LH Series Host Transmission Specification

COULTER® LH Series WorkStation
Host Transmission
Specification
N E C M u ltiS y n c L C D 1 7 0 0 m +
GEN• S
PN 4277303D (September 2004)

Beckman Coulter, Inc.
Fullerton, CA 92835
WARNINGS AND PRECAUTIONS
READ ALL PRODUCT MANUALS AND CONSULT WITH BECKMAN COULTER-TRAINED PERSONNEL BEFORE ATTEMPTING
TO OPERATE INSTRUMENT. DO NOT ATTEMPT TO PERFORM ANY PROCEDURE BEFORE CAREFULLY READING ALL
INSTRUCTIONS. ALWAYS FOLLOW PRODUCT LABELING AND MANUFACTURER’S RECOMMENDATIONS. IF IN DOUBT AS
TO HOW TO PROCEED IN ANY SITUATION, CONTACT YOUR BECKMAN COULTER REPRESENTATIVE.
HAZARDS AND OPERATIONAL PRECAUTIONS AND LIMITATIONS

WARNINGS, CAUTIONS, and IMPORTANTS alert you as follows:
WARNING - Can cause injury.
CAUTION - Can cause damage to the instrument.
IMPORTANT - Can cause misleading results.
BECKMAN COULTER, INC. URGES ITS CUSTOMERS TO COMPLY WITH ALL NATIONAL HEALTH AND SAFETY
STANDARDS SUCH AS THE USE OF BARRIER PROTECTION. THIS MAY INCLUDE, BUT IT IS NOT LIMITED TO,
PROTECTIVE EYEWEAR, GLOVES, AND SUITABLE LABORATORY ATTIRE WHEN OPERATING OR MAINTAINING THIS OR
ANY OTHER AUTOMATED LABORATORY ANALYZER.
WARNING Risk of operator injury if:

r All doors, covers and panels are not closed and secured in place prior to and during instrument operation.
r The integrity of safety interlocks and sensors is compromised.
r Instrument alarms and error messages are not acknowledged and acted upon.
r You contact moving parts.
r You mishandle broken parts.
r Doors, covers and panels are not opened, closed, removed and/or replaced with care.
r Improper tools are used for troubleshooting.
To avoid injury:
r Keep doors, covers and panels closed and secured in place while the instrument is in use.
r Take full advantage of the safety features of the instrument. Do not defeat safety interlocks and sensors.
r Acknowledge and act upon instrument alarms and error messages.
r Keep away from moving parts.
r Report any broken parts to your Beckman Coulter Representative.
r Open/remove and close/replace doors, covers and panels with care.
r Use the proper tools when troubleshooting.
CAUTION System integrity might be compromised and operational failures might occur if:
r This equipment is used in a manner other than specified. Operate the instrument as instructed in the Product Manuals.
r You introduce software that is not authorized by Beckman Coulter into your computer. Only operate your system’s
computer with software authorized by Beckman Coulter.
r You install software that is not an original copyrighted version. Only use software that is an original copyrighted
version to prevent virus contamination.
IMPORTANT If you purchased this product from anyone other than Beckman Coulter or an authorized Beckman Coulter
distributor, and, if it is not presently under a Beckman Coulter service maintenance agreement, Beckman Coulter cannot
guarantee that the product is fitted with the most current mandatory engineering revisions or that you will receive the most
current information bulletins concerning the product. If you purchased this product from a third party and would like
further information concerning this topic, call your Beckman Coulter Representative.
REVISION STATUS
Initial Issue, 7/01

Software Version 1A.
Revision B, 4/02
Software Version 2A. Derived from internal Part Number 0302000G.
Updated Section 4.3, Dilution Factor field comments.
Removed all references to “+ Subset” globally.
Added WBC Threshold Full Histogram Group Fields.
Added new Section 3.18, Collate the ToDo List.
Updated General Information Group Fields from 29 groups to 24 groups.
Added notes to Tables 4-3 and 4-15, respectively.
Updated the following tables: 4-1; 4-3; 4-6; 4-8; 4-12; 4-13; 4-21; 4-30; 4-31; 4-33; 4-39;
4-41; 4-46; 4-48; 4-52; 5-1; 5-2; 5-4.
Removed NRBC note from Tables 4-24 and 4-46.
Added “Note: Parameter selection does not affect control transmissions.” to Tables 4-43;
4-44; 4-46; 4-48; and 4-50.
Updated Control Information Group 34, removed reference WBC Count Field.
Updated Section 6.2, added SOH to table.
Updated Section 8.1.
Revision C, 9/03
Software Version 2B. Derived from internal Part Number 0302000K.
Updated the following tables: 4-23; 4-24; 4-43; 4-4; 4-46; 4-48; 4-50.
Added new section 3.19.
Revision D, 9/04
LH 700 Software Version 2B and LH 500 Software Version 2A. Derived from internal Part
Number 0302000N.
Updated the following tables: 4.12; 4.20; 4.22; 4.25; 4.28; 4.30; 4.37; 5.1.
This document applies to the latest software listed and higher versions. When a subsequent software version changes the
information in this document, a new issue will be released.
PN 4277303D iii
REVISION STATUS
iv PN 4277303D
CONTENTS
REVISION STATUS, iii
1 INTRODUCTION, 1-1
1.1 PURPOSE, 1-1
2 HARDWARE INTERFACE, 2-1
2.1 HARDWARE HANDSHAKE, 2-1
3 COMMUNICATION PARAMETERS, 3-1
3.1 COMMUNICATION PARAMETERS, 3-1
3.2 COMMUNICATION PROTOCOLS, 3-1

Low Level Protocol, 3-1
High Level Protocol, 3-1
3.3 TIME-OUT, 3-2
3.4 BAUD RATE, 3-2
3.5 DATA BITS, 3-2
3.6 PARITY, 3-2
3.7 STOP BITS, 3-2
3.8 HANDSHAKE, 3-2
3.9 BLOCK SIZE, 3-2
3.10 GRAPHICS, 3-2
3.11 COMPATIBILITY, 3-3
3.12 SPOOLER, 3-3
3.13 CLEARING THE SPOOLER, 3-3
3.14 REPLACE NULL BY SP, 3-3
3.15 DISABLING HOST COMMUNICATIONS, 3-3
3.16 ENABLE VALIDATION CODES, 3-3
3.17 REPLACE DLE E WITH DLE C, 3-4
3.18 COLLATE THE TODO LIST, 3-4
3.19 REPLACE V AND V FLAGS WITH R (LH 500 ONLY), 3-4
4 LH SERIES WORKSTATION TO HOST COMMUNICATIONS, 4-1
4.1 DATA-LINK, 4-1
PN 4277303D v
CONTENTS
Full Software Handshake, 4-1

Software Handshake Action, 4-1
No Handshake, 4-2
Data Block Structure, 4-2
Group Definition Locator Table, 4-3
4.2 1G.1 + RETICS FORMAT, 4-4

Message Structure, 4-4
Preamble, 4-4
Group, 4-4
Field Count, 4-4
Fields, 4-5
Postamble, 4-5
General Information Group Fields, 4-6
CBC Parameter Group Fields, 4-9
DIFF Count Parameter Group Fields, 4-10
DIFF Percent Parameter Group Fields, 4-12
Comment Group Fields, 4-14
Flag Group Fields, 4-14
Demographics Group Fields, 4-18
DF1 DataPlot Group Fields, 4-20
DF2 DataPlot Group Fields, 4-20
DIFF (VCS) Histogram Group Fields, 4-20
RBC Histogram Group Fields, 4-21
PLT Histogram Group Fields, 4-21
RETIC Parameters Group Fields, 4-22
DF5 LS DataPlot Group Fields, 4-24
DF6 OP DataPlot Group Fields, 4-24
RETIC Histogram Group, 4-25
4.3 LH 750 WORKSTATION FORMAT, 4-25

Group Definition, 4-28
General Information Group Fields, 4-29
CBC Parameters Group Fields, 4-33
DIFF Count Parameters Group Fields, 4-35
DIFF Percent Parameters Group Fields, 4-37
Comment Group Fields, 4-38
Definitive Flags Group Fields, 4-39
Suspect Flags Group Fields, 4-40
Conditional Flags Group Fields, 4-41
Other Flags Group Fields, 4-43
Demographics, 4-43
RBC Histogram Group Fields, 4-47
PLT Histogram Group Fields, 4-47
RETIC Parameters Group Fields, 4-48
WBC Threshold Group Fields, 4-50
WBC Threshold Full Histogram Group Fields, 4-50
DIFF Latex Parameters Group Fields, 4-50
RETIC Latex Parameters Group Fields, 4-52
vi PN 4277303D
CONTENTS
Control CBC Parameters Group Fields, 4-56

Control DIFF Count Parameters Group Fields, 4-57
Control DIFF Percent Parameters Group Fields, 4-58
Control RETIC Parameters Group Fields, 4-59
DIFF Graphics, 4-61
Retic Graphics, 4-61
4.4 HOST PARSING OF TRANSMITTED DATA, 4-62
5 HOST TO LH SERIES WORKSTATION COMMUNICATIONS

(HOST ToDo LIST), 5-1
5.1 DATA-LINK, 5-1

Protocol, 5-1
Data Block Structure, 5-4
5.2 PRESENTATION, 5-4

DLE Responses From The Workstation And Effects After A ToDo List
Download, 5-5
5.3 MESSAGE DEFINITION, 5-7
5.4 DELETE MESSAGE, 5-14
6 ASCII Tables, 6-1
6.1 7-BIT ASCII CODES, 6-1
6.2 VALID HOST COMMUNICATIONS ASCII CODES, 6-1
7 CRC, 7-1
7.1 CRC ALGORITHM, 7-1
7.2 CRC EXAMPLE WRITTEN IN ASM86, 7-2
7.3 CRC EXAMPLE WRITTEN IN C, 7-3
8 GRAPHIC RECONSTRUCTION, 8-1
8.1 RBC, WBC THRESHOLD, AND PLT HISTOGRAMS, 8-1
8.2 LH 750 DATAPLOT FORMAT, 8-1

Dataplots, 8-1
Dataplot Data Header, 8-2
Dataplot Data Header Elements, 8-2
Dataplot Data Block, 8-2
Dataplot Data Block Header, 8-3
Rendering Information Block, 8-3
Rendering Information Block Header, 8-4
Color Palette, 8-4
Dithered Display Bitmap Library, 8-4
PN 4277303D vii
CONTENTS
Dataplot Header, 8-5
TRADEMARKS, 1
COULTER LH SERIES SYSTEM HARD-COPY DOCUMENTATION, 2
ILLUSTRATIONS
8.1 Plotting RBC, WBC Threshold and PLT Histograms, 8-1
8.2 Dataplot Transmission, 8-2
8.3 Dataplot Data Header, 8-2
8.4 Dataplot Data Block Format, 8-2
8.5 Dataplot Data Block Header, 8-3
8.6 Rendering Information Block, 8-4
8.7 Rendering Information Block Header Format, 8-4
8.8 Palette Table Entry, 8-4
8.9 Bitmap Format, 8-5
8.10 Dataplot Header, 8-5
8.11 Dataplot Data Format, 8-6
viii PN 4277303D
CONTENTS
TABLES
3.1 Communication Parameters Available by Format, 3-1
3.2 Graphics Options, 3-3
4.1 Group Definition Locator, 4-3
4.2 Group Definition: 1G.1 + Retics, 4-6
4.3 General Information -- Group 1, 4-6
4.4 CBC Parameters -- Group 2, 4-9
4.5 Flag Data for CBC Parameter Group Fields, 4-10
4.6 DIFF Count Parameters -- Group 3, 4-10
4.7 Flag Data for Diff Count Parameter Group Fields, 4-12
4.8 DIFF Percent Parameters -- Group 4, 4-12
4.9 Flag Data for DIFF Percent Parameter Group Fields, 4-13
4.10 Comment -- Group 5, 4-14
4.11 Flag -- Group 6, 4-14
4.12 LH 750 Workstation/STKS 1G.1 + Retics Messages, 4-15
4.13 Demographics -- Group 7, 4-18
4.14 RBC Histogram -- Group 11, 4-21
4.15 PLT Histogram -- Group 12, 4-21
4.16 RETIC Parameters -- Group 13, 4-22
4.17 Flag Data for Retic Parameter Group Fields, 4-24
4.18 Transmission Tests and Test IDs by Test Type, 4-25
4.19 Transmitted Groups, 4-28
4.20 General Information -- Group 1, 4-29
4.21 Possible Values for Sample Status, 4-33
4.22 CBC Parameters -- Group 2, 4-33
4.23 Flag Data for CBC Parameter Group Fields, 4-34
4.24 DIFF Count Parameters -- Group 3, 4-35
4.25 Flag Data for DIFF Count Parameter Group Fields, 4-36
4.26 DIFF Percent Parameters -- Group 4, 4-37
4.27 Flag Data for DIFF Percent Parameter Group Fields, 4-38
4.28 Comment -- Group 5, 4-38
4.29 Definitive Flags -- Group 6, 4-39
4.30 Suspect Flags -- Group 7, 4-40
4.31 Conditional Flags -- Group 8, 4-41
4.32 Other Flags -- Group 9, 4-43
4.33 Demographics -- Group 10, 4-43
4.34 RBC Histogram -- Group 14, 4-47
4.35 PLT Histogram -- Group 15, 4-47
4.36 RETIC Parameters -- Group 16, 4-48
4.37 Flag Data for Retic Parameter Group Fields, 4-49
4.38 WBC Threshold Monitor Group 26, 4-50
4.39 DIFF Latex Parameters -- Group 31, 4-50
4.40 Flag Data for DIFF Latex Parameters Group Fields, 4-51
4.41 RETIC Latex Parameters -- Group 32, 4-52
4.42 Flag Data for RETIC Latex Parameters Group Fields, 4-53
4.43 Control Information -- Group 34, 4-53
4.44 Control CBC Parameters -- Group 35, 4-56
4.45 Flag Data for Control CBC Parameters Group Fields, 4-57
4.46 Control DIFF Count Parameters -- Group 36, 4-57
PN 4277303D ix
CONTENTS
4.47 Flag Data for DIFF Count Parameter Group Fields, 4-58
4.48 Control DIFF Percent Parameters -- Group 37, 4-58
4.49 Flag Data for Diff Percent Parameter Group Fields, 4-59
4.50 Control RETIC Parameters -- Group 38, 4-59
4.51 Flag Data for Control RETIC Parameter Group Fields, 4-61
4.52 DIFF DataPlot -- Group 50, 4-61
4.53 Retic DataPlot -- Group 51, 4-61
5.1 Message Definition Table, 5-7
5.3 Possible Combinations of Sample Requests with Multiple Test Requests, 5-13
5.2 Possible Values and Examples of Test Requests, 5-13
5.4 Patient ID Deletions, 5-16
x PN 4277303D
1INTRODUCTION 1
1.1 PURPOSE
This document is the specification for communication between the LH Series Workstation
and a host computer. The functionality will remain backward compatible. This document is
intended for use by software engineers needing to either maintain or modify the operation of
the LH Series Workstation Host Communications.
This document describes the transfer of data from the LH Series Workstation to an LIS/HIS
computer in the following formats:
r STKS 1G.1 + Retics

r LH 750 Workstation: host communication protocol which supports transmission of
CBC, DIFF and Reticulocytes parameters for samples and control runs as well as
collated sample runs. This format is an extension of the STKS 2A and 3A formats.
The LH 750 “STKS 1G.1 + Retics” format is provided for use with existing STKS™ and
MAXM™ interfaces. Due to the LH Series Workstation’s new features and enhancements,
STKS and MAXM instrument formats are not completely supported (see details in following
sections).
PN 4277303D 1-1
INTRODUCTION
PURPOSE
1-2 PN 4277303D
2HARDWARE INTERFACE 2
The system is equipped with a Host connector on the rear of the LH SeriesWorkstation that
lets the system interface with a host computer via a Standard EIA-type 25-pin connector and
uses EIA Standard RS-232-C signals. The LH 500 is equipped with a 9 pin to 25 pin adapter
allowing the the system to interface with a host computer. This is a Data Terminal Equipment
(DTE) configuration.
The LH SeriesWorkstation/Host communications uses only the following signals:
P3 Pin No. Signal Name Flow Direction

