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HNF 152
FOOD AND NUTRITION RESEARCH
DECEMBER 2018
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TABLE OF CONTENTS
PAGE
TITLE PAGE i
TABLE OF CONTENTS ii
LIST OF FIGURES iv
LIST OF APPENDICES v
ABSTRACT vi
CHAPTER 1. INTRODUCTION 1
Problem Statement 2
Hypothesis 5
Ginger 6
Introduction 6
Rhizome Characteristics 6
Pectin 9
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PAGE
Applications 10
Marmalade 10
Hedonic Scale 12
Research Design 13
Sampling 14
Target Population 14
Sampling Method 14
Sample Size 14
Panelists 15
Data Collection 15
Sensory Evaluation 15
Measurement 16
Pre-testing 16
Data Analysis 16
Recommendations 22
LITERATURE CITED 24
APPENDICES 26
LIST OF TABLES
TABLE PAGE
iii
LIST OF FIGURE
FIGURE PAGE
1 Structure of Oleoresin 7
2 Structure of Pectin 9
iv
LIST OF APPENDICES
APPENDIX PAGE
A Timeline of Research 26
v
ABSTRACT
vi
CHAPTER 1
INTRODUCTION
primarily in foods and beverages due to its distinct flavor and pungent aroma. It is
native to tropical countries in Asia including China and the Philippines. Ginger extracts
contain gingerols and phenolic compounds, which are responsible for its flavor and
aroma (Sukumaran, Park, & Giri, 2016). The plant is known for its anti-inflammatory,
gustatory and anti-diabetic property. The pharmacological effects of its compounds and
mechanistic aspects of health have been verified and studied experimentally in recent
years (Srinivasan, 2017). Two wide categories can be derived from fresh ginger roots,
2013).
The pungent aroma and distinct flavor of ginger make it indispensable in the
powder, and ale which is an ingredient of carbonate drinks, alcoholic drinks and
distinction of the use of chunks or bits. Citrus fruits rich in pectin are the primary
jackfruit could also be used (Black, 2015). Pectin is a structural polysaccharide found
on cell walls of terrestrial plants. The compound is known for it’s thickening and gelling
manufacturing industry for its gelling capacity. Known usage of pectin includes
marmalades, jams, preserves, low calorie jellies, and confections (Besson, Yapo, &
Kofi, 2013).
aluminium lids. Common fruit ingredients include oranges, strawberry, pineapple, and
the market. With the rise of in the competition among food industries, professionals
and manufacturers are faced with the challenges of producing foods containing
health challenges and satisfy the market acceptability and palatability of products
(Chinma & Gernah, 2007 as cited by Oyetoro, 2016). Both the consumers and food
There is now ample research on the benefits of ginger and the marketability of
its derived products. It has been found out that Zingiber possesses mechanical
and anti-tumor properties, which is directly linked to beneficial effects in the heart,
cancer treatment, diabetes management, and bacterial infection eradication (Bode &
Dong, 2011). However, limited research has been reported on the general
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Ginger-based products has been developed for market availability and
ginger gives rise to the various food innovations in the processing, production and,
consumption. Acceptability and palatability of these sweets are not widely focused on
studies and sensory evaluation on consumer preferences will aid in the lagging
1.1 age
1.2 gender
sample
The primary objective of this research was to asses the level of acceptability
and palatability of each ginger marmalade sample. The specific objectives are:
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4. to determine the level of acceptability and palatability of ginger marmalade
Consumers. The results of this study will enable the consumers to know the
production will be aware on the consumer preference that will enable them to
Food Technologists and Nutritionists. The outcome of the study will aid
these groups to formulate and innovate ginger food products based on its
Since the evaluators of the product are not experts, the sensory evaluation
was added to the ginger marmalade hence maintaining its natural flavor and aroma.
The nutritional content and moisture of the raw material and the product was tested.
Since the study focused on a sugar-rich product, the effects of too much intake of
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marmalade will be noted to avoid potential health effects including dental carries,
1.6. HYPOTHESIS
The hypothesis are stated below. The null hypothesis (Ho) indicates that there is
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CHAPTER 2
LITERATURE REVIEW
2.1. GINGER
2.1.1. Introduction
Ginger (Zingiber officinale), is a flowering plant whose rhizomes are used primarily in
foods and beverages due to its distinct flavor and pungent aroma. It is native to tropical
countries in Asia including China and the Philippines. Ginger extracts contain gingerols and
phenolic compounds. The plant is known for its anti-inflammatory and anti-diabetic property
Two wide categories can be derived from fresh ginger roots, namely; volatiles and non-
hydrocarbons give ginger pungent aroma and distinct taste (Mashhadi et al. 2013).
capable of reproducing shoots and roots systems different from the parent plant. This is the
plant’s storage form of starch and protein, which is used as its nutrition source (Hemminga,
1997).
