available to perform the
test.
Low Wash 1 Insufficient wash 1 reagent is available on the system to perform
the test.
Low Wash 3 Insufficient wash 3 reagent is available on the system to perform
the test.
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Flag Description
Low Probe Wash Insufficient probe wash is available on the system to perform
the test.
Low Water Insufficient water is on the system to perform the test.
Manual Dilution A manual dilution was specified for this test result. The result is
automatically corrected for the specified dilution factor.
No Ancillary The test requires an ancillary reagent. Load the appropriate
ancillary reagent.
No Bracket The system cannot process this sample at this time because the
start-of-bracket controls were not processed or at least 1 of the
control results is unacceptable. The system will process the
sample after the start-of-bracket controls are processed and
produce acceptable results.
No Calculation A result was not obtained because a calculation error occurred.
For example, the RLUs for the sample are off the curve. For
more information, call for technical assistance.
No Cal Lot Match The test requires a specific primary reagent and calibrator lot
combination. Ensure that you use the primary reagent and the
calibrators from the same kit.
No Dil Allowed The system cannot calculate this result because a dilution is not
allowed for this test.
No Diluent An automatic dilution is scheduled and no diluent is loaded for
this test.
No Lot Match The test requires a specific primary reagent and ancillary
reagent lot combination. Ensure that you use the primary
reagent and the ancillary reagent from the same kit.
No Primary No primary reagent is loaded for this test.
If you are processing control-bracketed test and you get a No
Primary flag when you are not out of primary reagent, the
system is indicating that patient samples were not processed
because insufficient time remained to complete the bracket.
No sIgE Universal No sIgE universal reagent is onboard or the pack that is on the
system is empty.
No Vial Rgt No allergen reagent is loaded for this test.
Out The result for this control is above or below the limits entered in
the control definition. This flag does not display when quality
control rules are defined and enabled for a test.
Overdiluted A result was not obtained because the diluted sample result is
below the overdilution point defined at the Test–Ranges
window.
Priming The system cannot perform the test because the system needs to
schedule priming for a probe or a pump.
Redilute Dilute and repeat the test.
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Flag Description
repeated The system automatically repeated the test or you repeated the
test by selecting the test and then selecting the Repeat button at
the Worklist – Summary window or the
Worklist – Result Details window.
restricted The system processed this sample as part of a group of samples
for a specific test loaded between defined starting and ending
racks without the interruption of any other test or samples.
The system displays this flag only at the
Worklist – Result Details window. If your system transmits
results to a laboratory information system (LIS), this flag is
transmitted also.
Signal Error A result was not obtained because a signal error occurred. For
example, the RLUs for the sample are near zero, because of a
low light count to dark count ratio, or of noise. Contact your
local technical support provider or distributor.
System Error The system cannot perform the test because a subsystem is
offline.
Test Inactive The test is inactivated. At the Status – Primary Reagent window,
you can activate the test by selecting a pack for the test and then
selecting Activate Test. At the Status – Carrier Details window,
you can activate a specific IgE test when the system is not
inprocess by deselecting the Inactivate Test button for that test
vial.
Waste Full The system cannot perform the test because the cuvette bin, the
sample tip bin, the tip tray bin, or the waste bottle is full.
Wrong Pack ID Primary or ancillary reagent with the specified pack serial
number is not loaded for this test.
Wrong Vial ID Allergen reagent with the specified vial serial number is not
loaded for this test.
Waived The result was obtained using an operator waived calibration for
this test.
Repeating a Test
Use this procedure to manually schedule a repeat for a completed test. Refer to
Defining Automatic Repeats for Sample Error Conditions‚ page 8-27.
1. At the workspace, select Worklist.
2. Select Summary.
3. At the Worklist – Summary window, select a test on the right side of
the worklist.
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4. Select Repeat.
If . . . Then . . .
the sample is in the inprocess queue when the system automatically performs the
the repeat test is scheduled, repeat test on the sample.
the sample has been ejected from the you must reload the sample.
inprocess queue before the repeat test is
scheduled,
When the repeat completes, you can review the initial results and the repeated
results at the Worklist – Result Details window.
Editing Results
Use this procedure to change the concentration or index value of a result. You can
also use this procedure to enter the result for an off-system test. You must have the
appropriate level of security to edit results.
1. At the workspace, select Worklist.
2. Select Summary.
3. At the Worklist – Summary window, select a sample.
4. Select Result Details.
5. At the Worklist – Result Details window, select a result.
6. Select Edit Result.
7. At the Worklist – Edit Results window, edit the value:
• If the result is a concentration value, change or enter the value in Conc.
• If the result is an index value, change or enter the value in Index.
• If the result has both concentration and index values, both fields display at
the Worklist – Edit Results window; however, you can only edit the Conc
field.
8. Enter comments, as needed, to record why you edited the result:
a. Select Comments.
b. At the Comments window, enter a comment.
You can enter up to 3 comments. Comments can be up to 120
alphanumeric characters. The system enters your initials and the date.
If you are not currently signed in, you must sign in to the system.
c. Select Save.
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Use the following procedure to release all results on hold for the selected test:
1. At the workspace, select Worklist.
2. Select Summary.
3. At the Worklist – Summary, select the test with the hold status.
4. Select Release.
5. At the Worklist – Release Results window, select Release All Results on
Hold for the Selected Test and then select Continue.
The system transmits all results on hold to the LIS.
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Deleting Results
Use this procedure to delete results.
NOTE: Before deleting a worklist, ensure that the system has posted all results
from the worklist. If you delete a worklist while the system is posting results from
that worklist, the system may stop processing samples.
NOTE: You must have the appropriate level of security to access Data
Administration.
NOTE: Do not delete results or worklists if the system is in the Cleaning Failed
state. Ensure that the system is in the Ready or the Warming Up state and then
delete the results or worklists.
1. At the workspace, select Setup.
2. Select Data Administration.
3. At the Setup – Data Administration window, select Worklist.
CAUTION
Use care when deleting results. Deleting results permanently removes the results
from the database.
CAUTION
Use caution when deleting a worklist with unreleased results. When you delete a
worklist with unreleased results, the system permanently removes the associated
unreleased results from the database. If the unreleased results are from an open
bracket, the associated samples do not display in the Results Discarded report
generated at the Print – Report Options window or the Results Discarded view at
the Worklist – Summary window.
NOTE: When you delete a worklist from an open control-bracketed test, the
system does not close the bracket. The system discards any retained patient
results. However, it does not delete the controls.
4. Select Delete.
5. At the Setup – Data Administration Options window, you can delete the entire
worklist or part of the worklist:
• Select Delete Entire Worklist to request the deletion of the entire
worklist.
Continue with step 6.
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Printing Results
The system offers 3 printed report types for managing patient results:
• Results Report
• Test Report
• Runtime Report
When you select a report, the system displays the options for that report type.
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Printing Notes
When you turn off or disconnect the printer at your system, ensure that you
deselect the Automatic Runtime Results Report option at the Setup – Print
Options window and do not request any other printed report.
If the ADVIA Centaur XP system responds to an automatic or manual print
request when your printer is turned off or disconnected, a software error can
occur.
If you request a printed report when no printer is available, use the following
procedure to cancel the print request:
1. At the workspace, select Print.
2. Select Cancel Reports.
If you want to stop printing reports and you select Cancel Reports, printing does
not stop until everything in the printer buffer prints.
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ADVIA Centaur XP Operator’s Guide: Calibration
3 Calibration
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ADVIA Centaur XP Operator’s Guide: Calibration
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Calibration
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3-2 ADVIA Centaur XP Operator’s Guide: Calibration
The Master Curve for a sandwich assay has a positive slope, which indicates that
the analyte concentration is directly proportional to the light emission in RLUs.
When patient samples or quality control samples are assayed using a quantitative
assay, the system matches the adjusted RLU value to its corresponding analyte
concentration using the Master Curve.
Qualitative assays have a cut-off point that remains constant for each lot number
of reagent. The manufacturer establishes this cut-off during product development
and bases it on extensive population studies. Manufacturing assigns the analyte
concentration associated with the cut-off as, for example, an index value of 1.0.
See the method specific product insert for the method cut-off value.
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ADVIA Centaur XP Operator’s Guide: Calibration 3-3
The RLU relative to the cut-off is the basis for a Master Curve consisting of 6 to
10 standards. The cut-off or index value is represented as a specific level on the
Master Curve in Figure 3-3.
1 Frequency
2 Nonreactive
3 Reactive
4 Cutoff
5 Index
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3-4 ADVIA Centaur XP Operator’s Guide: Calibration
The reference serum factor is printed on the Calibrator Assigned Value Card
provided with Calibrator A.
To report results as % T Uptake, the system requires a % T Uptake reference
(normal) range. The system multiplies the T Uptake ratio by the midpoint of the %
T Uptake reference range to obtain % T Uptake results.
For example, if the % T Uptake reference range is defined as 25 to 35%
(midpoint of 30) and a sample has a T Uptake ratio of 0.82, the system calculates
the % T Uptake as follows:
0.82 × 30 = 24.6%
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ADVIA Centaur XP Operator’s Guide: Calibration 3-5
For competitive assays, this value is the difference between the mean RLU
value of the low calibrator and the mean RLU value of the high calibrator
divided by the mean RLU value of the high calibrator.
Ratio = (Low Cal Mean RLU - High Cal Mean)/High Cal Mean
• The low and high cal deviations are a measure of how well the high and the
low calibrator RLUs fit the Master Curve.
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3-6 ADVIA Centaur XP Operator’s Guide: Calibration
These values are a relative measure of the deviation of the average RLUs of
the low calibrator and the high calibrator from the Master Curve.
1 RLUs
2 High cal deviation
3 Master curve
4 Low cal deviation
5 Concentration
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ADVIA Centaur XP Operator’s Guide: Calibration 3-7
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3-8 ADVIA Centaur XP Operator’s Guide: Calibration
In Figure 3-5, the first 4 calibrations (1 – 4) of a reagent lot and calibrator lot
combination are valid. The system begins calculating observed ranges (5) after the
fourth valid calibration. Given this information, you can interpret the following
information correctly:
• The point labeled 6 is a valid calibration because it is within the observed
range.
• The point labeled 7 is an invalid calibration because it is outside of the
defined range.
• The point labeled 8 is an invalid calibration because it is outside of the
observed range.
Retained Calibration
To reduce unnecessary adjustments from calibration to calibration that can cause
minor calibration drift or trends, you can use the Retain Cal feature. The Retain
Cal feature retains a previously valid calibration to calculate patient and control
results. Select the Retain Cal option at the Test – Calibration window. Refer to
Defining Calibration Options‚ page 8-16.
For the first 4 calibrations of a reagent lot and calibrator lot combination, the
system gathers statistical data and uses these calibrations, in turn until expiration,
to calculate results. When you select the Retain Cal option, at the fifth and
subsequent calibrations the system can use data from a previous valid calibration
to calculate results if the new calibration is not statistically different from that
previous calibration.
When the system uses data from a previous calibration to calculate results, the
calibration interval expiration date is based on the date of the new calibration. The
system continues to use the same data to calculate results as long as calibrations
do not statistically differ from the retained calibration. When a new calibration is
not statistically different from a previous valid calibration, it meets the retained
calibration limits.
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ADVIA Centaur XP Operator’s Guide: Calibration 3-9
If a new calibration does not meet the retained calibration limits, the system uses
the new calibration to calculate results and set the calibration interval expiration
date. The system repeats the process until you use a new reagent lot or calibrator
lot. The process starts over with 4 new valid calibrations as in Figure 3-6.
In Figure 3-6, the first 4 calibrations (1-4) of a reagent lot and calibrator lot
combination are valid. The system calculates observed ranges after the fourth
valid calibration.
When the fifth calibration is a valid calibration that is not statistically different
from the fourth calibration, the system retains the fourth calibration and uses it to
calculate results. The system bases the calibration interval expiration date on the
date of this (fifth) calibration.
The sixth calibration is a valid calibration that is statistically different from the
fourth calibration. The system uses data from this calibration to calculate results
and set the calibration interval expiration date.
The seventh calibration is a valid calibration that is not statistically different from
the sixth calibration, so the system retains the sixth calibration and uses it to
calculate results. The system bases the calibration interval expiration date on the
date of this (seventh) calibration.
The system bases the calibration interval expiration date on the date of this
(seventh) calibration.
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ADVIA Centaur XP Operator’s Guide: Calibration 3-11
4. Scan the barcodes on the Master Curve Card from top to bottom.
5. Select Save.
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ADVIA Centaur XP Operator’s Guide: Calibration 3-13
Positions of Ratio and Slope on the Master Curve Card are reversed from
those positions on the user interface. When manually entering the
information, be sure to enter the information into the correct field.
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Procedural Notes
• Manual entry of information into the Stability Data field requires 12 digits.
Enter the 3-digit stability data on the Master Curve Card and follow it with 9
zeros (0).
• Positions of Ratio and Slope on the Master Curve Card are reversed from
those positions on the user interface. When manually entering the
information, ensure you enter the information into the correct field.
Use this procedure to delete a Master Curve before calibrations have been
performed against it. For example, if you typed incorrect information into 1 of the
fields and selected Save before you noticed the error, you can delete the Master
Curve and start over.
1. At the workspace, select Calibration.
2. Select Master Curve Definition.
3. At the Calibration – Master Curve Definition window, select the test and lot
number.
4. Select Delete.
This button is not available if a calibration has been performed for the Master
Curve.
5. Select Yes.
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ADVIA Centaur XP Operator’s Guide: Calibration 3-15
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3-16 ADVIA Centaur XP Operator’s Guide: Calibration
CAUTION
Use care when deleting a calibrator definition. Deleting a calibrator definition
permanently removes all associated calibration data and sample results from the
database.
4. Select Delete.
5. Select Yes.
Performing Calibrations
Use the procedures in this section to review the status of calibrations and to
schedule a calibration. Calibration intervals are assay specific. Refer to the
ADVIA Centaur Assay Manual.
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ADVIA Centaur XP Operator’s Guide: Calibration 3-17
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3-18 ADVIA Centaur XP Operator’s Guide: Calibration
Scheduling Calibrators
Calibrate an assay in the following instances:
• the calibration interval expires
• a calibration is invalid
• you use a new lot of assay reagents
• controls are repeatedly out of range
CAUTION
Do not schedule and load the calibrators before processing the end-of-bracket
controls of an open bracket for control-bracketed tests. If you schedule and load
calibrators for a control-bracketed test and the system aspirates a calibrator before
processing the end-of-bracket controls, the system discards any retained patient
test results.
For all tests, you must load the reagent lot you want to calibrate before you
schedule a calibrator.
1. At the workspace, select Worklist.
2. Select Schedule.
3. At the Worklist – Schedule window, select Calibrator.
4. Select how you want the system to identify the calibrator:
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ADVIA Centaur XP Operator’s Guide: Calibration 3-19
• If the sample identifier selected at the Setup – Tube Type & Barcode
window is SID, you can select Schedule by SID or Schedule by Rack.
• If the sample identifier selected at the Setup – Tube Type & Barcode
window is Rack, you can only select Schedule by Rack.
Refer to Specifying Sample Identification‚ page 8-4.
5. Select or enter a test for the calibrator.
If you use the keyboard to enter the test in the Test field, you must enter the
test name exactly as it is displayed on the schedule window.
The system automatically displays the calibrators previously defined for the
test. You define the calibrator SIDs at the Calibration – Calibrator Definition
window.
Calibrators do not display in the following situations:
• calibrators are not defined
• Master Curves are not defined
• no reagent is onboard
• the calibration material for defined lots is expired
6. Select a calibrator.
• When scheduling by SID, select the appropriate SID. The system fills in
the Rack ID.
• When scheduling by Rack, you can select specific racks for the
calibrators.
7. You can select a reagent lot.
8. If you schedule by rack, enter the Rack ID in Rack.
The Rack ID consists of 4 numbers (0001–9999) followed by a letter (A–E).
Enter the letter in uppercase.
9. Select Save.
10. Repeat steps 4 through 9 to schedule additional tests for a calibrator.
11. Load low and high calibrators in a rack.
12. Load the rack in the sample entry queue.
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ADVIA Centaur XP Operator’s Guide: Calibration 3-21
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3-22 ADVIA Centaur XP Operator’s Guide: Calibration
Flag Description
Failed Defined Ratio The calibration is outside the range for the ratio that is defined
at the Calibration – Master Curve Definition window. Schedule
another calibration at the Worklist – Schedule window.
Failed Defined The calibration is outside the range for the slope that is defined
Slope at the Calibration – Master Curve Definition window. Schedule
another calibration at the Worklist – Schedule window.
Failed Observed The calibration exceeded the high calibrator deviation that is
High Cal Deviation calculated by the system. Schedule another calibration at the
Worklist – Schedule window.
Failed Observed The calibration exceeded the low calibrator deviation that is
Low Cal Deviation calculated by the system. Schedule another calibration at the
Worklist – Schedule window.
Failed Observed The calibration is outside the range for the ratio that is
Ratio calculated by the system. Schedule another calibration at
the Worklist – Schedule window.
Failed Observed The calibration is outside the range for the slope that is
Slope calculated by the system. Schedule another calibration at
the Worklist – Schedule window.
Failed % Neut The neutralizing reagent did not reduce the assay signal for the
high calibrator to the defined level. Schedule another calibration
at the Worklist – Schedule window.
Failed Rgt A The calibration did not yield the expected results for the
Stability Reagent A stability check. Schedule another calibration at
the Worklist – Schedule window.
Failed Rgt B Ratio The calibration did not yield the expected results for the
Reagent B ratio check. Schedule another calibration at the
Worklist – Schedule window.
High CV The CV (coefficient of variation) for the replicates of the
calibrator is above the acceptable CV entered in the Master
Curve definition. You can manually exclude a replicate at the
Calibration – Data window if you have processed more than the
minimum number of replicates and Autoexclude Cal Replicates
is not selected at the Test – Calibration window. If you cannot
manually exclude a replicate, schedule another calibration at
the Worklist – Schedule window.
Rgt A The CV (coefficient of variation) for the high calibrator
High Cal CV replicate RLU values for Reagent A was too large. Schedule
another calibration at the Worklist – Schedule window.
Rgt A The CV (coefficient of variation) for the low calibrator replicate
Low Cal CV RLU values for Reagent A was too large. Schedule another
calibration at the Worklist – Schedule window.
Rgt B The CV (coefficient of variation) for the high calibrator
High Cal CV replicate RLU values for Reagent B was too large. Schedule
another calibration at the Worklist – Schedule window.
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ADVIA Centaur XP Operator’s Guide: Calibration 3-23
Flag Description
Rgt B The CV (coefficient of variation) for the low calibrator replicate
Low Cal CV RLU values for Reagent B was too large. Schedule another
calibration at the Worklist – Schedule window.
System Excluded The system automatically excluded a calibrator replicate RLU.
This flag only displays if Autoexclude Cal Replicates is selected
at the Test – Calibration window.
Too Few Replicates The number of acceptable replicate results is less than the
number required to calibrate the test. The minimum number of
acceptable replicates is defined at the Test – Calibration
window. Schedule another calibration at the Worklist –
Schedule window.
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3-24 ADVIA Centaur XP Operator’s Guide: Calibration
10. Close and reopen the Calibration – Data window to display the symbol
indicating that the calibrator RLU is excluded.
11. If required, you can select Include and then select Save to include an
excluded calibrator RLU in the calibration.
Waiving a Calibration
CAUTION
Do not waive calibrations unnecessarily. Waiving Cals repeatedly may cause
misreported results.
When the calibration interval for a test expires while samples are in process, you
can finish processing samples scheduled for the test by waiving the calibration.
Use the Waive Cal option only when tests are Inprocess.
The system calculates results for the samples and displays the results for the test
as Waived. You must have the appropriate level of security to waive a calibration
and you must select Waive Cal at the Test – Calibration window.
When you waive a calibration, enter a comment and recalibrate immediately after
this. Waiving calibrations repeatedly may cause misreported results. A waived
calibration remains waived until the next calibration interval.
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ADVIA Centaur XP Operator’s Guide: Calibration 3-25
Assigning a Calibration
If a calibration is invalid, you can assign a valid calibration to samples that the
system processed with that invalid calibration. You can assign the last valid
calibration or recalibrate and assign the next valid calibration. The system
calculates results for the samples and displays the results for the test as Cal
Assigned.
You must have the appropriate level of security to assign a calibration to results
and you must enable Assign Cal at the Test – Calibration window.
1. At the workspace, select Worklist.
2. Select Summary.
3. At the Worklist – Summary window, select a final result with RLU instead of
a concentration value in the result column.
4. Select Assign Cal.
5. At the Worklist – Assign Calibration window, select the results to which you
want to assign a calibration.
6. The system displays the sample SID for the result you selected at the Worklist
– Summary window.
7. Select Continue.
8. At the Worklist – Summary window, select Save.
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3-26 ADVIA Centaur XP Operator’s Guide: Calibration
No Current Calibration
If all of the following conditions apply:
• You have no current valid calibration for the reagent lot that you are using
because it is a new lot.
• You process patient and control samples with calibrators.
• You perform a calibration and the calibration is invalid because it exceeds
the defined ranges.
The system performs the following actions:
• Stops aspirating samples for that test.
• Continues with inprocess patient and control samples and displays RLUs
for results if Assign Cal is selected in the test definition.
You can perform the following actions to report patient results:
• You must repeat the calibration and any patient and control samples that
were not processed.
The repeated calibration must be valid.
• You can use the Assign Cal feature to assign the new valid calibration to
the patient and control samples with RLU results from the invalid calibration.
Control values that are within range indicate that the RLUs of patient samples
processed with the invalid calibration are accurate.
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ADVIA Centaur XP Operator’s Guide: Calibration 3-27
Control values that are within range indicate that the RLUs of patient samples
processed with the invalid calibration are accurate.