2 Transmit Data From LH Series Workstation
3 Received Data To LH Series Workstation
4 Ready To Send (RTS) From LH Series Workstation
5 Clear To Send (CTS) To LH Series Workstation
6 Data Set Ready (DSR) To LH Series Workstation
7 Signal Ground Reference
8 Carrier Detect Reference
20 Data Terminal Ready (DTR) From LH Series Workstation
22 Ring Indicator Reference
The LH SeriesWorkstation is the DTE, by RS-232 standards, which explains the RTS/DTR
pin-outs. Whether the HOST is a DTE or DCE will determine its use of CTS/DSR or RTS/DTR.
A DTE HOST requires the use of a NULL MODEM Cable.
Note: Pin number 5 CTS must be active for LH Series Workstation to send any transmissions.
If the host computer does not support the above hardware handshake lines, it will be
necessary to connect pin 4 to pin 5 and to connect pin 20 to pin 6.
2.1 HARDWARE HANDSHAKE

During a transmission, the host logically raises CTS to allow the LH Series Workstation to
send data and logically lowers CTS to prevent the LH Series Workstation from sending data.
Note: If CTS is lowered to hold off the LH Series Workstation host transmission, it must be
raised again within the Workstation time-out period. If CTS is not raised within the time-out
period, the LH Series Workstation transmission to the host will time-out and reset, ready for
the next transmission.
PN 4277303D 2-1
HARDWARE INTERFACE
HARDWARE HANDSHAKE
Diagram of Hardware Handshake Mode
LH Series Workstation (SENDER) Host (RECEIVER)

<-- CTS on (send me data)
send data ----->
send data ----->
send data ----->
<-- CTS off (stop sending data)
wait
• process received data
• •
• •
• •
•
<-- CTS on (send more data)
send data ----->
send data ----->
- etc.-
2-2 PN 4277303D
3COMMUNICATION PARAMETERS 3
3.1 COMMUNICATION PARAMETERS
The LH SeriesWorkstation allows the user to configure the following communication
parameters on the Host Computer Definition screen. These parameters include
communication modes, as well as parameters enabling the transmission of graphic data.
3.2 COMMUNICATION PROTOCOLS

The transmission specification consists of data-link (low level) and presentation (high level)
protocols. Data-link protocol provides the means of transmitting data without any regard to
actual information. Presentation protocol describes the actual information and its format.
Low Level Protocol

The Host Low Level Communication Protocol will be supported by all formats.
High Level Protocol

The High Level Communication Protocols currently supported are the
“STKS 1G.1 + Retics” and “LH 750 Workstation”. The High Level Communication Protocol
is selected using the compatibility field.
Note: To satisfy the host communication requirements, the receiver MUST parse for all of the
data.
DO NOT ASSUME FIXED OFFSETS
Table 3.1 lists the communication parameters available by format.

Table 3.1 Communication Parameters Available by Format
Communication Parameter 1G.1 + Retics LH 750 Workstation

Time-out X X
Baud Rate X X
Data Bits X X
Parity X X
Stop Bits X X
Handshake X X
Block Size X X
Graphics X X
Compatibility X X
X in 1G.1 + Retics column = Supported by the LH 750 “STKS 1G.1 + Retics ” format.
X in LH 750 Workstation column = Supported by the LH 750 Workstation format.
PN 4277303D 3-1
COMMUNICATION PARAMETERS
TIME-OUT
3.3 TIME-OUT
The time-out value determines the amount of time the LH SeriesWorkstation waits for a
response from the host before retrying to send data to the host. The LH SeriesWorkstation
continues to transmit at the time-out intervals until the host successfully receives the data.
The time-out values are 30, 60, and 90 seconds. The default value is 90 seconds.
3.4 BAUD RATE

The following baud rates are supported: 2400, 4800, 9600, 19200 and 38400.
Note: The minimum recommended baud rate is 2400 for non-graphic transmissions and
9600 Baud for graphic transmissions.
3.5 DATA BITS

Host Communication can be configured for 7 or 8 bit mode.
3.6 PARITY
Odd, Even, and Off (None) parity modes are supported. Odd parity is the default value.
3.7 STOP BITS

None, 1, or 2 Stop Bit modes are supported. The default value is 2.
3.8 HANDSHAKE
This option determines whether or not the LH SeriesWorkstation looks for a handshake from
the host computer. See Heading 4.1, Data-Link, for additional information.
3.9 BLOCK SIZE

The LH SeriesWorkstation/Host Communications support block sizes of 128 and 256 bytes.
The default value is 256 bytes.
3.10 GRAPHICS
The LH SeriesWorkstation allows DIFF and Retic graphic transmission if the compatibility
format selected is the LH SeriesWorkstation format.
Table 3.2 lists the graphic items that can be enabled or disabled for the corresponding test
based on the current compatibility format and report profile selected.
3-2 PN 4277303D
COMPATIBILITY 3
Table 3.2 Graphics Options
Test Graphics 1G.1 + Retics LH 750 Workstation

CBC RBC Histogram X X
PLT Histogram X X
WBC Threshold N/A X
DIFF DataPlot N/A X
RETICS DataPlot N/A X
Subset DataPlot N/A X
X in 1G.1 + Retics column = Supported by the LH 750 STKS 1G.1 + Retics ” format.
X in LH 750 Workstation column = Supported by the LH 750 Workstation format.
3.11 COMPATIBILITY
This option determines which transmission format is used by the LH SeriesWorkstation to
send results to an LIS/HIS computer. The Host High Level Communication Protocols
supported are: “STKS 1G.1 + Retics” and “LH 750 Workstation”.
3.12 SPOOLER
Transmissions to the Host are spooled before sending. When a transmission is sent to the
Host, it will be kept on the Spooler and resent until the Host acknowledges that the
transmission was successfully received. If the Host is offline when Transmit To Host is
enabled, all transmissions intended for the Host will be kept on the Spooler until the Host
comes online or the Host Spooler is cleared.
3.13 CLEARING THE SPOOLER

Select the Clear button in the LIS folder on the System Run Configuration screen to clear all
spooled transmissions intended for the Host. It should be remembered that new
transmissions to the Host will still be subsequently spooled. See Section 3.15 to disable host
communications and hence the host spooler.
3.14 REPLACE NULL BY SP

This is no longer an option because NULL characters are not included in the transmission
buffer.
3.15 DISABLING HOST COMMUNICATIONS

Select the Disable button in the LIS folder on the System Run Configuration screen to disable
all auto transmissions from the LH SeriesWorkstation to the Host. This will not affect manual
transmissions or transmissions from the Host to the LH SeriesWorkstation.
3.16 ENABLE VALIDATION CODES

This checkbox enables the codes used to identify the validation status of individual test
requests on a sample as detailed in Conditional Flags Group Fields under AutoValidation in
Chapter 4. If “Enable Validation Codes” in System Setup - [Communications] is checked
(default), the individual test group validation status will be transmitted. Applied only to
LH 750 format.
PN 4277303D 3-3
REPLACE DLE E WITH DLE C
3.17 REPLACE DLE E WITH DLE C

Select this checkbox to replace DLE E with DLE C in the standard data set transmitted to your
information system.
3.18 COLLATE THE TODO LIST

The ToDo List generation is derived from task pre-assignment, by determining what sets of
data are to be collated into a final report, test panels are pre-assigned either manually or by
LIS download.
3.19 REPLACE V AND V FLAGS WITH R (LH 500 ONLY)

If transmitting in the protocol “STKS 1G.1 + Retics” all transmissions of V and v flags will be
sent as R. If transmitting in the “LH 750 Workstation” protocol, transmissions of V and v flags
will be sent as R only if the “Replace V and v flags with an R” option is enabled, otherwise V
and v flags will be sent as V and v.
3-4 PN 4277303D
4LH SERIES WORKSTATION TO HOST COMMUNICATIONS 4
4.1 DATA-LINK
A sample transmission to the Host consists of one or more framed data blocks. See Data Block
Structure below. All transmitted data bytes are ASCII characters. All numeric values are ASCII
representations of hexadecimal numbers. Hence, for example, the number FF (hex) is
represented by the two ASCII bytes "FF" (46H, 46H). The first two bytes of the transmission
are the number of blocks to be sent, in ASCII, followed by transmission of data blocks (see
format below).
The final block will be padded with ASCII spaces (20H) to fill a whole block.
The LH Series Workstation supports “full software handshake” and “no software handshake”
protocols.
Full Software Handshake

The LH Series Workstation sends the following control characters plus data and expects the
indicated host response:
LH Series Workstation (SENDER) HOST (RECEIVER)

SYN (ready)------>
<-------SYN (go ahead)
Block Count------>
<-------ACK (ready to receive)
or
NAK (receiver abort)
send data blocks
Block ------>
for each block <-------ACK (block received ok)
• or
• NAK (retransmit this block)
• or
• SYN (retransmit all blocks)
SYN (all done)------>
<-------ACK (transmission accepted)
Up to 255 blocks of data can be sent. The actual number of blocks sent is specified by the
two-byte ASCII Block Count.
Software Handshake Action

If the host NAKs a data block, the block is retransmitted. It is up to the host to determine how
many times it retries receiving a NAKed block before aborting. Typically, the Host will NAK a
datablock if the current block number is incorrect(e.g. out of sequence), the Host’s calculated
CRC does not match the transmitted block’s CRC or block framing is not recognized as
correct.
A SYN sent by the Host after a block has been transmitted by the LH Series Workstation forces
the Workstation to retransmit all data starting with the first block. This does not include the
PN 4277303D 4-1
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
DATA-LINK
initial SYN and block count. It is up to the Host to determine under what conditions to
transmit a SYN.
The LH Series Workstation continues to send the same sample transmission until the Host
accepts it entirely (ACKs each block). To abort a transmission, the host must ACK each block
of the transmission and discard it locally.
No Handshake
If “Handshake” option is disabled, the protocol ignores all hardware and software host
hanshake responses. In addition, in the No Handshake mode, the LH SeriesWorkstation does
not send a SYN prior to transmitting the data. CTS is always used to indicate that a Host is MS
and turned on.
Data Block Structure
Byte # # of bytes
1 STX 1 byte
2 BLK NBR MS CHAR 1 byte
3 BLK NBR LS CHAR 1 byte
4 DATA BYTES
• 256 (128) C
bytes R
• C
259 (131) •
260 (132) CRC MSB MS CHAR 1 byte
261 (133) CRC MSB LS CHAR 1 byte
262 (134) CRC LSB MS CHAR 1 byte
263 (135) CRC LSB LS CHAR 1 byte
264 (136) ETX 1 byte
Each data block is framed with a Start Of Text(STX = 02H) and an End Of Text(ETX =03H)
control character.
The next two bytes after STX have the Current Block Sequence Number(BLK NBR) in ASCII
starting with the number 1(MS byte = 30H, LS byte = 31H, to give ‘01’) for the first block. The
block number of the last block must equal the Block Count sent at the beginning of the
transmission.
Every data block will have either 128 or 256 bytes (whichever is chosen). If there is
insufficient data to fill a block, it will be padded with space characters (last block
transmitted).
The CRC for the block’s data is calculated by the workstation and sent as the last four bytes of
the block before the ETX in the ASCII format shown above. The Most Significant Byte (MSB)
is generated as a Most Significant and Least Significant ASCII character (MS and LS CHAR).
The same is true for the Least Significant Byte (LSB).
4-2 PN 4277303D
DATA-LINK 4
The algorithm used to calculate the CRC for each block is a modified CCITT CRC16. CRC is
only calculated on the data bytes.
Chapter 7 details the CRC algorithm and includes application notes for implementing the
algorithm in both C and assembly language.
Group Definition Locator Table

Table 4.1 lists the group names for each format.
Table 4.1 Group Definition Locator
LH 750
1G.1 + Retics, Group Workstation,
Group Name Group Number Heading 4.2 Number Heading 4.3
General Information 1 X 1 X
CBC Parameters 2 X 2 X
DIFF Count Parameters 3 X 3 X
DIFF Percent Parameters 4 X 4 X
Comment 5 X 5 X
Definitive Flags 6 X
Suspect Flags 7 X
Conditional Flags 8 X
Other Flags 9 X
Flags 6 X
Demographics 7 X 10 X
DF1 DataPlot 8 X 11
DF2 DataPlot 9 X 12
DIFF (VCS) Histogram 10 X 13
RBC Histogram 11 X 14 X
PLT Histogram 12 X 15 X
RETICS Parameters 13 X 16 X
DF5 LS DataPlot 14 X 17
DF6 OP DataPlot 15 X 18
RETICS Histogram 16 X 19
WBC Threshold 26 X
WBC Threshold Full Histogram 27 X
DIFF Latex Parameters 31 X
RETIC Latex Parameters 32 X
Control Information 34 X
Control CBC Parameters 35 X
Control DIFF Count Parameters 36 X
Control DIFF Percent Parameters 37 X
Control RETICS Parameters 38 X
DIFF graphics 50 X
Retic graphics 51 X
X in 1G.1 + Retics column = Described under Heading 4.2, STKS 1G.1 + Retics Format.
X in LH 750 Workstation column = Described under Heading 4.3, LH 750 Workstation Format.
PN 4277303D 4-3
1G.1 + RETICS FORMAT
4.2 1G.1 + RETICS FORMAT

Message Structure
The Presentation deals with the high level format of the message. The data bytes of the
transmission blocks, when collected together, exhibit the following high level format.
PREAMBLE GROUP x1 • • • GROUP xn POSTAMBLE
Preamble
The preamble marks the beginning of a message.
C L C L C L C L C L C L C L
R F R F R F R F R F R F - - - - - - - - - - - - - - R F
Group
D FIELD C L FIELD C L • • • • FIELD C L

C COUNT R F 1 R F N R F
1
The DC1 (11H) character marks the beginning of a Group. There is no relationship between
the order in which the groups are defined in the following sections and the order in which
they appear after the preamble. Groups will never be omitted.
Each Group contains a DC1 (11H) character, marking the beginning of the section, a two
character field count and a variable number of fields each ending with a CR (0DH) and an LF
(0AH).
Note: When there are no data for a Group, the Group will appear as follows:
D C L
C 0 0 R F
1
Field Count
Each Group has a Field Count which identifies the number of fields contained within the
Group. Valid field counts can be from 0-255. The field count uses two characters to give the
ASCII representation of the hexadecimal value.
MS LS Two-byte ASCII representation of Hex

CHAR CHAR value between 00 and FF (0 to 255)
The field count will reflect the total number of fields transmitted within the Group. The field
count will always be the sum of CR/LF pairs within the Group minus one.
4-4 PN 4277303D
1G.1 + RETICS FORMAT 4
Fields
Each Group is made up of variable length fields which may or may not be padded with
SP (20H) characters. A field may have a variable length tag preceding the data separated with
one or more SP (20H) characters. The data portion of a field will only contain ASCII
characters in the range of 20H to 7EH inclusive. Chapter 6 tabulates the valid ASCII
characters. Fields are separated by a CR (0DH) and an LF (0AH) character.
Note: A field's position within a Group must not be used to identify it. Fields within a Group
may appear in any order or may be omitted. The CR/LF should be used to find a field and the
Tag used to identify it.
The syntax for the Data Format of a field is:
A - Alpha characters (a..z and A..Z and space) or (61H..7AH and 41H..5AH and 20H)
N - Numeric characters (0..9 and + - .) or (30H..39H and 2BH 2DH 2EH)
X - Printable characters (20H..7EH)
Sep - Separator one or more SP characters
SP Space (ASCII 20H). Please note that all field transmissions will
include a SP between the tag and data format (this will be implied
in the following sections), as illustrated below:
Tag Sep Data Format

T I M E N N : N N : N N
Postamble
D C L C L C L
C R F R F - - - - - - - - - - - - - - R F
1
The postamble marks the end of the current message. There are 16 groups defined in the
current implementation of the “STKS 1G.1 + Retics” format. Future revisions may include
additional groups. Any future additions will not disrupt the numbering of the groups as
presently defined.
PN 4277303D 4-5
Group Definition
Table 4.2 lists the groups supported by the 1G.1 + Retics format.
Table 4.2 Group Definition: 1G.1 + Retics
Group 1G.1 +Retics +

Number Group Name Subsets
1 General Information X
2 CBC Parameters X
3 DIFF Count Parameters X
4 DIFF Percent Parameters X
5 Comments X
6 Flags X
7 Demographics X
8 DF1 DataPlot *
9 DF2 DataPlot *
10 DIFF (VCS) Histogram *
11 RBC Histogram X
12 PLT Histogram X
13 Retic Parameter X
14 DF5 DataPlot *
15 DF6 DataPlot *
16 Retic Histogram *
X = Supported by the LH 750, STKS 1G.1 + Retics ” format.
* = Not supported the LH 750 “STKS 1G.1 + Retics ” format. The Group is transmitted with a
field count of 00.
General Information Group Fields

