CHEM 1004 Laboratory

Schedule and Supplement

Spring 2017

Laboratory Section

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Instructor’s Name

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Instructor’s Office Hours

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Instructor’s Office Location

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Instructor’s Email Address

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Do you have your safety glasses?

 CHEM 1004 LABORATORY SCHEDULE SPRING 2017
Date Experiment
Check-In and Discussion of Laboratory Safety
Jan 30 – Feb 3 Take Home Assignments:
EXP 600 Practicing Safety in the Chemistry Laboratory
EXP 614 Writing, Interpreting, and Balancing Chemical Equations

Feb 6 – Feb 10 EXP 492 Separating Food Dyes by Paper Chromatography

Feb 13 – Feb 17 Isomers and Polymers Worksheet

Feb 20 – Feb 24 Labs Cancelled for President’s Day

Feb 27 – Mar 3 EXP 628 Preparing Aspirin

Mar 6 – Mar 10 EXP 238 Determining Ascorbic Acid Content in Vitamin C Tablets

Mar 13 – Mar 17 Labs Cancelled for Spring Break

Mar 20 – Mar 24 EXP 239 Classifying Carbohydrates

Mar 27 – Mar 31 Nutritional Chemistry Worksheet

Apr 3 – Apr 7 EXP 241 Rennin Activity

Apr 10 – Apr 14 EXP 2232 Extraction of DNA from Wheat Germ

Apr 17 – Apr 21 EXP 470 Preparation and Identification of Esters

Apr 24 – Apr 28 Make up lab for EXCUSED ABSENCES

CHEM 1004 LABORATORY SUPPLEMENT Spring 2017

COURSE DESCRIPTION: Continuation of CHEM 1003. Contemporary topics in physical, biological,

and medical science. CHEM 1003 is not prerequisite to CHEM 1004.

REQUIRED MATERIALS:
• The laboratory manual is "CHEM 1004 George Washington University" Compilation by Cengage
Learning. ISBN: 9781337032124. GWU Bookstore listing: “CHEM 004 Lab Manual (Custom).”
• A pair of safety glasses or safety goggles.

LEARNING OUTCOMES:
• Demonstrate knowledge of materials learned in CHEM 1004 lectures.
• Demonstrate proper laboratory safety.
• Demonstrate proficiency in the use of modern instrumentation.
• Analyze data to reflect the precision and accuracy of the experimental procedure.
• Know and use the proper procedures for safe handling and use of chemicals. Follow proper
procedures for handling chemical waste.
• Summarize and communicate the results of your work to chemists and non-chemists.

DETERMINATION OF THE FINAL LABORATORY GRADE:

The laboratory grade will be determined according to the following point distribution.
EXP 600 Safety Quiz 50 points
EXP 614 (Take Home) 100 points
EXP 492 100 points
Isomer and Polymers Worksheet 50 points
EXP 628 100 points
EXP 238 100 points
EXP 239 100 points
Nutritional Chemistry Worksheet 50 points
EXP 241 100 points
EXP 2232 100 points
EXP 470 100 points
Lab Conduct Score from your Lab Instructor 200 points
Your final grade will be reported as the percentage of points that you earned out of the total of 1150
possible points.

Laboratory grades count as 20% of the overall CHEM 1004 course grade. In order to obtain a
passing grade in the CHEM 1004 course, you will need to complete the laboratory requirement and
obtain a passing grade for it. A passing grade is 60% or higher.

GRADING: If you have any questions about your graded reports or homework, contact your instructor
within ONE WEEK of having the assignment returned to you. After that time, no grading alterations will
be made.

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 COURSE POLICIES

ACADEMIC INTEGRITY CODE: Academic dishonesty is defined as cheating of any kind, including
misrepresenting one’s own work, taking credit for the work of others without crediting them and without
appropriate authorization, and the fabrication of information. For the remainder of the code,
see: http://www.gwu.edu/~ntegrity/code.html

ALLOWED LAB MATERIALS: When working in the lab, you are only allowed to have certain
materials with you: your lab manual and supplement, safety glasses or goggles, writing utensils, and
calculators. All other materials must be stowed in the cubes in the laboratory for the entire period. Cell
phones, laptops, iPods, and other electronic devices are NOT allowed to be on or at the lab bench
(even for use as a calculator). Use of such items in the laboratory will result in a reduction of lab
conduct points.

ATTENDANCE: Lab attendance is mandatory. Absences are only permitted for exigent circumstances,
and need to be approved by your lab instructor IN ADVANCE to count as excused. Students with
excused absences will be allowed to make up a maximum of 3 laboratory classes during the designated
make up period at the end of the semester. Acceptable reasons (with proper documentation) for an
absence include hospitalization, death of a family member, religious holiday, or a GWU sanctioned
athletic/academic commitment. If you fail to notify your laboratory instructor IN ADVANCE the absence
will be considered UNEXCUSED. Students with unexcused absences will not be allowed to make up
missed work. Each unexcused absence counts as a ZERO. THREE unexcused absences will result in an
F for the CHEM 1004 course.

