MUHAMMAD NAWAZ SHAREEF UNIVERSITY OF AGRICULTURE, MULTAN

PLANT BREEDING AND GENETICS

COURSE OUTLINE/ TEACHING SCHEDULE
Program M.Sc. (Hons.) Agriculture Major Plant Breeding & Genetics

Semester Spring Semester, 2017-18

Title of the course Advances in Breeding Fiber and Oilseed Crops

Course No. PBG-710

Credit hours 3(2-1)

Course contents THEORY

Genetic diversity utilization in improvement of fiber and oilseed crops. The
worldwide gene pool of fiber and oilseed crops and their improvement.
Molecular basis of evolution of the fiber and oilseed crops. Hybridization and
introgression strategies. Types and construction of fiber and oilseed crops
mapping populations. Genomic tools, resources and approaches.
Deployment of reverse and forward genetics in breeding fiber and oilseed
crops. Fine mapping fiber and oilseed crops. Physical composition and
organization of the fiber and Oilseed crop genomes; genetic and transcript
maps. Bridging classical and genomics-assisted breeding for trait discovery
and development in fiber and oilseed crops. SNP, Next generation
sequencing and genotyping technologies in fiber and oilseed crops. Impacts
of transgenic fiber and oilseed crops on agroecosystems. Role of
transcriptomics, proteomics metabolomics and comparative genomics in
fiber and oilseed crops.

SUGGESTED READINGS
1. Kalloo, G. and B. O. Bergh. (Eds.). 1999. Genetic Improvement of Oilseed
Crops. Pergoman Press, New York. USA.
2. Nagata, T. and S. Tabata (Ed). 2003. Brassica and Legumes - From Genome
Structure to Breeding. Springer Verlag, New York, USA.
3. Ramanath. 2004. Applied Genetics of Oilseed Crops. Daya, New Delhi, India.
4. Verma, D. P. S. 1996. Soybean: Genetics, Molecular Biology and
Biotechnology. Biotechnology in Agriculture Series, No 14. CABI Publishing.
5. Ram. J. Sing. 2007. Genetic Resources, Chromosome, Engineering and Crop
Improvement/Oilseeds. CRC Press Taylor & Francis Group. LLC. USA.
6. Chittaranjan Kole (Ed.). 2007. Genome Mapping and Molecular Breeding in
Plants. Volume 2. Springer Berlin Heidelberg New York. USA.
7. Edwarsd., D, J. Bately, I. Parkin and C. Kole. 2012. Genetics, Genomics and
Breeding of Oilseed Brassicas. CRC Press Taylor & Francis Group. LLC. USA.
8. Andrew. A. Paterson. 2009. Plant Genetic Volume 3. Genetics and genomics
of cotton. Springer Science and business LLC Media. USA.
9. Shiron, J. (Ed.). 2004. Transgenic cotton. Science Pren 16
Donghuangchenggen North Street Beijing 100717, China..
ARTICLES
1. Ste´phane, A. and Jean-Michel, C. 2012. The Significance of Digital Gene
Expression Profiles. GENOME RESEARCH 987.
2. Benjamin A. G. 2010. What Does the Future Hold for Top Down Mass
Spectrometry. J Am Soc Mass Spectrom, 21. 193–202.
 3. Thierry R. and C. Lelong. 2011. Two-dimensional gel electrophoresis in
proteomics: A tutorial. J. Proteom. 7 4. 1 8 2 9 – 1 8 4 1.
4. Lin et al., 2012. Comparison of Next-Generation Sequencing Systems. J.
Biomed. and Biotechnol. 11.
5. Maria et al., 2005. Functional proteomics. Clinica. Chimica. Acta. 357. 140–
150.
6. John, S. C.2011. Protein identification using MS/MS data. J. of Proteomic. 7
4. 1 8 4 2 – 1 8 5 1.
7. Chanchal, K. and M. Mann. 2009. Bioinformatics analysis of mass
spectrometry-based proteomics data sets. FEBS Letters. 583. 1703–1712.
8. Morris et al., 2014. Affinity purification–mass spectrometry and network
analysis to understand protein-protein interactions. Nat Protoc. 9(11):
2539–2554.
9. Puig et al., 2001. The Tandem Affinity Purification (TAP) Method: A General
Procedure of Protein Complex Purification. METHODS. 24. 218–229.
10. Ma, et al., 2014. Application of fluorescence resonance energy transfer in
protein studies. J. Mol. Struct. 1077: 87–100.

Follow up Quizzes (01 mark)

Assignments (03 mark)
Category Major course

•Understand source, genetic basis and utilization of genetic and genomic resources
Objectives fiber and oilseed crops
•Understanding deployment of genomic tools for improvement of fiber and oilseed
crops
Learning Outcomes  Student will be able to know about the basics and advances in
improvement of fiber and oil seed crops

Marks Distribution
Mid (30%) Assignment (10%) Final (60%) Total

12 4 24 40

Name of the Teacher: Dr Sarmad Frogh Arshad

Email I.D. sarmadbiotech@gmail.com

Theory
Week L Contents to be covered Book, chapter Article

1. 1,2 Genetic diversity utilization in Book # 4

improvement of fiber and oilseed crops chapter 2

2 3,4 Genetic diversity utilization in Book # 4

improvement of fiber and oilseed crops chapter 2
3 5,6 The worldwide gene pool of fiber and Book # 3
oilseed crops and their improvement chapter 13
4 7,8 The worldwide gene pool of fiber and Book # 3
oilseed crops and their improvement chapter 13
 5 9,10 Hybridization and introgression Book # 3
strategies chapter 13
6 11,12 Types and construction of fiber and Book # 4
oilseed crops mapping populations. chapter 2
7 13,14 Genomic tools, resources and Book # 6 4
approaches chapter 2
8 15,16 Quiz 1 Book # 6 4
chapter 2
9 Mid Exams
10 17,18 Fine mapping fiber and oilseed crops; Book # 6 4
genetic and transcript maps chapter 2
11 19,20 Bridging classical and genomics-assisted Book # 6 4
breeding for trait discovery and chapter 2
development in fiber and oilseed crops
12 21, 22 SNP, Next generation sequencing and Book # 6 4
genotyping technologies in fiber and chapter 2
oilseed crops
13 23, 24 Impacts of transgenic fiber and oilseed Book # 2
crops on agroecosystems chapter 9
14 25, 26 Role of transcriptomics, proteomics Book # 2
metabolomics and comparative chapter 9
genomics in fiber and oilseed crops
15 27, 28 Role of transcriptomics, proteomics
metabolomics and comparative
genomics in fiber and oilseed crops
16 29, 30 Class Discussion Book # 2
chapter 9
17 31, 32 QUIZ 2 Book # 2
chapter 9
18 Final Exams 1

Dr. Sarmad Frogh Arshad

Assistant Professor
Biotechnology, MNS-UAM