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An Investigatory Project
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Research I-B
By
March 2019
APPROVAL SHEET
Approved by the panel examiners on the oral examination with a grade of ______%
_______________________ _______________________
____________________________
i
ACKNOWLEDGEMENT
The researchers would like to extend their sincere gratitude to all who had
facilitated and supported them during the conduct of this study. Without these people,
Mrs. Jenny Rose J. Gengos, who facilitated the coming up of this successful
To the critics, Mr. Michael Jordan Estrada, Ms. Porssia Ocampo, Dra. Czarina
Aucena and Ms. Cassandra Muñoz, for taking time and effort to review and improve the
manuscript;
To the researchers’ friends, who encouraged them to strive for this requirement;
To the researchers’ parents, for their moral support, all possible assistance and
patience; and
Finally, to our Almighty God, for His inspiration, providence and provisions in
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ABSTRACT
De Sesto, E., Capua, E., Manaois, R., Comparative Study On The Bleaching Effect
Of White Vinegar And Kamias Extract. Francisco Q. Duque Medical Foundation
Special Science High School, Dagupan City, Philippines.
Bilimbi (Averrhoa bilimbi) or kamias is a sour tropical fruit abundant in vitamins B and C,
iron, phosphorus, antioxidants and oxalic acid. The latter component explains the kamias
extract’s capability to be utilized as a stain remover. Vinegar is an acidic liquid produced
through the process of fermenting ethanol alcohol into acetic acid. Its high concentration
of acetic acid makes it a great alternative in stain removal. This study is relevant to those
who are searching for a more natural, simpler and less expensive alternative to commercial
bleaches which embody chemicals that cause alarm to the safety of humans and also the
environment. The process of making this research is by way of conducting a survey to ten
respondents and taking down their evaluation in terms of effectiveness, as to which of the
alternatives is more effective. The researchers therefore conclude that kamias is indeed an
effective alternative stain remover and bleaching agent for clothing compared to other
commercial bleaches and organic stain removers.
TABLE OF CONTENTS
Abstract
Approval Sheet……..……..……………………………………………………………….i
Acknowledgement...………………………………………………………………………ii
Table of Contents................................................................................................................iii
List of Figures.....................................................................................................................v
Chapter I. INTRODUCTION
Hypotheses………………………………………………………………………...4
Conceptual Framework………….……………….………………………………..6
Research Paradigm……………………………..……………………………...…..7
Definition of Terms…………………………………..……………………………8
iii
Chapter II. REVIEW OF RELATED LITERATURE
Related Literature…………………………………………………………….…..9
Research Design………………………………………………………………....13
Materials ………………………………………………………………………...13
Procedure ……………………………………………………………...………...14
Bibliography……………………………………………………………………….…….22
Appendices ……………………………………………………………..…….………….23
iv
LIST OF FIGURES
Table 1 …………………………………………………………………………………..17
Table 2 …………………………………………………………………………………..18
Table 3 …………………………………………………………………………………..18
Table 4 …………………………………………………………………………………..19
v
CHAPTER I
INTRODUCTION
fruit-bearing tree of the genus Averrhoa, family Oxalidaceaeis, cultivated and found
throughout Indonesia, the Philippines, Sri Lanka, Malaysia and other Southeast Asian
countries. To Asian countries and islands, especially in the Philippines, its nutritional and
Many chefs find its sour flesh versatile and use it as a base or ingredient for many dishes.
Aside from its versatility, kamias is a reservoir of nutrients wherein it has significant traces
of vitamins B and C, calcium, phosphorus, iron and fiber. Also recognized for its acidity,
its extract is utilized as an alternative stain remover by locals in rural areas of the
Philippines.
White vinegar, also known as distilled or spirit vinegar, is a sour aqueous solution
of trace chemicals and acetic acid usually produced via the fermentation of foods such as
sugar beets, potatoes, molasses, milk whey or ethanol and sugars by acetic acid bacteria.