In case of an emergency or illness when advance notice is not possible, contact your lab instructor
WITHIN 7 DAYS of the absence with appropriate documentation (doctor’s note, etc.). An absence that is
not reported within 7 days is considered UNEXCUSED. Also, an absence reported within those 7 days
that was not due to an emergency or illness will be considered UNEXCUSED as it was not reported in
advance. Your lab instructor will give those with excused absences make up lab instructions.
NOTE: If you are absent for any reason, it is your responsibility to make sure that your work is still
turned in on time. All work is still due on the date assigned.

CALCULATIONS: When filling out your lab reports, ALWAYS SHOW YOUR WORK. This
includes all calculations and the corresponding units. Failure to show your work will result in a loss of
points on the lab report. The only time you will not have to show your work is if your lab instructor
specifies that it’s not required.

DRESS CODE: It is mandatory to wear appropriate laboratory attire. This includes: full length pants
(no shorts, skirts, or capris), sleeved shirts (no tanks), and shoes that cover the ENTIRE top of the foot
(tennis shoes or sneakers; no flats). Long hair must be tied back. The wearing of ties, long jewelry,
scarves, etc. is not permitted, as they may get caught in equipment and/or be disruptive to the lab. If you
arrive to lab dressed inappropriately, you will have points deducted from your lab conduct score and will
be asked to correct the problem. You will be asked to leave the lab if the problem is not corrected.
Removal will be treated as an unexcused absence and result in a zero for the day’s experiment.

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EQUIPMENT: Each week the equipment necessary to complete the experiment will be found in drawers
on several carts located in the laboratory. Each pair of lab partners will sign out a drawer on the Cart
Sign-Out sheet. Students are responsible for the equipment while it is in their possession, and will have to
pay to replace any items that are found broken, damaged, or missing. TAs will check the contents of the
drawer at the end of each lab period. Any money owed must be paid using Colonial Cash to the
Chemistry Department Stockroom by the last day of the laboratory course.

LAB REPORTS: Accompanying each experiment is a data sheet(s). All observations and data are to be
recorded on the report sheet as the work is being completed. The finished lab report is to be turned in at
the end of the laboratory period. Graded lab reports will be returned during the following laboratory
period. All of the assignments for the laboratory should be completed INDIVIDUALLY. Data collection
may be done with a laboratory partner, but any calculations, analysis, and pre-lab questions should be
completed INDEPENDENTLY.
PLEASE NOTE THAT COPYING OR COLLABORATING ON A LABORATORY REPORT IS
CONSIDERED AN ACT OF ACADEMIC DISHONESTY AND MAY HAVE VERY SERIOUS
CONSEQUENCES. THE CHEMISTRY DEPARTMENT SUPPORTS THE UNIVERSITY CODE
OF ACADEMIC INTEGRITY.

LATE LAB REPORTS: Turning in late work is strongly discouraged. If necessary, the penalty for late
lab reports is as follows: Less than 24 hours late, 5% downgrade; 1 day late, 10% downgrade; 2-4 days
late, 25% downgrade; 5-7 days late, 50% downgrade. Laboratory reports will not be accepted after one
week. Late lab reports are to be turned in directly to your lab instructor during office hours or
during a designated meeting. Work turned in to the Chemistry Department Office will NOT be
accepted. Please keep in mind, it is YOUR responsibility to see that your lab instructor receives your
work if it is turned in late.

LATENESS: If you are late to lab, this will result in a reduction of your lab conduct score. If you are
more than 15 minutes late for the laboratory, you will not be permitted to attend. This will be viewed as
an unexcused absence and will count as a zero.

PRE-LABORATORY ASSIGNMENTS: You will be required to complete a Pre-Laboratory

Assignment for most laboratories. These assignments are designed to acquaint you with the procedures
and calculations that will be needed for you to understand the day's experiment. The Pre-lab questions are
turned in at the very beginning of the laboratory period on the day that the experiment is to be completed.
Pre-labs not turned in within the first 15 minutes of the lab period will not be accepted. This will
result in a zero for the assignment.

PRESENCE OF FOOD OR DRINK: Food and drink are NEVER permitted in the laboratory.
Eating/drinking is also strictly forbidden in SEH B1100 & B1700. If you attend the lab with food or
drink, you will be asked to leave. This will be treated as an unexcused absence that will result in a zero.