• You must repeat any samples that were not aspirated with a rescheduled
calibration.
The repeated calibration must be valid. The system does not process samples
with requests for tests with an invalid calibration.
Expired Calibration
If all of the following conditions apply:
• You have an expired calibration.
• You process patient and control samples with calibrators.
• You perform a calibration and the calibration is invalid because it exceeds
the defined ranges.
The system performs the following actions:
• Stops aspirating samples for that test.
• Continues with inprocess patient and control samples and displays RLUs
for results if Assign Cal is selected in the test definition.
You can perform the following actions to report patient results:
• You can waive the expired calibration and use the Assign Cal feature to assign
the waived calibration to the patient and control samples with RLU results
from the invalid calibration.
Control values that are within range indicate that the RLUs of patient samples
processed with the invalid calibration are accurate.
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ADVIA Centaur XP Operator’s Guide: Quality Control
4 Quality Control
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Using Quality Control Features
The ADVIA Centaur XP system allows you to define each control for use on the
system. The system has the following data storage capabilities:
• allows you to define up to 50 tests per control
For example, for Ligand Plus 1 you can define up to 50 tests including TSH-3,
TSH, T3, T4, FT3, FrT4, and TUp.
• allows you to define up to 300 controls
• ability to store results for up to 10 lots of data for each test/control
combination
For example, for the ADVIA Centaur XP PSA assay you can define 10
different lot numbers of Ligand Plus level 1, Ligand Plus level 2, and Ligand
Plus level 3. This helps you manage transitions between lots of quality control
material.
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4-2 ADVIA Centaur XP Operator’s Guide: Using Quality Control Features
• Routine controls are not provided with the assay reagent kits.
Control such as Ligand Plus or Tumor Marker Plus are routine controls.
• Kit control have specific assay reagent kits.
NOTE: If you are defining a control that does not have a leading K in the
barcode, select SID and edit that field to match the barcode of the control. You
can select SID only after entering the lot number.
6. Enter the information for the new lot of control, such as type, lot, and
expiration date.
The lot number and the expiration date are located on the quality control
material packaging. You must enter a lot number and an expiration date to
save the control definition.
You can enter up to 7 characters for the lot number.
Enter the date in the format DD MMM YY, for example 26 Oct 99.
The system fills in the SID field with the control lot number preceded by K.
The SID can be up to 13 characters. If the control is a Routine type and your
QC SIDs are different than the lot number, you can edit the SID.
7. Select a Test:
a. Select the Test button.
The Test–Selection window displays.
b. Select a test.
c. To close the Test–Selection window, select Continue.
8. Enter the expected values.
9. Select Save Test.
10. Repeat steps 3 through 9 for all tests for the control.
11. Select Save.
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ADVIA Centaur XP Operator’s Guide: Using Quality Control Features 4-3
Deleting a Control
1. At the workspace, select Quality Control.
2. Select Control Definition.
3. Select the name of the control.
4. Select the information for the lot of control, such as lot and type.
CAUTION
Use care when deleting a test from a control definition. Deleting a test from a
control definition permanently removes all associated stored data and results from
the database.
5. Select Delete.
6. Select Yes.
7. To delete a test from a control definition, select the test, and then select Delete
Test.
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4-4 ADVIA Centaur XP Operator’s Guide: Using Quality Control Features
Pool Number
You can enter up to 4 numbers for the pool number. For customers using the QC
Online service, the pool number is assigned by the service. If you use your own
quality control pools, you can assign a pool number to track results.
Editing a QC Definition
Use this procedure to edit a QC definition:
1. At the workspace, select Quality Control.
2. Select Control Definition.
3. At the Quality Control – Control Definition window, select a control name
and a lot number.
4. Select Edit.
5. Edit the QC definition.
CAUTION
Use care when deleting a test from a control definition. Deleting a test from a
control definition permanently removes all associated stored data and results from
the database.
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ADVIA Centaur XP Operator’s Guide: Using Quality Control Features 4-5
Scheduling QC Samples
Use this procedure to schedule controls. As a minimum requirement, you must
assay 2 levels of quality control material on each day that samples are analyzed.
You must also assay quality control samples when performing a 2-point
calibration. Refer to the ADVIA Centaur Assay Manual for assay-specific QC
recommendations.
1. At the workspace, select Worklist.
2. Select Schedule.
3. At the Worklist – Schedule window, select Control.
NOTE: If you are scheduling controls for a control-bracketed test, you must
schedule by SID. The system does not allow you to schedule samples for
control-bracketed tests by Rack.
4. Select how you want the system to identify the control:
• If the sample identifier selected at the Setup – Tube Type & Barcode
window is SID, you can select Schedule by SID or Schedule by Rack.
The control SIDs are defined at the Quality Control – Control Definition
window.
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4-6 ADVIA Centaur XP Operator’s Guide: Using Quality Control Features
• If the sample identifier selected at the Setup – Tube Type & Barcode
window is Rack, you can only select Schedule by Rack.
Refer to Specifying Sample Identification‚ page 8-4.
5. Select or enter the tests or the profiles for the control.
If you use the keyboard to enter the tests or profiles in the Test field or the
Profile field, you must enter the test name or profile exactly as it displays on
the schedule window.
The controls previously defined for the tests are automatically displayed.
6. Select the controls.
7. If you schedule by rack, enter the Rack ID in Rack.
The Rack ID consists of 4 numbers (0001–9999) followed by a letter (A–E).
Enter the letter in uppercase.
8. Select Save.
9. Repeat steps 4. through 8. to schedule tests or profiles for additional controls.
10. Load sample cups of QC material in a rack.
11. Load the rack in the sample entry queue.
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ADVIA Centaur XP Operator’s Guide: Using Quality Control Features 4-7
Ensure that the start-of-bracket control set and the end-of-bracket control set
are in different racks. Ensure that all of the controls within a set are in the
same rack.
• If you schedule the start-of-bracket controls and wait until they are processed
and have acceptable results before scheduling the end-of-bracket controls,
schedule the controls by SID.
You do not need to enter the rack ID and position of the controls. You can use
the same rack for the start-of-bracket controls and end-of-bracket controls as
long as the system processed the previous set of controls with acceptable
results and ejected the rack.
• You must schedule and load the end-of-bracket controls within the acceptable
time interval displayed in the Control Warning field at the Test – Definition
window.
Failure to do so causes the system to discard retained patient results. Refer to
Viewing Control Bracketing Features‚ page 8-17.
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4-8 ADVIA Centaur XP Operator’s Guide: Using Quality Control Features
Ensure that the rack ID for the end-of-bracket controls is different from the rack
ID of the start-of-bracket controls.
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ADVIA Centaur XP Operator’s Guide: Performing Maintenance
5 Performing Maintenance
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ADVIA Centaur XP Operator’s Guide: Performing Maintenance
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Performing Maintenance
The ADVIA Centaur XP system monitors maintenance activities and notifies you
when a scheduled activity is due. In addition, the system notifies you through a
color change at the Maintenance Status button and the Status – Maintenance
window when routine and extended certification procedures are due. When a
certification is due, contact your local technical support provider to schedule the
procedure as soon as possible.
The system provides a maintenance schedule for you to record the completion of
scheduled maintenance activities. The system then uses this information to
automatically update the maintenance schedule with the next time the activity is
due.
Reviewing Maintenance
At the Status – Maintenance window, the background of the maintenance
activities changes color to indicate status:
• Yellow indicates that a maintenance activity is due or overdue.
• Red indicates that an automated maintenance procedure did not finish.
At the workspace, the background of the Maintenance Status button also changes
color to indicate status. You can select the Maintenance Status button to open the
Status – Maintenance window and display information about maintenance
activities.
Select the appropriate View and Sort combinations to display specific information
at the Status – Maintenance window:
• The View that you select determines the information that the system displays.
For example, if you select Schedule, the system displays the scheduled
maintenance activities.
• The Sort that you select determines the order in which the system displays the
scheduled activities.
For example, if you select Activity, the system sorts and displays the
scheduled activities in alphabetical order.
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5-2 ADVIA Centaur XP Operator’s Guide: Performing Maintenance
The following table describes the View and Sort combinations you can use to
perform some routine tasks:
Task View Sort
Determine which maintenance activities are due Schedule Time Due
Find a specific maintenance activity Schedule Activity
Review the maintenance activities that were performed Log Time Completed
most recently
Review the history of a specific maintenance activity Log Activity
Recording Maintenance
After performing a scheduled maintenance activity you can record that activity
as complete. You can also enter and review comments.
NOTE: If you are not currently signed in, you must sign in to the system.
1. At the workspace, select Maintenance Status.
2. At the Status – Maintenance window, select an activity.
3. Select Completed.
The system automatically enters your initials and calculates the date when the
activity is due next.
4. You can enter Comments, such as a note that indicates if the maintenance
was performed to correct a system problem:
a. Select Log in View.
b. Select Time Completed in Sort.
c. Select an activity.
d. Select Comments.
e. At the Comments window, enter a comment.
You can enter up to 3 comments. Comments can be up to 120
alphanumeric characters. The system enters your initials and the date.
f. Select Save.
Repeat these steps to update the maintenance schedule and enter comments for
another activity.
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ADVIA Centaur XP Operator’s Guide: Performing Maintenance 5-3
3. Select Procedure.
An online help window opens and displays the maintenance procedure.
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5-4 ADVIA Centaur XP Operator’s Guide: Performing Maintenance
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ADVIA Centaur XP Operator’s Guide: Performing Maintenance 5-5
WARNING
Do not handle cleaning solution without wearing protective equipment, including
gloves, laboratory coat, and safety glasses or protective face shield.
CAUTION
Do not neglect to perform daily cleaning. Choosing to not perform the
cleaning procedure can result in bacterial contamination, paramagnetic
particle buildup, protein buildup, and crystallization, which can affect patient
results.
NOTE: You cannot process samples while the cleaning procedure is in progress.
1. If the bottle is more than half full, empty the waste bottle.
2. If necessary, replenish the cleaning solution.
Refer to Preparing the Cleaning Solution‚ page 5-17.
3. Ensure that the water bottle is at least 1-quarter full (approximately 2.5 L).
NOTE: If the diluter drawer is not closed (up), the daily cleaning procedure
does not prime the wash station diluters correctly.
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1 Diluter drawer
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ADVIA Centaur XP Operator’s Guide: Performing Maintenance 5-7
CAUTION
Do not grasp the tubing when disconnecting the tubing from the top of the
water bottle or reservoir. Pulling the tubing can cause crimps. Grasp the
fittings, not the tubing.
c. Disconnect the water tubing from the water bottle and set aside on
lint-free tissues or gauze to absorb drips.
d. Lift the water bottle up and out.
2. Remove the cap.
3. Fill the spare, clean water bottle with fresh reagent water and install the cap.
Refer to Reagent Water Quality‚ page C-4.
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5-8 ADVIA Centaur XP Operator’s Guide: Performing Maintenance
WARNING
Do not clean the primary reagent compartment while the probes are accessing
reagent packs. Injury can result.
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ADVIA Centaur XP Operator’s Guide: Performing Maintenance 5-9
CAUTION
Use care when handling the glass sensors. Glass sensors are fragile and errors
occur on the system when the sensors are damaged.
a. Move the clamps that cover the sensors aside, carefully remove the
sensors, and let them hang in the drawer.
CAUTION
Do not grasp the tubing when disconnecting the tubing from the top of the
water bottle or reservoir. Pulling the tubing can cause crimps. Grasp the
fittings, not the tubing.
b. Disconnect the water tubing fittings and set them aside on paper towels.
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5-10 ADVIA Centaur XP Operator’s Guide: Performing Maintenance
1 Reservoir cap
2 Sensor
3 Water tubing fittings
4 Sensor
5 Manifold
6 Water reservoir
7 Water bottle
6. Remove the reservoir cap and empty any water from the reservoir.
7. Remove the manifold and install the water reservoir cleaning cover:
a. Loosen the 3 knurled captive screws.
b. Remove the manifold from the reservoir.
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ADVIA Centaur XP Operator’s Guide: Performing Maintenance 5-11
CAUTION
Do not leave cleaning solution residue on the sensors. Residual cleaning solution
on the sensors can affect assay results. Clean and rinse the sensors thoroughly.
b. Clean the sensors by wiping the sensors with lint-free tissues or gauze
saturated with cleaning solution.
c. Rinse the sensors by wiping the sensors with lint-free tissues or gauze
saturated with reagent water.
d. Repeat step c. with fresh lint-free tissues or gauze.
e. Install the sensors in the top of the reservoir.
f. Secure the sensors with the clamps.
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ADVIA Centaur XP Operator’s Guide: Performing Maintenance 5-13
CAUTION
Do not leave cleaning solution residue in the water reservoir. Residual cleaning
solution in the water reservoir tubing can affect assay performance. Rinse the
water reservoir thoroughly with fresh reagent water.
h. Thoroughly rinse the inside of the reservoir, and the reservoir cleaning
cover at least 3 times to ensure that there is no residual cleaning solution
on the components.
i. Fill the reservoir with reagent water.
j. Install the cleaning cover on the reservoir.
k. Invert the reservoir, swirl the water a few times, and return the reservoir
to the upright position.
l. Remove the cleaning cover and empty the water from the reservoir.
m. Invert the reservoir to dry.
15. Clean and rinse the water bottle:
a. Remove the cap and empty any water from the water bottle that was
removed in step 10..
b. Pour approximately 1 L of cleaning solution into the water bottle.
c. Place the cap on the bottle and close tightly.
d. Swirl the solution around the inside of the bottle a few times.
e. Press some gauze over the holes in the top of the bottle and hold the gauze
with your fingers.
f. Invert the bottle, swirl the solution a few times and then return the bottle
to the upright position.
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CAUTION
Do not leave cleaning solution residue in the water bottle. Residual cleaning
solution in the water bottle tubing can affect water filter and assay
performance. Rinse the water bottle thoroughly with fresh reagent water.
j. Rinse the inside of the bottle including the tubing at least 5 times with
fresh reagent water.
k. Invert the bottle to dry.
1 Manifold tubing
2 Manifold
CAUTION
Do not touch the tubing or sensors. Touching the tubing or sensors can cause
contamination.
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ADVIA Centaur XP Operator’s Guide: Performing Maintenance 5-15
e. Rinse each tubing by filling it with reagent water using a clean transfer
pipette, removing the fluid and repeating 5 times.
CAUTION
Do not leave cleaning solution residue on the manifold. Residual cleaning
solution on the manifold can cause corrosion. Rinse the manifold thoroughly
with fresh reagent water, including caps, captive screws, and clamps.
f. Thoroughly rinse the manifold and remove excess water from the
manifold and tubing.
CAUTION
Do not remove the water trap when the system is in the Inprocess state or the
Cleaning state. Removing the water trap while the system is in one of these
states can cause a low vacuum error and cause the system to stop sampling and
cancel all of the tests in the ring.
1. Ensure that the system is in 1 of the following states:
• Ready
• Warming Up
• Diagnostic
• Mechanics Off
• Check Status
BIOHAZARD
Wear personal protective equipment. Use universal precautions. Refer to Safety
Instructions‚ page A-1 for recommended precautions when working with
biohazardous materials.
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1 Water trap
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ADVIA Centaur XP Operator’s Guide: Performing Maintenance 5-17
You need the following materials to perform the Monthly Cleaning Procedure:
• approximately 4 L of prepared ADVIA Centaur XP Cleaning Solution for the
automated portion of the Monthly Cleaning Procedure
• approximately 2 L of prepared ADVIA Centaur XP Cleaning Solution used
for soaking and wiping system components
• reagent water
• large maintenance brush
• lint-free tissues, gauze, and paper towels
• spare air filter
WARNING
Do not handle cleaning solution without wearing protective equipment, including
gloves, laboratory coat, and safety glasses or protective face shield.
Use this procedure to prepare the cleaning solution for soaking and wiping system
components.
1. Carefully pour 1 container of ADVIA Centaur XP Cleaning Solution
Concentrate into the container used for soaking system components.
You can soak the components in a sink, the cuvette waste bin liner, or another
suitable container.
2. Add 2 L of water to the container.
WARNING
Do not handle cleaning solution without wearing protective equipment, including
gloves, laboratory coat, and safety glasses or protective face shield.
CAUTION
Do not bend the probe. Bending the probe can cause system errors.
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3. Loosen the screw on the left side of the system that secures the top cover and
then open the top cover.
WARNING
Use care when working with the reagent probes and ancillary probe. The probe
tips are sharp and can cause skin punctures.
4. Remove the reagent probes cover by lifting the cover up and then out.
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ADVIA Centaur XP Operator’s Guide: Performing Maintenance 5-19
1 Reagent probe 1
2 Reagent probe 2
3 Reagent probe 3
4 Probe – side view.
5 Motor.
b. Use a gentle downward motion to wipe each probe with lint-free tissues
or gauze saturated with cleaning solution.
c. Check the probes for damage and to ensure that they are not bent.
CAUTION
Do not leave cleaning solution residue on the probe. Residual cleaning solution
on the probe can damage the probe. Follow the instructions for rinsing the
probe.
d. Thoroughly rinse the reagent probe, especially the middle of the probe.
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Rinse the probe using a gentle downward motion to wipe each probe with
lint-free tissues or gauze saturated with reagent water.
1 Reagent probe
WARNING
Do not handle cleaning solution without wearing protective equipment, including
gloves, laboratory coat, and safety glasses or protective face shield.
1. At the workspace, select System Status.
2. Select Turn System Mechanics Off.
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3. Loosen the screw on the left side of the system that secures the top cover and
then open the top cover.
WARNING
Use care when working with the reagent probes and ancillary probe. The probe
tips are sharp and can cause skin punctures.
CAUTION
Do not bend the probe. Bending the probe can cause system errors.
b. Pull the probe toward you by holding the top of the probe assembly.
c. Check the probe for damage and to ensure that it is not bent.
d. Use a gentle downward motion to wipe the probe with lint-free tissues or
gauze saturated with cleaning solution.
e. Thoroughly rinse the ancillary probe, especially the middle of the probe.
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Rinse the probe using a gentle downward motion to wipe each probe with
lint-free tissues or gauze saturated with reagent water.
1 Probe assembly
2 Ancillary probe
WARNING
Do not handle cleaning solution without wearing protective equipment, including
gloves, laboratory coat, and safety glasses or protective face shield.
1. At the workspace, select System Status.
2. Select Turn System Mechanics Off.
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ADVIA Centaur XP Operator’s Guide: Performing Maintenance 5-23
3. Loosen the screw on the left side of the system that secures the top cover and
then open the top cover.
4. Carefully lift the probe arms up to the highest position.
NOTE: It is not necessary to remove the aspirate probes. Clean only the
visible portion of the probe.
5. Wipe the visible portion of the probes with lint-free tissues or gauze saturated
with cleaning solution.
Refer to Preparing the Cleaning Solution‚ page 5-17.
6. Wipe the probes with lint-free tissues or gauze saturated with reagent water.
1 Probe clip
2 Aspirate probe
CAUTION
Use care that you do not damage probes. To prevent damage to the probe, ensure
that each clip is in its locked position.
7. Move the aspirate probes down approximately 1.27 cm (0.5 in) from the
highest position.
8. Close and secure the top cover.
9. Select Turn System Mechanics On.
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CAUTION
Do not install a wet or damp air filter. The moisture can cause system damage.
CAUTION
Do not wash the air filter with cleaning solution. The cleaning solution can
damage the filter.
5. Rinse the air filter with water and allow it to dry thoroughly.
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ADVIA Centaur XP Operator’s Guide: Performing Maintenance 5-25
WARNING
Do not handle cleaning solution without wearing protective equipment, including
gloves, laboratory coat, and safety glasses or protective face shield.
CAUTION
Do not put cleaning solution in the water bottle until after you select Perform
Monthly Cleaning. If cleaning solution is in the water bottle too soon, it is pumped
throughout the system. Follow all instructions as written.
1. At the workspace, select Maintenance Status.
2. At the Status – Maintenance window, select Schedule in View.
3. Select Perform Monthly Cleaning.
4. Select Perform.
5. Select Yes.
The system empties the waste reservoir.
First Prompt
1. Fill the water bottle with 4 L of cleaning solution:
a. When prompted, pull out the waste and water drawer.
b. Remove the water bottle.
c. Carefully pour 2 containers of ADVIA Centaur XP Cleaning Solution
Concentrate into the empty water bottle.
d. Add 4 L of water to the water bottle.
2. Load the water bottle on to the system.
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CAUTION
Use care when handling the glass sensors. Glass sensors are fragile and errors
occur on the system when the sensors are damaged.
a. Disconnect the tubing and remove the sensors and cap from the water
reservoir.
b. Remove the water reservoir.
CAUTION
Do not leave water in the water reservoir during the cleaning procedure. Water in
the reservoir dilutes the cleaning solution. Empty the water reservoir completely.
c. Empty the water reservoir.
d. If the system uses an onboard waste bottle, remove the waste bottle and
empty the contents into a container approved for biohazardous waste.
4. Ensure that the water trap is emptied and the bottle lid is tight.
5. Load the empty water reservoir and waste bottle onto the system:
a. Install the empty water reservoir on the system.
b. Connect the tubing and install the sensors.
c. Ensure that the cap on the waste reservoir is tight.
d. Install the waste bottle on the system.
CAUTION
Do not select Cancel during the First Prompt. If you select Cancel, you must
remove the cleaning solution from the system and install the reagent water in a
clean water bottle before you select Continue.
6. Select Continue.
Second Prompt
1. Rinse the water bottle.
Remove and thoroughly rinse the water bottle and cap with reagent water.
2. Add 2 L of reagent water to the water bottle.
Do not add more than 2 L of water to the water bottle. Excess water causes the
waste bottle to fill before the end of the cleaning procedure, causing the
procedure to fail.
