Saint Mary’s University

School of Health and Natural Sciences

BAYOMBONG, NUEVA VIZCAYA 3700
Nursing Department

Laboratory Activity No. 13: Analysis of Saliva

CARBONEL, Kyla Mae M.

BSN 1A

ABSTRACT

Saliva is fit for diminishing grating power by at any rate 2 requests of greatness when in the
middle of hydrophobic surfaces. This capacity to grease up is vital to oral wellbeing, sustenance
handling and taste observation. In this paper various systems of salivation grease are evaluated,
and their interconnection is shown utilizing a straightforward physical structure. The present
comprehension of the jobs of the atomic structure and physicochemical properties of major
salivary proteins and protein edifices on oil is abridged and fundamentally assessed.

Keywords: Atomic sturcture Grease, Hydrophobic, Interconnection, Physiochemical

I. INTRODUCTION

Saliva is the watery and usually somewhat frothy substance produced in the mouths of
some animals, including humans. Produced in salivary glands, saliva is 98% water, but it
contains many important substances, including electrolytes, mucus, antibacterial compounds and
various enzymes. The digestive functions of saliva include moistening food, and helping to
create a food bolus, so it can be swallowed easily. Saliva contains the enzyme amylase that
breaks some starches down into maltose and dextrin.Thus, digestion of food occurs within the
mouth, even before food reaches the stomach.( sciencedaily.com)

II. OBJECTIVES

The students will able to determine the saliva components that reacts to substances.

III. MATERIALS
 Saliva Sample
 3M HCl
 3M NaOH

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  1N HAc
 0.5 N HCl
 1% KI-starch Solution
 0.1M FeCl2
 NH4MoO4
 5% AgNO3
 Acetone
 Benedict’s Solution
 2.5 M NaOH
 0.05% CuSO4 Solution
 Filter Paper
 Litmus Paper
 Flask
 Beaker
 Funnel
 Evaporating Dish
 Test Tube
IV. PROCEDURES

1. Isolation of mucin from Saliva

Rinse the mouth with water and collect 15mL of saliva. Add 3 drops of 1N HAc and
mix. To 30 mL of acetone, in an Erlenmeyer flask, add the saliva slowly shaking the
flask after each addition. Stopper the flask and set aside for 30 minutes. Filter and
wash the precipitate formed, using 3mL portions of acetone. Allow the precipitate to
drain completely. Remove the filter paper from the funnel, spread it out in a watch
glass and allow the precipitate to dry. Use the precipitated mucin to test for the
possible components of saliva.

2. Qualitative tests for the components of saliva

a) Carbohydrates

Place about ½ of the isolated mucin in a test tube. Add 1 mL of 3mL HCl AND
IMMERSE IN A BOILING WATER BATH FOR 20-30 MINUTES. Cool and make
faintly basic with 3M NaOH, then add 3mL of Benedict’s solution. Heat again in a
boiling water bath until a visible change occurs.

b) Proteins

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 Dissolve the remaining portion of mucin in 1 mL of 2.5M NaOH, then add 3 drops of
0.05% CuSO4 solution. Mix.

c) Nitrates

Mix 1 mL of 0.05 N H2SO4. 2 drops of 1% KI and 2 drops of starch solution. Add 2

drops mL of saliva. Mix.

d) Thiocyanate

To 2 drops of saliva, add 3 drops of freshly prepared 0.1 M FeCl3 solution and a few
drops of 0.1 N HCl.

e) Glucose

Using saliva sample sample collected 15 minutes after eating, add 1mL of benedict’s
solutions to 2mL of saliva, then heat in a boiling water bath.

f) Inorganic Phosphate

Mix 1 mL of saliva and 1 mL of ammonium molybdate solution. Heat and observe.

g) Chloride

Mix 1mL of saliva with 3-5 drops of nitric acid followed by 5 drops of AgNO3
solution.

V. QUESTIONS

1. What ion is present in saliva by the results in chloride?

VI. RESULTS AND DISCUSSIONS

Table 1: Contains all the results of the saliva from different substances

Substance Tested for Test Reagents Results Conclusions

Carbohydrates NaOH, Bendicts
solution, Heat
Proteins NaOH, CuSO4
Nitrate H2SO4 KI, Starch
CNS FeCl3, HCl

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 Glucose Benedicts solution,
Heat
Inorganic Phosphate Ammonium
Molybdate
Chloride HNO3, AgNO3

Cystic fibrosis diagnosis is dependent on the chloride ion concentration in the sweat test
(≥ 60 mEq/mL – recognized as the gold standard indicator for cystic fibrosis diagnosis).
Moreover, the salivary glands express the CFTR protein in the same manner as sweat glands.
Given this context, the objective was to verify the correlation of saliva chloride concentration
and sweat chloride concentration, and between saliva sodium concentration and sweat sodium
concentration, in patients with cystic fibrosis and healthy control subjects, as a tool for cystic
fibrosis diagnosis.( sciencedirect.com)

VII. GENERALIZATION
VIII. REFERENCE

Jornal De Petria, (2018), Retrieved from

https://www.sciencedirect.com/science/article/pii/S0021755717310537

Saliva (n.d) Retrieved from https://www.sciencedaily.com/terms/saliva.htm

IX. DOCUMENTATION

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 Mucin that has been air dried

Formation of
mucin

Mucin being dissolved in the CuSo4

and NaOH

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