Ginger rhizome exhibits the plant’s pungent aroma and flavor. These characteristics
are attributed to the presence of essential oils and oleoresin. The chemical composition of
oleoresin is chiely shogaols and gingerols (Balladin & Headley, 1997 as cited by Azian,
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Oleoresin, a light-yellow to brown liquid, is one of the major oil extracted from ginger
rhizome. It is a non-polar compound soluble in oils and other non-polar solvents and has
phosphorous, fluorine and chlorine are present in rhizomes. Vitamin C predominates other
Temperatures that are relatively low facilitate the greater preservability of rhizome’s
chemical components. Prior to the exposure to low temperature conditions, the solar energy
from the sun in the form of heat dehydrates the moisture content of rhizomes to 10% thus
concentrating the amount of oleoresin. Dehydration of the plant part via the use of a low
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2.1.3 Market Forms of Ginger
Ginger is used in numerous products due to its versatility. Primary products include
fresh ginger and dried hinger. Asians consume most of the fresh ginger produced. This is
used as herb in traditional dishes in east and southeast part of the continent. Dried ginger,
which is usually in the powdered form, is commonly used and consumed in India, China,
Australia, Africa, and the Caribbean. This type is used as spice for its relatively high
Secondary and derived products of ginger are candies and preserves, essential oil and
oleoresin, ginger paste, and nutraceuticals. Syrups from ginger are used in China, and
candied rhizomes, which are regarded with superior and consistent quality, are developed
and exported by Australia. Essential oils, which are obtained by the process of distillation, is
used as spice and ingredients for soft drinks and beer. Through solvent extraction, oleoresin
use ginger as component of medicines for nausea, motion sickness, and migraine ((Food and
Agriculture Organization of the United Nations, 2002). Based on the discussions above the
2.2 PECTIN
walls of terrestrial plants. The compound is known for it's thickening and gelling ability.
Pectin is generally a water soluble fiber, which aids in heart and diabete sprotection,
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weight loss, and healthy bowel movement (Maxwell, Belshaw, Waldron, & Morris,
2012)
Fruits and vegetables are rich in pectin, examples of which are beets, apple,
cucumber, onion, pea, tomatoes, pears, leeks, and citrus fruits like orange and lemon.
Unripe fruits contain protopectin which gives its tough texure and limited gelling
capacity. Upon ripening of the fruits, the enzyme pectase acts on protopectin and
yields pectin which gives the ripe fruits its soft texture and gelling ability. (Claudio, Ruiz,
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2.2.2. Applications
used primarily in making marmalades, jams, preserves, low calorie jellies, and
Films made from the combination of starch and pectin are recyclable and
biodegradable. They are known for their tensile and good modulus properties. Starch is
incorporated with hot water to facilitate gelatinization before being mixed with pectin.
crystalline molecules thus favoring polymerization (Coffin et al., 1995 as cited by Kaur,
2011). These films are used as ingredient in noodles and edible soup bags.
2.3. MARMALADE
pieces, or crushed and adding foodstuff such as sweetening agents and pectin sources
must constitute at least 30% of the total volume of the ingredients or the final product
except for ginger that is at 11% of the total volume. When fruits are mixed together, the
final product should at least constitute 35% of the total product with the exception of
ginger (11%), citrus fruits (6%), cranberry and soursop (20%), and durian (10%). The
minimum content must be used and reduced in proportions to percentages that is used
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On a study on the sensory property of ginger preserves and candies, it has
been found out that the higher concentration of sugar in the preserves gives better
quality preserve based on the color, flavor, texture, and overall acceptability. The
sample constitutes 60%, 65%, and 70% of sugar in each sample (Siddiqui, Bhuiyan,
One way of preserving and canning foods is by boiling water bath canning. This
submerged in water contained in a boiling water bath canner. The canner is allowed to
boil at 100 °C (5 to 85 minutes). The time of boiling depends on the size of the jar and
the type of food to be preserved. Removing the oxygen from the jar and killing
vegetative bacteria is the primary role of boiling canned products (Boyer & McKinney,
2013).