3. Place the water bottle on the system.
4. Ensure that the water reservoir contains no more than 1.27 cm (0.5 in) fluid.
5. Select Continue.
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ADVIA Centaur XP Operator’s Guide: Performing Maintenance 5-27
Third Prompt
1. Rinse the water bottle and the water reservoir:
a. Disconnect the tubing and remove the sensors from the water reservoir.
b. Remove the water bottle and the water reservoir.
c. Thoroughly rinse the water bottle and the water reservoir with reagent
water to remove residual cleaning solution.
2. Fill the water bottle with 2 L of water and load it onto the system.
3. Load the empty water reservoir onto the system.
Ensure that you connect the tubing and install the sensors.
4. Select Continue.
Fourth Prompt
1. Remove the water bottle and the water reservoir:
a. Disconnect the tubing and remove the sensors from the water reservoir.
b. Remove the water bottle and the water reservoir and set them aside.
2. Fill the alternate water bottle with reagent water and install it on the system:
a. Install the empty alternate water reservoir on the system.
b. Ensure that you connect the tubing and install the sensors.
c. Close the waste and water drawer.
3. Select Continue.
4. Observe the water filter.
5. If required, vent air through the air vent filter.
When priming is complete, the system returns to the Ready state.
6. Thoroughly rinse the water bottle and the water reservoir that you removed
from the system with reagent water, and then invert them to dry.
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5-28 ADVIA Centaur XP Operator’s Guide: Performing Maintenance
WARNING
Do not handle cleaning solution without wearing protective equipment, including
gloves, laboratory coat, and safety glasses or protective face shield.
CAUTION
Do not put cleaning solution in the water bottle until after you select Perform
Monthly Cleaning. If cleaning solution is in the water bottle too soon, it is pumped
throughout the system. Follow all instructions as written.
First Prompt
1. Fill the water bottle with 4 L of cleaning solution:
a. When prompted, pull out the waste and water drawer and remove the
water bottle.
b. Carefully pour 2 containers of ADVIA Centaur XP Cleaning Solution
Concentrate into the empty water bottle.
c. Add 4 L of water to the water bottle.
2. Load the water bottle onto the system.
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ADVIA Centaur XP Operator’s Guide: Performing Maintenance 5-29
CAUTION
Use care when handling the glass sensors. Glass sensors are fragile and errors
occur on the system when the sensors are damaged.
a. Disconnect the tubing and remove the sensors and float switch from the
water reservoir.
CAUTION
Do not leave water in the water reservoir during the cleaning procedure. Water in
the reservoir dilutes the cleaning solution. Empty the water reservoir completely.
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CAUTION
Do not select Cancel during the First Prompt. If you select Cancel, you must
remove the cleaning solution from the system and install the reagent water in a
clean water bottle before you select Continue.
7. Select Continue.
Second Prompt
1. Rinse the water bottle.
Remove and thoroughly rinse the water bottle and cap with reagent water.
2. Add 2 L of reagent water to the water bottle.
Do not add more than 2 L of water to the water bottle. Excess water causes the
waste bottle to fill before the end of the cleaning procedure, causing the
procedure to fail.
3. Place the water bottle on the system.
4. Ensure that the water reservoir contains no more than 1.27 cm (0.5 in) fluid.
5. Select Continue.
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ADVIA Centaur XP Operator’s Guide: Performing Maintenance 5-31
Third Prompt
1. Rinse the water bottle and the water reservoir:
a. Disconnect the tubing and remove the sensors from the water reservoir.
b. Remove the water bottle and the water reservoir.
c. Thoroughly rinse the water bottle and the water reservoir with reagent
water to remove residual cleaning solution.
2. Fill the water bottle with 2 L of reagent water and load it onto the system.
3. Load the empty water reservoir onto the system.
Ensure that you connect the tubing and install the sensors.
4. Select Continue.
Fourth Prompt
1. Remove the water bottle and the water reservoir:
a. Disconnect the tubing and remove the sensors and float switch from the
water reservoir.
b. Remove the water bottle and the water reservoir and set them aside.
2. Install the empty alternate water reservoir and the empty alternate water bottle
on the system.
Connect the tubing to the water reservoir and the water bottle.
3. Clean the float switch and sensors.
Clean the water reservoir float switch and the sensors using gauze soaked
with cleaning solution.
4. Rinse the water reservoir float switch and the sensors twice:
a. Rinse the water reservoir float switch and the sensors in a container of
reagent water.
b. Using fresh reagent water, rinse the water reservoir float switch and the
sensors again.
CAUTION
Do not touch the tubing or sensors. Touching the tubing or sensors can cause
contamination.
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As-Needed Maintenance
For online information about performing maintenance procedures, perform the
following steps:
1. At the workspace, select the Status Maintenance button.
2. At the Status – Maintenance window, select the appropriate maintenance task:
3. Select Procedure.
4. Follow the instructions in the online help.
NOTE: Most of these procedures are also available in this section of the
ADVIA Centaur XP Operator’s Guide.
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1 Screw
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5-34 ADVIA Centaur XP Operator’s Guide: Performing Maintenance
BIOHAZARD
Wear personal protective equipment. Use universal precautions. Refer to Safety
Instructions‚ page A-1 for recommended precautions when working with
biohazardous materials.
WARNING
Do not handle cleaning solution without wearing protective equipment, including
gloves, laboratory coat, and safety glasses or protective face shield.
WARNING
Use care when working with the reagent probes and ancillary probe. The probe
tips are sharp and can cause skin punctures.
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ADVIA Centaur XP Operator’s Guide: Performing Maintenance 5-35
CAUTION
Do not bend the probe. Bending the probe can cause system errors.
5. Push the reagent probe assemblies toward the incubation ring by pushing the
motor.
• Push reagent probe 1 to the right and then toward the incubation ring.
• Push reagent probe 2 to the left and then toward the incubation ring.
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1 Reagent probe 1
2 Reagent probe 2
3 Reagent probe 3
4 Probe
5 Motor
6. Gently push the sample probe and ancillary probe assemblies back toward the
incubation ring by pushing the motors.
7. Use a disposable transfer pipette to add 0.5 to 1.0 mL of cleaning solution to
each rinse station.
Refer to Preparing the Cleaning Solution‚ page 5-17.
8. Clean each rinse station using the small maintenance brush.
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ADVIA Centaur XP Operator’s Guide: Performing Maintenance 5-37
9. Gently insert the brush into the rinse station to a depth of 6.3 cm (2.5 in), and
swirl the brush inside the rinse station.
1 Small brush
2 Rinse station
10. Remove the brush and then remove the cleaning solution with a transfer
pipette.
11. Clean each rinse station:
a. Wipe the rinse station with lint-free tissues or gauze saturated with
cleaning solution.
b. Wipe the rinse station with lint-free tissues or gauze saturated with
reagent water.
12. Close and secure the top cover.
13. Select Turn System Mechanics On.
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5-38 ADVIA Centaur XP Operator’s Guide: Performing Maintenance
BIOHAZARD
Wear personal protective equipment. Use universal precautions. Refer to Safety
Instructions‚ page A-1 for recommended precautions when working with
biohazardous materials.
WARNING
Do not handle cleaning solution without wearing protective equipment, including
gloves, laboratory coat, and safety glasses or protective face shield.
1. At the workspace, select System Status.
2. Select Turn System Mechanics Off.
3. Loosen the screw on the left side of the system that secures the top cover and
then open the top cover.
4. Remove the primary reagent packs from the primary reagent compartment
and refrigerate them.
5. Remove the reagent probes cover by lifting the cover up and then out.
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ADVIA Centaur XP Operator’s Guide: Performing Maintenance 5-39
6. Push the reagent probe assemblies toward the incubation ring by pushing the
motors.
• Push reagent probe 1 to the right and then toward the incubation ring.
• Push reagent probe 2 to the left and then toward the incubation ring.
• Push reagent probe 3 toward the incubation ring.
1 Reagent probe 1
2 Reagent probe 2
3 Reagent probe 3
4 Probe
5 Motor
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5-40 ADVIA Centaur XP Operator’s Guide: Performing Maintenance
1 Shutter drive
2 Shutter drive arm
3 Reagent probe shutter
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ADVIA Centaur XP Operator’s Guide: Performing Maintenance 5-41
BIOHAZARD
Wear personal protective equipment. Use universal precautions. Refer to Safety
Instructions‚ page A-1 for recommended precautions when working with
biohazardous materials.
WARNING
Do not clean the primary reagent compartment while the probes are accessing
reagent packs. Injury can result.
1. At the workspace, select System Status.
2. Select Turn System Mechanics Off.
3. Open the primary reagent door.
4. Remove the primary reagent packs from the primary reagent compartment
and refrigerate them.
5. Wipe excess water from the primary reagent compartment.
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5-42 ADVIA Centaur XP Operator’s Guide: Performing Maintenance
6. Wipe the surfaces of the primary reagent compartment with lint-free tissues
or gauze saturated with water.
7. If reagent has spilled onto the reagent pack holders, wipe the reagent pack
holders with lint-free tissues or gauze saturated with water.
8. Dry the primary reagent compartment.
9. Select Turn System Mechanics On.
10. When the system is in the Ready state, return the reagent packs to the primary
reagent compartment.
NOTE: Reagent mixing does not occur when System Mechanics are off.
Some settling of the Solid Phase reagents can occur if the reagents have not
been mixed for 2 hours.
11. Ensure that reagents are resuspended before resuming normal operation.
Refer to Appendix C, Handling Reagents in the ADVIA Centaur XP Assay
Manual for information on checking for a Solid Phase pellet and mixing
reagent packs by hand.
12. Close the primary reagent door.
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WARNING
Do not handle cleaning solution without wearing protective equipment, including
gloves, laboratory coat, and safety glasses or protective face shield.
Refer to Preparing the Cleaning Solution‚ page 5-17.
1. Carefully wipe the following components with lint-free tissues or gauze
saturated with cleaning solution:
• sample entry queue
• exterior covers
• sample exit queue
• ancillary entry
• Stat entry
• pointing device
• system fluids tray
• tray under the system fluids reservoirs
• tray under the waste and water bottles
2. Rinse the components with lint-free tissues or gauze saturated with water.
WARNING
Do not handle cleaning solution without wearing protective equipment, including
gloves, laboratory coat, and safety glasses or protective face shield.
Refer to Preparing the Cleaning Solution‚ page 5-17.
1. Open the cuvette waste area door.
2. Remove any empty sample tip trays and covers from the tip tray bin.
3. With a Phillips screwdriver, remove the screw at the left of the tip tray waste.
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4. Lift the tip tray bin up and then out of the system.
5. Remove the cuvette waste bin, empty it, and set it aside.
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6. Remove the cuvette waste chute by releasing it from the magnetic fitting.
Slightly rotate the cuvette waste chute, then pull it out and down.
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b. Wipe the reservoir with lint-free tissues or gauze saturated with cleaning
solution.
c. Rinse the reservoir with lint-free tissues or gauze saturated with reagent
water.
WARNING
Do not splatter yourself or work area with cleaning solution. The cleanins solution
is a biohazardous material and must be used carefully. Scrub the cuvette waste
chute gently to avoid splattering yourself and the work area.
9. Clean the cuvette waste chute:
a. Use the large maintenance brush and cleaning solution to gently clean the
chute.
b. Rinse the chute with water.
c. Dry the outside of the chute with paper towels.
CAUTION
Do not pinch or trap cables or tubing when installing the chute. Pinching the
cables or tubing can cause system errors.
10. Install the cuvette waste chute:
a. Place the locator pins at the bottom of the chute in the reservoir.
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b. Push up the top of the chute until it attaches to the magnetic fitting.
1 Magnet fitting
2 Locator pins
3 Cuvette waste reservoir
c. Rinse the reservoir with lint-free tissues or gauze saturated with reagent
water.
WARNING
Do not splatter yourself or work area with cleaning solution. The cleanins solution
is a biohazardous material and must be used carefully. Scrub the cuvette waste
chute gently to avoid splattering yourself and the work area.
11. Clean the cuvette waste chute:
a. Use the large maintenance brush and cleaning solution to gently clean the
chute.
b. Rinse the chute with water.
c. Dry the inside and the outside of the chute with paper towels.
CAUTION
Do not pinch or trap cables or tubing when installing the chute. Pinching the
cables or tubing can cause system errors.
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ADVIA Centaur XP Operator’s Guide: Performing Maintenance 5-49
13. Ensure that no wires or cables are interfering with the correct positioning of
the cuvette waste chute.
BIOHAZARD
Wear personal protective equipment. Use universal precautions. Refer to Safety
Instructions‚ page A-1 for recommended precautions when working with
biohazardous materials.
WARNING
Do not handle cleaning solution without wearing protective equipment, including
gloves, laboratory coat, and safety glasses or protective face shield.
Refer to Preparing the Cleaning Solution‚ page 5-17.
1. At the workspace, select System Status.
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g. Wipe the cleaning solution tubing with fresh lint-free tissues or gauze to
remove excess cleaning solution from the outside and tip of the tubing.
WARNING
Do not handle cleaning solution without wearing protective equipment, including
gloves, laboratory coat, and safety glasses or protective face shield.
6. Remove the sample tip waste chute by pulling it up and out.
WARNING
Do not splatter yourself or work area with cleaning solution. The cleanins solution
is a biohazardous material and must be used carefully. Scrub the cuvette waste
chute gently to avoid splattering yourself and the work area.
7. Unscrew the sample tip waste tube from the sample tip remover.
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8. Soak the sample tip remover, sample tip waste tube, and sample tip waste
chute in cleaning solution for 5 minutes.
9. Clean the sample tip waste reservoir:
a. While the sample tip remover, sample tip waste tube, and sample tip
waste chute are soaking, wipe the sample tip waste reservoir with lint-free
tissues or gauze saturated with cleaning solution.
b. Wipe the sample tip waste reservoir with lint-free tissues or gauze
saturated with water.
c. Dry the sample tip waste reservoir with lint-free tissues or gauze.
10. Clean the sample tip remover, sample tip waste tube, and sample tip waste
chute:
a. Use the large maintenance brush to clean the inside of the sample tip
waste tube and sample tip waste chute.
b. Rinse the sample tip remover, sample tip waste tube, and sample tip waste
chute with water.
c. Set the sample tip waste tube and sample tip waste chute aside to dry
completely.
d. Thoroughly dry the sample tip remover.
e. Connect the sample tip remover to the spare sample tip waste tube.
11. Install the sample tip remover and sample tip waste tube:
a. With the slot on the sample tip remover facing away from you, place the
bottom of the sample tip waste tube into the opening below the sample
probe.
b. Align the 2 locator pins on the right side with the holes on the mounting
block.
c. Turn the knurled knob until the sample tip remover is secure.
12. Close and secure the top cover.
CAUTION
Use care installing the sample tip waste chute. If the sample tip waste chute is not
installed correctly on the reservoir or is installed upside down, tip jams can result.
Install the chute so that the bottom fits completely in the sample tip waste
reservoir.
13. Align the pins on top of the sample tip waste chute with the hooks inside
the system, and install the bottom of the chute in the reservoir.
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ADVIA Centaur XP Operator’s Guide: Performing Maintenance 5-53
14. Ensure the wires do not interfere with the sample tip waste chute.
1 Reservoir
2 Sample tip waste chute
3 Pin
4 Hook
5 Sample tip waste area wires
BIOHAZARD
Wear personal protective equipment. Use universal precautions. Refer to Safety
Instructions‚ page A-1 for recommended precautions when working with
biohazardous materials.
1. Loosen the screw on the left side of the system that secures the top cover and
then open the top cover.
2. At the workspace, select System Status.
078D0473-03 Rev. A
5-54 ADVIA Centaur XP Operator’s Guide: Performing Maintenance
WARNING
Use care when working with the reagent probes and ancillary probe. The probe
tips are sharp and can cause skin punctures.
b. Slide the retaining clip back and remove the waste probe from the
luminometer.
078D0473-03 Rev. A
ADVIA Centaur XP Operator’s Guide: Performing Maintenance 5-55
1 Nut
2 Luminometer cover
3 Waste probe
4 Cuvette ejector rod
5. Wipe the probe and the cuvette ejector rod with gauze moistened with water.
6. Dry the waste probe and cuvette ejector rod with lint-free tissue or gauze.
7. Install the luminometer cover but do NOT replace the nut.
8. With the cover ajar, reinstall the waste probe so it sits on the white collar in
the luminometer.
9. Slide the retaining clip back to lock the probe in position.
10. Ensure that the luminometer cover is securely in place and hand-tighten the
nut.
11. Install the reagent probe cover.
12. Close and secure the top cover.
13. Select Turn System Mechanics On.
14. Dry the components thoroughly.
078D0473-03 Rev. A
5-56 ADVIA Centaur XP Operator’s Guide: Performing Maintenance
BIOHAZARD
Wear personal protective equipment. Use universal precautions. Refer to Safety
Instructions‚ page A-1 for recommended precautions when working with
biohazardous materials.
1. If the sample racks are not labeled, position a sample rack identification
barcode label on the rack.
The A label indicates multiple tube types. The vertical numbers—2, 3, and
4—on the sample rack identification barcode label correspond to the
tube-type
NOTE: If you are using ADVIA Centaur racks, ensure that you use
ADVIA Centaur labels—1, 2, 3, and 4— for the Tube-Type Selector and use
the correct barcode.
a. Firmly place the sample rack identification barcode label on the right side
of the rack and carefully wrap the label around to the front of the rack.
078D0473-03 Rev. A
ADVIA Centaur XP Operator’s Guide: Performing Maintenance 5-57
b. Ensure that the sample rack identification barcode label does not
cover the indents on the front left side of the rack.
c. Ensure that you do not have duplicate sample rack identification
numbers.
1 Calibrator label
2 Quality control label
The Cal and QC labels remind you where to place the tube-type selector to match
the calibrator or QC material tube type in the sample rack. Refer to the
Setup – Tube Type & Barcode window to verify the tube-type positions specified
for your system.
078D0473-03 Rev. A
5-58 ADVIA Centaur XP Operator’s Guide: Performing Maintenance
On the front of the sample rack identification barcode label, apply a Cal or QC
label at the position corresponding to the tube type you use for calibrators or QC
material.
For the remaining racks, you can use the tube-type labels to represent the 4 types
of tubes you specified at the Setup – Tube Type & Barcode window. The vertical
numbers, 1 to 4, on the sample rack identification barcode label correspond to the
4 tube-type positions specified at the Setup – Tube Type & Barcode window.
For example, if you select Large Transfer as Tube Type 1, you can select 1 of the
tube type labels to represent large transfer tubes.
You can use the blank labels to create your own tube-type labels or color-coded
system. Use a permanent marker to write on the blank labels.
1. Apply the tube-type labels to the front of the sample rack identification
barcode label, matching each tube-type label to the tube type position you
specified at the Setup – Tube Type & Barcode window.
For example, if Tube Type 1 is Large Transfer, place the tube-type label you
selected to represent large transfer tubes at position 1 on the sample rack
identification barcode label. The tube-type labels remind you where the
tube-type selector should be located to match the type of tube you place in the
sample rack.
2. On the front of the sample rack, align the top and left edge of the first
tube-type label with the top and left edge of the sample rack identification
barcode label.
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ADVIA Centaur XP Operator’s Guide: Performing Maintenance 5-59
3. Without overlapping the labels, position the next tube-type label directly
below the previous tube-type label.
Up to 4 tube-type labels can be placed on the sample rack identification
barcode label.
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5-60 ADVIA Centaur XP Operator’s Guide: Performing Maintenance
c. Ensure that the tube-type selector does not cover the sample rack
identification barcode number.
1 Clip
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ADVIA Centaur XP Operator’s Guide: Performing Maintenance 5-61
078D0473-03 Rev. A
5-62 ADVIA Centaur XP Operator’s Guide: Performing Maintenance
078D0473-03 Rev. A
ADVIA Centaur XP Operator’s Guide: Identifying System Problems
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ADVIA Centaur XP Operator’s Guide: Identifying System Problems
078D0473-03 Rev. A
ADVIA Centaur XP Operator’s Guide: Identifying System Problems
078D0473-03 Rev. A
ADVIA Centaur XP Operator’s Guide: Identifying System Problems
078D0473-03 Rev. A
Identifying System Problems
The ADVIA Centaur XP system provides features to help you identify and correct
system problems:
• System Status provides a visual indicator that a component or subsystem has a
warning or critical condition.
• The event log provides information about system errors and accesses
information about possible causes and corrective action procedures.
• Diagnostic tools allow you to test the operation of system components and
move system components when you perform corrective action procedures.
• Remote access enables file accessibility through a modem or through
RealTime Solutions service for technical assistance.
078D0473-03 Rev. A
6-2 ADVIA Centaur XP Operator’s Guide: Identifying System Problems
078D0473-03 Rev. A
ADVIA Centaur XP Operator’s Guide: Identifying System Problems 6-3
b. Select Comments.
c. At the Comments window, enter a comment.
You can enter up to 3 comments. You can enter comments of up to
120 alphanumeric characters. The system enters your initials and the date.
If you are not currently signed in, you must sign into the system.
d. Select Save.
If the procedure does not correct the problem or if the problem occurs again, call
for technical assistance.
If you see this event, write down the event code or print the window and then call
for technical assistance.
CAUTION
Do not disconnect the system. Disconnecting the system turns off the thermal
components of the primary reagent compartment and ancillary queue. Shut down
the system instead.
078D0473-03 Rev. A
6-4 ADVIA Centaur XP Operator’s Guide: Identifying System Problems
078D0473-03 Rev. A
ADVIA Centaur XP Operator’s Guide: Identifying System Problems 6-5
CAUTION
Do not disconnect the system. Disconnecting the system turns off the thermal
components of the primary reagent compartment and ancillary queue. Shut down
the system instead.