Table 4.3 identifies the field names, test types, and applicable format of General Information
Group transmissions.
Table 4.3 General Information -- Group 1
Field Name Test Type 1G.1 + Retics

Run Date C, CD, CDR, CR, R X
Run Time C, CD, CDR, CR, R X
ID #1 C, CD, CDR, CR, R X
ID #2 C, CD, CDR, CR, R X
Cassette/Position C, CD, CDR, CR, R X
Sequence Number C, CD, CDR, CR, R X
1E Cassette/Position C, CD, CDR, CR, R X
ID #1 Status C, CD, CDR, CR, R X
4-6 PN 4277303D
Cassette/Position Status C, CD, CDR, CR, R X

ToDo List Status C, CD, CDR, CR, R X
X = Supported by the LH 750, “STKS 1G.1 + Retics” format.
Run Date Field:
Tag Data Format Data Length Comments

D A T E N N / N N / N N 9 bytes Date output is
month/day/year, regardless
of the system date
configuration.
Run Time Field:

T I M E N N : N N : N N 12 bytes Time output is on 24 hours,
regardless of the system
time configuration. Time
ouput is
hours:minutes:seconds..
ID #1 Field:

I D 1 X X X • • • X X X X X 16 bytes SAMPLE ID
ID #2 Field:

I D 2 X X X • • • X X X X X 16 bytes Concatenation of Patient
last and first name
separated by a comma
and a space. Only the
first 16 characters of the
concatenated string are
transmitted.
Example: “Doe, John.”
PN 4277303D 4-7
Cassette/Position Field:

C A S S / P O S X X X X / X X 8 bytes CASS/POS output is
cassette
number/cassette
position.
‘SECONDA’ - Manual
sampling mode.
Sequence Number:

S E Q U E N C E X X X X X X 7 bytes Sequence Number
1E Cassette/Position Field:

1 E C / P A X X X X X X 8 bytes Date Values:
‘S’ - Manual sampling
mode
‘ ‘ - Automatic sampling
mode.
ID #1 Status Field:

I D 1 S T A T U S A 2 bytes Data Values:
‘P’ - positive ID.
‘E’ - edited ID.
‘ ‘ - not edited and not
positive ID.
‘N’ - Auto-numbered.
Precedence order is
‘E’, ‘N’, ‘P’ and ‘ ‘.
Cassette/Position Status Field:

C / P S T A T U S A 2 bytes Data Values:
‘P’ - positive ID.
‘E’ - edited ID.
‘ ‘ - not edited and not
positive ID.
Precedence order is: ‘E,
‘P’ and ‘ ‘.
4-8 PN 4277303D
ToDo List Status:

W L S T A T U S A A A A A A A A 9 bytes See note below.*
*Data Values:
‘GOOD’ = O.K. (Complete)
‘PART ASP’ = Partial aspiration
‘NO READ’ = Bar code not read
‘NO MATCH’ = Match not found in ToDo list
‘ ’ (20H) = Primary ID is cassette position and sample ID does not match preassigned ID
CBC Parameter Group Fields

Table 4.4 identifies the field names, test types, and applicable format of CBC Parameter
Group transmissions
Table 4.4 CBC Parameters -- Group 2

WBC C, CD, CDR, CR, Edited X
RBC C, CD, CDR, CR, R†, Edited X
HGB C, CD, CDR, CR, Edited X
HCT C, CD, CDR, CR, Edited X
MCV C, CD, CDR, CR, Edited X
MCH C, CD, CDR, CR, Edited X
MCHC C, CD, CDR, CR, Edited X
RDW C, CD, CDR, CR, Edited X
PLT C, CD, CDR, CR, Edited X
PCT C, CD, CDR, CR, Edited X
MPV C, CD, CDR, CR, Edited X
PDW C, CD, CDR, CR, Edited X
†For “Retic only” samples, only the RBC parameter is applicable. If the RBC parameter value is not
available, then it will not be transmitted and this group will have a field count of 00.
Each field in the CBC Parameters Group will have the following format:

A A A A X X X X X X A A A See “Data See Below.**
Format” column.
6 char numeric 3 char flag

data max. data max.
Result Format:**
PN 4277303D 4-9
The parameter result will be transmitted in the Reporting Units with the extra digit criteria
selected on the LH SeriesWorkstation.
Numeric Data: **
If the numeric data of each format (for example, xx.xx) does not contain a decimal number,
then it contains one of the following: Flag Data: **
Result Comments
..... Incomplete computation
+++++ Parameter overrange
----- Parameter voteout
The flag data characters for the CBC Parameter Group Fields are as follows in Table 4.5.
Table 4.5 lists all the possible flags generated on the LH Series Workstation and how they
translate to the STKS “1G.1 + Retics” format.
Table 4.5 Flag Data for CBC Parameter Group Fields
STKS 1G.1 Formats

Translation
Comments (3 characters)
Review parameter. R
Exceeds reference interval high limit H
Exceeds reference interval low limit L
Exceeds action high limit H
Exceeds action low limit L
Exceeds critical high limit H
Exceeds critical low limit L
Operator edited result E
Recalculated result from edited result e
MCV threshold failure * R
Delta check failed
Partial aspiration/blood detector disabled R
Result exceeds linearity
* = Not supported by the “STKS 1G.1 + Retics“ format.
DIFF Count Parameter Group Fields

Table 4.6 identifies the field names, test types, and applicable format of DIFF Count
Parameter Group transmissions.
Table 4.6 DIFF Count Parameters -- Group 3

LY# CD, CDR, Edited X
MO# CD, CDR, Edited X
NE# CD, CDR, Edited X
4-10 PN 4277303D
EO# CD, CDR, Edited X

BA# CD, CDR, Edited X
NRBC# CD, CDR, Edited
X = Supported by the “STKS 1G.1 + Retics ” format.
This group is applicable to “CBC/DIFF” and “CBC/DIFF/Retic” sample runs. For all other
sample runs, this group will have a field count of 00.
Each field in the DIFF Count Parameters Group will have the following format:

Format”
column.
data max. data max.
Result Format: **
selected on the LH Series Workstation.
Numeric Data: **
then it contains one of the following:
Result Comments
::::: Flow cell clogged (Full clog, Partial clog 1 or Partial clog 2)
????? Invalid data: could not calculate DIFF#.
+++++ WBC parameter overrange or sum of DIFF# parameters is greater than 500.0 x
106 cells/µL (whole blood) or sum of DIFF# parameters is greater than 600.0 x
106 cells/µL (predilute).
Flag Data: **
The flag data characters for the DIFF Count Parameter Group fields are as follows in
Table 4.7:
PN 4277303D 4-11
Table 4.7 Flag Data for Diff Count Parameter Group Fields
STKS 1G.1 Formats

Translation
Review parameter: WBC threshold failure R
or WBC exceeds linearity or sum of
DIFF% results exceeds 100.
Parameter value exceeds Delta check
limits
DIFF Percent Parameter Group Fields

Table 4.8 identifies the field names, test types, and applicable format of DIFF Percent
Parameter Group transmissions.
Table 4.8 DIFF Percent Parameters -- Group 4

LY% CD, CDR, Edited X
MO% CD, CDR, Edited X
NE% CD, CDR, Edited X
EO% CD, CDR, Edited X
BA% CD, CDR, Edited X
NRBC% CD, CDR, Edited
X = Supported by the “STKS 1G.1 + Retics” format.
This group is applicable to “CBC/DIFF” and “CBC/DIFF/Retics” sample runs. For all other
sample runs, this group will have a field count of 00.
4-12 PN 4277303D

Format”
column.
data max. data max.
Result Format:**
Numeric Data: **
Result Comments
+++++ Sum of DIFF% parameter results is not equal to 100% +0.1, or DIFF# parameters is
greater than 600.0 x 106 cells/µL (predilute).
Flag Data: **
The flag data characters for the DIFF Percent Parameter Group fields will be as follows in
Table 4.9:
Table 4.9 Flag Data for DIFF Percent Parameter Group Fields
STKS 1G.1 Formats

Translation
Review parameter R
Delta check failed
PN 4277303D 4-13

Comment Group Fields

Table 4.10 identifies the field names, test types, and applicable format of Comment Group
transmissions.
Table 4.10 Comment -- Group 5

Comment C, CD, CDR, CR, R X
X = Supported by the STKS 1G.1 + Retics ” format.
The Comment Group can have a variable number of fields which consist of a variable number
of bytes. Each Comment field is made up of an ASCII string identifying the appropriate flag.
Data Format Data Length Comments

X X X X X X X X • • • X X X X X X X 64 bytes
maximum
Flag Group Fields

Table 4.11 identifies the field names, test types, and applicable format of Flag Group
transmissions.
Table 4.11 Flag -- Group 6

Variable C, CD, CDR, CR, R X
Note: This group will always be transmitted and will only be sent once per sample
transmission.
The Flag Group can have a variable number of fields that consist of a variable number of
bytes. There are no Tags associated with a Flag field. Each Flag field is made up of an ASCII
string identifying the appropriate flag.
Data Format Data Length Comments

X X X X X X X X • • • X X X X X X X Variable
Table 4.12 lists all the possible flags generated on the LH Series Workstation and how they
translate to the “STKS 1G.1 + Retics” format. For example, a Low Event # suspect flag
4-14 PN 4277303D
message in LH 750 format is a Review message and an Abnormal WBC Pop message in “STKS
1G.1 + Retics ” format.
Table 4.12 LH 750 Workstation/STKS 1G.1 + Retics Messages
LH Series Workstation Message STKS 1G.1 + Retics
Suspect Flags
Low Event # Review Slide Abnormal WBC Pop
Verify Diff Review Slide Abnormal WBC Pop
Ne Blasts Blasts Abnormal WBC Pop
Ly Blasts Blasts Abnormal WBC Pop
Mo Blasts Blasts Abnormal WBC Pop
Imm. NE 1 Imm Grans/Bands 1 Abnormal WBC Pop
Imm. NE 2 Imm Grans/Bands 2 Abnormal WBC Pop
Variant LY Variant Lymphs Abnormal WBC Pop
NRBC NRBCs Abnormal RBC Pop
WBC Interference (N/A) (N/A)
RBC Interference (N/A) (N/A)
Dimorphic Reds Dimorphic RBC Pop Abnormal RBC Pop
Red Cell Agglutination RBC Agglutination Abnormal RBC Pop
Verify Retic (N/A) Verify Retic
Abnormal Retic Pattern (N/A) (N/A)
Platelet Clumps Platelet Clumps Abnormal PLT Pop
Giant Platelets Giant Platelets Abnormal PLT Pop
Inconsistent Diff CD (N/A) (N/A)
Abnormal Subsets Abnormal Subsets (N/A)
WBC Exceeds Linearity (N/A) (N/A)
Definitive Flags
Leukopenia Leukopenia Abnormal WBC Pop
Leukocytosis Leukocytosis Abnormal WBC Pop
Neutropenia % Neutropenia % Abnormal WBC Pop
Neutropenia # Neutropenia # Abnormal WBC Pop
Neutrophilia % Neutrophilia % Abnormal WBC Pop
Neutrophilia # Neutrophilia # Abnormal WBC Pop
Lymphopenia % Lymphopenia % Abnormal WBC Pop
Lymphopenia # Lymphopenia # Abnormal WBC Pop
Lymphocytosis % Lymphocytosis % Abnormal WBC Pop
Lymphocytosis # Lymphocytosis # Abnormal WBC Pop
Monocytosis % Monocytosis % Abnormal WBC Pop
PN 4277303D 4-15
Table 4.12 LH 750 Workstation/STKS 1G.1 + Retics Messages (Continued)

Monocytosis # Monocytosis # Abnormal WBC Pop
Eosinophilia % Eosinophilia % Abnormal WBC Pop
Eosinophilia # Eosinophilia # Abnormal WBC Pop
Basophilia % Basophilia % Abnormal WBC Pop
Basophilia # Basophilia # Abnormal WBC Pop
Anemia Anemia Abnormal RBC Pop
Anisocytosis Anisocytosis Abnormal RBC Pop
1+ Anisocytosis 1+ Anisocytosis Abnormal RBC Pop
Microcytosis Microcytosis Abnormal RBC Pop
1+ Microcytosis 1+ Microcytosis Abnormal RBC Pop
Macrocytosis Macrocytosis Abnormal RBC Pop
1+ Macrocytosis 1+ Macrocytosis Abnormal RBC Pop
Hypochromia Hypochromia Abnormal RBC Pop
1+ Hypochromia 1+ Hypochromia Abnormal RBC Pop
(N/A) 1+ Poikilocytosis Abnormal RBC Pop
Erythrocytosis Erythrocytosis Abnormal RBC Pop
Pancytopenia Pancytopenia Abnormal WBC Pop and
Abnormal RBC Pop and
Abnormal PLT Pop
Reticulocytosis (N/A) (N/A)
Thrombocytopenia Thrombocytopenia Abnormal PLT Pop
Thrombocytosis Thrombocytosis Abnormal PLT Pop
Small Platelets Small Platelets Abnormal PLT Pop
Large Platelets Large Platelets Abnormal PLT Pop
H&H Check Failed (N/A) (N/A)
4-16 PN 4277303D
Parameter Flags
..... ..... Abnormal WBC Pop and/or

Abnormal RBC Pop and/or
Abnormal PLT Pop and/or
Verify Retic
+++++ +++++ Abnormal WBC Pop and/or
Abnormal PLT Pop
----- ----- Abnormal WBC Pop and/or
Verify Retic
????? ????? Abnormal WBC Pop and/or
Verify Retic
::::: ::::: Abnormal WBC Pop and/or
Verify Retic
R R (N/A)
H H (N/A)
L L (N/A)
E E Edited Data
e e Edited Data
D (N/A) (N/A)
+ +++++ Abnormal WBC Pop and/or
Verify Retic
Other Flags
Edited Data Edited Data (N/A)
Preliminary Report Preliminary Report (N/A)
Additional Test Report (N/A) (N/A)
Incomplete Collation Collate Failed (N/A)
SYSTEM ALARM (N/A) (N/A)
PN 4277303D 4-17

Completed Report (N/A) (N/A)
Blood Detector Disabled (N/A) (N/A)
Sample validated (N/A) (N/A)
Sample not validated (N/A) (N/A)
AutoValidated (N/A) (N/A)
STKS Messages
PCT IS FOR RESEARCH ONLY, NOT FOR DIAGNOSTIC PROCEDURES
PDW IS FOR RESEARCH ONLY, NOT FOR DIAGNOSTIC PROCEDURES
Note: These flags will be produced only for the research parameters that are transmitted.
Demographics Group Fields

Table 4.13 identifies the field names, test types, and applicable format of Demographics
Table 4.13 Demographics -- Group 7

Date of Birth C, CD, CDR, CR, R X
User Field #1 C, CD, CDR, CR, R X
Sex C, CD, CDR, CR, R X
Location C, CD, CDR, CR, R X
Physician C, CD, CDR, CR, R X
User Date C, CD, CDR, CR, R X
User Time C, CD, CDR, CR, R X
Note: This group will be transmitted only once per sample transmission.
4-18 PN 4277303D
Date of Birth Field:

B I R T H N N / N N / N N N N 11 bytes Date of birth output
is month/day/year,
regardless of the
system date
configuration.
The Date Of Birth
Year is Always 4
Characters.
User Field #1:

U F 1 X X • • • X X X 17 bytes
User Field #2:

User Field #3:

Sex Field:

S E X A 2 bytes Data Value:
‘M’ Male
‘F’ Female
‘ ’ (20H) Unknown
Location Field:

L O C A T I O N X X X • • • X X X 17 bytes
PN 4277303D 4-19
Physician Field:

P H Y S I C I A N X X X • • • X X X 22 bytes Concatenation of
Physician last and
first name separated
by a comma and a
space. Only the first
22 characters of the
concatenated string
are transmitted.
Example: “Smith,
Peter.”
User Date Field:

U D A T E N N / N N / N N 9 bytes Sample Drawn Date.
Output format is
month/day/year,
regardless of the
system date
configuration.
User Time Field:

U T I M E N N : N N : N N 9 bytes Sample Drawn Time.
Output is on 24
hours, regardless of
the system time
configuration.
Time output is
hours:minutes:
seconds.
DF1 DataPlot Group Fields