Water Bath Canning method as seen in Figure 2.4. Highly acidic items are those
whose pH is less than or equal to 4.6. Foods with pH higher than 4.6 is a medium of
growth of potent harmful spores from bacteria which produces toxins. Boiling thus not
kill bacteria, Clostridium botulinum, commonly present in canned products (Boyer &
McKinney, 2013).
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Figure 3. Boiling Water Bath Canning (Clemson University, 2013)
9-Point Hedonic Scale, also known as Hedonic scale is a test and rating scale
with nine points, which bear a given word descriptions. This ranges from “very much
dislike” to “very much like.” The evaluators’ opinion about the food qualities are
emphasized than the food item itself. The method is commonly done in consumer
preference tests for new products to be introduced in the market (Chambers, 1998).
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CHAPTER 3
MATERIALS AND METHODS
The study was conducted at the Institute of Human Food and Nutrition (IHNF),
University of the Philippines Los Banos (UPLB) under the supervision of Dr. Marites Yee. The
sensory evaluation was done at the Human Care Laboratory, IHNF, UPLB
Ginger (Zingiber officinale) rhizome was sourced from the local market of Los Banos,
Laguna, and was utilized in making ginger marmalade. Fruit pectin in the form of lemon was
purchased from the same market. Commercial sugar (Victoria) was obtained from Victoria
Milling.
The preparation of the sample was done on October 19, 2018 and the sensory
Three proportions of ginger 1/2 cup (24%), 1/4 cup (13%), and 1/8 cup (7%) of equal
parts of grated and diced ginger was measured using a standard measuring cups. The last
proportion of ginger is below the recommendations set by the Food and Agriculture
Department (FAO) on codex alimentarius. Each proportion was placed in a separate sauce
pot with 1 1/2 cup of sugar and 1/8 cup of lemon extract (6-7% of the volume depending on
the sample). Each mixture was allowed to boil and to caramelize at 410 F for 35 minutes until
honey-thick.
The cooked marmalade was cooled to room temperature and was placed in separate
glass jars which are sterilized in a steamer for 30 mins at 100 C. The samples were subjected
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The product was tested for palatability and acceptability through purposive sampling of
respondents which undergone sensory evaluation. The results was statistically treated to
3.2. SAMPLING
The participants was sourced from individuals that are present in near the sensory
evaluation are. All of the available purposely selected participants were considered to be
consumer panelists, who happen to be in the testing site and are willing to participate in the
study.
A combination of purposive sampling and availability sampling was used to obtain the
constituents. The other method is also known as haphazard or accidental. Inside the scope of
UPLB constituents, those who are readily available to the testing site was considered
respondents when they agreed to evaluate the product. One place, HCL, was used to
The sample size was obtained using a sample size recommendation Gengler, 2010 in
her study When People are Instrument: Sensory Evaluation Methods. According to the study,
the sample size depends on the question problem. Sensory evaluations that aims to
determine how a product developed is to be likely, a sample size of 30-100+ respondents who
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are represent end users. The minimum required respondents of thirty (30) was used in the
study.
3.2.4. Panelists
The population of 30 panelists are between the ages of 19 and 26. Five respondents
were at the age of 19, eleven were 20, three are 22, and one for both 23 and 26 years old.
Thirty percent (30%) or nine of the respondents are male and seventy percent (70%) or 21
respondents are female. This resulted that the age of the respondents is about 21 yrs old, on
The participants completed the consent form and sensory evaluation forms individually
through sensory tests that involves the appearance, texture, taste, and overall acceptability.
The form also contains basic socio-demographic questions such as age, sex, and food
preferences.
Nine-Point Hedonic scale testing was used by the panelists in testing the three ginger
marmalade food samples. The panelists were asked to taste each sample spread on a soda
cracker, and record the results in the sheet of questionnaire provided. After each sample
evaluation, water was given to each of the respondents. The product was evaluated with its
The panelists were asked to rate the different criteria presented on a 9-point hedonic
scale with the ratings of: like extremely, like very much, like moderately, like slightly, slightly,
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neither like nor dislike, dislike slightly, dislike moderately, dislike very much, and dislike
extremely.
During the evaluation period, the consumers were asked to differentiate the three given
samples that is coded A,B, and C with proportions of 1/2, 1/4, and 1/8 cup respectively, and
indicate the most and least preferred. After the food profiling and comparison, the panelists
were requested with their age, sex, and food preference, in order profile the participants’
insights.