Mainly utilized in many cultures as a condiment and preservative, it also has a vast
array of industrial, medicinal, and household uses. The researchers chose to compare
vinegar with kamias due to its acidity, similar uses in cooking and capability to eliminate
stains; as well as compare the bleaching effect of white vinegar and kamias, as both are
1
readily available substances and can substitute chemical bleaches.
This study will compare the bleaching effect of white vinegar and kamias extract
on cloth stains.
removing stains on clothes in terms of the opacity of the stain after application?
stain remover?
2
Significance of the Study
This study aims to discover the stain removing potential of kamias and vinegar,
The outcome of this study shall be advantageous for those who are in search of a
more natural, simpler and less expensive alternative to commercial bleaches which contain
various chemicals that threaten the safety of humans and also the environment.
With all these bleaches, people are oblivious to what is best and less hazardous.
Thus, the researchers chose to compare these two organic stain removers.
3
Hypotheses
kamias.
kamias.
4
Scope and Delimitation
This study was conducted at the Francisco Q. Duque Medical Foundation Special
Science High School Biology Laboratory. The study concentrated in the comparison of the
bleaching capabilities of white vinegar and kamias extract as a bleaching agent on fabric
specifically on white polyester. Likewise, the effectivity shall be measured on actual utility
results.
5
Conceptual Framework
In this study, the researchers would want to discover the capacity of both vinegar
and kamias extract as a stain remover, revealing their bleaching capabilities and ultimately,
6
Research Paradigm
7
Definition of Terms
Averrhoa - a genus of trees in the Oxalidaceae family, of the Oxalidales order, named
Bleach - the generic name for any chemical product which is used industrially and
Extract - a substance made by extracting a part of a raw material, often by using a solvent
Acetic acid - an organic chemical compound best recognized for giving vinegar its sour
Stain removal - the process of removing a mark or spot left by one substance on a
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CHAPTER II
RELATED LITERATURE
VINEGAR
Vinegar can be defined as "a sour liquid obtained by result through acetic
from being utilized widely for said purpose, vinegar also has versatility as it may be used
for other purposes, such as to enhance food taste. Vinegar is made through two stages of
biological processes. These distinct processes resulted from the action of innocuous
(carbohydrates) into acetic acid. The first stage of process is called alcoholic fermentation.
It occurs when yeasts convert natural sugars to alcohol under controlled conditions. In the
second stage , "Acetobacter" which is a group of bacteria, changes the alcohol resulting
from alcoholic fermentation to acid. This is the acid fermentation that forms vinegar. Since
vinegar can be made from anything with sugar, there are probably too many variants to
count and made in countries throughout the world.[1](Kellen, 2005, Vinegar Institute Site)
White Vinegar helps remove limescale and can be used in place of fabric
conditioner; ideal for people with skin sensitivities. It will stop fluffy towels from going
stiff and helps keep your washing machine healthy by fighting limescale
and mould/mildew. Like conditioner, it will of course help prevent static. Simply use as
9
much as you would of fabric conditioner and pour it in the fabcon slot. It's also ideal for
AVERRHOA BILIMBI/KAMIAS
The bilimbi, Averrhoa bilimbi, L., (Oxalidaceae), is closely allied to the carambola
but quite different in appearance, manner of fruiting, flavor and uses. The only strictly
English names are "cucumber tree" and "tree sorrel", bestowed by the British in colonial
times. "Bilimbi" is the common name in India and has become widely used. In Malaya, it
Indias. To the French it is carambolier bilimbi, or cornichon des Indes. Filipinos generally
call it kamias but there are about a dozen other native names.
The bilimbi is generally regarded as too acidic to eat raw, but in Costa Rica, the
green, uncooked fruits are prepared as a relish which is served with rice and beans.
Sometimes it is an accompaniment for fish and meat. Ripe fruits are frequently added to
curries in the Far East. They yield 44.2% juice having a pH of 4.47, and the juice is popular
10
for making cooling beverages on the order of lemonade.
