LABORATORY REPORT TEMPLATE

Name/s: Centeno, Mark Joseph Berino, Peter Andrew Date: September 21, 2019
Novillos, Julliana Mikaela Mauricio, Juan Daniel
Verueco, Leonor Paula Mendoza, Cazandra Dianne
Ynchausti, Francesca Simone Caramat, Julia

Title:

A. Plant Tissue

Objectives:

A. Describe important features of Plant Tissues

B. Identify basic structure of Plant Tissues
C. Understand how Plant Tissues work

Pre-Laboratory Guide Questions/Problem:

A. Does every Plant Tissue has the same texture?

B. Do every Plant Tissues look alike?
C. Where does Plant Tissues is located?

Hypothesis:

A. Every Plant Tissues have different textures.

B. Plant Tissues are different and does not look alike.
C. Dermal Tissue is found on the outer layer. Ground Tissue makes up the interior of a plant.
Vascular Tissue runs through Ground Tissue inside a plant.

Variables:

A. Independent Variables:
 Mouth Lining
 Leaf
B. Dependent Variables:
 Cheek Cells
 Epidermal Tissue
C. Controlled Variables:
 Extracting the Epidermal Tissue on the leaf.
 Extracting of Cheek Cells.

Materials:
  Rhoeo Discolor Leaf/ Hydrilla Leaf/ Elodea Leaf
 Toothpick with flat end
 Gloves
 Laboratory Gown

Experimental design/Procedure:

1. Extraction of Epidermal Tissue of the leaf using a blade.

2. Viewing the Extracted Epidermal Tissue on the Microscope
3. Extracting of Cheek Cells using Toothpick with flat end from mouth lining.
4. Viewing the Extracted Cheek Cells on the Microscope.
5. Comparing the Epidermal Tissue and Cheek Cells.

Interpretation and analysis of data:

 The Structure of Tissue on the Rhoeo Discolor Leaf is bubble shaped.

 The Structure of the Tissue on Hydrilla and Elodea Leaf is brick shaped.
 Cheek Cells have threads around it.

Conclusions:

Our Conclusion on the Laboratory Experiment is that Different Plants have Different types of tissues.
Plant Tissues can be look alike depending on the other plant you are comparing with it. Plants are
considered as a Prokaryotic cell because it has a Nucleus. Our Cheek Cells belong to Eukaryotic cells
which contains a Nucleus and it is enclosed in a Membrane.
 LABORATORY REPORT TEMPLATE

Name/s: Centeno, Mark Joseph Berino, Peter Andrew Date: September 21, 2019
Novillos, Julliana Mikaela Mauricio, Juan Daniel
Verueco, Leonor Paula Mendoza, Cazandra Dianne
Ynchausti, Francesca Simone Caramat, Julia

Title:
A. Plant Tissue

Objectives:
A. Describe important features of Plant tissues
B. Identify basic structure of Plant tissues
C. Understand how Plant tissues work

Pre-Laboratory Guide Questions/Problem:

A. Does every Plant tissue has the same texture?
B. Do every Plant tissues look alike?
C. Where does Plant tissues is located?

Hypothesis:
A. Every Plant tissues have different textures.
B. Plant Tissues are different and does not look alike.
C. Dermal Tissue is found on the outer layer. Ground Tissue makes up the interior of a plant.
Vascular Tissue runs through Ground Tissue inside a plant.

Variables:
A. Independent Variables:
 Mouth Lining
 Leaf
B. Dependent Variables:
 Cheek Cells
 Epidermal Tissue
C. Controlled Variables:
 Extracting the Epidermal Tissue on the leaf.
 Extracting of Cheek Cells.
Materials:
 Glass slide/ Microscope slide
 Cover slip
 Medicine dropper
 Iodine solution
 Microscope
 Elodea/ Hydrilla/ Rhoeo leaf
 Toothpick with flat end
 Scalpel
 Forceps
 Gloves

Experimental design/Procedure:
A. Leaf Cell
1. Construct a table to organize your observation and drawings.
2. Peel a thin slice of elodea leaf/ hydrilla leaf and place it on the slide.
3. Carefully add drop of methylene blue to the leaf. Avoid getting the stain on your clothes.
Holding the cover slip with your thumb and forefinger, touch it to the surface of the
slide at a 45 degree angle.
4. Focus the cells under low-power magnification. Identify a group of cells that you wish to
study, and move the slide so that the cells are in the center of the field of view.
5. Rotate the nosepiece of the microscope to the higher power objective and view the
cells. Using your fine-adjustment focus, bring the cells into clear view. Draw and
describe what you see. Large structures such as the nucleus, cell membrane, and cell
wall should be visible. Label as many cell structure as you can. Describe the functions of
those cell parts that you observe in the specimen.
6. Rotate the nosepiece back to low-power magnification and remove the slides.
B. Human Cheek Cell
1. Using a clean toothpick, gently scrape the inner lining of your cheeks. Use the flat end
rather than the pointed end of the toothpick. Place the sample on the slide in one
direction only. Do not move the toothpick in both directions for it may cause the cells to
overlap. Add a drop of iodine solution and slowly put the cover slip.
2. Repeat the procedure 3-6 using this specimen.
3. Wrap the plant tissue and the used toothpick in a paper towel and dispose of it in the
garbage. Wash all equipment and return it to its storage area. Wash your hands
thoroughly.

Interpretation and analysis of data:

 The Structure of Tissue on the Rhoeo Discolor Leaf is bubble shaped.
 The Structure of the Tissue on Hydrilla and Elodea Leaf is brick shaped.
 Cheek Cells have threads around it.
Conclusions:
Our Conclusion on the Laboratory Experiment is that Different Plants have Different types of tissues.
Plant Tissues can be look alike depending on the other plant you are comparing with it. Plants are
considered as a Prokaryotic cell because it has a Nucleus. Our Cheek Cells belong to Eukaryotic cells
which contains a Nucleus and it is enclosed in a Membrane.
Analyze and Conclude:
1. Observe the epidermal cells of the leaf and cheek. Describe the general shape of the two (2) cells.

A. Leaf Cells
- Has bubble like texture and brick like texture.
B. Cheek Cells
- Has string like structure around it.

2. What characteristics do the cells have in common? List as many as you can

- They are both microscopic.

- They both produces Ribosomes.
- They both have the similar structure.

3. Identify the unique characteristics of each cell type.

A. Leaf Cells
- a Prokaryotic cell that do not have a true nucleus or membrane-bound organelles.
B. Cheek Cells
- A Eukaryotic cell are cells that contain a nucleus and organelles, and are enclosed by a
plasma membrane.

4. What type of cells do you examine: eukaryotic or prokaryotic? Explain your answer

- The Cells that we examined are both Eukaryotic and Prokaryotic. The Leaf Cell is the Prokaryotic Cell
while the Cheek Cell is the Eukaryotic Cell.

5. Why do you think it was necessary to add methylene blue and iodine solution to the slide?

- It is necessary to add methylene blue and iodine solution to the slide to see how the cell would react so
we could see how it would look like in the microscope.

6. What careers would make use of the techniques learned in this activity?

- Career that make use of the techniques that was used was a Botanist and a Biologist which study plants
and living things.