3.3.2. Measurement
measurement device. The questionnaire is composed of three separate hedonic scales and
questions relevant in the study that can be used for analysis of data from the respondents.
3.3.3. Pre-testing
With the defined sample size of 30 respondents, pre-testing was done to 20% of the
calculated sample size which is 6 respondents. The pre-testing was done on the IHNF Lobby,
IHNF, UPLB. Each will be given the same set of questionnaires and 9-Point Hedonic Scale
evaluation sheet.
The profiling of participants was done to determine the frequency of each socio-
demographic characteristics of the panelists. Hedonic scale data was analyzed using the
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differences between two or more groups with independent variables. The sensory
to determine what sample caused the significance. Sex and food preferences was subjected
to Cramer’s V with age as ETA coefficient. This test is used to measure association between
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CHAPTER 4
RESULTS AND DISCUSSION
The appearance, texture, taste, and overall acceptability of three ginger marmalade
samples were evaluated by the thirty (30) panelists. Each respondent was briefed and was
given a random coded sample of A, B, and C. Sample A was made from 23% ginger, sample
B was made from 13%, and sample C was made from 7% ginger, which is below the codex
standards. The Kruskal-Wallis test was performed for appearance, texture, taste, and overall
acceptability.
Table 2. Kruskal-Wallis test of significance for appearance, texture, taste, and overall
acceptability.
evidence to say that there is no difference in the median scores of three ginger marmalade
samples for both the appearance and texture sensory characteristics. Both the characteristics
have a P value of <0.05. On the other hand, Taste and overall acceptability showed statistical
significance. The P value (0.0152 < 0.05) for taste and P value (0.0106 < 0.05) for overall
acceptability have difference in the median scores among the three samples presented.
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The differences on the median score of both taste and overall acceptability in the three
samples can be attributed to the different amounts of ginger in each sample. To know what
causes the significance, Mann Whitney U-test on P values was performed for all the samples.
Table 3. Mann Whitney U-test of p values for taste and overall acceptability of samples.
Overall
0.642 0.015 0.007
Acceptability
Note: p values are at 5% level of significance
Based on the table above, the difference on median scores based on the P values
calculated can be attributed on sample C due to the fact that there was no statistical
significance between sample A and B, and there was a statistical significance when Sample C
The amount of ginger in Sample A, B, and C are 24%, 13%, and 7% respectively. Both
sample A and B are within the recommended amount of ginger which is set at a minimum of
11% of the total volume of the raw material or the product. On the other hand, sample C is
below the set minimum amount standard by the Food and Agriculture Organisation (FAO)
based on Codex Alimentarius. This characteristic may be the reason behind the least
preference for sample C and difference on the median scores for both taste and overall
acceptability.
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4.2. Effect of age, sex, and food preferences to sample preferences
Table 4 shows that the sample/s most preferred by the respondents are samples A and
B while Sample C was the least preferred among the panelists. In order to determine the
effects of age, sex, and food preferences to sample preferences of the respondents, Cramer’s
Based on table 5 age has a coefficient of 0.325 because 32.5% in the variation of the
sample preference is explained by the age factor. While the remaining 67.5% is accounted for
the variables not included in the study. There is a moderate association on age with sex and
like sweet and spicy and a strong association on like ginger. The results showed no statistical
significance on the age based on food preferences to sex and food preferences. So these
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CHAPTER 5
SUMMARY AND CONCLUSION
The acceptability and palatability of ginger marmalade was determined using the 9-
point hedonic scale, ranking the samples based on appearance, taste, texture, and overall
acceptability. Ginger concentration showed a significant effect the overall acceptability and
texture of ginger marmalade samples. The least amount of ginger on sample C, which is
below the set standards, is determined as the least acceptable and palatable among the
samples. Both samples which are above the recommendations showed an acceptability and
Significant differences among sample’s taste, and overall acceptability can be seen.
This significance is brought by the sample which the respondents like the least. Within the
sample size’s socio-demographic data, sex and food preferences have no effect on the
5.1. Recommendations
cannot be made for a population but is only limited to the sample size. A probability type of
sampling would be more appropriate for the study to eliminate possible sources of bias and
errors.
proximate test for vitamin C and moisture content to determine its protein, carbohydrate, and
fat content. To be able to compare it to published data on ginger moisture content and vitamin
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The storage stability of the samples can also be measured by storing it up to 120 days
and constantly noting the color, flavour, and fungal growth every 15 days on the first 60 days
and 30 days on the succeeding periods (Siddiqui, Bhuiyan, and Easdani, 2012).