3. Check for obstructions in the following areas before rebooting the system:
078D0473-03 Rev. A
6-6 ADVIA Centaur XP Operator’s Guide: Identifying System Problems
• rack loader
• rack exit pusher
• sample tip loader
• ancillary entry
4. Press the green computer startup button.
WARNING
Ensure that you are clear of subassemblies that can move before selecting
Perform. The system moves subassemblies and can cause injury.
WARNING
Ensure that you are clear of subassemblies that can move before closing the
System – Diagnostic Tools window. The system returns subassemblies to their
home positions and can cause injury.
078D0473-03 Rev. A
ADVIA Centaur XP Operator’s Guide: Identifying System Problems 6-7
CAUTION
Do not leave the system on if the power is disrupted for an extended period of
time. Leaving the power on could cause serious damage to the system when the
power is restored.
078D0473-03 Rev. A
6-8 ADVIA Centaur XP Operator’s Guide: Identifying System Problems
If the power disruption is so brief that you do not have the opportunity to turn
the main power switch off, reset the system.
1. Turn the main power switch off.
078D0473-03 Rev. A
ADVIA Centaur XP Operator’s Guide: Identifying System Problems 6-9
CAUTION
Do not leave the top cover open when you add cuvettes to the cuvette loading bin.
Leaving the top cover open allows cuvettes to fall into another part of the system
where it may obstruct normal operation. Close the top cover when adding cuvettes
to the cuvette loading bin.
BIOHAZARD
Wear personal protective equipment. Use universal precautions. Refer to Safety
Instructions‚ page A-1 for recommended precautions when working with
biohazardous materials.
078D0473-03 Rev. A
6-10 ADVIA Centaur XP Operator’s Guide: Identifying System Problems
4. Check to see if the cuvettes are stacked in the bin and are preventing loading.
You can mix the cuvettes in the bin until they are no longer stacked.
5. Inspect the cuvettes in the bin and remove any damaged or deformed cuvettes.
A cuvette can be damaged and contaminated if it was dropped, stepped on,
and then loaded into the cuvette loading bin.
A deformed cuvette can have a string of plastic coming off of it or be
imperfectly molded.
6. Notify your technical representative if you find many deformed cuvettes.
7. Loosen the screw on the left side of the system that secures the top cover and
then open the top cover.
078D0473-03 Rev. A
ADVIA Centaur XP Operator’s Guide: Identifying System Problems 6-11
8. Remove and discard all cuvettes from the orientation chute and vertical
cuvette chute using a disposable transfer pipette.
078D0473-03 Rev. A
6-12 ADVIA Centaur XP Operator’s Guide: Identifying System Problems
1 Cuvette
2 Bottom window
10. Look for the cuvette at the bottom window of the vertical cuvette chute.
11. If the cuvette does not appear in the bottom window, there is an obstruction
in the vertical cuvette chute.
078D0473-03 Rev. A
ADVIA Centaur XP Operator’s Guide: Identifying System Problems 6-13
1 Thumbscrew
078D0473-03 Rev. A
6-14 ADVIA Centaur XP Operator’s Guide: Identifying System Problems
13. Manually move the conveyor upward and look for binding.
1 Conveyor slats.
2 Cuvette Conveyor
WARNING
Ensure that you are clear of subassemblies that can move before selecting
Perform. The system moves subassemblies and can cause injury.
078D0473-03 Rev. A
ADVIA Centaur XP Operator’s Guide: Identifying System Problems 6-15
078D0473-03 Rev. A
6-16 ADVIA Centaur XP Operator’s Guide: Identifying System Problems
078D0473-03 Rev. A
ADVIA Centaur XP Operator’s Guide: Identifying System Problems 6-17
1 Cuvette position.
2 Preheater
Figure 6-11
078D0473-03 Rev. A
6-18 ADVIA Centaur XP Operator’s Guide: Identifying System Problems
CAUTION
Do not turn the knob for the rotor until you move the waste probe up. Turning the
knob before moving the waste probe can damage the waste probe.
6. Move the waste probe to the up position.
078D0473-03 Rev. A
ADVIA Centaur XP Operator’s Guide: Identifying System Problems 6-19
7. Turn the knob on the left bottom of the luminometer and listen for cuvettes
dropping into the waste bin.
1 Knob
8. If the cuvettes do not drop into the waste bin and the luminometer does not
turn, check for obstructions in the cuvette waste chute and clean the cuvette
waste chute.
9. Remove the black elevator cover by loosening the thumbscrew.
078D0473-03 Rev. A
6-20 ADVIA Centaur XP Operator’s Guide: Identifying System Problems
10. If a cuvette dropped into the elevator as you were rotating the luminometer,
remove the cuvette.
1 Thumbscrew
11. Put your finger into the luminometer through the elevator and check for
evidence of fluid in the luminometer.
If your glove is wet, call for technical assistance.
12. Install the elevator cover, ensuring that the left side is flush with the edge of
the elevator.
13. Verify that the luminometer is operating correctly:
a. Select Turn System Mechanics On.
b. At the workspace, select System Status.
c. Select Diagnostic Tools.
d. At the System – Diagnostic Tools window, select Home Luminometer.
WARNING
Ensure that you are clear of subassemblies that can move before selecting
Perform. The system moves subassemblies and can cause injury.
e. Select Perform.
f. If the error persists, select Dark Count with Cuvettes.
078D0473-03 Rev. A
ADVIA Centaur XP Operator’s Guide: Identifying System Problems 6-21
g. Select Perform.
WARNING
Do not close the System – Diagnostic Tools window until you are clear of
subassemblies that can move. The system returns subassemblies to their home
positions and can cause injury.
14. Close the System – Diagnostic Tools window.
15. Install the reagent probes cover.
16. Close and secure the top cover.
17. Return to normal operation.
18. If the error persists, call for technical assistance.
Replacing Probes
This section contains procedures for replacing these probes:
• ancillary probe
• reagent probes
• aspirate probe
• waste probe
WARNING
Use care when working with the reagent probes and ancillary probe. The probe
tips are sharp and can cause skin punctures.
1. Loosen the screw on the left side of the system that secures the top cover and
then open the top cover.
078D0473-03 Rev. A
6-22 ADVIA Centaur XP Operator’s Guide: Identifying System Problems
1 Top gasket
2 Bottom gasket
3 Sleeve
4 Probe
078D0473-03 Rev. A
ADVIA Centaur XP Operator’s Guide: Identifying System Problems 6-23
WARNING
Ensure that you are clear of subassemblies that can move before selecting
Perform. The system moves subassemblies and can cause injury.
d. Select Perform.
e. Observe the ancillary probe as it moves.
If the probe movement is binding, check for interference from tubing
or wiring.
WARNING
Do not close the System – Diagnostic Tools window until you are clear of
subassemblies that can move. The system returns subassemblies to their home
positions and can cause injury.
12. Close the System – Diagnostic Tools window.
13. Close and secure the top cover.
14. Return to normal operation.
15. If the error persists, call for technical assistance.
078D0473-03 Rev. A
6-24 ADVIA Centaur XP Operator’s Guide: Identifying System Problems
4. Remove the reagent probes cover by lifting the cover up and then out.
WARNING
Use care when working with the reagent probes and ancillary probe. The probe
tips are sharp and can cause skin punctures.
5. Push the tubing off of the top of the reagent probe.
6. Loosen the probe sleeve.
078D0473-03 Rev. A
ADVIA Centaur XP Operator’s Guide: Identifying System Problems 6-25
1 Tubing
2 Probe sleeve
3 Probe
7. Pull the probe down and remove the old reagent probe and sleeve from the
holder.
8. Remove the old probe from the sleeve and discard it in a container approved
for sharp objects.
9. Install a new reagent probe.
10. Tighten the probe sleeve using the pliers.
11. Connect the tubing.
12. Select Turn System Mechanics On.
13. Verify that the probe is operating correctly:
a. At the workspace, select System Status.
b. Select Diagnostic Tools.
c. At the System – Diagnostic Tools window, select Dispense Test.
078D0473-03 Rev. A
6-26 ADVIA Centaur XP Operator’s Guide: Identifying System Problems
WARNING
Ensure that you are clear of subassemblies that can move before selecting
Perform. The system moves subassemblies and can cause injury.
e. Select Perform.
f. Ensure that the fluid dispense is steady and straight, with no sputtering.
g. Ensure that there are no leaks at the tubing connection.
WARNING
Do not close the System – Diagnostic Tools window until you are clear of
subassemblies that can move. The system returns subassemblies to their home
positions and can cause injury.
14. Close the System – Diagnostic Tools window.
15. Close and secure the top cover.
16. Install the reagent probes cover.
17. Return to normal operation.
18. If the error persists, call for technical assistance.
BIOHAZARD
Wear personal protective equipment. Use universal precautions. Refer to Safety
Instructions‚ page A-1 for recommended precautions when working with
biohazardous materials.
1. Loosen the screw on the left side of the system that secures the top cover and
then open the top cover.
2. At the workspace, select System Status.
3. Select Turn System Mechanics Off.
WARNING
Use care when working with the aspirate probe. The probe tips are sharp and can
cause skin punctures.
078D0473-03 Rev. A
ADVIA Centaur XP Operator’s Guide: Identifying System Problems 6-27
1 Probe clip
2 Aspirate probe
3 Probe entrance port
NOTE: To prevent the aspirate probe from bending, grasp the probe with
the index finger and thumb and carefully disconnect the
grounding wire from the aspirate probe.
078D0473-03 Rev. A
6-28 ADVIA Centaur XP Operator’s Guide: Identifying System Problems
CAUTION
Ensure that you return the probe clip to its locked position to hold the probe firmly
in place. Failure to do so can cause mechanical error or poor results.
078D0473-03 Rev. A
ADVIA Centaur XP Operator’s Guide: Identifying System Problems 6-29
1 Probe clip
2 Aspirate probe
WARNING
Ensure that you are clear of subassemblies that can move before selecting
Perform. The system moves subassemblies and can cause injury.
f. Select Perform.
078D0473-03 Rev. A
6-30 ADVIA Centaur XP Operator’s Guide: Identifying System Problems
WARNING
Do not close the System – Diagnostic Tools window until you are clear of
subassemblies that can move. The system returns subassemblies to their home
positions and can cause injury.
9. Close the System – Diagnostic Tools window.
10. Return to normal operation.
11. If the error persists, call for technical assistance.
1. Loosen the screw on the left side of the system that secures the top cover and
then open the top cover.
2. At the workspace, select System Status.
3. Select Turn System Mechanics Off.
078D0473-03 Rev. A
ADVIA Centaur XP Operator’s Guide: Identifying System Problems 6-31
078D0473-03 Rev. A
6-32 ADVIA Centaur XP Operator’s Guide: Identifying System Problems
c. Carefully lift the probe up until it clears the waste probe guide and then
lift the probe forward to remove it from the probe holder without bending
it.
1 Waste probe
2 Probe clip
3 Waste tubing
078D0473-03 Rev. A
ADVIA Centaur XP Operator’s Guide: Identifying System Problems 6-33
1 Nut
2 Luminometer cover
6. Wipe the top of the waste probe guide with an alcohol prep pad.
7. Place the luminometer cover over the luminometer.
Do not secure it at this time.
NOTE: Ensure that you use the correct type of probe. The aspirate probes are
cupped at the tip. The waste probe is flat at the tip.
8. Install a new waste probe:
a. Attach the waste tubing to the new waste probe.
b. Lift up the luminometer cover slightly to insert the probe tip into the
waste probe guide.
c. Lower the luminometer cover and push the probe into place.
CAUTION
Ensure that you return the probe clip to its locked position to hold the probe firmly
in place. Failure to do so can cause mechanical error or poor results.
d. Slide the probe clip into place, locking the probe.
CAUTION
Do not pinch the base pump tubing and wiring under the luminometer cover.
Pinching the tubing or wiring can lead to malfunctions.
078D0473-03 Rev. A
6-34 ADVIA Centaur XP Operator’s Guide: Identifying System Problems
WARNING
Ensure that you are clear of subassemblies that can move before selecting
Perform. The system moves subassemblies and can cause injury.
c. Select Perform.
d. If the waste probe is binding, check the routing of the tubing and the
position of the probe clip.
WARNING
Do not close the System – Diagnostic Tools window until you are clear of
subassemblies that can move. The system returns subassemblies to their home
positions and can cause injury.
14. Close the System – Diagnostic Tools window.
15. Return to normal operation.
16. If the error persists, call for technical assistance.
078D0473-03 Rev. A
ADVIA Centaur XP Operator’s Guide: Identifying System Problems 6-35
4. Twist the sleeve on the connector clockwise to tighten the new waste bottle
fluid sensor.
078D0473-03 Rev. A
6-36 ADVIA Centaur XP Operator’s Guide: Identifying System Problems
5. Install the fluid sensor in the waste bottle and move the clamp to cover the
sensor.
078D0473-03 Rev. A
ADVIA Centaur XP Operator’s Guide: Identifying System Problems 6-37
3. Remove cuvettes and sample tips that may be obstructing the inprocess queue.
4. Manually move the inprocess queue to the right.
5. Remove the used sample tip tray from the tip tray support.
6. Lift the sample tip trays out of the sample tip loader.
7. Open the cuvette waste area door.
078D0473-03 Rev. A
6-38 ADVIA Centaur XP Operator’s Guide: Identifying System Problems
CAUTION
Do not bend the latch while removing the jammed sample trays from the sample
tip loader. Bending the latch can cause more sample tip tray jams.
1 Latch
CAUTION
Do not bend pull a jammed tray up through the top of the sample tip loader.
Pulling a jammed trays through the top of the sample tip loader can damage the
spring clips. Pull jammed trays down through the sample tip tray waste area.
9. If a sample tip tray is stuck in the loader, carefully pull the sample tip tray
down through the sample tip tray waste area.
NOTE: If you find a deformed sample tip or sample tip tray in the system,
notify your technical representative .
10. Load a new sample tip tray into the sample tip loader:
a. At the workspace, select System Status.
b. Select Diagnostic Tools.
c. At the System – Diagnostic Tools window, select Load Tip Tray.
WARNING
Ensure that you are clear of subassemblies that can move before selecting
Perform. The system moves subassemblies and can cause injury.
078D0473-03 Rev. A
ADVIA Centaur XP Operator’s Guide: Identifying System Problems 6-39
d. Select Perform.
11. Ensure that the sample tip tray loader and inprocess queue perform the
following sequence of events as a new tray is loaded:
• latch snaps over the new sample tip tray cover
• new sample tip tray drops down smoothly
• sample tip trays in the loader are not stuck together
• sample tip tray drops down over the pins on the tip tray support
• when the inprocess queue moves right, the sample tip tray is locked
completely onto the pins
• as the sample tip tray is pulled out of the loader when the inprocess queue
moves right, the cover stays in the loader
• the cover drops down into the tip tray waste area
• the empty sample tip tray drops smoothly down into the tip tray waste
area
WARNING
Do not close the System – Diagnostic Tools window until you are clear of
subassemblies that can move. The system returns subassemblies to their home
positions and can cause injury.
12. Close the System – Diagnostic Tools window.
13. Close and secure the top cover.
14. Return to normal operation.
15. If the error persists, call for technical assistance.
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6-40 ADVIA Centaur XP Operator’s Guide: Identifying System Problems
1. Remove all the sample racks from the sample exit queue.
078D0473-03 Rev. A
ADVIA Centaur XP Operator’s Guide: Identifying System Problems 6-41
Troubleshooting Leaks
Use this procedure if you find substantial amounts of liquid in or around
the system.
BIOHAZARD
Wear personal protective equipment. Use universal precautions. Refer to Safety
Instructions‚ page A-1 for recommended precautions when working with
biohazardous materials.
1. Press 1 of the fast stop buttons.
Figure 6-29 ADVIA Centaur XP System – front view and back view
078D0473-03 Rev. A
6-42 ADVIA Centaur XP Operator’s Guide: Identifying System Problems
CAUTION
Do not disconnect the system. Disconnecting the system turns off the thermal
components of the primary reagent compartment and ancillary queue. Shut down
the system instead.
078D0473-03 Rev. A
ADVIA Centaur XP Operator’s Guide: Identifying System Problems 6-43
WARNING
Ensure that you are clear of subassemblies that can move before selecting
Perform. The system moves subassemblies and can cause injury.
WARNING
Do not close the System – Diagnostic Tools window until you are clear of
subassemblies that can move. The system returns subassemblies to their home
positions and can cause injury.
15. Close the System – Diagnostic Tools window.
16. If the process completes successfully, resume normal operation.
Diagnostic Tools allow you to prime the system, test the operation of system
components, and move system components when you perform corrective action
procedures.
078D0473-03 Rev. A
6-44 ADVIA Centaur XP Operator’s Guide: Identifying System Problems
Empty Ring
Use this test to empty the cuvettes from the incubation ring.
During this test, the system performs the following actions:
• checks that the cuvette waste bin can accept another 115 cuvettes
• homes all subassemblies
• moves a cuvette into the luminometer and then the incubation ring indexes
to advance 1 cuvette
The system repeats these steps until all cuvettes are out of the incubation ring.
During this test, the cuvette pusher is not active.
You can observe that the system correctly empties cuvettes:
1. Loosen the screw on the left side of the system that secures the top cover and
then open the top cover.
2. At the workspace, select System Status.
3. Select Diagnostic Tools.
4. At the System – Diagnostic Tools window, select Empty Ring.
WARNING
Ensure that you are clear of subassemblies that can move before selecting
Perform. The system moves subassemblies and can cause injury.
078D0473-03 Rev. A
ADVIA Centaur XP Operator’s Guide: Identifying System Problems 6-45
5. Select Perform.
This process takes approximately 12 minutes to complete.
6. As the system moves all cuvettes out of the incubation ring, watch and listen
as the incubation ring, elevator, and luminometer move.
7. If cuvettes are jamming, check the event log for related errors and then correct
the problem or call for technical assistance.
8. If the process completes successfully, close and secure the top cover and then
resume normal operation.
WARNING
Do not close the System – Diagnostic Tools window until you are clear of
subassemblies that can move. The system returns subassemblies to their home
positions and can cause injury.
9. Close the System – Diagnostic Tools window.
078D0473-03 Rev. A
6-46 ADVIA Centaur XP Operator’s Guide: Identifying System Problems
You can observe that the system correctly empties cuvettes and fills the ring with
cuvettes:
1. Loosen the screw on the left side of the system that secures the top cover and
then open the top cover.
2. At the workspace, select System Status.
3. Select Diagnostic Tools.
4. At the System – Diagnostic Tools window, select Empty & Fill Ring.
WARNING
Ensure that you are clear of subassemblies that can move before selecting
Perform. The system moves subassemblies and can cause injury.
5. Select Perform.
6. As the system moves all cuvettes out of the incubation ring and loads new
cuvettes into the ring, watch and listen as the cuvette pusher, incubation ring,
elevator, and luminometer move.
This process takes approximately 12 minutes to complete.
7. If cuvettes are jamming, check the event log for related errors and then correct
the problem or call for technical assistance.
8. If the process completes successfully, close and secure the top cover and then
resume normal operation.
WARNING
Do not close the System – Diagnostic Tools window until you are clear of
subassemblies that can move. The system returns subassemblies to their home
positions and can cause injury.
9. Close the System – Diagnostic Tools window.
Prime Cuvettes
Use this test to perform the following:
• start the cuvette conveyor after the cuvette supply is replenished
• start the cuvette conveyor after you recover from a cuvette loading jam
During this test, the system performs the following actions:
• cuvette conveyor moves to fill the vertical cuvette chute with cuvettes
During this test, the cuvette pusher is not active.
You can observe that the system correctly loads cuvettes:
1. Loosen the screw on the left side of the system that secures the top cover and
then open the top cover.
2. At the workspace, select System Status.
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ADVIA Centaur XP Operator’s Guide: Identifying System Problems 6-47
WARNING
Ensure that you are clear of subassemblies that can move before selecting
Perform. The system moves subassemblies and can cause injury.
5. Select Perform.
6. Watch and listen as the cuvette conveyor moves.
The ionizer is also activated when the cuvette conveyor is operating. You
can verify that the ionizer is on by feeling for air blowing down under it as
the cuvette conveyor moves.
7. If the cuvettes are jamming, check the event log for related errors and then
correct the problem or call for technical assistance.
8. If the process completes successfully, close and secure the top cover and then
resume normal operation.
WARNING
Do not close the System – Diagnostic Tools window until you are clear of
subassemblies that can move. The system returns subassemblies to their home
positions and can cause injury.
9. Close the System – Diagnostic Tools window.
Dispense Test
Use this test to perform the following:
• observe the integrity of the stream of water dispensed into the rinse station
by ancillary and reagent probes
• verify correct installation of a new probe
• investigate vacuum or water errors
During this test, the system performs the following actions:
• homes all subassemblies
• homes all probes over the rinse stations
• turns the vacuum and the water pump on
• activates the rinse station vacuum valve
• dispenses water into the rinse station
• repeats the dispense for a selected number of repetitions
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1 Diluter drawer
2. Loosen the screw on the left side of the system that secures the top cover and
then open the top cover.
3. At the workspace, select System Status.
4. Select Diagnostic Tools.
5. At the System – Diagnostic Tools window, select Dispense Test.
6. Select the probe you want to test.
7. Select the number of times you want the probe to dispense.
WARNING
Ensure that you are clear of subassemblies that can move before selecting
Perform. The system moves subassemblies and can cause injury.
8. Select Perform.
The system homes all subassemblies.
9. Look at the stream of fluid as the system dispenses.
10. Ensure that the stream is straight and steady, with no dripping.
11. If the probe is dripping, look for a leaking valve.
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ADVIA Centaur XP Operator’s Guide: Identifying System Problems 6-49
12. If the process completes successfully, close and secure the top cover and then
resume normal operation.
WARNING
Do not close the System – Diagnostic Tools window until you are clear of
subassemblies that can move. The system returns subassemblies to their home
positions and can cause injury.