The 1G.1 + Retics format does not support transmission of this group. This group will be
transmitted with field count of 00.
DF2 DataPlot Group Fields

DIFF (VCS) Histogram Group Fields

4-20 PN 4277303D
RBC Histogram Group Fields
Table 4.14 identifies the field names, test types, and applicable format of RBC Histogram
Group Field transmissions.
Table 4.14 RBC Histogram -- Group 11

RBC Histogram C, CD, CDR, CR X
If RBC histogram graphic transmission is disabled, this group is transmitted with a field of 00.
RBC Histogram Field:

R B C H H H H • • • H H H 512 bytes See Chapter 8 for
graphic
reconstruction.
PLT Histogram Group Fields

Table 4.15 identifies the field names, test types, and applicable format of PLT Histogram
Table 4.15 PLT Histogram -- Group 12

PLT Histogram C, CD, CDR, CR X
PLT Fit Histogram C, CD, CDR, CR X
If PLT histogram graphic transmission is disabled, this group is transmitted with a field of 00.
Note: When the PLT histogram graphic transmission is enabled the data in the PLTF field is
always sent. Ignore the data sent in the PLTF field if an R flag is present for the PLT parameter
sent in the CBC Parameter Group.
PLT Histogram Field:

P L T H H H H • • • H H H 256 bytes See Chapter 8 for
graphic
reconstruction.
PN 4277303D 4-21
PLT Fit Histogram Field:

P L T F H H H • • • H H H 512 bytes See Chapter 8 for
graphic
reconstruction.
RETIC Parameters Group Fields

Table 4.16 identifies the field names, test types, and applicable format of RETIC Parameters
Table 4.16 RETIC Parameters -- Group 13

Retic Run Date CDR, CR, R X
Retic Run Time CDR, CR, R X
Retic ID CDR, CR, R X
Retic Cassette Position CDR, CR, R X
RET% CDR, CR, R, Edited X
RET# CDR, CR, R, Edited X
MRV CDR, CR, R, Edited X
MI (now called IRF) CDR, CR, R, Edited X
X = Supported by the LH 750, STKS 1G.1 + Retics ” format.
Retic Run Date Field:

R E T D A T E N N / N N / N N 9 bytes Retic Run Date. Output is
month/day/year, regardless
of the system time
configuration.
Note: In the case of a
“CBC/Retic” or
“CBC/Diff/Retic” sample
run, the Date and Time of
the General Information
group are duplicated in the
RETDATE and RETTIME
fields.
4-22 PN 4277303D
Retic Run Time Field:

R E T T I M E N N : N N : N N 9 bytes Retic Run Time. Output is
on 24 hours, regardless of
the system time
configuration. Time output
is hours:minutes:seconds.
Note: In the case of a
“CBC/Retic” or
“CBC/Diff/Retic” sample
run, the Date and Time of
the General Information
group are duplicated in the
RETDATE and RETTIME
fields.
Retic ID Field:

R I D X X • • • X X X 17 bytes See Note**
**Note: In the case of a “CBC/Retic” or “CBC/DIFF/Retic” sample run, the Sample ID

transmitted in the ID1 field is duplicated in this field.
Retic Cassette/Position Field:

R C A S S / P O S X X X X / X X 8 bytes This field only applies
to the 1G.1+ Retics
host transmission
protocol.
Retic Parameter Results Field:

Format”
column.
data max. data max.
Result Format:**
1. The parameter result will be transmitted in the Reporting Units with the extra digit
criteria selected on the LH Series Workstation.
PN 4277303D 4-23
Numeric Data: **
Result Comments
????? Invalid data: could not calculate RET#.
+++++ Retic parameter result overrange
RET# > 999.9 x 106cells/µL
RET% > 100%
Flag Data: **
The flag data characters for the Retic Parameter Group fields will be as follows in Table 4.17:
Table 4.17 Flag Data for Retic Parameter Group Fields
LH 750 Workstation Comments STKS 1G.1 + Retics

Review parameter R
Delta check failed
If a Retic parameter is disabled, results for that parameter will NOT be transmitted. The IRF
parameter result is transmitted with the label MI for backward compatibility reasons.
DF5 LS DataPlot Group Fields

1G.1 + Retics format does not support transmission of this group. This group will be
DF6 OP DataPlot Group Fields

4-24 PN 4277303D
LH 750 WORKSTATION FORMAT 4
RETIC Histogram Group
4.3 LH 750 WORKSTATION FORMAT

Message Structure
The Presentation deals with the high level format of the message. The data bytes of the
transmission blocks, when collected together, exhibit the following high level format.
PREAMBLE TRANSMISSION IDENTIFICATION TEST a IDENTIFICATION GROUP x1 • • • • GROUP xn • • •

• • • •
• • • TESTz IDENTIFICATION GROUP x1 • • • • GROUP xn POSTAMBLE

• • • •
Preamble: The preamble marks the beginning of a message.
C L C L C L C L C L C L C L
R F R F R F R F R F R F - - - - - - - - - - - - - - R F
Transmission Identification:
S TRANSMISSION C L TRANSMISSION C L TRANSMISSION C L

TEST R F TEST R F OOO TEST R F
COUNT 1 N
The ASCII character "S" marks the beginning of the transmission.
TRANSMISSION TEST COUNT defines the number of tests that will be transmitted in this
particular transmission. Not all Test Types are sent in all transmissions.
Each Transmission Test is identified by the order in which it appears after the preamble. The
transmission test will never be transmitted out of order.
The Transmission Tests are up to 32 characters long. Table 4.18 lists the available
Transmission Tests and Test IDs by Test Type.
Table 4.18 Transmission Tests and Test IDs by Test Type
Test Type Transmission Tests Test IDs

C “CBC” “CBC”
CD “CBC”, “DIFF” “CBC”, “DIFF”
R “RETIC” “RETIC”
CR “CBC”, “RETIC” “CBC”, “RETIC”
CDR “CBC”, “DIFF”, “RETIC” “CBC”, “DIFF”, “RETIC”
C & CD Control “5CC” “CBC”, “DIFF”
R Control “RETICC” “RETIC”
CR Control “RETICC” “CBC”, “RETIC”
CDR Control “RETICC” “CBC”, “DIFF”, “RETIC”
CD Latex “DLATEX” “CBC”, “DIFF”
PN 4277303D 4-25
LH 750 WORKSTATION FORMAT
Table 4.18 Transmission Tests and Test IDs by Test Type

CDR Latex “LATEXC” “CBC”, “DIFF”, “RETIC”
Retic Latex “RLATEX” “RETIC”
Currently only manually entered data is transmitted.
Test Identification:
T TEST C L GROUP C L
ID R F COUNT R F
The ASCII character "T" marks the beginning of a test identification.
TEST ID, see Table 4.18.
GROUP COUNT defines the number of groups of data in this particular TEST Identification.
Group:
G GROUP C L FIELD C L FIELD C L FIELD C L

NUMBER R F COUNT R F 1 R F • • • • N R F
The ASCII character "G" marks the beginning of a group. A group follows the test
identification fields.
GROUP NUMBER defines the group number. See Group Definition.
FIELD COUNT defines the number of fields in a particular group. See Field Count.
FIELD defines a field within a group. See Fields.
Note: When there is no data for a Group, the Group will not be sent.
Test Type Count:

The transmission identification section has a Test Type Count that identifies the number of
tests contained within the transmission. Valid test type count can be from 0-255. The test type
count uses two characters to give the ASCII representation of the hexadecimal value.

Group Count:
Each test identification has a Group Count that identifies the number of groups contained
within the test. Valid group counts can be from 0-255. The group count uses two characters
to give the ASCII representation of the hexadecimal value.

Group Number:
Each group identifier has a Group Number that identifies the group number contained within
4-26 PN 4277303D
the group identifier. Valid group number can be from 0-255. The group number uses two
characters to give the ASCII representation of the hexadecimal value.

Field Count:
Each Group has a Field Count that identifies the number of fields contained within the
Group. Valid field counts can be from 0-255. The field count uses two characters to give the
ASCII representation of the hexadecimal value.

Fields:
Each Group is made up of variable length fields which may or may not be padded with SP
(20H), or HT (09H) characters. A field may have a variable length tag preceding the data,
separated by one or more SP (20H), or HT (09H) characters. Please note that all field
transmissions will include a SP between the tag and data format (this will be implied in the
following sections), as illustrated below:
Tag Sep Data Format

T I M E N N : N N : N N
The data portion of a field will only contain ASCII characters in the range of 20H to 7EH
inclusive. Heading 6.2, VALID HOST COMMUNICATIONS ASCII CODES identifies the valid
ASCII characters. Fields are separated with a CR (0DH) and an LF (0AH) character.
Note: A field's position within a Group must not be used to identify it. Fields within a Group
may appear in any order or may be omitted. The CR/LF should be used to find fields and the
Tag used to identify it.
ALL FIELDS ARE OF MAXIMUM 32 CHARACTERS LONG UNLESS OTHERWISE NOTED.
The following abbreviations are used to describe the fields:
SEP - one or more SP (20H) or HT (09H) characters.
The syntax for the Data Format of a field is:
A - Alpha characters (a..z and A..Z and space) or (61H..7AH and 41H..5AH and 20H)
N - Numeric characters (0..9 and + - .) or (30H..39H and 2BH 2DH 2EH)
X - Printable characters (20H..7EH)
H - Hexadecimal (0..9 and A..F)
characters
O - Other characters (CR and LF) or (0DH and 0AH)
PN 4277303D 4-27
Postamble:
C L C L C L
R F R F - - - - - - - - - - - - - - R F
The postamble marks the end of the current message.
There are 24 groups defined in the current implementation of the LH 750 Workstation
Format. Future revisions of LH Series Workstation may include additional groups. Any future
additions will not disrupt the order of the groups as currently defined.
Group Definition
The abbreviations used to identify tests the group applies to are:
C CBC
D DIFF
R RETICS
Table 4-19 lists the groups transmitted under 2A, 3A, GEN•S, and LH 750 Workstation
formats.
Table 4.19 Transmitted Groups
Group Number Group Name Tests LH 750 Workstation

1 General Information C D R X, *
2 CBC Parameters C X
3 DIFF Count Parameters D X
4 DIFF Percent Parameters D X
5 Comments C D R X, *
6 Definitive Flags C D R X, *
7 Suspect Flags C D R X, *
8 Conditional Flags C D R X, *
9 Other Flags C D R X, *
10 Demographics C D R X, *
14 RBC Histograms C X
15 PLT Histograms C X
16 RETIC Parameters R X
26 WBC Threshold C X
27 WBC Threshold Full Histogram C X
31 DIFF Latex Parameters D X
32 Retic Latex Parameters R X
34 Control Information C D R X
35 Control CBC Parameters C X
36 Control DIFF Count Parameters D X
37 Control DIFF Percent Parameters D X
4-28 PN 4277303D
Table 4.19 Transmitted Groups
Group Number Group Name Tests LH 750 Workstation

38 Control Retics Parameters R X
50 DIFF Graphics D X
51 Retic Graphics R X
X = Supported by the LH 750, Workstation format.
* = Indicates that the group information will not be repeated with every test.
Details of Group fields are defined in the following sections.
General Information Group Fields

Table 4.20 identifies the field names, test types, and applicable format of General Information
transmissions.
Field Name Test Type LH 750 Workstation

Run Date C, CD, CDR, CR, R X
Run Time C, CD, CDR, CR, R X
Sample Age C, CD, CDR, CR, R X
Sample ID C, CD, CDR, CR, R X
Pre Assigned Sample ID C, CD, CDR, CR, R X
Cassette / Position C, CD, CDR, CR, R X
Pre Assigned Cassette / Position C, CD, CDR, CR, R X
Sample ID Status C, CD, CDR, CR, R X
Cassette / Position Status C, CD, CDR, CR, R X
Sample Status C, CD, CDR, CR, R X
Instrument ID C, CD, CDR, CR, R X
Operator ID C, CD, CDR, CR, R X
Dilution Factor C ~X
Body Fluid C ~X
X = Supported by the LH 750, Workstation format.
~ = New for the LH 750 Workstation format.
PN 4277303D 4-29
Run Date Field:

D A T E N N / N N / N N 8 bytes Run Date.
Output is
month/day/year,
regardless of the
system date
configuration.
Run Time Field:

T I M E N N : N N : N N 8 bytes Run Time. Output is on
24 hours, regardless of
the system time
configuration. Time
output is
hours:minutes:seconds.
Sample Age Field:

S A N N N N : N N : N N 11 bytes Age of sample at run
time. Format is DD
HH:MM:SS. Computed
from Draw Date and
Draw Time. This field
will not appear if draw
date and time are invalid
or after run date and
time.
Sample ID Field:

I D 1 X X X • • • X X X X X 16 bytes Bar code read on tube
by instrument at
aspiration time, entered
on the Diluter keypad
for Manual mode
aspiration, or ID
generated by
Auto-numbering
feature.
4-30 PN 4277303D
Pre Assigned Sample ID Field:

P I D 1 X X X • • • X X X 16 bytes PRE ASSIGNED
SAMPLE ID

C A S S P O S A C C C C P P 7 bytes CASS/POS output is
cassette number (‘C’)/
cassette position (‘P’).
Data values for 'A' are:
‘A’ for Automatic.
‘M’ for Manual.
Pre-Assigned Cassette/Position Field:

P C A S S P O S C C C C P P 6 bytes CASS/POS entered
through ToDo list.
Format is cassette
number (‘C’)/ cassette
position (‘P’). The
sample mode is not
specified at
preassignment time.
Sample ID Status Field:

I D 1 S T A T U S A 1 byte Data Values:
‘P’ - positive ID
‘E’ - edited ID
‘N’ - Auto-numbered
ID
‘ ’ (20H) - not edited,
not auto-numbered and
not part of positive
identification scheme.
Precedence order is: ‘E’,
‘N’, ‘P’ and ‘ ’ (20H).
PN 4277303D 4-31
Cassette/Position Status Field:

C / P S T A T U S A 1 byte Data Values:
‘P’ - positive ID
‘E’ - edited ID
‘ ’ (20H) - not edited and
not part of positive
identification scheme.
Precedence order is: ‘E’,
‘P’ and ‘ ’ (20H).
Sample Status:

S A S T A T U S X X • • • X X X 8 bytes See Table 4.21.
Instrument ID:

I N S T A A A • • • A A 16 bytes Identifies the LH 750
instrument this sample
was run on.
Operator ID:

O P R A A A • • • A A 14 bytes Identifies the user
logged on the LH 750
Workstation at the time
this sample run was
analyzed.
Dilution Factor::

D F A C T N N N N N 5 bytes Identifies amount by
volume by which
sample is diluted.This
field is always
transmitted. All have the
default value of 1.0
when used. The factor is
applied to transmitted
parameter values.
4-32 PN 4277303D
Body Fluid::

B O D F A A A • • A A A 16 bytes This field is only
transmitted when the
sample is a body fluid
and then only if “Enable
Body Fluid Tag” in
System Setup -
(Communication) is
checked. Current data is
always “Body Fluid”.
The parameters
displayed are WBC, and
RBC. (For LH 750
ONLY)
Table 4.21 Possible Values for Sample Status
Possible Value LH 750 Workstation Comments

PART ASP X Partial aspiration
NO READ X Bar code not read
NO MATCH X Match not found in ToDo list
MISMATCH X Non positive ID mismatch
INC COL X Incomplete collation
COMPLETE X No ToDo List errors
X in the LH 750 Workstation column = Supported by the LH 750 Workstation format.
CBC Parameters Group Fields

Table 4.22 identifies the field names, test types, and applicable format of CBC Parameter
transmissions.
Table 4.22 CBC Parameters -- Group 2

WBC C, CD, CDR, CR, Edited X
RBC C, CD, CDR, CR, Edited X
HGB C, CD, CDR, CR, Edited X
HCT C, CD, CDR, CR, Edited X
MCV C, CD, CDR, CR, Edited X
MCH C, CD, CDR, CR, Edited X
MCHC C, CD, CDR, CR, Edited X
RDW C, CD, CDR, CR, Edited X
PLT C, CD, CDR, CR, Edited X
PCT C, CD, CDR, CR, Edited X
MPV C, CD, CDR, CR, Edited X
PDW C, CD, CDR, CR, Edited X
PN 4277303D 4-33
Table 4.22 CBC Parameters -- Group 2 (Continued)

X = Supported by the LH 750 Workstation format.
Each field in the CBC Parameters Group will have the following format:

A A A • • A X X ••• X X A A A A See “Data See Below.**
Format”
column.
data max. data max.
1 char separator min.
Result Format: **
Numeric Data: **
Result Comments
+++++ Parameter overrange
----- Parameter voteout
Flag Data: **
The flag data characters for the CBC Parameter Group Fields are as follows in Table 4.23:
LH 750
Workstation Flags
4 (characters) Comments
R Review parameter
H Exceeds reference interval high limit
L Exceeds reference interval low limit
a H Exceeds action high limit
a L Exceeds action low limit
c H Exceeds critical high limit
c L Exceeds critical low limit
E Operator edited result
4-34 PN 4277303D
LH 750
Workstation Flags
4 (characters) Comments
e Recalculated result from edited result
* MCV threshold failure
D Delta check failed
P Partial aspiration/blood detector disabled
+ Result exceeds linearity
V Single Aperture Voteout (LH 500 Only)
v Result calculated from Single Aperture Voteout
DIFF Count Parameters Group Fields

Table 4.24 identifies the field names, test types, and applicable format of DIFF Count
Parameter transmissions.