Mainly, the bilimbi is used in place of mango to make chutney, and it is much
preserved. To reduce acidity, it may be first pricked and soaked in water overnight, or
soaked in salted water for a shorter time; then it is boiled with much sugar to make a jam
or an acid jelly. The latter, in Malaya, is added to stewed fruits that are oversweet. Half-
ripe fruits are salted, set out in the sun, and pickled in brine and can be thus kept for 3
months. A quicker pickle is made by putting the fruits and salt into boiling water. This
Very acidic bilimbis are employed to clean the blade of a kris (dagger), and they
serve as mordants in the preparation of an orange dye for silk fabrics. Bilimbi juice, because
of its oxalic acid content, is useful for bleaching stains from the hands and rust from white
11
RELATED STUDIES
According to the research, there was need to find an alternative way to remove
rusting from any other metal. Despite the vast research regarding rusting, many people
still do not recognize its harmful effects to the environment and thus, do not to take
precautionary measures to avoid or prevent rusting. Results of study show that the U.S.
had to spend total $276 billion, approximately 3.1% of the nation's Gross Domestic Product
Also according to the same, vinegar is a substance that has the potential to remove
the rust stain from metal due to presence of acid in its content.
According to the research, the discovery of the potential of kamias extract as stain
remover will be a breakthrough and will contribute additional knowledge in the field of
alternative stain remover, though it still needs a little scrubbing before some stains
disappear. The study found out that kamias has high oxalic acid content which removed
12
CHAPTER III
RESEARCH METHODOLOGY
RESEARCH DESIGN
Experimental Research was used in this study wherein ten (10) respondents were
MATERIALS
cups, white fabric, filter paper, white vinegar, kamias, chocolate, ink and ketchup
13
PROCEDURE
2. Using the mortar and pestle to pound/squeeze the kamias to extract its juice
3. Using the filter paper to completely get the extract for it to be measured and stored.
1. Before using the solution the researchers will generously apply the staining materials
2. After the stain has been applied, the vinegar will first be tested by soaking the fabric
3. The researchers will also be test the kamias by soaking the fabric within an hour in 25
ml of kamias extract.
4. The researchers will observe the effects before soaking it in and will then evaluate it
5. The researchers will then scrub the fabrics in thirty (30) seconds.
6. After the two stained fabrics are scrubbed, the fabrics will then be scrutinized and
observations be recorded.
14
TESTING AND EVALUATION
The products were evaluated by ten (10) respondents who have examined the
fabric before and after application. After examination of the fabrics soaked in vinegar and
kamias extract, the respondents were asked to evaluate the effectiveness of its bleaching
effect.
effectiveness in removing the stain and the length of time for it to take effect.
5 Excellent
4 Very satisfactory
3 Satisfactory
2 Fair
1 Poor
15
TREATMENT OF DATA
The chosen respondents will evaluate the effectiveness of the bleaching agents
using the evaluation card y - the effectiveness in terms of removing stains and the average
The data gathered by the researchers was analyzed using frequency counts,
Where in:
F= frequency
X= arbitrary weights
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CHAPTER IV
The polyester was soaked, scrubbed and air-dried were air-dried before
evaluation. It was evaluated based on the effects of bleaching among 4 different stains. It
1 = poor. The weighted mean was computed and the rate of acceptability was concluded.
(2) y (4)
17
Table 1 shows that both have provided the bleaching effect desired but vinegar is
considered to be better as it was rated highly acceptable, while only acceptable for the
(2) y (4)
Table 2 shows that both solutions have not achieved the desired bleaching effect
as the 2 were rated as not acceptable on the removal of ink stain on polyester.
(2) y (4)
Table 3 shows that both solutions provided the bleaching effect desired but
kamias has a higher score than the vinegar in the removal of chocolate stain in polyester.