RELIGIOUS HOLIDAYS: You will be granted an excused absence for the observance of religious
holidays, which are recognized by the University. An excused absence means that you will be given the
right to make up the work that you miss. It is your responsibility to inform the instructor prior to
February 10, 2017 of holidays which you plan to observe, so that missed work may be rescheduled.

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Failure to do so will result in the loss of the “excused status”. A copy of the GWU Religious Holiday
Calendar is attached with dates for the current academic year.

SAFETY GLASSES/GOGGLES: Safety glasses or safety goggles are mandatory and must be worn at
all times in the laboratory. Safety glasses or goggles may be purchased at the GW Bookstore, online, or at
local hardware stores. Improper usage will result in a deduction of lab conduct points. Failure to comply
with this rule will result in your removal from the laboratory. Uncompleted experiments will be treated as
unexcused absences, resulting in a zero for the experiment.

SECURITY: In the case of an emergency, if at all possible, the class should shelter in place. If the
building that the class is in is affected, follow the evacuation procedures for the building. After
evacuation, seek shelter at a predetermined rendezvous location.

Support for Students Outside of the Classroom

Disability Support Services (DSS)

Any student who may need an accommodation based on the potential impact of a disability should contact
the Disability Support Services office at 202-994- 8250 in the Rome Hall, Suite 102, to establish
eligibility and to coordinate reasonable accommodations. For additional information please refer
to: https://disabilitysupport.gwu.edu/

Mental Health Services 202-994- 5300

The University’s Mental Health Services offers 24/7 assistance and referral to address students’ personal,
social, career, and study skills problems. Services for students include: crisis and emergency mental
health consultations confidential assessment, counseling services (individual and small group), and
referrals. http://counselingcenter.gwu.edu/

Lecturer Office Hours: Each CHEM 1004 lecturer holds office hours for their students to ask questions
and receive help. Check your lecture syllabus for the day/time.

TA Office Hours: Each CHEM 1004 laboratory TA holds office hours for their students to ask questions
and receive help. Check with your TA for the day/time.

Tutoring:
Alpha Chi Sigma (AXE, Chemistry Professional Fraternity):
Free chemistry tutoring Mondays, 8pm-10pm, Rome 204
One-on-one Tutors:
A list of chemistry tutors is available on the Chemistry Department website
(http://chemistry.columbian.gwu.edu/tutoring). Each tutor sets his/her own hours and
rates. Please contact individual tutors for more information.

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 CHEM 1004 ADDITIONAL INSTRUCTIONS FOR THE EXPERIMENTS
It is required that you read the additional notes prior to the experiments.

EXP 600 PRACTICING SAFETY IN THE CHEMISTRY LABORATORY

Fill out the Safety Information sheet on page 7 to keep in your laboratory manual for reference. The Safety
Agreement on page 8 does not need to be filled out and turned in. Separate Safety Contracts will be provided
in the laboratory during check-in.
The Laboratory Safety Quiz on page 9 should be completed using the information provided in the module
and turned in to your laboratory instructor. Make sure to write out all of the steps for the emergency
protocols, and to include at least 9 safety violations in Question 6.

EXP 614 WRITING, INTERPRETING, AND BALANCING CHEMICAL EQUATIONS

This take home laboratory has been included to help you with your understanding of the information provided by
balanced chemical equations. The following problem sets in EXP 614 should be completed:
Problem Set 1: Questions 1 and 2
Problem Set 2: All Questions
Problem Set 3: All Questions
Problem Set 4: Questions 1 and 3

EXP 492 SEPARATING AND IDENTIFYING FOOD DYES BY PAPER CHROMATOGRAPHY

OVERVIEW: We will learn about food dyes and paper chromatography. Then we will identify food dyes that are
in various colored commercial products. A more simplified set of instructions will be provided at your laboratory
station. We will be using 0.10% “table salt solution” as our solvent system. This laboratory requires some simple
mathematical calculations. Bring a calculator.

PROCEDURE: Please read and understand Part I. Choosing a Solvent System

Plastic wrap will be used to cover the beakers containing the chromatography solvent instead of petri dishes. A
Dye Kit will be issued to each pair of students. The Dye Kit contains B1, B2, Y5, Y6, R3, R40, and G3. This
means that Step 5 will not need to be completed. Also, the use of plastic wrap (instead of petri dishes) to cover
beakers of solvent will continue for the remainder of the experiment. You will complete the procedure according
to the instructions that will be found on your laboratory bench.
Pre laboratory Assignment All questions
To Borrow from stockroom Glassware Drawer
Disposal All solutions maybe discarded in the sink with plenty of running water
Lab Report Due Data Sheets 1 and 3

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 EXP 628 PREPARING ASPIRIN

OVERVIEW: Aspirin (acetylsalicylic acid) is a common over-the-counter analgesic. Analgesics are drugs used
to cause pain relief. In this experiment, students will synthesize aspirin by performing an acid catalyzed chemical
reaction. Students will determine the theoretical yield for their reaction and upon completion determine how
successful their reaction was by calculating the percent yield.