13. Close the System – Diagnostic Tools window.
Home Probes
Use this test to perform the following:
• move the 3 reagent probes, their associated diluters, and the ancillary probe
into primary positions
• identify probe move errors caused by interfering tubing or wiring
• replace a reagent or ancillary probe
• verify the installation of a new reagent or ancillary probe
• clean the reagent probe shutter
• verify the installation of the reagent probe shutter
• access the luminometer, preheater, or elevator
During this test, the reagent probe moves to the following positions:
• homes vertically
• homes horizontally toward the cuvette
• homes horizontally toward the reagent pack
• homes horizontally to its rinse station
• homes the associated diluter
This test does not home the reagent probe shutter or the reagent mixer.
During this test, the ancillary probe performs the following actions:
• homes vertically, then homes horizontally in the pack-cuvette direction and
then horizontally to its rinse station
• homes the associated diluters
You can observe that the system correctly homes the primary and ancillary
reagent probes:
1. Loosen the screw on the left side of the system that secures the top cover and
then open the top cover.
2. At the workspace, select System Status.
3. Select Diagnostic Tools.
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WARNING
Ensure that you are clear of subassemblies that can move before selecting
Perform. The system moves subassemblies and can cause injury.
5. Select Perform.
6. Watch and listen as the reagent and ancillary probes move.
7. If the probes do not move correctly, check the event log for related errors
and then correct the problem or call for technical assistance.
8. If the process completes successfully, close and secure the top cover and then
resume normal operation.
WARNING
Do not close the System – Diagnostic Tools window until you are clear of
subassemblies that can move. The system returns subassemblies to their home
positions and can cause injury.
9. Close the System – Diagnostic Tools window.
Home Probe
Use this test to perform the following:
• move the sample probe into its primary position
• investigate sample probe movement errors
During this test, the sample probe moves to the following positions:
• homes vertically
• homes the probe horizontally in the rack-to-cuvette direction
• moves the probe horizontally toward the ring
• moves down as if ejecting a tip
• moves horizontally to the tip remover
• moves up and stays above the tip remover
• sample diluter homes
You can observe that the system correctly homes the sample probe:
1. Loosen the screw on the left side of the system that secures the top cover and
then open the top cover.
2. At the workspace, select System Status.
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ADVIA Centaur XP Operator’s Guide: Identifying System Problems 6-51
WARNING
Ensure that you are clear of subassemblies that can move before selecting
Perform. The system moves subassemblies and can cause injury.
5. Select Perform.
6. Watch and listen as the sample probe and inprocess queue move.
7. If the probe does not move correctly, check the event log for related errors
and then correct the problem or call for technical assistance.
8. If the process completes successfully, close and secure the top cover and then
resume normal operation.
WARNING
Do not close the System – Diagnostic Tools window until you are clear of
subassemblies that can move. The system returns subassemblies to their home
positions and can cause injury.
9. Close the System – Diagnostic Tools window.
CAUTION
Do not leave tips on the sample tip tray on the inprocess queue when you eject the
tray. If tips are are on the tray when you eject the tray, a jam occurs. Ensure that
the sample tip tray on the inprocess queue is empty before you select this tool.
2. Remove the sample tip tray from the inprocess queue:
a. Manually move the inprocess queue so you can access the sample tip tray.
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b. Move the sample tip tray to the left to disengage the tray from the locking
pins.
WARNING
Ensure that you are clear of subassemblies that can move before selecting
Perform. The system moves subassemblies and can cause injury.
6. Select Perform.
7. Select Continue.
The system resets the counter that determines which tip to pick up next.
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8. Watch and listen as the sample tip loader loads a new sample tip tray.
9. If the sample tip tray does not load correctly, check the event log for related
errors and then correct the problem or call for technical assistance.
10. If the process completes successfully, close and secure the top cover and then
resume normal operation.
WARNING
Do not close the System – Diagnostic Tools window until you are clear of
subassemblies that can move. The system returns subassemblies to their home
positions and can cause injury.
11. Close the System – Diagnostic Tools window.
Luminometer Tools
Use the following diagnostic tools to test the luminometer.
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4. In Number of Cuvettes, select the number of cuvettes you want to use for the
test.
WARNING
Ensure that you are clear of subassemblies that can move before selecting
Perform. The system moves subassemblies and can cause injury.
5. Select Perform.
The System – Dark Count Data window opens at the completion of the test.
• If you select 8 cuvettes, the system displays values for the RLU 1
fields.
• If you select 16 cuvettes, the system displays values for the RLU 1
fields and RLU 2 fields.
• If you select 24 cuvettes, the system displays values for the RLU 1
fields, RLU 2 fields, and RLU 3 fields.
6. Record the Total Mean.
Ensure that the difference between the values for Total Mean obtained in this
test and Total Mean obtained in the Dark Count without Cuvettes test is less
than 25. RLUs higher than 25 may indicate light leaks. Refer to the procedure
Dark Count without Cuvette‚ page 6-54.
7. If the dark count test does not meet specifications, call for technical
assistance.
WARNING
Do not close the System – Diagnostic Tools window until you are clear of
subassemblies that can move. The system returns subassemblies to their home
positions and can cause injury.
8. Close the System – Diagnostic Tools window.
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ADVIA Centaur XP Operator’s Guide: Identifying System Problems 6-55
WARNING
Ensure that you are clear of subassemblies that can move before selecting
Perform. The system moves subassemblies and can cause injury.
5. Select Perform.
The System – Dark Count Data window opens at the completion of the test.
• If you select 8 cuvettes, the system displays values for the RLU 1
fields.
• If you select 16 cuvettes, the system displays values for the RLU 1
fields and RLU 2 fields.
• If you select 24 cuvettes, the system displays values for the RLU 1
fields, RLU 2 fields, and RLU 3 fields.
6. Record the Total Mean and Total SD values:
a. Ensure that the value for Total Mean is less than 600.
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RLUs higher than 600 may indicate a defective PMT, a wet or dirty
luminometer, electronic noise, or light leaks.
b. Find the Total Mean value that you obtained in the dark count test on the
x-axis and determine the corresponding Total SD specification limit from
the curve on the chart.
c. Verify that the Total SD value that you obtained in the dark count test is at
or below the Total SD specification limit.
For example, if the Total Mean value of the dark count test is 80,
according to the chart, the corresponding Total SD specification limit is
15.00. A Total SD value of 15.00 or less in the dark count test is within
specifications.
7. If the dark count test does not meet specifications, call for technical
assistance.
WARNING
Do not close the System – Diagnostic Tools window until you are clear of
subassemblies that can move. The system returns subassemblies to their home
positions and can cause injury.
8. Close the System – Diagnostic Tools window.
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Home Luminometer
Use this test to perform the following:
• move the luminometer and its associated subsystem components into their
primary positions
• investigate waste probe home sensor errors
• investigate luminometer home sensor errors
• recover from a cuvette jam in the luminometer or elevator
During this test, the system performs the following actions:
• checks and homes the elevator down position
• homes the waste probe
• moves luminometer to the next load position
• rotates and pauses luminometer at the remaining 7 positions while the cuvette
ejector rod moves down
• homes luminometer
You can observe that the system correctly homes the luminometer:
1. Loosen the screw on the left side of the system that secures the top cover and
then open the top cover.
2. At the workspace, select System Status.
3. Select Diagnostic Tools.
At the System – Diagnostic Tools window, select Home Luminometer.
WARNING
Ensure that you are clear of subassemblies that can move before selecting
Perform. The system moves subassemblies and can cause injury.
4.
5. Select Perform.
6. Listen as the luminometer moves.
7. If the luminometer does not move correctly, check the event log for related
errors and then correct the problem or call for technical assistance.
8. If the process completes successfully, close and secure the top cover and then
resume normal operation.
WARNING
Do not close the System – Diagnostic Tools window until you are clear of
subassemblies that can move. The system returns subassemblies to their home
positions and can cause injury.
9. Close the System – Diagnostic Tools window.
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Home Probes
Use this test to perform the following:
• move the aspirate probes to their primary positions (up)
If they are already up, they move down slightly and then back up.
• identify errors caused by interfering tubing or wiring
• replace or clean aspirate probes
You can observe that the system correctly homes the aspirate probes:
1. Loosen the screw on the left side of the system that secures the top cover and
then open the top cover.
2. At the workspace, select System Status.
3. Select Diagnostic Tools.
4. At the System – Diagnostic Tools window, select Home Probes.
WARNING
Ensure that you are clear of subassemblies that can move before selecting
Perform. The system moves subassemblies and can cause injury.
5. Select Perform.
6. Watch and listen as the aspirate probes move.
7. If the aspirate probes do not move correctly, check the event log for related
errors and then correct the problem or call for technical assistance.
8. If the process completes successfully, close and secure the top cover and then
resume normal operation.
WARNING
Do not close the System – Diagnostic Tools window until you are clear of
subassemblies that can move. The system returns subassemblies to their home
positions and can cause injury.
9. Close the System – Diagnostic Tools window.
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6-60 ADVIA Centaur XP Operator’s Guide: Identifying System Problems
NOTE: If the diluter drawer is not closed, the system does not prime the wash
station diluters correctly.
1. Ensure that the diluter drawer is in the closed, upright position.
1 Diluter drawer
2. Loosen the screw on the left side of the system that secures the top cover and
then open the top cover.
3. At the workspace, select System Status.
4. Select Diagnostic Tools.
5. Select Dispense Wash Fluid & Aspirate.
6. Select the dispense port and aspirate probe pair you want to test.
7. You can select the fluid you want the probe to dispense.
8. Select the number of times you want the selected probe to dispense the
selected fluid.
WARNING
Ensure that you are clear of subassemblies that can move before selecting
Perform. The system moves subassemblies and can cause injury.
9. Select Perform.
10. Watch the port dispense the fluid.
The stream should be straight and steady.
NOTE: Some dispense ports dispense fluid from 2 locations.
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• Dispense port 1 dispenses from the right and left sides of aspirate probe 1.
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• Dispense port 2 dispenses from the right and left sides of aspirate probe 2.
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• Dispense port 3 dispenses from the right and left sides of aspirate probe 3.
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• The wash displacement port dispenses from the area between aspirate
probe 2 and aspirate probe 3 and the resuspension ports are on the right
end of the manifold.
WARNING
Do not close the System – Diagnostic Tools window until you are clear of
subassemblies that can move. The system returns subassemblies to their home
positions and can cause injury.
15. Close the System – Diagnostic Tools window.
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WARNING
Ensure that you are clear of subassemblies that can move before selecting
Perform. The system moves subassemblies and can cause injury.
4. Select Perform.
5. If the subassemblies do not move correctly, check the event log for related
errors and then correct the problem or call for technical assistance.
6. If the process completes successfully, resume normal operation.
WARNING
Do not close the System – Diagnostic Tools window until you are clear of
subassemblies that can move. The system returns subassemblies to their home
positions and can cause injury.
7. Close the System – Diagnostic Tools window.
You can observe that the system correctly homes the inprocess queue:
NOTE: If the inprocess queue is in the home position, move it out of the home
position before performing this procedure.
1. Loosen the screw on the left side of the system that secures the top cover and
then open the top cover.
2. At the workspace, select System Status.
3. Select Diagnostic Tools.
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WARNING
Ensure that you are clear of subassemblies that can move before selecting
Perform. The system moves subassemblies and can cause injury.
5. Select Perform.
6. Watch and listen as the inprocess queue and sample probe move.
a. If the inprocess queue and sample probe do not move correctly, check the
event log for related errors and then correct the problem or call for
technical assistance.
b. If the process completes successfully, close and secure the top cover and
then resume normal operation.
WARNING
Do not close the System – Diagnostic Tools window until you are clear of
subassemblies that can move. The system returns subassemblies to their home
positions and can cause injury.
7. Close the System – Diagnostic Tools window.
You can evaluate if the system correctly homes the ancillary queue:
1. At the workspace, select System Status.
2. Select Diagnostic Tools.
3. At the System – Diagnostic Tools window, select Home Ancillary Queue.
WARNING
Ensure that you are clear of subassemblies that can move before selecting
Perform. The system moves subassemblies and can cause injury.
4. Select Perform.
5. Listen as the ancillary queue moves.
a. If the ancillary queue does not move correctly, check the event log for
related errors and then correct the problem or call for technical assistance.
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WARNING
Do not close the System – Diagnostic Tools window until you are clear of
subassemblies that can move. The system returns subassemblies to their home
positions and can cause injury.
6. Close the System – Diagnostic Tools window.
WARNING
Ensure that you are clear of subassemblies that can move before selecting
Perform. The system moves subassemblies and can cause injury.
5. Select Perform.
NOTE: The sample exit pusher moves even if there is no rack in that position of
the inprocess queue.
6. Watch and listen as the sample exit pusher pushes the racks out of the
inprocess queue.
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7. If the inprocess queue and sample probe do not move correctly, check the
event log for related errors and then correct the problem or call for technical
assistance.
8. If the process completes successfully, close and secure the top cover and then
resume normal operation.
WARNING
Do not close the System – Diagnostic Tools window until you are clear of
subassemblies that can move. The system returns subassemblies to their home
positions and can cause injury.
9. Close the System – Diagnostic Tools window.
WARNING
Ensure that you are clear of subassemblies that can move before selecting
Perform. The system moves subassemblies and can cause injury.
4. Select Perform.
5. Remove the ancillary pack from the ancillary entry.
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WARNING
Do not close the System – Diagnostic Tools window until you are clear of
subassemblies that can move. The system returns subassemblies to their home
positions and can cause injury.
9. Close the System – Diagnostic Tools window.
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6. If the system displayed Vacuum Low or Vacuum High, check the event log for
related errors and then correct the problem or call for technical assistance.
7. If the Vacuum Test completes successfully, resume normal operation.
8. Close the System – Diagnostic Tools window.
LASER WARNING
Never look directly at the laser beam or at its reflection from a shiny surface.
Looking directly at the laser beam or its reflection could cause damage to the
eyes. Only trained field service personnel should perform procedures related to
laser assemblies. Refer to Appendix A, Protecting Yourself from Barcode Scanner
Lasers, for more information.
WARNING
Ensure that you are clear of subassemblies that can move before selecting
Perform. The system moves subassemblies and can cause injury.
5. Select Perform.
Wait until the System – Sample Barcode Read Test window opens with the
test results.
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6. If the test results are unacceptable, check the event log for related errors and
then correct the problem or call for technical assistance.
7. If the test results are acceptable, return to normal operation.
WARNING
Do not close the System – Diagnostic Tools window until you are clear of
subassemblies that can move. The system returns subassemblies to their home
positions and can cause injury.
8. Close the System – Diagnostic Tools window.
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NOTE: If the diluter drawer is not closed (up), the system does not prime the
wash station diluters correctly.
1. Ensure that the diluter drawer is in the closed, upright position.
1 Diluter drawer
WARNING
Ensure that you are clear of subassemblies that can move before selecting
Perform. The system moves subassemblies and can cause injury.
5. Select Perform.
6. If the system does not prime correctly, check the event log for related errors
and then correct the problem or call for technical assistance.
7. If the process completes successfully, resume normal operation.
WARNING
Do not close the System – Diagnostic Tools window until you are clear of
subassemblies that can move. The system returns subassemblies to their home
positions and can cause injury.
8. Close the System – Diagnostic Tools window.
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1 Diluter drawer
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ADVIA Centaur XP Operator’s Guide: Identifying System Problems 6-75
WARNING
Ensure that you are clear of subassemblies that can move before selecting
Perform. The system moves subassemblies and can cause injury.
8. Select Perform.
9. You can observe the diluters while the system is performing the test:
a. Access the area of the system where you suspect a problem:
To test the... Perform these steps...
sample or ancillary loosen the screw on the left side of the system that
diluters, secures the top cover and then open the top cover.
reagent probe diluters, loosen the 2 thumbscrews on the left side of the system
and then lift the cover up and off.
other system diluters, open the diluter drawer to observe the diluters move.
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1 From left to right: Reagent Probe 3 Diluter, Reagent Probe 2 Diluter, Reagent
Probe 1 Diluter
2 From left to right: Sample Diluter, Ancillary Diluter
3 From left to right: Resuspend Dispense Diluter, Dispense 1 Diluter, Dispense 2
Diluter, Wash Displacement Diluter, Dispense 3 Diluter
WARNING
Do not close the System – Diagnostic Tools window until you are clear of
subassemblies that can move. The system returns subassemblies to their home
positions and can cause injury.
12. Close the System – Diagnostic Tools window.
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WARNING
Ensure that you are clear of subassemblies that can move before selecting
Perform. The system moves subassemblies and can cause injury.
5. Select Perform.
6. If the fluid does not prime correctly, check the event log for related errors
and then correct the problem or call for technical assistance.
7. If the process completes successfully, resume normal operation.
WARNING
Do not close the System – Diagnostic Tools window until you are clear of
subassemblies that can move. The system returns subassemblies to their home
positions and can cause injury.
8. Close the System – Diagnostic Tools window.
Remote Access
If you are not using the RealTime Solutions service and your system is connected
to a modem, select remote access to make your files accessible for technical
assistance. This feature allows Siemens Medical Solutions Diagnostics to collect
SID and RLU data. Refer to Selecting Remote Access‚ page 8-42.
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Use the following procedures to resolve problems with the hand-held barcode
scanner.
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2. For the check digit option, at the Setup – Tube Type & Barcode window,
select Disable.
3. Check the barcode scanner, cable, and port.
4. If the problem persists, call for technical assistance.
Use the following procedures to resolve problems with the sample barcode
scanner.
4. Ensure that the sample racks load smoothly into the inprocess queue.
5. Ensure that the upright panel behind the sample entry queue is installed.
6. The panel prevents the barcode scanner from reading racks that are already
loaded in the inprocess queue.
7. If the problem persists, call for technical assistance.
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1. Ensure that the sample barcodes do not have quality defects such as faded
or inconsistent print.
Refer to Barcode Quality‚ page 6-85.
2. Ensure that the barcode labels are positioned correctly on the sample rack
and the sample tubes or cups.
3. Ensure that the information on the Setup – Tube Type & Barcode window
is correct.
CAUTION
Do not press the sample start button until you close the primary reagent door and
ensure that all reagent barcodes were read correctly. Starting the system before all
reagent barcodes are read correctly can cause erroneous results. Closing the
primary reagent door allows the system barcode scanner to rescan the reagent
barcodes and acknowledge any changes.
4. Press the sample start button and then look for a red light reflection on the
rack as the scanner reads the barcode label.
5. If you cannot determine the cause, you can enter the SID at the Status – Enter
SID window.
6. If the problem persists, call for technical assistance.
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Use the following procedure to resolve problems with the reagent barcode
scanner.
When the system cannot read the primary pack barcode, the system displays the
Status – Enter Primary Barcodes window. You can manually enter the barcode in
the appropriate field.
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To determine why the system could not read the barcode, use the following tips. If
the problem persists, contact your local technical support provider or distributor.
• Ensure that the reagent packs are seated correctly on the reagent shelf in the
primary reagent compartment.
• If only 1 pack is not being read, move it to another position in the primary
reagent compartment and then notify your field service representative.
• Wipe off any condensation from the reagent pack labels and the reagent shelf.
• If the barcode labels are not correctly placed on the reagent packs, call for
technical assistance.
• Ensure that the reagent barcodes do not have quality defects such as faded
or inconsistent print.
Refer to Barcode Quality‚ page 6-85.
• Open and close the primary reagent door and then look for a red light
reflection on the back of the primary reagent compartment as the scanner
reads the barcode labels.
• Ensure that the reagent barcode reader moves and that the reagent barcode
shutter opens.
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If you are unable to resolve the problem, manually enter the barcode at the
Status – Enter Primary Barcode window. Contact your local service
provider about the problem.
Use the following procedure to resolve problems with the ancillary barcode
scanner.
When the system cannot read the ancillary pack barcode, the system displays the
Status – Enter Ancillary Barcodes window. You can manually enter the barcode in
the appropriate field.
To determine why the system could not read the barcode, use the following tips. If
the problem persists, contact your local technical support provider or distributor.
• Check the ancillary pack labels for condensation.
• Ensure that the barcode labels are positioned correctly on ancillary packs.
• Ensure that the ancillary barcodes do not have quality defects such as faded or
inconsistent print.
Refer to Barcode Quality‚ page 6-85.
• Place an ancillary pack in the ancillary entry and then look for a red light
reflection as the scanner reads the barcode label.
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The ability of the ADVIA Centaur XP system to scan a barcode can be affected by
the following barcode characteristics:
• size
• color
• quality
• positioning
• symbology
• fixed or variable length
• check digit
• reserved characters
Barcode Size
The required dimensions of the label and the barcode are listed below:
Dimension Size (cm) Size (in)
maximum label length 8.89 3.50
quiet zone at each end 0.64 0.25
maximum barcode length 7.60 3.00
minimum barcode height 0.64 0.25
minimum narrow bar width 0.02 0.01
Barcode Color
Since barcode scanners read at a visible wavelength, the preferred background
color is white. The preferred print color of symbols is black. Use of other colors
can affect the ability of the system to read the barcode.
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Barcode Quality
The ideal barcode label has clean, clear, straight fine lines with high contrast
between light and dark areas.
Problems with the barcode label that may interfere with scanning can include
problems with the material or surface on which barcodes are printed, poor quality
printing, environmental factors that damage the barcodes during storage or use,
and incorrect placement of the barcode label on the sample tube or sample cup.
