Вы находитесь на странице: 1из 1

Bold´s Basal Medium (BBM)

(Bold 1949, Bischoft & Bold 1963)

   

Final

 

Addition per 1 Litre culture medium

#

Component

concentration

Stock solution (g . L -1 dH 2 O)

1

NaNO 3

 

2.94

mM

25.00

10

ml

2

CaCl 2 · 2H 2 O

0.17

mM

 

2.50

10

ml

3

MgSO 4 · 7H 2 O

0.3 mM

 

7.50

10

ml

4

K

2 HPO 4

0.43

mM

 

7.50

10

ml

5

KH 2 PO 4

1.29

mM

17.50

10

ml

6

NaCl

 

0.43

mM

 

2.50

10

ml

7

Alkaline EDTA

     

solution

 

1 ml

EDTA (Titriplex III)

17.10

mM

 

50

g

 

KOH

 

55.30

mM

31

g

8

Acidified Iron

     

solution

 

1ml

FeSO 4 x 7 H 2 O

0.179

mM

 

4.98

 

H

2 SO

4

   

9

Boron solution

     

1ml

H

3 BO 3

18.50

mM

11.42

 

10

Trace Metals

     

solution

ZnSO 4 7H 2 O MnCl 2 4H 2 O MoO 3 CuSO 4 5H 2 O Co(NO 3 ) 2 6H 2 O

 

8.82

0.307 µ M

 

1ml

1.44

7.28

µ M

 

0.71

4.93

µ M

1.57

6.29

µ M

0.49

1.68

µ M

Preparation of Culture Solution

Add chemicals and stock solutions as indicated above to 1000 ml of bidistilled water. The final pH should be 6.6.