LY# CD, CDR, Edited X
MO# CD, CDR, Edited X
NE# CD, CDR, Edited X
EO# CD, CDR, Edited X
BA# CD, CDR, Edited X
NRBC# CD, CDR, Edited X
Note: NRBC% will only be present if the NRBC Suspect Flag was triggered.

Format”
column.
data max. data max.
Result Format: **
PN 4277303D 4-35
Numeric Data: **
Result Comments
+++++ WBC parameter overrange or sum of DIFF# parameters is greater than 500.0 x
106 cells/µL (whole blood) or sum of DIFF# parameters is greater than 600.0 x
106 cells/µL (diluted).
Flag Data: **
The flag data characters for the DIFF Count Parameter Group fields are as follows in
Table 4.25:
Table 4.25 Flag Data for DIFF Count Parameter Group Fields
LH 750
Workstation Flags
(4 characters) Comments
R Review parameter: WBC threshold failure or WBC exceeds linearity or sum
of DIFF% results exceeds 100.
D Parameter value exceeds Delta check limits
v Result calculated from Single Aperture Voteout (For LH 500 ONLY)
4-36 PN 4277303D
DIFF Percent Parameters Group Fields
Table 4.26 identifies the field names, test types, and applicable format of DIFF Percent
Parameter transmissions.
Table 4.26 DIFF Percent Parameters -- Group 4
Field Name Test Type GEN•S Workstation

LY% CD, CDR, Edited X
MO% CD, CDR, Edited X
NE% CD, CDR, Edited X
EO% CD, CDR, Edited X
BA% CD, CDR, Edited X
NRBC% CD, CDR, Edited X
Each field in the DIFF Percent Parameters Group will have the following format:

Format”
column.
data max. data max.
Result Format: **
Numeric Data: **
Result Comments
+++++ Sum of DIFF% parameter results is not equal to 100% +0.1, or DIFF# parameters is
greater than 600.0 x 106 cells/µL (predilute).
Flag Data: **
The flag data characters for the DIFF Percent Parameter Group fields will be as follows in
Table 4.27:
PN 4277303D 4-37
LH 750
Workstation Flags
R Review parameter
Comment Group Fields

Table 4.28 identifies the field names, test types, and applicable format of Comment Group
Field transmissions.
Table 4.28 Comment -- Group 5

Comment C, CD, CDR, CR, R X
The Comment Group can have a variable number of fields which consist of a variable number
of bytes. Each Comment field is made up of an ASCII string identifying the appropriate flag.
When WBC correction is performed, the Uncorrected WBC will be printed at the beginning
of the Comments field as UWBC=XXXX. For non-English applications, the UWBC will be
translated into the language being used. (For LH 750 ONLY)

C O M M E N T X X X • • • X X X 254 bytes STKS format is only
64 characters long.
4-38 PN 4277303D
Definitive Flags Group Fields
Table 4.29 identifies the field names, test types, and applicable format of Definitive Flag
transmissions.
Table 4.29 Definitive Flags -- Group 6

Definitive Flag C, CD, CDR, CR, R X
transmission.

D E F I N I T X X X • • • X X X Up to 128 See Below.**
bytes
** The possible definitive flags generated on the LH Series Workstation are:
LH 750 Workstation Messages

Leukopenia
Leukocytosis
Neutropenia %
Neutropenia #
Neutrophilia %
Neutrophilia #
Lymphopenia %
Lymphopenia #
Lymphocytosis %
Lymphocytosis #
Monocytosis %
Monocytosis #
Eosinophilia %
Eosinophilia #
Basophilia %
Basophilia #
Anemia
Anisocytosis
1+ Anisocytosis
2+ Anisocytosis
3+ Anisocytosis
Microcytosis
1+ Microcytosis
2+ Microcytosis
3+ Microcytosis
Macrocytosis
1+ Macrocytosis
2+ Macrocytosis
3+ Macrocytosis
PN 4277303D 4-39
(Continued) LH 750 Workstation Messages (Continued)

Hypochromia
1+ Hypochromia
2+ Hypochromia
3+ Hypochromia
Erythrocytosis
Pancytopenia
Reticulocytosis
Thrombocytopenia
Thrombocytosis
Small Platelets
Large Platelets
H & H Check Failed
Suspect Flags Group Fields

Table 4.30 identifies the field names, test types, and applicable format of Suspect Flag
transmissions.
Table 4.30 Suspect Flags -- Group 7

Suspect Flag C, CD, CDR, CR, R X
transmission.
Note: When the LH System generates a “Cellular Interference” suspect message, the Host
transmission will replace the message with “WBC Interference”.

S U S P E C T X X X • • • X X X Up to 128 See Below.**
bytes
** The possible Suspect flags generated on the LH Series Workstation are:

Low Event #
Verify Diff
Ne Blasts
Ly Blasts
Mo Blasts
Imm NE 1
Imm NE 2
Variant LY
NRBC
Dimorphic Reds
Red Cell Agglutination
Verify Retic
4-40 PN 4277303D
LH 750 Workstation Messages (Continued)
Abnormal Retic Pattern
Platelet Clumps
Giant Platelets
WBC Interference
RBC Interference
WBC Exceeds Linearity
Conditional Flags Group Fields

Table 4.31 identifies the field names, test types, and applicable format of Conditional Flag
transmissions.
Table 4.31 Conditional Flags -- Group 8

Conditional Flag C, CD, CDR, CR, R X
Note: This group will be transmitted with the first test only in each sample transmission.

C O N D I T I O N X X X • • • X X X Up to 128 See Below.**
bytes
**The possible Conditional flags generated on the LH Series Workstation are:

Edited Data
Preliminary Report
Additional Test Report
Incomplete Collation
SYSTEM ALARM
Completed Report
Blood Detector Disabled
Sample validated
Sample not validated
AutoValidated
AutoValidation:
The auto validation flags show the validation status of test requests for a sample on printouts
and within the results transmission. A functional example of the validation codes is
described below.
A specimen is run in CDR mode with a CDR report format requested. Suppose
results are acceptable for CBC and Retic, but not for the Diff (e.g., a Decision
Rule fails, such as “If Blast [suspect flag], Perform Manual Diff”).
PN 4277303D 4-41
Note: The configuration of the Workstation affects the validation messages and
codes.
The CBC and Retic test groups are AUTOVALIDATED, but the Diff group is NOT
VALIDATED. There are two ways to indicate this. A conditional text message
SAMPLE NOT VALIDATED would be transmitted. Additionally, validation
codes C AV, R AV, D NV indicate the status of the individual test groups. The Diff
can later be validated manually, resulting in
SAMPLE VALIDATED, C AV, R AV, D V.
It is possible through use of the report format to have validation messages based on the report
profile (report format). For example, using the scenario above, if a CBC report profile was
requested, validation would be based solely on the results of the CBC group. For a complete
explanation, refer to the LH Series Workstation on-line help.
Validation Flags
AV AutoValidated
NV Not Validated
V Validated (manually)
Validation Codes
C CBC parameter block
D Diff parameter block
H H&H parameter block
R Retic parameter block
W WBC/Hgb/Plt parameter block
One of the conditional text messages below will always be transmitted.
r Sample not validated

r Sample validated
r AutoValidated
If “Enable Validation Codes” in Sytem Setup - [Communications] is checked (as described in
Heading 3.16, ENABLE VALIDATION CODES), the individual test group validation status
will be transmitted based on the profile selected and test mode run. The following columns
display the possible codes transmitted for the individual groups:
Sample Not Validated Sample Validated AutoValidated

C NV CV C AV
D NV DV D AV
R NV RV R AV
H NV HV H AV
W NV WV W AV
4-42 PN 4277303D
Other Flags Group Fields
Table 4.32 identifies the field names, test types, and applicable format of Other Flag
transmissions.
Table 4.32 Other Flags -- Group 9

Other Flag C, CD, CDR, CR, R X
Note: This group will be transmitted only once in each sample transmission.

O T H E R X X X • • • X X X Up to 128 See Below.**
bytes
**The other possible flags generated on the LH Series Workstation are:

HLR% IS FOR RESEARCH ONLY, NOT FOR DIAGNOSTIC PROCEDURES
HLR# IS FOR RESEARCH ONLY, NOT FOR DIAGNOSTIC PROCEDURES
PCT IS FOR RESEARCH ONLY, NOT FOR DIAGNOSTIC PROCEDURES
PDW IS FOR RESEARCH ONLY, NOT FOR DIAGNOSTIC PROCEDURES
MSCV IS FOR RESEARCH ONLY, NOT FOR DIAGNOSTIC PROCEDURES
Note: These flags will be produced only for the research parameters that are transmitted.
Demographics
Table 4.33 identifies the field names, test types, and applicable format of Demographics
Table 4.33 Demographics -- Group 10

Date of Birth C, CD, CDR, CR, R X
Sex C, CD, CDR, CR, R X
Location C, CD, CDR, CR, R X
Physician C, CD, CDR, CR, R X
Sample Drawn Date C, CD, CDR, CR, R X
Sample Drawn Time C, CD, CDR, CR, R X
Patient Identification C, CD, CDR, CR, R X
Patient Last Name C, CD, CDR, CR, R X
Patient First Name C, CD, CDR, CR, R X
PN 4277303D 4-43
Table 4.33 Demographics -- Group 10 (Continued)

Sequence Number C, CD, CDR, CR, R X
Profile C, CD, CDR, CR, R X
Number of slides made C, CD, CDR, CR, R X
Number of slides requested C, CD, CDR, CR, R X
Report Profile C, CD, CDR, CR, R X
Date of Birth Field:

B I R T H N N / N N / N N N N 10 bytes Date of Birth
Output is
month/day/year,
regardless of the
system date
configuration.
The Date Of Birth
Year is Always 4
Characters.
User Field #1:

U F 1 X X X • • • X X X 16 bytes
User Field #2:

User Field #3:

4-44 PN 4277303D
Sex Field:

S E X A 1 byte Data Value:
‘M’ Male
‘F’ Female
‘U’ Unknown
Location Field:

L O C A T I O N X X X • • • X X X 16 bytes
Physician Field:

P H Y S I C I A N X X X • • • X X X Up to 22 bytes Concatenation of
Physician’s last and
first name separated
by a comma and a
space. Example:
“Smith, Peter.”
Sample Drawn Date Field:

D D N N / N N / N N 8 bytes Sample Drawn Date.
Output is
month/day/year,
regardless of the
system date
configuration.
Sample Drawn Time Field:

D T N N : N N : N N 8 bytes Sample Drawn Time.
Output is on 24
hours, regardless of
the system time
configuration.
Time output is
hours:minutes
:seconds.
PN 4277303D 4-45
Patient Identification:

P I D X X X • • • X X X 16 bytes
Patient Last Name:

P L N X X X • • • X X X 16 bytes
Patient First Name:

P F N X X X • • • X X X 16 bytes
Sequence Number:

S E Q U E N C E X X X X X X 6 bytes
Number of Slides Made:

S M N 1 byte Indicates how many
slides were made for
that sample on the
LH 750 SlideMaker.
Possible values are: 0 or
1. If 0, this field is not
sent.
Number of Slides Requested:

S R N 1 byte Indicates how many
slides were requested
through preassignment
in the ToDo list for that
sample. Possible values
are: 0 or 1.
4-46 PN 4277303D
Report Profile Field:

R P N N 2 bytes Report Profile possible
values are from “01" to
"10". Always
transmitted.
Profile Selection:
Data transmission reflects profile selection. Parameters not in the selected

profile will not be transmitted. Test blocks (e.g., CBC, DIFF, etc.) which are not
in the current profile will not be transmitted. If a complete test block is omitted
from the current profile, the General Information Block (for sample
identification) will still be sent.
RBC Histogram Group Fields

Table 4.34 identifies the field names, test types, and applicable format of RBC Histogram
Table 4.34 RBC Histogram -- Group 14

RBC Histogram C, CD, CDR, CR X
If CBC histograms graphic transmission is disabled, this group will NOT be transmitted.
RBC Histogram Field:

R B C H H H H • • • H H H 512 bytes See Chapter 8 for
graphic
construction.
PLT Histogram Group Fields

Table 4.35 identifies the field names, test types, and applicable format of PLT Histogram
Table 4.35 PLT Histogram -- Group 15

PLT Histogram C, CD, CDR, CR X
PLT Fit Histogram C, CD, CDR, CR X
PN 4277303D 4-47
PLT Histogram Field:

P L T H H H H • • • H H H 256 bytes See Chapter 8 for
graphic
construction.
Note: When the PLT histogram graphic transmission is enabled the data in the PLTF field is
always sent. Ignore the data sent in the PLTF field if an R flag is present for the PLT parameter
sent in the CBC Parameter Group.
PLT Fit Histogram Field:

P L T F H H H • • • H H H 512 bytes See Chapter 8 for
graphic
construction.
RETIC Parameters Group Fields

Table 4.36 identifies the field names, test types, and applicable format of RETIC Parameters
Table 4.36 RETIC Parameters -- Group 16

RET% CDR, CR, R, Edited X
RET# CDR, CR, R, Edited X
MRV CDR, CR, R, Edited X
MSCV CDR, CR, R, Edited X
IRF CDR, CR, R, Edited X
HLR% CDR, CR, R, Edited X
HLR# CDR, CR, R, Edited X

Format”
column.
data max. data max.
Result Format: **
4-48 PN 4277303D
Numeric Data: **
Result Comments
RET# > 999.9 x 106 cells/µL
RET% > 100%
Flag Data: **
The flag data characters for the Retic Parameter Group fields will be as follows in Table 4.37:
Table 4.37 Flag Data for Retic Parameter Group Fields
LH 750
Workstation Flags
R Review parameter
v Result calculated from Single Aperture Voteout (For LH 500 ONLY)
MRV, MSCV, HLR%, HLR# and IRF results will be transmitted only if they have been enabled.
If a Retic parameter is disabled, results for that parameter will NOT be transmitted.
The LH Series Workstation does not support transmission of MI and OTHER fields. These
fields will NOT be transmitted.
PN 4277303D 4-49
WBC Threshold Group Fields

Table 4.38 identifies the field names, test types, and applicable format of the WBC Threshold
Monitor Group Field transmissions.
Table 4.38 WBC Threshold Monitor Group 26
Field Name Test Type LH 750S Workstation

WBC Threshold Monitor C, CD, CDR, CR X
WBC Threshold Monitor Field:

W B C T H H H • • • H H H 112 bytes See Chapter 8 for
graphic
construction.
WBC Threshold Full Histogram Group Fields

This group has the same description as WBC Threshold Group Fields with the exception that
additional data elements are added.
Table 4.38.1 WBC Full Histogram Monitor Group 27
Field Name Test Type LH 750S Workstation

WBC Threshold Monitor C, CD, CDR, CR X
WBC Threshold Full Monitor Field:

W B C F H H H • • • H H H 512 bytes See Chapter 8 for
graphic
construction.
DIFF Latex Parameters Group Fields

Table 4.39 identifies the field names, test types, and applicable format of DIFF Latex
Parameters Group transmissions.
Table 4.39 DIFF Latex Parameters -- Group 31

PRIMER F55, F57 X
V_MN F55, F57 X
C_MN F55, F57 X
S_MN F55, F57 X
4-50 PN 4277303D
Table 4.39 DIFF Latex Parameters -- Group 31
V_CV F55, F57 X