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LITERATURE CITED
JOURNAL SOURCES
Ajala, A.S. & Ajao, I.A., (2012). Production and Quality Evaluation of Ginger-Flavored
Banana Marmalade. International Journal of Emerging trends in Engineering
and Development, 7 (2012), 579-584
Azian, M. N., Kamal, A. A. M., & Azlina, M. N. (2004). Changes of cell structure in ginger
during processing, 62, 359–364. doi:10.1016/S0260-8774(03)00251-6
Besson V., Yapo B. M., Koffi K. L. Extraction of Novel Galactoarabinan-Rich Pectin with
Gelling Capacity from Soursop Pomace. Natural Products Chemistry &
Research 1:117 doi:10.4172/ 2329-6836.1000117
Boyer R. R., & McKinney J. (2013). Boiling Water Bath Canning Including Jams, Jellies,
and Pickled Products. Virginia Cooperative Extension, 348-594
Mashhadi N. S., et. al. (2013). Anti-Oxidative and Anti-Inflammatory Effects of Ginger in
Health and Physical Activity: Review of Current Evidence. International Journal
of Preventive Medicine, 4 (2013), S36-S42. Retrieved from
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3665023/
Maxwell E. G., Belshaw N. J., Waldron K. W., & Morris V. J. (2012). Pectin - An emerging
new bioactive food polysaccharide. Trends in Food Science &
Technology, 24 (2012) 64-73
Siddiqui, A. A., Bhuiyan, M. H.,& Easdani, M. (2012). Ginger (Zingiber officinale) preserve
and candy development. Bangladesh Research Publications Journal. 7 (2012)
283-290
Sukumaran V., Park S., & Giri S. (2016). Role of dietary ginger Zingiber officinale in
improving growth performances and immune functions of Labeo rohita fingerlings.
Fish & Shellfish Immunology, 57 (2016) 362-370.
DISSERTATION SOURCES
Zhang, M., Viennois, E., Prasad, M., Zhang, Y., Wang, L., Zhang, Z.,Merlin, D. (2016). Edible
Ginger-Derived Nanoparticles: A Novel Therapeutic Approach for the Prevention and
Treatment of Inflammatory Bowel Disease and Colitis- Associated Cancer.
Biomaterials. Elsevier Ltd. https://doi.org/10.1016/j.biomaterials .
Kaur, S. (2011). Isolation, Characterization, Modification and Interaction of Pectin from Citrus
Wastes and Starch from Kidney Bean. Department of Food Science and Technology,
Guru Nanak University. Amritsar-143005
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BOOK SOURCES
Claudio V. S., Guzman M. P., & De Leon S. Y. (2014). Basic Foods for Filipinos. 5th edition.
Manila. Merriam Webster Inc.
Chambers E. IV., (1998). The 9-Point Hedonic Scale. Peryam & Kroll Research
Corporation.
Food and Agriculture Organisation & World Health Organization. (2009). Codex Stan 296:
Codex Standards for Jam, Jellies, and Marmalades. Food and
Agriculture Department. pp 1-10
IMAGE SOURCES
Chaplin M., Pectin. Water Structure and Science.(2016, October 6) Retrieved from
http://www1.lsbu.ac.uk/water/pectin.html
Clemson University. Water Bath Canner. Home Canning Equipment. (2015) Retrieved
from http://www.clemson.edu/extension/hgic/food/food_safety/
preservation/hgic3020.html
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APPENDIX A. Timeline of the Research.
DATE TASK
October 17 Marketing of ingredients
October 18 Requisition of equipment
October 19-20 Sample preparation
October 23 Sensory Evaluation of respondents
October 26-31 Encoding of respondents evaluation
November 01-05 Statistical analysis of the data
Analysis and formulation of results,
November 05-December 02
discussion, and conclusion
December 03 Research Defense
December 03-15 Revisions and consultation
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APPENDIX B. Budgetary Requirements for the research
Printing
Questionnaire PHP 35.00
Consent Forms 35.00
Hedonic Scale 105.00
Article References 200.00
Research 100.00
SUBTOTAL PHP 475.00
Materials
Ginger Rhizome 100.00
Fruit Pectin (Lemon) 300.00
Sugar 100.00
Jar 150.00
SUBTOTAL 550.00
Statistical Analysis 1200.00
Transportation 300.00
Miscellaneous 1000.00
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