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The following table contains the quality components and factors causing scanning
problems:
Quality Components Factors Causing Scanning Problems
environmental factors during abrasion
storage and use aging
chemicals
frost
fungi and mildew
humidity
lubricants
packaging preservatives
protective coatings
salt spray
solvents and cleaners
tape
temperature
ultraviolet light
label material conspicuous grain
high porosity
low opacity
label surface coating or overlaminate too thick (> 0.076 mm
or 0.003 in)
high gloss laminates
highly textured laminates
high matte laminates
polarized laminates
very coarse surface
very smooth surface
placement of label on sample cup crooked
print quality faded areas
irregularities
low scan or symbol contrast
no leading or trailing quiet zone
scratches
smears
smudges
streaks
very dark or very light barcodes
very thin or thick lines
voids
wavy, slanted, or curved lines
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Barcode Positioning
Position the barcode vertically on the primary sample tube or sample cup,
approximately 2 cm (0.8 in) from the top. If the barcode label is twisted, the
barcode scanner might not read the label. Ensure that the labels are placed firmly
on the sample cup. It is recommended that you do not place more than 2 labels on
a primary sample tube or sample cup.
Barcode Symbology
At the Setup – Tube Type and Barcode window, select the appropriate
symbologies:
• Codabar
• Interleaved 2 of 5
• Code 39
• Code 128
Code 128 is the system default symbology and is always active since it is used for
all Siemens labels for sample racks, primary reagent packs, ancillary reagent
packs, calibrators, and controls.
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This information does not apply to the master curve, calibrator, and control
information that is read by the barcode scanner.
1. At the Workspace, select Setup.
2. Select Summary.
3. Select Tube Type & Barcode.
4. For each active symbology, select the appropriate Check Digit option:
• Enable
• Disable
• nw7 (Codabar standard check digits)
• mod16 (Codabar standard check digits)
• nw7mod16 (Codabar standard check digits)
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The barcode scanner is active after selecting Scan at the Calibration – Master
Curve Definition window or when the Calibration – Calibrator Definition window
is open. Use the following techniques when scanning a barcode:
• Center the barcode scanner over the barcode.
• Move the barcode scanner from the top of the card to the bottom of the card.
• Ensure that the system beeps after scanning a barcode before proceeding to
the next barcode.
• Ensure that the tip of the scanner is directly contacting the barcode and that
you are not pressing down so hard that you mar the barcode.
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• Defined ranges are specified for each lot at the time of reagent
manufacture.
• Observed ranges are established by the system.
7. Determine if the values for slope, ratio, low cal deviation, or high cal
deviation are within the defined or observed ranges.
If the value is... Then...
out of the defined range use the tables that follow to evaluate flagged calibration
metrics for sandwich or competitive assays.
out of the observed you can accept the calibration. Accepting an
range but within the invalid calibration due to a failed observed range
defined range is acceptable when parts have been replaced on the system,
such as probes, tubing, or filters. Refer to Accepting an
Invalid Calibration‚ page 3-25.
Refer to Understanding Calibration Evaluation Criteria‚ page 3-4.
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Discrepant QC Results
Possible Cause Corrective Actions
The controls were 1. Ensure that the correct volume was used for
reconstituted reconstitution. Refer to the control package insert
incorrectly: with tap for reconstitution instructions.
water, with the incorrect 2. Ensure that the pipette is calibrated correctly.
volume, with an
uncalibrated pipette, or 3. Check the integrity of the reagent water.
with contaminated Refer to Reagent Water Quality‚ page C-4.
reagent water 4. Reconstitute fresh controls.
5. Perform quality control again.
The controls were on 1. Ensure that controls are discarded after being on
the system and then the system and not poured back into the respective
were poured back into bottles.1
the bottle
2. Pour a fresh aliquot of controls.
3. If necessary, reconstitute fresh lyophilized controls
or open fresh liquid controls.
4. Perform quality control again.
Incomplete control 1. Check the control vial for uniform reconstitution.
equilibration after 2. Check the control package insert for control
reconstitution preparation instructions.
3. If necessary, reconstitute fresh lyophilized controls
or open fresh liquid controls.
4. Perform quality control again.
The controls were not 1. Check the control package insert for the storage
stored correctly requirements of lyophilized, reconstituted, and liquid
controls.
2. If, according to the control package insert, controls
can be aliquotted and frozen, then mix controls
gently by inversion to ensure a homogeneous
solution before aliquotting.
3. If the storage temperature is outside the
recommended temperature range, then reconstitute
fresh lyophilized controls or open fresh liquid
controls that have been stored correctly.
4. Perform quality control again.
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If you have had prior issues with proficiency testing results or have any questions
or concerns, call for technical assistance before you submit your results. To help
the product specialist in assisting you, be sure to have the following information
available:
• Obtain calibration and control data from the day of testing, including lot
number and expiration date.
• Check all control results and determine if they are within acceptable limits.
• Ensure that all survey sample preparation procedures have been followed.
• Note any unusual occurrences such as error flags and non-reproducible
results.
• Check that reagent and instrument reporting categories have been entered
correctly.
References
1. Kaplan, Lawrence A. Pesce, Amadeo J. Clinical Chemistry; theory, analysis,
and correlation, 2nd edition. St. Louis: C.V. Mosby Company; 1989. 1212 p.
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7 Managing Data
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CAUTION
Do not place the storage medium on the sample entry queue. The magnets under
the queue can erase the information on the storage medium.
Restoring Data
CAUTION
Do not perform the Restore function. Performing the Restore function incorrectly
can cause corruption of the system software. To avoid potential corruption of the
system software, only a trained technical support provider should perform the
Restore function at the Setup – Data Administration window.
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Archived Data
This table displays the field name and field format that is displayed in the archive
spreadsheet. Explanatory comments are included, where necessary.
The field name is the column head of the spreadsheet and the field format is how
the data is displayed. Data is sorted in the spreadsheet in ascending order by the
SID with a secondary sort on the Result Date and Time.
Autoscheduled archive files and archives created by selecting Archive All on the
Status–Archive Options window are named using the following format:
A_RA_<system serial number>_<date><time>
Field Name Field Format Comment
SID Alphanumeric; Sample identification for
maximum of 13 characters patient, control, or calibrator
samples
Rack Alphanumeric; First 4 digits is the Rack ID
5 characters Fifth character is the
Position
Example: 0001A
Result Date and Time DD MMM YY HH:MM —
Patient Name Last Name, First Name; —
30 characters maximum
PID Alphanumeric; —
11 characters maximum
Sex M, F, or U —
Test Name Alphanumeric; —
8 characters maximum
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NOTE: For autoschedule archives, you must ensure that the selected storage
device is in place for the scheduled archive event.
• Frequency: Enter a number from 1 – 31 to indicate how many days between
archive events.
The default is 7.
• Time: Enter a time of day for the archive event to occur.
Use a 24-hour format, from 0000 to 2359.
The default is 0200.
• Starting on: Select the day of the week for the start of the archive event.
The default is Sunday.
To edit the Autoschedule options, perform the following steps:
1. At the system view Workspace window, select Status – Maintenance.
2. At the Status – Maintenance window, select Archive result data.
3. Select Definition.
4. At the Status – Maintenance Definition window, select Edit.
5. Select Autoschedule.
6. Change 1 or more of the Autoschedule options: Frequency, Time, or Started
on.
7. Select Save.
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4. Select Continue.
You can export test utilization data to a CD or DVD. The system exports it in a
spreadsheet compatible format. The system exports test utilization data in the
following order:
• test name assigned by the manufacturer
• calibrator product code
• number of patient orders
• number of calibrators
• number of controls
• number of total tests used.
The first record of the file contains the system serial number and the date and time
that you performed the export activity.
The system separates each area by tabs and writes the exported data to the file
assay.csv. If the data exceeds the size of the CD or DVD, the system notifies you
and does not export the data. If this happens, you can reduce the time interval
between the starting date and ending date and then export the data again.
NOTE: You must have the appropriate level of security to export data.
1. At the system view Workspace window, select Setup.
2. Select Data Administration.
3. Insert the storage medium into the appropriate drive.
4. At the Setup – Data Administration window, select Test Utilization Data.
NOTE: Exporting data overwrites any existing storage medium.
5. Select Export.
6. At the Setup – Data Administration Options window, select the starting date
and ending date.
• The default for the starting date is 30 days before the current date and
time.
• The default for the ending date is the current date and time.
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ADVIA Centaur XP Operator’s Guide: Configuring the System
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Configuring the System
The ADVIA Centaur XP setup options allow you to customize sample options,
test parameters, and system options for your laboratory. You must have the
appropriate level of security to modify test definitions and setup options. At
the workspace, you can select Setup to access various setup options.
CAUTION
Do not customize options without ensuring that you select the appropriate value
for your laboratory and continuing to evaluate the integrity of that value. Failure
to do so can cause reporting of unexpected results. For example, if you configure
the system to send all results and additional data except results on hold to your
LIS, and the LIS is not configured to accept the information, the LIS may identify
the additional data inappropriately and fail to distinguish between the final results
and the additional data.
Your laboratory is responsible for ensuring that all configurations are correct:
sample options, test parameters, and system options. This is true regardless of
whether your lab personnel set the values,
Siemens Medical Solutions Diagnostics personnel set the values to specifications
your laboratory provides, or Siemens Medical Solutions Diagnostics personnel set
the values to the original default values established at the time of manufacture.
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1 Rack ID
2 Tube positions A, B, C, D, and E
CAUTION
Do not identify samples by rack ID unless absolutely necessary. If it is necessary
to identify a sample by rack ID, be aware that the system ejects the sample to the
exit queue after the sample is aspirated. Reflex or other testing does not occur
after the sample moves to the exit queue. To obtain final results for reflex or other
testing, you must reintroduce the sample rack to the front of the sample entry
queue. Whenever possible, set the system to identify samples by SID.
NOTE: If you specify SID, you can override this selection for a sample by
selecting Schedule by Rack at the Worklist – Schedule window. If you specify
Rack, the system always identifies samples by rack position number and you
cannot select Schedule by SID at the Worklist – Schedule window.
1. At the workspace, select Setup.
2. Select Summary.
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CAUTION
Do not move the tube type selector without verifying the tube type you are using.
Ensure that the tube type selector on each sample rack points to the correct tube
type as defined at this window. If the tube type selector points to an incorrect tube
type, the system can experience problems with sample handling and system errors
can result.
4. At the Setup – Tube Type & Barcode window, use Tube Types 2, 3, and 4 to
select up to 3 types of sample tubes.
The ADVIA Centaur XP system reserves Tube Type 1 for multiple tube types.
Regardless of what you enter here, if the tube-type selector is in position A,
the rack allows multiple tube types. If you use an ADVIA Centaur rack, you
must select a tube type in Tube Type 1.
When you load samples, use the tube type selector on the rack to indicate
the tube type you are loading. Place the tube-type selector in position A for
multiple tube types. Refer to Managing Sample Racks‚ page 2-2.
5. In the LAS Tube Type field, select the tube type you want to use when no tube
type is specified in the LAS.
The default is Auto, meaning it allows multiple tube types.
6. Select Save.
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NOTE: Ensure that you make all of the changes to the Setup – Tube Type &
Barcode window before you reboot the system. If you do not make all of the
changes, you must reboot the system more than 1 time.
7. Reboot the system.
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CAUTION
Use care when entering the SID mapping information. The system uses only the
characters specified in this procedure as patient sample SID codes.
NOTE: You can select a position of the barcode only once.
4. Enter the barcode positions that you want to use starting with the 1 position
field at the bottom of the window.
To map the SID, select up to 13 positions of the barcode to display as the 13
characters of the SID in the worklist. You can select the positions to display in
any order. For example, if you select the number 6 in the third position,
whatever is in the sixth position in the barcode displays in the third position in
the SID.
For positions you do not want to display, select a number greater than the
number of positions in the longest barcode. For example, if you have a
6-character barcode and you want to display the last 3 characters as the SID,
select 4, 5, and 6 for the 1, 2, and 3 position fields, and then select a number
greater than 6 for the 4 through 13 position fields.
If the 6-character barcode is as follows:
ABCDEF
The system reads the following SID code:
DEF
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You can select some symbols to display as characters in the SID, for example
+, -, /, *, #. The symbols display in the specified positions for all SIDs. Do not
select the % symbol because the system does not accept a % symbol in the
SID.
5. Select Save.
NOTE: Ensure that you make all of the changes to the Setup – Tube Type &
Barcode window before you reboot the system. If you do not make all of the
changes at 1 time, you must reboot the system each time you make changes.
6. Reboot the system.
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4. At the Setup – Sample Handling Options window, enter or edit the number
of hours for the interval used by the system to automatically move the results
from the current database to the historical database.
The number of hours must be a factor of 24 (1, 2, 3, 4, 6, 8, 12, or 24).
The time interval starts at midnight.
5. Select Save.
The system moves data to the historical database in the approximate time
interval that you specify, depending on when testing for the samples is
completed.
Reviewing Tests
At the Test – Summary window, you can review the tests defined on your system.
Use this window to edit the test definitions, enable or disable tests, and add or
delete ratio and off-system tests.
1. At the workspace, select Setup.
2. Select Test Definition Summary.
• Select Definition to modify the opened stability date and number of
replicates for each test.
• Select Ranges to define reference ranges for each test.
• Select Display Order to define the order in which tests are displayed and
printed.
Select the appropriate View and Type to display specific information at the
Test – Summary window:
• The View that you select determines the information that the system displays.
For example, if you select All, the system displays all tests.
• The Type that you select determines the order in which the system displays
the tests.
For example, if you select All, the system displays all tests in alphabetical
order.
The following table describes the View and Type combinations you can use to
perform some routine tasks:
Task View Type
display all tests All All
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Enabling a Test
An enabled test is included in the test menu displayed at the ADVIA Centaur XP
windows and the system performs the test if it is scheduled.
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When you enable a test, you do not have to perform the following tasks:
• reenter any Master Curves
• activate an active reagent lot
• recalibrate a valid calibration
NOTE: If your laboratory wants to enable the HBs or Conf test, ensure that you
turn system mechanics off, turn system mechanics on, and then perform a Daily
Cleaning Procedure after enabling the test.
1. At the workspace, select Setup.
2. Select Test Definition Summary.
3. At the Test – Summary window, select the test.
4. Select Enable.
Disabling a Test
NOTE: You cannot disable tests that are part of a combination test, ratio test,
profile, or sIgE universal test.
NOTE: If you schedule the test before disabling it, the disabled test is displayed at
the Worklist – Summary window.
WARNING
Do not disable a test without updating interference information. If you do not
update the interference information, the system may fail to perform the necessary
washes and that could potentially cause erroneous results.
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NOTE: Turning system mechanics off and then turning system mechanics on
ensures that the HBs test is disabled.
6. At the workspace, select System Status.
7. Select Turn System Mechanics Off.
Wait until the system mechanics are off.
8. Select Turn System Mechanics On.
NOTE: The daily cleaning procedure (DCP) provides the system with the
required probe washes, preventing the need to have the disabled
assay-specific wash pack loaded on the system.
9. At the workspace, select Maintenance Status.
10. Select Perform Daily Cleaning.
11. Select Perform.
NOTE: If your laboratory wants to enable a test in the future, ensure that you
turn system mechanics off, turn system mechanics on, and then perform the
DCP after enabling the test. If you are enabling Conf, enable Reagent A and
Reagent B also.
Deleting a Test
Deleting a test permanently removes it from the test menu and permanently
removes any results associated with the test from the database. You can only
delete tests that are defined by your laboratory, such as ratio or off-system tests.
1. At the workspace, select Setup.
2. Select Test Definition Summary.
3. At the Test – Summary window, select a test.
a. Select Test.
The Test–Selection window displays.
b. Select a test.
c. Select Continue to close the Test–Selection window.
4. Complete the appropriate tasks.
If you want to . . . Then select . . .
temporarily stop performing the test, Disable.
add the test to your system test menu, Enable.
remove the test from your system test menu, Delete.
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CAUTION
Do not select the Reset Defaults button at the Test – Definition window. Selecting
this button restores the original options the manufacturer defined and removes any
options that your laboratory defined or modified. This global change can have
unexpected consequences for your laboratory and, in some instances, creates an
unrecoverable error. To change your test definition, refer to Modifying or
Viewing Test Options‚ page 8-9.
6. Change the test name information from the default test name, if necessary:
a. At Display, enter the test name that you want the system to display.
You can enter up to 8 alphanumeric characters for the name the system
displays.
b. At Print, enter the test name that you want to appear on printed reports.
You can enter up to 20 alphanumeric characters for the name the system
displays on printed reports.
7. Select Save.
If you want to restore the original system values for all options at this window,
select Reset Defaults.
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7. View the Control Warning field for the maximum allowable time in hours
between the start and end-of-bracket controls, as defined by
Siemens Medical Solutions Diagnostics.
When the system issues the Control Warning, you must process the
end-of-bracket controls immediately or any retained patient test results are
discarded.
Refer to Using Control-bracketed Tests‚ page 2-58 or consult the index under
Control Bracketing.
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NOTE: If you select Reset Defaults, the system resets all options at the Test –
Ranges window in addition to the reference range options.
7. Reset the ranges to the original values, if necessary:
a. Select Reset Defaults.
b. Select Ranges.
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8. Reset all the parameters and ranges to the original values, if necessary:
a. Select Reset Defaults.
b. Select All.
9. If required, define reflex tests for test results that are outside the reference
ranges.
Refer to Defining Reflex Tests for Reference Ranges‚ page 8-23.
10. Select Save.
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• You can select whether you want to hold samples in the inprocess queue
for repeat tests and the types of samples you want to hold.
If you select . . . Then . . .
both automatic you do not need to interact with the system to repeat a test.
repeat and hold The system holds the racks in the inprocess queue until it
options, completes the results. If the sample requires a repeat test, the
system automatically schedules and performs it. If the inprocess
queue is full, the system can load an additional sample rack after
it ejects a sample rack from the inprocess queue.
automatic repeat you must reload the sample to repeat a test.
options and do not The system automatically schedules repeat tests for results
select hold outside the defined ranges and the tests remain pending until you
options, reload the sample.
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NOTE: If you select Reset Defaults, the system resets all options at the
Test – Ranges window in addition to the check range and linearity options.
10. You can reset the ranges at the Test – Ranges window to the original values:
a. Select Reset Defaults.
b. Select Ranges.
11. You can reset all options and ranges at the Test – Ranges window to the
original values:
a. Select Reset Defaults.
b. Select All.
12. Select Save.
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NOTE: If you select Reset Defaults, the system resets all options at the
Test – Ranges window in addition to the concentration and index calculation
range options.
7. You can reset the options to the original values:
a. Select Reset Defaults.
b. Select All.
8. Select Save.
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NOTE: If you are manually scheduling a repeat test, the field at the Test – Ranges
window does not apply. To manually schedule a repeat test, change the number of
replicates at the Worklist – Reagent Options window, as described in Manually
Scheduling Samples‚ page 2-48.
Use this procedure to define the number of replicates for samples processed in
automatically repeated tests.
1. At the workspace, select Setup.
2. Select Test Definition Summary.
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Select the Hold Sample for Repeat option for each test:
1. At the workspace, select Setup.
2. Select Test Definition Summary.
3. At the Test – Summary window, select a test.
a. Select Test.
The Test–Selection window displays.
b. Select a test.
c. Select Continue to close the Test–Selection window.
4. Select Definition.
5. At the Test – Definition window, select Edit.
6. Select Hold Sample for Repeat.
This holds samples in the inprocess queue for additional testing based on the
repeat criteria you defined at the Test – Ranges window.
7. Select Save.
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NOTE: The Dilution Point, Dilution, or Overdilution Point field may not be
available depending on the displayed test.
NOTE: If you define a Dilution Point, you must also define a Dilution and
Overdilution Point. If a you do not define a Dilution, the system does not have
enough information to automatically dilute a sample. If you do not define an
Overdilution Point, the system does not have enough information to check the
diluted result.
6. In Dilution Point, enter the concentration above which you want the system to
perform an automatic dilution.
If the result is above the concentration limit that you enter in this field, the
system flags the test result and automatically schedules a dilution.
7. In Dilution, select the dilution factor.
8. In Overdilution Point, if it is not defined, enter the concentration below which
you do not want the system to determine a result for the specific dilution.
The Overdilution Point should be equal to the Low Limit of the Linearity
range specified at the Test – Ranges window. If no Low Limit is defined, the
Overdilution Point should be equal to the sensitivity limit of the assay, as
reported in the product instruction in the ADVIA Centaur Assay Manual.
The Overdilution Point provides an indication that the system should have
diluted the sample at a lower level or not at all. The system compares the
uncorrected diluted concentration to the value displayed in Overdilution
Point. If the concentration is below the Overdilution Point, the system does
not report a result for the sample.
For example, if the Overdilution Point for ThCG is 5.0 mIU/mL (IU/L) and
the uncorrected result is 4.5 mIU/mL (IU/L) on a sample diluted 1:200, the
system does not determine a result for the sample because the concentration
is below the specified concentration. The system flags the test result and does
not schedule or repeat the test. To obtain a result, you can process the sample
undiluted or diluted at a lower level, such as 1:5.
NOTE: If you select Reset Defaults, the system resets all options at the Test –
Ranges window in addition to the automatic dilution options.
9. Reset all options to the original values, if necessary:
a. Select Reset Defaults.
b. Select All.
10. Select Save.
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When you request a ratio test, the system automatically schedules all tests defined
as part of the ratio. If you request a ratio test and also request a test that is part of
the ratio test, the system performs the test twice. One result is used in the
calculation of the ratio test and the other result is reported independently.
NOTE: When you edit an element of a ratio test, the system automatically
recalculates the ratio test result and reports the result with an Edited flag.
1. At the workspace, select Setup.
2. Select Test Definition Summary.
3. At the Test – Summary window, define or edit a ratio test:
If you want to . . . Then . . .
define a ratio test, a. Select Add Ratio.
b. Enter the test.
edit a ratio test, a. Select a ratio test.
b. Select Definition.
c. At the Test – Ratio Definition window, select Edit.