C_CV F55, F57 X
S_CV F55, F57 X
Each field in the DIFF Latex Parameters Group will have the following format:

A A A • • A X X ••• X X A See “Data See Below.**
Format”
column.
data max. data max.
Result Format: **
Numeric Data: **
Result Comments
Flag Data: **
The flag data characters for the DIFF Latex Parameter Group fields will be as follows as in
Table 4.40:
Table 4.40 Flag Data for DIFF Latex Parameters Group Fields
LH 750 Workstation
Flags (1 character) Comments
H Greater than expected value range.
L Less than expected value range.
PN 4277303D 4-51
RETIC Latex Parameters Group Fields

Table 4.41 identifies the field names, test types, and applicable format of RETIC Latex
Table 4.41 RETIC Latex Parameters -- Group 32
LH 750
Field Name Test Type Workstation
PRIMER F56, F57 X
V_MN F56, F57 X
C_MN F56, F57 X
S_MN F56, F57 X
V_CV F56, F57 X
C_CV F56, F57, X
S_CV F56, F57 X
Each field in the RETIC Latex Parameters Group will have the following format:

A A A • • A X X ••• X X A See “Data See Below.**
Format”
column.
data max. data max.
Result Format: **
Numeric Data:**
Result Comments
Flag Data: **
4-52 PN 4277303D
The flag data characters for the RETIC Latex Parameter Group fields will be as follows as in
Table 4.42:
Table 4.42 Flag Data for RETIC Latex Parameters Group Fields
LH 750 Workstation Flags

(1 character) Comments
H Greater than expected value range.
L Less than expected value range.
Table 4.43 identifies the field names, test types, and applicable format of Control Information
Table 4.43 Control Information -- Group 34

Run number C, CD, CDR, CR, R, LATEX *
Lot number C, CD, CDR, CR, R, LATEX X
User Control Name C, CD, CDR, CR, R, LATEX X
Expiration Date C, CD, CDR, CR, R, LATEX X
Run Date C, CD, CDR, CR, R, LATEX X
Run Time C, CD, CDR, CR, R, LATEX X
Operator C, CD, CDR, CR, R, LATEX X
IQAP ID C, CD, CDR, CR, R, LATEX X
Delete Flag C, CD, CDR, CR, R, LATEX X
Shift C, CD, CDR, CR, R, LATEX X
Cassette / Position C, CD, CDR, CR, R, LATEX X
Reference RBC Count R X
* = Not supported by the LH 750 Workstation format.
Run Number Field:
The LH Series Workstation does not support transmission of this field. This field will NOT be
transmitted.
Lot Number Field:

L O T # N N • • • N N 16 bytes
User Control Name Field:
PN 4277303D 4-53
U C N A M E X X X X X • • • X X 12 bytes
Expiration Date Field:

E X P D A T E N N / N N / N N 8 bytes Expiration Date.
is month/day/year,
regardless of the
system date
configuration.
Run Date Field:

D A T E N N / N N / N N 8 bytes Run Date.
Output is
month/day/year,
regardless of the
system date
configuration.
Run Time Field:

T I M E N N : N N : N N 8 bytes Run Time. Output is
on 24 hours,
regardless of the
system time
configuration.
Time output is
hours:minutes:
seconds
Operator Field:

O P E R A T O R X X • • • X 14 bytes
IQAP ID Field:

I Q A P X X X • • • X 12 bytes
4-54 PN 4277303D
Delete Flag Field:

D F L A G X 1 byte Run was excluded
from statistical
calculation on review
screen.
’Y’ = Run excluded.
SP = Run not
excluded.
Shift Field:

S H I F T X 1 byte Possible values are
0, 1, 2, or 3.
Reference the Online
Help for more
information.

C A S S P O S A C C C C P P 7 bytes CASS/POS output is
cassette number
(‘C’) / cassette
position (‘P’).
Data values for ‘A’
are:
‘A’ - Automatic
sampling
‘M’ - Manual
sampling
Reference RBC Count Field:

R R B C X X X X X 5 characters
numeric data
max.
Reference WBC Count Field:

R W B C X X X X X 5 characters
numeric data
max.
PN 4277303D 4-55
Control CBC Parameters Group Fields

Table 4.44 identifies the field names, test types, and applicable format of Control CBC
Table 4.44 Control CBC Parameters -- Group 35

WBC C, CD, CDR, CR X
RBC C, CD, CDR, CR X
HGB C, CD, CDR, CR X
HCT C, CD, CDR, CR X
MCV C, CD, CDR, CR X
MCH C, CD, CDR, CR X
MCHC C, CD, CDR, CR X
RDW C, CD, CDR, CR X
PLT C, CD, CDR, CR X
PCT C, CD, CDR, CR X
MPV C, CD, CDR, CR X
PDW C, CD, CDR, CR X
Each field in the Control CBC Parameters Group will have the following format:

Format”
column.
data max. data max.
Result Format: **
Numeric Data:**
Result Comments
----- Total voteout
+++++ Overrange
4-56 PN 4277303D
Flag Data: **
The flag data characters for the Control CBC Parameter Group fields will be as follows as in
Table 4.45:
Table 4.45 Flag Data for Control CBC Parameters Group Fields
LH 750
Workstation Flags
R Review parameter.
H Exceeds expected range high limit
L Exceeds expected range low limit
* MCV threshold failure
Control DIFF Count Parameters Group Fields

Table 4.46 identifies the field names, test types, and applicable format of Control DIFF Count
Table 4.46 Control DIFF Count Parameters -- Group 36

LY# CD, CDR X
MO# CD, CDR X
NE# CD, CDR X
EO# CD, CDR X
BA# CD, CDR X
NRBC# CD, CDR
Each field in the Control DIFF count Parameters Group will have the following format:

Format”
column.
data max. data max.
Note: NRBC# will only be present if the NRBC Suspect Message was triggered.
Result Format: **
PN 4277303D 4-57
Numeric Data:**
Result Comments

Flag Data: **
The flag data characters for the DIFF Count Parameter Group fields will be as follows in
Table 4.47:
Table 4.47 Flag Data for DIFF Count Parameter Group Fields
LH 750 Workstation
Flags (4 characters) Comments
R Review parameter.
Control DIFF Percent Parameters Group Fields

Table 4.48 identifies the field names, test types, and applicable format of Control DIFF
Percent Parameters Group transmissions.
Table 4.48 Control DIFF Percent Parameters -- Group 37

LY% CD, CDR X
MO% CD, CDR X
NE% CD, CDR X
EO% CD, CDR X
BA% CD, CDR X
NRBC% CD, CDR
4-58 PN 4277303D
Each field in the Control DIFF Percent Parameters Group will have the following format:

Format”
column.
data max. data max.
Result Format: **
Numeric Data:**
Result Comments
+++++ Sum of DIFF% parameter results not equal to 100% +0.1
Flag Data: **
The flag data characters for the DIFF percent Parameter Group fields will be as follows in
Table 4.49:
Table 4.49 Flag Data for Diff Percent Parameter Group Fields
LH 750
Workstation Flags
R Review parameter.
Control RETIC Parameters Group Fields

Table 4.50 identifies the field names, test types, and applicable format of Control RETIC
Table 4.50 Control RETIC Parameters -- Group 38

RET% CDR, CR, R X
PN 4277303D 4-59
Table 4.50 Control RETIC Parameters -- Group 38
RET# CDR, CR, R X

MRV CDR, CR, R X
IRF CDR, CR, R X
MI CDR, CR, R *
OTHER CDR, CR, R *
* = Not supported by the LH 750 Workstation format.
Each field in the Control RETICS Parameters Group will have the following format:

Format”
column.
data max. data max.
Result Format: **
Numeric Data:**
Result Comments
RET# >999.9 x 106cells/µL
RET% >100%
Flag Data: **
The flag data characters for the Control RETIC Parameter Group fields will be as follows in
Table 4.51:
4-60 PN 4277303D
Table 4.51 Flag Data for Control RETIC Parameter Group Fields
LH 750
Workstation Flags
R Review parameter.
DIFF Graphics
Table 4.52 identifies the field names, test types, and applicable format of DIFF Graphics
Table 4.52 DIFF DataPlot -- Group 50

DIFF graphics CD, CDR X
If DIFF graphic transmission is disabled, this group will NOT be transmitted.
DIFF Graphics Field:

D I F F H H H • • • H H H Variable .
Retic Graphics
Table 4.53 identifies the field names, test types, and applicable format of Retic Graphics
Table 4.53 Retic DataPlot -- Group 51

Retic graphics CDR, CR, R X
If Retic graphic transmission is disabled, this group will NOT be transmitted.
Retic Graphics Field:

R E T G H H H • • • H H H Variable
PN 4277303D 4-61
HOST PARSING OF TRANSMITTED DATA
4.4 HOST PARSING OF TRANSMITTED DATA

Coulter reserves the right to make additions, deletions, and/or change the order of parameters
in future releases. However, if the communications protocol is adhered to as specified in this
document, future releases will not require reprogramming on the part of the host.
We do not guarantee the appearance of any particular parameter. Neither do we guarantee

any particular order to the transmitted data. This ensures that any program written to parse
an existing transmission format will not have to be restructured if it is designed properly.
Note that each information item is an ASCII character string. Because of delimiters, field
terminators and so on, these information strings may be extracted from the transmitted data
block without having to identify the strings at that time.
4-62 PN 4277303D
5HOST TO LH SERIES WORKSTATION COMMUNICATIONS 5
(HOST ToDo LIST)
5.1 DATA-LINK
Protocol
The LH Series Workstation requires full handshaking to receive data. The protocol is similar
to that used for LH Series Workstation to Host transmissions with the SYN character replaced
with the ENQ character.
The data is transmitted as a sequence of up to 255 blocks of data of 256 [or 128] bytes each.
Generally, these blocks contain 256 data bytes each, but due to the unique needs of differing
hosts, the system is configurable to allow shorter blocks with 128 data bytes. The final block
must be padded with ASCII spaces (20H) if it has less than the number of data bytes
necessary to complete.
Host Computer (SENDER) LH Series Workstation (RECEIVER)

SYN indicates receiver busy, prepare to receive
sample(s) record.
ENQ
ENQ/SYN
(NAK indicates receiver abort}

X X
2-byte data block ACK/NAK
STX 2-byte Exactly 256 or 128 4-byte ETX

blk number data bytes CRC
Send block and await response. Repeat data block and response until done.
ACK/NAK/ENQ
After each block
ENQ
“All Done”
ACK/NAK/ENQ
PN 4277303D 5-1
HOST TO LH SERIES WORKSTATION COMMUNICATIONS (HOST ToDo LIST)
DATA-LINK
DLE
Accept / Reject
The LH Series Workstation ACKs or NAKs each block sent by the Host. If the LH Series
Workstation NAKs a block, the Host must retransmit that block. If the Workstation NAKs the
same block 3 times in a row, the Workstation aborts and is then prepared to receive the next
transmission. If the LH Series Workstation sends an ENQ after the reception of any block, the
Host must retransmit all blocks starting with the first, but should not resend the initial ENQ
character. Some common reasons why the LH Series Workstation would NAK a block are: bad
block sequence number, incorrect block byte count, CRC mismatch, etc. If the Data Link
transmission successfully completes, the LH Series Workstation then transmits a DLE
character followed by a single ASCII character indicating whether the transmission is
accepted or rejected at the presentation level. The ASCII characters following the DLE are
listed below:
The following demonstrates the above protocol:
Host Computer (SENDER) LH Series Workstation (RECEIVER)

ENQ (ready)
ENQ (go ahead)

or
SYN (busy)
# of data blocks
(2 bytes)
ACK (read to receive)

or
NAK (receiver abort)
send data blocks
Data
Block
ACK (block received ok)

or for each block
NAK (retransmit this block)
or
ENQ (retransmit all blocks,
starting with block #1)
5-2 PN 4277303D
DATA-LINK 5
•
•
•
ENQ (all done)
ACK (transmission accepted)

or
NAK (transmission rejected)
(Presentation Level Response)
DLE
‘A’ (transmission accepted, ready for next)
or
‘B’ (transmission accepted, do NOT send more)
or
‘C’ (transmission rejected, retry)
or
‘D’ (transmission rejected, abandon)
or
*‘E’ (transmission rejected, at least one duplicate
pending test request in sample request)
‘F’ (transmission rejected, patient ID already
exists in the LH database)
*Please Note: User may select to replace DLE ‘E’

with DLE ‘C’ in the LIS section of the
Communications Screen in the LH 750
Workstation System Setup. This option is
provided for backward compatibility and is not the
optimum configuration for the interface.
Return Codes
For the Delete request, codes A, B, C, and D still apply. In addition to these, the following
codes are returned for the Delete Function only:
“G” Error in Patient ID field format
“H” Error in Test field format
“I” ToDo list Item not Deleted
PN 4277303D 5-3
PRESENTATION
Data Block Structure
Byte # # of bytes
1 STX 1 byte
2 BLK NBR MS CHAR 1 byte
3 BLK NBR LS CHAR 1 byte
4 DATA BYTES
• 256 (128) C
bytes R
• C
259 (131) •
260 (132) CRC MSB MS CHAR 1 byte
261 (133) CRC MSB LS CHAR 1 byte
262 (134) CRC LSB MS CHAR 1 byte
263 (135) CRC LSB LS CHAR 1 byte
264 (136) ETX 1 byte
5.2 PRESENTATION
Message Structure
PREAMBLE INTRO FIELD 1 0 0 0 FIELD N
The data which is blocked for transmission will consist of a sequence of ASCII lines each
terminated by a CR/LF pair. The block boundaries for the 256 [or 128] byte blocks would
have no special significance with respect to this data. The data may be thought of as a large
ASCII buffer which is being sent 256 [or 128] bytes at a time.
Preamble
Field
SOH
2-byte CR LF
num fields
“00” to “FF”
5-4 PN 4277303D
PRESENTATION 5
Intro
Field
2-byte 2-byte CR LF
record type operation
For ToDo list “AD”

“WL” “UN”
“RU”
“DE”
Each
Field Repeat until all fields given.
ASCII ASCII
Tag Data CR LF
The 2-byte num fields indicate the number of fields that follow the preamble field, that is, the
intro field plus all data fields.
The record type indicates the target data set, for example, "WL" for ToDo list (TDL).
The operation code indicates the action to be performed:
The ASCII Tag is unique to each field within each record type.
DLE Responses From The Workstation And Effects After A ToDo List Download
INTRO FIELD: AD Add or Update
1. Conditions prior to ToDo List Download - No samples are in the ToDo list with the
same Primary Identifier(s) and no samples are in the ToDo List or Completed list with
the same Patient ID.
a. Workstation Response - DLE A or DLE B
b. Effect - Add the downloaded request to the ToDo list.
2. Conditions prior to ToDo List Download - No sample(s) are in the ToDo list with the
same Primary Identifier(s) but there are sample(s) are in the ToDo List and/or
Completed list with the same Patient ID.
b. Effect - Add the downloaded request to the ToDo list. Update the Last Name, First
Name, Gender and Birth Date for all samples in the ToDo List and/or Completed list
that have the same Patient ID.
3. Conditions prior to ToDo List Download - Sample(s) are in the ToDo list with the same
Primary Identifier(s) and sample(s) are in the ToDo List and/or Completed list with the
same Patient ID.
a. Workstation Response - DLE+E, or DLE+C if the workstation was configured to
replace DLE+E with DLE+C
PN 4277303D 5-5
PRESENTATION
b. Effect - Update the Last Name, First Name, Gender and Birth Date for all samples in
the ToDo list and/or Completed List that have the same Patient ID.
4. Conditions prior to ToDo List Download - The downloaded ToDo list format is not
correct.
a. Workstation Response - DLE+C or DLE+D.
b. Effect - No modifications are made to the ToDo list or to the Completed list.
INTRO FIELD: UN Unique
the same Patient ID. If samples are deleted or edited to remove the patient ID then the
Workstation will have to be rebooted before downloading a sample with the same patient
ID using the Unique intro tag. Logging off and back on is not sufficient to allow the reuse
of a patient ID.
same Primary Identifier(s) but there are sample(s) are in the ToDo List and/or
Completed list with the same Patient ID.
a. Workstation Response - DLE F
same Patient ID.
a. Workstation Response - DLE F
4. Conditions prior to ToDo List Download - The downloaded ToDo list format is not
correct.
INTRO FIELD: RU Reuse
the same Patient ID.
same Primary Identifier(s) but there are sample(s) in the ToDo List and/or Completed
list with the same Patient ID.
5-6 PN 4277303D
MESSAGE DEFINITION 5
b. Effect - Add the downloaded request to the ToDo list. Remove the Last Name, First
Name, Gender and Birth Date for all samples in the ToDo List and/or Completed list
same Patient ID.
a. Workstation Response - DLE+E, or DLE+C if the workstation was configured to
replace DLE+E with DLE+C
b. Effect - Remove the Last Name, First Name, Gender and Birth Date for all samples
in the ToDo list and the Completed list that have the same Patient ID.
4. Conditions prior to ToDo List Download -. The downloaded ToDo list format is not
correct.
b. Effect - No modifications are made to the ToDo list or to the Completed list
INTRO FIELD: DE Delete
DE in this field is a command by the host to delete one or more test entries in the LH Series
Workstation ToDo List. See Section 5.4, Delete Message, for a specification for this function.
5.3 MESSAGE DEFINITION