PROCEDURE:
EXPERIMENT WILL BE COMPLETED IN FUME HOODS DUE TO CHEMICAL HAZARDS.
Acetic anhydride will not need to be measured. A pump set up to deliver 4.0 mL will be placed on the reagent
bottle for students to add acetic anhydride directly to their reaction flask. Concentrated sulfuric acid will be in
dropper bottles in the student fume hoods. Students can add directly to their reaction flask. No need for Pasteur
pipets!

Pre laboratory Assignment All questions

To Borrow from stockroom Glassware Drawer
Disposal Filter paper and aspirin are collected in labeled waste container. Discarded
solutions will be treated following the procedure in the lab manual and
poured in the sink with running water.
Lab Report Due Data and Observations Sheet, Calculations and Conclusions

EXP 238 DETERMINING ASCORBIC ACID IN VITAMIN C

OVERVIEW: Ascorbic acid, vitamin C, is found in citrus fruits, tomatoes, and some green vegetables. Its main
function in the body is involved with the production of collagen. The consumption of vitamin C supplements is
currently being promoted because ascorbic acid is an “antioxidant”. In this experiment you will determine the
amount of ascorbic acid in a vitamin C tablet.

PROCEDURE: The procedure will be completed as written in the module. Omit part IV

Pre laboratory Assignment All questions

To Borrow from stockroom Glassware Drawer and 1 Burette
Disposal All solutions maybe discarded in the sink with plenty of running water
Lab Report Due All Data Sheets

EXP 239 CLASSIFYING CARBOHYDRATES

OVERVEIW: Carbohydrates provide energy for our bodies. In this experiment, you will learn to perform several
tests that distinguish between some common carbohydrates. Based on the results of these observations, you will
identify an unknown carbohydrate solution. A more simplified set of instructions will be provided at your
laboratory station.

PROCEDURE: Hot plates will be used for this experiment. Part V. Bial’s Test will not be completed. The
carbohydrates that will be studied are as follows: glucose, fructose, sucrose, lactose, and starch. (Ribose has been
excluded.)

Pre laboratory Assignment
To Borrow from stockroom
Disposal
Lab Report Due
Questions 1b), 2, 3a), 3b), 3c), 3d), and 4
Glassware Drawer
Completed tests should be discarded in an appropriate labeled waste
containers
Data and Observation Sheets

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 EXP 241 RENNIN ACTIVITY

OVERVEIW: Rennin is a clotting enzyme that catalyzes the formation of curds and is used in the cheese industry.
In this experiment you will learn a simple enzyme assay. This assay will then be employed to study some factors
that can affect rennin activity.

PROCEDURE: Hot plates will be used for this experiment. Follow procedure as written.

Pre laboratory Assignment All questions

To Borrow from stockroom Glassware Drawer
Disposal Completed tests should be discarded in an appropriate labeled waste
containers
Lab Report Due Data and Observation Sheets, and Assessment Worksheet

EXP 2232 Extraction of DNA from Wheat Germ

OVERVEIW: Deoxyribonucleic acid (DNA) is a type of nucleic acid, in which genetic information is stored in
most organisms. DNA is located in the nucleus of cells. In this experiment, DNA will be extracted from wheat
germ cells while using heat to denature interfering proteins. The effect of detergents on cell membranes will be
studied. Additionally DNA will be extracted from what germ cells using an enzyme to denature interfering
proteins.

PROCEDURE: Follow procedure as written.

Hot plates will be used for this experiment to heat water needed for the experiment.

Pre laboratory Assignment All questions

To Borrow from stockroom Glassware Drawer
Disposal All solutions may be poured in the sink with running water, and
toothpicks may be discarded in the trash receptacle.
Lab Report Due Data and Report Sheets, and Questions Sheets

EXP 470 PREPARATION AND IDENTIFICATION OF ESTERS

OVERVEIW: Esters are responsible for many of the familiar odors that are experienced in everyday life. The
smells associated with bananas, cherries, and pineapple will be synthesized in the laboratory. The scent that is
analogous with nail polish remover and wintergreen will also be prepared. An unknown ester will be identified by
its odor.

PROCEDURE: The procedure will be completed as written in the module.

Pre laboratory Assignment Questions 1 and 2.

To Borrow from stockroom Glassware Drawer
Disposal Completed tests should be discarded in an appropriate labeled waste
containers
Lab Report Due Data and Observation Sheets, and Assessment Worksheet

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