18
Table 4 . Rust stained polyester
(2) y (4)
Table 4 shows that both have provided the bleaching effect desired but kamias
extract is considered to be better as it was rated highly acceptable, while only acceptable
19
CHAPTER V
Summary
Science High school and was focused on the significant difference on bleaching effects of
The researchers evaluated the bleaching effects of the kamias and vinegar to four
different stains which is ketchup, ink, chocolate and rust stains. The fabric used was
polyester. The experiment had a total of 8 experiments. We applied the chocolate and rust
by rubbing against the cloth and by using a dropper, the ink was applied. Through
Conclusion
After testing the kamias and vinegar on their bleaching effects on different stains
on 2 types of fabrics and having respondents evaluate the results, the researchers therefore
conclude that the respective organic bleaches can be utilized in removing stains and their
20
Recommendations
The researchers would like to recommend that further studies on the comparison between
the bleaching effects of kamias and vinegar be done. Furthermore, the researchers suggest to use
other solutions to compare it to such as commercial detergents and to use it with higher amount.
21
BIBLIOGRAPHY
https://www.fondazioneslowfood.com/en/ark-of-taste-slow-food/kamias-
2/?fbclid=IwAR0jT_m_1YdukC7BKatFWXUbz2vdLuw0V-cYtb6q6rnIS4rXtt14nntMp8k.
from http://www.newworldencyclopedia.org/p/index.php?title=Vinegar&oldid=993522.
White Vinegar. (2018, January 27). Dri Pak,. Retrieved from https://www.dri-
pak.co.uk/tag/white-vinegar/.
https://www.hort.purdue.edu/newcrop/morton/bilimbi.html.
Essays, UK. (2018, January 4). Vinegar as a Rust Remover | Experiment. Retrieved from
https://www.ukessays.com/essays/biology/potential-of-vinegar-as-rust-stain-remover-biology-
essay.php?vref=1.
remover/.
And Lead As Substitute Pigments In Producing Carbon Papers. Tapuac District, Dagupan City:
Francisco Q. Duque Medical Foundation Special Science High School Lyceum Northwestern
University.
22
APPENDICES
T- test (1)
Treatment 1 Treatment 2
Vinegar(X) (X- 𝑋̅) (X- 𝑋̅)2 Kamias (X) (X- 𝑋̅) (X- 𝑋̅)2
4.5−3.3
T= 2 2
= 3.10284
3.3525 3.3525 √(0.61) +(1.06)
𝑆1 = √ 10−1 = √ = 0.613277808 10 10
9
= | 2.101 |
23
T- test (2)
Treatment 1 Treatment 2
1.8−1.6
T= 2 2
= 0.598
7.6 7.6 √(0.92) +(0.52)
𝑆1 = √10−1 = √ 9 = 0.92 10 10
= | 2.101 |
24
T- test (3)
Treatment 1 Treatment 2
3 0.5 0.25 3 0 0
2 -0.5 0.25 2 -1 1
3 0.5 0.25 4 1 1
4 1.5 2.25 3 0 0
3 0.5 0.25 4 1 1
2 -0.5 0.25 2 -1 1
2 -0.5 0.25 4 1 1
2 -0.5 0.25 2 -1 1
3 0.5 0.25 4 1 1
1 -1.5 2.25 2 -1 1
2.5−3
T= 2 2
= -1.342
4.5 4.5 √(0.71) +(0.94)
𝑆1 = √10−1 = √ 9 = 0.71 10 10
= | 2.101 |
25
T- test (4)
Treatment 1 Treatment 2
3.5−4.3
T= 2 2
= -2.142
6.5 6.5 √(0.85) +(0.82)
𝑆1 = √10−1 = √ 9 = 0.85 10 10
= | 2.101 |
26
Main Materials
Staining materials
27
Preparing the fabric
28
All stained fabrics
29
Extracting the kamias
30
Scrubbing the soaked fabrics for 30 seconds
Results of the dried polyester fabric that were treated with kamias
31
Results of the dried polyester fabric that were treated with kamias
32
Survey of respondents
33