Table 5.1 lists the fields and the format to which they apply. The table also identifies fields
that require data transmission in another field in order to be included in the test request. If
data is not in the associated field, the LH Series Workstation ignores the field. For example, if
a Patient ID is not transmitted, the LH Series Workstation ignores any data included in the
Patient Last Name field.
After the Table is the list of possible fields, their field width and the appropriate ASCII Tag to
be used for transmission.
A field will have a variable length TAG preceding the data separated by one or more SP (20H)
or HT (09H) characters. A field tag must be transmitted with data. If a field tag is transmitted
without data, the Workstation rejects the whole sample request. If an invalid or unrecognized
tag is transmitted, the LH Series Workstation will ignore the field and go to the next field
provided the field to be ignored has the correct field format (i.e. is terminated by a CRLF).
Table 5.1 Message Definition Table
LH 750 Field
Field Name Workstation Requires
Patient ID X Test
Patient Last Name X Patient ID
Patient First Name X Patient ID
Sequence Number X Test
Birth Date X Patient ID
PN 4277303D 5-7
MESSAGE DEFINITION
Table 5.1 Message Definition Table
Sex X Patient ID
Location X Test
Physician Last Name X Test
Physician First Name X Test
User Field 1 X Test
User Field 2 X Test
User Field 3 X Test
Comments X Test
Report Profile X Test
Sample Drawn Date X Test
Sample Drawn Time X Test
Test X
Slide Request X Test
Only one sample’s demographics can be sent per transmission.
FIELDS CANNOT HAVE LEADING OR TRAILING SPACES

(MESSAGE WILL BE REJECTED).
Patient ID Field:

I D X X X • • • X X 16 bytes Patient ID is 16
characters,
alphanumeric.
Value in Intro field
determines what is
done:
AD
UN
RU
See Heading 5.2,
PRESENTATION for
details.
5-8 PN 4277303D
Patient Last Name Field:

P L X X X • • • X X 16 bytes Patient Last Name is 16
characters,
alphanumeric.
Requires Patient ID.
Patient First Name Field:

P F X X X • • • X X 16 bytes Patient First Name is 16
characters,
alphanumeric.
Sequence Number Field:

S N N N N N N N 6 bytes Sequence Number
Birth Date Field:

B D N N / N N / N N N N 10 bytes Date format must be the
same as the
Workstation
configuration. Any of
the following formats
can be used.
DD/MM/YYYY,
MM/DD/YYYY, or
YYYY/MM/DD.
Sex Field:

S X A 1 byte Data Value:
‘M’ - Male
‘F’ - Female
‘U’ - Unknown
‘O’ - Other
(equivalent to
‘Unknown’)
PN 4277303D 5-9
MESSAGE DEFINITION
Location Field:

L N X X X • • • X X 16 bytes
Physician Last Name Field:

D L X X X • • • X X 16 bytes Physician Last Name is
16 characters,
alphanumeric.
Physician First Name Field:

D F X X X • • • X X 16 bytes Physician First Name is
16 characters,
alphanumeric.
User Field 1 Field:

U 1 X X X • • • X X 16 bytes Requires Test.
User Field 2 Field:

User Field 3 Field:

Comments Field:

C M X X X • • • X X 64 bytes Requires Test.
5-10 PN 4277303D
Report Profile Field:

R P N N 2 bytes Report Profile possible
values are from “01" to
"10".
Sample Drawn Date Field:

E D N N / N N / N N 8 bytes Date format must be the
same as the
Workstation
configuration. Any of
the following formats
can be used.
DD/MM/YY,
MM/DD/YY, or
YY/MM/DD.
Requires Test.
If the combined
Date/Time of the sample
is annotated later than
when the actual
Date/Time the sample is
received, the sample
drawn will be rejected.
Sample Drawn Time Field:

E T N N : N N 5 bytes Time output is on 24
hours, regardless of the
system time
configuration. Time
output is
hours:minutes.
Requires Test.
If the combined
Date/Time of the sample
is annotated later than
when the actual
Date/Time the sample is
received, the sample
drawn will be rejected.
PN 4277303D 5-11
MESSAGE DEFINITION
Profile Selection:
Data transmission reflects profile selection. Parameters not in the selected

profile will not be transmitted. Test blocks (e.g., CBC, DIFF, etc.) which are not
in the current profile will not be transmitted. If a complete test block is omitted
from the current profile, the General Information Block (for sample
identification) will still be sent.
Test Field:

T S X X X • • • X X Up to 64 bytes This field indicates test
requests. Format for a test
request is: ”TEST,TUBE
ID,CASS/POS” or
”TEST,TUBE ID,” or
”TEST,CASS/POS” . The
commas are required as
separators. Note that a
comma is required after
TUBE ID, even if it is not
followed by a
CASS/POS.See Table 5.2
for possible values.
Required for
transmission.
The TS tag can repeat
itself for as many tests as
may be required. Only one
test request can be sent
per test tag occurrence.
See Table 5.3 for sample
and text request
combinations.
Slide Request Field:

S R N 1 byte Valid entries are 0 and
1. For a Retic Mode
request, the only valid
entry for the Slide
Request field is 0. If a
value > 1 is sent, this is
converted by the LH 750
Workstation to a value
of 1.
5-12 PN 4277303D
Table 5.2 Possible Values and Examples of Test Requests
Test Tag LH 750 Workstation Comment Example

CBC X CBC only test. “TS CBC,000101”
“TS CBC,123456789012,”
“TS CBC,123456789012,000101”
DIFF X CBC/DIFF test “TS DIFF,000101”
“TS DIFF,123456789012,”
“TS DIFF,123456789012,000101”
CDR X CBC/DIFF/Retic test “TS CDR,000101”
“TS CDR,123456789012,”
“TS CDR,123456789012,000101”
RETIC X Retic only test “TS RETIC,000101”
“TS RETIC,123456789012,”
“TS RETIC,123456789012,000101”
CR X CBC/Retic test “TS CR,000101”
“TS CR,123456789012,”
“TS CR,123456789012,000101”
X = Supported by the LH 750 Workstation format. *CASS POS is six characters TUBEID is no more than 16 characters
Table 5.3 Possible Combinations of Sample Requests with Multiple Test Requests
Test CBC DIFF CDR RETIC CR

CBC NO NO NO YES NO
DIFF NO NO NO YES NO
CDR NO NO NO NO NO
RETIC * YES YES NO NO NO
CR NO NO NO NO NO
* For a Retic Mode request, the only valid entry for the Slide Request field is 0.
PN 4277303D 5-13
DELETE MESSAGE
A sample request with multiple test requests for CBC+DIFF or Retic only, would appear
similar to the following:
.
.
.
“TS DIFF,123456,000101”
“TS RETIC,123,000203”
.
.
.
5.4 DELETE MESSAGE

When the Intro Field of a ToDo List upload from the host contains the Operation string “DE”
(see page 5-77), the host has requested the deletion of one or more entries from the LH Series
Workstation ToDo List. The LH Series Workstation allows deletion of preassigned test orders
from the ToDo List by the host according to;
Patient ID
Sample ID Number
Cassette Position
Test Type
The Sample ID, Cassette Position and Test Type are part of the Test Field. The formats for the
Patient ID and Test fields are described in 5.3 Message Definitions. The Patient ID and Test
field are the only fields of the message definition section used in the Deleted Function. All
others are ignored and need not be sent.
In the Deleted context, an ID or TS Tag followed by a CRLF will be interpreted as an empty

Patient ID or Test Field.
If the Patient ID field and the Test field are empty, the Workstation deletes all entries from
the ToDo List.
According to the above definition, these fields are empty when they have the following form:
Patient ID Field: I D CR LF See Pages 5-5 & 5-9
Test Field: T S CR LF See Pages 5-5 & 5-13
It is possible for a pre assignment to be made to the ToDo List that has no Patient ID. If the
host requests deletion of this pre assignment, or some part of it, the transmission of the
deletion request must contain no Patient ID field. Note that this is not the same as an empty
Patient ID field. No field with an ID tag should be sent. See example 10.
On page 5-83, the Data Format of the Test field is given as “TEST,TUBE ID, CASS/POS” or
“TEST,TUBE ID,” or “TEST,CASS/POS”. The TUBE ID here is the SAMPLE ID. The commas
5-14 PN 4277303D
DELETE MESSAGE 5
are required as separators. Note that a comma is required after TUBE ID even if it is not
followed by a CASS/POS. This Test Field Data Format has additional flexibility in the Delete
Function only.
Note: “TEST,TUBE ID,” represents an empty CASS/POS indicator and “TEST,CASS/POS”

represents and empty TUBE ID indicator.
Additional format flexibility in the Delete Function only is as follows.
“,TUBE,ID,CASS/POS”shall represent an empty TEST indicator.
“,,CASS/POS” shall represent empty TEST and TUBE ID indictors.
“,TUBE ID,” shall represent empty TEST and CASS/POS indicators.
“TEST,,” shall represent empty TUBE ID and CASS/POS indicators.
“,,” shall represent empty TEST,TUBE ID, and CASS/POS indicators.
Note: The last entry has the same effect as an empty Test field.
In the Delete context, an Empty Indicator is interpreted to refer to all values of that indicator
relative to the next highest indicator in the ToDo List. For example; If the TUBE ID indicator
is not empty and the TEST indicator is empty, this is interpreted to mean “delete all TEST’s
preassigned to this TUBE ID”. An empty Test field refers to all preassigned tests relative to a
given Patient ID (this is also true for the Test Field Data Format”,,”).
It should be noted again, that if there is no Patient ID field, this is not the same as an empty
Patient ID field. This is interpreted as referring to a preassignment with no Patient ID.
The following table illustrates the variables.
PN 4277303D 5-15
DELETE MESSAGE
Table 5.4 Patient ID Deletions
Example Patient ID Test ID Action
1 ID_<CR><LF> TS_<CR>< LF> Delete all entries of all types in ToDo list.
2 ID_123456 TS_<CR><LF> Delete all Patient ID “123456” entries of all test types in
<CR><LF> ToDo list.
3 ID_<CR><LF> TS_CBC,,<CR><LF> Delete all CBC tests from all Patient ID entries in ToDo list.
4 ID_123456 TS_CBC,,<CR><LF> Delete all CBC tests from Patient ID 123456.
<CR><LF>
5 ID_123456 TS_CDR,987654,<CR>< LF> Delete CDR test from Tube ID “987654”and all Cass/Pos
<CR><LF> for Patient ID 123456.
6 ID_123456 TS_CDR,,001001,<CR>< LF> Delete all Patient ID “123456” entries of CDR test type in
<CR><LF> ToDo list with Cass/Pos “001001”.
7 ID_123456 TS_CBC,987654,001001, Delete all Patient ID “123456” entries of CBC test type in
<CR><LF> <CR>< LF> ToDo list with Tube ID “987654” and Cass/Pos “001001”.
8 ID_<CR><LF> TS_CBC,001001 Delete CBC test for all the Tube IDs with Cass/Pos
“001001”for all Patient IDs.
9 ID_<CR><LF> * Delete all entries of all types in ToDo list.
10 * TS_ , ,<CR><LF> Delete all entries in ToDo list without Patient IDs.
11 ID_<CR><LF> TS_ ,ID12345,<CR><LF> Delete Tube ID from all test types.
* This field was not transmitted.
Note: TS_<CR><LF> is to TS_, , <CR><LF>
5-16 PN 4277303D
6ASCII Tables 6
6.1 7-BIT ASCII CODES
+ ) ) ) ) ) ) 0 0 0 0 1 1 1 1
* Ú ) ) ) ) ) 0 0 1 1 0 0 1 1
+
* + ) ) ) ) 0 1 0 1 0 1 0 1
Ú
! ! !
7 6 5 4 3 2 1
X X X 0 0 0 0 NUL DLE SP 0 @ P ` p
X X X 0 0 0 1 SOH DC1 ! 1 A Q a q
X X X 0 0 1 0 STX DC2 “ 2 B R b r
X X X 0 0 1 1 ETX DC3 # 3 C S c s
X X X 0 1 0 0 EOT DC4 $ 4 D T d t
X X X 0 1 0 1 ENQ NAK % 5 E U e u
X X X 0 1 1 0 ACK SYN & 6 F V f v
X X X 0 1 1 1 BEL ETB ‘ 7 G W g w
X X X 1 0 0 0 BS CAN ( 8 H X h x
X X X 1 0 0 1 HT EM ) 9 I Y I y
X X X 1 0 1 0 LF SUB * : J Z j z
X X X 1 0 1 1 VT ESC + ; K [ k {
X X X 1 1 0 0 FF FS , < L \ l |
X X X 1 1 0 1 CR GS - = M ] m }
X X X 1 1 1 0 SO RS . > N ^ n `
X X X 1 1 1 1 SI US / ? O _ o DEL
6.2 VALID HOST COMMUNICATIONS ASCII CODES

+ ) ) ) ) ) ) 0 0 0 0 1 1 1 1
* Ú ) ) ) ) ) 0 0 1 1 0 0 1 1
+
* + ) ) ) ) 0 1 0 1 0 1 0 1
Ú
! ! !
7 6 5 4 3 2 1
X X X 0 0 0 0 DLE SP 0 @ P ` p
X X X 0 0 0 1 SOH DC1 ! 1 A Q a q
X X X 0 0 1 0 STX “ 2 B R b r
X X X 0 0 1 1 ETX # 3 C S c s
X X X 0 1 0 0 $ 4 D T d t
X X X 0 1 0 1 ENQ NAK % 5 E U e u
X X X 0 1 1 0 ACK SYN & 6 F V f v
X X X 0 1 1 1 ‘ 7 G W g w
X X X 1 0 0 0 ( 8 H X h x
X X X 1 0 0 1 HT ) 9 I Y I y
X X X 1 0 1 0 LF * : J Z j z
X X X 1 0 1 1 + ; K [ k {
X X X 1 1 0 0 , < L \ l |
X X X 1 1 0 1 CR - = M ] m }
X X X 1 1 1 0 . > N ^ n `
X X X 1 1 1 1 / ? O _ o DEL
Bold used only for Data-link control.
PN 4277303D 6-1
ASCII Tables
VALID HOST COMMUNICATIONS ASCII CODES
6-2 PN 4277303D
7CRC 7
7.1 CRC ALGORITHM
The CRC algorithm used is a modified CCITT CRC16 algorithm and is the same as used in
the STKS™ analyzer.
The polynomial for this algorithm is: X**16 + X**12 + X**5 + 1.
Note: D = current data byte that is input to the algorithm.
CRCLSB,CRCMSB = data byte. Least significant and most significant CRC accumulator
bytes.
x>>n means x is shifted n bits to right or is the same as x divided by 2n.
x<<n means x is shifted n bits to left or is the same as x multiplied by 2n.
//------------------------------------------------------------------------------------------------------------------
at beginning,
CRCLSB = 0FFH (octal 377) (decimal 255)
CRCMSB = 0FFH (octal 377) (decimal 255)
then for each data byte in a block,

X = D XOR CRCMSB
X = X XOR ( X >> 4 )
CRCMSB = CRCLSB XOR ( X >> 3 ) XOR ( X << 4 )
CRCLSB = X XOR ( X << 5 )
and at end,
CRCLSB = CRCLSB XOR 0FFH
CRCMSB = CRCMSB XOR 0FFH
//------------------------------------------------------------------------------------------------------------------
PN 4277303D 7-1
CRC
CRC EXAMPLE WRITTEN IN ASM86
7.2 CRC EXAMPLE WRITTEN IN ASM86