4. At the Test – Ratio Definition window, enter the parameters:
If you want to . . . Then . . .
enter the LIS code for a test, enter the LIS code.
review the result before the system select Hold.
reports the result,
enter the units, select a unit of measure.
enter the number of decimal places, select the number of significant digits in
Decimal.
enter a reference range, enter the low limit and the high limit.
NOTE: Use the formula displayed at the Test – Ratio Definition window to
define each ratio test.
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5. Select Save.
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Defining Alarms
You can define alarms for Message Boxes, and Warning or Failure conditions.
Refer to Monitoring Status‚ page 2-9.
1. At the workspace, select Setup.
2. Select Summary.
3. At the Setup – Summary window, select Alarms.
4. At the Setup – Alarms window, select Message Box, Warning, or Failure.
5. Select Alarm On or Alarm Off.
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4. At the Setup – Additional Options window, enter the time correction for the
system clock:
If you are entering a . . . Then . . .
new time correction that was not enter the number of seconds with a plus sign to
used in the previous setting for this increase the time or a minus sign to decrease the
window, time (example: +300 to increase the time 5
minutes or –300 to decrease the time
5 minutes).
time correction that was used in the a. Enter 0 (zero) in the Time Correction
previous setting for this window field.
(example: you entered a time b. Select Save.
correction of +300 seconds, the
system adjusted the time by this c. Enter the number of seconds with a plus
amount, and you want to adjust the sign to increase the time or a minus sign
time by +300 seconds again), to decrease the time (example: +300 to
increase the time 5 minutes or –300 to
decrease the time 5 minutes).
5. Select Save.
The system gradually adjusts the time on the system clock according to the
number of seconds that you entered in the Time Correction field. It can take
3 hours or longer for a correction of 10 minutes.
NOTE: Do not reboot after entering a time correction until the system
completes the correction. Rebooting stops the time correction process.
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2. Select Summary.
3. At the Setup – Summary window, select Print Options.
4. At the Setup – Print Options window, select the appropriate printer and
paper size.
5. You can print an automatic runtime results report, which is a report of all
sample results as the results are being generated:
a. Select Automatic Runtime Results Report.
b. Enter the interval in hours at which you want page numbering to reset to 1
in Reset Page Numbering.
c. Enter the number of full pages of results to print at 1 time in Page
Interval.
6. If applicable, you can enter the customer number assigned to you by QC
Online.
7. You can enter the laboratory information you want to appear on printed
reports
• In Laboratory Name, enter up to 30 characters of text for your laboratory
name that you want to print on reports.
• In Report Header, enter up to 80 characters and up to 6 lines of text that
you want to print in the report header.
8. Select Save.
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3. Select Setup.
4. In the Target Backup/Archive Device drop-down list, select the device you
want to use for backing up data, restoring data, or storing your archive.
5. If you are using the RealTime Solutions service, select Access to Server.
You must select this option to enable the other backup/archive options.
6. Select each option that your laboratory uses:
If you want to . . . Then select . . .
send your results data to the RealTime Solutions server, Result Data to Server.
send your backup data to the RealTime Solutions server, Backup Data to Server.
send your logfiles to the RealTime Solutions server, Logfiles to Server.
7. In Time of Day, enter the time of day for the system to send files to the
RealTime Solutions service.
Specify time in a 4-digit, 24-hour format HHMM. For example: 0130 is 1:30
a.m. and 1330 is 1:30 p.m.
8. Select Save.
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Appendix A: Safety Instructions
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A-2 ADVIA Centaur XP Operator’s Guide: Safety Instructions
References
1. Centers for Disease Control. 1988. Update: Universal precautions for
prevention of transmission of human immunodeficiency virus, hepatitis B
virus and other bloodborne pathogens in healthcare settings. MMWR,
37:377–382, 387, 388.
2. Clinical and Laboratory Standards Institute (formerly NCCLS). Protection of
Laboratory Workers from Occupationally Acquired Infections; Approved
Guideline - Third Edition. CLSI Document M29-A3.[ISBN 1-56238-567-4].
Clinical and Laboratory Standards Institute, 940 West Valley Road, Suite
1400, Wayne, Pennsylvania 19087-1898 USA, 2005).
3. Federal Occupational Safety and Health Administration. Bloodborne
Pathogens Standard. 29 CFR 1910. 1030.
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ADVIA Centaur XP Operator’s Guide: Safety Instructions A-3
laserclass1
.
Some field service procedures require the removal of the protective housings that
prevent human access to the laser radiation. The removal of the protective
housings may change the classification of the system to CDRH Class 2 and
EN60825-1 Class 2. All field service procedures must be followed precisely. Only
Siemens-trained field service personnel should perform procedures related to laser
assemblies.
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A-4 ADVIA Centaur XP Operator’s Guide: Safety Instructions
LASER WARNING
Never look directly at the laser beam or at its reflection from a shiny surface.
Looking directly at the laser beam or its reflection could cause damage to the
eyes. Only trained field service personnel should perform procedures related to
laser assemblies. Refer to Appendix A, Protecting Yourself from Barcode Scanner
Lasers, for more information.
The specifications for the laser optical assemblies in the ADVIA Centaur XP
ancillary reagent, primary reagent, and sample barcode scanners are summarized
in the following table:
Characteristic Specification
Maximum Power Output 1.0 mW
Wavelength 670 nm
Pulse Duration Continuous Wave (cw)
Units of Beam Divergence 0.7 mr
The locations of the reagent and sample barcode scanners and their associated
laser safety labels are shown below. The laser apertures for the sample and
ancillary reagent barcode scanners are directed into the system away from the
operator.
During normal operation, reflections from the sample barcode scanner laser beam
may be visible to persons in the immediate area, but no laser safety hazard is
associated with this exposure. The laser aperture for the primary reagent barcode
scanner is directed toward the barcode labels at the end of the reagent packs inside
the primary reagent compartment.
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During normal operation, the barcode scanner does not scan the reagent packs
until the primary reagent compartment door is closed. When the primary reagent
compartment door is closed, no operator exposure to the laser exists.
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This table contains a summary of the specifications for the laser optical assembly
in the ADVIA Centaur XP hand-held barcode scanner:
Characteristic Specification
Maximum Power Output 1.0 mW
Wavelength 650 nm
Pulse Duration 7.4 ms
Units of Beam Divergence 2mr
The laser aperture and laser safety label for the hand-held barcode scanner are on
the other side of the barcode scanner window:
Hand-Held3 1
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Appendix B: Service, Ordering, and Warranty
Warranty Period
The limited warranty period generally commences upon installation of the
original instrument at the customer’s location and extends for a period of 1 year
thereafter, unless otherwise specifically agreed to by and between
Siemens Medical Solutions Diagnostics (or its authorized distributors) and
customer in a writing signed by duly authorized representatives of both parties
(sales representatives are generally not authorized representatives of Siemens for
these purposes).
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Replacement of Parts
In performing service, Siemens Medical Solutions Diagnostics or its authorized
distributors provide appropriate parts to repair the instrument, or arranges for the
exchange of the instrument or affected parts, at no charge with the exception of
certain parts or subassemblies that are considered Customer Maintenance Items.
Customer Maintenance Items include, but are not limited to, the following items:
lamps, electrodes or sensors (which are covered by a separate warranty), reagents,
calibrators, controls, paper, and pens. Consult the appropriate system operator’s
manuals for a complete list of Customer Maintenance Items for any specific
model of instrument.
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B-6 ADVIA Centaur XP Operator’s Guide: Service, Ordering, and Warranty
3. Select Print.
4. Select Print Workspace.
Contacts
This section provides the following information:
• the address of the Siemens authorized representative, which is the Siemens
contact within the European community
• the Siemens addresses for obtaining service and technical information and for
ordering supplies
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Addresses
For technical assistance contact your local technical support provider. For
customer service or additional information contact your local technical support
distributor.
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Appendix C: Reagents and System Fluids
Handling Reagents
This information describes handling requirements for the following
ADVIA Centaur XP reagents.
WARNING
Use care when using diagnostic tools or performing troubleshooting. System
components move and can cause injury. Only Siemens-trained personnel
should perform troubleshooting
Unpierced
For all other assays, use this procedure if the primary reagent pack is unpierced.
1. With the film side up, hold the reagent pack loosely at the ends with the
thumb and index or middle finger of each hand.
2. Carefully raise 1 end of the pack 90× so that the pack is in a vertical position.
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3. Carefully raise the other end of the pack 90× so that the pack is again in a
vertical position. Carefully return the pack to a horizontal position.
4. Repeat steps 2 and 3 a minimum of twenty times or until:
• the pellet is broken up and no longer visible on the bottom of the pack
• no large aggregates are visible floating inside the pack.
5. Adjust the mixing speed to minimize foaming.
6. Mix 5 to 10 times more to ensure complete mixing.
7. Load the unpierced primary reagent pack onto the system.
When testing is complete you may remove the reagents from the system or leave
them on the system. If you remove the reagents, cover the pierced film area of the
primary reagent pack with self-sealing laboratory film and place the pack in the
reagent storage tray at 2 to 8×C. Store pierced primary reagent packs upright in
the storage tray to minimize spillage. Follow the pierced primary reagent pack
procedure before placing the reagent pack back on the system. Reagents left on a
system with the power on are automatically mixed by the system and do not
require further mixing.
Pierced
Use this procedure if the primary reagent pack is pierced.
1. Press gently on the self-sealing laboratory film that covers the pierced film
area while mixing.
This prevents reagent leakage.
2. With the film side up, hold the reagent pack loosely at the ends with the
thumb and middle finger of each hand.
3. From a horizontal position, carefully raise 1 end of the pack 45× and carefully
return the pack to a horizontal position.
4. Carefully raise the other end of the pack 45× and carefully return the pack to a
horizontal position.
5. Repeat steps 3 and 4 a minimum of twenty times or until 1 of the following
actions is true:
• the pellet is broken up and no longer visible on the bottom of the pack.
• no large aggregates are visible floating inside the pack.
6. Adjust the mixing speed to minimize foaming.
7. Mix 5 to 10 times more to ensure complete mixing.
8. Remove the self-sealing laboratory film and load the pierced reagent pack
onto the system.
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When testing is complete you may remove the reagents from the system or leave
them on the system. If you remove the reagents, cover the pierced film area of the
primary reagent pack with self-sealing laboratory film and place the pack in the
reagent storage tray at 2 to 8×C. Store pierced primary reagent packs upright in
the storage tray to minimize spillage. Follow the pierced primary reagent pack
procedure before placing the reagent pack back on the system. Reagents left on a
system with the power on are automatically mixed by the system and do not
require further mixing.
NOTE: If reagent mixing is turned off for longer than 2 hours but reagent
refrigeration remains on, mix all primary reagent packs following the unpierced/
pierced primary reagent pack procedures and place the reagent packs back on the
system. Verify reagent performance based on acceptable quality control results or
by criteria established for your laboratory.
If both reagent mixing and reagent refrigeration are turned off for longer than 2
hours, put the primary reagent packs in the reagent storage tray at 2 to 8×C. Mix
all primary reagent packs following the unpierced/pierced primary reagent pack
procedures before placing the reagent packs back on the system. Verify reagent
performance based on acceptable quality control results or by criteria established
for your laboratory.
NOTE: The onboard stability of a primary reagent pack is calculated from the
time that the system reads the barcode after the reagent pack is placed on the
system for the first time. If a pack is removed from the system, it is recommended
that you reload the pack onto the same system. If you load the reagent pack onto a
different system, you must track onboard stability and you must adjust reagent test
inventory to account for tests previously removed from the pierced reagent pack
while on the other system.
Unpierced
Gently invert ancillary reagent packs several times before loading them into the
ancillary queue. It is important to minimize foaming. Do not shake the ancillary
reagent pack.
When testing is complete you may remove the reagents from the system or leave
them on the system. If you remove the reagents, cover the pierced film area of the
ancillary reagent pack with self-sealing laboratory film and store the pack at 2 to
8×C. Store pierced ancillary reagent packs upright to minimize spillage. Follow
the pierced ancillary reagent pack procedure before placing the reagent pack back
on the system.
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Pierced
Use this procedure if the ancillary reagent pack is pierced.
1. Press gently on the self-sealing laboratory film that covers the pierced film
area while mixing.
This prevents reagent leakage.
2. Gently invert the pack several times.
3. Remove the self-sealing laboratory film and load the pierced ancillary reagent
pack onto the system.
When testing is complete you may remove the reagents from the system or leave
them on the system. If you remove the reagents, cover the pierced film area of the
ancillary reagent pack with self-sealing laboratory film and store the pack at 2 to
8×C. Store pierced ancillary reagent packs upright to minimize spillage. Follow
the pierced ancillary reagent pack procedure before placing the reagent pack back
on the system.
Do not add reagent to ancillary reagent packs. Each ancillary reagent pack
contains enough reagent to process a predetermined number of tests. Each time
the system accesses an ancillary reagent pack, reagent volume inventory tracks
the amount of reagent used until the pack is empty. The system does not recognize
additional fluid placed in an ancillary reagent pack.
NOTE: If reagent refrigeration is turned off for longer than 2 hours, store the
ancillary reagent packs at 2 to 8×C. Mix all ancillary reagent packs following the
unpierced/pierced ancillary reagent pack procedures before placing the reagent
packs back on the system. Verify reagent performance based on acceptable quality
control results or by criteria established for your laboratory.
NOTE: The onboard stability of an ancillary reagent pack is calculated from the
time that the pack is first pierced. If a pierced pack is removed from the system, it
is recommended that you reload the pack onto the same system. If you load the
reagent pack onto a different system, you must track onboard stability and you
must adjust reagent volume inventory to account for any reagent previously
removed from the pierced reagent pack while on the other system.
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Good laboratory practices suggest that you establish a protocol that supports the
manufacturer’s requirement for the instrument to:
• ensure optimum performance of automated laboratory instruments
• eliminate water quality as a source of problems when troubleshooting
• help you to meet requirements for state and federal laboratory certification
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Purifying Water
As with all diagnostic testing procedures, good laboratory practices suggest that
you establish a protocol that supports the manufacturer’s requirements for
selecting the appropriate type of reagent water. You can then produce reagent
water in your laboratory by setting up and maintaining a water purification system
which uses the purification methods described in the following table.
The following table describes some of the typical laboratory water purification
methods.
Method Description
Distillation Changes water from liquid to vapor and leaves behind
impurities such as particulates and bacteria
Deionization Uses synthetic resins to remove ionized impurities by ion
exchange
Reverse Osmosis Forces water under pressure through a semipermeable
membrane to remove dissolved solids and organic impurities
Adsorption Uses activated carbon, clays, silicates, or metal oxides to
remove organic impurities
Filtration Forces water through a semipermeable membrane to remove
insoluble matter, emulsified solids, pyrogens, and
microorganisms
The quality of the reagent water you produce depends on the quality of the water
you start with (source water), and the performance of your water purification
system.
To produce the type of water you require, you may need a purification system that
uses a combination of methods. For example, if you want to produce Type I water,
you need a system that uses adsorption to remove organic impurities, deionization
to remove ionized impurities, and filtration to remove particulates.
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1 Pump
2 Source Water
3 Check Valve
4 Flow Controller
5 Resistivity Indicator
6 Valve
7 Filter
8 Type I Water Output
9 Ion Exchange
10 Carbon
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References
1. Clinical and Laboratory Standards Institute (formerly NCCLS). Preparation
and Testing of Reagent Water in the Clinical Laboratory; Approved
Guideline- Third Edition. NCCLS document C3-A3 (ISBN 1-56238-336-1).
Clinical and Laboratory Standards Institute, 940 West Valley Road, Suite
1400, Wayne, Pennsylvania 19087-1898 USA, 1997.
2. Tietz, Norbert W. Fundamentals of clinical chemistry, 3rd ed. Philadelphia:
W.B. Saunders Company; 1987. 1010 p.
3. Kaplan, Lawrence A. Pesce, Amadeo J. Clinical Chemistry; theory, analysis,
and correlation, 2nd edition. St. Louis: C.V. Mosby Company; 1989. 1212 p.
4. College of American Pathologists, Laboratory General Inspection Checklist
Section 1, Quality of water, p. 8. Northfield, IL. 1989.
CAUTION
Do not enter values in SI units when you enter Master Curve data or calibrator
assigned values. Using SI units may cause erroneous results.
NOTE: Do not enter an SI conversion factor for assays that do not list a factor.
The system does not accept an SI conversion factor for these tests.
If you report results in SI units, enter the appropriate units and conversion factor
for each test at the Test – Result Calculation window.
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Conversion
Assay Common Units SI Units Factor
AFP ng/mL IU/mL 0.83
Allergy Screen (ALSCRN) mIU/mL mIU/mL 1
BNP pg/mL pmol/L 0.289
BR (CA 15-3®) U/mL U/mL 1
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Conversion
Assay Common Units SI Units Factor
HIV 1/0/2 Enhanced (eHIV) Index none 1
Total IgE (tIgE) IU/mL IU/mL 1
Intact PTH (iPTH) pg/mL pmol/L 0.106
Insulin (IRI) mU/L mU/L 1
LH mIU/mL IU/L 1
Myoglobin (MYO) ng/mL µg/L 1
Phenobarbital (PHNB) µg/mL µmol/L 4.31
Phenytoin (PHTN) µg/mL µmol/L 3.96
Progesterone (PRGE) ng/mL nmol/L 3.18
Prolactin (PRL) ng/mL µIU/mL 21.2
PSA ng/mL µg/L 1
cPSA ng/mL µg/L 1
Rubella G (Rub G) IU/mL IU/mL IU/mL
Rubella M (Rub M) Index none 1
Specific IgE (sIgE) kU/L kU/L 1
T3 ng/mL nmol/L 1.54
T4 µg/dL nmol/L 12.9
anti-TG (aTG) U/mL U/mL 1
anti-TPO (aTPO) U/mL U/mL 1
Testosterone (TSTO) ng/dL nmol/L 0.0347
Theophylline 2 (THEO-2) µg/mL µmol/L 5.55
TnI-Ultra™ ng/mL µg/L 1
Tobramycin (TOBR) µg/mL µmol/L 2.14
Total hCG (ThCG) mIU/mL IU/L 1
Toxoplasma G (Toxo G) IU/mL IU/mL 1
Toxoplasma M (Toxo M) Index none 1
TSH µIU/mL mIU/L 1
TSH-3 µIU/mL mIU/L 1
TUp Index none 1
Valproic Acid µg/mL µmol/L 6.93
Vancomycin µg/mL µmol/L 0.69
VB12 pg/mL pmol/L 0.738
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For example, for a TSH-3 scheduled in a small transfer tube, the values
are as follows:
200 µL + 20 µL + 100 µL = 320 µL
• For multiple tests scheduled for a sample, use the following formula:
Sum of (Sample Volume + Tip Prime Volume) for all repli-
cates for all tests + Unusable Volume = MRV
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System Fluids
The fluids described in the table below are for In Vitro Diagnostic Use only. Use
the fluids until the expiration date stated on the bottle labels. Use Acid and Base
reagents until the expiration date stated on the bottle label or until 28 consecutive
days after loading the reagent on the system.
Dispose of Acid and Base reagents and cleaning solution using approved disposal
techniques for hazardous corrosive liquid wastes. Dispose of Acid and Base
reagents in accordance with local, state, and federal regulations. Refer to the
Material Safety Data Sheets for each material for additional information.
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WARNING
Do not allow contact of Acid and Base reagents with skin and eyes. Acid and Base
reagents are irritating to the skin. Wear suitable eye, face, and skin protection,
which include wearing gloves, protective eye shield, and laboratory coat. In case
of contact with the eyes, rinse the eyes immediately with plenty of water and seek
medical advice. In case of accident or if you feel unwell, seek medical advice. If
possible, show the bottle label and the Material Safety Data Sheet. In case of
accidental ingestion of reagent, call a physician immediately.
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Appendix D: Supplies
The following is a list of supplies with catalog numbers and REF to assist in the
ordering process. The catalog numbers in parentheses are included in the list for
reference only.
Description REF Catalog Number
Barcode Labels, Sample Identification 05296798 (23531)
Brush, Small Maintenance (10) 4934626 (27081)
Cleaning Solution Concentrate 09908593 (112748)
Cuvettes (package of 3000) 08044064 (078-K138-01)
Cuvette Waste Bin 06412376 (572615)
Filter, Air Intake 03918457 (572165)
Filter, Water 02757433 (26970)
Labels, Sample Rack
range 001 – 100 9391302 (078-0333-01)
range 101 – 200 3828105 (078-0334-01)
range 201 – 300 8579502 (078-0335-01)
range 301 – 400 1160808 (078-0336-01)
range 401 – 500 2115415 (078-0337-01)
Organizer, Master Curve Cards, 03364923 (672003)
Barcode Labels, and Calibrator Cards
Pointing Device Convertor 06097004 (115625)
Printer Paper (8.5 x 11” paper, 1-part) 02884702 (20764)
Printer Paper (8.5 x 11” paper, 3-part) 00016339 (751046)
Probe, Ancillary and Reagent 04042237 (571233)
Probe, Ungrounded Aspirate 03040281 (571843)
Probe, Grounded Aspirate 02873484 (125669)
Reagent Storage Tray 06104191 (119147)
Reagents, Acid and Base (5000 tests 03852677 (112219)
per bottle)
Sample Cups (bag of 1500) 03832935 (078-K137-01)
Sample Cup Caps (package of 1000) 07286900 (672010)
Sample Racks, unlabeled (box of 60) 7609246 (078-K225-01)
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D-2 ADVIA Centaur XP Operator’s Guide: Supplies
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Appendix E: Specifications
This section summarizes the design specifications for the ADVIA Centaur XP
system.
Safety Certifications
Refer to the DECLARATION OF CONFORMITY shipped with the
ADVIA Centaur XP system.
