1 2 3 4

XYLENE 1 XYLENE 2 XYLENE 3 95% ALCOHOL

(5 MINUTES) (5 MINUTES) (5 MINUTES) (10 SECONDS)
→ → → →

5 6 7 8
80% ALCOHOL 70% ALCOHOL WATER HEMATOXYLIN
(10 SECONDS) (10 SECONDS) (1 MINUTE) (10 MINUTES)
→ → → →

10
9 11 12
ACID
WATER WATER EOSIN
(RINSE)
ALCOHOL (RINSE) (10 SECONDS)
→ (QUICK DIP) → →
→

13 14 15 16
WATER 95% ALCOHOL 95% ALCOHOL 95% ALCOHOL
(RINSE) (5 SECONDS) (5 SECONDS) (5 SECONDS)
→ → → →

17 HISTOPATHOLOGY SECTION:
XYLENE
(5 SECONDS)
HEMATOXYLIN & EOSIN STAINING
ST. ELIZABETH HOSPITAL, INC.
*

UNIVERSITY OF THE IMMACULATE CONCEPTION | MEDICAL LABORATORY SCIENCE

2018 INTERNSHIP ROTATION 3: SOLACITO, ROMIE BREN & BALLARA, MARIA RHYGIE