The following is an example of CRC16 code written in assembler for 8086:
;-------------------------------------------------------------------------------------------------------------------
GET_CRC_BYTES PROC
PUSH CX
;;This is the Algorithm used (CCITT CRC16):

;
; X = D XOR CRCMSB
; X = X XOR ( X >> 4 )
; CRCMSB = CRCLSB XOR ( X >> 3 ) XOR ( X << 4 )
; CRCLSB = X XOR ( X << 5 )
;
;Data arrives in AL.
;Finished CRC is in BX ( BH = MSB, BL = LSB )
;To use this algorithm:
; 1. Initialize BX to 0FFFFH.
; 2. At end:
; CRCLSB = CRCLSB XOR 0FFH.
; CRCMSB = CRCMSB XOR OFFH.
;
XOR AL,BH ;X=D XOR CRCMSB (AL=X) <--- 1
MOV AH,AL ;Save X for later in AH

SHR AL,4 ;Then AL=X >> 4
XOR AL,AH ;X=X XOR ( X >> 4 )<--- 2
MOV AH,AL ;Save X

MOV CH,AL ;Save X
SHR AL,3 ;Then AL= ( X >> 3 )
XOR AL,BL ;AL=CRCLSB XOR ( X >> 3 )
SHL AH,4 ;AH=( X << 4 )
XOR AL,AH ;CRCMSB=CRCLSB XOR

MOV BH,AL ; ( X >> 3 ) XOR ( X << 4 )<--- 3
MOV AL,CH ;Recover X in AL

SHL AL,5 ;AL=( X << 5 )
XOR AL,CH ;AL=X XOR ( X << 5 )
MOV BL,AL ;CRCLSB=X XOR ( X << 5 )<--- 4
POP CX
RET
GET_CRC_BYTES ENDP
;-------------------------------------------------------------------------------------------------------------------
7-2 PN 4277303D
CRC
CRC EXAMPLE WRITTEN IN C 7
7.3 CRC EXAMPLE WRITTEN IN C
The following is an example of CRC16 code written in C.
//------------------------------------------------------------------------------------------------------------------
void calc_crc ( unsigned char DataByte )
{
DataByte ^= crcmsb;
DataByte ^= ( DataByte / 16 );
crcmsb = crclsb ^ ( DataByte / 8 ) ^ ( DataByte * 16 );
crclsb = DataByte ^ ( DataByte * 32 );
}
//------------------------------------------------------------------------------------------------------------------
PN 4277303D 7-3
CRC
CRC EXAMPLE WRITTEN IN C
7-4 PN 4277303D
8GRAPHIC RECONSTRUCTION 8
8.1 RBC, WBC THRESHOLD, AND PLT HISTOGRAMS
The histograms display a simple line graph which is representative of the count of events
occurring in each A/D channel (or voltage level). The histograms are transmitted as 512
ASCII bytes for RBC (256 bytes for PLT, 112 bytes for WBC and 512 bytes for WBC Full
Histogram) representing a 256-byte for RBC (128-byte for PLT, 56 bytes for WBC and 256
bytes for WBC Full Histogram) Hex array. It takes two ASCII bytes to represent one Hex byte.
Each Hex byte represents a scaled count for each channel 0-255 (14-142 for PLT). The
histograms can be constructed by plotting the count for each channel then drawing a line
between plots.
To display (or print) the histogram you must plot the channel vs. count. The channel
represents the X-axis while the count is the Y-axis (see Figure 8.1). There are 128 channels for
the platelet histogram, 256 channels for RBC, 56 channels for WBC and 256 channels for
WBC Full Histogram. The value range is 14-255 (0D-FF hex) for the platelet histogram and
0-255 (00-FF hex) for the WBC and RBC histograms.
Figure 8.1 Plotting RBC, WBC Threshold and PLT Histograms
y
C
O
U
N
T
<< x
CHANNEL
8.2 LH 750 DATAPLOT FORMAT

The 2-dimensional dataplots communicate a greater amount of information to the viewer
about the events. The following sections describe the format of the dataplot data and provide
a how-to example of displaying the dataplot data on a raster device such as a video display or
printer.
Dataplots
The dataplots consist of two major components: dataplot data and rendering information.
Taken together, these two components allow a receiving application to display the dataplots
in the same fashion as the LH Series Workstation.
The dataplot portion of the host transmission consists of a dataplot data header and one
dataplot data block.
PN 4277303D 8-1
GRAPHIC RECONSTRUCTION
LH 750 DATAPLOT FORMAT
Dataplot Data Header

The dataplot data header tells the receiving application how large the dataplot transmission is
and how many dataplot data blocks are contained in the transmission. A complete dataplot
transmission, including the dataplot data header, is illustrated in Figure 8.2.
Figure 8.2 Dataplot Transmission
Dataplot data header

Dataplot data block
Dataplot Data Header Elements

The dataplot data header contains two elements: the total length of the dataplot transmission
and the number of dataplot data blocks in the transmission. The data are represented as one
16-bit integer which indicates the total size of the dataplot transmission and one 8-bit integer
which indicates the total number of dataplot data blocks in the transmission (between 1 and
4). Only one block is supported currently, but up to four may be used in future software. See
Figure 8.3 for details.
Figure 8.3 Dataplot Data Header
Transmission Length LSB

Transmission Length MSB
Total Number of Dataplot
data blocks
Dataplot Data Block

The dataplot data block contains both the rendering information and the actual
2-dimensional dataplot data. Each dataplot data block is composed of four subsections:
dataplot data block header, rendering information block, dataplot header and the dataplot
data. Figure 8.4 illustrates the dataplot data block format.
Figure 8.4 Dataplot Data Block Format
Dataplot data block header

Rendering information
block
Dataplot header
Dataplot data
8-2 PN 4277303D
LH 750 DATAPLOT FORMAT 8
Dataplot Data Block Header
The dataplot data block header tells the receiving application about the type of dataplot being
transmitted as well as details about any included rendering information. See Figure 8.5. The
dataplot data block header has the following format:
Figure 8.5 Dataplot Data Block Header
Dataplot Type code Dataplot Type Code is an 8-bit integer which represents the
run type of the sample which produced this dataplot. It will
be one of the following values:
SC_TYPE_5DIFF (0x01) is 5 Part Differential Dataplot.

SC_TYPE_RETIC (0x02) is Reticulocyte Dataplot.
SC_TYPE_CD4 (0x04) is CD4‡ Subset Dataplot.
SC_TYPE_CD8 (0x05) is CD8‡ Subset Dataplot.
Dataplot Option code Dataplot Option Code is an 8-bit integer which contains
option flags that pertain to the current dataplot. The
following flags are currently defined and can be combined
into Dataplot Option Code with a logical or when applicable:
SO_COMPRESSED (0x01) indicates that the dataplot

has been compressed. This option is not supported
currently, but may be available in future software.‡
SO_HAS_RENDER_INFO (0x02) indicates that a valid
rendering information block follows.
Dataplot width (LSB) Dataplot width is a 16-bit integer which specifies the width
of the dataplot.
Dataplot width (MSB)
Dataplot height (LSB) Dataplot height is a 16-bit integer which specifies the height
of the dataplot.
Dataplot height (MSB)
‡This symbol indicates that at the time of this release this feature was not available.
Rendering Information Block

The rendering information block contains information the receiver can use to reconstruct the
dataplot as it was displayed by the LH Series Workstation. The dataplots are color-coded to
identify the population and dithered to code the density. The rendering information block
contains color palettes and dither pattern bitmaps used by the LH Series Workstation
software when displaying or printing the dataplots. It is highly recommended that host
software employ the same scheme when displaying the dataplots to maintain consistency of
operator conclusions across platforms. See Figure 8.6. The structure of the rendering
information block is as follows:
PN 4277303D 8-3
Figure 8.6 Rendering Information Block
Rendering information
block header
Color palette
Dithered display bitmap
library
Rendering Information Block Header

The rendering information block header contains fields which indicate the size and format of
the bitmaps and the color palette. It is sent on the first transmission after a user log-on and a
shutdown/restart occurs. As notification is not provided when a shutdown/restart is initiated,
the rendering block transmission cannot be predicted in advance. Figure 8.7 is an illustration
of the rendering information block header.
Figure 8.7 Rendering Information Block Header Format
Rendering information Rendering Information Block Options may be one or more of

block options the following flags:
RH_OPTIONS_NONE (0x00) indicates that no options

are set.
It is an 8-bit integer.
Number of color palette Number of Color Palette Table Entries is the number of
table entries entries in the color palette. It is an 8-bit integer.
Number of bitmap library Number of Bitmap Library Entries is the number of bitmap
entries patterns contained in the Dithered display bitmap library. It
is an 8-bit integer.
Bitmap width in pixels Bitmap Width in Pixels is the width of each bitmap in pixels.
Bitmap height in pixels Bitmap Height in Pixels is the height of each bitmap in
pixels. It is an 8-bit integer.
Color Palette
Each color palette entry contains three 8-bit integers which represent the red, green and blue
values for that index as shown in Figure 8.8.
Figure 8.8 Palette Table Entry
Red Component
Green Component
Blue Component
Dithered Display Bitmap Library

The dithered display bitmap library contains the dithering bitmaps used by the LH Series
Workstation to display the dataplots. They indicate the density of a specific cell population.
Receiving applications should use these bitmaps when generating displays to ensure that host
8-4 PN 4277303D
LH 750 DATAPLOT FORMAT 8
displays will match LH Series Workstation displays even when Coulter updates the dither
patterns or population colors. The bitmap format is a standard raster scanned array of pixels.
Each value is an 8-bit integer which corresponds to an entry in the color palette described
above. Figure 8.9 describes the bitmap format.
Figure 8.9 Bitmap Format
Bitmap width
0 in pixels
Bitmap height
in pixels 7306002B
Each bitmap is stored as a column-major array of pixels represented as 8-bit integers. The
array contains:
Bitmap width in pixels x Bitmap height in pixels 8-bit integers
Dataplot Header
The dataplot header contains instructions of how the dataplot data is to be interpreted. The
structure of the dataplot header is shown in Figure 8.10.
Figure 8.10 Dataplot Header
Dataplot data options Dataplot data options may be one of the following flags:
SO_OPTIONS_NONE (0x00) no option have been

selected.
Dataplot datum size Dataplot datum size is the size in bits of each point in the
dataplot. It is usually 8 which represents a byte. It is an 8-bit
integer.
Reserved Reserved for future use and should be ignored. It is an 8-bit
integer.
Reserved Reserved for future use and should be ignored. It is an 8-bit
integer.
PN 4277303D 8-5
The format of the dataplot is shown in Figure 8.11.
Figure 8.11 Dataplot Data Format
Scatterplot
0
width
Scatterplot
height 7306003B
8-6 PN 4277303D
TRADEMARKS
5C Series Control, Beckman Coulter, Coulter, Coulter Clenz, Coulter Counter, Lin-C, Lyse S,
S-Cal, Z Series, Zap-Oglobin are trademarks of Beckman Coulter, Inc.
PN 4277303D
COULTER LH SERIES SYSTEM HARD-COPY DOCUMENTATION
s Getting Started Overview of system hardware and software.

PN 4277302 (LH 750)
PN 624601 (LH 500)
s Host Transmission Specifications for transmitting to a host computer
PN 4277303
s Reference Use and Function • Installation • Operation Principles • Specifications/Characteristics •
PN 4277248 (LH 750) Precautions/Hazards • References • Glossary • Index
PN 624604 (LH 500) Available in hard copy by request.
s Operator’s Guide/IFU Controls and Indicators • Startup • QC • Sample Analysis • Data Analysis • Shutdown •
PN 4277249 (LH 750) Analyzer CRT Functions • Workstation • Run Samples Display • To Do List • Database •
PN 624602 (LH 500) Controls • Setup • Appendices
Available in hard copy by request.
s Special Procedures and Calibration • Cleaning Procedures • Replace/Adjust Procedures • Troubleshooting •
Troubleshooting Error Messages
PN 624603 (LH 500)
s SlideMaker Operator’s Use And Function • Installation • Operation Principles • Specifications/characteristics •
Guide Operating Procedures • Cleaning/Replacement Procedures • References
s SlideStainer Operator’s Use And Function • Installation • Operation Principles • Specifications/characteristics •
Guide Operating Procedures • Cleaning/Replacement Procedures • References
s Master Index Combined index for Reference, Operator’s Guide, and Special Procedures and
PN 4277301 (LH 750) Troubleshooting manuals. Comes with hard copy of Operator’s Guide and Special
PN 624605 (LH 500) Procedures and Troubleshooting.
r Operator Manuals HTML file of the System Help • PDF files of the Reference manual, Instructions for
CD-ROM Use, and Special Procedures manual.
PN 6418430 (LH 750)
PN 624495 (LH 500)
The Getting Started booklet and the Host Transmission manual come with your LH Series System.
The information in the Reference manual, Operator’s Guide, Special Procedures and Troubleshooting manual, SlideMaker
Operator’s Guide and the SlideStainer Operator’s Guide comes from the Online Help System.
Printed on Recycled Paper

LH Series Host Transmission Specification

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COULTER® LH Series WorkStation

PN 4277303D (September 2004)

HAZARDS AND OPERATIONAL PRECAUTIONS AND LIMITATIONS

WARNING Risk of operator injury if:

Initial Issue, 7/01

REVISION STATUS, iii

1.1 PURPOSE, 1-1

2 HARDWARE INTERFACE, 2-1

2.1 HARDWARE HANDSHAKE, 2-1

3 COMMUNICATION PARAMETERS, 3-1

3.1 COMMUNICATION PARAMETERS, 3-1

3.2 COMMUNICATION PROTOCOLS, 3-1

3.3 TIME-OUT, 3-2

3.4 BAUD RATE, 3-2

3.5 DATA BITS, 3-2

3.6 PARITY, 3-2

3.7 STOP BITS, 3-2

3.8 HANDSHAKE, 3-2

3.9 BLOCK SIZE, 3-2

3.10 GRAPHICS, 3-2

3.11 COMPATIBILITY, 3-3

3.12 SPOOLER, 3-3

3.13 CLEARING THE SPOOLER, 3-3

3.14 REPLACE NULL BY SP, 3-3

3.15 DISABLING HOST COMMUNICATIONS, 3-3

3.16 ENABLE VALIDATION CODES, 3-3

3.17 REPLACE DLE E WITH DLE C, 3-4

3.18 COLLATE THE TODO LIST, 3-4

3.19 REPLACE V AND V FLAGS WITH R (LH 500 ONLY), 3-4

4 LH SERIES WORKSTATION TO HOST COMMUNICATIONS, 4-1

4.1 DATA-LINK, 4-1

Full Software Handshake, 4-1

4.2 1G.1 + RETICS FORMAT, 4-4

4.3 LH 750 WORKSTATION FORMAT, 4-25

Control CBC Parameters Group Fields, 4-56

4.4 HOST PARSING OF TRANSMITTED DATA, 4-62

5 HOST TO LH SERIES WORKSTATION COMMUNICATIONS

5.1 DATA-LINK, 5-1

5.2 PRESENTATION, 5-4

5.3 MESSAGE DEFINITION, 5-7

5.4 DELETE MESSAGE, 5-14

6 ASCII Tables, 6-1

6.1 7-BIT ASCII CODES, 6-1

6.2 VALID HOST COMMUNICATIONS ASCII CODES, 6-1

7.1 CRC ALGORITHM, 7-1

7.2 CRC EXAMPLE WRITTEN IN ASM86, 7-2

7.3 CRC EXAMPLE WRITTEN IN C, 7-3

8 GRAPHIC RECONSTRUCTION, 8-1

8.1 RBC, WBC THRESHOLD, AND PLT HISTOGRAMS, 8-1

8.2 LH 750 DATAPLOT FORMAT, 8-1

Dataplot Header, 8-5

COULTER LH SERIES SYSTEM HARD-COPY DOCUMENTATION, 2

r STKS 1G.1 + Retics

The LH SeriesWorkstation/Host communications uses only the following signals:

P3 Pin No. Signal Name Flow Direction

2.1 HARDWARE HANDSHAKE

Diagram of Hardware Handshake Mode

LH Series Workstation (SENDER) Host (RECEIVER)

3.2 COMMUNICATION PROTOCOLS

Low Level Protocol

High Level Protocol

DO NOT ASSUME FIXED OFFSETS

Table 3.1 lists the communication parameters available by format.

Communication Parameter 1G.1 + Retics LH 750 Workstation