Dimensions
Depth 104.2 cm (41.0 in)
Height 130.9 cm (51.5 in)
Width 194.3 cm (76.5 in)
Weight 559 kg (1,232 lbs); system has casters
Environmental Specifications
Ambient Operating Temperature 18.0 to 30.0 ºC
Relative Humidity 20% to 80%, non-condensing,
actively controlled
Storage Temperature 0.0 to 50.0 ºC
Indoor Use Only
Altitude up to 2000 meters
Ventilation 10,000 BTU/hour
IEC 1010-1 Installation Category II
IEC 1010-1 Equipment Classification Class I
IEC 1010-1 Pollution Degree 2
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E-2 ADVIA Centaur XP Operator’s Guide: Specifications
Electrical Requirements
Electrical Rating 220 to 240VAC ± 10% ~8A
Power Requirements 50/60Hz
Tube Types
This section lists the primary sample tubes, sample transfer cups, transfer tubes,
and microsample containers you can use on your system.You can specify up to 3
tube types at the Setup – Tube Type & Barcode window. Refer to Specifying
Sample Tube Types‚ page 8-5.
The tube type fields at the Setup – Tube Type & Barcode window list the tubes
you can use on your system. The + symbol before the tube type indicates that you
need to place it into a holder before loading it into the sample rack.
Ensure that translucent plastic sample tubes have barcode labels or a printed label
facing the barcode reader when you load the tubes onto the system. The system
might not detect a translucent tube with no label or a white label with very little
printing.
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E-4 ADVIA Centaur XP Operator’s Guide: Specifications
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Appendix F: System Symbols
This section describes the symbols that can display on the exterior of the
ADVIA Centaur XP system or on the system packaging. The symbols on the
system identify the location of certain components and, where necessary, display
warnings for proper operation. The symbols on the system packaging provide you
with other important information.
For information about symbols that appear in the ADVIA Centaur XP system
workspace and windows, refer to Understanding Worklist Symbols‚ page 2-41.
Symbol Description
This symbol is used for both Warnings and Cautions.
• A Warning indicates the risk of personal injury or loss of life if
operating procedures and practices are not correctly followed.
• A Caution indicates the possibility of loss of data or damage to or
destruction of equipment if operating procedures and practices
are not strictly observed.
This symbol alerts you to a biohazard.
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F-2 ADVIA Centaur XP Operator’s Guide: System Symbols
Symbol Description
This symbol indicates that the main power supply is off.
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ADVIA Centaur XP Operator’s Guide: System Symbols F-3
Symbol Description
This symbol indicates the Stat entry.
This symbol identifies the system fluid bottle for Acid reagent.
This symbol identifies the system fluid bottle for Base reagent.
This symbol identifies the system fluid bottle for wash 1 solution.
This symbol identifies the system fluid bottle for wash 3 solution.
This symbol identifies the system fluid bottle for cleaning solution.
This symbol identifies the lid that provides access to the liquid waste
bottle.
This symbol identifies the door that provides access to the sample tip
tray waste.
This symbol identifies the door that provides access to the cuvette
waste bin.
This symbol identifies the door that provides access to the sample tip
waste bin.
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F-4 ADVIA Centaur XP Operator’s Guide: System Symbols
Symbol Description
This symbol indicates that you should protect the product from strong
magnetic fields.
This symbol indicates that you should consult instructions for use.
This symbol indicates that the product is fragile and you need to
handle it with care.
This symbol indicates that you should keep the product dry.
This symbol indicates the number used for ordering a part or product.
This symbol indicates the serial number of a part or product.
This symbol indicates the revision letter of a part or product.
This symbol indicates the name and location of the product
manufacturer.
This symbol indicates that the package is printed with soy ink.
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ADVIA Centaur XP Operator’s Guide: System Symbols F-5
Symbol Description
This symbol indicates that the product complies with the applicable
directives of the European Union.
This symbol indicates that the product is CSA approved for safety
(Canada).
The following symbols can also display on the exterior of the ADVIA Centaur XP
system or 1 of its components.
Symbol Description
This symbol indicates that the product is a Class 1
laser product, with no laser exposure during
normal operation.
This symbol indicates that the product is a Class 2
laser product, with potential exposure to a laser
beam.
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F-6 ADVIA Centaur XP Operator’s Guide: System Symbols
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Appendix G: Installation and Relocation
CAUTION
Do not move or install your ADVIA Centaur XP system. Unauthorized movement
or installation can damage your system and void your warranty and/or service
contract. Unauthorized movement can also affect your instrument calibrations. A
Siemens technical support provider should install or relocate your
ADVIA Centaur XP system.
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G-2 ADVIA Centaur XP Operator’s Guide: Installation and Relocation
1 Touch-screen monitor
2 Keyboard
3 Mouse
4 Applications module
5 User interface module
6 Input/Output panel
7 Handheld barcode scanner
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ADVIA Centaur XP Operator’s Guide: Installation and Relocation G-5
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G-6 ADVIA Centaur XP Operator’s Guide: Installation and Relocation
Figure G-6 ADVIA Centaur XP System – Back View I/O Panel Access
Cover
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ADVIA Centaur XP Operator’s Guide: Installation and Relocation G-7
2. Lift the cable panel away and put it safely to the side to expose the back of the
2 modules.
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ADVIA Centaur XP Operator’s Guide: Installation and Relocation G-8
CAUTION
Do not slide the user interface module too far. You can damage the user infterface
module if you slide it off the support rails. Be careful with the cables and the
user interface module when sliding the user interface module.
1. At the back of the system, locate and loosen the anchor screw or screws for
the selected module.
The Applcations module has 1 anchor screw and the User Interface module
has 2 anchor screws. These are captive screws that remain in place when you
unscrew them from the module.
NOTE: The figure displays the back of the system without the cable panel. In
most cases, it is not necessary to remove the cable panel.
1 Applications module
2 Applications module support rail
3 Applications module anchor screw
4 User Interface module
5 User Interface module support rails
6 User Interface module anchor
2. Slide the module toward the front of the system until the I/O panel is
accessible.
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Appendix H: Glossary
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ADVIA Centaur XP Operator’s Guide: Glossary H-3
baud rate Speed of data transmission in bits per seconds (bps) between
the system and a remote device.
bidirectional interface Interface configuration allowing information to be
transferred to and from another device.
buffer (chemistry) Solution composed of a weak acid or base and its salt that is
used for controlling ionic strength. Buffers maintain the pH
of a solution.
buffer (electronic) Data storage used to compensate for differences in
information flow rate or in the time that events occur when
data is transmitted between devices.
calibration The use of 2 samples of known concentrations that you
process on your system. The calibration data is then used to
normalize your system against a stored master curve. The
system calculates results from the normalized curve.
calibration curve Data that normalizes the system against stored Master Curve
data for the tested analyte. The calibration data compensates
for current running conditions and instrument variability.
The system calculates the required sample dose from the
normalized curve.
calibration data Details of the calibration including the SID, concentration,
RLU, CV, and flags for the low and high calibrators.
calibration interval Number of days or hours between required calibrations.
calibrator Solution that contains a known concentration or a
known reactivity of 1 or more analytes and that provides a
reference for converting measured signals
into concentrations. Calibrators are analyzed with reagents
to obtain calibration data points. See calibration data.
Calibrator Assigned Card that contains the calibrator information for a
Values card specific lot of calibrators. The card contains the lot number
and expiration date, and the high and low calibrator values
for each analyte contained in the calibrator. This information
is on the card in text and barcodes for you to enter in the
system.
cancel Function used to ignore changes made at a window.
catalog number A unique number associated with each Siemens product.
The number is used when ordering the product.
CD-ROM Compact disc read-only memory. An electronic storage
medium on which you can store data but does not allow you
to edit or write over the data
CD-RW Compact disc rewritable. An electronic storage medium that
allows you to overwrite the current files on the CD.
central processing unit Main printed circuit board of a computing system that
interprets instructions and directs the system operations.
Abbreviation: CPU.
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ADVIA Centaur XP Operator’s Guide: Glossary H-5
context-sensitive online Information that is about the operator’s current task and that
information is accessible without interrupting the current task.
continuous level sensing Measurement of a wide range of resolvable fluid levels. For
example, sample levels can be measured at any volume.
continuous operation Operation allowing the system to process samples
without interruptions to add or remove samples, empty
waste containers, or replenish supplies.
control Quality control material used to determine if the system
is reporting valid results.
control-bracketed test A test that requires you to begin and end a group of patient
samples with a set of controls. The system does not process
samples until it establishes the acceptability of the results of
the start-of-bracket controls. The system does not report
sample results until it establishes the acceptability of the
results of the end-of-bracket controls is established.
control bracket reagent A setting that allows you to ensure that the system has
reserve enough reagent to complete the end-of-bracket controls of a
control-bracketed test that is in process.
control reminder A system reminder that you should process end-of-bracket
controls soon to prevent the system from discarding retained
patient test results. The control reminder applies only to
control-bracketed tests. The timing for the control reminder
is configurable at the Test – Definition window.
control warning A system warning that you must process the end-of-bracket
controls as soon as possible or the system discards retained
patient test results. The control warning applies only to
control-bracketed tests. The timing for the control warning is
established by the manufacturer.
control value range Range of acceptable results established by the quality
control material manufacturer. The control value range
is provided in the assay manual.
corrective action Activity performed by the operator to solve a problem.
cursor A bar at the window that indicates where you can enter data.
cut-off point Medical decision point for a qualitative assay. Results above
the cut-off point are reported with a different qualitative
interpretation than those below the cut-off point.
cuvette Plastic, disposable container that holds the reaction mixture
on the system. The chemiluminescent reaction occurs inside
the cuvette.
cycle count Each time the sample probe picks up a sample tip, the cycle
count increments by 1.
dark count Electronic background signal of the photomultiplier tube
(PMT) in the absence of light.
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H-8 ADVIA Centaur XP Operator’s Guide: Glossary
incubation ring Area where cuvettes are incubated at 37°C to prepare the
sample and reagent for the chemiluminescent reaction. The
incubation ring also positions the cuvettes for sample and
reagent dispenses and magnetic particle washes and
resuspensions. Also called ring.
index Qualitative result that represents the relative concentration
of an analyte based on a cutoff.
inprocess Status of a test when the sample is in the inprocess queue
and is detected by the system or the sample is in progress.
Status of the system when it is processing samples.
intercept Factor used with slope to correlate the assay results with
another method. You obtain the intercept and the slope from
a regression equation based on method comparison studies.
key operator Designated operator available to Siemens representatives to
describe instrument malfunctions by telephone and/or to
perform simple adjustments and corrections as requested.
keyboard System component you use to type information or to
respond to a system prompt.
label Substance coupled to an antigen or antibody that
provides the measurement signal for an immunoassay.
laboratory information Laboratory computer system that can be interfaced to
system the system. Abbreviation: LIS.
light count Measured electronic signal of the photomultiplier tube
(PMT) during the chemiluminescent reaction.
light emitting diode Electronic device made from semiconductor materials
that emits light when current flows through it. Abbreviation:
LED.
link Method of directly accessing information or data. A link in
the user interface transfers information to a related window
based on a selection. Required data must be present for the
information to transfer. In the online information system, a
link is a quick way to access more information about a topic.
LIS Code Alphanumeric code that identifies a test, profile, or
calibrator to your hospital or laboratory computer.
Lite Reagent Assay reagent containing antibody or antigen labeled
with acridinium ester in buffer.
luminometer Subassembly and component of the system where the
chemiluminescent reaction occurs and is measured.
lyophilized material A liquid material that is freeze-dried to a powder for long
term stability. Reconstitute lyophilized material with water
or other liquid before use.
manual dilution A dilution of the sample made before placing the sample on
the system.
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ADVIA Centaur XP Operator’s Guide: Glossary H-9
Master Curve Multi-point curve that is generated for each lot of Solid
Phase and Lite Reagent for a particular test. The Master
Curve is the reference curve used to convert a measured
signal into sample concentration. Calibration data
normalizes the system against stored Master Curve data for
the tested analyte.
minimum detectable Smallest reagent or sample volume detectable by the system.
volume Also called base volume.
modem Device that converts the digital signals generated by the
computer’s serial port to the modulated analog signals
required for transmission over a telephone line and
transforms incoming analog signals to their digital
equivalent.
monitor Device that produces an on-screen display.
monoclonal antibody Antibody produced through the fusion of a myeloma
cell and a B lymphocyte. Antibodies produced by a
single fusion event have identical structure, affinity,
and specificity for a given antigen.
nonreactive Nonreactive result. A result below the cut-off limit for a
reactive result.
normal range See reference range.
numeric keys Keys that are labeled with numbers and mathematical
symbols and are on the right side of the keyboard.
observed limits Range calculated by your system for each test after the
fourth valid calibration of a reagent and calibrator lot
combination. The system uses data from additional valid
calibrations of the reagent and calibrator lot combination to
continually recalculate this range.
off-system test Test that is not run on the system but whose results are
entered in the result database and printed on a patient report.
You can use off-system test results in ratio tests.
optimize placement Function that indicates where to place reagent packs so that
reagent probes can most efficiently operate.
Pack ID Identification code contained in the barcode on a reagent
pack that the system recognizes as unique to that pack.
paramagnetic particles Magnetic particles used in the Solid Phase reagent.
Abbreviation: PMP.
patient identification Unique code that identifies the patient from whom a sample
code is obtained. Abbreviation: PID.
pending samples Samples for which tests are scheduled or inprocess, but not
complete.
pending tests Tests that are scheduled or inprocess, but not complete.
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H-12 ADVIA Centaur XP Operator’s Guide: Glossary
sample probe Component that picks up a sample tip and then aspirates
a sample and dispenses it into a cuvette.
sandwich assay Antibody-antigen reaction in which the antigen of unknown
concentration in a sample is sandwiched between antibody
bound to solid phase and antibody labeled with acridinium
ester. The measurable signal produced by the reaction is
directly proportional to the concentration of antigen in the
sample.
save Function used to add an object to the database or to include
updates to an object in the database.
screen Component of the monitor that displays windows, programs,
menus, data, prompts, messages, and other operating
information.
search Function used to locate an item in a list.
sign in To enter a password or code to access the system or an area
of the system.
sign out To indicate that you are finished working in the system or an
area of the system.
SI units International system for units of measure.
SID Sample identification.
slope Factor used along with intercept to correlate the assay results
with results from another method. You obtain the slope and
the intercept from a regression equation based on method
comparison studies.
software Computer instructions that generate and carry out
commands to control system operation.
Solid Phase An assay reagent consisting of paramagnetic particles
covalently bound to antigen or antibody suspended
in a buffer.
sort Function used to change the order of items in a list.
For example, you can sort the worklist by patient name or by
SID.
stability Degree of a reagent’s resistance to chemical change
or degradation.
startup The events that occur when the system uses the software to
initialize the hardware.
status Information about the current operating condition of
the system.
stop bits Number of data bits that maintain synchronization between
the system and a remote device during data transmission.
subsystem A hardware functional area of the system, such as incubation
ring, luminometer, and reagent probes. Machine controller
boards drive subsystems.
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H-14 ADVIA Centaur XP Operator’s Guide: Glossary
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Index
Numerics ancillary reagent pack
4676_1511_CPrmRgt 41 removing 24
4677_1512_CRgtShr 38 ancillary reagent queue 4, 9, 12
4678_1513_CCovers 43 anonymous results
4679_1514_CSplRck 56 defining 15
4688_1507_CAirFil 23 antibody 34
4693_1527_EmtyWaTrap 15 AElabeled 36
4694_1528_CWPrbCEjR 53 antibodycapture assay 35, 44
4695_1529_CExReagPrb 17 antigen 34, 36
4696_1530_CExAncPrb 20 aspirate probe exterior
A cleaning 22
accepting an invalid calibration 25 aspirate probes 4, 13
acid probe 4, 14 cleaning 22
Acid Reagent illustration 14
composition 13 replacing 26
acid reagent troubleshooting 58
dispensing 14 aspirated message 13
replacing 37 assay
acridinium ester 33, 35 antibodycapture 35
adding competitive 35, 38
control requests 5 reaction time 35
ADVIA Centaur system sandwich 35
topview figure 4 technology 35
AE assay problem 103, 104
antibody binding 34 assay specific factors 106
air filter assigned value card
cleaning 23 calibrator values 14
alarms assigning calibrations 25, 17
defining 38 authorized representative 1, 6, 7
ancillary barcode scanner 83 autoexclude
ancillary entry calibrator replicates 10
cleaning 43 automatic dilutions
ancillary pack 12, 20 defining 30
barcode 9 automatic runtime results report
loading 9 resetting page numbers 75
removing 9 specifying 41
storing 9 automatic sample repeats
ancillary probe 4, 12 check range 25
cleaning 20 concentration calculation range 26
ancillary queue defining 24
troubleshooting 67 defining number of replicates 28
ancillary reagent 12 holding options 29
status 18 index calculation range 26
ancillary reagent entry 9 linearity 25
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2 ADVIA Centaur XP Operator’s Guide: Index
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ADVIA Centaur XP Operator’s Guide: Index 3
defining 16 immunoassay 34
calibration status 18, 20 chemiluminescent reaction 14
cal required 16 Cleaning
current 16 aspirate probe exterior 22
due 16 cuvette waste area 43
expired 17, 20 sample time waste area 49
expired, retained 20 water bottles and reservoirs 8
historical 17, 18 cleaning
invalid 17 air filter 23
invalid, exceeded defined ranges 20 ancillary entry 43
invalid, exceeded observed ranges 20 ancillary probe 20
no master curve 17 aspirate probes 22
operator accepted 17 covers 43
valid 20 cuvette waste chute 43
valid, met retained cal limits 20 cuvette waste reservoir 43
valid, operator accepted 20 drip trays 43
valid, operator accepted, retained 21 pointing device 43
valid, retained 21 primary reagent compartment 41
waived 17, 21 probe rinse stations 34
calibrator reagent pack holders 41
autoexcluding a replicate 17 reagent probe shutter 38
display at Worklist – Summary window 42, reagent probes 17
25 rinse stations 34
excluding RLU 23 sample entry queue 43
scheduling 56, 18 sample exit queue 43
calibrator values sample racks 56
defining 14 sample tip remover 49
capacity sample tip waste chute 49
cuvette 25 sample tip waste reservoir 49
cuvette waste 25 sample tip waste tube 49
fluid waste 25 Stat entry 43
inprocess queue 6 system fluids tray 43
reagent water 25 waste probe and cuvette ejector rod 53
sample exit queue 7 water bottle and reservoir 8
sample rack 6 cleaning solution
sample tip tray waste 25 for soaking and wiping system components
sample tip trays 25 17
system fluid reservoir 37 preparing 32
tip waste 25 replacing 31
central processing unit 14 clearing cuvette jams
check digit 88 vertical cuvette chute 9
check range clearing jams
defining 25 elevator 17
chemiluminescent orientation chute 9
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8 ADVIA Centaur XP Operator’s Guide: Index
luminometer 4, 14 message
cleaning 53 Aspirated 13
troubleshooting 53, 57 Clot Detected 13
M Incomplete Cal Set 13
magnetic particles 13 Incomplete Set 13
magnets 4, 11, 13 Insufficient 13
maintaining Integrity Error 13
acid and base reagents 37 LIS Query Pending 14
cuvette loading bin 32, 33 Low Reagent 39
data files 1 No Barcode 14
sample tip waste bin 35 No Bracket 14
waste bottle 27 No Request 14
water bottle 25 Removal Pending 14
maintenance SID Mismatch 14
status 18 Stat 2
maintenance log Messages
backing up 3 Incomplete Set 13
restoring 4 messages
maintenance procedures 23 reagent status 18
Maintenance Status button 18, 19 sample status 13
managing minimizing unusable volume 2
the worklist 39 Mismatch
managing calibrations SID 52
examples 25 modem
manifold accessing files 42
cleaning 14 modifying
manually adding control reminder 19
test requests 47 reagent stability option 13
master curve sample replicates 14
calibration 1 sample replicates for automatic repeats 24
competitive assay 1 system options 37
concentrations 10 test options 9
defining 10 Modifying control reminder 19
qualitative assays 3 monitor 17
quantitative assays 1, 2 monitoring
relative light units 10 status 9
sandwich assay 2 multiple flags
specific lot 1 symbol 41
standard concentrations 1 N
standard curve 1 No Barcode message 14, 16
master curve card No Bracket message 14
barcodes 1 no calibration
master curve definition 10 calibration status 16
maximizing number of tests 2 no master curve
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14 ADVIA Centaur XP Operator’s Guide: Index
Status stat 41
system 18 time due for test completion 41
status waiting for controls 41
ancillary reagent 18 symbols 1
event log 19 event log 1
maintenance 18, 19, 18 system
monitoring 9 backing up 3
primary pack 21 bottles, specifying 44
primary reagent 8 clock, changing 40
reagents 18, 15 name, defining 38
samples 18, 13 options, modifying 37
messages 13 restoring 4
software map 25 signing in 1
supplies 18, 10 signing out 1
status button color 9 software map 24
status buttons system fluid reservoir
blinking 18 capacity 37
status message system fluids tray
calibration 18, 20 cleaning 43
stop button 5 system state
storing description 9
ancillary pack 9 System Status button 18
summary window T
type 9 T Uptake
view 9 calculating result 4
supplies defining result options 34
ordering 1 master curve card 4
status 10 ratio test 4
suspend update reference serum calibration 4
worklist 39, 43 task buttons 19
symbol technical assistance
at Status – Exit Queue window 41 printing information 5
comment 41 temperature
dilution 41 ancillary queue 9
ejected from inprocess queue 41 primary reagent compartment 8
excluded 41 troubleshooting 70
inprocess queue 41 test definition
multiple flags 41 backing up 3
repeat 41 test display order
replicate 41 modifying 36
reschedule 41 test name
result on hold 41 modifying 13
results discarded 42 test options
specific reagent 41 modifying 9
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